Your Guide To Understanding Carbon Dioxide
Your Guide To Understanding Carbon Dioxide
Your Guide To Understanding Carbon Dioxide
UNDERSTANDING
CARBON DIOXIDE
CO2
An analysis of carbon dioxide
across multiple industries
TABLE OF CONTENTS
04 Who is CO2Meter?
08 Why #CO2SafetyMatters
16 Application: Breweries
20 Application: Wineries
21 Application: Agriculture
24 Application: Livestock
37 CO2 Calibration
41 Glossary
CO2Meter.com 5
a note from
Travis Lenander, CEO
This book, Your Guide to Understanding
Carbon Dioxide (CO2), was written to
provide key insights into the various
applications and industries in which our
gas detectors, analyzers, and monitors
play a vital role. It also provides
beneficial information on the science
of carbon dioxide (CO2) as well as its
importance and uses across different
industries and applications.
CO2Meter.com continues to research
and recognize the ever-evolving
needs for gas detection, analyzation,
and monitoring across all industries.
We continue to improve our current
devices and develop new ones to
provide cutting-edge solutions to our
customers. CO2Meter’s continued
product evolution will meet the demand
of customers, worldwide.
We hope you enjoy this informative
guide and would be happy to provide
you with additional education. Please
visit our website www.co2meter.com.
THE SCIENCE OF
CARBON DIOXIDE (CO2)
and other abundant gases
L
Life as we know it, would not exist without Carbon Dioxide
(CO2). This inert gas is essential to human, animal, and plant
life, as well as our biosphere. Carbon Dioxide (CO2) is created
through both natural and man-made processes. Natural CO2
comes from decomposing plants or animals, human and
animal respiration, and geothermic activity. Man-made CO2
is generated from internal combustion engines,
fermentation, and other industrial processes. In most
applications, the concentration of CO2 used is quite
safe. However, in some cases, the gas can reach
Other titles
for Carbon Dioxide,
concentrations that are hazardous to your health.
include carbon
Carbon Dioxide (iv) oxide, carbonic
Formula: CO2 anhydride and
Molecular Mass: 44.01 g/mol carbon oxide.
Boiling Point: -109.2°F (-78.46°C)
Carbon Dioxide (CO2) is a chemical compound composed – National Institute of
of one carbon and two oxygen atoms. It is often referred to Standards and Technology
as CO2 and is the fourth most abundant gas in the Earth’s
atmosphere. Average measurements in outdoor air are
around 400ppm. CO2 only changes properties when placed
under pressure or at low temperatures. For example, CO2 can turn from
a gas into a liquid and from a liquid into a solid.
CO2Meter.com 7
C
Carbon Dioxide, also known reduced the number
as CO2, is the odorless. of incidents that
colorless, and tasteless gas occur globally.
that humans exhale. For
this reason, it is often hard Continuous CO2
to grasp how dangerous monitoring is
this inert gas actually critical for ensuring
is. But, inhaling CO2 at a safe working
elevated levels can be environment where
deadly. Accidents occurring CO2 is stored or
in restaurants, breweries, created. Anyone
agriculture facilities, and who works near
stadiums have created the created or stored
need for carbon dioxide carbon dioxide
regulations. The National Fire should be protected
Protection Association, The by a safety device.
National Board Inspection
Code, and International Why? Because even healthy adults can
Fire Code utilized pass out in seconds when carbon dioxide is
recommendations from the present at higher concentrations. At lower
Occupational Safety Hazard concentrations, a person can also begin to
Administration experience shortness of breath, fatigue,
and the dizziness, or nausea. Preventing these
“Breathing National negative health effects and serious injuries
concentrations Institute of from occurring is the main goal of carbon
of 30% CO2, Occupational dioxide monitoring and analysis.
even with 70% Safety & Health
Oxygen can lead to to establish Safety monitors alert the user that an area
unconsciousness in exposure has an unsafe level of CO2. Additionally,
just 30 seconds” limits and some monitoring systems provide a two-
requirements for part monitor: one part inside the space
– International Journal of carbon dioxide to measure and warn AND a second part
Emergency Medicine monitoring. outside the space to warn you not to enter
These the space if it is hazardous.
regulations have
CO2Meter.com 9
TOP CO2
HAZARD POINTS
Whether your industry produces, stores, analyzes or uses
CO2, you should always be aware of potential leak points in
your system. Understanding these potential carbon dioxide
leak points allows individuals to prioritize their analysis
on a regular schedule, as well as properly identify the best
locations for installing carbon dioxide safety monitors.
