Data Communication Protocol for

MEK-7222J/K Automated
Hematology Analyzer

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on this manual, please contact us at:
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Technical Reference Manual MEK-7222J/K L.1

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L.2 Technical Reference Manual MEK-7222J/K

 Contents
RS-232C Data Transfer ....................................................................................................... 1
General ..................................................................................................................... 1
Wiring (conforms to RS-232C specifications) ................................................. 1
RS232C Specifications ................................................................................... 1
WA-460V Settings .......................................................................................... 1
Transfer Format ........................................................................................................ 2
Numeric Data Transfer Format ....................................................................... 2
Transfer Format for PC ................................................................................... 3
Distinction of the External Instruments ........................................................... 3
Transfer Format Example ............................................................................... 4
Socket Pin Assignment ..................................................................................................... 17
Serial Port 1 ............................................................................................................ 17
Bar Code Socket (Serial Port 2) ............................................................................. 17
Printer Socket ......................................................................................................... 18
USB Socket ............................................................................................................ 18

Technical Reference Manual MEK-7222J/K C.1

 RS-232C Data Transfer

General
Sample data can be transferred to the optional printer or a personal computer via
the serial ports on the rear panel of the hematology analyzer. This allows you to
print or search specific data or perform the statistical work. Sample data can be
automatically transferred after each count.

CAUTION CAUTION
• In order to avoid any safety hazard, only Connect only the specified instrument to the
connect personal computers which are hematology analyzer and follow the specified
approved by IEC 60950 procedure. Failure to follow this instruction may
• Only use the specified power cord for the PC. result in electrical shock or injury to the operator,
and cause fire or instrument malfunction.

NOTE
• To transfer the sample data to a personal computer, the data transfer
settings on the personal computer must be set to match the data
transfer conditions of this hematology analyzer beforehand.
• To transfer data to a personal computer, set SERIAL PORT 1 or 2 to
ON on the OUTPUT KEY screen, or AUTO on the AUTO OUTPUT
screen. Refer to Section 3 of the MEK-7222J/K Operator’s Manual.

Wiring (conforms to RS-232C specifications)

MEK-7222K Personal computer or card reader MEK-7222K Personal computer or card reader
(25 pin D sub connector) (25 pin D sub connector) (25 pin D sub connector) (9 pin D sub connector)
TXD 2 2 TXD TXD 2 2 RXD
RXD 3 3 RXD RXD 3 3 TXD
RTS 4 4 RTS RTS 4 4 DTR
CTS 5 5 CTS CTS 5 5 GND
DSR 6 6 DSR DSR 6 6 DSR
GND 7 7 GND GND 7 7 RTS
DTR 20 20 DTR DTR 20 8 CTS

Connector Connector Connector Connector

housing housing housing housing

RS232C Specifications
Baud rate: 1200, 2400, 4800, 9600, 19200 bps
Data bit length: 7, 8 bits
Parity check: No parity, Odd parity, Even parity
Stop bit length: 1, 2 bits

WA-460V Settings
Baud rate: 9600 bps
Data bit length: 8 bits
Parity check: Even parity
Stop bit length: 1 bit

Technical Reference Manual MEK-7222J/K 1

Transfer Format
Numeric Data Transfer Format
You can select transfer format on the COMMUNICATION screen. There are
three formats for transferring data: WA-460V, PC, and OTHER. The default
setting is WA- 460V. When connecting a personal computer to the serial port,
select PC. For details, refer to Section 3 of the MEK-7222J/K Operator’s
Manual.

You can also select to transfer the data for CBC parameters only or all 22
parameters. An ID can be either 4 or 13 characters.

Following are examples of each transfer format.

WA-460V
• 0D (CR: Carriage Return) and 0A (LF: Line Feed) are used.
• ASCII code
• Transferring order
1. Date
2. ID
3. Parameter data
WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT, NE%, LY%, MO%,
EO%, BA%, NE, LY, MO, EO, BA, RDW, PCT, MPV, PDW
In this format, scattergrams and histograms cannot be transferred.
Data volume (bytes) depends on the date setting on the COMMUNICATION
screen and the card printer settings on the CARD PRINTER screen.

PC
• Data transfer starts from 02 (STX: Start of text) and ends with 03 (ETX: End
of text)
• Each data separated by 0D (CR: Carriage Return)
• ASCII code
• Transferring order
Common data block
• Hematology analyzer information
• Measurement data
• Flag data
Transfer format for a personal computer is fixed. Before transferring the data,
adjust the setting of the receiver to match this format.

2 Technical Reference Manual MEK-7222J/K

 Transfer Format for PC
When you set the output format to “PC” and the software version of the
automated hematology analyzer is V04-01 or later, you can choose from 2
transfer formats.
• Transfer format V03-01 has flag data of SNC and PRI, expanded data, etc.
• When the software version of the analyzer is V03-03 or before, transfer format
is fixed to V02-03.
The relation of the software version and transfer format version is as follows.
The default setting is underlined.

Software version of Analysis version Transfer format version

hematology analyzer
V03-03 or before none V02-03 (Fixed)
V04-01 or later V03-01 V02-03 or V03-01
The transfer format can be changed in the
COMMUNICATION screen. For details, refer to
Section 3 “Changing Settings” of the operator’s
manual.

Distinction of the External Instruments

When connecting more than one instrument, you can distinct them as follows.
• Transfer format V02-03:
You can distinct the instruments by COM port No.
• Transfer format V03-01:
– When connecting two instruments.
Set the Unit No. in the ANALYZER NAME - USB SETTING - OUTPUT
FORMAT in the System Settings. For details, refer to the Section 3
“Changing Settings” of the operator’s manual.
– When connecting three or more instruments.
You can distinct the instruments by COM port No.

