TRIANGLE BIOMEDICAL SCIENCES, INC.

3014 Croasdaile Drive, Durham, NC 27705 USA Ph: (919)384-9393 Fax: (919)384-9595 E-Mail: [email protected]

Minotome Plus
TM

OPERATION MANUAL
OM2563
Version 1.1

TRIA
NGL
E BIO
MED
ICA
L SC
IEN
CES
, INC
.

Microtome Cryostat
Cat # 2563 - 115V, 60Hz; 100V, 50Hz
Cat # 2564 - 240V, 50Hz; 200V, 50Hz

Minotome Plus Operation Manual 1

1-21-98
 Table of Contents

1.0 SIMPLIFIED OPERATING INSTRUCTIONS..................................... 3

2.0 INSTALLATION.............................................................................. 5
2.1 Microtome Adjustment...................................................... 6
Microtome Alignment ....................................................... 6
2.2 Disposable Blade Holder Installation................................. 7
2.3 Positioning of Knife Holder Assembly............................... 7
2.4 Specimen Holder Angle Adjustment.................................. 7
2.5 Optional Knife................................................................... 8
2.6 Anti-Roll Plate Alignment................................................... 8
2.7 Optional Index Post........................................................... 10

3.0 OPERATION.................................................................................. 11
3.1 Warnings and Cautions.................................................... 11
3.2 Control Panel.................................................................... 12
3.3 Sectioning......................................................................... 14
First Use........................................................................... 14
Temperature Selection...................................................... 14
Thickness Selection.......................................................... 14
Sample Preparation........................................................... 14
Cutting.............................................................................. 15
3.4 Troubleshooting................................................................ 16

4.0 MAINTENANCE.............................................................................. 17
4.1 Cleaning............................................................................ 17
4.2 Decontamination............................................................... 18
4.3 Spare Parts....................................................................... 19
4.4 Lubrication........................................................................ 19
4.5 Warranty........................................................................... 21
4.6 Condition of Returned Equipment..................................... 21

5.0 SPECIFICATIONS.......................................................................... 22

5.1 Temperature Selection Table............................................. 23

2 Minotome Plus Operation Manual

 1.0 SIMPLIFIED OPERATING
INSTRUCTIONS

General The TBS Minotome Plus™ is designed for use as a cryogenic sectioning
device in clinical, pathological and research applications. Do not attempt to
use the instrument unless you are professionally trained in the use of similar
devices.

Installation Roll the cryostat to the desired location and lock the front casters. Allow a
minimum of 4 inches clearance on all sides of the cryostat for proper
ventilation. Allow additional clearance on the right side for easy rotation of the
handwheel. The international model (Cat. NO. 2564) is shipped for use at
240 VAC. To use it on a different voltage source the autotransformer must be
reconfigured. A qualified electrical technician should use an AC Voltmeter
to measure the voltage at the installation site. After removing the rear panel,
relocate the BROWN wire to the autotransformer tap which is closest to the
measured line voltage. Replace the rear panel. To minimize line load
variations, the Minotome Plus™ should be connected to a dedicated line. The
line voltage supply must be a standard three-wire, grounded, single-phase
circuit.

Operation Select an appropriate chamber temperature. Insert a disposable blade

(H-DMB-HD) into the Shur/Sharp™ disposable blade holder (already installed
in the cryostat). Loosen the thickness lock at the top of the index post and
rotate the index so a setting lines up with the etched guide. Settings of 1
through 8 may be selected, where 1 corresponds to 2 microns and 8
corresponds to 16 microns. Place the specimen holder into the ball and chuck
assembly so the notch on the outer edge of the specimen holder lines up with
the knurled screw. Turn the knurled screw until it is finger tight.

Advance the sample until it is approximately 1/8 inch from the cutting edge
using one of the following methods:

1. Motorized: Press the ADVANCE/RETRACT switch downward

and hold.
2. Manual: Turn the large ratchet wheel at the rear of the
microtome clockwise, being careful of the blade.
3. Knife Holder: Loosen the knife holder assembly locking lever by
moving it to the right and slide the assembly forward.
Return the locking lever to the original locking position.
Rotate the anti-roll plate away from the disposable blade. Ensure that the
ratchet pawl on the large ratchet wheel at the rear of the microtome is in the
'engaged' position (toward the right). Rotate the handwheel several times until
the blade contacts the sample. Make several initial cuts until the desired
sectioning face is obtained. Rotate the anti-roll plate back into position to begin
sectioning.

Minotome Plus Operation Manual 3

 Warnings & Cautions
Below are listed important operating procedures. Be certain to follow the
instructions carefully.

• Do not locate the Minotome Plus™ in direct sunlight or near any air-
handling ducts.

• The microtome should always be lubricated after cleaning and/or monthly .

