Morra 2018
Morra 2018
Morra 2018
DOI: 10.1002/rmv.1977
REVIEW
Tran Ngoc Xuan Thy2,3 | Nguyen Lam Vuong2,3 | Mostafa Reda Mostafa2,8 |
Sarah Ibrahim Ahmed9 | Sahar Samy Elabd10 | Samreen Fathima11 | Tran Le Huy Vu12 |
1
Faculty of Medicine, Alazhar University, Cairo, Egypt
2
Online Research Club (http://www.onlineresearchclub.org/)
3
University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam
4
Faculty of Medicine, Minia University, Minya, Egypt
5
Department of Cardiology, Shebin El‐Kom Teaching Hospital, Shibin El Kom, Egypt
6
University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
7
Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam
8
Faculty of Medicine, Tanta University, Tanta, Egypt
9
Faculty of Medicine, Cairo University, Giza, Egypt
10
Faculty of Medicine, Benha University, Benha, Egypt
11
Deccan College of Medical Sciences, University of Health and Sciences, Hyderabad, India
12
University of California, Los Angeles, California, USA
13
Department of Medicine, Section of Infectious Diseases, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia
14
Ministry of Health, Riyadh, Saudi Arabia
15
Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Leading Graduate School Program, and Graduate School of Biomedical Sciences, Nagasaki
University, Nagasaki, Japan
16
Evidence Based Medicine Research Group & Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam
17
Department of Clinical Product Development, Institute of Tropical Medicine (NEKKEN), Leading Graduate School Program, and Graduate School of Biomedical
Sciences, Nagasaki University, Nagasaki, Japan
Correspondence
Nguyen Tien Huy, Evidence Based Medicine Summary
Research Group & Faculty of Applied Sciences, Middle East respiratory syndrome (MERS) is a respiratory disease caused by MERS
Ton Duc Thang University, Ho Chi Minh City,
Vietnam. coronavirus. Because of lack of vaccination, various studies investigated the therapeu-
Email: [email protected] tic efficacy of antiviral drugs and supportive remedies. A systematic literature search
Funding information
from 10 databases was conducted and screened for relevant articles. Studies reporting
Japan Initiative for Global Research Network
on Infectious Diseases (J‐GRID); Ministry of information about the treatment of MERS coronavirus infection were extracted and
Education, Culture, Sports, Science, and
analyzed. Despite receiving treatment with ribavirin plus IFN, the case fatality rate
Technology (MEXT) of Japan, Grant/Award
Number: 16H05844, 2016‐2019 was as high as 71% in the IFN‐treatment group and exactly the same in patients
List of abbreviations: CART, the classification and regression tree model; CHF, congestive heart failure; CI, confidential intervals; CRD, chronic renal disease; DM,
diabetes mellitus; ECMO, extracorporeal membrane oxygenation; MD, mean difference; MERS‐CoV, Middle East respiratory syndrome coronavirus; MERS, Middle
East respiratory syndrome; NPV, negative prediction value; PPV, positive prediction value; SARS, severe acute respiratory syndrome; SPSS, Statistical Package for
Social Sciences
Mostafa Ebraheem Morra, Le Van Thanh, and Mohamed Gomaa Kamel equally contributed in the work.
Rev Med Virol. 2018;e1977. wileyonlinelibrary.com/journal/rmv Copyright © 2018 John Wiley & Sons, Ltd. 1 of 9
https://doi.org/10.1002/rmv.1977
2 of 9 MORRA ET AL.
who received supportive treatment only. Having chronic renal disease, diabetes
mellitus and hypertension increased the risk of mortality (P < .05), and chronic renal
disease is the best parameter to predict the mortality. The mean of survival days from
onset of illness to death was 46.6 (95% CI, 30.5‐62.6) for the IFN group compared
with 18.8 (95% CI, 10.3‐27.4) for the supportive‐only group (P = .001). Delay in
starting treatment, older age group, and preexisting comorbidities are associated with
worse outcomes. In conclusion, there is no difference between IFN treatment and sup-
portive treatment for MERS patients in terms of mortality. However, ribavirin and IFN
combination might have efficacious effects with timely administration and monitoring
of adverse events. Large‐scale prospective randomized studies are required to assess
the role of antiviral drugs for the treatment of this high mortality infection.
