Assignment 5: Volatile Oils: Volatile Oil Fixed Oil

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ASSIGNMENT 5: VOLATILE

OILS
1.By using a table, compare volatile oils with fixed oils. 

VOLATILE OIL FIXED OIL


Also called as an essential oil. Also called as natural non-volatile oil.
Volatile oil can evaporate when placed Fixed oils do not evaporate at room
under room temperature temperature
They can be extracted easily by the They require some specific techniques for
distillation process extraction.

There is no spot (no permanent stain) left Some type of spot (permanent stain) left
after evaporation after evaporation
They are unable to undergo saponification Fixed oils can be easily sponified.
Mixtures of cleoptenes & stearoptenes are Esters of higher fatty acids & glycerin are
termed as volatile oils called as fixed oils.

Posses high refractive index Posses low refractive index


These are optically active. These are optically inactive.

Their primary source is leaves,roots,in Their major source is seeds of the plant.
petals and bark.

2. By using a diagram, show the biosynthetic pathway in the formation of volatile


oils. Explain the process. 

FATB KASII Acety-Coa

FAS ACCase

FATB
Mal-Coa

FATA SAD

Mal- ACP

Acyl- Coa ER
Pool

B. FFA

LACS PC POOL ER

LPCAT

18:1- COA 18:1-PC

FAD2/ FAD3

LPC

PUFA-CoA PUFA-PC

Fatty acid (FA) synthesis is localized to plastids (Figure 1a), whereas assembly of the
TAG molecule occurs outside the plastid and may be associated with both the
endoplasmic reticulum (ER) and the oil body. In most seeds, carbon is delivered to FA
synthesis via glycolysis with hexose and/or triose as the predominant carbohydrate
entering the plastid. However, green seeds can also use light to supply NADPH and
ATP, which allows a ‘bypass’ of glycolysis via ribulose-1,5-bisphosphate carboxylase
activity and pentose phosphate enzymes. This alternative pathway is more carbon
efficient, resulting in 20% more acetyl-CoA available for oil synthesis, and also does not
require reductant supply from the oxidative pentose phosphate pathway. The plastid FA
synthesis pathway determines the chain length (up to 18 carbons) and the level of
saturated FAs in seed oils. The first committed enzyme in the pathway is acetyl-CoA
carboxylase (ACCase). As in yeast, animals and bacteria, plant ACCase is highly
regulated and is a key control point over the flux of carbon into FAs. In addition to
control by phosphorylation, redox status and PII interactions, feedback on ACCase by
18:1-ACP has recently been described. Assembly of FAs occurs on acyl carrier protein
(ACP) via a cycle of 4 reactions that elongate the acyl chain by 2 carbons each cycle.
After 7 cycles, the saturated 16 carbon acyl-ACP can either be hydrolyzed by the FATB
acyl-ACP thioesterase or further elongated by KASII to 18:0-ACP, which is then
desaturated to 18:1-ACP and hydrolyzed by the FATA thioesterase. The resulting 16:0
and 18:1 free acids are the main products of plastid FA synthesis, and their relative
proportions are determined by the activities of FATA, FATB, 18:0-ACP desaturase
(SAD) and KASII. Transgenics and mutants have demonstrated that seed FA chain
length and saturation can be altered by manipulation of any of these four enzymes.

3. Define the following: 

a. Concrete - structural material consisting of a hard, chemically inert particulate


substance, known as aggregate (usually sand and gravel), that is bonded together by
cement and water.

b. Absolute - not mixed or adulterated; pure, something that is not dependent upon
external conditions for existence or for its specific nature, size

c. Resinoid - any of a class of resinous preparations made by pouring a concentrated


alcoholic extract of a drug into cold water and separating and drying the precipitate
formed

d. Pomade - An ointment or cream containing medicaments; usually used on the hair.

References: Hayat, D. (2013, April 15). What is the difference between volatile oil and fixed oil?
Retrieved March 11, 2021, from https://medimoon.com/2013/04/what-is-the-difference-
between-volatile-oil-and-fixed-oil/?
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Afb46ieIPoLRXbvleTq2FCxrYHmO0-jQ-
wBv3kHyi3oh8460SrnkjrB_8pHJFR5rGTdAHLG_9jqPKvtmT3Ov_WKyX_7mykXkJYD
v-H21lFg-Jwo9Y9-mHolcw3RfKIgGotY5FnaYlYRSRzHN2jnKFWCmO-
VZqIaokGIbuVkIY5eY3lsCaTsf8u9wcqWzaEYh_6WylnwL7Uuj6ilXhQCQpVFTLgP93
LBCgNDtLdR6k8veGcmtAnpmrpQEu3t9B5V2gkEdTBVGUzcWpC3uuQ-
PyagCoC8WjvWwcKXQNulLzCBXsXxyqiDceRHuZXb3Lm4wLr607Znv1SxBFNIilKv
OtX9ZcndJXD3S17oaOnx8yyqMIbnudd4ZXJYVZYP_msr3u7heuEHgINPWb_te2dqSH
WQBRIYiy3JLMEbgLiNioPw4
Resinoid. (n.d.). Retrieved March 11, 2021, from
https://www.merriam-webster.com/dictionary/resinoid#:~:text=Medical%20Definition
%20of%20resinoid,Comments%20on%20resinoid

Pomade. (n.d.). Retrieved March 11, 2021, from https://medical-


dictionary.thefreedictionary.com/pomade

Concrete. (n.d.). Retrieved March 11, 2021, from


https://www.britannica.com/technology/concrete-building-material

Bates, P., Stymne, S., & Ohlrogge, J. (2013, March 23). Biochemical pathways in seed Oil
synthesis. Retrieved March 11, 2021, from
https://www.sciencedirect.com/science/article/pii/S1369526613000356

Absolute. (n.d.). Retrieved March 11, 2021, from https://www.dictionary.com/browse/absolute

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