Do so at operating temperature. Lubricate with TBS's Shur/Lube™ (H-LUB) .

• When sectioning for extended periods of time, the chamber temperature

may rise. Close the window and allow the cryostat time to recover.

• There is no automatic stop to prevent the main feed screw from

advancing too far (completely out). Retract the assembly regularly to
avoid damage to the microtome.

• Always put the ratchet lever in the 'manual' position before retracting, in
order to avoid damaging the microtome motor.

• Before decontaminating the microtome, be sure to remove the motor

assembly from the rear of microtome.

• The Shur/Sharp™ disposable microtome blades are very sharp. To

prevent injury, use extreme caution when handling.

• Carefully remove and properly discard spent blades.

• If infectious or radioactive material have been sectioned, immediately

sterilize or decontaminate the Minotome Plus™ and its components.

• The power cord must be plugged into a grounded power outlet. Never
remove the grounding prong from the power plug or use any adapter
which defeats, or does not complete, the power ground circuit.

• The quick freeze plate is extremely cold and is active whenever the
compressor is running. Prolonged contact with unprotected skin may
result in burns.

Warranty/ TBS warrants for a period of one year (12 months) from the date of shipping,
Service under normal and proper usage, all of its products against defects in
workmanship and material, and will repair or replace the same without charge
when shipped prepaid to TBS' factory in Durham, NC. User is required to
return the warranty card to TBS within ten (10) days after receipt of the
product in order to validate the warranty. Warranty service for international
shipments is handled by the selling agent. For further information, please call
(919)384-9393.

4 Minotome Plus Operation Manual

 2.0 INSTALLATION

Follow the instructions on the outside of the shipping carton to unpack the cryostat. The
Minotome Plus™ is shipped with the microtome installed in the cryostat chamber and the Shur/
Sharp™ Blade Holder and anti-roll device in position.

Roll the cryostat to the desired location and lock the front casters by pushing the small locking
lever on the top of each wheel with your foot. Allow a minimum of four inches clearance on all
sides of the cryostat for proper ventilation. Allow additional clearance on the right side for
easy rotation of the handwheel.

The international model (Cat. No. 2564) is shipped for use at 240 VAC. To use it on a different
voltage source the autotransformer must be reconfigured. Only a qualified electrical
technician should make the following adjustments: Using an AC Voltmeter, measure the
voltage at your site. Remove the rear panel. Relocate the BROWN wire to the autotransformer
tap which is closest to the measured line voltage. Replace the rear panel.

To minimize line load variations, the Minotome Plus™ should be connected to a dedicated line.
The line voltage supply must be a standard three-wire, grounded, single-phase circuit.

Warning: The power cord must be plugged into a grounded power outlet. Never
remove the grounding prong from the power plug or use any adapter which defeats, or
does not complete, the power ground circuit.

The power cord provided with the Minotome Plus™ is correctly rated for the highest
current demand. This power cord should never be interchanged with cords from
equipment with lower current demand. Incorrect power cords can create a fire hazard.

Plug the cryostat in and review the Control Panel settings (section 3.2). If you encounter difficulty
sectioning, follow the more detailed Microtome Adjustment instructions (section 2.1).

Minotome Plus Operation Manual 5

2.1 Microtome Adjustment

The microtome is installed in the cryostat at the factory, but may require some
adjustment on-site for proper sectioning. The following adjustments may be
made to correct a common problem of 'thick and thin' or inconsistent
sectioning.

Warning: Disposable microtome blades and reusable knives are

extremely sharp. To prevent injury, use extreme caution when
handling.

Microtome Alignment Although the microtome is installed at the factory, its alignment may shift
during transport. Misalignment results in a 'clunking' sound when the
handwheel is turned. To realign, loosen the two large black thumbscrews
which secure the microtome to the cryostat. Loosen the two set screws that
couple the outer handwheel shaft to the inner microtome shaft. Pull the
handwheel away from the cryostat. Check that the handwheel is securely
fastened to the outer shaft, by holding each and turning to feel for any 'play'
between them. The handwheel is secured by two set screws which must be
aligned with flat surfaces on the outer shaft. If any looseness is found, align
and tighten the set screws. Slide the handwheel back toward the cryostat,
taking care to align the set screws in the outer handwheel shaft with the flat
surfaces of the inner microtome shaft, and tighten. Rotate the handwheel and
reposition the microtome until no 'clunking' sound is heard. Then, while
rotating the handwheel slowly, alternately tighten down the large black thumb
screws in small increments. Rotate the handwheel. Feel for any binding. If
the turning action is difficult, the outer shaft may need to be freed up by
loosening the wedge (outside of cabinet) or seal (inside cabinet). Once either
is loosened, rotate the handwheel to free up the shaft and secure the wedge or
seal back in place (both should be centered about the shaft).
Set Screws
Seal
Inner Shaft

Handwheel

Set Screws
Outer Shaft Wedge

Handle

Handwheel Lock

Coupling

6 Minotome Plus Operation Manual

2.2 Disposable Blade Holder Installation
A Shur/Sharp™ Disposable Blade Holder and blades are standard items
supplied with the Minotome Plus™ . The anti-roll plate is configured for use
with the disposable blade system. To reconfigure the anti-roll mechanism for
use with reusable knives, see section 2.6. Specimen
Holder

Warning: Disposable blades and

reusable knives are extremely
sharp. To prevent injury, use Disposable Blade
Holder or Knife
extreme caution when handling.