KEY W ORDS
1 | I N T RO D U CT I O N 2 | METHODS
Middle East respiratory syndrome (MERS) is a viral respiratory disease 2.1 | Search strategy and selection criteria
caused by MERS coronavirus (MERS‐CoV), and it is relatively new to
Our study was performed according to the recommendations of
humans.1 The first report took place in Saudi Arabia in 2012.2 After-
the Preferred Reporting Items for Systematic Reviews and Meta‐
wards, MERS cases were reported across a wide geographic distribu-
Analyses.13 We developed a protocol of methods and registered it in
tion around the globe. However, the vast majority of these cases
the international prospective register of systematic reviews (PROS-
were directly or indirectly linked to traveling or residing in the Middle
2 PERO) (reference, CRD42015024819). In June 2015, we conducted
East. There is no vaccine against MERS‐CoV, and current recommen-
a systematic search of 10 databases including PubMed, Scopus, Web
dations advise people to only adhere to general preventive measures
of Science, Google Scholar, WHO Global Health Library, Virtual Health
such as handwashing, frequently disinfecting touched surfaces, and
2 Library (containing Cochrane, MEDLINE, LILACS, IBECS, and SciELO),
avoiding personal contact with infected populations.
POPLINE, New York Academy of Medicine Grey Literature Report
The clinical picture of the infection ranges from asymptomatic or
(NYAMGLR), SIGLE, and ClinicalTrials.gov. Search results were limited
mild respiratory symptoms to severe acute respiratory disease and
to references published since January 1, 2012, when MERS was first
death.3 Most infected patients present with flu‐like symptoms (cough,
detected. In addition, the human filter was applied in the case of
shortness of breath, fever, and chills) then rapidly progress into severe
PubMed.
illness with pneumonia and acute respiratory distress symptoms, for
After removing duplicates, three trained reviewers were assigned
which many of them require mechanical ventilation (72%).4 Further-
to independently screen the titles and abstracts of all references gen-
more, the MERS can be complicated by acute renal failure that requires
erated from the aforementioned search strategy on the basis of the
hemodialysis and by disseminated intravascular coagulation with
2,4-9 following inclusion and exclusion criteria. Inclusion criteria were (a)
multiorgan failure contributing to the high mortality of the disease.
any study that gives information about the treatment of MERS‐CoV
Currently, there is no reliable remedy for MERS, and all interven-
infection, (b) all types of study designs were included, and (c) no restric-
tions range from supportive to antiviral therapy. Two in vitro studies
tion was made with respect to language, age, and area. Exclusion
suggested a possible efficacious effect of interferon alpha‐2b and riba-
10,11 criteria were (a) data that could not be reliably extracted, (b) data sets
virin in the treatment of MERS infection. Consequently, the inves-
considered as overlapping, (c) studies published before 1/1/2012, (d)
tigators further examined the efficacy of these drugs in an animal
book chapter, thesis, letter, conference paper, poster, or editorial,
study.12 Potential benefits of these antiviral treatments in both
and (e) animal or in vitro studies. Three reviewers compared their
in vitro and animal studies persuaded clinicians to question the feasibil-
screening results and discussed the differences. A consensus was
ity and applicability of such an approach in humans. Therefore, we
reached through discussion.
aimed to recapitulate the evidence from all human published data
about MERS clinical management in a systematic review and meta‐
analysis, to summarize the efficacy and safety of current applied ther-
apeutics and define risk factors associated with outcomes. As a result,
2.2 | Data extraction
we may provide a better approach to more compatible management of Extracted data included publication year, year of research, country and
fatal consequences of MERS infections and the risk imposed by recent city of the patients, year of subject recruitment, study design, partici-
outbreaks in densely populated areas. pant enrollment, data collection method, baseline characteristics
MORRA ET AL. 3 of 9
Total Number of
Study Name Study Design Country Age Male Types of Treatment Cases Deaths
TABLE 2 Comparison between IFN and comparator groups (no The quality of 4 observational studies22,27,29,32 was assessed using
antiviral/IFN) the 9 metrics tool15 (Table S2). Three of them had a score23,27,29 of 5 of
Treatment with Comparator 9 as they described study design, characteristics of the patient popula-
Factor IFN (n = 68) Group (n = 48) P Value tion, inclusion criteria, method quality, and MERS diagnosis. However,
Age (mean) 62.2 57.3 none of the 3 studies described data collection method, assignment
Gender (males) 48 (71) 40 (83) .114 method of patients, exclusion criteria, and interpretation.
