Scribd Logo
Upload

Welcome to Scribd!

  • 40 views

    Introduction To Xenobots

    Uploaded by

    RONAK LASHKARI
    1. The document describes steps to generate a multiple sequence alignment of proteins using ClustalW, which includes picking protein sequences from a database, copying them in FASTA format, uploading the files to ClustalW, downloading the alignment results and phylogenetic tree, and copying the results to a word file. 2. The document provides an example multiple sequence alignment of 4 keratin protein sequences from different organisms. 3. The document provides another example multiple sequence alignment of 3 myoglobin protein sequences from different animal species.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Introduction To Xenobots

    Uploaded by

    RONAK LASHKARI
    40 views7 pages
    1. The document describes steps to generate a multiple sequence alignment of proteins using ClustalW, which includes picking protein sequences from a database, copying them in FASTA format, uploading the files to ClustalW, downloading the alignment results and phylogenetic tree, and copying the results to a word file. 2. The document provides an example multiple sequence alignment of 4 keratin protein sequences from different organisms. 3. The document provides another example multiple sequence alignment of 3 myoglobin protein sequences from different animal species.

    Original Description:

    Xenobots

    Original Title

    Introduction to xenobots

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    1. The document describes steps to generate a multiple sequence alignment of proteins using ClustalW, which includes picking protein sequences from a database, copying them in FASTA format, uploading the files to ClustalW, downloading the alignment results and phylogenetic tree, and copying the results to a word file. 2. The document provides an example multiple sequence alignment of 4 keratin protein sequences from different organisms. 3. The document provides another example multiple sequence alignment of 3 myoglobin protein sequences from different animal species.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    40 views7 pages

    Introduction To Xenobots

    Uploaded by

    RONAK LASHKARI
    1. The document describes steps to generate a multiple sequence alignment of proteins using ClustalW, which includes picking protein sequences from a database, copying them in FASTA format, uploading the files to ClustalW, downloading the alignment results and phylogenetic tree, and copying the results to a word file. 2. The document provides an example multiple sequence alignment of 4 keratin protein sequences from different organisms. 3. The document provides another example multiple sequence alignment of 3 myoglobin protein sequences from different animal species.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 7

    NAME:- PARMAR JANHVI RANJITBHAI

    IAR NO:- 12690


    SUBJECT:- BIOINFORMATICS ASSIGNMENT
    MSC BIOTECHNOLOGY

    Steps to generate multiple sequence alignment of protein


    using ciustal W
    1. pick different protein sequence from protein database
    2. copy protein sequence in fasta format
    3. paste all sequence in notepad
    4. upload all file in culstel W
    5. do multiple sequence aligment
    6. download result of multiple sequence aligment
    7. generate phylogenetic tree
    8. download phylogenetic tree
    9. copy all result in word file
    1.keratin [Pseudomonas mendocina]
    GenBank: ARS51075.1
    Identical Proteins FASTA Graphics
    LOCUS ARS51075 200 aa linear
    DEFINITION keratin [Pseudomonas mendocina].
    ACCESSION ARS51075
    VERSION ARS51075.1

    CLUSTAL 2.1 multiple sequence alignment

    ARS51075.1 ------------MPAPLLTRCTLLLGLLLP--------LSGAAAELYRYVDDKGVTVLSR
    KKJ97415.1 ---------------------MLLLGVICP--------VLASAAELYRYVDDRGVVVLDR
    KMN03504.1 ------------MVGQDVMRCLMALGLVLQGLSFGAQAAPGGAVELYRYVNDKGVVVLDR
    KPL63462.1 MPFWRAQKQCPSGALQRLVRGSGALMLWLSVASTIVQAADVPEVRLYRYIDSRGVTVIDR
    * : ..****::.:**.*:.*

    ARS51075.1 QGVPPEFIGKGYEVLNDQGRVLRVIPPAPTPEEFARMQADKARASNDAQLLRLYTNLDDV
    KKJ97415.1 HGVPPQHISRGYQVLNEQGRVVREVPPAPTAEEFARLQAQKARAASDAQLLRLYASVEDV
    KMN03504.1 QGVPPQYIGKGYQVLNDQGRVIRTVPPAPTAEELRQRKAAQAQASSDAQLLRLYSSLEDV
    KPL63462.1 LGVPAEYVAKGYEVLNARGRVVQVIPPAPTAEQIRQTEAAKLQAEANAKLLSLYGSVEEV
    ***.:.:.:**:*** :***:: :*****.*:: : :* : :* :*:** ** .:::*

    ARS51075.1 DRARERKLAELDGVTSVARGNLQSVRTQQANLQSQAAEHERAGRQVPEHLLAQINNLKEE
    KKJ97415.1 ERAETRKLSELDSVMGLARGNLQSIRNQHASLRKQAANHERAGRKVPGNLLAQIENLEKE
    KMN03504.1 DRARERRLAELDGLSSVARGNLQSLKLQQANLQGQAANQERAGRPVAQALVDQLDDLKQE
    KPL63462.1 DRVKTRKLAELDTLIDVAQGNIQGLANQQRSLQGQAADQERAGRPVPQAIIEQLDDLRSQ
    :*.. *:*:*** : .:*:**:*.: *: .*: ***::***** *. :: *:::*..:

    ARS51075.1 QQRLMRDIARYREARTQAEADFAADRARLAELFGEPQAKP
    KKJ97415.1 EQSLQRDLKRFEKARADAEVSFASDRQRIAELLGQKQ---
    KMN03504.1 EKRLQGEIGRFQKAREDAERTYAADRARLSQLLGVSP---
    KPL63462.1 QQKLQADIAGYQAARTRAEADFAADRVRVQQLTR------
    :: * :: :. ** ** :*:** *: :*

    Pseudomonas mendocina: ARS51075.1

    Stutzerimonas stutzeri KKJ97415.1

    Pseudomonas viridiflava KPL63462.1

    Pseudomonas aeruginosa KMN03504.1


    Pseudomonas mendocina

    Stutzerimonas stutzeri

    Pseudomonas
    aeruginosa

    Pseudomonas viridiflava

    2.insulin – elephant
    DEFINITION insulin - elephant.
    ACCESSION INEL
    VERSION INEL

    CLUSTAL 2.1 multiple sequence alignment

    pir||INEL ----------------------------------------------FVNQHLCGSHLVEA
    pir||INMKSQ ----------------------------------------------FVNQHLCGPHLVEA
    XP_011213888.1 MMFPKHTVNFPNLLCLSLVVISFVFCVELSPHCTEALPLLPGGSNDVEFKRYCSTNLSDA
    GFR87518.1 --MQTPWTCFVKCCVVLATTALLILSLQTKGSEAGLMHICDFNQRRPHPNGICGRRLPLV
    : *. .* .

    pir||INEL LYLVCG--------------------------------------ERGFFYTPKTG-----
    pir||INMKSQ LYLVCG--------------------------------------ERGFFYAPKTG-----
    XP_011213888.1 IRLICGGRYYSLS-----------RKFPDSVGMGHVSSLKRLAGEEGEYRQPFQG-----
    GFR87518.1 LAGLCHRFTMLMRNSGSAKTTRKRSAAASASDMELSNDLLQDKEDQMQLLAPIIKDMTPG
    : :* :. *

    pir||INEL ----------------------------------IVEQCCTGVCSLYQLENYCN-----
    pir||INMKSQ ----------------------------------VVDQCCTSICSLYQLQNYCN-----
    XP_011213888.1 ----------------------------------AIHECCRRPCGYPELKSYCEPDY--
    GFR87518.1 DEAATGPTKDQLESIFLDKKSALGYLAKRDDSGDIVCECCYHQCSVLELLQYCELPANL
    : :** *. :* .**:

    Bactrocera dorsalis : XP_011213888.1


    Elysia marginata : GFR87518.1
    common squirrel monkey : INMKSQ

    Bactrocera dorsalis

    Elysia marginata

    common squirrel monkey

    elephant
    3.Myoglobin [Camelus dromedarius]
    LOCUS KAB1270346
    DEFINITION Myoglobin [Camelus dromedarius].
    ACCESSION KAB1270346
    VERSION KAB1270346.1

    CLUSTAL 2.1 multiple sequence alignment

    KAB1270346.1 MGLSDGEWQLVLNVWGKVEADVAGHGQEVLIRSHVWGECPVLPALLSGVRALSESHQKRL
    ALJ77201.1 ------------------------------------------------------------
    ALJ77193.1 ------------------------------------------------------------
    ALJ77194.1 ------------------------------------------------------------

    KAB1270346.1 RKDSRDDDGDDGDGDNDNDDGDGDDDDGDDDGDNDNDDDDGDGDDDGDGDDDRDDSDGGG
    ALJ77201.1 ------------------------------------------------------------
    ALJ77193.1 ------------------------------------------------------------
    ALJ77194.1 ------------------------------------------------------------

    KAB1270346.1 GDHADDDNGNDDGDDDGHPETLEKFDKFKHLKTADEMKASEDLKKHGNTVLTALGGILKK
    ALJ77201.1 ------------------------------------------------------------
    ALJ77193.1 ------------------------------------------------------------
    ALJ77194.1 ------------------------------------------------------------

    KAB1270346.1 KGHHEAELKPLAQSHATKHKIPVKYLEFISDAIIHVLQSKHPGDFGADAQAAMNKALELF
    ALJ77201.1 ----------------------VKYLEFIS------------------------------
    ALJ77193.1 ----------------------VKYLEFIS------------------------------
    ALJ77194.1 ----------------------VKYLEFIS------------------------------
    ********

    KAB1270346.1 RNDMAAKYKELGFQG
    ALJ77201.1 ---------------
    ALJ77193.1 ---------------
    ALJ77194.1 ---------------

    Melozone leucotis nigrior: ALJ77201.1


    Melozone kieneri : ALJ77194.1
    Melozone fusca : ALJ77193.
    Melozone kieneri

    Camelus dromedarius

    Melozone fusca

    Melozone leucotis nigrior

    You might also like