Back the two knife holder screws out

Knife
until they are no longer visible from the Knife
Clamp
Holder
Screw
front of the unit. Advance one knife
holder screw until one thread is visible.
(Some units may require up to two
threads to be visible.) Secure screw Lock Nut

in place by tightening the lock nut

around it. Repeat procedure for remaining Holder Bracket
screw. Tighten both knife clamps down.

2.3 Knife Holder Assembly Adjustment

When the "Knife Holder Assembly" locking
lever (at front and bottom of knife holder
assembly) is tightened down (slid left), the
entire assembly should be immobile (no
sideways motion, unable to slide forward or
backward). If it is mobile, remove the assembly
from the microtome base and slide the locking
lever to the right. Using a flathead screwdriver,
rotate the center bolt counter-clockwise (not too
tight). Be sure that the locking lever can still
be tightened by sliding it left. Locate the set
screw beneath the front of the assembly, and
ensure that it is tight. Replace the assembly in
the microtome base.
2.4 Specimen Holder Angle Adjustment Locking Lever

Install the specimen orientation ball

assembly and detachable specimen
holder as shown. Lightly tighten the
specimen orientation handle so that the
ball assembly is still moveable. Be
certain the knurled screw is tight.
Position the loose specimen
orientation ball assembly so that the
detachable specimen holder is
completely vertical with respect to the
blade edge facet. (Place a flat ruler
vertically on top of the knife holder
screws and slide the knife holder
forward to engage the loose
assembly.) Tighten the specimen
orientation handle to set the angle.

Minotome Plus Operation Manual 7

2.5 Optional Knife
The resharpenable knife (Cat# 3257) is an option and can be used as
an alternative to the Disposable Blade System. A modification of the
anti-roll plate is required (see section 2.6). The installation and
cutting angle adjustment procedures are similar for reusable knives
and the Shur/Sharp™ disposable blade system although the knife
holder screws may require more threads showing in order to
compensate for the different cutting angles of reusable knives.
2.6 Anti-Roll Plate Alignment
Proper adjustment of the anti-roll plate may be made once the upper
edge of the plate is aligned parallel to the edge of the disposable blade
or reusable knife. To accomplish this, loosen the two slotted screws on
the right side mounting support. The assembly can then be shifted until
the edges are parallel. Tighten the two slotted screws once the plate is
aligned.

The height of the anti-roll plate relative to the

blade is also important. If the plate is too high,
the sample will hit the plate instead of the blade.
If the plate is too low, the sample will curl up in
front of the plate. The height is adjusted by first
loosening the small thumbscrew lock. Next, turn
the adjustment screw by its rubber cap to raise or
lower the plate. Once the samples feed smoothly
between the plate and blade, tighten the thumb-
screw lock.ppppppppppppppppppppppppppppp
Note: A dirty or damaged (nicked upper edge) anti-roll
Slotted Screws
plate will cause the sample to tear or bind. Additional
Round-head Screw
anti-roll plates (Cat#3267A) can be ordered.
(Acorn Nut)
All anti-roll plates are shipped from the factory configured for the Disposable
Blade Holder.

To reconfigure the anti-roll plate assembly for reusable knives:

1. Loosen (do not remove) the round-head screw (acorn nut) on the right
side of the anti-roll plate mounting support.

2. Remove the anti-roll plate assembly. Be careful to restrain the spring while
disassembling. Wear protective glasses. Take it to a bench and rework
it as follows:
- Use needle-nose pliers or forceps to remove the E-ring.
- Remove anti-roll plate, plastic washer and spring.
- Reassemble the anti-roll plate parts in the following order: first
put anti-roll plate; followed by the spring, next the plastic washer,
and finally put the E-ring back into its original slot to hold the parts
together.

3. Put the reworked anti-roll plate assembly back into its mounting supports so
that the thumbscrew lock now faces up. This is very important because
the anti-roll plate is now inverted from its original position. Turn the
round-head screw just enough to hold the anti-roll plate in place, but do not
tighten them against the side of the anti-roll plate support yet.

