Cambridge International AS & A Level: BIOLOGY 9700/32
Cambridge International AS & A Level: BIOLOGY 9700/32
Cambridge International AS & A Level: BIOLOGY 9700/32
* 7 2 1 0 5 3 7 7 3 6 *
BIOLOGY 9700/32
Paper 3 Advanced Practical Skills 2 May/June 2022
2 hours
You will need: The materials and apparatus listed in the confidential instructions
INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, centre number and candidate number in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
● You should show all your working and use appropriate units.
INFORMATION
● The total mark for this paper is 40.
● The number of marks for each question or part question is shown in brackets [ ].
Total
DC (RW/SG) 303218/3
© UCLES 2022 [Turn over
2
1 Plant tissues contain the enzyme catalase which catalyses the breakdown of hydrogen peroxide
into oxygen gas and water.
You will investigate the effect of changing ascorbic acid concentration on catalase inhibition.
Table 1.1
If H or A comes into contact with your skin, wash off immediately under cold water.
You are also provided with five cylinders of potato tissue, labelled P.
(a) You will need to carry out a serial dilution of the 1 mol dm–3 ascorbic acid, A, to reduce the
concentration by a factor of ten between each successive dilution.
You will need to prepare four concentrations of ascorbic acid in addition to the 1 mol dm–3
ascorbic acid solution, A.
After the serial dilution is completed, you will need to have 9 cm3 of each concentration
available to use.
(i) Complete Fig. 1.1 to show how you will prepare your serial dilution.
Fig. 1.1 shows the first two beakers you will use to make your serial dilution. You will
need to draw three additional beakers.
0 cm3 of W
10 cm3 of
...................
1 mol dm–3
ascorbic acid
...................
...................
...................
...................
...................
...................
Fig. 1.1
[3]
step 1 Prepare the concentrations of ascorbic acid solution, as decided in (a)(i), in the
beakers provided.
step 2 Label test‑tubes with the ascorbic acid concentrations prepared in step 1.
step 4 On a white tile carefully cut the cylinders of potato tissue into thin discs that are
approximately 1–2 mm thick.
step 7 Add 1 cm3 of each concentration of ascorbic acid to the appropriately labelled
test‑tubes.
step 8 Set up the apparatus as shown in Fig. 1.2 using the test‑tube labelled 0. The syringe
barrel should be fully submerged in the beaker of water, B.
bung
delivery tube
test-tube
beaker of
water, B
1 cm3 of 0 mol dm–3
ascorbic acid
syringe barrel
10 potato discs
Fig. 1.2
step 9 Add 5 cm3 of hydrogen peroxide solution H to the test‑tube labelled 0. Place the
bung into the top of the test‑tube, making sure that the syringe barrel stays fully
submerged.
step 10 Record in (a)(ii) the initial volume of gas in the syringe barrel then start the
stop‑clock.
step 11 After 2 minutes record in (a)(ii) the final volume of gas in the syringe barrel. If the
syringe barrel is full of gas, record as 10.
step 12 Repeat step 9 to step 11 with each of the test‑tubes labelled in step 2.
step 13 Calculate the total volume of gas produced at each concentration of ascorbic acid.
Record these processed results in (a)(ii).
(ii) Record your results in an appropriate table, including raw results and processed results.
[6]
(iii) Use your results in (a)(ii) to identify the greatest volume of gas produced in the reaction.
(iv) Describe two improvements to the procedure that would make the measurements more
accurate.
1 ........................................................................................................................................
...........................................................................................................................................
2 ........................................................................................................................................
...........................................................................................................................................
[2]
step 16 Use a thermometer to measure the temperature of H in test‑tube T. Record this value, to
the nearest 0.5 °C, in (b)(i).
step 18 After 2 minutes measure the temperature of the mixture in test‑tube T. Record this value,
to the nearest 0.5 °C, in (b)(i).
(b) (i) State the temperature of H before adding potato discs (step 16). .............................. °C
State the temperature of H 2 minutes after adding potato discs (step 18).
.............................. °C
...........................................................................................................................................
..................................................................................................................................... [1]
(c) A study was carried out in which volunteers were given different daily doses of ascorbic acid
(vitamin C) in addition to their normal diet. The maximum ascorbic acid concentration in the
blood plasma of each volunteer was measured.
Table 1.2
(i) Plot a graph of the data in Table 1.2 on the grid in Fig. 1.3.
Fig. 1.3
[4]
(ii) Suggest an explanation for the results for a daily dose of 0 mg and the results for daily
doses of between 500–1000 mg.
0 mg ...................................................................................................................................
...........................................................................................................................................
500–1000 mg .....................................................................................................................
...........................................................................................................................................
[2]
[Total: 22]
(a) (i) Draw a large plan diagram of the region of the stem on K1 indicated by the shaded
region in Fig. 2.1. Use a sharp pencil.
Use one ruled label line and label to identify the epidermis.
Fig. 2.1
[5]
(ii) Observe one of the larger vascular bundles of the section on K1.
Each xylem vessel element must touch at least two other xylem vessel elements.
• Use one ruled label line and label to identify the wall of one xylem vessel element.
[5]
© UCLES 2022 9700/32/M/J/22
11
(b) Fig. 2.2 shows a diagram of a stage micrometer scale that is being used to calibrate an
eyepiece graticule.
0 10 20 30 40 50 60 70 80 90 100
Fig. 2.2
(i) Use Fig. 2.2 to calculate the actual length of one eyepiece graticule unit.
Fig. 2.3 shows a photomicrograph of a transverse section through a different stem to K1.
This was taken with the same microscope and lenses used to take Fig. 2.2. The eyepiece
graticule has been placed across the diameter of the section.
0 10 20 30 40 50 60 70 80 90 100
Fig. 2.3
(ii) Use the calibration of the eyepiece graticule from (b)(i) to calculate the actual diameter
of the section in Fig. 2.3.
(iii) Identify three observable differences, other than size and colour, between the stem
section on K1 and the stem section on Fig. 2.3.
Table 2.1
[3]
[Total: 18]
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