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    Microscope Lab: The Letter "E": Purpose

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    Antonio a
    This document provides instructions for a microscopy lab where students will learn how to use a microscope and observe the letter "e" at different magnifications. The objectives are to properly use a microscope from low to high power and determine how the image changes. Students will observe a slide with the letter "e" at 40x, 100x, and 400x magnification and draw their observations. They will also answer discussion questions about microscope use and field of view size at different magnifications.

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    Microscope Lab: The Letter "E": Purpose

    Uploaded by

    Antonio a
    213 views4 pages
    This document provides instructions for a microscopy lab where students will learn how to use a microscope and observe the letter "e" at different magnifications. The objectives are to properly use a microscope from low to high power and determine how the image changes. Students will observe a slide with the letter "e" at 40x, 100x, and 400x magnification and draw their observations. They will also answer discussion questions about microscope use and field of view size at different magnifications.

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    Lab

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    This document provides instructions for a microscopy lab where students will learn how to use a microscope and observe the letter "e" at different magnifications. The objectives are to properly use a microscope from low to high power and determine how the image changes. Students will observe a slide with the letter "e" at 40x, 100x, and 400x magnification and draw their observations. They will also answer discussion questions about microscope use and field of view size at different magnifications.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    213 views4 pages

    Microscope Lab: The Letter "E": Purpose

    Uploaded by

    Antonio a
    This document provides instructions for a microscopy lab where students will learn how to use a microscope and observe the letter "e" at different magnifications. The objectives are to properly use a microscope from low to high power and determine how the image changes. Students will observe a slide with the letter "e" at 40x, 100x, and 400x magnification and draw their observations. They will also answer discussion questions about microscope use and field of view size at different magnifications.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 4

    Name _______________________________________ date _________________ per ____________

    Microscope Lab: The Letter “e”

    Purpose:
    Microscopes are only as accurate as their users. If you do not know how to use a microscope properly,
    you will have a difficult time finding specimens/cells in this unit. The letter “e” is something everyone
    should be familiar with (or at least I hope so). We all know that an “e” is round with a line connecting the
    upper half. Today, you will use that knowledge to determine how a microscope works.

    You have two objectives to fulfill by the end of this lab:


    1. Properly use and focus a microscope from a low power to a high power objective lens.
    2. Determine what happens to the image of a specimen in the microscope.

    Materials:
    • Microscope • worksheet
    • Slide • Ruler

    Procedures:
    Part I: Getting familiar with the microscope

    a. Notice the eyepiece, what is the magnifying power of the eye piece? 10 X
    b. Notice the low-power objective lens: It reads “4X lens.” It is used to view the whole slide. If
    total magnifying power of this lens is determined by multiplying the magnifying power of the
    eyepiece by the magnifying power of the objective, what is the total magnifying power of this
    objective? 40 X
    c. Notice the high-power objective lens: It reads “Holds 40X lens.” Used to view object in even
    greater detail. What is the total magnifying power of this lens? 400 X

    Part II: Focusing the letter “e”

    a. Lower the stage using the coarse adjustment knob.


    b. Place the slide of the letter “e” on the stage and stabilize it with the stage clips. Center the “e”
    the best you can on the stage.
    c. Place the lowest (ie. 4X Objective Lens) into position.
    d. Looking into the eyepiece. SLOWLY use the coarse adjustment to raise the stage closer to the
    objective lens until the object (ie. “e”) comes into focus.
    e. Once the object is seen, it may be necessary to adjust the amount of light. To increase or
    decrease contrast, rotate the iris diaphragm slightly.
    f. Use the fine adjustment knob to sharpen focus if necessary.
    g. In the space provided, draw an outline of the letter “e” as it appears through the eyepiece.

    Calculate the
    Magnification: 40X

    h. Change Magnification. Place the 10X (medium power) objective lens into position. Follow
    procedures f – h. Draw the outline of the letter “e” as it appears through the eyepiece.

    Calculate the
    Magnification: _________
    i. Lower the stage. Place the 40X objective lens into position. Follow procedures f – h. Draw the
    letter “e” as it appears through the eyepiece.

    Calculate the
    Magnification: _________

    Discussion Question: (full sentences)

    1. Was the image of “e” “right side up” or “upside down”? Upside down

    2. What happens to the microscope image when you move the microscope slide towards you?
    It looks closer you

    3. What happens to the image when you move the microscope slide to the left?
    We can’t see the “e”

    4. Why must the specimen be centered before switching to high power?

    We see a more clear image

    5. Why should you only use the fine adjust when the high-power objective is in position?

    Upside down

    6. If you placed a letter “g” under the microscope, how would the image look in the field of
    view?
    It see like a 6

    7. If a microscope has an ocular with a 5x power, and has objectives with powers of 10x
    and 50x, what is the total magnification of: (Show your math for full credit!)

    a. low power? 50x b. high power? 500x


    Bonus Lab: Determining the “Field of View”

    Procedures:
    a. Using your Microscope procedure knowledge and a plastic clear ruler, determine how many
    millimeters are in the field of view under 40X, 100X, 400X.
    b. Draw each field of view and write the number of millimeters.

    Magnification = 40X Magnification = 100X Magnification = 400X

    # of mm =________ # of mm =____________ # of mm =_________

    8. What happens to the field of view as the magnification increases?

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