8 Minotome Plus Operation Manual

 Install the reusable knife as follows:

1. Loosen both knurled knife-clamp finger screws.

2. WARNING: The top edge of the knife is very sharp. Very carefully remove
the knife. Place it so that it is centered between the microtome knife-
clamp fingers.
3. Tighten both knurled knife-clamp finger screws.

4. Use the anti-roll adjust and lock knobs to align and lock the anti-roll plate just
above the knife edge to prevent specimen curling. The anti-roll plate should be
parallel to the knife edge. If it is, tighten both slotted screws on the right side of
the anti-roll plate mounting support. If not, adjust it as follows:
- Loosen, but do not remove, both slotted screws on the right side of the
anti-roll plate mounting support.
- Adjust the anti-roll plate so that it will be parallel to the knife edge.
- Tighten both slotted screws.

NOTE: If sections curl up in front of the anti-roll plate, then the plate must be
adjusted forward. If a specimen hits the plate, then the plate is too far forward and
must be adjusted backward.

NOTE: The knife angle may need readjustment.

At this point, you are ready to start sectioning.

IMPORTANT: Do not store or leave a blade in place for extended periods.

Leave the blade holder clamp in the open position when not in use.

Minotome Plus Operation Manual 9

2.7 Optional Index Post
Optional index post Cat. No. 3347 allows the user to section 18 to 32 µm or 34
to 42 µm. To install, locate and loosen the set screw at the rear of the
microtome base which secures the index post position. Remove the original
post, and install the optional post with the flat portion toward the rear and the
red lines facing the user. Adjust the height of the post so one red line is flush
with the top surface of the base. Use lower red line for 18 to 32 µm and the
upper red line for 34 to 42 µm. Rotate the handwheel and listen to ensure that
the correct number of 'clicks' can be heard for each setting (see table).

Standard Index Post - 2 to 16 µm

Setting No. of Clicks Microns Advanced
1 1 2
2 2 4
3 3 6
4 4 8
5 5 10
6 6 12
7 7 14
8 8 16

Optional Index Post - 18 to 32 µm (lower red line)

Setting No. of Clicks Microns Advanced

1 9 18
2 10 20
3 11 22
4 12 24
5 13 26
6 14 28
7 15 30
8 16 32

Index Post - 34 to 42 µm (upper red line)

Setting No. of Clicks Microns Advanced
1 17 34
2 18 36
3 19 38
4 20 40
5 21 42*
*Maximum setting. (See Caution)

*Caution: Do not set the microtome to advance more than 42

µm. A greater setting will not allow the specimen to
clear the blade before advancing.

10 Minotome Plus Operation Manual

 3.0 OPERATION

3.1 Warnings and Cautions

• Disposable blades and reuseable knives are very sharp. To prevent
injury, use extreme caution when handling.

• If infectious or radioactive material has been sectioned, IMMEDIATELY

sterilize or decontaminate the Minotome Plus™ and its components.

• To avoid electrical shock, the power cord must be plugged into a grounded
power outlet. Never remove the grounding prong from the power plug or use
any adapter which defeats, or does not complete, the power ground circuit.

• The quick freeze plate is extremely cold, and is active whenever the
compressor is running. Prolonged contact with unprotected skin may result in
burns.

• Do not position the Minotome Plus™ in direct sunlight, or near any air-
handling ducts. Allow four inches of space on all sides of the Minotome
Plus™ for proper ventilation.

• The microtome requires periodic lubrication, based on usage. It should

always be lubricated after cleaning. The procedure should be done once it has
reached operating temperature.

• Lubricate the microtome with Shur/Lube™ (H-LUB), a specialty chemical

formulation designed to lubricate the various metals utilized under sub-zero
operating conditions.

• When sectioning for extended periods of time, the chamber temperature

may rise. Close the window and allow the cryostat time to recover if the
sections become mushy.

• There is no automatic stop to prevent the main feed screw from advancing too
far (completely out). Retract the assembly regularly to avoid damage to the
microtome.

• The ratchet lever must be in the 'manual' (toward the left) position to allow the
specimen holder assembly to retract. The motor will not be able to retract
otherwise, and will emit a loud noise. Always put the ratchet lever in the
'manual' position before retracting to avoid damaging the microtome motor.
Return the ratchet lever to the right "engaged" for continued cutting.

• Before decontaminating the microtome, be sure to remove the motor assembly.

It may not be compatible with some processes of decontamination. See Section
4.2 Decontamination.

Minotome Plus Operation Manual 11

3.2 Control Panel

The front control panel of the Minotome Plus™ is used to set chamber
parameters and activate the chamber controls.