OSHA Confined Locations Near Locations with Locations CO2 is Locations CO2
Space the Floor Restricted Given Off From is Collected or
Ventilation Process Discharged
Including those And low-lying areas
around any CO2 (basements or Keg Coolers BIB pumps, Fire suppression
tank or cylinders. underground storage). fermentation tanks, testing.
mash tanks, drains
10 CO2Meter.com
O
Oversight of workplace safety to which gases are harmful. Visit OSHA to
and hazards are overseen learn more about the half short term exposure,
by the Occupational Safety short term exposure, and immediately
Hazard Administration dangerous to life and health limits.
(OSHA). In conjunction
with the National Institute Since 2012, additional resources have begun
of Occupational Safety to identify serious incidents involving CO2
and Health (NIOSH), the overexposure that have resulted in severe
two agencies have worked injuries and even fatalities. In an effort to
together to research and eliminate these incidents from occurring,
develop guidelines detailing several associations have worked to codify
the specifications of inert specific requirements pertaining to CO2
gases like CO2, their safety and monitoring, while referencing the
properties, and the standard OSHA and NIOSH standards.
CO2Meter.com 11
Life Science
In life science applications, CO2 incubators are used to
grow and maintain cell cultures performing tissue or cell
cultivation. CO2 is a critical component in the incubator
to maintain the pH of the cultures and mediums. Typical
incubators run between 7-10% CO2
CO2Meter.com 13
Agriculture
Growing plants indoors often involves the
use of CO2 for growth enhancement (aka
enrichment). Injecting CO2 into a grow
room is one component of making plants
grow faster and fuller. Grow rooms are
typically kept between 800-1,500 ppm at
all times. Additional environments include
greenhouses, grow rooms, cultivation
facilities and mushroom farms.
Upper left: The IAQ Max Indoor CO2 Monitor and Data Logger right: The Tongdy Wifi
Indoor Air Quality Monitor
CARBON
DIOXIDE APPLICATIONS
16 Application Overview CO2Meter.com
BREWERIES
C
Carbon Dioxide in breweries is two separate tanks. Your
both stored for use and created. brewery’s production output is
a good indication of how much
Stored Carbon Dioxide CO2 you will need.
Stored CO2 in breweries serves
many purposes. The primary Large craft breweries can use
use is to carbonate the beer. more than two tons of CO2
Other uses are in the packaging per day when creating beer.
(cans, bottles, and kegs) as well Depending on where the
as in taproom beer dispensing. CO2 is needed, distribution
For these lines are run
uses, the throughout
brewery's the brewery.
CO2 supplier Larger craft Some older
will select a breweries can use breweries
CO2 storage more than two tons who have
tank that is of CO2 per day. grown over
sized to meet time may have
a brewers’ - Joshua Pringle, VP of a "maze" of
needs. The Business Development CO2 lines as
supplier their system
may provide has grown to
one tank for production and meet production requirements.
dispensing, or they may offer New breweries are often
CO2Meter.com Application Overview 17
designed with these components laid out first. This minimizes the
length of tubing and exposure of carbon dioxide lines.
Upper left to right: 1: Draft cooler lines installed by Liquid Logistics. 2: Remote CO2 Storage Safety
3 Alarm installed at Laguanitas. 3: Copper Kettle Brewing CO. using the CO2 Leak Detector
CO2Meter Recommendations:
Soda Systems
Great soda or pop is created by mixing three
key components: carbon dioxide, water
and soda syrup. Precise portions of each
are required to make the perfect soda. The
soda system uses a carbonator to combine
these ingredients in the precise portions.
The carbonator is just one of several key
components of a carbonated beverage system.
