Calculations ISI Capillary Electrophoresis
Calculations ISI Capillary Electrophoresis
Calculations ISI Capillary Electrophoresis
100
50 C1 V1 Concentration
10 300 50 300
100 16.67 100
600 50 8.33 50
100 10 1.67 10
Concentration
300
100
50
10
?
m 5.04
b -23.23
Area Chart Title
85.47 600
486.00
f(x) = − 0.0182569061152863 x² + 5.07491762865754 x + 203.484114561477
487.92 500 R² = 0.926246602868149
215.85
34.19 400
300
200
100
0
0 50 100 150 200 250 300 350
300.00
200.00
100.00
0.00
0 50 100 150 200 250 300 350
Calibration curve
600.00
500.00
f(x) = 5.03580327868852 x − 23.2295081967213
400.00 R² = 0.997645974893456
300.00
Area
200.00
100.00
0.00
0 20 40 60 80 100 120
Concentration (mg/l)
100.00
0.00
0 20 40 60 80 100 120
Concentration (mg/l)
Distance Velocity Voltage
Compound Time (min)
(cm) (cm/min) applied (V)
DMSO 8 0.462 17.3160173 -20000
ASA 8 0.715 11.1888112 -20000
SA 8 0.763 10.4849279 -20000
0 120
0 120
Effective electrophoretic
mobility (cm2/v)
0
-0.010048181
-0.011