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    RF Short Procedure Updated

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    Dinesh Sreedharan
    The document provides instructions for performing an immunoturbidimetric test to measure RA factor levels. It details how to reconstitute and dilute the calibrator, the test programming parameters including wavelength, temperature, reagent and sample volumes, and delay and read times. It also lists the normal and linear ranges. The test procedure section shows the reagent and sample volumes used for the blank, calibrators and test samples.

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    RF Short Procedure Updated

    Uploaded by

    Dinesh Sreedharan
    6 views1 page
    The document provides instructions for performing an immunoturbidimetric test to measure RA factor levels. It details how to reconstitute and dilute the calibrator, the test programming parameters including wavelength, temperature, reagent and sample volumes, and delay and read times. It also lists the normal and linear ranges. The test procedure section shows the reagent and sample volumes used for the blank, calibrators and test samples.

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    The document provides instructions for performing an immunoturbidimetric test to measure RA factor levels. It details how to reconstitute and dilute the calibrator, the test programming parameters including wavelength, temperature, reagent and sample volumes, and delay and read times. It also lists the normal and linear ranges. The test procedure section shows the reagent and sample volumes used for the blank, calibrators and test samples.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    6 views1 page

    RF Short Procedure Updated

    Uploaded by

    Dinesh Sreedharan
    The document provides instructions for performing an immunoturbidimetric test to measure RA factor levels. It details how to reconstitute and dilute the calibrator, the test programming parameters including wavelength, temperature, reagent and sample volumes, and delay and read times. It also lists the normal and linear ranges. The test procedure section shows the reagent and sample volumes used for the blank, calibrators and test samples.

    Copyright:

    © All Rights Reserved
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    RA FACTOR - Immunoturbidimetric Method

    Reconstitute with 2mL of Distilled water


    CALIBRATOR PREPARATION :
    (Calibrator concentration is stated on vial)
    Calibrator Dilution 1 2 3 4 5
    Calibrator RF(µL) 10 25 50 75 100
    Normal Saline(µL) 90 75 50 25 0
    Factor 0.1 0.25 0.5 0.75 1

    Note : Reagents is Ready to Use

    TEST PROGRAMMING :
    Fixed Time or Two
    Mode of Reaction
    Point Kinetic
    Wavelength - Primary 650 nm(620 or 630)
    Temperature 37°C
    Working Reagent Vol 1000/ 500µL
    Sample Volume 10/ 5 µL
    Delay Time 10 seconds
    Read Time 120 seconds
    Reaction Direction Increasing
    Blank Reagent
    Unit IU/mL
    Normal Range Upto 20 IU/mL
    Linearity Up to 160 IU/mL

    TEST PROCEDURE :
    Blank Calibrator Test
    Reagent1 (µL) 800 / 400 800 / 400 800 / 400
    Reagent2 (µL) 200 / 100 200 / 100 200 / 100
    DM water (µL) *10 / 5
    Calibrator (µL) *10 /5
    Sample/Control (µL) *10 / 5

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