28/07/2020 Introduction to Mechanobiology - - Unit 4 - Week 3

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Unit 4 - Week 3

Course
outline Week3_Assignment3
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Week 1 1) To understand the unfolding signatures of a protein X, a researcher decided to engineer this 1 point
protein in combination with protein Y, whose unfolding signature is already known. Which of the following
Week 2 combination(s) should be used for obtaining better result?
'n’ is the number of residues
Week 3
(X)n-(Y)n
Lecture 11 : (X-Y)n
Protein Both a and b are equally efficient
Unfolding using
AFM (unit?
None of the above
unit=23&lesson=24) No, the answer is incorrect.
Score: 0
Lecture 12 :
Accepted Answers:
Protein
(X-Y)n
Unfolding using
AFM (unit? 2) What is the maximum number of peaks that can be obtained in an unfolding experiment of a 1 point
unit=23&lesson=25) protein with some unstructured domains and 3 folded domains using AFM?
Lecture 13 :
Focal
0
Adhesions: 3
Focal Adhesion
4
Proteins (unit?
unit=23&lesson=26) 5

Lecture 14 :
No, the answer is incorrect.
Score: 0
Focal Adhesion
Accepted Answers:
Organization
5
(unit?
unit=23&lesson=27) 3) Which of the following structures in adherent cells is also known as “Dynamic fixtures” 1 point

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28/07/2020 Introduction to Mechanobiology - - Unit 4 - Week 3

Lecture 15 : Cytoskeleton
Focal
ECM
Adhesions: Role
of Forces (unit? Focal adhesions
unit=23&lesson=28) Plasma membrane
Download No, the answer is incorrect.
Videos (unit? Score: 0
unit=23&lesson=29) Accepted Answers:
Focal adhesions
Quiz :
Week3_Assignment3 4) Fibroblasts when cultured on a soft matrix have been observed to have rounded morphology 1 point
(assessment? while when cultured on a stiff matrix it has spread morphology. What would be the number of focal
name=85) adhesions observed in both cases?
Assignment 3
Higher on the soft matrix and lower on the stiff matrix
solution (unit?
unit=23&lesson=92) High on both the matrices

Weekly
Lower on the soft matrix and higher on the stiff matrix
Feedback (unit? Low in both the matrices
unit=23&lesson=30)
No, the answer is incorrect.
Score: 0
Week 4
Accepted Answers:
Lower on the soft matrix and higher on the stiff matrix
Week 5
5) RGD can prevent blood clotting because 1 point
Week 6
Affinity of RGD is much higher towards α2β3 integrin than to fibrinogen

Week 7 Affinity of α2β3 integrin is higher towards fibrinogen than RGD

Affinity of RGD is much lower towards α2β3 integrin than to fibrinogen
Week 8 Affinity of RGD is much higher towards collagen than to fibrinogen
No, the answer is incorrect.
Text Transcripts Score: 0
Accepted Answers:
Affinity of RGD is much higher towards α2β3 integrin than to fibrinogen

6) Which type of microscopy is suitable for studying the 3D position of proteins? 1 point

Atomic force microscopy

Epifluorescence microscopy
Phase contrast microscopy
Super resolution microscopy
No, the answer is incorrect.
Score: 0
Accepted Answers:
Super resolution microscopy

7) _________ are present at the periphery of an adherent cell, while _________ at its centre. 1 point

Fibrillar adhesions, Focal adhesions

Invadopodia, Focal adhesions
Focal adhesions, Fibrillar adhesions
Focal adhesions, Invadopodia
No, the answer is incorrect.
Score: 0
Accepted Answers:
Focal adhesions, Fibrillar adhesions

8) Find the correct order of maturation of focal adhesions 1 point

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28/07/2020 Introduction to Mechanobiology - - Unit 4 - Week 3

Protrusion --> focal adhesion --> fibrillar adhesion --> focal complexes
Protrusion --> focal complexes --> focal adhesion --> fibrillar adhesions
Protrusion --> fibrillar adhesion --> focal complexes --> focal adhesion
Protrusion --> focal complexes --> fibrillar adhesions --> focal complexes
No, the answer is incorrect.
Score: 0
Accepted Answers:
Protrusion --> focal complexes --> focal adhesion --> fibrillar adhesions

9) Statement A: Larger focal adhesions at cell periphery corresponds to higher forces. 1 point
Statement B: Focal adhesion size as well as magnitude of force decreases near the nucleus.

Only statement A is correct

Only statement B is correct
Both statements are correct
Both statements are incorrect
No, the answer is incorrect.
Score: 0
Accepted Answers:
Both statements are correct

10)State True or False: “Force drives maturation of focal adhesions and force is required to 1 point
stabilize focal adhesions.”

True
False
No, the answer is incorrect.
Score: 0
Accepted Answers:
True

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