Press the CHAMBER LIGHT button to illuminate or shut off the fluorescent
CHAMBER lamp inside the cutting chamber. The chamber light is "on" when the light in
LIGHT the button is illuminated

Press the QUICK FREEZE button to deliver refrigerant to the quick freeze bar
on the left side of the chamber for 10 minutes to provide rapid sample
preparation. When pressed again, this function is terminated. The quick
QUICK freeze is activated when the light in the button is illuminated. (Note: During
FREEZE normal cooling the refrigerant is cycled through the quick freeze plate. This
function activates refrigeration when it has cycled off or extends it for a period
of 10 minutes. Once stopped, there is an intentional three minute delay before
the compressor can be re-started. )

Press the MANUAL DEFROST button to activate a 3 minute (approx.) defrost

cycle. A defrost cycle may be terminated by pressing the button again.
MANUAL Periodic manual defrost, in addition to the automatic defrost, is recommended
DEFROST if climate conditions are hot and/or humid. Following a defrost, some ice will
remain in the drip tray below the evaporator at the rear of the chamber. When
the light in this button is illuminated, a defrost is in progress.

Press the FREEZE PLATE TEMP button to view the quick freeze plate
FREEZE
PLATE temperature. An illuminated light in this button indicates the quick freeze plate
TEMP. temperature is displayed.

Press the CHAMBER TEMP button to view and change the desired chamber
temperature. If the display is indicating the freeze plate temperature then
pressing the CHAMBER TEMP button returns the display to the actual
chamber temperature. Pressing the CHAMBER TEMP button again will cause
the display to flash, indicating it is in the change mode. Use the up and down
CHAMBER
TEMP. arrow keys to change the display to the desired temperature. When the
desired temperature is reached, simply press the CHAMBER TEMP button
again and the display will revert to the actual chamber temperature. An
illuminated light in this button indicates the actual chamber temperature is
being displayed.

12 Minotome Plus Operation Manual

 The arrow buttons are used along with the time and temperature control
buttons for setting the desired parameters.

The DEFROST SET button is used to select the desired automatic defrost
time. This time is set for 1:00 AM from the factory. If a different time is
desired, press the DEFROST SET button once and use the up and down arrow
DEFROST keys to change the hour display to the desired time. Press the DEFROST
SET SET button again and use the arrow keys to change the minute display. When
the desired defrost time is reached, simply press the DEFROST SET button
and the display will revert to the current time. The automatic defrost time is in
the change mode when the light in the button is illuminated

The CLOCK SET button is used to change the displayed time. The time is set
for Eastern Standard Time from the factory. If a different time is required, press
the CLOCK SET button and use the up and down arrow keys to change the
CLOCK hour display. Press the CLOCK SET button again and use the arrow keys to
SET change the minute display. When the current time is reached, simply press
the CLOCK SET button and the display will indicate the current time. An
illuminated light in this button indicates the current time displayed is in the
change mode.

The RETRACT/ADVANCE switch is used to advance or retract the specimen

holder assembly. The specimen holder assembly can be advanced at any
time. To ensure rigidity when sectioning, the specimen holder assembly
should never be extended more than 1/2 inch.

Caution: There is no automatic stop to prevent the main feed

screw from advancing too far (completely out). Retract
the assembly regularly to avoid damage to the
microtome.

To coarse advance, press and hold the toggle switch downward. To advance
approximately 20 µm only, depress the toggle switch once, and release.

Minotome Plus Operation Manual 13

 The specimen holder assembly can only be retracted if the ratchet pawl is in
the 'manual' position.

Caution: The ratchet pawl must be in the 'manual' position to

allow the specimen holder assembly to retract. The
motor will not be able to retract otherwise, and will emit
a loud noise. Always place the ratchet pawl in the
'manual' position before retracting to avoid damaging
the microtome motor.

Once the ratchet pawl is in the 'manual' position, press and hold the toggle
switch upward to retract the specimen holder assembly. Once it is fully
retracted, the motor will emit a loud noise indicating it cannot retract any
further.

3.3 Sectioning
TM
First Use When the Minotome Plus has been plugged in, and a chamber temperature
has been selected, it takes approximately one hour to achieve an air
temperature of -20°C. It will take several hours for all the metal mass of the
microtome and blade holder to reach equilibrium. For best results, do not rush
the process. Once set temperature is reached, the microtome may require
adjustment (see Section 2.1 Microtome Adjustment) and lubrication (see
Section 4.4 Lubrication).

Temperature Selection Select an appropriate chamber temperature according to the chart at the rear
of this manual. If the sample fragments or splits when sectioning, the se-
lected temperature may be too cold. If the selected temperature is too warm,
the sample will seem soft and sections will collect at the blade edge.

Thickness Selection The index post is used to set the desired section thickness. Loosen the
thickness lock at the top of the index post and rotate the index adjust so a
setting lines up with the etched guide. Settings of 1 through 8 may be
selected. Refer to the tables in Section 2.7 to identify thickness by setting
number.

