RESEARCH ARTICLE

Factors associated with newborn care

knowledge and practices in the upper
Himalayas
Devendra Raj Singh ID1,2*, Chloe M. Harvey3, Pushpalata Bohara1, Dhirendra Nath ID2,
Sunita Singh4, Sylvia Szabo3,5, Kshitij Karki1,6
1 Department of Public Health, Asian College for Advance Studies, Purbanchal University, Lalitpur, Nepal,
2 Research and Innovation Section, Southeast Asia Development Actions Network (SADAN), Lalitpur, Nepal,
3 Department of Social Statistics and Demography, University of Southampton, Southampton, England,
United Kingdom, 4 Sukraraj Tropical & Infectious Disease Hospital, Ministry of Health & Population,
a1111111111 Kathmandu, Nepal, 5 Department of Development and Sustainability, Asian Institute of Technology, Pathum
a1111111111 Thani, Thailand, 6 Program and Research Department, Group for Technical Assistance, Lalitpur, Nepal
a1111111111
a1111111111 * [email protected]
a1111111111

Abstract

OPEN ACCESS Background

Citation: Singh DR, Harvey CM, Bohara P, Nath D,
Globally, neonatal deaths remain a major public health challenge and account for the major-
Singh S, Szabo S, et al. (2019) Factors associated
with newborn care knowledge and practices in the ity of deaths occurring among children under five years of age. Despite Nepal’s significant
upper Himalayas. PLoS ONE 14(9): e0222582. achievements in meeting the maternal and child health targets of the Millennium Develop-
https://doi.org/10.1371/journal.pone.0222582 ment Goals, an estimated 23,000 Nepalese children under five years die every year, with
Editor: Charles A. Ameh, Liverpool School of three out of five babies dying within the first 28 days of life. This study therefore aimed to
Tropical Medicine, UNITED KINGDOM examine the level of knowledge and practices of newborn care among Nepalese mothers in
Received: November 27, 2018 the upper Himalayas and the factors associated with these.
Accepted: September 2, 2019
Materials and methods
Published: September 16, 2019
A community based cross-sectional study was conducted among 302 randomly selected
Copyright: © 2019 Singh et al. This is an open
mothers with children under two years of age in Tripurasundari Municipality of Dolpa district,
access article distributed under the terms of the
Creative Commons Attribution License, which an upper Himalayan region of Nepal. Mothers were interviewed using semi-structured ques-
permits unrestricted use, distribution, and tionnaires. Mean score for knowledge and Bloom’s criteria for practice were considered to
reproduction in any medium, provided the original categorize newborn care knowledge and practices. Multivariate logistic regression was
author and source are credited.
used to identify factors associated with the newborn care knowledge and practices.
Data Availability Statement: All data are within the
paper and its Supporting Information files.
Results
Funding: The authors received no funding for this
In this study, 147 (48.7%) of the mothers were found to have inadequate knowledge of new-
work.
born care, while 102 (33.8%) mothers had reported unsatisfactory newborn care practices.
Competing interests: The authors have declared
Mothers with at least secondary level of formal education were more likely to possess ade-
that no competing interests exist.
quate newborn care knowledge compared to mothers who never attended school (AOR
Abbreviations: ANC, Antenatal Care; AOR,
4.93 at 95% CI 1.82–13.33). Mothers whose first pregnancy occurred between the ages of
Adjusted Odds Ratio; CB-NCP, Community-Based
Newborn Care Program; CBS, Center Bureau of 20–24 years (AOR 3.89 at 95% CI 1.81–8.37) were also more likely to possess adequate
Statistics; COR, Crude Odds Ratio; FCHVs, Female newborn care knowledge, compared to mothers with a younger age at first pregnancy.

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 1 / 18

 Newborn care knowledge and practices in the upper Himalayas

Community Health Volunteers; PNC, Postnatal Furthermore, mothers who had completed at least four ANC visits (AOR 2.89 at 95% CI
Care; SDGs, Sustainable Development Goals. 1.04–7.96), mothers who had completed three PNC visits (AOR 2.79 at 95% CI 1.16–6.72)
and mothers who reported that their nearest health facility was less than one hour (30–59
minutes) walking distance (AOR 3.66 at 95% CI 1.43–9.33) had higher odds of having ade-
quate newborn care knowledge. Similarly, mothers whose household monthly income was
more than $100 (AOR 4.17 at 95% CI 1.75–9.69), mothers who had completed three PNC
visits (AOR 3.27 at 95% CI 1.16–9.20) and mothers with adequate newborn care knowledge
(AOR 15.35 at 95% CI 5.82–40.47) were found to be more likely to practice a satisfactory
level of newborn care practices in adjusted analysis.

Conclusion
The study revealed high prevalence of inadequate newborn care and knowledge amongst
mothers in upper Himalayan dwellings. Approximately one third of all interviewed mothers
practiced suboptimal newborn care. The results indicate an urgent need to increase aware-
ness of neonatal services available to mothers and to prioritize investments by local govern-
ments in neonatal health services, in order to improve accessibility and quality of care for
mothers and newborns.

Background
Globally, neonatal mortality remains a major public health challenge and accounts for approx-
imately half of deaths occurring among children under five years of age[1]. The majority of
these neonatal deaths occur in the first seven days of life due to preventable medical conditions
[2,3]. In 2016 alone, 2.6 million children died in the first month of life, an estimated 7,000 neo-
natal deaths occurring every day worldwide[1,3]. Approximately 99% of neonatal deaths occur
in resource poor settings [4] which equates to a mortality risk which is six times higher for
babies born in developing countries compared to those born in developed countries [5]. Pre-
term birth, severe infections, asphyxia and neonatal tetanus are the major direct causes of
global neonatal deaths [4]. Therefore, effective implementation of World Health Organization
(WHO) recommendations for essential newborn care is key to newborn survival and includes
early initiation of breast-feeding, cord care, eye care, thermoregulation, management of
asphyxia, recognition of neonate danger signs, immunization and care of low birth weight neo-
nates[6].
Although Nepal is one of the few developing nations to achieve all targets under Millen-
nium Development Goal (MDG) 4 [7], an estimated 23,000 Nepalese children under-five years
of age still die every year, with three out of five newborns dying within the first 28 days of life
[8]. According to the most recent 2016 Nepal Demographic and Health Survey (NDHS), the
neonatal mortality rate decreased at a much slower pace to 21 deaths per 1,000 live births [9].
The geographical differences in neonatal deaths range from 15 to 41 neonate deaths per 1,000
live births[9]. Due to the fact that many geographical areas in Nepal are hard to reach, the gov-
ernment has implemented community-based service delivery approaches such as the Commu-
nity-Based Newborn Care Program (CB-NCP) in 2007 and a Community Based Integrated
Management of Newborn and Childhood Illness (CB-IMNCI) in 2015 to improve child health
across the country. [10]. To further accelerate the progress, the Government of Nepal has also
recently endorsed Nepal’s Every Newborn Action Plan-2016 (NENAP) with the objective to
implement cost effective and evidence based newborn care to meet SDG target 3.2 [11].

