Notes Alternative To Practical Biology
Notes Alternative To Practical Biology
Notes Alternative To Practical Biology
Summarized notes
5. Enzyme Activity
2H2O2 (l) → H2O (l) + O2
This reaction can be catalyzed by an enzyme (catalase) or
by a non-biological catalyst (Manganese IV oxide)
+201021291511
Dr. Omar Taha
Fats: Emulsion test; ethanol is added to mixture, and this Take two destarched potted plants.
is poured into a test tube with an equal amount of Cover both the plants with bell jars and label them as A
distilled water, +ve result = milky-white emulsion and B.
Inside A, keep NaHCO 3 (sodium bicarbonate). It produces
7. Factors of Photosynthesis CO 2.
Inside B, keep NaOH (Sodium hydroxide). It absorbs CO 2.
Keep both the set-ups in the sunlight for at least 6 hours.
7.1. Chlorophyll Is Necessary for Perform the starch test on both of the plants.
The leaves of Plant A will turn black after the starch test
Photosynthesis
The leaves of Plant B will remain orange/brown after
starch test
Take a potted plant with variegated (green and white)
leaves.
Destarch the plant by keeping it in complete darkness for
48hrs
Expose the plant to the sunlight for a few days.
Leaf boiled in water for 2 minutes to break down cell
walls,
denature enzymes and allow for easier penetration by
ethanol.
Warmed in ethanol until leaf is colourless to extract
chlorophyll,
which would mask observation
Dipped in water briefly: to soften leaf and then Leaf is
placed on a white tile and iodine is added. If starch is
7.4. Investigating what happens when
present, colour will be blue-black and if absent, it will
remain orange varying the factors affecting
photosynthesis
Light intensity: First a lamp is placed as close as possible
to the apparatus, then the experiment is repeated several
times, each times with the lamp further away from the
apparatus. Heat from the bulb is prevented by placing a
clear glass sheet between the lamp and the apparatus,
and the pond weed used is left for several minutes in
each new light intensity to allow it to adjust to new
7.2. Light Is Necessary for conditions before rate is measured.
Photosynthesis Carbon dioxide: vary the amount of hydrogen carbonate
in the solution, this supplies the plant with carbon dioxide
Destarch the plant by keeping it in darkness for 48hrs for photosynthesis
Place a stencil over part of a leaf Temperature: set up the apparatus in several different-
Place the leaf in sunlight for 4-6 hours temperature environments
Remove the stencil and test for starch
+ve result = parts which received light turn black
-ve result = parts which didn’t receive light remain
yellow/brown
+201021291511
Dr. Omar Taha
+201021291511
Dr. Omar Taha
+201021291511