Chromato Calc
Chromato Calc
Chromato Calc
ChromatoCalc
Parameter Kinetika
Pemisahan HPLC GC
Chromatotron
(Centrifugal-TLC) KK TLC
Analisis Kuantitatif
Analisis Regresi
arie_sudarmanto@ugm
Calc
Calc
GC
TLC
isis Regresi
©Arie BS
arie_sudarmanto@ugm.ac.id
Regression Calculator
KROMATOGRAFI : HPLC
BAKU
Unit Konsentrasi baku = mg/L KURVA KALIBRASI
7000000
Parasetamol Kafein
Peak Area
No. Kons. Peak Kons. Peak
6000000 f(x) = 23429.8513398246 x − 20867.9562903448
Baku Area Baku Area
R² = 0.999506540417336
1 52.5 1242433 10.6 640525 5000000
2 105 2412332 21.1 1290383
3 158 3628124 31.7 1990383 4000000
4 210 4952552 42.3 2661071
5 263 6134657 52.8 3308582 3000000 f(x) = 63512.4697347418 x − 35156.4905913162
R² = 0.9998767044095
2000000
1000000
0
0 50 100 150 200 250 300
Konsentrasi Baku
R e s id u a ls
3
2
1
0
-1 5 10 15 20 25 30
-2
Parasetamol Kafein
Standard deviation of the residuals = 1.69% 0.71%
Parasetamol Kafein
Nilai SE Nilai SE
Slope = 23429.85134 300.567403125 63512.4697347 407.19170379
Intersep = -20867.9563 52408.1738981 -35156.490591 14268.673614
R2 = 0.99950654 0.99987670441
S = 49995.30163 13597.6424783
F = 6076.525265 24328.770405
Sig.F = 4.65E-06 5.81E-07
SAMPEL
Preparasi Sampel
No. Peak Berat Vol. FP Kons.Reg. (mg/L) Kadar (mg/g)
Analit 1 Analit 2 (mg) (mL) Analit 1 Analit 2 Analit 1 Analit 2
1 2732006 696048 825.4 50 100 117.494299 11.5127705 711.74157312 69.740553318
2 2790172 706223 824.6 50 100 119.976858 11.6729753 727.48519518 70.779622254
3 2753876 701026 826.1 50 100 118.427724 11.5911489 716.78806142 70.155845848
4 2748063 696175 825.1 50 100 118.179621 11.5147701 716.15332207 69.778027809
RERATA =
SD =
CV =
KROMATOGRAFI GAS
Kondisi Operasi Instrumen:
KOLOM FASE DIAM
Jenis Kolom : Fused Silica Packing Jenis Fase Diam :
Panjang Kolom : m Ukuran partikel : mm
Diameter Kolom : Chemical bonded :
inner outer
FASE GERAK
Gas pembawa : Helium Make up gas : mL/menit
Tekanan gas : kPa Make up flow : mL/menit
Temperatur Detektor : o
C C
o
Temperatur Injektor : o
C C
o
C
o
C
o
FASE GERAK
Tipe Elusi : Isokratik Eluen : Acetonitrile-Water (1:9)
Kecepatan alir : 1 mL/menit
Profil Kromatogram
00,012
00,010
00,008
Konsentrasi
00,006
00,004
00,002
00,000
0 5 10 15 20 25 30 35 40
No.Fraksi
Column I Column J
ANALIT
Neff :
k' :
α :
Rs :
Kons.Rerata RERATA
Kons.SD SD
Kons.CV CV
SAMPEL: Bobot : mg Volume : mL
Kadar Analit dalam sampel: Analit 1: Analit 2: mg/g sampel
80
60
40
20
0
0 1 2 3 4 5 6 7 8 9 10 11 12 13
Column I Column K