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 2 / 18

 Newborn care knowledge and practices in the upper Himalayas

Indicators of child health vary extensively across different provinces and ecological zones in
Nepal [12]. In a developing context such as Nepal, it is important to identify the socio-demo-
graphic factors and maternal health practices associated with neonatal mortality, in order to
target child health interventions at mothers and children who are considered at risk. In Nepal,
knowledge of newborn care and use of health facilities varies between the geographical and
ecological regions, due to the social and cultural belief systems of different ethnic groups and
inequalities in health care coverage[9,12–14]. Forty one percent of total deliveries occur at
home, often in an animal shed or separate, congested room, which exacerbates the risk of
infection transmission to both mother and newborn[9,15,16]. Moreover, several studies have
highlighted poor child health status and elevated neonatal mortality in the upper Himalaya
region of Nepal, despite the implementation of several newborn and maternal health policies
by the government[8,9,17]. However, very few studies thus far have explored maternal new-
born care knowledge and variations in practices by different ecological regions in Nepal. The
objectives of this study were (1) to examine knowledge on newborn care among mothers and
practices on newborn care; and (2) to identify the factors associated with newborn care knowl-
edge and practices in Tripurasundari Municipality of Dolpa district, an upper Himalayan
region.

Materials and methods

Study design and setting
A community based cross-sectional study was conducted between November 2017 and Febru-
ary 2018, in Dolpa district in the upper Himalayan region of Nepal; situated in Karnali prov-
ince with an altitude of over 5000 meters. The district is bordered by China on the North and
has very limited roadway links, infrastructure and health care facilities. The district is an exam-
ple of extreme remoteness with very low literacy, poor socio-economic status, high prevalence
of child marriage, high prevalence of child malnutrition, low life expectancy, low institutional
delivery, and limited access to basic facilities among the majority of the population[9,17].
Dolpa district is divided into two municipalities and six rural municipalities. Tripurasun-
dari municipality was randomly selected for the study site (Fig 1). According to the National
population and housing census 2011, conducted by the Central Bureau of Statistics, Tripura-
sundari had a total population of 10,104 (4966 males and 5138 females) of which the popula-
tion of reproductive age (15–49 years) was 6604 (4452 males and 2,152 females)[18].

Sample and exclusion criteria

Sample size was calculated by using single population proportion formula for finite population
(n = [Np(1-p)]/ [(d2/Z21-α/2� (N-1)+p� (1-p)]). A total of 2,152 female population of reproduc-
tive age group (15–49) were used as the finite population (N) [18].The proportion (p) was con-
sidered 69% i.e. using antibiotic ointment (Chlorhexidine) applied on the stump of the
umbilical cord within an hour of cord being cut [9]; absolute precision (d) of 5%, and standard
normal deviation (Z) at confidence limit of 95%. The sample was adjusted to 314 with addition
of 10% non-response rate to the calculated sample size.
Mothers with at least one child aged less than two years old were considered eligible partici-
pants for this study. Mothers who were admitted to a health facility for delivery, those with
hearing and speech disabilities, and who were not present at home on the day of data collection
were excluded from the study.
A systematic random sampling method was applied to select the required sample from a list
of households with mothers who had at least one child younger than two years old. This list
was obtained from local Female Community Health Volunteers (FCHVs). Within each local

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 3 / 18

 Newborn care knowledge and practices in the upper Himalayas

Fig 1. Location of Tripurasundari Municipality. The figure shows the location of study sites (a) Nepal. (b) Dolpa
District. (c) Tripurasundari Municipality.
https://doi.org/10.1371/journal.pone.0222582.g001

administrative unit, there is at least one FCHV, who work voluntarily to advocate for optimal
health practices among mothers and the community, to promote safe motherhood, good child
health and appropriate family planning practices[19]. Households were selected with a sam-
pling interval of two which was calculated from the list of 613 eligible households. Only one
eligible participant was selected from each household. Therefore, the total number of house-
holds in the study was equal to the total number of mothers who were selected for the study. If
more than one eligible participant was present in the same household then only one partici-
pant was selected using a lottery method. A total of twelve participants refused to provide com-
plete information during the survey and thus, information from a total of 302 participants was
included in the final analysis.

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 4 / 18

 Newborn care knowledge and practices in the upper Himalayas

Study instruments and variables

A semi-structured questionnaire was prepared by reviewing relevant literature including govern-
ment policy documents and WHO guidelines. The survey questionnaire was arranged into two
sections. The first section recorded the socio-demographic characteristics of participants and the
second section collected information on mothers’ knowledge and practices of essential newborn
care. Socio-demographic characteristics were captured through a structured questionnaire. The
information collected in this section of the questionnaire included participants’ age, age at mar-
riage, age at first pregnancy, ethnic group, education level, family type (nuclear or joint family),
number of children, age of the youngest child, household monthly income, main sources of
income, distance to health facility, maternal history of antenatal check-up, postnatal check-up
for mother and baby, place of last delivery, maternal tobacco and alcohol consumption.
A semi-structured questionnaire was developed to assess a mother’s knowledge regarding
newborn care and practices. The questions concerning newborn care knowledge and practices
were developed based on WHO guidelines for essential newborn care. A total of 14 knowledge
related questions were asked to respondents to assess the level of newborn care knowledge of
mothers. The knowledge assessment questionnaire encompassed mother’s knowledge regard-
ing the neonatal period, essential components of newborn care, recognition of neonate danger
signs, causes of danger signs, safe cord caring practice, neonatal eye infection, early initiation
of breastfeeding, exclusive breastfeeding, neonatal sleep patterns, neonatal vaccines, minimum
number of neonate postnatal check-up, and Kangaroo Mother Care (KMC) for low birth
weight neonates. A total of 10 questions were asked to mothers in order to assess newborn care
practices. The newborn care practices questionnaire collected information on colostrum feed-
ing, initiation of breastfeeding within one hour of birth, immediate wiping and wrapping of
baby with clean, soft cloths, umbilical cord cutting practice, cord caring, skin to skin contact,
delayed bathing, minimum number of postnatal check-up, received neonatal vaccination and
exclusive breastfeeding.
To ensure the quality of data, the English version of the questionnaire was translated into
Nepali language and the questionnaires were then pretested in Thuli Bheri Municipality of
Dolpa district. Further corrections and required modifications of the pretested questionnaires
were done prior to implementation of the survey.

Data collection
Face to face interviews were conducted in the respondents’ households. Participants were
interviewed using semi-structured, pre-tested questionnaires. The data were collected by five
undergraduate public health students, who were fluent in Nepali language, were local residents
of the survey district, and were involved in the study from the initial planning through to the
data collection period.
The interviewers were also provided with three days of training on application of tools,
sample selection, ethical considerations and techniques of data collection prior to conducting
the survey. The average duration of each interview was approximately 45 minutes, resulting in
four completed interviews per day for each enumerator over a period of sixteen days. The col-
lected data were reviewed and validated daily to ensure the completeness and quality.

Ethical statements
The study was conducted only after obtaining ethical clearance from the Ethical Review Board
(ERB) of the Nepal Health Research Council (ERB Reg. no: 1072/2017) and District Health
office of Dolpa. All the participants were informed about the purpose of the study and verbal
with written informed consent was obtained from every participant prior to the interview.

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 5 / 18

 Newborn care knowledge and practices in the upper Himalayas

Informed consent documents were read to the participants and their literate representative in
the case of illiterate participants. After verbal informed consent, participants and their repre-
sentative’ signatures were obtained on the consent form. Collected data was anonymized and
the information obtained from the participants was kept strictly confidential.