The system also includes: CO2 tank or cylinder,
liquid CO2, BIB (bag in box syrup), BIB pumps,
water purification mechanism, clean water,
carbonator, dispense mechanism (fountain or
gun), and all the associated lines and parts.
WINERIES
M
Much like CO2 in breweries, our winery only 249 worldwide wine
partners can both store and create CO2. professionals, certified by the
court of master sommeliers.
Wineries have five key areas of concern for As a true professional, Miliotes
CO2 concentration build-up. These include knows all the places CO2 is
the fermentation areas, storage tanks, pits, used or created in the wine
barrel cellars, and bottling areas. Carbon making process; and also
dioxide levels can accumulate and increase clearly understands that the
quickly in these areas, creating a health risk. installation of CO2Meter’s
RAD-0102-6 provides the
Like beer production, the fermentation entire team a level of safety
process is a key cause of CO2 build-up. preventing CO2 incidents,”
Again, tanks and vessels are highly hazardous states Director of Marketing,
areas. Lock-out/tag-out, as well as confined Morgan Morris.
space entry procedures, should be strictly
adhered to in these areas.
“George
Miliotes, CO2Meter Recommendations:
a Master
Sommelier
and owner
of Wine
Bar George
in Disney
Springs, RAD-0102-6 | CM-7000 | CO2 SAN-11| Personal SAN-13| CO2
Remote CO2 Storage Multi Sensor System 5% CO2 Monitor & Leak Detector
is one of Safety 3 Alarm Data Logger
CO2Meter.com Application Overview 21
AGRICULTURE
T
The use of carbon dioxide CO2 is used for two main reasons: enrichment
in agricultural applications and extraction.
is abundant. The breadth
of detection, monitoring, CO2 Enrichment
and analytical equipment Enrichment is the process of purposely
runs the gamut from growth “enriching” an indoor grow space with carbon
enrichment, exhausting for air dioxide. This process accelerates the growth
quality, storage monitoring, of the plants. This technique is not specific to
and analysis for quality. CO2 the cannabis market either. Peppers, tomatoes,
is widely used in agriculture. leafy greens, and many others benefit from the
enrichment process. Enrichment using CO2
One of the fastest-growing also has no negative effects on the plant or on
markets in the US is cannabis. the consumers who use the plant.
Over half of the United States
has legalized cannabis for Enrichment involves delivering precise
either medical or recreational amounts of CO2 into a room. That room is
use within the last five years. intentionally held between 800-1,500 ppm;
This has created an enormous this range is the “sweet spot” for enrichment.
surge in the production and The plants absorb the CO2 during their
extraction of cannabis. For photosynthetic process and that “enrichment
the production of cannabis, of the plant” induces an accelerated growth
22 Application Overview CO2Meter.com
C
Carbon Dioxide is often measured in For instance, our partners at Jason Yordy
livestock applications (poultry and swine Enterprises utilize the Cozir Ambient 10,000
especially) as an indicator of animal health. ppm CO2 Sensor with R/H and Temp. inside
Much like Indoor Air Quality measurements their TurkeyTrac product which monitors bird
are to humans, these same air quality health in barns.
measurements are critical to understanding
the overall health and welfare of the animals. In addition, carbon dioxide is becoming the
most commonly accepted method for animal
In poultry barns, CO2 can run in parallel euthanasia. The goal everyone shares, from
to the existence of ammonia. Elevated farmers to scientists, is to make euthanasia
ammonia concentrations, above 5-10 ppm, as painless as possible for the animal.
can indicate unclean conditions in the barns. Results from this process have shown it to
These conditions can cause negative health humane and effective, in both livestock and
effects for the birds. poultry applications.
SCIENTIFIC DISCOVERY
and UNIVERSITY RESEARCH
T
Thousands of researchers in the US examine Key Examples
aspects of CO2 and other gases daily. They The National
build on current information or complete University
new research on a variety of topics. Capstone Project
Researchers are working diligently to expand did research in
their knowledge. For instance, some are capnography. The
testing off-gassing in composted material, experiment studied
indoor air quality, and even soil fluxing! the importance
of carbon dioxide
Who is Researching and oxygen
This research takes place at all levels of concentrations
education. We’ve seen experiments from within an F-35
primary, secondary, and university students, Fighter Pilot’s mask.
as well as within the private sector.
