UNIT 2: GOLABORATORY PRACTICES

CHEM 101M/ 1ST SEM/ 1ST YEAR

Good laboratory practices (glp) → Whenever applicable, lubricate before using

glass stopcocks in burettes and separatory
Sets of carefully validated and approved funnels.
procedure that is implemented to assure quality and → Use a hood when conducting a reaction that
reliable results as well as SAFETY in the laboratory. could result in an explosion. Close the hood sash
to provide a shield.
WHAT IS A SCIENCE LABORATORY?
GENERAL HAZARD SYMBOLS
It is a room designed for experimental work where
students can validate the application of scientific about the chemicals and its hazards
concepts.
GENERAL LABORATORY RULES
→ No open toed shoes or flip-flops.
→ No shorts or loose-fitting clothes.
→ Use clips and hair nets to keep your hair in place
→ Remove rings, watches, and jewelries before Corrosive
performing an experiment. → These substances destroy living tissue and
→ Know what to do during emergency situations equipment upon contact.
→ Know hot to read and interpret Material Safety
Data Sheet (MSDS). Remember! Examples
→ Wear personal protective equipment, as Do not breathe vapors; HCl, sodium
appropriate. avoid contact with skin hypochlorite, sodium
and eyes. hydroxide
→ Wear lab gowns or lab coats whenever
performing an experiment.
→ Wear eye protective goggles.
→ Do not wear contact lenses.
→ Prescription glasses do not count as safety
goggles.
→ Wear the right kind of gloves when handling Oxidizer
corrosive reagents like acids as well as hot
containers. → Oxidizing substances can ignite flammable and
→ Do not eat, drink, or use tobacco products in the combustible material.
laboratory. Remember! Examples
→ Never work alone in the laboratory. Keep away from Hydrogen peroxide,
→ Keep lab doors closed; however, there are flammable, combustible calcium hypochlorite,
instances wherein experimental work produce and spontaneously ammonium nitrate
too much fumes which may require opening of combustible materials
the lab doors.
→ Hallways, corridors, and exit ways must be kept
clear. Do not locate (even temporarily)
laboratory equipment or supplies in these areas.
→ Allow proper storage for flammable and waste
materials.
→ Provide sufficient shelves or cabinets. Do not
store chemicals, apparatus, or equipment on the
floor.
→ Use only equipment that id free from flaws such
as cracks, chips, and obvious defects.
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UNIT 2: GOLABORATORY PRACTICES
CHEM 101M/ 1ST SEM/ 1ST YEAR

Poisonous Substances Remember!

→ Very hazardous to health when inhaled, Avoid contact with the human body and
swallowed or when they come in contact with the immediately consult a physician in case of contact
skin. May even lead to death. Radioactive

Remember! Examples → This symbol designates those substances which have

Avoid contact with the Cyanides, arsenic measurable radioactivity.
human body and substances
Remember!
immediately contact a
Avoid exposure
physician in case of
contact.

Harmful if swallowed

Flammable → Irritating (i) to skin: Limited evidence of a

carcinogenic effect.
→ Flammable Liquids: Flash point below 141F → Harmful (n): Danger of serious damage to health
(60.5C). by prolonged exposure through inhalation and if
→ Flammable Liquids: Keep away from open fires, swallowed.
sources of heat and sparks.
→ Combustible Material – Flashpoint > 141F but <
200F (or > 60.5C but > 90C)
Remember!
Keep away from open flames, sources of heat Very Toxic to Aquatic Organisms
and sparks.
→ May cause long-term adverse effects in the aquatic
environment.

Keep Away from Food

Explosives
→ Store away from foodstuffs. Toxic or poisonous
materials which are hazardous to health when → Considered all products and preparations, if they
inhaled, swallowed or when they come in contact explode through ignition or if they are more
with skin. sensitive than dinitrobenzene towards blows and
friction
Remember!
Avoid impact, knocks, frictions, sparks, heat, and
fire.
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UNIT 2: GOLABORATORY PRACTICES
CHEM 101M/ 1ST SEM/ 1ST YEAR
GENERAL RULES OF SAFE STORAGE OF HEALTH
CHEMICALS
“an ounce of prevention is better than a pound of cure”
→ Dangerous goods must be stored under special
safety condition to avoid health and → Wear proper PPE.
environmental disaster. → Inform professors/instructors/lab technician about
→ It does not matter how many. health concerns.
→ Separate the incompatible materials. → Be cautious with your own health – allergic response
→ If the substance has several hazard degrees, it to chemicals.
should be classified as per its highest one: → If on medication inform your professor/instructor/lab
EXPLOSIVE > OXIDIZING > FLAMMABLE > TOXIC > technician & bring your own health aid kit if
CORROSIVE > HARMFUL necessary.
GENERAL RULE → Determine the locations of lab first aid kits &
university clinic.

WASTE CLASSIFICATION
→ Waste Receptacles
→ Garbage Bins
→ Broken Glass Container
→ Cleanliness

WORKING ATTITUDE
→ Patience
→ Time conscious
→ Honesty
→ Cleanliness & neatness
→ Achievement driven & focused
→ Resource Management (chemical usage, water,
electricity)
→ Respect
→ Diligence & Proactive
→ Independence

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