Statistical analysis
The data were entered into EpiData software v3.1 and transferred into IBM SPSS 21 (Chicago,
IL, USA) for statistical analysis. A score method was applied to analyze the responses of knowl-
edge related questions. Every correct answer (consistent with WHO essential newborn care
guidelines) received a score of 1 mark and an incorrect answer (inconsistent with WHO essen-
tial newborn care guidelines) or a “don’t know” response received a score of 0. The mean score
of the responses was calculated from a total of 14 newborn care knowledge related questions to
use as a cut-off point to differentiate between mothers having adequate and inadequate new-
born care knowledge. Mothers scoring equal to or above the average score were considered to
have adequate knowledge and those scoring below the average score were considered to have
an inadequate level of knowledge regarding newborn care[20]. Similarly, a total of 10 questions
were asked to assess practice of newborn care where the mean score was 6.2. Considering
mean score and Bloom’s cut off points[21,22], mothers who practiced at least 6 good practices
out of 10 were considered to be implementing satisfactory practices of newborn care (i.e. �
60% correct practices) and those who did not practiced at least 6 good practices were consid-
ered to be practicing an unsatisfactory level (i.e. < 60% correct practices) of newborn care.
Descriptive statistics were performed for the outcome and all explanatory variables to explore
the characteristics of the chosen sample. Bivariate analysis was conducted between the two out-
come variables pertaining to maternal knowledge of newborn care and newborn practices and
the explanatory variables of interest, using chi-square tests of association or t-tests.
Binary logistic regression was employed to examine presupposed associations between
mothers’ newborn care knowledge and socio-demographic characteristics, such as respon-
dents age, age at marriage, age at first pregnancy, family type, education status, family monthly
income, ANC check up, PNC check up, place of last delivery, and distance to reach the nearest
health facility. The regression analysis was performed with the following dichotomous depen-
dent variable: adequate versus inadequate knowledge and satisfactory versus unsatisfactory
newborn care practices. Hosmer-Lemeshow tests were used to measure the goodness of fit of
the models. All the significant predictor variables with a statistically significant p-value
(<0.05) in univariate logistic regression analyses were included in the multivariate logistic
regression models. The final results were reported using adjusted odds ratio (AOR) with 95%
confidence intervals (CI).

Results
Demographic and socio-economic characteristics
Table 1 presents the demographic and socio-economic characteristics of mothers compared by
their level of newborn care knowledge. A total of 302 (96.18%) mothers were included in the
final analysis from the total 314 interviewed mothers. A total of 12 (3.82%) mothers refused to
provide complete information during the interview and thus they were excluded from the final
analyses. The mean ages of the study participants and that of their youngest child were 24.0 (±
3.6 SD) years and 12.3 (± 7.5 SD) months respectively. Similarly, mothers’ mean age at mar-
riage was 17.8 (± 2.2 SD) years and the mean age at first pregnancy was 19.1 (±2.1 SD) years.
Only 50.7% of households had a combined income over $100 USD per month. The average
family monthly income reported by the study subjects was $120.1 (± $68.9 SD) USD per

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 6 / 18

 Newborn care knowledge and practices in the upper Himalayas

Table 1. Demographic and socioeconomic characteristics of mothers by newborn knowledge category.

Variables Total Sample Newborn care knowledge P-value
N = 302 Inadequate Adequate
n = 147 n = 155
n(%) n(%) n(%)
Age category 0.071
15–20 56 (18.5) 33(22.4) 23(14.8)
21–25 132(43.7) 58(39.5) 74(47.7)
26–30 99(32.8) 52(35.4) 47(30.3)
31–35 15(5.0) 4(2.7) 11(7.1)
Mean ± SD 24.0 ± 3.6 23.6±3.4 24.3±3.7 0.082a
Ethnicity 0.001�
Upper Caste 153(50.7) 58(39.5) 95(61.3)
Janajati 38(12.6) 21(14.3) 17(11.0)
Dalit 111(36.8) 68(46.3) 43(27.7)
Education status <0.001�
Never attended school 94 (31.1) 65(44.2) 29(18.7)
Primary education 103(34.1) 53(36.1) 50(32.3)
Secondary education 62(20.5) 18(12.2) 44(28.4)
Higher secondary education and above 43(14.2) 11(7.5) 32(20.6)
Type of Family 0.845
Nuclear 210 (69.5) 103(70.1) 107(69.0)
Joint 92(30.5) 44(29.9) 48(31.0)
Occupation <0.001�
Business 34 (11.3) 3(2.0) 31(20.0)
Service 8(2.6) 3(2.0) 5(3.2)
Labor 32(10.6) 19(12.9) 13(8.4)
Home maker 48(15.9) 24(16.3) 24(15.5)
Agriculture 180(59.6) 98(66.7) 82(52.9)
Monthly income (USD) <0.001�
� 100 149(49.3) 91(61.9) 58(37.4)
>100 153(50.7) 56(38.1) 97(62.6)
Mean ± SD ($) 120.1 ± 68.9 103.2±60.0 136±72.9 <0.001a�
Age at marriage (years) 0.374
<15 Years 25(8.3) 12(8.2) 13(8.4)
16–20 247(81.8) 124(84.4) 123(79.4)
21–25 30(9.9) 11(7.5) 19(12.3)
(Mean ± SD) 17.8± 2.2 17.3±1.9 18.3±2.3 <0.001a�
Age at first pregnancy <0.001�
(Years)
15–19 196(64.9) 121(82.3) 75(48.4)
20–24 90(29.8) 20(13.6) 70(45.2)
25–29 16(5.3) 6(4.1) 10(6.5)
Mean ± SD 19.1±2.1 18.5±1.8 19.6±2.3 <0.001a�
Age of last baby (months) 12.3± 7.5 12.3±7.2 12.3±7.8 0.964a
Mean ± SD
Number of child <0.001�
One 104(34.4) 61(41.5) 43(27.7)
Two 133(44.0) 47(32.0) 86(55.5)
Three and above 65(21.5) 39(26.5) 26(16.8)
(Continued )

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 7 / 18

 Newborn care knowledge and practices in the upper Himalayas

Table 1. (Continued)

Variables Total Sample Newborn care knowledge P-value

N = 302 Inadequate Adequate
n = 147 n = 155
n(%) n(%) n(%)
Antenatal care visit <0.001�
One 30(9.6) 18(12.2) 12(7.7)
Two 56(18.5) 38(25.9) 18(11.6)
Three 46(15.2) 32(21.8) 14(9.0)
Four and above 170(56.3) 59(40.1) 111(71.6)
Postnatal care visit <0.001�
No visit 127(42.1) 91(61.9) 36(23.2)
1–2 times 92(30.5) 29(19.7) 63(40.6)
3 times and above 83(27.5) 27(18.4) 56(36.1)
Place of delivery 0.001�
Home 180(59.6) 102(69.4) 78(50.3)
Health facility 122(40.4) 45(30.6) 77(49.7)
Distance of heath facility <0.001�
(Minutes)
<30 162(53.6) 63(42.9) 99(63.9)
�30–59 87(28.8) 44(29.9) 43(27.7)
�60 53(17.5) 40(27.2) 13(8.4)
Mean ± SD 38.7±33.5 44.1±36.7 33.6±29.5 0.007a�
Smoking 0.893
No 227(75.2) 111(75.5) 116(74.8)
Yes 75(24.8) 36(24.5) 39(25.2)
Alcohol intake habit 0.097
No 164(54.3) 87(59.2) 77(49.7)
Yes 138(45.7) 60(40.8) 78(50.3)
a
P-value from independent t-test; and others are from Chi-Square test.
�
P-value <0.05.
SD, standard deviation.

