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    Biocatalysis 20142015

    Uploaded by

    INTAN ZURINA BINTI AHMAD EDIDIK
    This document contains a multiple choice quiz and structured questions about biocatalysis and enzyme inhibition. The multiple choice questions test understanding of factors that affect enzyme activity, differences between lock-and-key and induced fit hypotheses, characteristics of competitive and non-competitive inhibitors, and types of enzymes that catalyze oxidation processes. The structured questions provide graphs depicting effects of substrate concentration on reaction rate with and without inhibitors. They ask examinees to analyze and explain the graphs, draw a labeled curve for competitive inhibition, and discuss competitive, non-competitive, and irreversible inhibition in relation to enzyme structure and activity.

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    © All Rights Reserved
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    Biocatalysis 20142015

    Uploaded by

    INTAN ZURINA BINTI AHMAD EDIDIK
    17 views3 pages
    This document contains a multiple choice quiz and structured questions about biocatalysis and enzyme inhibition. The multiple choice questions test understanding of factors that affect enzyme activity, differences between lock-and-key and induced fit hypotheses, characteristics of competitive and non-competitive inhibitors, and types of enzymes that catalyze oxidation processes. The structured questions provide graphs depicting effects of substrate concentration on reaction rate with and without inhibitors. They ask examinees to analyze and explain the graphs, draw a labeled curve for competitive inhibition, and discuss competitive, non-competitive, and irreversible inhibition in relation to enzyme structure and activity.

    Original Title

    BIOCATALYSIS-20142015

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    This document contains a multiple choice quiz and structured questions about biocatalysis and enzyme inhibition. The multiple choice questions test understanding of factors that affect enzyme activity, differences between lock-and-key and induced fit hypotheses, characteristics of competitive and non-competitive inhibitors, and types of enzymes that catalyze oxidation processes. The structured questions provide graphs depicting effects of substrate concentration on reaction rate with and without inhibitors. They ask examinees to analyze and explain the graphs, draw a labeled curve for competitive inhibition, and discuss competitive, non-competitive, and irreversible inhibition in relation to enzyme structure and activity.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    17 views3 pages

    Biocatalysis 20142015

    Uploaded by

    INTAN ZURINA BINTI AHMAD EDIDIK
    This document contains a multiple choice quiz and structured questions about biocatalysis and enzyme inhibition. The multiple choice questions test understanding of factors that affect enzyme activity, differences between lock-and-key and induced fit hypotheses, characteristics of competitive and non-competitive inhibitors, and types of enzymes that catalyze oxidation processes. The structured questions provide graphs depicting effects of substrate concentration on reaction rate with and without inhibitors. They ask examinees to analyze and explain the graphs, draw a labeled curve for competitive inhibition, and discuss competitive, non-competitive, and irreversible inhibition in relation to enzyme structure and activity.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
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    of 3

    TOPIC : BIOCATALYSIS

    PART A : MULTIPLE CHOICE QUESTIONS

    1. What are the factors that affecting the enzyme activity?


    I. pH
    II. temperature
    III. presence of inhibitor

    A. I only
    B. I and II only
    C. II and II only
    D. I,II and III

    2. What is the difference between the lock and key hypothesis and the induced fit
    hypothesis?
    A. The configuration of the active site
    B. The composition of the enzyme
    C. The type of substrate
    D. The type of products formed

    3. A competitive inhibitor
    I. Has a similar structure to the normal substrate molecule
    II. Can fit into the active site of the enzyme
    III. Binds to the allosteric site

    A. I only
    B. I and II only
    C. II and III only
    D. 1, II and III

    4. Which of the following is true about coenzymes?


    I. Most are derived from the vitamin B group
    II. Do not have any prosthetic groups
    III. Are tightly bound to the enzyme

    A. I only
    B. I and II only
    C. II and III only
    D. 1, II and III
    5. Enzymes which catalyse oxidation processes include
    A. Lipase
    B. Dehydrogenase
    C. Amylase
    D. Peptidase

    PART B : STRUCTURED QUESTIONS

    1. FIGURE 2 shows the effect of increasing substrate concentration on the rate of a


    particular reaction in the present and absent of inhibitor
    Without inhibitor
    A

    Rate of reaction With non-competitive inhibitor


    B

    Substrate concentration

    i. How do graph A and B differ?


    [1 mark]
    __________________________________________________________________
    __________________________________________________________________

    ii. Explain the difference that stated in 1(i)


    [2 marks]
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    iii. Draw labeled curve to show the effect on the rate of the enzyme catalyzed
    reaction of the addition of competitive inhibitor

    [2 marks]

    iv. Explain the effect of a competitive inhibitor on the rate of enzyme activity
    [3 marks]
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________

    v. Explain briefly about irreversible inhibitor in relation to enzyme structure and


    activity.
    [2 marks]
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________

    PART C : ESSAY QUESTION

    1. a) Briefly explain the types of inhibitor


    [3 marks]
    b) Distinguish between competitive and non-competitive inhibition of enzymes.
    [7 marks]

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