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    Investigating Plant Growth Regulators: Student Sheet 6

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    Pedrosa Nard
    This document provides instructions for an experiment investigating the effects of plant growth regulators on Coleus stems. Students are instructed to cut Coleus stem sections containing a node into pairs of "seminodes" to use as controls and tests. For Investigation 1, students remove the leaves from one set of seminodes and place them cut-side down to observe growth. The other set is used as a control. Investigation 2 repeats the process and treats one set with lanolin and the other with lanolin containing the plant growth regulator IAA to observe differences in growth. Students are to examine the samples daily for 4-8 days and record any observations.

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    Investigating Plant Growth Regulators: Student Sheet 6

    Uploaded by

    Pedrosa Nard
    198 views1 page
    This document provides instructions for an experiment investigating the effects of plant growth regulators on Coleus stems. Students are instructed to cut Coleus stem sections containing a node into pairs of "seminodes" to use as controls and tests. For Investigation 1, students remove the leaves from one set of seminodes and place them cut-side down to observe growth. The other set is used as a control. Investigation 2 repeats the process and treats one set with lanolin and the other with lanolin containing the plant growth regulator IAA to observe differences in growth. Students are to examine the samples daily for 4-8 days and record any observations.

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    SAPS-Sheet-6-Investigating-plant-growth-regulators

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    This document provides instructions for an experiment investigating the effects of plant growth regulators on Coleus stems. Students are instructed to cut Coleus stem sections containing a node into pairs of "seminodes" to use as controls and tests. For Investigation 1, students remove the leaves from one set of seminodes and place them cut-side down to observe growth. The other set is used as a control. Investigation 2 repeats the process and treats one set with lanolin and the other with lanolin containing the plant growth regulator IAA to observe differences in growth. Students are to examine the samples daily for 4-8 days and record any observations.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    198 views1 page

    Investigating Plant Growth Regulators: Student Sheet 6

    Uploaded by

    Pedrosa Nard
    This document provides instructions for an experiment investigating the effects of plant growth regulators on Coleus stems. Students are instructed to cut Coleus stem sections containing a node into pairs of "seminodes" to use as controls and tests. For Investigation 1, students remove the leaves from one set of seminodes and place them cut-side down to observe growth. The other set is used as a control. Investigation 2 repeats the process and treats one set with lanolin and the other with lanolin containing the plant growth regulator IAA to observe differences in growth. Students are to examine the samples daily for 4-8 days and record any observations.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Student Sheet 6

    www.saps.org.uk

    Investigating Plant Growth Regulators


    Read these instructions carefully before you start.
    Do not eat or taste the plant material.

    Wear safety goggles when handling reagents.

    cut

    1. Cut sections of Coleus stem to 2. Cut the stem in half, vertically, to 3. Investigation 1: On one set of 5
    include a node (the point where a produce two identical 'seminodes'. seminodes, cut off the leaves
    leaf with a bud in the axil occurs where the petiole (stalk) joins the
    and about 1 cm of stem above Repeat with four more stems so lamina (leaf blade) and lay them
    and below it. you have ten 'seminodes'. Each flat, cut surface down, on wet filter
    pair of 'seminodes' gives a control paper in a clear plastic box (which
    and a test specimen. has a tight fitting lid).

    Lights Lights

    lanolin lanolin + 1% IAA

    4. Keep the leaves on the remaining 5 seminodes 5. Investigation 2: Repeat steps 1 and 2 above.
    (controls) and place them, cut surface down, on wet
    paper in the box with the other set. Cut all ten leaves off at the junction of the petiole and
    lamina.
    Put the lid on the box and seal it in a plastic bag. This
    prevents it drying out. Place under a light-bank. To one set of 5, add a blob of lanolin to the cut end of
    the petiole stump. To the other set add a blob of
    Examine daily for 4 - 8 days. lanolin containing 1% IAA (indole acetic acid).

    Place the boxes under the light-bank as before and


    examine daily.

    For study guides, project ideas, and advice on careers and further study in biology, visit www.saps.org.uk/students

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