Mortality 48 (71) 34 (71) 1
Diabetes 38 (56) 26 (54) .862
Hypertension 26 (38) 9 (19) .024
3.4 | Treatment with antiviral drugs
Chronic renal impairment 22 (32) 11 (23) 0.27 Treatment with antiviral drugs, other than oseltamivir, was reported in
a
Dialysis dependent 10 (15) 1 (2) .025 9 studies. IFNs (IFN alpha‐2a, alpha‐2b, or beta‐1a) in combination
Congestive heart failure 17 (25) 10 (21) .603 with ribavirin were the common remedies used in all 9 studies.
Other comorbidities 23 (34) 3 (6) .0005a IFN alpha‐2a was used in 4 studies (n = 35 patients),21,27-29 all of
Number of comorbidities 136 60 which used a dose of 180 μg/wk for treatment. IFN alpha‐2b was used
Invasive ventilation 52 (76) 43 (90) .071 in 5 studies (n = 22)22,25,26,30,32 with a dose ranging between 100 and
Renal replacement 17 (25) 22 (46) .019 180 μg/wk. IFN alpha‐2b dose was not reported in Al‐Hameed et al.32
Corticosteroids 28 (41) 28 (58) .068 IFN beta‐1a was used in 2 studies (n = 12), both of which used a dose
Oseltamivir 31 (46) 31 (65) .043 of 44 μg/wk for treatment.28,29
Vasopressors 25 (37) 32 (67) .002 Ribavirin administration was started with a loading dose followed
Prone position 4 (6) 6 (12.5) .315a by subsequent doses in all 9 studies. The loading dose was 2000 mg
ECMO 3 (3) 5 (10.4) .272a for all studies while 400 mg in one study of AlGhamdi et al.21 The sub-
with ritonavir‐boosted atazanavir (atazanavir 300 mg plus ritonavir 116, 81.9%) required the use of invasive mechanical ventilation:
100 mg) orally once daily. Similarly, triple therapy with IFN alpha‐2a, 76.4% of patients in IFNs treatment group (52 of 68) compared with
ribavirin, and lopinavir/ritonavir (400/100 mg twice daily) was used 90% of patients in supportive treatment–only group (43 of 48).
to treat the Greek patient in Spanakis et al.30
It is worth mentioning that there was a significant lag period 3.6 | Therapeutic outcomes
between presentation and initiation of the antiviral treatment. The Regarding mortality, studies with more than 2 cases were included in
period from the admission of patients to the initiation of antiviral (IFNs the meta‐analysis. The pooled proportion of mortality was 0.714
and ribavirin) was reported in 13 patients and had a mean of 12 days (0.618‐0.795) from 8 studies including 106 MERS patients (Figure 2).
and ranged between 1 day in Khalid et al26 and 21 days in Al‐Tawfiq In 68 patients received IFN treatment, the mortality rate was high
et al (Table S3).22 (71%) in spite of receiving treatment with ribavirin plus IFN (alpha‐
2a, alpha‐2b, or beta‐1a). Likewise, the mortality rate was high in
3.5 | Supportive treatment patients who received supportive treatment only (71%, n = 48). There
Supportive treatment alone was used in 7 studies (n = 24 patients), was no statistical significant mortality difference when comparing mor-
whereas supportive treatment along with antiviral medications was tality of both groups (P = 1) (Table 2). The same insignificant mortality
used in another 8 studies (n = 48) and one study compared support- difference was shown when comparing 3 types of IFN treatments: IFN
ive/antiviral medications against supportive treatment alone (n = 20 alpha‐2a, IFN alpha‐2b, and IFN beta‐1a (P = .65) (Table 3).
and 24, respectively). Different modalities of treatments were used The duration from hospitalization to death was reported in 78 of
to support the patients, including invasive ventilation, extracorporeal 82 deceased patients. All 78 reported patients died within 3 months
membrane oxygenation, corticosteroids, renal replacement therapy, of hospitalization. Moreover, 59% of the 82 patients died within the
vasopressors, oseltamivir, and prone positioning. Most patients (95 of first 2 weeks of admission, and 79% died within 1 month of admission.