https://doi.org/10.1371/journal.pone.0222582.t001

month. The proportion of mothers who had never attended school was 31%, a further 34.1%
had primary (grades 1–5) education, 20.5% had achieved secondary (grades 6–10) education
and only 14.2% were found to have attained higher secondary (grades 11–12) or university
level education. More than half of the study participants were from upper castes (50.7%) fol-
lowed by Dalit (36.8%) and Janajati (12.6%). Agriculture/farming was the major occupation
for the majority of the participants. The average walking distance to reach a health facility was
38.7 (±33.5 SD) minutes, as reported by the mothers.
Only 56.3% of the interviewed mothers were found to have completed four Antenatal Care
(ANC) check-ups and 27.5% of mothers had completed three Postnatal Care (PNC) check-ups
during their last pregnancy. Moreover, only 40.4% of total mothers had delivered their last
child in a health facility.

Mothers’ newborns care knowledge and associated factors

The mean score of newborn care knowledge among the total sample was 7.36 (±2.88 SD)
(Table 2). The maximum score obtained was 12.15 and the minimum score was 2.00 out of a

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 8 / 18

 Newborn care knowledge and practices in the upper Himalayas

Table 2. Newborn care knowledge among mothers and newborn care practices in upper Himalaya.
Newborn care knowledge Correct response
n (%)
Knowledge about newborn or neonate period 155(51.3)
Knowledge on at least 4 major component of immediate essential newborn care (immediate 132(43.7)�
wipe and warping baby, hygienic cord cutting practice, skin to skin contact of baby and early
initiation of breast feeding to newborn).
Knowledge on least 4 benefits of newborn essential care (prevents newborn from 66(21.9)�
hypothermia, hypoglycemia, infections and pneumonia).
Knowledge on at least 5 major danger neonatal signs 80(26.5)�
(Neonate unable to feed, unconsciousness, severe chest in drawing, convulsion, and fast
breathing)
Knowledge on at least 5 causes of neonate danger signs. 101(33.4)�
(Hypothermia, birth asphyxia, low birth weight, neonatal jaundice, and infections).
Knowledge on safe cord cutting practice 146(48.3)
Knowledge on cord caring practice (i.e. use of chlorexidine gel) 247(81.8)
Knowledge on at least 3 neonate eye infection danger signs (eye discharge, reddening of eye 156(51.7)�
and swollen eye).
Knowledge on exclusive breast-feeding practice 214(70.9)
Knowledge of frequency of breast-feeding per day for newborn. 56(18.5)
Knowledge on requirement of minimum sleep hours for newborn. 89(29.5)
Knowledge on neonatal vaccine 258(85.4)
Knowledge on minimum number of postnatal checks ups for newborn. 76(25.2)
Knowledge on caring low birth weight newborn care. (Kangaroo mother care, early and 159(52.6)
frequent breast feeding and frequent consultation with clinician).
Mean knowledge score (Mean ± SD) = 7.36 ±2.88
Level of newborn care Knowledge Inadequate (<mean knowledge score) 147(48.7)
Adequate (� mean knowledge score) 155(51.3)
Newborn care practices
Colostrum feeding 235(77.8)
Initiation of breast feeding within one hour 203(67.2)
Umbilical Cord cutting practice
New/sterile blade used from hygienic delivery kit 259(85.8)
Sickle (Hasiya) 41(13.6)
Used non sterile blade 2 (0.7)
Cord caring practice
Chlorhexidine gel/antibiotic ointment applied to cord stump 189(62.2)
Butter/Oil (cooking oil) 45(14.9)
Turmeric powder 36(11.9)
Do not apply anything 32(10.6)
Wrapping newborn with soft and clean cloths/towel 262(86.8)
Practice of skin to skin contact 116(38.4)
Practice of delayed bathing (after 24 hours) 140(46.4)
Baby had at least 3 PNC checks up completed 83(27.5)
Newborn received BCG vaccine 298(98.7)
Practice of exclusive breast feeding 164(54.3)
Level of newborn care practice Unsatisfactory practices (<60% correct practices) 102(33.8)
Satisfactory practices (�60% correct practices) 200(66.2)
�
Multiple responses

https://doi.org/10.1371/journal.pone.0222582.t002

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 9 / 18

 Newborn care knowledge and practices in the upper Himalayas

total 14 points. Considering the mean score of knowledge as the assigned cut-off point, 48.7%
of mothers were found to have an inadequate level of newborn care knowledge and 51% of
mothers were found to have an adequate level of newborn care knowledge. Further, chi-square
and t-tests were applied where necessary to observe the significant associations between socio-
demographic characteristics and maternal level of knowledge regarding newborn care.
Results of the univariate regression analyses (Table 3) suggested that mothers’ level of new-
born care knowledge was significantly associated with mothers’ age at first pregnancy, ethnic
group, education status, occupation, family monthly income, number of children, ANC visit,
PNC visit, place of last delivery and distance to nearest health facility. However, the multivari-
ate regression model (Table 3) revealed that mothers who were 20–24 years old at first preg-
nancy were three times more likely to demonstrate an adequate level of knowledge of newborn
care (AOR 3.89 at 95% CI 1.81–8.37), compared to mothers who first became pregnant before
20 years of age. Adequate maternal newborn knowledge was also found to be higher among
women who had attended school and the strength of this association was greatest for women
who completed at least higher secondary education (AOR = 5.92 at 95% CI 1.81–19.33).
Mothers who had more than one child were more likely to have adequate knowledge, com-
pared to mothers with only one child: mothers with a second child (AOR 2.65 at 95% CI 1.26–
5.56), mothers with three or more children (AOR 2.27 at 95% CI 0.92–5.59). In terms of the
health care utilization, mothers who had completed four ANC visits (AOR 2.89 at 95% CI
1.04–7.96) or three PNC visit (AOR 2.79 at 95% CI 1.16–6.72) during their last pregnancy
were two times more likely to possess an adequate level of newborn care knowledge, compared
to the mothers who had not completed four ANC or three PNC visits during their last preg-
nancy. Moreover, mothers whose nearest health facility was within a walking distance of 30–
59 minutes were more than three times as likely to demonstrate adequate newborn care knowl-
edge (AOR 3.66 at 95% CI 1.43–9.33), compared to those who had to walk for more than an
hour in order to reach their nearest health facility.

