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    PhysioEx Exercise 3 Activity 4

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    Alesandra Campo Sanmartín
    The document summarizes an experiment examining the role of voltage-gated sodium channels in nerve impulse propagation using the blockers TTX and lidocaine. In the control condition, action potentials were recorded at electrodes R1 and R2 with the same amplitude. Application of TTX between the electrodes blocked the response at R2 but not R1, while lidocaine blocked the response at R2 over time but not immediately. This supported that TTX and lidocaine block sodium channels, preventing action potential propagation while sparing areas where the blockers were not applied.

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    PhysioEx Exercise 3 Activity 4

    Uploaded by

    Alesandra Campo Sanmartín
    43 views5 pages
    The document summarizes an experiment examining the role of voltage-gated sodium channels in nerve impulse propagation using the blockers TTX and lidocaine. In the control condition, action potentials were recorded at electrodes R1 and R2 with the same amplitude. Application of TTX between the electrodes blocked the response at R2 but not R1, while lidocaine blocked the response at R2 over time but not immediately. This supported that TTX and lidocaine block sodium channels, preventing action potential propagation while sparing areas where the blockers were not applied.

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    The document summarizes an experiment examining the role of voltage-gated sodium channels in nerve impulse propagation using the blockers TTX and lidocaine. In the control condition, action potentials were recorded at electrodes R1 and R2 with the same amplitude. Application of TTX between the electrodes blocked the response at R2 but not R1, while lidocaine blocked the response at R2 over time but not immediately. This supported that TTX and lidocaine block sodium channels, preventing action potential propagation while sparing areas where the blockers were not applied.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    43 views5 pages

    PhysioEx Exercise 3 Activity 4

    Uploaded by

    Alesandra Campo Sanmartín
    The document summarizes an experiment examining the role of voltage-gated sodium channels in nerve impulse propagation using the blockers TTX and lidocaine. In the control condition, action potentials were recorded at electrodes R1 and R2 with the same amplitude. Application of TTX between the electrodes blocked the response at R2 but not R1, while lidocaine blocked the response at R2 over time but not immediately. This supported that TTX and lidocaine block sodium channels, preventing action potential propagation while sparing areas where the blockers were not applied.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 5

    16/3/22, 16:36 PhysioEx Exercise 3 Activity 4

    PhysioEx Lab Report


    Exercise 3: Neurophysiology of Nerve Impulses
    Activity 4: The Action Potential: Importance of Voltage-Gated Na+ Channels
    Name: práctica 1 actividad 4 ejercicio 3, G1 Date: 16 March 2022
    Session ID: session-e872bc2a-d9fa-c60f-772f-6c602abf5418

    Pre-lab Quiz Results


    You scored 100% by answering 4 out of 4 questions correctly.

    1 Voltage-gated Na+ channels are membrane channels that open

    You correctly answered: when the membrane depolarizes.

    2 When open, Na+ channels allow

    You correctly answered: Na+ ions to diffuse into the cell.

    3 Which of the following is true of an action potential?

    You correctly answered: All of these answers are correct.

    4 Which of the following can reduce the likelihood of an action potential?

    You correctly answered: All of these can reduce the likelihood of an action potential.

    Experiment Results
    Predict Questions
    1 Predict Question 1: If you apply TTX between recording electrodes R1 and R2, what effect
    will the TTX have on the action potentials at R1 and R2?

    Your answer: TTX will block the response at R1 and R2.

    2 Predict Question 2: If you apply lidocaine between recording electrodes R1 and R2, what
    effect will the lidocaine have on the action potentials at R1 and R2?

    Your answer: Lidocaine will block the response at R2 but have no effect at R1.

    Stop & Think Questions


    1 Enter the peak value of the response at R1 and R2.

    You answered: 100 μV.

    2 With a slower timescale, the appearance of the action potentials generated at R1 and R2
    will appear to
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    16/3/22, 16:36 PhysioEx Exercise 3 Activity 4

    You correctly answered: be compressed in time but have the same peak value of
    response.