FIGURE 2 Forest plot meta‐analysis of 8 studies regarding the mortality rate of patients with MERS. CI, Confidence interval; P, probability value
Factor IFN Alpha‐2a (n = 34) IFN Alpha‐2b (n = 22) IFN Beta‐1a (n = 11) P Value
Age (mean) 65.8 57.1 67
Gender (males) 27 (79) 16 (73) 4 (36) .024
Mortality 26 (76) 15 (68) 7 (64) .65
Diabetes 24 (71) 9 (41) 5 (45) .065
Hypertension 11 (32) 8 (36) 7 (64) .173
Chronic renal impairment 11 (32) 8 (36) 3 (27) .869
Dialysis dependent 4 (12) 4 (18) 2 (18) .723a
Congestive heart failure 10 (29) 5 (23) 2 (18) .715
Other comorbidities 13 (3) 10 (9) 0 .0027
Invasive ventilation 30 (85) 16 (73) 6 (55) .052
Renal replacement therapy 12 (35) 5 (23) … .317
Corticosteroids 11 (32) 16 (73) … .003
Oseltamivir 18 (53) 13 (59) … .655
Vasopressors 14 (41) 11 (50) … .517
Prone position 4 (12) 0 … .146a
ECMO 2 (6) 1 (5) … 1 a
In a case series of 6 patients from Saudi Arabia by Khalid et al,26 3 only 2 patients of 12 survived patients (P < .001). For continuous var-
patients had comorbid conditions and needed mechanical ventilation iables, there was a significant difference between death and survival in
before dying, while the other 3 patients who did not require mechan- age, where the dead patients were older, and time from onset of illness
ical ventilation survived. A case report from Greece described one to initiation of antiviral treatment, being longer in the deceased
patient with MERS‐CoV infection who died despite receiving IFN patients (P < .05) (Table 4).
alpha‐2a, ribavirin, and lopinavir. The patient had a multiorgan failure
and was later diagnosed with colon cancer. MERS‐CoV was not detect- 3.8 | CART model
able in his respiratory tract until several days before death.30
The modeling tool, CART, identified CRD as the best parameter to pre-
Regarding the adverse effects of interferon and ribavirin combina-
dict mortality (Figure 3A). The performance of the decision tree tool
tion therapy, 2 patients had pancreatic enzyme elevation, and one had
that classified mortality outcome was at an accuracy of 72.4%, sensi-
significant hemolysis when being treated with IFN alpha‐2a plus ribavi-
tivity of 52.9%, specificity of 100%, PPV of 100%, and NPV of 60%.
rin.22 In addition, the reduction in hemoglobin level was 4.32 g/L in 20
In addition, treatment with inotropes was exhibited as the best param-
MERS patients treated with IFN alpha‐2b plus ribavirin, whereas
eter to predict renal replacement therapy outcome (Figure 3B). The
Omrani et al27 reported only 2.14 g/L of hemoglobin reduction in 24
performance of the decision tree tool that classified renal replacement
patients treated with supportive‐only treatment (P = .002).
therapy outcome was at an accuracy of 86.2%, sensitivity of 66.67%,
specificity of 95%, PPV of 85.7%, and NPV of 86.4%. No significant
3.7 | Risk factors associated with outcomes
results were detected regarding the invasive ventilation outcome.