Newborn care practices and associated factors

The study showed that 200 (66.2%) of mothers had practiced at least six out of 10 good new-
born care practices (satisfactory practices) and 102 (33.8%) of mothers had practiced less than
six out of 10 good newborn care practices (unsatisfactory practices) (Table 2). In a univariate
analyses (Table 4), the independent variables observed to have a significant association with
mothers’ satisfactory level of practice were mothers age, age at first pregnancy, education sta-
tus, monthly family income, number of children, distance to nearest health facility, ANC visit,
PNC visit, place of last delivery, and newborn care knowledge. However, the multivariate logis-
tic regression model (Table 4) revealed that only mother’s monthly family income, PNC visit
and newborn care knowledge were significant predictors of satisfactory level of newborn care
practices. Mothers whose family income was more than $100 USD per month (AOR 4.17 at
95% CI 1.75–9.69), completed three postnatal visits (AOR 3.27 at 95% CI 1.16–9.20) and had
adequate newborn care knowledge (AOR 15.35 at 95% CI 5.82–40.47) were found to be more
likely to practice satisfactory level of newborn care practices.

Discussion
This study aimed to examine factors associated with knowledge of newborn care and newborn
care practices in the remote upper Himalayas of Nepal. The main findings from this study
showed that more than half (51.3%) of the mothers demonstrated an adequate level of new-
born care knowledge. Similarly, a study conducted in Ethiopia found that 36.1% of postnatal
mothers had adequate knowledge of newborn care with a 75% cut-off point [23], whereas

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 10 / 18

 Newborn care knowledge and practices in the upper Himalayas

Table 3. Factors associated with adequate maternal newborn care knowledge among mothers with child less than
two years in Dolpa district, Nepal.
Variables UOR (95%CI) AOR (95%CI)
Age at marriage (Years)
<15 Years 1 -
16–20 0.91(0.40–2.08) -
21–25 1.59(0.54–4.69) -
Age at first pregnancy (Years)
15–19 1 1
20–24 5.64(3.17–10.02)� 3.89(1.81–8.37)�
25–29 2.68(0.93–7.70) 2.07(0.48–8.88)
Ethnicity
Upper Caste 1.28(0.60–2.69) 0.96(0.30–3.05)
Janajati 2.59(1.56–4.28)� 2.34(1.13–4.84)�
Dalit 1 1
Education status
Never attended school 1 1
Primary education 2.15(1.17–3.79)� 2.16(0.99–4.68)
Secondary education 5.47(2.71–11.05)� 4.93(1.82–13.33)�
�
Higher secondary education and above 6.52(2.89–14.70) 5.92(1.81–19.33)
and above
Occupation
Business 1 1
Service 0.16(0.25–1.03) 0.10(0.00–1.43)
Labor 0.06(0.01–0.26)� 0.22(003–1.45)
�
Home maker 0.09(0.02–0.36) 0.05(0.01–0.322)�
�
Agriculture 0.08(0.02–0.27) 0.12(0.02–0.59)
Monthly income in $ USD
� 100 1 1
>100 2.71(1.70–4.32)� 1.09(0.57–2.10)
No. of child
One 1 1
Two 2.59(1.53–4.40)� 2.65(1.26–5.56)�
Three and above 0.94(0.50–1.77) 2.27(0.92–5.59)
Antenatal care visit
One 1 1
Two 0.71(0.283–1.78) 0.82(0.25–2.70)
)
Three 0.65(0.25–1.72) 0.61(0.17–2.09)
�
Four and above 2.82(1.27–6.25) 2.89(1.04–7.96)�
Postnatal care visit
No visit 1 1
1–2 times 5.49(3.05–9.85)� 1.60(0.68–3.74)
000000000
0
0
0
0
3 times and above 5.24(2.87–9.55)� 2.79(1.16–6.72)�
92.73–14.68)
Place of delivery
(Continued )

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 11 / 18

 Newborn care knowledge and practices in the upper Himalayas

Table 3. (Continued)

Variables UOR (95%CI) AOR (95%CI)

Home 1 1
Health facility 2.23(1.39–3.58)� 0.81(0.36–1.83)
Distance of heath facility (minutes)
<30 4.83(2.39–9.74)� 2.60(0.95–7.07)
�30–59 3.00(1.41–6.39)� 3.66(1.43–9.33)�
�60 1 1
�
P-value <0.05
UOR, Unadjusted Odds Ratio.
AOR, Adjusted Odds Ratio.
1 = Reference.

https://doi.org/10.1371/journal.pone.0222582.t003

another study from Ethiopia showed 80.4% of mothers had good knowledge on essential new-
born care with more than 50% correct answers[24]. The essential newborn care knowledge
demonstrated by mothers in the current study was relatively low and this is perhaps due to dif-
ferences in the study setting as respondents in our study were from the most disadvantaged
geographical location with high illiteracy and limited accessibility to health resources. Further-
more, the quality of the ANC services and its availability at the local health facilities may be sig-
nificant influential factors for the level of mothers’ knowledge, as all the mothers reported at
least one ANC visit.
Mothers’ health related knowledge, attitudes, and behaviour are highly influenced by level
of education [25]. In the present study, adequate newborn care knowledge of mothers was
found to be significantly associated with maternal education, occupation, ANC visit, PNC
visit, household income, age at first pregnancy, and distance of health facility. These results are
comparable to results from studies conducted in Kenya and Ethiopia, where mothers’ educa-
tion, ANC and PNC visits were positively associated with knowledge of newborn care [23,26]
In our study, adequate newborn care knowledge was found to be better among mothers
who had at least primary level education, with increased odds among those with secondary
level education and higher. The mean age at marriage for women in this sample was 17.8 years
and nationally representative surveys also suggest a similar median age at marriage[9].
Approximately 65% of mothers in this study reported that their first pregnancy occurred dur-
ing their adolescent years (15–19 years). This result presents an estimate which is more than
three times higher than the national estimate of adolescent pregnancy[9]. The majority of
mothers in this study visited a health facility for an ANC check-up, however, nearly half of
deliveries took place at home with limited assistance from skilled health workers. This result
was consistent with the Nepal Demographic and Health Survey.
In the current study, the mothers who had attended four or and more ANC visits and three
or more PNC visit were found more than twice more likely to have adequate newborn care
knowledge. This may be due to the dissemination of recommended newborn care related
information adequately disseminated to mothers during ANC and PNC visits[27]. Moreover,
multiple visits perhaps has the potential to increase exposure of mothers to this information,
resulting in improved levels of understanding. Distance to health facilities also plays a crucial
role in utilization of maternal and neonatal services[28,29]. A study conducted at peripheral
health facilities of Nepal revealed mothers who lived less than an hour from a health facility
were more satisfied with maternal health services[28] which is in accordance to the results of
this study. Mothers who lived less than one hour away from a health facility were more likely