Technical Reference Manual MEK-7222J/K 3

Transfer Format Example

WA-460V
Transferred Data (In Characters) Transferred Data (In Hexadecimal)
<ESC 'C' '4' '0'> 1B 63 34 30
<ESC '2'> WA-460V 1B 32
<ESC 'd' 5> Control codes 1B 64 5
<ESC 't' 0> 1B 74 0

03/ 1/10 (Date) 20 20 30 33 2F 20 31 2F 31 30 0D 0A

0001 (ID) 20 20 20 20 20 20 20 20 20 30 30 30 31 0D 0A
7.5 (WBC) 20 20 20 37 2E 35 20 20 0D 0A
4.45 (RBC) 20 20 34 2E 34 35 20 20 0D 0A
13.6 (HGB) 20 20 31 33 2E 36 20 20 0D 0A
40.2 (HCT) 20 20 34 30 2E 32 20 20 0D 0A
90.1 (MCV) 20 20 39 30 2E 31 20 20 0D 0A
30.6 (MCH) 20 20 33 30 2E 36 20 20 0D 0A
33.8 (MCHC) 20 20 33 33 2E 38 20 20 0D 0A
273 (PLT) 20 20 20 32 37 33 20 20 0D 0A
<LF> 0D 0A
60.0 % NE% (NE%) 20 20 36 30 2E 30 20 20 25 20 20 20 20 20 4E 45 25 0D 0A
30.5 % LY% (LY%) 20 20 33 30 2E 35 20 20 25 20 20 20 20 20 4C 59 25 0D 0A
5.0 % MO% (MO%) 20 20 20 35 2E 30 20 20 25 20 20 20 20 20 4D 4F 25 0D 0A
3.8 % EO% (EO%) 20 20 20 33 2E 38 20 20 25 20 20 20 20 20 45 4F 25 0D 0A
0.7 % BA% (BA%) 20 20 20 30 2E 37 20 20 25 20 20 20 20 20 42 41 25 0D 0A
4.4 x1000 NE (NE) 20 20 20 34 2E 34 20 20 78 31 30 30 30 20 4E 45 0D 0A
2.3 x1000 LY (LY) 20 20 20 32 2E 33 20 20 78 31 30 30 30 20 4C 59 0D 0A
0.4 x1000 MO (MO) 20 20 20 30 2E 34 20 20 78 31 30 30 30 20 4D 4F 0D 0A
0.3 x1000 EO (EO) 20 20 20 30 2E 33 20 20 78 31 30 30 30 20 45 4F 0D 0A
0.1 x1000 BA (BA) 20 20 20 30 2E 31 20 20 78 31 30 30 30 20 42 41 0D 0A
<LF> 0D 0A
13.4 % RDW (RDW) 20 20 31 33 2E 34 20 20 25 20 20 20 20 20 52 44 57 0D 0A
0.21 % PCT (PCT) 20 20 30 2E 32 31 20 20 25 20 20 20 20 20 50 43 54 0D 0A
7.6 fL MPV (MPV) 20 20 20 37 2E 36 20 20 66 4C 20 20 20 20 4D 50 56 0D 0A
17.9 % PDW (PDW) 20 20 31 37 2E 39 20 20 25 20 20 20 20 20 50 44 57 0D 0A

