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GUJARAT TECHNOLOGICAL UNIVERSITY

Bachelor of Engineering
Subject Code: 3131302
Semester – III
Subject Name: Environmental Microbiology and Bioremediation

Type of course: Engineering Science

Prerequisite: None

Rationale: Microorganisms not play a very important role in treatment and disposal of wastes but also are
responsible for spreading many diseases. Hence a working knowledge of microbiology forms a base for
other core subjects of environmental engg.

Teaching and Examination Scheme:

Teaching Scheme Credits Examination Marks Total


L T P C Theory Marks Practical Marks Marks
ESE (E) PA (M) ESE (V) PA (I)
3 0 2 4 70 30 30 20 150

Content:

Sr. No. Content Total


Hrs
1 Introduction to microbiology: 04
Scope of microbiology, Structure and classification of microbes, Role of microbes in
human life and environment, Prokaryotic cell, Cytoplasm of Eukaryotes
2 Basic methods in microbiology: 06
Microscopic methods, Techniques of sterilization, Media preparation, Isolation and
inoculation, direct observation and staining techniques, Maintenance and preservation of
cultures
3 Microscope and Microscopy: 04
Optical Microscopes and electron microscope
4 Prokaryotes and Viruses: 04
Brief description about Bacteria and Viruses and their role and importance in Environment
5 Eukaryotes: 06
Brief description about protozoa, algae and fungi and their role and importance in
Environment.
6 Microorganisms and Human diseases: 06
Diseases caused by bacteria, fungi and protozoa
7 Role of Microorganisms in Environment, Industry and Food: 06
Soil microorganisms, microorganisms in aquatic habitats, microorganisms and pollution,
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w.e.f. AY 2018-19
GUJARAT TECHNOLOGICAL UNIVERSITY
Bachelor of Engineering
Subject Code: 3131302
Microorganisms in sewage, Fermentation processes, products of industrial fermentation

8 Control of microbes: 02
Principles of control of microbes, Uses of physical agents and chemical agents.
9 Bioremediation: 04
Introduction, Fundamental principles, In-situ bioremediation of soil and Groundwater, Ex-
situ bioremediation of soil, Wastewater bioremediation, Innovative treatment technologies,
Case studies.

Suggested Specification table with Marks (Theory): (For BE only)

Distribution of Theory Marks

R Level U Level A Level N Level E Level C Level


20 20 30 0 0 0

Legends: R: Remembrance; U: Understanding; A: Application, N: Analyze and E: Evaluate C:


Create and above Levels (Revised Bloom’s Taxonomy)

Note: This specification table shall be treated as a general guideline for students and teachers. The actual
distribution of marks in the question paper may vary slightly from above table.

Reference Books:

1. Microbiology by Pelczar and Ried


2. Environmental Microbiology by Ralph Mitchell
3. Wastewater Engineering- Treatment and Reuse, Metcalf and Eddy, Inc., Revised by
Tchobanoglous, Burton and Stensel
4. Introduction to Microbiology by A.S. Rao
5. Environmental Microbiology by Manish L. Shrivastva
6. Handbook of Bioremediation Edited by Norris et al, Robert S. Kerr; Environmental Research
Laboratory.
7. Bioremediation Principles: Ewies, Ergas, Chang and Schroeder

Course Outcomes:

Sr. CO statement Marks %


No. Weightage

CO-1 Identify techniques to enumerate microbes 15

CO-2 Describe the structure of microbes & relate their role in environment 40

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w.e.f. AY 2018-19
GUJARAT TECHNOLOGICAL UNIVERSITY
Bachelor of Engineering
Subject Code: 3131302
CO-3 Compare the physical & chemical agents for control of microbes 15

CO-4 Discuss the principles of bioremediation for treatment of soil, wastewater & 30
ground water.

List of Experiments:

1. To prove that microbes are ubiquities.


2. To determine MPN of given water sample.

Major Equipment:

1. Autoclave
2. Biological Incubator
3. Monopan Balance

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w.e.f. AY 2018-19

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