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 12 / 18

 Newborn care knowledge and practices in the upper Himalayas

Table 4. Factors associated with adequate maternal newborn care practice among mothers with child less than two years in Dolpa district, Nepal.
Variables Level of practices UOR (95%CI) AOR
Unsatisfactory practices Satisfactory practices n(%) (95%CI)
n(%)
Age of respondent
(Years)
15–20 26(25.5) 30(15.0) 1 1
21–25 36(35.3) 96(48.0) 2.31(1.20–4.42)� 2.65(0.83–8.48)
26–30 34(33.3) 65(32.5) 1.65(0.84–3.23) 1.50(0.40–5.59)
31–35 6(5.9) 9(4.5) 1.30(0.40–4.14) 0.68(0.67–6.96)
Age at marriage
(Years)
� 15 11(10.8) 14(7.0) 1 -
16–20 79(77.5) 168(84.0) 1.67(0.72–3.84) -
21–25 12(11.8) 18(9.0) 1.17(0.40–3.45) -
Age at first pregnancy
(Years)
15–19 76(74.5) 120(60.0) 1 1
20–24 19(18.6) 71(35.5) 2.36(1.32–4.23)� 0.61(0.21–1.73)
25–29 7(6.9) 9(4.5) 0.81(0.29–2.27) 0.72(0.08–5.95)
Ethnicity
Upper caste 51(50.0) 102(51.0) 1 -
Janajati 12(11.8) 26(13.0) 1.08(0.50–2.32) -
Dalit 39(38.2) 72(36.0) 0.92(0.55–1.54) -
Education status
Never attended school 46(45.1) 48(24.0) 1 1
Primary 39(38.2) 64(32.0) 1.57(0.89–2.77) 1.15(0.47–2.81)
Secondary 10(9.8) 52(26.0) 4.98(2.26–10.96)� 1.91(0.54–6.77)
Higher secondary and above 7(6.9) 36(18.0) 4.92(1.99–12.18)� 1.95(0.46–8.15)
Family income (USD)
� 100 79(77.5) 70(35.0) 1 1
>100 23(22.5) 130(65.0) 6.37(3.8–11.03)� 4.17(1.75–9.69)�
No. of child
One 43(42.2) 61(30.5) 1 1
Two 33(32.4) 100(50.0) 12.13(1.22–3.71)� 1.04(0.37–2.90)
Three and more 26(25.5) 39(19.5) 1.05(0.56–1.98) 1.34(0.37–4.78)
Distance to health facility (minutes)
<30 42(41.2) 120(60.0) 3.45(1.81–6.58)� 2.05(0.65–6.42)
30–59 31(30.4) 56(28.0) 2.18(1.08–4.38)� 1.68(0.58–4.85)
� 60 29(28.4) 24(12.0) 1 1
Antenatal care visit
One 15(14.7) 15(7.5) 1 1
Two 26(25.5) 30(15.0) 1.15(0.47–2.80) 1.83(0.46–7.21)
Three 22(21.6) 24(12.0) 1.09(0.43–2.73) 1.69(0.422–6.82)
Four and above 39(38.2) 131(65.5) 3.35(1.50–7.47)� 2.01(0.62–6.51)
Postnatal care visit
No visit 64(62.7) 34(17.0) 1 1
1–2 times 21(20.6) 100(50.0) 8.96(4.78–16.79)� 2.61(0,966–7.06)
3 times and above 17(16.7) 66(330) 7.30(371–14.37)� 3.27(1.16–9.20)�
Place of delivery
(Continued )

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 13 / 18

 Newborn care knowledge and practices in the upper Himalayas

Table 4. (Continued)

Variables Level of practices UOR (95%CI) AOR

Unsatisfactory practices Satisfactory practices n(%) (95%CI)
n(%)
Home 97(95.1) 83(41.5) 1 1
�
Health facility 5(4.9) 117(58.5) 27.34(10.66–70.13) 18.73(5.53–63.46)
Knowledge of newborn care
Inadequate 85(83.3) 62(31.0) 1 1
Adequate 17(16.7) 138(69.0) 11.12(6.10–20.29)� 15.35(5.82–40.47)�
�
P-value <0.05
UOR, Unadjusted Odds Ratio.
AOR, Adjusted Odds Ratio.
1 = Reference.

https://doi.org/10.1371/journal.pone.0222582.t004

to have adequate knowledge and report satisfactory practices in this current study. Therefore,
the assumption may be made that mothers living nearer to a health facility (< 1 hour walking
distance) may be more likely to access healthcare for minor illness during pregnancy, as well
as the postnatal period. Providing mothers with opportunities to easily access health personnel,
improves overall health and knowledge regarding neonatal care.
The study revealed an overall satisfactory level of newborn care practice, which was 66.2%.
However, less than half of total mothers had practiced delayed bathing of the newborn (after
24 hours of birth), skin to skin contact, immediate wrapping of newborn with clean and soft
cloths, and at least three PNC check-ups. Furthermore, results from NDHS 2016 showed only
one in five newborns (21%) received postnatal check-ups within the first hour of life, 75% of
newborns received a postnatal check within the first 2 days following birth and the proportion
who received three complete postnatal checks was very low[9]. With the aim of preventing
neonatal health complications, the Government of Nepal recommends at least three postnatal
checkups for newborns within 7 days of delivery, however results from both the present study
and findings from NDHS 2016 suggest that further improvements need to be made in order to
increase the number of postnatal check-ups that newborns receive. [9].
The Government of Nepal has promoted institutional delivery to prevent neonatal mortal-
ity and morbidity[30]. In our study, only 40.4% of last deliveries were conducted in a health
facility. Many studies conducted in Nepal justified that the low institutional delivery rate was
due to transportation inaccessibility, high additional indirect costs associated with institutional
delivery and the longstanding tradition of home delivery [31,32]. In addition, 14.0% of new-
borns had their cord cut with a Sickle (Hasiya) and used non sterile blade. These traditional
methods of cord cutting and caring practices have been reported as major risk factors for cord
infection and neonatal tetanus [33,34]. Likewise, 62.2% had practiced safe cord caring practice
(used chlorexidine in cord stump), whereas nearly 26% of mothers had applied butter/cooking
oil/mustard oil/turmeric powder on the cord stump and 10.6% had applied nothing to the
cord stump. In order to prevent the development of omphalitis which is a significant risk fac-
tor for neonatal mortality, it is vital that timely cord cleansing occurs with an appropriate anti-
septic solution such as chlorhexidine[35]. Furthermore, prevention of infection at the site of
the umbilical cord is particularly important in contexts such as rural Nepal where the symp-
toms of infection may develop unrecognised[35]. Approximately 67.2% of newborn were
breastfed within an hour of birth. These results are comparable with the NDHS 2016 and simi-
lar studies conducted in rural Nepal [9,12,36]. Several studies in Nepal showed the reasons for

