Methylene Blue, 1% Aqueous

Safety Data Sheet

according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations
Date of issue: 05/31/2013 Revision date: 01/09/2018 Supersedes: 01/28/2015 Version: 1.1

SECTION 1: Identification
1.1. Identification
Product form : Mixtures
Product name : Methylene Blue, 1% Aqueous
Product code : LC16940
Other means of identification : Basic Blue 9, 1% w/v
1.2. Recommended use and restrictions on use
Use of the substance/mixture : For laboratory and manufacturing use only.
Recommended use : Laboratory chemicals
Restrictions on use : Not for food, drug or household use
1.3. Supplier
LabChem Inc
Jackson's Pointe Commerce Park Building 1000, 1010 Jackson's Pointe Court
Zelienople, PA 16063 - USA
T 412-826-5230 - F 724-473-0647
[email protected] - www.labchem.com

1.4. Emergency telephone number

Emergency number : CHEMTREC: 1-800-424-9300 or 011-703-527-3887

SECTION 2: Hazard(s) identification

2.1. Classification of the substance or mixture
GHS-US classification
Not classified

2.2. GHS Label elements, including precautionary statements

Not classified as a hazardous chemical.
Other hazards not contributing to the : None.
classification
2.4. Unknown acute toxicity (GHS US)
Not applicable

SECTION 3: Composition/Information on ingredients

3.1. Substances
Not applicable
3.2. Mixtures
Name Product identifier % GHS-US classification
Water (CAS-No.) 7732-18-5 99 Not classified
Methylene Blue (CAS-No.) 61-73-4 1 Acute Tox. 4 (Oral), H302
Aquatic Acute 2, H401

Full text of hazard classes and H-statements : see section 16

SECTION 4: First-aid measures

4.1. Description of first aid measures
First-aid measures general : Never give anything by mouth to an unconscious person. If you feel unwell, seek medical
advice (show the label where possible).
First-aid measures after inhalation : Allow victim to breathe fresh air. Allow the victim to rest.
First-aid measures after skin contact : Remove affected clothing and wash all exposed skin area with mild soap and water, followed
by warm water rinse.
First-aid measures after eye contact : Rinse immediately with plenty of water. Obtain medical attention if pain, blinking or redness
persists.
First-aid measures after ingestion : Rinse mouth. Do NOT induce vomiting. Obtain emergency medical attention.
4.2. Most important symptoms and effects (acute and delayed)
Symptoms/effects : Not expected to present a significant hazard under anticipated conditions of normal use.

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Methylene Blue, 1% Aqueous
Safety Data Sheet
according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations

Symptoms/effects after skin contact : May stain the skin.

4.3. Immediate medical attention and special treatment, if necessary
None.

SECTION 5: Fire-fighting measures

5.1. Suitable (and unsuitable) extinguishing media
Suitable extinguishing media : Foam. Dry powder. Carbon dioxide. Water spray. Sand.
Unsuitable extinguishing media : Do not use a heavy water stream.
5.2. Specific hazards arising from the chemical
Fire hazard : Not flammable.
Explosion hazard : Not applicable.
Reactivity : None.
5.3. Special protective equipment and precautions for fire-fighters
Firefighting instructions : Use water spray or fog for cooling exposed containers. Exercise caution when fighting any
chemical fire. Prevent fire-fighting water from entering environment.
Protection during firefighting : Do not enter fire area without proper protective equipment, including respiratory protection.

SECTION 6: Accidental release measures

6.1. Personal precautions, protective equipment and emergency procedures
6.1.1. For non-emergency personnel
Emergency procedures : Evacuate unnecessary personnel.

6.1.2. For emergency responders

Protective equipment : Equip cleanup crew with proper protection.
Emergency procedures : Ventilate area.
6.2. Environmental precautions
Prevent entry to sewers and public waters. Notify authorities if liquid enters sewers or public waters.
6.3. Methods and material for containment and cleaning up
Methods for cleaning up : Soak up spills with inert solids, such as clay or diatomaceous earth as soon as possible. Collect
spillage. Store away from other materials.
6.4. Reference to other sections
See Heading 8. Exposure controls and personal protection.

SECTION 7: Handling and storage

7.1. Precautions for safe handling
Precautions for safe handling : Wash hands and other exposed areas with mild soap and water before eating, drinking or
smoking and when leaving work. Provide good ventilation in process area to prevent formation
of vapor.
7.2. Conditions for safe storage, including any incompatibilities
Storage conditions : Keep container closed when not in use.
Incompatible products : Strong bases. Strong acids. Sodium hypochlorite.
Incompatible materials : Sources of ignition. Direct sunlight.

SECTION 8: Exposure controls/personal protection

8.1. Control parameters
Methylene Blue (61-73-4)
Not applicable

Water (7732-18-5)
Not applicable

8.2. Appropriate engineering controls

Appropriate engineering controls : Emergency eye wash fountains should be available in the immediate vicinity of any potential
exposure.
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Methylene Blue, 1% Aqueous
Safety Data Sheet
according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations

8.3. Individual protection measures/Personal protective equipment

Personal protective equipment:
Safety glasses.

Hand protection:
Wear protective gloves

Eye protection:
Chemical goggles or safety glasses

Respiratory protection:
Respiratory protection not required in normal
conditions

Other information:
Do not eat, drink or smoke during use.

