Calculations ISI Capillary Electrophoresis

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300

100
50 C1 V1 Concentration
10 300 50 300
100 16.67 100
600 50 8.33 50
100 10 1.67 10

Concentration
300
100
50
10
?

m 5.04
b -23.23
Area Chart Title
85.47 600
486.00
f(x) = − 0.0182569061152863 x² + 5.07491762865754 x + 203.484114561477
487.92 500 R² = 0.926246602868149
215.85
34.19 400

300

200

100

0
0 50 100 150 200 250 300 350

Area Chart Title


487.92 600.00
486.00
f(x) = 1.31498993963783 x + 154.76615694165
215.85 500.00 R² = 0.582641773621031
34.19
85.47 400.00

300.00

200.00

100.00

0.00
0 50 100 150 200 250 300 350

Calibration curve
600.00

500.00
f(x) = 5.03580327868852 x − 23.2295081967213
400.00 R² = 0.997645974893456

300.00
Area

200.00

100.00

0.00
0 20 40 60 80 100 120
Concentration (mg/l)
100.00

0.00
0 20 40 60 80 100 120
Concentration (mg/l)
Distance Velocity Voltage
Compound Time (min)
(cm) (cm/min) applied (V)
DMSO 8 0.462 17.3160173 -20000
ASA 8 0.715 11.1888112 -20000
SA 8 0.763 10.4849279 -20000

0 120
0 120
Effective electrophoretic
mobility (cm2/v)
0
-0.010048181
-0.011

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