<ESC 'F' 1> 1B 46 1

WA-460V
0C
Control codes
<ESC 'q'> 1B 71

4 Technical Reference Manual MEK-7222J/K

PC (transfer format V03-01)
<Common data block>
No. of Transferred Data in Characters
Transfer Items Example Transferred Data in Hexadecimal
Byte
<Start> Common data block
Start of Text 0x02 1 STX 02
Hematology analyzer information
Type “MEK-7222” 11 M E K - 7 2 2 2 CR 4D 45 4B 2D 37 32 32 32 20 20 0D
Parameter no “22” 6 2 2 CR 20 20 20 32 32 0D
Send data bytes “01024” 6 0 1 0 2 4 CR 30 31 30 32 34 0D
Sampling mode “MANUAL” 13 M A N U A L CR 4D 41 4E 55 41 4C 20 20 20 20 20 20 0D
Parameter “CBC + Diff” 13 C B C + D i f f CR 43 42 43 20 2B 20 44 69 66 66 20 20 0D
Sample code “01” 3 0 1 CR 30 31 0D
Sample label “BLOOD” 17 B L O O D CR 42 4C 4F 4F 44 20 20 20 20 20 20 20 20 20 20
20 0D
Rack location “MMM” 5 M M M CR 4D 4D 4D 20 0D
Seq# “0000001” 11 0 0 0 0 0 0 1 CR 30 30 30 30 30 30 31 20 20 20 0D
Software version “V04-02” 9 V 0 4 - 0 2 CR 56 30 34 2D 30 32 20 20 0D
Analysis program version “V03-02” 9 V 0 3 - 0 2 CR 56 30 33 2D 30 32 20 20 0D
Format version “V03-01” 9 V 0 3 - 0 1 CR 56 30 33 2D 30 31 20 20 0D
Total data bytes “01536” 6 0 1 5 3 6 CR 30 31 35 33 36 0D
Data block pattern “1” 6 1 CR 31 20 20 20 20 0D
Reserve data 4 SP × 3 Byte + CR × 1 Byte 20 × 3 Byte + 0D × 1 Byte
Measurement data
Date “20050101” 17 2 0 0 5 CR 0 1 CR 0 1 CR CR 32 30 30 35 0D 30 31 0D 30 31 0D 20 20 20 20
20 0D
Time “153000” 9 1 5 CR 3 0 CR 0 0 CR 31 35 0D 33 30 0D 30 30 0D
ID “ABCDEFGH:0001” 16 A B C D E F G H : 0 0 0 1 CR 41 42 43 44 45 46 47 48 3A 30 30 30 31 20 20 0D
WBC “6.2” 7 6 . 2 CR 20 36 2E 32 20 20 0D
NE% “70.6” 7 7 0 . 6 CR 37 30 2E 36 20 20 0D
LY% “21.2” 7 2 1 . 2 CR 32 31 2E 32 20 20 0D
MO% “2.5” 7 2 . 5 CR 20 32 2E 35 20 20 0D
EO% “5.4H” 7 5 . 4 H CR 20 35 2E 34 48 20 0D
BA% “0.3” 7 0 . 3 CR 20 30 2E 33 20 20 0D
NE “4.4” 7 4 . 4 CR 20 34 2E 34 20 20 0D
LY “1.3” 7 1 . 3 CR 20 31 2E 33 20 20 0D
MO “0.2” 7 0 . 2 CR 20 30 2E 32 20 20 0D
EO “0.2” 7 0 . 2 CR 20 30 2E 32 20 20 0D
BA “0.0” 7 0 . 0 CR 20 30 2E 30 20 20 0D
RBC “5.10” 7 5 . 1 0 CR 35 2E 31 30 20 20 0D
HGB “14.4” 7 1 4 . 4 CR 31 34 2E 34 20 20 0D
HCT “42.3” 7 4 2 . 3 CR 34 32 2E 33 20 20 0D
MCV “86.2” 7 8 6 . 2 CR 38 36 2E 32 20 20 0D
MCH “28.5” 7 2 8 . 5 CR 32 38 2E 35 20 20 0D
MCHC “33.1” 7 3 3 . 1 CR 33 33 2E 31 20 20 0D
RDW “11.5” 7 1 1 . 5 CR 31 31 2E 35 20 20 0D
PLT “280” 7 2 8 0 CR 20 32 38 30 20 20 0D
PCT “0.15” 7 0 . 1 5 CR 30 2E 31 35 20 20 0D
MPV “7.2” 7 7 . 2 CR 20 37 2E 32 20 20 0D
PDW “18.5” 7 1 8 . 5 CR 31 38 2E 35 20 20 0D
Reserve data 210 SP × 209 Byte + CR × 1 Byte 20 × 209 Byte + 0D × 1 Byte
Flag data (with flag +, without flag (space)) (ex. 20 0D)
Leukocytosis “+” 2 + CR 2B 0D
Leukopenia “+” 2 + CR 2B 0D
Neutrophilia “+” 2 + CR 2B 0D
Neutropenia “+” 2 + CR 2B 0D
Lymphocytosis “+” 2 + CR 2B 0D
Lymphopenia “+” 2 + CR 2B 0D
Monocytosis “+” 2 + CR 2B 0D
Eosinophilia “+” 2 + CR 2B 0D
Basophilia “+” 2 + CR 2B 0D
Blasts “+” 2 + CR 2B 0D
Immature Granulocyte “+” 2 + CR 2B 0D
Left Shift “+” 2 + CR 2B 0D
Atypical lymphocytes “+” 2 + CR 2B 0D
Poor hemolyzation “+” 2 + CR 2B 0D
Small nucleated cell “+” 2 + CR 2B 0D
Ly-Mo interference “+” 2 + CR 2B 0D
Ne-Eo interference “+” 2 + CR 2B 0D
Reserve data 14 SP × 13 Byte + CR × 1 Byte 20 × 13 Byte + 0D × 1 Byte
Erythrocytosis “+” 2 + CR 2B 0D
Anemia “+” 2 + CR 2B 0D
Anisocytosis “+” 2 + CR 2B 0D
Microcytosis “+” 2 + CR 2B 0D
Macrocytosis “+” 2 + CR 2B 0D
Hypochromia “+” 2 + CR 2B 0D
Abnormal MCHC “+” 2 + CR 2B 0D
Reserve data 10 SP × 9 Byte + CR × 1 Byte 20 × 9 Byte + 0D × 1 Byte
Thrombocytosis “+” 2 + CR 2B 0D
Thrombocytopenia “+” 2 + CR 2B 0D
PLT Clumps “+” 2 + CR 2B 0D
PLT-RBC interference “+” 2 + CR 2B 0D
Reserve data 8 SP × 7 Byte + CR × 1 Byte 20 × 7 Byte + 0D × 1 Byte
Reserve data
Reserve data 400 SP × 399 Byte + CR × 1 Byte 20 × 399 Byte + 0D × 1 Byte
<End> Common data block
End of Text 0x03 1 ETX 03