There was a significant difference between death and survival in
patients with chronic renal disease (CRD). Nine CRD patients died of
17 deceased cases, and no patient had CRD in the survival group. In
3.9 | Survival rate over time
addition, diabetes mellitus (DM) and hypertension showed similar sig- Survival time from admission to death was compared for IFN‐treated
nificant variation, suggesting that having CRD, hypertension, and/or (n = 30) and for non‐IFN (supportive care only, n = 14) patients. All
DM increased the risk of mortality (P < .05). There was no significant 44 cases died within 80 days after hospital admission (Figure 4A). Only
difference between death and survival regarding gender, ribavirin, cor- one female case treated with IFN was eliminated from analysis
ticosteroid, oseltamivir, IFN beta‐1a, IFN alpha‐2b, IFN alpha‐2a, con- because the patient remained intubated when the original study
gestive heart failure, and other comorbidities (P > .05). All 17 deceased ended. However, she had met death criteria; thus, she was included
patients required mechanical ventilation before dying compared with with deceased patients in the final outcome analysis. The mean
Age, mean (SD) 56.4 (21.6) years 39.8 (13.3) years 16.6 (3.3‐30.0) … .014
Time from admission to antiviral 15.1 (4.4) days 1.7 (0.6) days 13.4 (10.2‐16.6) … .003
treatment start, mean (SD)
Gender (male), % 15 (88) 10 (83) 1.5 (0.18‐12.46) 1
DM 7 0 … .023
HTN 6 0 … .028
CRD 9 0 … .003
DD 4 0 … .12
CHF 2 0 … .498
Other comorbidities 10 3 4.29 (0.84‐21.76) .13
IFN alpha‐2a 1 1 0.69 (0.04‐12.20) 1
IFN alpha‐2b 9 5 1.58 (0.35‐7.00) .71
IFN beta‐1a 1 0 … .414
Ribavirin 10 6 1.43 (0.32‐6.32) .716
Ventilation 17 2 … <.001
Corticosteroid 8 6 0.89 (0.20‐3.90) 1
Oseltamavir 10 4 2.86 (0.61‐13.34) .264
Inotropes 6 1 6.00 (0.62‐58.43) .187
Renal therapy 8 1 9.80 (1.02‐93.50) .043
Abbreviations: CHF, congestive heart failure; CI, confidential interval; CRD, chronic renal diseases; DD, dialysis dependent; DM, diabetes mellitus; HTN,
hypertension; MD, mean difference; SD, standard deviation.
Other comorbidities: colon adenocarcinoma, HIV, renal transplant, asthma, sleep apnea, coronary artery disease, atrial fibrilation, ischemic heart disease,
right bundle branch block, cardiomyopathy, MI, dyslipidemia, histamine induced angioedema, multiple myeloma, and obesity.
Significant values are in bold.
a
Numbers are the frequency and percent (%) otherwise stated.
MORRA ET AL. 7 of 9
FIGURE 3 The classification and regression tree model of 29 cases with individual data. A, Chronic renal disease was the best prediction variable
for mortality rate. B, Inotropes was best prediction variable for renal replacement therapy
FIGURE 4 Kaplan‐Meier survival curves showing death days over time in IFN‐treated patients and supportive‐only groups. A, Death days from
hospital admission to death for 44 cases (P = .977) and, B, death days from onset of illness to death for 13 cases (P = .001)
survival days was 21.3 days (95% CI, 14.1‐28.5) for IFN group and Further specific comparison of death versus nondeath cases in
21.4 days (95% CI, 12.4‐30.4) for the supportive‐only group, both IFN and supportive treatments was conducted to get the mean
P = .977. Overall, mean of survival days for both groups was 21.3 days from onset to admission. The mean of days for 10 IFN‐treated
(95% CI, 15.7‐26.9). dead patients and 5 IFN‐treated survivals was 7.79 and 4.40, respec-
Only 7 cases in the IFN group and 6 cases in the supportive‐only tively, with MD (95% CI) = 3.39 (−3.48 to 10.26), P = .3. Moreover,
group reported information about time from onset of symptoms to the mean of days for 13 supportively treated dead patients and 10
death. A second Kaplan‐Meier survival analysis was performed and supportively treated survivals was 2.69 and 2.1, respectively, with
showed that all of them died within 89 days from onset of illness. The MD (95% CI) = .59 (−1.35 to 2.53), P = .53. The mean of days from
mean of survival days was 46.6 (95% CI, 30.5‐62.6) for the IFN group onset of illness to admission for pooled total cases of death and sur-
compared with 18.8 (95% CI, 10.3‐27.4) for the supportive‐only group. vived was 4.07 and 2.27, respectively, with MD (95% CI) = 1.8
The difference between the 2 groups was statistically significant (−0.81 to 4.41), P = .17.
(P = .001) (Figure 4B). The longer survival period from onset to death
was simply attributed to the duration between onset and admission.
