Abbas 2009 Caracterizacion Harina Banano
Abbas 2009 Caracterizacion Harina Banano
Abbas 2009 Caracterizacion Harina Banano
Abstract: Physical properties of ripe banana flour were studied in Cavendish and Dream banana, in order to
distinguish the two varieties. Flour was analyzed for pH, total soluble solids (TSS), water holding capacity
(WHC) and oil holding capacity (OHC) at 40, 60 and 80 °C, color values L*, a* and b*, back extrusion force
and viscosity. Physical properties data were analyzed by cluster analysis (CA) and discriminant analysis (DA).
CA showed that the two types of flour were different in terms of selected physical properties. DA indicated that
WHC at 60 °C was the main contributor in discriminating the two types of flour.
Keyword: Physical properties, ripe banana flour, cluster analysis, discriminant analysis.
*Corresponding author.
Email: [email protected]
Tel.: 6 04 6533888 (ext 2222). Fax.: 6 04 6573678 © All Right Reserved
184 Abbas F. M. A., Saifullah, R. and Azhar, M. E.
Material and Methods supernatant was decanted, and the tubes were allowed
to drain for 10 min at a 45o angle. The residue was
Preparation of ripe banana flour weighed and WHC and OHC calculated as g water
Two banana varieties, namely Cavendish (Musa or oil per g dry sample, respectively (Rodrı´guez-
paradisiaca L, cv cavendshii) and Dream (Musa Ambriz et al., 2008).
acuminata colla. AAA, cv ‘Berangan’) banana, were
purchased from eleven different markets around Color
Penang Island, Malaysia. A total of 70-80 ripe banana The instrumental measurement of banana flour
(stage 5 of ripeness – more yellow than green) of color was carried out with a Colorimeter Minolta
each variety were obtained from each market (A total CM-3500d (Minolta, Spectrophotometer, USA) and
of 1648 bananas for all samples). The ripeness stage the results were expressed in accordance with the
5 was selected since this stage corresponds to various CIELAB system with reference to illuminant D65
uses in industrial transformation and traditional and a visual angle of 10o. The measurements were
culinary preparations (Emaga et al, 2008). The fruits performed through a 6.4-mm-diameter diaphragm
were peeled and cut into transverse slices of about 2 with an optical glass, placing the flour directly on the
mm thickness. Slices were then dipped in 0.5 % (w/v) glass. The parameters determined were L* (L* = 0
sodium metabisulphite solution for 5 min, drained [black] and L* = 100 [white]), a* (−a* = greenness
and dried in oven (AFOS Mini Kiln) at 60oC for 18 and +a* = redness), b* (−b* = blueness and +b* =
hrs. The dried samples were ground in a Retsch Mill yellowness).
Laboratory (Retsch AS200) to pass through 60 mesh
screen to obtain banana flour. The yield of flour was Back extrusion force of slurry
calculated by dividing the amount of flour produced A TA-XTplus Texture Analyzer (Stable Micro
by the amount of fresh banana used, and the results Systems, Godalming, UK) was used to evaluate the
were converted to g/Kg (g of flour/Kg of banana). texture of the banana flour slurry (10% w/v). Back
The flour was stored in airtight plastic packs in cold ward extrusion tests were conducted with the disc
storage (15±2°C) for further analyses. diameter 45 mm, setting the probe travel distance at
30 mm. Both tests were performed with a test speed
pH, total soluble solid and viscosity of 5 mm/s, a trigger force of 5 × g, and force in
The pH of the flour was measured using a Inlab compression mode. Force-time curves were recorded
421 Electrode attached with Delta 320 pH meter at a crosshead speed of 5 mm/s and recording speed
(Mettler-Toledo, Switzerland). Flour dispersion was 5 mm/s to enable evaluation of back extrusion
(8% (w/v)) was stirred for 5 min, allowed to stand force (BEF) of the slurry. BEF of slurry may indicate
for 30 min, filtered and the pH of filtrate measured gelation property potentials of the flour.
