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    Standard Operating Procedure Title: Determination of PH GTP Number Supersedes Standard Effective Date

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    8662
    This standard operating procedure outlines the steps for determining pH using a pH meter and electrode. It describes preparing the electrode by cleaning, shaking, and inspecting it. It also details calibrating the electrode using pH buffers, taking measurements by immersing the electrode in samples, and maintaining the electrode through regular cleaning and storage in solution between uses.

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    Standard Operating Procedure Title: Determination of PH GTP Number Supersedes Standard Effective Date

    Uploaded by

    8662
    33 views2 pages
    This standard operating procedure outlines the steps for determining pH using a pH meter and electrode. It describes preparing the electrode by cleaning, shaking, and inspecting it. It also details calibrating the electrode using pH buffers, taking measurements by immersing the electrode in samples, and maintaining the electrode through regular cleaning and storage in solution between uses.

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    SOP (1)

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    This standard operating procedure outlines the steps for determining pH using a pH meter and electrode. It describes preparing the electrode by cleaning, shaking, and inspecting it. It also details calibrating the electrode using pH buffers, taking measurements by immersing the electrode in samples, and maintaining the electrode through regular cleaning and storage in solution between uses.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    33 views2 pages

    Standard Operating Procedure Title: Determination of PH GTP Number Supersedes Standard Effective Date

    Uploaded by

    8662
    This standard operating procedure outlines the steps for determining pH using a pH meter and electrode. It describes preparing the electrode by cleaning, shaking, and inspecting it. It also details calibrating the electrode using pH buffers, taking measurements by immersing the electrode in samples, and maintaining the electrode through regular cleaning and storage in solution between uses.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    STANDARD OPERATING PROCEDURE

    Title: Determination of pH
    GTP Number SOP-001 Version 00
    Supersedes SOP/001-00 Standard In House
    Effective Date 27/11/2020

    1. Purpose:
    The purpose of this standard operating procedure (SOP) is to establish all the necessary steps to use, store, and care for a pH
    electrode.

    2. Materials:
    • pH meter with electrode
    • Pure water (e.g., deionized (DI), reverse osmosis (RO), distilled)
    • pH calibration buffers (e.g., pH 4.01, 7.01, 10.01)
    • pH electrode cleaning solution
    • pH electrode storage solution
    • Beakers
    • Magnetic stir plate and stir bars (optional)

    3. Setup: (Before You Measure)


    3.1 Electrode Preparation
    • Remove protective cap from the electrode.
    • Inspect the electrode for any scratches or cracks. If present, replace the electrode.
    • Shake the electrode down to remove any air bubbles inside the glass bulb.
    • Ensure that the electrode was cleaned and stored properly (see 5.0 Maintenance).
    • Rinse electrode with pure water to remove any salt deposits.
    3.2 Calibration:
    • Fill a beaker with enough pH calibration buffer
    • Place the electrode in the beaker containing pH calibration buffer and gently stir.
    • Confirm the calibration point when the reading is stable, or when the digits do not change for at least 5 seconds.
    • Repeat for additional calibration points. Be sure to rinse with pure water between calibration points. At least two calibration
    points are recommended.
    NOTE: In general, pH 4 and 7 buffers can last 4-8 weeks after opening, and an alkaline pH buffer (i.e., pH 10) will last 1-2
    weeks after opening.

    4. Measurement:
    • Rinse the electrode with pure water.
    • Immerse the tip in the sample and stir gently or use a magnetic stirrer.

    Prepared By Reviewed By Approved By

    Date Date Date


    STANDARD OPERATING PROCEDURE
    Title: Determination of pH
    GTP Number SOP-001 Version 00
    Supersedes SOP/001-00 Standard In House
    Effective Date 27/11/2020

    • Wait until the reading is stable, or when the digits do not change for at least 5 seconds before recording the measurement.
    • Rinse the electrode with pure water until all residues are removed.
    • Repeat this procedure for additional samples

    5.0 Maintenance: (After You Measure)


    5.1 Electrode Cleaning
    • Fill a 100 mL beaker with approximately 75 mL cleaning solution.
    • Place the pH electrode into the cleaning solution for at least 15 minutes.
    • If a refillable electrode is visibly contaminated, drain the reference electrolyte chamber with a syringe or capillary pipette
    and refill with fresh electrolyte. Allow the electrode to stand upright for one hour.
    • Place in storage solution for at least 1 hour and re-calibrate before next use.
    5.2 Electrode Storage
    • Replace the storage solution in the protective cap or beaker.
    • Submerge the glass bulb and junction in protective cap or beaker with solution.
    • A dry electrode should soak in storage solution for at least one hour prior to use and should be re-calibrated. However,
    overnight is optimal.

    Prepared By Reviewed By Approved By

    Date Date Date

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