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    Experiment Vi - Group 6

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    Kleya Monique Parreño
    The document summarizes an experiment investigating factors that influence enzyme action of polyphenoloxidase. It examines the effect of substrate concentration, enzyme concentration, temperature, and pH on the enzyme's activity. The experiment shows that enzyme activity increases with higher substrate and enzyme concentrations, peaks at 37°C, and works best at a neutral pH of 7. The results are presented in tables and the student is asked questions to analyze the effects of each factor and determine the optimal conditions for polyphenoloxidase activity.

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    Experiment Vi - Group 6

    Uploaded by

    Kleya Monique Parreño
    52 views3 pages
    The document summarizes an experiment investigating factors that influence enzyme action of polyphenoloxidase. It examines the effect of substrate concentration, enzyme concentration, temperature, and pH on the enzyme's activity. The experiment shows that enzyme activity increases with higher substrate and enzyme concentrations, peaks at 37°C, and works best at a neutral pH of 7. The results are presented in tables and the student is asked questions to analyze the effects of each factor and determine the optimal conditions for polyphenoloxidase activity.

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    FACTORS INFLUENCING ENZYME ACTION

    Original Title

    EXPERIMENT VI - GROUP 6

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    The document summarizes an experiment investigating factors that influence enzyme action of polyphenoloxidase. It examines the effect of substrate concentration, enzyme concentration, temperature, and pH on the enzyme's activity. The experiment shows that enzyme activity increases with higher substrate and enzyme concentrations, peaks at 37°C, and works best at a neutral pH of 7. The results are presented in tables and the student is asked questions to analyze the effects of each factor and determine the optimal conditions for polyphenoloxidase activity.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    52 views3 pages

    Experiment Vi - Group 6

    Uploaded by

    Kleya Monique Parreño
    The document summarizes an experiment investigating factors that influence enzyme action of polyphenoloxidase. It examines the effect of substrate concentration, enzyme concentration, temperature, and pH on the enzyme's activity. The experiment shows that enzyme activity increases with higher substrate and enzyme concentrations, peaks at 37°C, and works best at a neutral pH of 7. The results are presented in tables and the student is asked questions to analyze the effects of each factor and determine the optimal conditions for polyphenoloxidase activity.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Name: KLEYA MONIQUE I.

    PARREÑO Date Performed: 11/10/2021


    Section: C - LAB Date Submitted: 17/10/2021

    Experiment No. 6
    FACTORS INFLUENCING ENZYME ACTION

    I. Data
    A. Effect of Substrate Concentration
    Color Code
    tt A (2 vol catechol:4 vol water) +

    tt B (4 vol catechol:2 vol water) ++

    tt C (6 vol catechol) +++

    B. Effect of Enzyme Concentration


    Color Code
    tt A (5 drops enzyme extract) +

    tt B (10 drops enzymes extract) ++

    tt C (15 drops enzyme extract) +++

    Construct a plot in any spreadsheet application showing enzyme activity (y-axis) versus amount of
    enzyme extract (x-axis). For enzyme activity use the following conversion of color code to numerical
    value: + = 1; ++ = 2; and +++ = 3.
    Test Tube Amount of Enzyme Enzyme Activity
    Extract
    A 5 drops 1
    B 10 drops 2
    C 15 drops 3
    C. Temperature and Enzyme Action
    Color Code
    ++
    tt A (0oC)
    +++
    tt B (37oC)
    +
    tt C (70oC)
    Construct a plot in any spreadsheet application showing enzyme activity (y-axis) versus temperature (x-
    axis). For enzyme activity use the following conversion of color code to numerical value: + = 1; ++ = 2;
    and +++ = 3.

    Enzymes Code Value Temperature (°C)

    tt A 2 0
    tt B 3 30
    tt C 1 70

    D. Effect of pH on Enzyme Activity


    Color Code
    -
    tt A (HCl, pH = 1)
    -
    tt B (lactic acid, pH = 5)
    ++
    tt C (distilled water, pH = 7)
    +
    tt D (Na2CO3, pH = 9)
    Construct a plot in any spreadsheet application showing enzyme activity (y-axis) versus pH (x-axis). For enzyme
    activity use the following conversion of color code to numerical value: + = 1; ++ = 2; and +++ = 3.
    Enzyme pH of Solution Enzyme Activity
    tt A (HCl) 1 -
    tt B (lactic Acid) 5 -
    tt C (distilled water) 7 ++
    tt D (Na2CO3) 9 +

    II. Questions
    1. Based on the graph constructed in Part A, give a statement relating enzyme activity to substrate
    concentration.
    As the substrate concentration increases, the enzyme activity also increases. An optimum rate is reached at the enzyme’s optimum substrate

    concentration.

    2. Explain why at 70oC enzyme activity decreased.


    The rate of an enzyme-catalyzed reaction increases as the temperature increases, as it does with many chemical reactions. The rate, however,

    decreases at high temperatures because the enzyme becomes denatured and can no longer function.

    3. On what type of solution – acidic, basic, or neutral – will polyphenoloxidase work best. Explain your
    answer.
    Since polyphenoloxidase is dependent on the pH, it works best on a neutral solution. The ideal pH of a solution should be close to the pH of 7

    because more acidic and basic solution will slow the reaction and will cause denaturation of the polyphenoloxidase enzyme, which greatly affect its

    function.

    CHEM 20 LAB EXPERIMENT NO. 6 FACTORS INFLUENCING ENZYME ACTION 2

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