(Suntharalingam and Ravindran, 1993). Total soluble
solids (TSS) in the same flour slurries were measured Multivariate statistical methods
using an Atago refractometer (Atago PAL-1, Co. Ltd.,
Tokyo, Japan) (Salvador and Fiszman, 2007). Cluster analysis
Viscosity was determined as described by Cluster analysis (CA) is a multivariate technique,
Fagbemi (1999). Flour was dispersed in water at 8% whose primary purpose is to classify the objects of
(w/v) concentration using a magnetic stirrer (1000 the system into categories or clusters based on their
rpm) and heated from 30 to 95oC in a waterbath similarities, and the objective is to find an optimal
and kept at this temperature for 20 min. The slurry grouping for which the observations or objects
obtained was stirred constantly and cooled at room within each clusters are similar, but the clusters are
temperature. The viscosity was measured using a dissimilar to each other. Hierarchical clustering is
Brookfield Viscometer, model DV-E (Brookfield the most common approach in which clusters are
engineering laboratories, Inc, Middleboro, MA, formed sequentially. The most similar objects are
USA) using spindle 3, at 50 rpm. first grouped, and these initial groups are merged
according to their similarities. Eventually as the
Water-holding capacity (WHC) and oil-holding similarity decreases all subgroups are fused into a
capacity (OHC) single cluster. CA was applied to physical properties
Twenty-five millilitres of distilled water or of banana flour using a linkage method. In the linkage
commercial olive oil were added to 1 g of dry method, the distances or similarities between two
sample, stirred and incubated at 40, 60 or 80oC for 1 clusters A and B is defined as the minimum distance
h. Tubes were centrifuged at 3000 x g for 20 min, the between a point in A and a point in B:
Table 1. Mean and standard deviations (n =11) of selected physical parameters for the two types of banana flour
latter. In principle, this could cause less sweetness yielded a higher WHC (Rodrı´guez-Ambriz et al.,
to be perceived in Cavendish banana flour which 2008). Starch however is not the principal component
in turn could influence consumer acceptance. This of ripe banana (Zhang et al., 2005), leaving dietary
suggestion however, needs further testing since the fibers and protein as the main contributing factors
difference is marginal. TSS indicates soluble solid that influence WHC of ripe banana flour. The highest
content of banana flour, and high TSS has been WHC was attained at 80°C in all banana flour was
associated with high sucrose content in banana pulp also attributed to the effects of the solution properties
(Bugaud et al., 2006). It has been reported that the of dietary fiber (Juarez-Garcia et al., 2006) and to a
average starch content drops from 70 to 80% in the less extent the gelatinization of starch in the flour that
pre-climacteric period to less than 1% at the end of absorbs water into starch granules with concomitant
the climacteric period, while sugars, mainly sucrose, swelling (Rodrı´guez-Ambriz et al., 2008). Good
accumulate to more than 10% of the fresh weight of water holding property implies the potentials of
the fruit (Zhang et al., 2005). As ripe banana was banana flour to be used as a thickener in liquid and
used for flour preparation the pH is expected to be semiliquid foods.
