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    Zivak Organic Acid LC-MS-MS Urine

    Uploaded by

    Yousra Zeidan
    This document provides information about an organic acid analysis kit that can quantitatively detect 73 major organic acids in human urine samples. The kit uses LC-MS/MS to provide results in just 20 minutes with minimal sample preparation. It includes reagents, calibrators, controls, and an analytical column to test urine samples according to established medical procedures.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Zivak Organic Acid LC-MS-MS Urine

    Uploaded by

    Yousra Zeidan
    211 views2 pages
    This document provides information about an organic acid analysis kit that can quantitatively detect 73 major organic acids in human urine samples. The kit uses LC-MS/MS to provide results in just 20 minutes with minimal sample preparation. It includes reagents, calibrators, controls, and an analytical column to test urine samples according to established medical procedures.

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    Zivak organic Acid LC-MS-MS urine

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    This document provides information about an organic acid analysis kit that can quantitatively detect 73 major organic acids in human urine samples. The kit uses LC-MS/MS to provide results in just 20 minutes with minimal sample preparation. It includes reagents, calibrators, controls, and an analytical column to test urine samples according to established medical procedures.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    211 views2 pages

    Zivak Organic Acid LC-MS-MS Urine

    Uploaded by

    Yousra Zeidan
    This document provides information about an organic acid analysis kit that can quantitatively detect 73 major organic acids in human urine samples. The kit uses LC-MS/MS to provide results in just 20 minutes with minimal sample preparation. It includes reagents, calibrators, controls, and an analytical column to test urine samples according to established medical procedures.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    F U T U R E O F A N A LY S I S

    SUMMARY AND EXPLANATION


    Organic acid disorders (organic acidemia) are a group of inherited metabolic conditions. Most of the organic
    acidemias caused by defective autosomal genes for various enzymes are for amino acid metabolism. Most are
    inherited as autosomal recessive diseases. Organic acid related disorders may affect many metabolic pathways
    including amino acid, lipid metabolism, fatty acid oxidation and the Krebs cycle.
    Organic acidemias are usually diagnosed in infancy, characterized by urinary excretion of abnormal amounts or types
    of organic acids. These disorders vary in their prognosis, from manageable to fatal, and usually affect more than one
    organ system, especially the central nervous system.
    Quantitative analysis of the organic acids in urine samples has clinical importance for determining organic acidemias
    and monitoring the organic acid concentrations in human urine.

    Developed for quantitative detection of the 73 major organic acids in human urine samples.
    Gives results in 20 minutes with minimal sample preparation.
    Main methods and procedures that have been selected are based on EN ISO 13485 and 98/79/EC.

    Analytes

    2-3-pyridine carboxylic Adipic Citric Orotic


    2-OH-phenylacetic AKBM Ethylmalonic P-Hydroxyphenyllactic
    2-me-hippuric AKIC Formimino glutamic Phenylacetic
    2-me-citric AKIV Fumaric Phenylglyoxylic
    2-me-glutaric Alpha-OH-glutaric Hexanoyl glycine Picolinic
    2-me-succinic Alpha-OH-butyric Hippuric Pimelic
    3-OH-propanoic Alpha-OH-isovaleric Homovanillic Propyonil Glycine
    3,4-dihydroxy-hidrocinnamic Alpha-Ketobutyric Iso-Citric Pyroglutamic
    3-phenyllactic Alpha-Ketoglutaric Iso-Valeryl Glycine Pyruvic
    3-indoleacetic Benzoic Kynurenic Sebacic Acid
    3-me-hippuric Beta-OH-butyric Lactic Suberic
    3-me-crotonyl glycine Beta-OH-isovaleric Malic Suberyl glycine
    3-me-glutaric Glycolic Acid Malonic Succinic
    3-OH-3-me-glutaric Glyceric Acid Mandelic Succinyl Aceton
    4-OH-benzoic Glucaric Methylmalonic Tartaric
    4-me-hippuric Glutaric Acid Mevalonolactone Tiglyl glycine
    4-OH-phenylacetic Cis-aconitic N-(-3-Phenylpropionyl)glycine Tricarballyl
    5-OH-indole-3-acetic Citramalic N-Acetyl-L-aspartic Vanilmandelic
    Xanthurenic

    Zivak Technologies 60 www.zivak.com


    F U T U R E O F A N A LY S I S

    6. MATERIALS SUPPLIED

    Order No. Volume Component


    ZV-3004-02R1-20 2 x 6 ml Reagent 1, Contains Internal Standards
    ZV-3004-02R2-20 1 x 6 ml Reagent 2, Contains acid
    ZV-3004-02R3-20 1 x 6 ml Reagent 3, Contains stabilizer
    ZV-3004-02MA-20 2 x 0,5 L Mobile Phase A, Contains organic solvent
    ZV-3004-02MB-20 1 x 0,5 L Mobile Phase B, Contains organic solvent
    ZV-3004-02WB-10 2 x 0.5 L Washing Solution, Contains organic solvent
    ZV-3004-KK-20 1 x 1 pc User Guide
    ZS-2ML-8-1001 2x100pcs 2 ml glass autosampler vial
    ZS-9004-0100-00 2x100pcs 2 ml glass autosampler vial caps pre-slit

    7. MATERIALS REQUIRED BUT NOT SUPPLIED

    Order No. Volume Component


    ZV-3004-02S1-20 1 x 3 ml Lyophilized Urine Calibrator Level 1
    ZV-3004-02S2-20 1 x 3 ml Lyophilized Urine Calibrator Level 2
    ZV-3004-02S3-20 1 x 3 ml Lyophilized Urine Calibrator Level 3
    ZV-3004-02K1-20 1 x 3 ml Lyophilized Urine Control Level 1
    ZV-3004-02K2-20 1 x 3 ml Lyophilized Urine Control Level 2
    ZV-3004-02C1-20 1 pcs Analytical Column

    TEST PROCEDURE
    Sample Pre-treatment (Manual)

    Vortex Vortex

    300 µl 50 µl R1 50 µl
    inject
    Sample R2+R3 mix

    LC-MS/MS PARAMETERS

    Ionization mode ESI positive + ESI negative


    Flow 0.25 ml/min
    Injection volume 20 µl

    00.00 100% A 0% B
    14.00 0% A 100% B
    Pump program 16.00 0% A 100% B
    16.01 100% A 0% B
    20.00 100% A 0% B

    Zivak Technologies 61 www.zivak.com

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