Technical Reference Manual MEK-7222J/K 5

<Extended data block>
No. of
Transfer Items Example Transferred Data in Characters Transferred Data in Hexadecimal
Byte
<Start> Extended data block
Start of Text 0x02 1 STX 02
Hematology analyzer information
Identifier “EXP” 4 E X P CR 45 58 50 0D
Send data bytes “00512” 6 0 0 5 1 2 CR 30 30 35 31 32 0D
Type “MEK-7222” 11 M E K - 7 2 2 2 CR 4D 45 4B 2D 38 32 32 32 20 20 0D
Unit no “1” 3 1 CR 20 31 0D
Work list data
Name “DAVID” 27 DA V I D ... … CR 44 41 56 49 44 … … 20 20 20 20 … … 0D
Sex “MALE” 7 MA L E CR 4D 41 4C 45 20 20 0D
Date of birth “19800219” 11 19 8 0 CR 0 2 CR 1 9 CR 31 39 38 30 0D 30 32 0D 31 39 0D
Age “22” 4 2 2 CR 20 32 32 0D
Department “INTERNAL” 14 I N T E R N A L CR 49 4E 54 45 52 4E 41 4C 20 20 20 20 20 0D
Physician “WATSON” 27 W A T S O N ... ... CR 57 57 41 54 53 4F … … 20 20 20 … … 0D
Operator name “” 9 CR 20 20 20 20 20 20 20 20 0D
Comments “No problem.” 129 N o p r o b l e m . ... 4E 4E 6F 20 70 72 6F 62 6C 65 6D
... CR … … 20 20 20 20 20 20 … … 0D
Normal range table no “0” 2 0 CR 30 0D
Work list flag “1” 2 1 CR 31 0D
Control mode flag “” 2 CR 20 0D
Reserve data for work list data 32 SP × 31 Byte + CR × 1 Byte 20 × 31 Byte + 0D × 1 Byte
Normal range setting data
WBC-LOW “4.0” 5 4 . 0 CR 20 34 2E 30 0D
WBC-HIGH “9.0” 5 9 . 0 CR 20 39 2E 30 0D
NE%-LOW “42.0” 5 4 2 . 0 CR 34 32 2E 30 0D
NE%-HIGH “85.0” 5 8 5 . 0 CR 38 35 2E 30 0D
LY%-LOW “11.0” 5 1 1 . 0 CR 31 31 2E 30 0D
LY%-HIGH “49.0” 5 4 9 . 0 CR 34 39 2E 30 0D
MO%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
MO%-HIGH “9.0” 5 9 . 0 CR 20 39 2E 30 0D
EO%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
EO%-HIGH “3.0” 5 3 . 0 CR 20 33 2E 30 0D
BA%-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
BA%-HIGH “2.0” 5 2 . 0 CR 20 32 2E 30 0D
NE-LOW “1.7” 5 1 . 7 CR 20 31 2E 37 0D
NE-HIGH “7.7” 5 7 . 7 CR 20 37 2E 37 0D
LY-LOW “0.4” 5 0 . 4 CR 20 30 2E 34 0D
LY-HIGH “4.4” 5 4 . 4 CR 20 34 2E 34 0D
MO-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
MO-HIGH “0.8” 5 0 . 8 CR 20 30 2E 38 0D
EO-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
EO-HIGH “0.3” 5 0 . 3 CR 20 30 2E 33 0D
BA-LOW “0.0” 5 0 . 0 CR 20 30 2E 30 0D
BA-HIGH “0.2” 5 0 . 2 CR 20 30 2E 32 0D
RBC-LOW “3.80” 5 3 . 8 0 CR 33 2E 38 30 0D
RBC-HIGH “5.30” 5 5 . 3 0 CR 35 2E 33 30 0D
HGB-LOW “11.0” 5 1 1 . 0 CR 31 31 2E 30 0D
HGB-HIGH “17.0” 5 1 7 . 0 CR 31 37 2E 30 0D
HCT-LOW “36.0” 5 3 6 . 0 CR 33 36 2E 30 0D
HCT-HIGH “56.0” 5 5 6 . 0 CR 35 36 2E 30 0D
MCV-LOW “80.0” 5 8 0 . 0 CR 38 30 2E 30 0D
MCV-HIGH “100” 5 1 0 0 CR 20 31 30 30 0D
MCH-LOW “28.0” 5 2 8 . 0 CR 32 38 2E 30 0D
MCH-HIGH “36.0” 5 3 6 . 0 CR 33 36 2E 30 0D
MCHC-LOW “31.0” 5 3 1 . 0 CR 33 31 2E 30 0D
MCHC-HIGH “37.0” 5 3 7 . 0 CR 33 37 2E 30 0D
RDW-LOW “11.5” 5 1 1 . 5 CR 31 31 2E 35 0D
RDW-HIGH “16.5” 5 1 6 . 5 CR 31 36 2E 35 0D
PLT-LOW “120” 5 1 2 0 CR 20 31 32 30 0D
PLT-HIGH “380” 5 3 8 0 CR 20 33 38 30 0D
PCT-LOW “0.10” 5 0 . 1 0 CR 30 2E 31 30 0D
PCT-HIGH “1.00” 5 1 . 0 0 CR 31 2E 30 30 0D
MPV-LOW “5.0” 5 5 . 0 CR 20 35 2E 30 0D
MPV-HIGH “10.0” 5 1 0 . 0 CR 31 30 2E 30 0D
PDW-LOW “12.0” 5 1 2 . 0 CR 31 32 2E 30 0D
PDW-HIGH “18.0” 5 1 8 . 0 CR 31 38 2E 30 0D
<End> Extended data block
End of Text 0x03 1 ETX 03

• “0x0D” is at the end of each item as separating character.

• Each item is entered by ASCII code. Only “Start of Text” and “ End of Text” are entered by control code.
• Each item is initialized at space (0x20). Unused items are also initialized at space and have “0x0D” in the end.

6 Technical Reference Manual MEK-7222J/K

Transferring Item Description
<Common data block>

Item No. Item No. of Bytes Description

Hematology analyzer information
1 Type 11 Sets the model of the hematology analyzer “MEK-7222”.
2 Parameter No. 6 Sets the number of measurable parameters “22”.
3 Send data bytes 6 Set the total number of transferring data “01024”.
Sets the sampling method.
Normal mode: “MANUAL”
High dilution mode 1: “HIGH (M)”
High dilution mode 2: “HIGHER (M)”
Pre-dilution mode (20 μL): “PreDIL 20”
4 Sampling mode 13
Pre-dilution mode (10 μL): “PreDIL 10”
Closed mode with cap pierce unit: “CLOSED”
Open mode with cap pierce unit: “MANUAL”
Closed high dilution mode 1: “HIGH (C)”
Closed high dilution mode 2: “HIGHER (C)”
Sets the measuring parameters.
5 Parameter 13 When measuring 22: CBC + Diff
When measuring 8: CBC
Sets the code for sample type.
When measuring hematology control, “00” is set. When measuring
the other sample, code for each sample is set.
<Default sample code>
Sample Code Sample Code
6 Sample code 3 Control 00 SET 3 03
Blood 01 SET 4 04
SET 2 02 SET 5 05