mildly acidic with low acid content, but with a high Another functional property of banana flour is oil
sugar content. holding capacity (OHC). Mean OHC ranged between
Mean WHC increased with temperature, and 0.8 to 1.0 g oil/g dry samples at the three temperatures
ranged between 0.5 – 2.7 g/g dry sample. These assessed. These values are slightly lower than that
values are lower than those reported in mango dietary reported in fiber-rich banana powder that could hold
fiber (12 and 15 g water/g dry sample) and mango peel 2.2 g oil/g dry sample (Rodrı´guez-Ambriz et al.,
dietary fiber (11 g/g) (Larrauri et al., 1996), but were 2008), but are similar to that of mango dietary fiber
similar to those of fiber-rich unripe banana flour (2.5 with OHC in the range 1.0 – 1.5 g oil/g ((Larrauri et
g/g) (Rodrı´guez-Ambriz et al., 2008). The average al., 1996). Other products tested for OHC include
WHC40 and WHC60 were higher in the Cavendish mango peel dietary fiber (~ 4 g oil/g), (Larrauri et al.,
banana flour as compared to that of Dream banana. 1996) and citrus peel fiber (2.35 – 5.09 g oil/g) (Chau
WHC could be related to the physical state of starch and Huang, 2003). OHC relates to the hydrophilic
(Waliszewski et al., 2003), dietary fiber and protein character of starches present in the flour (Rodrı´guez-
in the flour. It was the release of amylose which has Ambriz et al., 2008) that could be present in some
the capacity to effectively bind water molecules that quantity in Cavendish as well as in Dream banana
Figure 1. Dendrogram showing clustering of banana flour samples based on physical properties
Table 4. Classification results for discriminant analysisa of the two types of flour
the variation between the banana flour. The relative the L* and b* values and WHC60. The positive
contribution of the parameters can be ranked as contribution was mainly attributed to positive
follows; coefficient of the parameters, while the negative
contribution was mainly attributed to the negative
L* > b* > WHC60 > viscosity > pH > WHC40 > coefficient of the parameters in equation 2.
OHC40 > OHC80 > OHC60 > WHC80, BEF > a*
> TSS.
Conclusion
Based on this ranking it may be concluded that
L* and b* values and WHC60 are the most important The present study revealed some differences in
physical properties that discriminate ripe Cavendish physical properties of ripe Cavendish and Dream
and Dream banana flour. However since color banana flour. Statistical analysis indicated that water
assessment was problematic due to the presence holding capacity at 60°C were the main contributors
of dark spots and color changes occurred during in discriminating the two types of flours. Future work
drying of banana slices, it would be appropriate to should involve comparison of physical properties
dismiss color parameter as discriminating methods of banana flour prepared from various stages of
to differentiate the flour. TSS is the least reliable ripeness and varieties. This should be supported by
physical parameter to discriminate the flour since the chemical analysis of dietary fibers, starch and sugar
contents of soluble solid in the flours were almost composition in order to explain the differences in
similar (Table 1). physical properties of the flour. Incorporation studies
The classification matrix (Table 4) showed that of ripe banana flour into strategic food products could
100% of the cases were correctly classified to their also be attempted.
respective groups. The results of classification also
showed that significant differences existed between
these two types of flour (Table 4), affording 100% Acknowledgement
correct assignation, which are expressed by in term of
one discriminant function. This result is in agreement The authors gratefully acknowledge the financial
with CA which indicated that the two types of flour assistance from Universiti Sains Malaysia and the
were different based on their physical properties. research facilities by Dean of the School of Industrial
Technology, USM, Penang. USM short term grant
Source identification (304/ PTEKIND/638098) is acknowledged.
Relationship between the scores of
discriminant function and the samples from
various samples (Figure2) corresponded to References
the scores of discriminant function for various
samples. The samples nos. 1-11 corresponded to Alkarkhi, A.F.M., Ramli, S. and Easa, A.M. 2009.
banana samples of each variety. It could be seen Differentiation of ripe banana flour using mineral
composition and logistic regression model.
from Figure2 that all Cavendish samples showed International Food Research Journal, in press.
positive contribution to discriminant function,
whereas Dream banana samples showed negative Alvin, C. R. 2002. Methods of Multivariate Analysis. John
contribution. This difference was mainly due to Wiley and Sons.INC. USA.
Aparicio-Saguilan, A., Sayago-Ayerdi, S.G. , Vargas- Richard A. J. and Dean W. W. 2002. Applied Multivariate
Torres, A., Tovar, J., Ascencio-Otero, T. E. and Bello- statistical analysis. Prentice-Hall, London.