The team used our
Multi-national corporations like Battelle and 0-25% UV Flux
Linde are conducting research with their Oxygen Smart
customers on innovative programs. Colleges Sensor and the K30
and universities continue to expand both lab FS Fast Response
and field-based research on air quality, life 10,000ppm CO2 Sensor in their study. By
sciences, and capnography. Even high school using both sensors together, the researchers
science fair projects are being recognized were able to gain key data analysis. This data
for their innovation and results. then confirmed the specific conditions of
hypoxia in the fighter pilots.
MEDICAL
and L I F E
SCIENCE
L
Long ago are the days of certified gases suitable for
big pharma with huge R&D laboratories and incubation
budgets capable of developing clinics. With the use of gas,
the latest over the counter comes the need for proper
or pharmacy medications. gas detection, incubator
Not anymore. Large sensors, and safety solutions.
pharmaceutical companies As this field is always
have given way to smaller changing, scientists will
biopharmaceutical companies. always need to monitor and
Each one developing its control gas concentrations.
own research and
development, tests, and Sensor Research
marketing. And each Researchers from a
one is hoping to bring California University earned
the latest cancer or a government grant which
Alzheimer’s drug to the utilized one of our
market. Hospitals and best-in-class sensing
medical entities have solutions, the SprintIR-
also gotten into the 6S 20% CO2 Sensor.
market, developing their The medical team at
own resources to drive the university furthered
innovation in the lab. No their research with
matter the funding source the CO2 sensor in
or marketing budget one a rare brain imaging
thing holds true for this application. This study was
entire sector, everyone uses dedicated to understanding
precise gases to create, grow, the structure, function, and
study, and bring to market the direct effect of carbon dioxide
latest life-saving drugs. and cerebral blood flow.
CO2Meter Recommendations:
MODIFIED
ATMOSPHERE
PACKAGING (MAP)
H
Have you ever wondered about the moment you open a carbonated
how that bag of lettuce beverage. Right after the bottle or can is filled
on the shelf at the grocery and right before sealing, a drop of CO2 is
store can stay fresh and released on top of the beverage. This liquid
tasty for so long? It’s called quickly turns in to a gas and expands by
Modified Atmosphere approximately 8.75 times its volume. This quick
Packaging. In MAP expansion combined with the molecular weight
applications an inert gas, of CO2, drives the oxygen in the container
usually carbon dioxide out almost instantly, slightly pressurizing the
or nitrogen, drives out container. After a quick seal, the container is
oxygen from the package ready to keep oxygen out and CO2 in. When
before sealing. In doing so, you open the beverage, that spritz you hear,
the inside of the package is the release of the drop of CO2 from under
now has less opportunity pressure. It is also the final moment before
to convert oxygen into that beverage begins to oxidize. This is why a
oxidation on the product. soda that’s been open for a few hours has no
carbonation and tastes funny or flat.
Foods in MAP
Applications for MAP Another example is fruit packaging and
processes in food production shipping. When it comes to packaging,
are the most well-known. studies of produce have found that the
Meat, fruit, vegetables, and ideal balance of gases in a package is 1-5%
carbonated beverages are oxygen, 2-15% carbon dioxide, and 80-95%
the easiest to visualize. Think nitrogen. Some companies developed sealed
CO2Meter.com Application Overview 31
containers for their produce. These bins can processes to seal sterilized devices such as needles
hold specific gas concentrations, prolonging and surgical instruments. Over-the-counter
the life of the produce. medications use MAP processes to ensure the
quality of the product for the consumer.