You can change blood or SET 2 to SET 5 sample name. For

details, refer to operator’s manual.
Sets the sample label.
When measuring the sample, the sample name for each sample is
set.
7 Sample label 17 Default setting is “Control”, “Blood”, “SET 2”, “SET 3”, “SET 4”
and “SET 5”. You can change the name other than “Control”. For
details, refer to the operator’s manual.
When measuring by X-R or L & J program, “Control” is set.
Sets the rack ID and rack position.
1 Bytes: rack ID:
2 to 3 bytes: rack position
8 Rack location 5
4 bytes: space (0x20)
5 bytes: CR (0x0D)
“01” is set in closed mode. “MMM” is set in other mode.
9 Seq# 11 Sets the sequence number.
10 Software version 9 Sets the software version.
Analysis program
11 9 Sets the analysis program version.
version
12 Format version 9 Sets the RS-232C output format version.
Sets the all sent data size “01536” including common data block
13 Total data bytes 6
and extended data block.
Sets the data block that is send after common data block. When the
14 Data clock pattern 6
extended data block is send, “1” is set.
15 Reserve data 4 Sets space (0x20) × 3 + CR (0x0D)..

Technical Reference Manual MEK-7222J/K 7

Measurement data
Sets date. Year, month and day are separated by CR (0x0D). A day
1 Date 17
of the week is not set. Space (0x20) is set.
2 Time 9 Sets time. Hour, minute and second are separated by CR (0x0D).
3 ID 16 Sets the sample ID number.
Sets as follows.
1 to 4 bytes: measurement value
when over the limits: “OVER”
4 WBC to PDW 7 each
when measurement is impossible: space (0x20)
5 to 6 bytes: abnormal value mark
7 bytes: CR (0x0D)
5 Reserve data 210 Sets space (0x20) × 209 + CR (0x0D).
Flag data
Leukocytosis to Ne-Eo
1 2 each Sets “+” when there is a flag, space (0x20) when there is no flag.
interference
2 Reserve data 14 Sets space (0x20) × 13 + CR (0x0D).
Erythrocytosis to
3 2 each Sets “+” when there is a flag, space (0x20) when there is no flag.
abnormal MCHC
4 Reserve data 10 Sets space (0x20) × 9 + CR (0x0D).
Thrombocytosis to
5 2 each Sets “+” when there is a flag, space (0x20) when there is no flag.
PLT-RBC interference
6 Reserve data 8 Sets space (0x20) × 7 + CR (0x0D).
Reserved data
1 Reserve data 400 Sets space (0x20) × 399 + CR (0x0D).

NOTE
In the reserved data, new items by character codes may be set when the software is upgraded in the future.

8 Technical Reference Manual MEK-7222J/K

<Extended data block>

Item No. Item No. of Bytes Description

Hematology analyzer information
1 Identifier 4 Sets the data block identifier “EXP”.
2 Send data bytes 6 Sets the total number of transferred extended data block “00512”.
3 Type 11 Sets the model of the hematology analyzer “MEK-7222”.
Sets the analyzer name.
4 Unit No. 3 When set to “UNIT 1”: 1
When set to “UNIT 2”: 2
Work list data
Sets the patient name. When not using the work list, a space (0x20)
1 Name 27
× 26 + CR (0x0D) is set.
Sets the patient sex, MALE or FEMALE. When not using the work
2 Sex 7
list, a space (0x20) × 6 + CR (0x0D) is set.
Sets the patient date of birth. When not using the work list, a space
3 Date of birth 11
(0x20) × 10 + CR (0x0D) is set.
Sets the patient age. When not using the work list, a space (0x20)
4 Age 4
× 3 + CR (0x0D) is set.
Sets the department of the patient. When not using the work list, a
5 Department 14
space (0x20) × 13 + CR (0x0D) is set.
Sets the name of the physician. When not using the work list, a
6 Physician 27
space (0x20) × 26 + CR (0x0D) is set.
7 Operator name 9 Not used. Sets space (0x20) × 8 + CR (0x0D).
Sets the comment. When not using the work list, a space (0x20) ×
8 Comments 129
128 + CR (0x0D) is set.
Sets the code of the sample. When measuring hematology control,
9 Normal range table no. 2 “0” is set. When measuring other sample, code for each sample is
set.
Sets the work list flag.
10 Work list flag 2 When measured using the work list: 1
When measured without work list: 0
11 Control mode flag 2 Not used. Sets space (0x20)+CR (0x0D).
12 Reserve data 32 Sets space (0x20) × 31 + CR (0x0D).
Normal range data
WBC-HIGH to PDW- Sets 4 bytes + CR (0x0D) for normal range data used for
1 5 each
LOW measurement.

Technical Reference Manual MEK-7222J/K 9

Abnormal mark
Abnormal mark shows following error.

Item Mark Description Item Mark Description

All H Over normal range LY ? Room temp high, room temp low or
optical count error
L Under normal range * Blasts, atypical Ly or Ly-Mo interference
WBC ? WBC sample error LY% ? Room temp high, room temp low or
optical count error
! Poor hemolyzation * Blasts, atypical Ly or Ly-Mo interference
C PLT clumps MO ? Optical count error
* Small nucleated cell * Blasts, immature Gr, left shift, atipical Ly
or Ly-Mo interference
RBC ? RBC sample error MO% ? Optical count error
* RBC-PLT interference * Blasts, immature Gr, left shift, atipical Ly
or Ly-Mo interference
HGB ! HGB voltage high EO ? Optical count error
? HGB voltage low * Blasts or Ne-Eo interference
* HGB circuit error or WBC OVER EO% ? Optical count error
MCHC ! RBC sample error BA ? Optical count error
PLT ? RBC sample error * Blasts, immature Gr or left shift
C PLT clumps BA% ? Optical count error
* RBC-PLT interference * Blasts, immature Gr or left shift
NE ? Room temp high, room temp low or
optical count error
* Blasts, immature Gr, left shift or Ne-Eo
interference
NE% ? Room temp high, room temp low or
optical count error
* Blasts, immature Gr, left shift or Ne-Eo
interference

Data indication
Data indication (alarm/flag) and their description is as follows.

