Bio Preservation

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Paper No.

: 03
Paper Title: FOOD MICROBIOLOGY
Module – 13: Biopreservation of foods
CONTENTS

1. Introduction

2. Biological methods for food preservation

2.1 Lactic Acid Bacteria

2.1.1 Bacteriocins from Lactic Acid Bacteria

3. Application of bacteriocins in selected foods

4. Limitations of Biopreservation process


BIOPRESERVATION
 Biopreservation is the use of natural or controlled microbiota or antimicrobials as a way of
preserving food and extending its shelf life.

 Beneficial bacteria or the fermentation products produced by these bacteria are used in
biopreservation to control spoilage and render pathogens inactive in food.

 The main purpose of the use of such practices is to restrict the number of microorganisms
present in the food.
BIOLOGICAL METHODS FOR FOOD
PRESERVATION

 Biopreservation is practiced to extend the shelf life and food safety through the use of
natural or controlled microbiota and or their antimicrobial compounds.

 The easiest forms of food biopreservation are fermentation, a process based on the
growth of microorganisms in foods, whether natural or added.

 Conventionally, a several number of foods have been protected against spoiling by


natural processes of fermentation.

 Lactic acid bacteria (starter/protective cultures), which produce organic acids and other
compounds that, in addition to antimicrobial properties, also confer unique flavours and
textures to food products.
…….BIOLOGICAL METHODS FOR FOOD PRESERVATION

Bacteriocins from Lactic Acid Bacteria

 Bacteriocins are the antimicrobial ribosomally synthesized


peptides produced by LAB and other bacteria.

 Such peptides are effective to kill only closely related


microorganisms and very sensitive in nature, they are
inactivated by proteases in the GIT.
http://en.wikipedia.org/wiki/Class_II
 The bacteriocins can effectively be used to inhibit some _bacteriocin
gram-positive bacteria, spore-forming bacteria, and food-
borne pathogens.
APPLICATION OF BACTERIOCINS
 Inoculation lactic acid bacteria in the food where
bacteriocins are produced in situ. Here, the ability of the
starter culture to grow and conditions to produce
bacteriocins in the products is very critical for its successful
application.

 Addition intact bacteriocins, which may be purified or


semi-purified as food preservatives.
http://www.freewtc.com/products/pr
 Use of a food product earlier fermented with a obiotic-toothpaste-593684-
40876.htm
bacteriocins producing strain as an ingredient in food
processing.
LIMITATIONS OF BIOPRESERVATION PROCESS

The main functional limitations for the application of bacteriocins in food preservation:

 Its relatively narrow inhibiting activity spectrum and usually active against only one target
microorganism.

 They are generally not active against gram-negative bacteria.

 The activity of bacteriocin is not stable and loss occurs when it interacts with food components by
binding with some other food components such as lipids and proteins or being degraded by
proteolytic enzymes.
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