Sample Preparation The holes in the quick freeze plate on the left side of the chamber can
accommodate up to 13 specimen holders for sample preparation. When the
refrigeration system is on, the quick freeze plate temperature is approximately
-40°C. If it is not on, press the QUICK FREEZE button on the control panel
and the system will cycle on (a three minute delay between cycles may slow
TM
the start). Place a small amount of embedding medium (TFM ) on a
specimen holder. When it starts to turn white and solidify, place the specimen
on the plate and surround it with more embedding medium. Allow it to freeze
thoroughly before sectioning. The heat extractor (Cat#2085) can be used to
accelerate the process.

Sample Position The specimen holder angle should be vertical (see Section 2.1 Microtome
Adjustment) before sectioning. Place the specimen holder into the ball and
chuck assembly so the notch on the outer edge of the specimen holder lines
up with the knurled screw. (When knurled screw is tightened it should engage
a small hole. This prevents looseness in the section mounting.) Tighten the
knurled screw finger tight.

14 Minotome Plus Operation Manual

 The sample can be brought closer to the blade edge by one of the following
methods:

Motorized: Press the ADVANCE/RETRACT switch downward and hold.

Manual: Turn the large ratchet wheel at the rear of the microtome
clockwise, being careful of the blade.

Knife Holder: Loosen the knife holder locking lever by moving it to the
right, and slide the knife holder assembly forward.

Use any method to advance until the Disposable Blade Edge is approximately
1/8 inch from the sample. Rotate the anti-roll plate away from the blade.

Cutting Ensure that the ratchet pawl is in the 'automatic' position (toward the right).
Rotate the handwheel several times until the blade engages the sample. Note:
To advance more quickly using the handwheel, rotate the handwheel from 12
o'clock to 3 o'clock and then back to 12. Each of these forward movements
advances the sample without making a pass by the blade.
Make several 'rough' cuts until the desired sectioning face is obtained. Rotate
the anti-roll plate back into position to begin sectioning.

At this point, each turn of the handwheel will produce one section.

Minotome Plus Operation Manual 15

3.4 Troubleshooting
Problem Possible Cause Corrective Action

No Lights Blown fuse on circuit board Call for service

No Cooling Refrigerant problem Call for service

Chamber temp. rising Sectioning too long Close window

Evaporator Blocked Defrost

No motor action Loose wiring Check motor connector

Microtome not advancing Ratchet pawl in 'manual' position Move to 'automatic'

(no clicks heard) Weak pawl spring Replace spring
Frost on ratchet wheel Clean ratchet wheel

Inconsistant sections Loose blade holder Tighten locking lever

(Thick and Thin) Loose specimen Tighten specimen holder screw
Dull Blade Replace/resharpen
Improper Blade Holder angle Adjust angle (see Sec. 2.2)
Loose Microtome Tighten thumbscrews/set screws

Sections fragment Too cold Raise chamber temperature

Knicked or damaged Anti-roll plate Replace anti-roll plate

Sections gather Too warm Lower chamber temperature

(at knife edge) Anti-roll plate incorrectly positioned Adjust height (see Sec. 2.6)
Knicked or damaged Anti-roll plate Replace anti-roll plate
Dull Blade Replace/resharpen

Handwheel hard to turn Dry Microtome Lubricate

Shafts misaligned Align (see Sec. 2.1)
Wedge or seal binding Adjust (see Sec. 2.1)

16 Minotome Plus Operation Manual

 4.0 MAINTENANCE

4.1 Cleaning
The Minotome Plus ™ should be unplugged and allowed to warm up to room
temperature before cleaning. Remove the disposable blade system and any
blades stored in the chamber. To prevent rusting, run them under warm water
until they are room temperature and dry thoroughly. Remove rubber drain plug
from bottom of inner chamber.

Warning: The microtome disposable blades are very sharp.

To prevent injury, use extreme caution when handling.

Clean the outside of the Minotome Plus™ and the inside of the chamber with
a damp sponge using a mild detergent (non-abrasive).

The microtome must be removed for cleaning. Loosen the two set screws that
couple the outer and inner shafts and pull the handwheel out. Loosen and
remove the large black thumbscrews which secure the microtome to the
chamber. Disconnect the motor wiring. Carefully lift the microtome out of the
chamber. (Note: The microtome weighs approximately 45 pounds.) Before
cleaning the microtome, remove the motor by loosening the set screw that
holds it in place. The microtome can be cleaned with absolute alcohol. If any
water based solutions are used, the microtome must be dried thoroughly using
alcohol or a source of warm dry air.

Note: The microtome must be lubricated after cleaning.

Install the motor, taking care to line up the flat side of the shaft with the set
screw. Place the microtome into the crystat chamber and install according to
the instructions in Section 2.1 Microtome Adjustment. Re-connect the motor
wiring. Replace rubber drain plug.