Classification Description
1 Data cannot be analyzed Data cannot be analyzed.
2 Measurement alarm (data not displayed) Error found during measurement.
3 Measurement alarm (data displayed) Measurement error due to surrounding temperature out of specified range.
Measured data is displayed but measurement accuracy is not reliable.
4 Out of measuring range Out of measuring range.
5 Data with low reliability Abnormal flag detected in the sample. Measurement accuracy is not reliable
due to abnormal cell.
• When WBC flag appears, all WBC parameters are affected by the abnormal
cell. “*” is displayed beside the parameter which is greatly affected.
• When there is possibility of PLT coagulation, “C” is displayed beside the
parameter.
• When there is possibility of poor hemolyzation, “!” is displayed beside the
parameter.
6 Out of normal range Out of normal range setting.

10 Technical Reference Manual MEK-7222J/K

Data, alarm, flag and data transfer

Alarm message/
Classification Data display Symbol display Data transfer
flag display
Data cannot be
1 None None None None
analyzed
Measurement alarm Alarm code no. and Abbreviation of the
2 None None
(data not displayed) message alarm
Measurement alarm “?” beside numeric Alarm code no. and
3 Data displayed Data and identifier
(data displayed) data message
Out of measuring “OVER” message Abbreviation of
4 None None
range displayed “OVER”
Data with low “*”, “!” or “C” beside
5 Data displayed Flag name Data and identifier
reliability numeric data
“H” or “L” beside
6 Out of normal range Data displayed None Data and identifier
numeric data

Abbreviation of the alarm and example of the RS-232C data output format
Example: PLT clumps
WBC LEVEL 1 Transfer code: 0x4C, 0x45, 0x56, 0x45, 0x4C, 0x31, 0x0D

Alarm name Code WBC RBC

WBC fluid level 1 A021 LEVEL 1
WBC fluid level 2 A022 LEVEL 2
WBC fluid level 3 A023 LEVEL 3
WBC bubble 1 A024 BBL 1
WBC bubble 2 A025 BBL 2
WBC bubble 3 A026 BBL 3
WBC bubble 4 A027 BBL 4
WBC clogged A029 CLOG
WBC hardware noise A031 NOISE 2
WBC software noise A032 NOISE 1
RBC fluid level 1 A041 LEVEL 1
RBC fluid level 2 A042 LEVEL 2
RBC fluid level 3 A043 LEVEL 3
RBC bubble 1 A044 BBL 1
RBC bubble 2 A045 BBL 2
RBC bubble 3 A046 BBL 3
RBC bubble 4 A047 BBL 4
RBC clogged A049 CLOG
RBC hardware noise A051 NOISE 2
RBC software noise A052 NOISE 1

Technical Reference Manual MEK-7222J/K 11

Explanation of low reliable indicator and example of RS-232C data output
Example: PLT clumps
WBC 7.3C Transfer code: 0x20, 0x37, 0x2E, 0x33, 0x43, 0x20, 0x0D
PLT 280C Transfer code: 0x20, 0x32, 0x38, 0x30, 0x43, 0x20, 0x0D

Parameters
Flag PCT
Flag NE% LY% MO% EO% BA%
Class. WBC RBC HGB HCT MCHC PLT MPV
NE# LY# MO# EO# BA#
PDW
Blasts * * * * *
Immature
* * *
Granulocyte
Left Shift * * *
Atypical WBC * *
Lymphocytes
Small Nucleated Cell *
Ly-Mo Interference * *
Ne-Eo Interference * *
PLT-RBC
* *
Interference RBC/PLT
PLT Clumps C C C
WBC OVER *
Abnormal MCHC !
Specimen
Poor hemolyzation !

Parameters
Alarm message Code No. NE% LY% MO% EO% BA%
WBC RBC HGB HCT MCHC PLT
NE# LY# MO# EO# BA#
WBC sample error A030 ?
RBC sample error A050 ? ?
HGB voltage low A061 ?
HGB voltage high A062 !
HGB circuit error A063 *
Room temp high A091 ? ?
Room temp low A092 ? ? ?
Optical count error A082 ? ? ? ? ?

12 Technical Reference Manual MEK-7222J/K

PC (transfer format V02-03)

