Post Lab Answers

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POST-LAB ANSWERS

1. What kind of enzymes is in the pineapple that works on the gelatin? 


 Fresh pineapple contains a proteolytic enzyme known as bromelain, which
breaks down proteins. Bromelain not only breaks down the tough meat fibers
and the gelatin in Jell-O, but it also eats away at the human tongue and skin. (It is
most prevalent in the pineapple's stem.)

2. Explain the results obtained with fresh and canned pineapple (5 pts)
 Through the experiment, we discovered that fresh pineapple produced more
juice than canned pineapple. We think this occurs because fresh pineapple has
more proteins than canned pineapple, which pineapple bromelain digests, and
when the pineapple encounters the gelatin, it starts to eat away at it. Everything
becomes watery once more as the lengthy protein chains collapse.

3. If you use papaya, what enzyme in papaya will utilize gelatin as substrate? (5 pts) What
kind of enzyme  is this? (2 pts) What does it do or what is its specific action (5 pts)
 Enzyme papain is the enzyme in papaya that will utilize as a substrate. Enzyme
Papain is a proteolytic enzyme used as a meat tenderizer and as a stabilizing
agent in the brewing industry, is recovered from the latex of papaya fruit. This
kind of enzyme breaks down proteins and contains substances that might help
fight infection and heal wounds.

4. If you use agar-agar as material (or substrate), will you get the same results as in above
where you use the gelatin? Why or why not (10 pts)
 No, we don’t think we will get the same result if we substitute agar-agar for the
gelatin because, based on our research, though agar is a great substitute to
gelatin and they might serve similar purposes as gelling agents and thickening
agents in various recipes. You cannot expect the same outcome because, first, it
doesn't have the same texture. Gelatin can give a creamy texture, whereas agar
gives a firmer texture, which can result in a different outcome if used.

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