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 14 / 18

 Newborn care knowledge and practices in the upper Himalayas

poor cord cutting and caring practice were mainly due to deep rooted cultural belief, low
awareness regarding cord caring antibiotic ointment (Chlorexidine gel), low maternal educa-
tion, inability to perceive risks of poor neonatal care practices and less awareness about
hygienic delivery kit or difficulty in obtaining the kit locally [12,36,37].
Furthermore, good newborn care practices were more than three folds higher among moth-
ers who had a household monthly income over $100 USD and those who have completed at
least three postnatal visits. Supporting this finding, studies conducted in Ethiopia and Pakistan
also reported association between good newborn care practices and mothers’ newborn care
knowledge and level of wealth [23,38,39]. In contrast, a study conducted by Misgna et al.
showed no association between newborn care practices and income status [24]. The differ-
ences are perhaps due to differences in modality of the health care delivery system, availability
of health services and awareness strategies for maternal and child health in different countries
[24]. Likewise, the probability of good practice was more than fifteen times higher among
mothers who had adequate newborn care knowledge. The result is comparable with a study
conducted in Ethiopia in which mothers who had adequate newborn care knowledge were
more likely to demonstrate good practice [24].
While this study advances our knowledge in the area of newborn care knowledge and prac-
tices, it is not without limitations. First, the results regarding the practice of newborn care are
based on mothers’ recall. To minimize the recall bias, the interview was only limited to moth-
ers with children under two years. Since the study was of a cross-sectional design, predication
of strong causal relationships was not possible. Some of the results with wide confidence inter-
vals due to the small sample size, may limit the precise estimates of the strength of association.
Similarly, it was not feasible to observe the newborn care practices and so, the information was
solicited from mothers through use of questionnaires, which may lead to social desirability
bias. Despite these limitations, the study has explored the maternal newborn care knowledge
and newborn care practices in the upper Himalayas that has been not attempted by earlier
studies. The findings of this study can play a crucial role in planning interventions, particularly
for districts in the mountainous region of Nepal and similar settings. Additionally, this study
has also set the background for further qualitative studies to further understanding and explore
the possibilities of discouraging traditional beliefs and cultural practices of newborn care that
are associated with high risks of neonatal morbidity and mortality in this region. Likewise, it
can be suggested that there should be further exploration of the linkages between newborn
outcomes and knowledge and practices within contexts of poverty, gender disparity and vast
geographical variation. In line with the 2016 United Nations Sustainable Development Agenda
of “leaving no one behind”, this study focuses on a sub-population in a hard-to-reach district
where there is limited data availability to ensure that those who are most disadvantaged are
also represented[40].

Conclusion
The study revealed a high prevalence of inadequate newborn care knowledge and almost one
third of mothers practiced poor newborn care in upper Himalayan dwellings. The results of
our study suggest that increased frequency of ANC and PNC visits likely enhances the knowl-
edge and practices of newborn care. Promotion of institutional delivery and postnatal visits
both for both mothers and newborns needs to be encouraged through the telemonitoring sys-
tem as well as regular follow-up visits at home for neonatal care, in additional to the current
safe motherhood scheme. Awareness of safe cord cutting and caring practices needs to be dis-
seminated. Since a significant number of mothers had reported long walking distances to
reach a health facility as a major hindering factor in maternal and neonatal service utilization,

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 15 / 18

 Newborn care knowledge and practices in the upper Himalayas

the local government should invest in universal health coverage to increase the accessibility of
health services for mothers and newborns.

Supporting information
S1 File. Raw data set.
(SAV)
S2 File. English version of tools.
(DOCX)
S3 File. Nepali version of tools.
(DOCX)

Author Contributions
Conceptualization: Devendra Raj Singh, Kshitij Karki.
Data curation: Pushpalata Bohara, Dhirendra Nath, Sunita Singh, Sylvia Szabo, Kshitij Karki.
Formal analysis: Devendra Raj Singh, Sylvia Szabo, Kshitij Karki.
Investigation: Devendra Raj Singh, Dhirendra Nath, Sunita Singh, Sylvia Szabo, Kshitij Karki.
Methodology: Devendra Raj Singh, Sunita Singh, Kshitij Karki.
Project administration: Devendra Raj Singh, Sunita Singh.
Resources: Pushpalata Bohara, Dhirendra Nath, Sunita Singh, Sylvia Szabo.
Supervision: Devendra Raj Singh.
Validation: Devendra Raj Singh.
Writing – original draft: Devendra Raj Singh, Kshitij Karki.
Writing – review & editing: Devendra Raj Singh, Chloe M. Harvey, Pushpalata Bohara, Dhir-
endra Nath, Sunita Singh, Sylvia Szabo, Kshitij Karki.

References
1. World Health Organization. World Health Statistics 2018: monitoring health for the SDGs, sustainable
development goals [Internet]. Geneva; 2018. Available: http://apps.who.int/iris/bitstream/handle/10665/
272596/9789241565585-eng.pdf?ua=1
2. Liu L, Oza S, Hogan D, Perin J, Rudan I, Lawn JE, et al. Global, regional, and national causes of child
mortality in 2000–13, with projections to inform post-2015 priorities: An updated systematic analysis.
Lancet. 2015; https://doi.org/10.1016/S0140-6736(14)61698-6
3. United Nations Children’s Fund (UNICEF). The State of the World’s Children 2016: A fair chance for
every child [Internet]. New York, NY, USA; 2016. Available: www.soapbox.co.uk
4. Lawn JE, Cousens S, Zupan J. 4 million neonatal deaths: When? Where? Why? Lancet. 2005; 365:
891–900. https://doi.org/10.1016/S0140-6736(05)71048-5 PMID: 15752534
5. Neonatal and Perinatal Mortality Country, Regional and Global Estimates World Health Organization
[Internet]. 2006. Available: http://apps.who.int/iris/bitstream/handle/10665/43444/9241563206_eng.
pdf?sequence=1&isAllowed=y
6. World Health Organization. WHO Recommendation on Newborn Health: Guidelines Approved by the
WHO Guidelines Review Committee [Internet]. Geneva; 2017. Available: http://apps.who.int/iris/
bitstream/handle/10665/259269/WHO-MCA-17.07-eng.pdf?sequence=1
7. National Planning Commission. Nepal and the Millennium Development Goals Final Status Report
2000–2015 [Internet]. Kathmandu, Nepal; 2016. Available: https://www.npc.gov.np/images/category/
MDG-Status-Report-2016_.pdf