Perez, L. A. 2007. Slowly digestible cookies prepared
from resistant starch-rich lintnerized banana starch. Rodrı´guez-Ambriz, S.L, Islas-Herna´ndez, J.J, Agama-
Journal of Food Composition and Analysis 20: 175- Acevedo, E., Tovar, J. and Bello-Pe´rez, L.A. 2008.
181. Characterization of a fiber-rich powder prepared by
liquefaction of unripe banana flour. Food Chemistry
Bugaud, C., Chillet, M., Beaute, M.P. and Dubois, C. 107: 1515–1521
2006. Physicochemical analysis of mountain bananas
from the French West Indies. Scientia Horticulturae Sothornvit, S. and Pitak, N. 2007. Oxygen permeability
108: 167-172. and mechanical properties of banana films. Food
Research International 40: 365-370.
Chau, C. F. and Huang, Y.L. 2003. Comparison of chemical
composition and physical properties of different Salvador, A. and Fiszman, S.M. 2007. Changes in colour
fiber prepared from the peel of Citrus sinensis L. cv. and texture and their relationship with eating quality
Liucheng. Journal of Agricultural and Food Chemistry during storage of two different dessert bananas.
51: 2615-2618. Postharvest Biology and Technology 43: 319–325
Emaga, T.H., Robert, C., Ronkart, S.N., Wathelet, B. and Suárez, M. H., Rodrĺguez, E. M. R. and Romero, C. D.
Paquot, M. 2008. Dietary fiber components and pectin 2007. Mineral and trace element concentrations in
chemical features of peels during ripening in banana cultivars of tomatoes. Food Chemistry 104: 489-499.
and plaintain varieties. Bioresource Technology 99:
4346-43454. Suntharalingam, S. and Ravindran, G. 1993. Physical and
biochemical properties of green banana flour. Plant
Fagbemi, T.N. 1999) Effect of blanching and ripening on Foods for Human Nutrition 43: 19-27.
functional properties of plantain (Musa aab) flour.
Plant Foods for Human Nutrition 54: 261–269. Vilas-Boas, E. V. de B. and Kader, A.A. 2006. Effect of
atmospheric modification, 1-MCP and chemicals on
Juarez-Garcia, E., Agama-Acevedo, E., Sayago-Ayerdi, quality of fresh-cut banana. Postharvest Biology and
S.G., Rodriguez-Ambriz, S.L. and Bello-Perez, L.A. Technology 39: 155-162.
2006. Composition, digestibility and application in
breadmaking of banana flour. Plant Foods for Human Waliszewski, K.N., Aparicio, M.A., Bello, L.A. and
Nutrition 61: 131-137. Monroy, J.A. 2003. Changes of banana starch by
chemical and physical modification. Carbohydrate
Larrauri, J.A., Ruperez, P., Borroto, B. and Saura-Calixto, F. Polymers 52: 237-242.
1996. Mango peels as a new tropical fiber: Preparation
and characterization. Lebensmittel-Wissenschaft und Yomeni, M.O., Njoukam, J. and Tchango, J. 2004.
Technologie 29: 729-733. Influence of the stage of ripeness of plaintains and some
cooking bananas on the sensory and physicochemical
Markus, P. F., Elena, R. R., Jacinto, D. M. and Carlos, D. R. characteristics of processed products. Journal of the
2002. Statistical differentiation of bananas according Science of Food and Agriculture 84 (9): 1069-1077.
to their mineral composition. Journal of Agricultural
and Food Chemistry 50: 6130-6135. Zhang, P., Whistler, R. L., BeMiller, J. N. and Hamaker,
B. R. 2005. Banana starch: production, physical
Ricardo, C. R., Pablo, S. H., Elena, M. R. R., Jacinto, D. properties and digestibility – a review. Carbohydrate
M. and Carlos D. R. 2003. Mineral concentration in Polymers 59: 443-458.
cultivars of potatoes. Food Chemistry 83: 247-253.