Bananas are the easiest to understand. Once
a bunch is cut from the tree and cleaned it Customer Use
is then placed in large shipping or storage One of our customers achieved much higher
boxes. The boxes are then placed in a performance on the production line and in
shipping container, sealed, and a precise gas testing, by using the SprintIR-W 100% CO2
concentration is achieved and maintains the Sensor. This sensor provides twenty readings
proper environment, halting further growth of per second and requires very little power.
the banana. Once at its final destination, the
containers get trucked to ripening facilities. Not only is properly measuring your MAP
These facilities place the bananas in a sealed gases important but so is utilizing proper gas
room with specific temperature and humidity detection and monitoring in your facility.
levels for approximately 5 days. During
this time the room is slowly enriched with #GasSafetyMatters
ethylene gas. The ethylene encourages the MAP requires the storage of massive
bananas to grow and ripen. Have you ever amounts of gas. Like other applications,
gotten a banana that won’t ripen on your these stored gases can leak and create
counter and turns grey in color? You have a potentially life-threatening situations.
banana that didn’t get enough ethylene.
Spot Checking
To test the accuracy of MAP equipment,
a probe is inserted into the packaging.
This tests the concentration of oxygen TS-200 | TecPen SAN-13| CO2 SAN-11| Personal
within the package. Some devices use a MAP Oxygen Leak Detector 5% CO2 Monitor &
resealable septum to pierce the packaging Analyzer Data Logger
so as not to spoil the product. In beverage,
the instrument pierces the can or plastic
bottle. However, these containers are often
discarded after testing.
Each state has jurisdiction over the testing CM-1000 GasLab® Pro
of CO2 suppression systems. Some require (100% CO2) Multi-Gas
an annual certification test to demonstrate Sampling Data Logger.
the effectiveness of the system. The NFPA Users should understand
12 lists the testing criteria for these systems. that if they purchase a
In short, a system must emit enough CO2 to single unit they will need
suppress the fire within a set time period. to conduct three dump
tests to complete the NFPA
NFPA 12 requires testing 12 requirements. CO2Meter
a low, medium, and offers a discount when
high points within the purchasing three devices so
space AND that CO2 that three points in the space
concentrations must reach can be measured during a
and be held at certain single dump test.
levels over a given period
of time.
Right: CM-1000 GasLab® Pro
(100% CO2) Multi-Gas Sampling
CO2Meter designed one of the only devices Data Logger
that meet the requirements prescribed in NFPA
12. We recommend utilizing the
36 CO2Meter.com
INSTALLING A CO2
SAFETY MONITOR
T
The installation of fixed safety monitors is relatively simple.
Some manufacturers design devices that require their
installation and installation fee. However, CO2Meter believes
in, and designs devices that can be self-installed.
Above: Left: Remote CO2 Storage Safety 3 Alarm installed at U.S.. Bank Stadium.
Right: Remote CO2 Storage Safety 3 Alarm installed at Rocket Mortgage FieldHouse.
12 in
Multiple Sensors
While many individuals are required to install fixed safety
monitors, several of our customers have begun installing the
CM-7000 CO2 Multi Sensor System. This is due to its many
unique capabilities and functions. This device exceeds all
national, state, and jurisdictional codes around CO2 safety
monitoring. In addition, the fixed-wall mount sensor system
provides customers in restaurants, breweries, agriculture,
and safety facilities the ability to monitor up to twelve
sensors from an 8” touchscreen control panel.
38 CO2Meter.com
CO2 CALIBRATION
D
Devices from CO2Meter come pre-calibrated from our facility.
Calibration should not need to be conducted upon arrival.
FREQUENTLY
ASKED QUESTIONS
What is the most What range of CO2 Measurement is best for
widely used sensing my application?
technique for real-time
measurement of CO2? We get this question quite frequently and it is important
to understand that carbon dioxide (CO2) sensors
Two main sensor types can measure from 0 ppm all the way to 1,000,000
are used to measure ppm. Selecting the sensor or device that will measure
gases – electrochemical accurately for you depends on many factors including:
(EC) and non-dispersive application, expected temperature and humidity,
infrared (NDIR). other gases present, required rate of measurement,
CO2Meter only uses and others. For example: IAQ applications deeply
NDIR sensors for carbon need a sensor that measures below 2,000 ppm, Fire
dioxide measurement Suppression applications need a sensor that can
for two reasons: the measure at 100% CO2, and incubation applications
accuracy of the sensor typically need a sensor that measures in the 7-10%
and its extremely long range. For help in selecting the right sensor for your
life. By utilizing NDIR application please contact our technical sales team.
sensing technologies
CO2 can be measured
by the absorbance of an
infrared light source at a What is the Difference between CO2 vs. CO?
specified wavelength.