Periodically, the air flow path for the refrigeration system should be checked
for any restrictions (dust, dirt). Remove the access panel on the left side of the
cryostat and clean out the condenser. Also, ensure that the vents on the right
side are free of dust or dirt.

Minotome Plus Operation Manual 17

4.2 Decontamination
If infectious tissue has been sectioned, IMMEDIATELY decontaminate the
work area and the inside of the microtome cryostat chamber as cited by Billy
L. Swisher and Edwin P. Ewing Jr. in the article entitled, Frozen Sectioning
Techniques for Tissues Infected by the Aids Virus in the Journal of
Histotechnology, Vol. 9, No. 1, March, 1986, page 29:

Specimen Handling
Infections by the AIDS virus are known to occur only by direct contamination of
the blood (1) or mucosal surfaces (2). Therefore, safety measures are directed
toward prevention of cuts, and protection of the eyes, nose and mouth.

Protective Clothing
1. Double gloves
2. Eyeglasses or goggles
3. Mask
4. Gown

Technique
1. Confine possible benchtop contamination to small area.
2. Drop mounting medium onto specimen holder plate and at the
appropriate time add tissue. DO NOT allow dispenser to touch
tissue.
3. DO NOT create any aerosol in the chamber.
4. Briefly immerse entire frozen section slide in 10% formalin.
5. Remove double gloves, withdraw slide from formalin with clean
forceps and proceed with staining procedure.
6. When technical procedure is completed, put on clean gloves and
place residual tissue in 10% formalin to fix thoroughly before it is
handled again.

Decontamination of Cryostat
The AIDS virus is known to be inactivated by 0.5% sodium hypochlorite (1:10
dilution of household bleach) (3,4), 95% ethanol (3, 4, 5, 6) and 10% formalin
(6). Sodium hypochlorite is the most effective decontaminant known for the
virus but it will also corrode metal. Therefore, 95% ethanol is favored for the
inside of the cryostat. The AIDS virus does survive both freezing and drying (4,
6), so decontamination is essential.
1. Turn off refrigeration. Allow chamber to warm to room temperature.
2. With protective clothing in place, remove the blade from microtome
and place it in a pan of 95% ethanol on the floor of the cryostat
chamber where water will collect.
3. If complete defrosting is desired, pour a small amount of 95% ethanol
on the floor of the cryostat chamber where water will collect.
4. Wipe all exposed surfaces inside and outside the cryostat chamber
with a towel or sponge that has been soaked in 95% ethanol.
5. Replace the disposable blade holder or knife from freezer.
6. Turn on refrigeration for return to desired temperature.

Another decontamination technique, recommended before the ethanol

sensitivity of the AIDS virus was known, consists of placing a large beaker of
40% formalin in the closed and defrosting chamber overnight. However, the
moveable parts must be lubricated before the cryostat is used again.

18 Minotome Plus Operation Manual

 Cleanup of Work Area
Personnel not directly involved with preparing frozen section and decontaminat-
ing the cryostat must also be protected from exposure to the AIDS virus.
1. Do not touch other objects in room while wearing contaminated gloves.
2. Place instrument in 0.5% sodium hypochlorite for 15 minutes, wash
well in tap water and autoclave.
3. Wipe bench top with 0.5% sodium hypochlorite.
4. Place all contaminated towels, protective clothing, etc. in double
plastic bags. Use separate containers for disposable and reusable
items. Bags MUST NOT contain sharp objects.
5. Contaminated garments should never be worn outside the frozen
section room.
6. Attach biohazard labels to bags.
7. Wash hands before leaving the area.
4.3 Spare Parts
Part. No. Description
3347 Index Post (18 to 42 microns)
3434 Disposable Blade Holder
H-DMB-HD Disposable Blades (heavy duty)
H-DMBH Disposable Blade Holder
H-LUB Shur/Lube Microtome Lubricating Oil
3331 Specimen Holder (Concentric Circles)
3408 Specimen Holder (Cross Hatch)
H-TFM Tissue Freezing Medium
H-FRZ-1 Shur/Freeze Cryogen Spray
3267 Anti-roll Plate with Feed Screw
3267A Anti-roll Plate without Feed Screw
H-ARD Anti-roll Device
3330 Ball Chuck for specimen holders #3331 and #3408
3375 Replacement Anti-roll Assembly
2085 Heat Extractor
H-BR Brushes; Camel Hair; 1/8", 1/4", 3/8" wide bristles
4.4 Lubrication
Lubrication of the microtome is important to maintain smooth, trouble-free
sectioning. The microtome should be lubricated regularly based on usage.
(For example, if the microtome is used daily, lubricate it weekly. If the
TM
microtome is used weekly, lubricate it monthly. Shur/Lube has a proper
blend of lubricants to protect the microtome.