No. of
Transfer Items Example Transferred Data in Characters Transferred Data in Hexadecimal
Byte
<Start> Common data block
Start of Text 0x02 1 STX 02
Hematology analyzer information
Type “MEK-7222” 11 M E K - 7 2 2 2 SP SP CR 4D 45 4B 2D 37 32 32 32 20 20 0D
Parameter no “22” 6 SP SP SP 2 2 CR 20 20 20 32 32 0D
Send data bytes “1024” 6 O 1 0 2 4 CR 30 31 30 32 34 0D
Sample type “VENOUS” 13 V E N O U S SP SP SP SP SP SP CR 56 45 4E 4F 55 53 20 20 20 20 20 20 0D
Parameter “CBC + Diff” 13 C B C + D i f f SP SP CR 43 42 43 20 2B 20 44 69 66 66 20 20 0D
Sample code “00” 3 0 0 CR 30 30 0D
20 20 20 20 20 20 20 20 20 20 20 20
Sample type name “” 17 SP SP SP SP SP SP SP SP SP SP SP SP SP SP
20 20
SP SP CR 20 20 0D
Rack location “01” 5 0 1 SP SP CR 30 31 20 20 0D
Seq# “0000001” 11 0 0 0 0 0 0 1 SP SP SP CR 30 30 30 30 30 30 31 20 20 20 0D
Reserve data 43 SP × 43 Byte 20 × 43 Byte
Measurement data
Date “20020725” 17 2 0 0 2 CR 0 7 CR 2 5 CR SP SP SP 32 30 30 32 0D 30 37 0D 32 35 0D 20 20 20
SP SP CR 20 20 0D
Time “153000” 9 1 5 CR 3 0 CR 0 0 CR 31 35 0D 33 30 0D 30 30 0D
ID “ABCDEFGH:0001” 16 A B C D E F G : 0 0 0 1 SP 41 42 43 44 45 46 47 48 3A 30 30 30 31 20
SP CR 20 0D
WBC “6.2” 7 SP 6 . 2 SP SP CR 20 36 2E 32 20 20 0D
NE% “70.6” 7 7 0 . 6 SP SP CR 37 30 2E 36 20 20 0D
LY% “21.2” 7 2 1 . 2 SP SP CR 32 31 2E 32 20 20 0D
MO% “2.5” 7 SP 2 . 5 SP SP CR 20 32 2E 35 20 20 0D
EO% “5.4H” 7 SP 5 . 4 H SP CR 20 35 2E 34 48 20 0D
BA% “0.3” 7 SP 0 . 3 SP SP CR 20 30 2E 33 20 20 0D
NE “4.4” 7 SP 4 . 4 SP SP CR 20 34 2E 34 20 20 0D
LY “1.3” 7 SP 1 . 3 SP SP CR 20 31 2E 33 20 20 0D
MO “0.2” 7 SP 0 . 2 SP SP CR 20 30 2E 32 20 20 0D
EO “0.2” 7 SP 0 . 2 SP SP CR 20 30 2E 32 20 20 0D
BA “0.0” 7 SP 0 . 0 SP SP CR 20 30 2E 30 20 20 0D
RBC “5.10” 7 5 . 1 0 SP SP CR 35 2E 31 30 20 20 0D
HGB “14.4” 7 1 4 . 4 SP SP CR 31 34 2E 34 20 20 0D
HCT “42.3” 7 4 2 . 3 SP SP CR 34 32 2E 33 20 20 0D
MCV “86.2” 7 8 6 . 2 SP SP CR 38 36 2E 32 20 20 0D
MCH “28.5” 7 2 8 . 5 SP SP CR 32 38 2E 35 20 20 0D
MCHC “33.1” 7 3 3 . 1 SP SP CR 33 33 2E 31 20 20 0D
RDW “11.5” 7 1 1 . 5 SP SP CR 31 31 2E 35 20 20 0D
PLT “280” 7 SP 2 8 0 SP SP CR 20 32 38 30 20 20 0D
PCT “0.15” 7 0 . 1 5 SP SP CR 30 2E 31 35 20 20 0D
MPV “7.2” 7 SP 7 . 2 SP SP CR 20 37 2E 32 20 20 0D
PDW “18.5” 7 1 8 . 5 SP SP CR 31 38 2E 35 20 20 0D
Reserve data 210 SP × 210 Byte 20 × 210 Byte
Flag data (with flag +, without flag (space))
Leukocytosis “+” 2 + CR 2B 0D
Leukopenia “+” 2 + CR 2B 0D
Neutrophilia “+” 2 + CR 2B 0D
Neutropenia “+” 2 + CR 2B 0D
Lymphocytosis “+” 2 + CR 2B 0D
Lymphopenia “+” 2 + CR 2B 0D
Monocytosis “+” 2 + CR 2B 0D
Eosinophilia “+” 2 + CR 2B 0D
Basophilia “+” 2 + CR 2B 0D
Blasts “+” 2 + CR 2B 0D
Immature “+” 2 + CR 2B 0D
Left Shift “+” 2 + CR 2B 0D
Atypical “+” 2 + CR 2B 0D
WBC hemolysing “+” 2 + CR 2B 0D
Reserve data 20 SP × 20 Byte 20 × 20 Byte
Erythrocytosis “+” 2 + CR 2B 0D
Anemia “+” 2 + CR 2B 0D
Anisocytosis “+” 2 + CR 2B 0D
Microcytosis “+” 2 + CR 2B 0D
Macrocytosis “+” 2 + CR 2B 0D
Hypochromia “+” 2 + CR 2B 0D
MCHC error “+” 2 + CR 2B 0D
Reserve data 10 SP × 10 Byte 20 × 10 Byte
Thrombocytosis “+” 2 + CR 2B 0D
Thrombocytopenia “+” 2 + CR 2B 0D
PLT Clumps “+” 2 + CR 2B 0D
Reserve data 10 SP × 10 Byte 20 × 10 Byte
Reserve data
Reserve data 400 SP × 400 Byte 20 × 400 Byte
<End> Common data block
End of Text 0x03 1 ETX 03