SECTION 9: Physical and chemical properties

9.1. Information on basic physical and chemical properties
Physical state : Liquid
Color : Blue
Odor : characteristic
Odor threshold : No data available
pH : No data available
pH solution : 3 - 4.5
Melting point : No data available
Freezing point : No data available
Boiling point : No data available
Flash point : No data available
Relative evaporation rate (butyl acetate=1) : No data available
Flammability (solid, gas) : Non flammable.
Vapor pressure : No data available
Relative vapor density at 20 °C : No data available
Relative density : No data available
Specific gravity / density : 1 g/ml
Solubility : Soluble in water.
Log Pow : No data available
Auto-ignition temperature : No data available
Decomposition temperature : 190 °C
Viscosity, kinematic : No data available
Viscosity, dynamic : No data available
Explosion limits : No data available
Explosive properties : Not applicable.
Oxidizing properties : None.
9.2. Other information
No additional information available

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Methylene Blue, 1% Aqueous
Safety Data Sheet
according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations

SECTION 10: Stability and reactivity

10.1. Reactivity
None.
10.2. Chemical stability
Not established.
10.3. Possibility of hazardous reactions
Not established.
10.4. Conditions to avoid
Direct sunlight. Extremely high or low temperatures.
10.5. Incompatible materials
Strong acids. Strong bases.
10.6. Hazardous decomposition products
Nitrogen oxides. Carbon monoxide. Carbon dioxide. Sulfur compounds.

SECTION 11: Toxicological information

11.1. Information on toxicological effects

Likely routes of exposure : Skin and eye contact

Acute toxicity : Not classified

Methylene Blue (61-73-4)

LD50 oral rat 1180 mg/kg (Rat)
ATE US (oral) 1180 mg/kg body weight
Water (7732-18-5)
LD50 oral rat ≥ 90000 mg/kg
ATE US (oral) 90000 mg/kg body weight
Skin corrosion/irritation : Not classified
Serious eye damage/irritation : Not classified
Respiratory or skin sensitization : Not classified
Germ cell mutagenicity : Not classified
Based on available data, the classification criteria are not met
Carcinogenicity : Not classified

Reproductive toxicity : Not classified

Based on available data, the classification criteria are not met
Specific target organ toxicity – single exposure : Not classified

Specific target organ toxicity – repeated : Not classified

exposure

Aspiration hazard : Not classified

Potential Adverse human health effects and : Based on available data, the classification criteria are not met.
symptoms
Symptoms/effects after skin contact : May stain the skin.

SECTION 12: Ecological information

12.1. Toxicity

Methylene Blue (61-73-4)

EC50 Daphnia 1 2.26 mg/l (EC50; 48 h)
LC50 fish 2 18 mg/l (LC50; 96 h)

12.2. Persistence and degradability

Methylene Blue, 1% Aqueous
Persistence and degradability Not established.

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Methylene Blue, 1% Aqueous
Safety Data Sheet
according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations

Methylene Blue (61-73-4)

Persistence and degradability Biodegradability in water: no data available. Photodegradation in the air.
Water (7732-18-5)
Persistence and degradability Not established.

12.3. Bioaccumulative potential

Methylene Blue, 1% Aqueous
Bioaccumulative potential Not established.
Methylene Blue (61-73-4)
Log Pow 5.85 (Estimated value)
Bioaccumulative potential Not bioaccumulative.
Water (7732-18-5)
Bioaccumulative potential Not established.

12.4. Mobility in soil

No additional information available

12.5. Other adverse effects

Effect on the global warming : No known effects from this product.

GWPmix comment : No known effects from this product.

Other information : Avoid release to the environment.

SECTION 13: Disposal considerations

13.1. Disposal methods
Waste disposal recommendations : Dispose in a safe manner in accordance with local/national regulations.
Ecology - waste materials : Avoid release to the environment.

SECTION 14: Transport information

Department of Transportation (DOT)
In accordance with DOT
Not regulated

SECTION 15: Regulatory information

15.1. US Federal regulations

All components of this product are listed, or excluded from listing, on the United States Environmental Protection Agency Toxic
Substances Control Act (TSCA) inventory

15.2. International regulations

CANADA
Methylene Blue (61-73-4)
Listed on the Canadian DSL (Domestic Substances List)

EU-Regulations
No additional information available

National regulations
Methylene Blue (61-73-4)
Listed on the Canadian IDL (Ingredient Disclosure List)

15.3. US State regulations

California Proposition 65 - This product does not contain any substances known to the state of California to cause cancer,
developmental and/or reproductive harm

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Methylene Blue, 1% Aqueous
Safety Data Sheet
according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations

SECTION 16: Other information

Revision date : 01/09/2018
Other information : None.
Full text of H-phrases: see section 16:
--- H302 Harmful if swallowed
---
--- H401 Toxic to aquatic life
---
NFPA health hazard : 1 - Materials that, under emergency conditions, can cause
significant irritation.
NFPA fire hazard : 0 - Materials that will not burn under typical dire conditions,
including intrinsically noncombustible materials such as
concrete, stone, and sand.
NFPA reactivity : 0 - Material that in themselves are normally stable, even
under fire conditions.

Hazard Rating
Health : 1 Slight Hazard - Irritation or minor reversible injury possible
Flammability : 0 Minimal Hazard - Materials that will not burn
Physical : 0 Minimal Hazard - Materials that are normally stable, even under fire conditions, and will NOT
react with water, polymerize, decompose, condense, or self-react. Non-Explosives.
Personal protection : B
B - Safety glasses, Gloves

SDS US LabChem

Information in this SDS is from available published sources and is believed to be accurate. No warranty, express or implied, is made and LabChem Inc assumes no liability resulting from the use of this
SDS. The user must determine suitability of this information for his application.

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