Ante Biško, Antonio Čepelak, Sanja Slunjski, Kristijan Konopka & Boris Lazarević

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Chemical composition of black chokeberry (Aronia melanocarpa) leaf,

fruit, juice and juice sediment


Ante Biško1*, Antonio Čepelak2, Sanja Slunjski1, Kristijan Konopka1 & Boris Lazarević1
1University of Zagreb Faculty of Agriculture,Department of Plant Nutrition, Svetošimunska 25, HR-10000 Zagreb, Croatia, *coresponding author, e-mail:[email protected]
2student, University of Zagreb Faculty of Agriculture, Svetošimunska 25, HR-10000 Zagreb, Croatia

1. INTRODUCTION
Black chokeberries (Aronia melanocarpa) are considered to be functional food containing high amounts of anthocyanins, phenols, antioxidants, vitamins and minerals.
Whereas organic compounds are well studied, there is little research on the mineral composition of the chokeberries. Thus, the presented study is focused on the
determination of N, P, K, Ca, Mg, Fe, Zn, Mn, Cu and B. Leaves, fruits, juice and juice sediment of black chokeberry (Aronia melanocarpa, cv. Nero) were analysed for
the content of macroelements, % of DW (N, P, K, Ca, Mg) and microelements, mg/kg in DW (Fe, Zn, Mn, Cu and B).

2. MATERIALS AND METHODS


Tab. 1 Results of basic chemical analyses of soil The platation was established in November 2017 (2,5ha) with
Analytical Sample two-year-old seedlings, and the drip irrigation system was
number mark pH % AL-mg/100g installed. The soil in the plantation is moderately supplied
H2O nKCl humus N P2O5 K2O with nutrients and organic matter (tab.1), and moderately
1172101 0 - 30 cm 6,67 5,45 2,81 0,13 12,8 30,5
suitable (powdery loam, PL) in terms of mechanical
1172102 30 - 60 cm 6,65 5,27 1,72 0,08 4,8 21,0
composition (tab.2). The planting distance was 4.0*0.9m
Tab. 2. Results of mechanical soil analysis (suitable for mechanical harvesting). The space between the
(mechanical composition of soil in Na-pyrophosphate) rows is grassed.
% content of particles, diameter in mm
Depending on the sample (leaf, fruit, juice, sediment),
Analytical Sample Coarse Fine Coarse Fine Texture
number mark sand sand powder powder
Clay mark samples were taken in 2020, 2021, and 2022, and analyzed in
2,0-0,2 0,2-0,063 0,063-0,02 0,02-0,002 <0,002 accordance with the AOAC protocol (2015), Official Methods
PL of Analysis. Association of Official Analytical Chemists.
1172101 0-30 cm 2,2 7,8 37,6 35,7 16,7 Powdery loam

A computer programe Excel 7.0 was used for data analaysis


PL
1172102 30-60cm 1,0 6,4 37,1 33,0 22,5 Powdery loam and to do the graphic design. Figs.1&2 Aronia plantation (IV/18)&mechanical harvesting (VIII/20)

3. RESULTS AND DISCUSSION


In the leaf, the most abundant macroelement is Ca, and the most abundant microelement is Fe, while in the fruit the most abundant macroelement is K, and
the most abundant microelement is B.
Figs. 3&4 Macroelements and microelements in leaf and fruit; Fig.5 Ratio of leaf/fruit macroelements (%/DW) and leaf/fruit microelements (mg/kg DW)

Average values of macroelements and microelements in the juice were, mg/L: 1057.3(N), 267.9(P) , 3407.4(K), 242.4(Ca), 200.3(Mg), and: 0.89(Fe), 0.39(Zn),
2.34(Mn), 0.07(Cu), 0.51(B), fig.6; and in the juice sediment, % of DM: 0.70(N), 0.03(P), 1.35(K), 0.04(Ca), 0.07(Mg), and: mg/kg DM: 94.24(Fe), 1.25(Zn),
9.79(Mn), 14.42(Cu) and 3.31(B), fig. 7.
Figs. 6&7 Average values of macroelements and microelements in the juice (mg/L) and j. sediment: macroelements (%DW) and microelements (mg/kg DW)

4. CONCLUSION 5. REFERENCES
The concentrations of individual elements depended on the sample (leaf, fruit, 1. AOAC (2015), Official Methods of Analysis. Association of Official Analytical Chemists. 18th Edition, AOAC, Arlington, 806-814.
https://www.scirp.org/(S(czeh2tfqw2orz553k1w0r45))/reference/referencespapers.aspx?referenceid=2913459–pristupljeno 22.5.2023.
juice or juice sediment). Ca is the most abundand in the leaf, and K in the fruit.
2. Bussières, J., Boudreau, S., Clément–Mathieu, G., Dansereau, B., iRochefort, L. (2008). Growing Black Chokeberry (Aroniamelanocarpa)
Fe is the most abundant in the leaf and B in the fruit. In relation to the same in Cut-over Peatlands, HortScience, 43(2), 494-499. Retrieved Aug 23, 2021,
values in the leaf, K and Cu accumulated the best, and Ca and Mn the worst in https://journals.ashs.org/hortsci/view/journals/hortsci/43/2/article-p494.xml

the fruit. 3. Juranović Cindrić, Iva; Zeiner, Michaela; Mihajlov-Konanov, Darija; Stingeder, Gerhard (2017) Inorganic Macro- andMicronutrients in
“Superberries” Black Chokeberries (Aronia melanocarpa) and Related Teas // International Journal of Environmental Research and
Potassium is the most abundant macroelement both in the juice and in the Public Health, 14 (2017), 5; 539-549

sediment. As for the microelements, Mn is the most abundant in the juice and 4. Ochmian, I. D., Grajkowski, J. Smolik, M. (2012). Comparisonof some morphological features, quality and chemical content off our
Fe in the sediment. Significant sedimentation was determined in Cu, while Mn cultivars of chokeberry fruits (Aronia melanocarpa). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(1), 253-260.
https://doi.org/10.15835/nbha4017181
and especially B and Zn had significantly lower values in the sediment.
5. Škorić A. (1986) Priručnik za pedološka istraživanja, Zagreb

V Balkan Symposium on Fruit Growing, Zagreb, Croatia, June 18-21, 2023

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