The mean time from onset of illness to the admission of 23 4 | DISCUSSION
patients in IFN group was 6.52 days compared with the same number
of patients in the supportive‐only group who had a mean of 2.43 days. Our systematic review highlights the significance of age and period
The mean difference (MD) of time between 2 groups was also calcu- between the illness onset and start of antiviral therapy in MERS cases'
lated: MD (95% CI) = 4.09 (2.71‐5.47), P < .05. prognostic assessment. Our results revealed that younger age and
8 of 9 MORRA ET AL.
more rapid initiation of antiviral therapy are associated with higher 5 | CO NC LUSIO N
chances of survival. We also found that patients who require ventila-
tion are more likely to die compared with patients who do not require There is no evidence of any difference between IFN treatment and
it. Furthermore, there is no difference between IFN treatment and sup- supportive treatment for MERS patients in terms of mortality. The
portive treatment in terms of mortality rate and no significant effect on ribavirin and IFN combination might have promising effects where
the duration of survival in infected patients' different groups. therapy can be started promptly and adverse effects monitored care-
Several factors can affect the clinical outcomes of MERS therapy. fully; a randomized controlled trial is required to assess this possibility.
In our data, there was a significantly higher number of patients with Concerning prognostic factors, delayed treatment, older age, and
hypertension and dialysis dependence in the IFN group compared with accompanying comorbidities such as hypertension, DM, chronic kidney
the supportive‐only group. The survival time of patients receiving disease, and dialysis dependence are associated with worse outcomes.
antiviral therapy and the efficacy of the drugs were likely reduced Because of high fatality, the seriousness of this newly emerging dis-
because of hypertension and dialysis dependence. Nevertheless, upon ease, and a limited number of available cases, we believe there is an
investigating the outcomes of 13 patients under treatment for MERS, urgent need for large‐scale clinical trials on the efficacy of antiviral
we found that, despite the higher number of comorbidities in the IFN treatment of MERS‐CoV infections.
group compared with the supportive‐only group, patients on IFN
survived considerably longer from onset of symptoms than those on CONFLIC T OF IN TE RE ST
supportive therapy. This result highlights the potential efficacy of The authors declare no competing interests.
antiviral treatment in MERS‐CoV infection. However, the number of
cases is small to draw any conclusions, and further studies are FUNDING
definitely required.
This work was supported in part by a “Grant‐in‐Aid for Scientific
In terms of different supportive remedies, corticosteroids did not
Research (B)” (16H05844, 2016‐2019, for Nguyen Tien Huy) from
show any promising effects on the survival of infected patients, which
the Ministry of Education, Culture, Sports, Science, and Technology
is also consistent with previous studies.8,23,27,33 Patients with signs of
(MEXT) of Japan and by the Japan Initiative for Global Research Net-
severe respiratory distress, shock, or hypoxemia should be given oxy-
work on Infectious Diseases (J‐GRID) for Kenji Hirayama. The funders
gen therapy immediately, and those who cannot maintain a SpO2 ≥ 90%
had no role in the study design, data collection and analysis, decision to
with oxygen therapy should be considered for intubation and mechan-
publish, or preparation of the manuscript.
ical ventilation. However, noninvasive ventilation should be avoided
because of the high risk of spreading the infection.34 Patients who
AUTHOR CONTRIBUTIONS
have an indication for ventilation were more likely to have an accom-
panying serious condition compared with other patients,34 which could N.T.H., A.A.G., M.E.M., and K.H. participated in the design of the study.
explain the higher risk of mortality among those patients as shown in L.V.T., L.M.D., M.G.K., N.L.V., and A.M.A.A. performed in the analysis
our results. and interpreted it. All authors contribute the screening and data
Because of high case fatality rate and faster progression to respi- extraction, wrote the manuscript, read, and approved the final
ratory failure, it will be pressing to distinguish MERS from severe acute manuscript.
clinical features.35 Our results obtained with CART modeling suggest Ahmed Abdelmotaleb Ghazy http://orcid.org/0000-0002-9145-
that chronic respiratory disease can be the best predictor of death in 7115
patients with MERS. Therefore, it is of utmost necessity to thoroughly Nguyen Tien Huy http://orcid.org/0000-0002-9543-9440
assess and continuously monitor the respiratory functions of patients
with suspected MERS‐CoV infection. Also, health care agencies in high RE FE RE NC ES
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precautious measures that are required to face any future outbreaks.
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