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Biotechnology

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BIOTECHNOLOGY

Mr. Rey E. Enciso


BIOTECHNOLOGY
BIOTECHNOLOGY
BIOTECHNOLOGY: PRESENT AND
FUTURE
AREAS OF APPLIED BIOTECHNOLOGY:

In 1885, a scientist named Roux


demonstrated embryonic chick
cells could be kept alive outside
an animal's body. For the next
hundred years, advances in cell
tissue culture have provided
fascinating glimpses into many
different areas such as
biological clocks and cancer
therapy.
AREAS OF APPLIED BIOTECHNOLOGY:

Monoclonal antibodies are new


tools to detect and localize specific
biological molecules. In principle,
monoclonal antibodies can be
made against any macromolecule
and used to locate, purify or even
potentially destroy a molecule as
for example with anticancer drugs
AREAS OF APPLIED BIOTECHNOLOGY:

Molecular biology is useful in


many fields. DNA technology is
utilized in solving crimes. It also
allows searchers to produce
banks of DNA, RNA and proteins,
while mapping the human
genome. Tracers are used to
synthesize specific DNA or RNA
probes, essential to localizing
sequences involved in genetic
disorders.
GENETIC ENGINEERING
GENETIC ENGINEERING,

With genetic engineering, new


proteins are synthesized. They
can be introduced into plants or
animal genomes, producing a
new type of disease resistant
plants, capable of living in
inhospitable environments (i.e.
temperature and water
extremes,...). When introduced
into bacteria, these proteins have
also produced new antibiotics
and useful drugs.
WHAT ARE THE USES OF GENETIC
ENGINEERING?
CLONING
Techniques of cloning generate
large quantities of pure human
proteins, which are used to treat
diseases like diabetes. In the future,
a resource bank for rare human
proteins or other molecules is a
possibility. For instance, DNA
sequences which are modified to
correct a mutation, to increase the
production of a specific protein or to
produce a new type of protein can
be stored . This technique will be
probably play a key role in gene
therapy.
USING BIOTECHNOLOGY TO
MODIFY PLANTS AND ANIMALS

Combining DNA from different existing


organisms (plants, animals, insects,
bacteria, etc.) results in modified
organisms with a combination of traits
from the parents. The sharing of DNA
information takes place naturally
through sexual reproduction and has
been exploited in plant and animal
breeding programs for many years.
USING BIOTECHNOLOGY TO
MODIFY PLANTS AND ANIMALS

However, sexual reproduction can


occur only between individuals of the
same species. A Holstein cow can be
mated with a Hereford bull because the
two animals are different breeds of the
same species, cattle. But trying to mate
a cow with a horse, a different species
of animal, would not be successful.
USING BIOTECHNOLOGY TO
MODIFY PLANTS AND ANIMALS

What is new since 1972 is that


scientists have been able to
identify the specific DNA genes
for many desirable traits and
transfer only those genes, usually
carried on a plasmid or virus,
into another organism. This
process is called genetic
engineering and the transfer of
DNA is accomplished using
either direct injection or the
Agrobacterium, electroporation,
or particle gun transformation
techniques.
USING BIOTECHNOLOGY TO
MODIFY PLANTS AND ANIMALS
It provides a method to transfer
DNA between any living cells
(plant, animal, insect, bacterial,
etc.). Virtually any desirable trait
found in nature can, in principle,
be transferred into any chosen
organism. An organism modified
by genetic engineering is called
transgenic. See diagram at the
right.
PRODUCTS OF GENETIC
ENGINEERING
Specific applications of genetic engineering are
abundant and increasing rapidly in number.
Genetic engineering is being used in the production
of pharmaceuticals, gene therapy, and the
development of transgenic plants and animals.
PRODUCTS OF GENETIC
ENGINEERING
PRODUCTS OF GENETIC
ENGINEERING

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