One of the most common questions we get asked is
CO2Meter devices “what is the difference between CO2 vs. CO?” Many
incorporate the latest people believe the two gases are the same, but they
in NDIR technology are very different. CO2 is naturally occurring and
from a variety of is formed in processes such as fermentation, the
extremely reliable sensor burning of fossil fuels, and human respiration. CO, on
manufacturers from the other hand, is entirely man-made - often due to
around the world. incomplete combustion of fossil fuels (gasoline, oil,
and coal). For more information click here.
40 CO2Meter.com
Why does it feel like inspectors are all over CO2 monitoring, right now?
The inclusion of CO2 monitoring into the codes mentioned above only occurred in the
last few years as incidents became more prevalent and understood. Inspectors are now
being trained on these code updates and as such, are inspecting.
GLOSSARY
Alarm Set Point - The point set inside the firmware and
software of a device that triggers the device to alarm, notify,
or trigger relays.
Diffusion – Allowing the gas to pass through the sensor on its own.
STEL (Short Term Exposure Limit) - the STEL is the limit and
time at which OSHA allows for a human to be inside a space
deemed hazardous
Your Turn!
Spread the word on Gas Detection, Compliance, and Safety in
your workplace! Use #CO2SafetyMatters for the chance to be
recognized across our platforms!
ADDITIONAL RESOURCES
MBAA Toolbox Carbon Dioxide NFPA Code IFC Standard National Board CO2Meter
Health Hazard Requirements Development Inspection YouTube
Information Guide Process Code Resource
Sheet
CO2Meter.com 45
REFERENCES
Brewers Association. (2020, April). Guidelines for Economical Use of Carbon
Dioxide in the Brewery. Retrieved May 2020, from Brewers Association.
Castro M. Control of breathing. In: Physiology, Berne RM, Levy MN
(eds), 4th edition, Mosby, St. Louis, 1998.
Dong Sun Lee. Carbon dioxide absorbers for food packaging applications,
Department of Food Science and Biotechnology, Kyungnam University,
Volume 57, Part A, June 2016.
Health and Safety Executive. (2000). General Hazards of Carbon
Dioxide. Retrieved May 2020, from Health and Safety Executive.
International Fire Code. (2018). 2018 IFC Update. Retrieved May 21,
2020, from International Code Council.
International Journal of Emergency Medicine. (2017). Carbon dioxide
poisoning: a literature review of an often forgotten cause of intoxication
in the emergency department. Retrieved May 19, 2020, from National
Center for Biotechnology Information.
National Board Inspection Code. (2020). Carbon Dioxide. Retrieved May
18, 2020, from The National Board of Boiler and Pressure Vessel Inspectors.
National Fire Protection Association. (2019). List of NFPA Codes & Standards.
Retrieved May 20, 2020, from National Fire Protection Association.
National Institute for Occupational Safety and Health (NIOSH). (1989).
Carbon Dioxide. Retrieved May 21, 2020, from Centers for Disease
Control and Prevention.
National Library of Medicine. (2019). Compound Summary – Carbon
Dioxide. Retrieved May 20, 2020, from PubChem.
Occupational Safety and Health Administration (1987). Carbon Dioxide
in Workplace Atmospheres. Retrieved May 2020, from OSHA.gov.
OHS Online. (2016, April). Carbon Dioxide Detection and Indoor Air
Quality Control. Retrieved May 2020 from Occupational Health & Safety.
Science daily. (2018). Carbon Dioxide Reference. Retrieved May 2020,
from Science Daily.
Stanford University (2006, March). Can improvement in photosynthesis
increase crop yields? Retrieved May 19, 2020, from Wiley Online Library.
Wikipedia. (2020). Periodic Table. Retrieved May 26, 2020 from
https://en.wikipedia.org/wiki/Periodic_table.
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