    3 Why do you think TTX is not used during dental procedures?

    You correctly answered: TTX irreversibly blocks voltage-gated sodium channels in axonal
    membranes.

    Experiment Data
    Condition Stimulus Electrode Peak Peak Peak Peak Peak
    Voltage Value of Value of Value of Value of Value of
    (mV) Response Response Response Response Response
    (μV) 2 sec (μV) 4 sec (μV) 6 sec (μV) 8 sec (μV) 10
    sec
    Control 30 R1 100 100 100 100 100
    Control 30 R2 100 100 100 100 100
    TTX 30 R1 100 100 100 100 100
    TTX 30 R2 100 100 0 0 0
    Lidocaine 30 R1 100 100 100 100 100
    Lidocaine 30 R2 100 100 100 0 0

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    16/3/22, 16:36 PhysioEx Exercise 3 Activity 4

    Post-lab Quiz Results


    You scored 100% by answering 4 out of 4 questions correctly.

    1 In the control, the amplitudes of the action potentials at R1 and R2 are the same. Which
    of the following explains this?

    You correctly answered: All of these are reasonable explanations.

    2 Blocking the voltage-gated Na+ channels between R1 and R2 with TTX blocks

    You correctly answered: the propagation of the action potential from R1 to R2.

    3 When voltage-gated Na+ channels between R1 and R2 are blocked with TTX, an action
    potential is still recorded at R1 because

    You correctly answered: the voltage-gated Na+ channels between the stimulus and R1 are
    unaffected by the TTX.

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    physioex.pearson.es.ezbusc.usc.gal/exercise-3/ex03act04/ 3/5
    16/3/22, 16:36 PhysioEx Exercise 3 Activity 4

    4 Puffer fish must be prepared carefully and properly before they can be eaten. Eating
    puffer fish can cause numbness of the lips, probably because

    You correctly answered: action potentials from sensory neurons in the lips are blocked.

    Review Sheet Results


    1 What does TTX do to voltage-gated Na+ channels?

    Your answer:

    Los bloquea interrumpiendo la propagación del potencial de membrana.

    2 What does lidocaine do to voltage-gated Na+ channels? How does the effect of lidocaine
    differ from the effect of TTX?

    Your answer:

    También bloquea a estos canales, pero en comparación con la TTX su efecto es menos
    prolongado en el tiempo.

    3 A nerve is a bundle of axons, and some nerves are less sensitive to lidocaine. If a nerve,
    rather than an axon, had been used in the lidocaine experiment, the responses recorded
    at R1 and R2 would be the sum of all the action potentials (called a compound action
    potential). Would the response at R2 after lidocaine application necessarily be zero? Why
    or why not?

    Your answer:

    No necesariamente, dado que podría suceder que algunos nervios no se vieran


    afectados.

    4 Why are fewer action potentials recorded at recording electrodes R2 when TTX is applied
    between R1 and R2? How well did the results compare with your prediction?

    Your answer:

    El potencial de acción sí que pudo propagarse por R1, lo que sucede es que la aplicación
    de TTX hizo que no se pudiera propagar correctamente hacia R2, por eso se registraron
    aquí menos potenciales de acción.

    5 Why are fewer action potentials recorded at recording electrodes R2 when lidocaine is
    applied between R1 and R2? How well did the results compare with your prediction?

    Your answer:

    Sucede lo mismo que para el caso de la TTX, no se interrumpe la propagación del


    potencial de acción entre el estímulo y R1, pero se impide su propagación hacia R2.

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    16/3/22, 16:36 PhysioEx Exercise 3 Activity 4

    6 Pain-sensitive neurons (called nociceptors) conduct action potentials from the skin or
    teeth to sites in the brain involved in pain perception. Where should a dentist inject the
    lidocaine to block pain perception?

    Your answer:

    Debería aplicarse en estos mismos receptores o en sus proximidades para que así no
    pueda haber propagación del estímulo, entonces no llega a esta zona del cerebro
    encargada de interpretar esta información y no se genera la sensación de dolor.

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