The microtome components which require lubrication are shown in the

following diagrams. They include: the inner shaft bearings (1), the blade
holder assembly sliding surfaces (2), the dovetail upright gibs (3), the main
feed screw and sliding block (4).

When lubricating the bearings (1), the dimples must first be pressed down to
allow oil to flow into the bearings. A broken paperclip is ideal for the purpose.

When lubricating the blade holder sliding surfaces (2), loosen the locking lever
and slide the assembly out. Place one drop on either side of the track, slide
the assembly back in, and tighten the locking lever.

Minotome Plus Operation Manual 19

 OIL (1)

OIL (2)

OIL (3)

OIL (3)

OIL (4)

20 Minotome Plus Operation Manual

 When lubricating the upright gibs, position the handwheel so that the specimen
holder assembly is at its lowest height. Place a couple of drops on either side
of each dovetail and allow it to run down the upright gib.

When lubricating the apertures in the feed screw and sliding block (4), put a
small paperclip or pin into each hole. Drip oil down it into the aperture. Place
a few drops on the horizontal sliding surfaces.

4.5 Warranty
TRIANGLE BIOMEDICAL SCIENCES, INC. (TBS), for itself and all its
subsidiaries, does hereby warrant for a period of one year (12 months) from the
date of shipping, under normal and proper usage, all of its products against
defects in workmanship and material, and will repair or replace the same
without charge when shipped prepaid to TBS's factory in Durham, NC, USA.
If the product cannot be so shipped (except for parts and accessories), there
will be a charge for all expenses incurred for repairing or replacing the product
on the user's premises.

Parts and accessories manufactured or supplied by others are warranted only

under the regular warranty of the manufacturer or supplier of such parts in
so far as TBS or its subsidiaries are able to transfer the benefit of such
warranties to the user.

This warranty supersedes and is given in lieu of all implied warranties, and is
void if user does not provide unit with continuous ample electrical power at
constant voltage, consistent with the specifications of the product.

User is required to return the warranty card to TBS within ten (10) days after
receipt of the product in order to validate the warranty.

4.6 Condition of Returned Equipment

Before returning equipment to TBS, you must contact TBS or your dealer and
receive a return goods authorization. All returned units must be
decontaminated, free of radioactivity, and free of hazardous and
infectious materials. The RGA paperwork includes a Certificate of
Decontamination for you to sign indicating that you have performed these
steps. TBS will not accept the shipment until this signed certificate is
received.

You must prepay transportation to the service depot. In addition, TBS must
receive unit packed and shipped in compliance with our shipping/crating
instructions. (These can be faxed upon request.) Failure to properly crate
cryostat and ship with handwheel locked, could result in additional repair fees.

Minotome Plus Operation Manual 21

 5.0 SPECIFICATIONS
Microtome
Type Minot® , rotary
Section Thickness 2 to 16 microns - standard
18 to 42 microns - optional
Increments two microns

Cryostat
Temperature Control Range Ambient to -30°C (@ 20°C ambient)
Increments 1°C

Cool Down Ambient to -20°C in 60 minutes

(@ 20°C ambient)

Quick Freeze Temperature Approximately -40°C

Refrigerant Refrigerant type shown on dataplate

Defrost Manual or Automatic

Heat Output (typical) 2000 BTU/hr.

Power Reqirements
Cat. No.s 2563 & 2574 115V ±10%/60 Hz/single phase/15A
100V ±10%/50 Hz/single phase/15A
Cat. No.s 2564 & 2575 200-240V ±10%/50 Hz/single phase/7.5A

Dimensions
Height 114 cm (45 in.)
Width 65 cm (25.5 in.)
Depth 69 cm (27 in.)

Packaging Dimensions
Height 142 cm (56 in.)
Width 81 cm (32 in.)
Depth 105 cm (41 in.)

Weight
Net 122 kg (269 lbs.)
Shipping 159 kg (350 lbs.)

Note: Typical sound level emissions do not exceed 70 dB(A).

Specifications Subject To Change Without Notice

22 Minotome Plus Operation Manual

 Temperature Selection Table (In Minus °C)

Type of Tissue 7-10 10-13 13-16 16-20 20-25 25-30

Adipose Tissue
Adrenal
Bladder
Bone Marrow
Brain
Breast, with fat
Breast, without fat
Cartilage
Cervix
Fat
Heart
Intestinal
Kidney
Larynx
Lip
Liver
Lung
Lymph Node
Lymphatic
Lymphoid
Muscle
Nose
Omentum
Ovary
Pancreas
Prostrate
Rectal
Scrapings
Skin, with fat
Skin, without fat
Soft Parenchyma Lesion
Spleen
Testicular
Thyroid
Tongue
Uterine Curettings
Uterus

Minotome Plus Operation Manual 23