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    Lab Report Beetroot

    Uploaded by

    Jorge de Felipe Moreira
    This document summarizes an experiment that investigated the effect of temperature on cell membrane permeability using beetroot tissue samples. Membrane permeability was measured at temperatures of 0, 30, 40, 50, and 60 degrees Celsius. The results showed higher permeability at higher temperatures, though some anomalous results at lower temperatures were likely due to experimental errors during those trials. To improve the experiment, the same researchers should measure all temperatures, samples should be properly shaken, cuvettes held correctly, and the same beetroot used. The conclusion is that membrane permeability increases with temperature from lowest at 30 degrees to highest at 60 degrees.

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    Lab Report Beetroot

    Uploaded by

    Jorge de Felipe Moreira
    247 views3 pages
    This document summarizes an experiment that investigated the effect of temperature on cell membrane permeability using beetroot tissue samples. Membrane permeability was measured at temperatures of 0, 30, 40, 50, and 60 degrees Celsius. The results showed higher permeability at higher temperatures, though some anomalous results at lower temperatures were likely due to experimental errors during those trials. To improve the experiment, the same researchers should measure all temperatures, samples should be properly shaken, cuvettes held correctly, and the same beetroot used. The conclusion is that membrane permeability increases with temperature from lowest at 30 degrees to highest at 60 degrees.

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    Lab report Beetroot

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    This document summarizes an experiment that investigated the effect of temperature on cell membrane permeability using beetroot tissue samples. Membrane permeability was measured at temperatures of 0, 30, 40, 50, and 60 degrees Celsius. The results showed higher permeability at higher temperatures, though some anomalous results at lower temperatures were likely due to experimental errors during those trials. To improve the experiment, the same researchers should measure all temperatures, samples should be properly shaken, cuvettes held correctly, and the same beetroot used. The conclusion is that membrane permeability increases with temperature from lowest at 30 degrees to highest at 60 degrees.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    247 views3 pages

    Lab Report Beetroot

    Uploaded by

    Jorge de Felipe Moreira
    This document summarizes an experiment that investigated the effect of temperature on cell membrane permeability using beetroot tissue samples. Membrane permeability was measured at temperatures of 0, 30, 40, 50, and 60 degrees Celsius. The results showed higher permeability at higher temperatures, though some anomalous results at lower temperatures were likely due to experimental errors during those trials. To improve the experiment, the same researchers should measure all temperatures, samples should be properly shaken, cuvettes held correctly, and the same beetroot used. The conclusion is that membrane permeability increases with temperature from lowest at 30 degrees to highest at 60 degrees.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    INVESTIGATING THE EFFECT OF TEMPERATURE ON

    THE PERMEABILITY OF CELL MEMBRANES

    Graph displays the results of the table below

    Temperatur Membrane Avg.


    e permeability 2 3 4 5 membrane
    1 permeability
    0 0.01 0 0.029 0.031 0.032 0.023
    30 0 0 0.022 0 0 0
    40 0.056 0.036 0.056 0.052 0.033 0.46
    50 0.098 0.106 0.039 0.145 0.113 0.1
    60 0.161 0.006 0.572 0.460 0.219 0.515

    Table of results showing temperature in degrees Celsius

    Evaluation:
    Overall, the experiment went well except for some limitations. One of these limitations
    included the different groups involved in obtaining the results for this investigation.
    Each group was assigned to a specific temperature (0, 30, 40, 50 or 60 degrees Celsius) to
    investigate the permeability of cell membranes. Since we are an uncoordinated group of
    people without supervising each other’s work, we were more exposed to human error. It
    seems as if the results we obtained at 0 or 30 degrees Celsius were mostly incorrect due to
    the contradiction that the lower the temperature the lower the membrane permeability;
    and our results show the opposite. One of the possible reasons for this is that when carrying
    out the experiment for 30 degrees Celsius or 0 degrees Celsius, the test tubes containing the
    beetroot tissue were not shook well enough. This means that the Anthocyanin that was
    released in the process of 5 minutes was not dispersed in the solution. Other anomalies
    were found during this investigation, the reason for this is not clarified, but it could be any
    of the following reasons:

    1- Mistaking dimensions of beetroot samples


    2- Grabbing the Cuvettes from the bottom
    3- Forgot to shake test tubes or weren’t shook hard enough
    4- Different times under the water bath treatment

    On the other hand, the experiment was carried out 5 times all of them at the same time.
    That means that in case that one test tube for some reason ended up being contaminated
    there were 4 others to calculate the average of the experiment. Calculating the average of
    the results obtained without including the anomalies which allowed us to exclude the more
    obvious imperfections that were carried out. The temperatures were all maintained
    throughout the entire experiment, and it was calculated before and during the experiment
    through the thermometer inside the water bath and an external thermometer which both
    displayed the same results.

    To improve this experiment, the following measurements should be considered:

    1- Same group of people calculating the results on all different temperatures


    2- Shake the test tubes properly
    3- Hold cuvettes properly
    4- Use the same beetroot

    Conclusion:
    In conclusion, we calculated the effect of temperature on the permeability of cell
    membranes of a beetroot. Since the vacuole of a beetroot cell contains Anthocyanin (a
    purple red pigment) we were able to observe how this pigment diffused into the water
    solution. Anthocyanin is found in the vacuole of a beetroot cell, that means that for us to
    see that pigment being diffused on the water solution, it will have to diffuse through both
    the vacuole membrane, and the cell membrane. To measure the amount of color compound
    made in this solution, we used a colorimeter. Our results concluded that the permeability of
    cell membranes is at its lowest when at 30 degrees and at its highest at 60 degrees Celsius.
    Throughout this experiment some anomalies were noticeable when plotted on the table,
    and all of these anomalies are most likely due to human error.

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