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    CHE Lab Report 02

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    Ceylin Baykoç
    This lab report describes an experiment using UV-visible spectroscopy to determine the concentration of an unknown crystal violet solution. Students prepared serial dilutions of a 1M crystal violet stock solution and measured their absorbance values. A calibration curve was generated from the absorbance measurements of the known concentrations. This was used to determine the concentration of the unknown solution, which was found to be 0.616754M.

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    CHE Lab Report 02

    Uploaded by

    Ceylin Baykoç
    15 views4 pages
    This lab report describes an experiment using UV-visible spectroscopy to determine the concentration of an unknown crystal violet solution. Students prepared serial dilutions of a 1M crystal violet stock solution and measured their absorbance values. A calibration curve was generated from the absorbance measurements of the known concentrations. This was used to determine the concentration of the unknown solution, which was found to be 0.616754M.

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    CHE lab report 02

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    This lab report describes an experiment using UV-visible spectroscopy to determine the concentration of an unknown crystal violet solution. Students prepared serial dilutions of a 1M crystal violet stock solution and measured their absorbance values. A calibration curve was generated from the absorbance measurements of the known concentrations. This was used to determine the concentration of the unknown solution, which was found to be 0.616754M.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    15 views4 pages

    CHE Lab Report 02

    Uploaded by

    Ceylin Baykoç
    This lab report describes an experiment using UV-visible spectroscopy to determine the concentration of an unknown crystal violet solution. Students prepared serial dilutions of a 1M crystal violet stock solution and measured their absorbance values. A calibration curve was generated from the absorbance measurements of the known concentrations. This was used to determine the concentration of the unknown solution, which was found to be 0.616754M.

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    ACIBADEM MEHMET ALI AYDINLAR

    UNIVERSITY

    MOLECULAR BIOLOGY AND GENETICS


    DEPARTMENT
    2022-2023 FALL

    MBG 111
    GENERAL CHEMISTRY LABORATORY
    REPORT 02
    UV VISIBLE SPECTROSCOPY

    Submitted to: Asst. Prof. Tuğba Arzu Özal İldeniz


    Deadline: 6 December 2022

    Group Number: 05
    Group Members
    Ceylin Baykoç 221403022
    Esra Akel 210101038
    Nuri Sarı 210101006
    ABSTRACT

    1. INTRODUCTION

    2. PRE-LAB QUESTIONS

    1. What affects the absorbance of a solution?


    The amount of light of a certain wavelength that inhibits a substance from going
    through is measured as absorbance. There are two main factors that affect the absorbance:
     Concentration of a substance
     Path length

    2. What are the UV and Visible wavelength ranges?

    3. MATERIALS

     Micropipette
     Micropipette tips
     Test tubes
     Crystal violet (1 M)
     Test tubes
     Spectrophotometer
     Cuvette
     Distilled water (dH 2 O)

    4. METHODS

    1- 1 M of crystal violet stock solution was given in test tube by the laboratory assistants.
    2- 5 different test tubes were prepared by diluting the concentration of solution taken in
    each test tube by 1/2.
    3- All test tubes were prepared by diluting the concentration and filled into
    spectrophotometer cuvettes and placed into spectrophotometer compartment.
    4- The solution of unknown concentration was added to the cuvette and put into the
    spectrophotometer machine, and the absorbance value and molarity was measured.
    5- Absorbance values are measured from cuvettes placed in the spectrophotometer.

    5. CALCULATIONS

    Stock tube => 1 M


    First test tube => 0,5 M
    Second test tube => 0,25 M
    Third test tube => 0,125 M
    Fourth test tube => 0,0625 M
    Fifth test tube => 0,03125
    Unknown test tube => 0,616754 M
    6. RESULT AND DATA

    3 mL 3 mL 3 mL 3 mL 3 mL

    1 M Crystal Violet 3 mL dH2O 3 mL dH2O 3 mL dH2O 3 mL dH2O 3 mL dH2O


    stock solution

    1:1 1:2 1:4 1:8 1:16 1:32

    Figure 1.1:

    Table 1.1:

    2.5

    2
    f(x) = 2.1965218104138 x − 0.0217308467017309
    R² = 0.999272797802706

    1.5

    0.5

    0
    0 0.2 0.4 0.6 0.8 1 1.2

    7. DISCUSSION

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