Technical Reference Manual MEK-7222J/K 13

Transferring Item Description

Item No. Item No. of Bytes Description

Hematology analyzer information
1 Type 11 Sets the model of the hematology analyzer “MEK-7222”.
2 Parameter no 6 Sets the number of measurable parameters “22”.
3 Send data bytes 6 Set the total number of transferring data “1024”.
Sets the sampling method.
Measuring 22 or 8 parameters: “VENOUS”
4 Sample type 13 Closed mode with cap pierce unit installed: “CLOSED”
Open mode with cap pierce unit installed: “VENOUS”
Capillary mode: “CAPILLARY”
Sets the measuring parameters.
5 Parameter 13 When measuring 22: CBC + Diff
When measuring 8: CBC
Sets the code for sample type.
6 Sample code 3 When measuring hematology control: “00”
When measuring others: one 2-number code from “01” to “05” is set.
7 Sample type name 17 Sets the sample type.
Sets the sample rack location number. On MEK-7222, there is no
8 Rack location 5
sample rack and “0000” is set.
9 Seq# 11 Sets the sequence number.
10 Reserve data 43 Sets space (0x20)×43.
Measurement data
1 Date 17 Sets date. Year, month and day are separated by CR (0x0D).
2 Time 9 Sets time. Hour, minute and second are separated by CR (0x0D).
3 ID 16 Sets the sample ID number.
4 WBC to PDW 7 each Sets measured data (4 bytes) and flag (2 bytes).
5 Reserve data 210 Sets space (0x20)×210.
Flag data
Leukocytosis to
1 2 each Sets “+” when there is a flag, space (0x20) when there is no flag.
Atypical
2 Reserve data 20 Sets space (0x20)×20.
Erythrocytosis to
3 2 each Sets “+” when there is a flag, space (0x20) when there is no flag.
Abnormal MCHC
4 Reserve data 10 Sets space (0x20)×10.
Thrombocytosis to
5 2 each Sets “+” when there is a flag, space (0x20) when there is no flag.
PLT Clumps
6 Reserve data 10 Sets space (0x20)×10.
Reserved data
1 Reserve data 400 Sets space (0x20)×400.

NOTE
• It is recommended to program the PC to receive data from the hematology analyzer so that the received items
are differentiated by the No. of Bytes for each item and the total no. of bytes and not by CR.
• New sample codes may be added when the software is upgraded.
• In the reserved data, new items by character codes may be set when the software is upgraded in the future.

14 Technical Reference Manual MEK-7222J/K

Abnormal mark
Abnormal mark shows following error.

Item Mark Description ItemMark Description

H Over normal range ! HGB voltage high
All
L Under normal range HGB ? HGB voltage low
? WBC sample error * HGB circuit error
WBC ! Poor hemolyzation MCHC ! RBC sample error
C PLT clumps ? RBC sample error
PLT
RBC ? RBC sample error C PLT clumps

Abbreviation of the alarm and example of the RS-232C data output format
Example: PLT clumps
WBC LEVEL 1 Transfer code: 0x4C, 0x45, 0x56, 0x45, 0x4C, 0x31, 0x0D

Alarm name Code WBC RBC

WBC fluid level 1 A021 LEVEL 1
WBC fluid level 2 A022 LEVEL 2
WBC fluid level 3 A023 LEVEL 3
WBC bubble 1 A024 BBL 1
WBC bubble 2 A025 BBL 2
WBC bubble 3 A026 BBL 3
WBC bubble 4 A027 BBL 4
WBC clogged A029 CLOG
WBC hardware noise A031 NOISE 2
WBC software noise A032 NOISE 1
RBC fluid level 1 A041 LEVEL 1
RBC fluid level 2 A042 LEVEL 2
RBC fluid level 3 A043 LEVEL 3
RBC bubble 1 A044 BBL 1
RBC bubble 2 A045 BBL 2
RBC bubble 3 A046 BBL 3
RBC bubble 4 A047 BBL 4
RBC clogged A049 CLOG
RBC hardware noise A051 NOISE 2
RBC software noise A052 NOISE 1

Technical Reference Manual MEK-7222J/K 15

Explanation of low reliable indicator and example of RS-232C data output
Example: PLT clumps
WBC 7.3C Transfer code: 0x20, 0x37, 0x2E, 0x33, 0x43, 0x20, 0x0D
PLT 280C Transfer code: 0x20, 0x32, 0x38, 0x30, 0x43, 0x20, 0x0D

Parameters
Flag PCT
Flag NE% LY% MO% EO% BA%
Class. WBC RBC HGB HCT MCHC PLT MPV
NE# LY# MO# EO# BA#
PDW
PLT Clumps RBC/PLT C C C
Abnormal MCHC !
Specimen
Poor hemolyzation !

Parameters
Alarm message Code No. NE% LY% MO% EO% BA%
WBC RBC HGB HCT MCHC PLT
NE# LY# MO# EO# BA#
WBC sample error A030 ?
RBC sample error A050 ? ?
HGB voltage low A061 ?
HGB voltage high A062 !
HGB circuit error A063 *

16 Technical Reference Manual MEK-7222J/K

 Socket Pin Assignment

CAUTION
Connect only the specified instrument to the hematology analyzer
and follow the specified procedure. Failure to follow this instruction
may result in electrical shock or injury to the operator, and cause fire
or instrument malfunction.

Serial Port 1
D sub 25 pin (female)

Pin No. Signal Pin No. Signal Pin No. Signal

1 NC 10 NC 19 NC
25 13
2 TXD 11 NC 20 NC
3 RXD 12 NC 21 NC
4 RTS 13 NC 22 NC
5 CTS 14 NC 23 NC
14 6 DSR 15 NC 24 NC
1
7 SG 16 NC 25 NC
8 NC 17 NC
9 NC 18 NC

Bar Code Socket (Serial Port 2)

D sub 9 pin (male)

Pin No. Signal Pin No. Signal Pin No. Signal

1 FG 4 DTR 7 RTS
6 1
2 RXD 5 SG 8 CTS
3 TXD 6 DSR 9 VCC
9 5

Technical Reference Manual MEK-7222J/K 17

Printer Socket
Centronics Interface 36 pin (male)
57GE-40360-751

Pin No. Signal Pin No. Signal Pin No. Signal

1 DSTB 13 SLCT 25 GND
36 18 2 DO 14 NC 26 GND
3 D1 15 NC 27 GND
4 D2 16 GND 28 GND
5 D3 17 GND 29 GND
6 D4 18 P5V 30 GND
19 1
7 D5 19 GND 31 PRIME
8 D6 20 GND 32 FAULT
9 D7 21 GND 33 GND
10 ACK 22 GND 34 NC
11 BUSY 23 GND 35 NC
12 PE 24 GND 36 NC

USB Socket
Pin No. Signal
1 NC
2 USB-D+
3 USB-D-
4 ED

18 Technical Reference Manual MEK-7222J/K