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 16 / 18

 Newborn care knowledge and practices in the upper Himalayas

8. United Nations Children’s Fund;, National Planning Commission. Monitoring the situation of children
and women Nepal: Multiple Indicator Cluster Survey Final Report 2014 [Internet]. Kathmandu, Nepal;
2015. Available: http://unicef.org.np/uploads/files/597341286609672028-final-report-nmics-2014-
english.pdf
9. Ministry of Health; New ERA; and ICF. Nepal Demographic and Health Survey 2016. Kathmandu,
Nepal; 2017.
10. Department of Health Services. Annual Report: Department of Health Services (2015/2016) [Internet].
Kathmandu, Nepal; 2017. Available: http://dohs.gov.np/wp-content/uploads/2017/06/DoHS_Annual_
Report_2072_73.pdf
11. Ministry of Health and Population. Nepal’s Every Newborn Action Plan [Internet]. Kathmandu, Nepal;
2016. Available: https://www.healthynewbornnetwork.org/hnn-content/uploads/NENAP-final-low-
resolution.pdf
12. Paudel D, Thapa Anil;, Shedain PR, Paudel Bhuwan. Trends and Determinants of Neonatal Mortality in
Nepal:Further Analysis of the Nepal Demographic and Health Surveys, 2001–2011 [Internet]. Kath-
mandu, Nepal; 2011. Available: https://dhsprogram.com/pubs/pdf/FA75/FA75.pdf
13. Neupane S, Doku DT. Neonatal mortality in Nepal: A multilevel analysis of a nationally representative. J
Epidemiol Glob Health. 2014; 4: 213–222. https://doi.org/10.1016/j.jegh.2014.02.001 PMID: 25107657
14. Lamichhane R, Zhao Y, Paudel S, Adewuyi EO. Factors associated with infant mortality in Nepal: a
comparative analysis of Nepal demographic and health surveys (NDHS) 2006 and 2011. https://doi.org/
10.1186/s12889-016-3922-z PMID: 28068969
15. Bhattarai B. Safe Motherhood in the Context of Nepal. Marriage Fam Rev. Taylor & Francis Group;
2008; 44: 318–327. https://doi.org/10.1080/01494920802255943
16. Kaphle S, Hancock H, Newman LA. Childbirth traditions and cultural perceptions of safety in Nepal: Crit-
ical spaces to ensure the survival of mothers and newborns in remote mountain villages. 2013; https://
doi.org/10.1016/j.midw.2013.06.002 PMID: 23845450
17. Ministry of Health and Population; New ERA; and ICF. Nepal Demographic and Health Survey 2011
[Internet]. Kathamndu, Nepal; 2011. https://doi.org/10.1007/978-94-6091-391-4_15
18. Central Bureau of Statistics. National Population and Housing Census 2011 [Internet]. Kathmandu,
Nepal; 2014. Available: http://umeshg.com.np/nepal/
19. Glenton C, Scheel IB, Pradhan S, Lewin S, Hodgins S, Shrestha V. The female community health volun-
teer programme in Nepal: Decision makers’ perceptions of volunteerism, payment and other incentives.
Soc Sci Med. 2010; 70: 1920–1927. https://doi.org/10.1016/j.socscimed.2010.02.034 PMID: 20382464
20. Al-Ayed IH. Mothers’ knowledge of child health matters: Are we doing enough? J Family Community
Med. Wolters Kluwer—Medknow Publications; 2010; 17: 22–8. https://doi.org/10.4103/1319-1683.
68785 PMID: 22022667
21. Bloom BS. Taxonomy of Educational Objectives. New York, USA: David McKay Company Inc; 1956.
22. Abdullahi A, Hassan A, Kadarman N, Saleh A, Baraya YS, Lua PL. Food safety knowledge, attitude,
and practice toward compliance with abattoir laws among the abattoir workers in Malaysia. Int J Gen
Med. Dove Press; 2016; 9: 79–87. https://doi.org/10.2147/IJGM.S98436 PMID: 27110137
23. Berhea TA, Belachew AB, Abreha GF. Knowledge and practice of Essential Newborn Care among
postnatal mothers in Mekelle City, North Ethiopia: A population-based survey. Biemba G, editor. PLoS
One. Public Library of Science; 2018; 13: e0202542. https://doi.org/10.1371/journal.pone.0202542
PMID: 30133525
24. Misgna HG, Gebru HB, Birhanu MM. Knowledge, practice and associated factors of essential newborn
care at home among mothers in Gulomekada District, Eastern Tigray, Ethiopia, 2014. BMC Pregnancy
Childbirth. BioMed Central; 2016; 16: 144. https://doi.org/10.1186/s12884-016-0931-y PMID: 27329372
25. Paudel D. Trends and Determinants of Neonatal Mortality in Nepal [FA75]. 2011.
26. Gathoni Kibaru E, Otara AM. Knowledge of neonatal danger signs among mothers attending well baby
clinic in Nakuru Central District, Kenya: cross sectional descriptive study. BMC Res Notes. 2016; 9:
481. https://doi.org/10.1186/s13104-016-2272-3 PMID: 27782863
27. Dhakal S, Chapman GN, Simkhada PP, van Teijlingen ER, Stephens J, Raja AE. Utilisation of postnatal
care among rural women in Nepal. BMC Pregnancy Childbirth. 2007; 7: 1–9. https://doi.org/10.1186/
1471-2393-7-1
28. Mehata S, Paudel YR, Dariang M, Aryal KK, Paudel S, Mehta R, et al. Factors determining satisfaction
among facility-based maternity clients in Nepal. BMC Pregnancy Childbirth. BMC Pregnancy and Child-
birth; 2017; 17: 1–10. https://doi.org/10.1186/s12884-016-1183-6
29. Acharya LB, Cleland J. Maternal and child health services in rural Nepal: does access or quality matter
more? Health Policy Plan. 2000; 15: 223–9. https://doi.org/10.1093/heapol/15.2.223 PMID: 10837046

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 17 / 18

 Newborn care knowledge and practices in the upper Himalayas

30. Family Health Division. Aama Surakshya Programme Policy Guideline [Internet]. Kathmandu, Nepal;
2009. Available: http://www.mohp.gov.np/app/webroot/upload/files/Aama_Programme_A_Brief_
Introduction.pdf
31. Sharma SR, Poudyal AK, Devkota BM, Singh S. Factors associated with place of delivery in rural
Nepal. BMC Public Health. BMC Public Health; 2014; 14: 1–7. https://doi.org/10.1186/1471-2458-14-1
32. Shah R, Rehfuess EA, Maskey MK, Fischer R, Bhandari PB, Delius M. Factors affecting institutional
delivery in rural Chitwan district of Nepal: A community-based cross-sectional study. BMC Pregnancy
Childbirth. 2015; 15: 1–14. https://doi.org/10.1186/s12884-015-0429-z
33. Bennett J, Ma C, Traverso H, Agha SB, Boring J. Neonatal tetanus associated with topical umbilical
ghee: covert role of cow dung. Int J Epidemiol. 1999; 28: 1172–1175. https://doi.org/10.1093/ije/28.6.
1172 PMID: 10661665
34. Mullany LC, Darmstadt GL, Khatry SK, Tielsch JM. Traditional massage of newborns in Nepal: Implica-
tions for trials of improved practice. J Trop Pediatr. 2005; 51: 82–86. https://doi.org/10.1093/tropej/
fmh083 PMID: 15677372
35. Mullany LC, Darmstadt GL, Khatry SK, Katz J, Leclerq SC, Shrestha S, et al. Topical applications of
chlorhexidine to the umbilical cord for prevention of omphalitis and neonatal mortality in southern Nepal:
A community-based, cluster-randomised trial. Lancet. 2006; https://doi.org/10.1016/S0140-6736(06)
68381-5 PMID: 16546539
36. Sreeramareddy CT, Joshi HS, Sreekumaran B V., Giri S, Chuni N. Home delivery and newborn care
practices among urban women in western Nepal: A questionnaire survey. BMC Pregnancy Childbirth.
2006; 6: 1–10. https://doi.org/10.1186/1471-2393-6-27 PMID: 16928269
37. Beun MH, Wood SK. Acceptability and use of clean home delivery kits in Nepal: A qualitative study. J
Heal Popul Nutr. 2003; 21: 367–373.
38. Berhe M, Medhaniye AA, Kahsay G, Birhane E, Abay M. Essential neonatal care utilization and associ-
ated factors among mothers in public health facilities of Aksum Town, North Ethiopia, 2016. 2017;
https://doi.org/10.1371/journal.pone.0175902 PMID: 28423043
39. Gul S, Khalil R, Yousafzai MT, Shoukat F. Newborn care knowledge and practices among mothers
attending pediatric outpatient clinic of a hospital in Karachi, Pakistan. Int J Health Sci (Qassim). Qassim
University; 2014; 8: 167–75. Available: http://www.ncbi.nlm.nih.gov/pubmed/25246884
40. United Nations. The Sustainable Development Goals Report [Internet]. New York; 2016. Available:
https://unstats.un.org/sdgs/report/2016/TheSustainableDevelopmentGoalsReport2016.pdf

PLOS ONE | https://doi.org/10.1371/journal.pone.0222582 September 16, 2019 18 / 18