INSTRUCTIONS

BX53/51/41-P
POLARIZING MICROSCOPE

This instruction manual is for the Olympus Polarizing Microscope. To obtain optimum performance and
to familiarize yourself fully with the use of your microscope, we recommend that you read this manual
together with the instruction manual for the BX53/51/41 microscope thoroughly before use. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
AX7988
 BX53/51/41-P
CONTENTS
IMPORTANT — Be sure to read this chapter for safe use of the equipment. — 1

1 NOMENCLATURE 2

2 ASSEMBLY 3-9
2-1 Assembly Diagram ......................................................................................................................................................................................................................................... 3
2-2 Detailed Assembly Procedure ................................................................................................................................................................................................ 4-9
3 CONTROLS 10-11

4 USING THE CONTROLS 12-15

4-1 Stage ......................................................................................................................................................................................................................................................................... 12-14
4-2 Intermediate Polarizing Attachment ...................................................................................................................................................................................... 15

5 POLARIZED LIGHT OBSERVATION 16-26

5-1 Adjustments Before Observation .................................................................................................................................................................................... 16-24
1 Adjusting the Optical Axis .......................................... 16-21 2 Adjusting for Extinction ............................................................. 22
3 Adjusting the Eyepiece Cross Lines .................................................................................................................................................................................... 23,24
5-2 Orthoscopic Observation ..................................................................................................................................................................................................................... 25
5-3 Conoscopic Observation ...................................................................................................................................................................................................................... 26
6 SPECIFICATIONS 27-28

7 OPTICAL CHARACTERISTICS 29

8 TROUBLESHOOTING GUIDE 30
 IMPORTANT
The BX53/51-P and BX41-P have different combinations of microscope frames and stages.

Module BX53/51-P BX41-P

Microscope frame BX53F/BX51TF BX41TF
Stage U-SRP U-SRG2

1 Getting Ready
1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
2. The BX53/BX51/BX41 microscope can be used with up to two additional intermediate attachments (e.g., U-DO3 dual-
viewing attachment, U-CA or U-ECA magnification changer, etc.). When using an additional intermediate attachment,
please make sure with your Olympus representative or the latest brochure.
3. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations.
Install it on stable, level bench. For operation environment conditions, see SPECIFICATIONS on page 28.
4. When disposing of the microscope, check the regulations and rules of your local government and be sure to observe them.

2 Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury or damage to the equipment or other property. It may also be used
to alert against unsafe praclices.
} : Indicates commentary (for ease of operation and maintenance).

1
 BX53/51/41-P
NOMENCLATURE
Eyepiece
Observation tube

BX53/51 Microscope
frame, or
BX41Microscope frame
* Polarizing intermediate
attachment

* Objective
* Centerable revolving (Strain-free objective series)
eyepiece

* Polarizing rotatable
stage, or
Rotatable stage

* Strain-free Lamp housing

condenser

*Components for polarized light observation

2
 ASSEMBLY
2-1 Assembly Diagram
The diagram below shows how to assemble the various components. The numbers indicate the order of assembly.
}For details on the BX53/51/41 microscope frame, consult the BX53/51/41 manual.
CAUTION When assembling the components, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.
Eyepiece Observation tube

Centerable revolving
nosepiece (U-P4RE)
Intermediate attachment
for AN360P (U-OPA)

Test plate adapter Rotatable analyzer

(U-TAD) Strain-free Polarizing (U-AN360P-2)
objective intermediate
Sensitive tint plate attachment 100W halogen lamp
(U-TP530) Mechanical stage (U-CPA) housing
Quarter wave plate (U-FMP) 100W
(U-TP137) * halogen
bulb

Stage clips

Polarizing lamp sooket

rotatable stage
(U-SRP)
Rotatable stage
(U-SRG2)
Power cord
Microscope frame
*6 types of compensators available Strain-free condenser BX53F
(U-POC-2) BX51TF
BX41TF
3
 BX53/51/41-P

2-2 Detailed Assembly Procedure

1 Attaching the Stage (U-SRP) (Fig. 1)

1. Turn the coarse adjustment knob @ to lower the substage assembly ² to

³ its lower limit.
| 2. Loosen the centering knobs ³.
3. Position the stage with the vernier scale | in front, and carefully lower the
stage onto the round dovetail on the stage bracket ² with the positioning
pin on the stage aligned with the groove on the front of the stage bracket,
² then loosely tighten the centering knobs.
@

Fig. 1

Mounting Stage Clips and Mechanical Stage (U-FMP) (Fig. 2)

ƒ
· Insert the stage clips securely into any two holes ƒ on the stage top
surface.
· Mount the mechanical stage so that the positioning pins on the under-
side fit into the positioning holes … on the stage top surface. Using the
Allen screwdriver provided with the microscope frame, tighten the clamping
screw.

…
Fig. 2

4
 2 Mounting the Condenser (U-POC-2) (Fig. 3)

1. Turn the coarse adjustment knob @ to raise the stage to its upper limit.
² 2. Turn the condenser height adjustment knob ² to lower the condenser
holder to its lower limit.
3. Loosen the condenser clamping screw ³.
4. Position the condenser with the scale markings in front, insert it into the
substage fork as far as it will go.
Align the positioning pin at the back of the condenser with the groove in
@
³ the substage fork.

Fig. 3 CAUTION Swing the top lens out of the way before inserting the condenser.
5. Tighten the condenser clamping screw, then raise the condenser to its
upper limit.

5
 BX53/51/41-P

3 Mounting the Objectives (Fig. 4)

Low
Insert the 10X or 20X objective into the primary hole (position where black
rubber plugs are inserted into the centering holes on the nosepiece).
Mount the other objectives in such a manner, that the magnification in-
creases from low to high power in a clock-wise direction from the primary
High
hole.

Fig. 4

@ 4 Mounting the Revolving Nosepiece (U-P4RE) (Fig. 5)

1. Turn the coarse adjustment knob to lower the stage all the way.
2. Using the Allen screwdriver, loosen the nosepiece clamping screw @ on
the microscope frame.
3. Carefully slide the nosepiece along the dovetail to its limit, in the direction
of the arrow.
4. Clamp the nosepiece by tightening the nosepiece clamping screw.

Fig. 5

6
 5 Mounting the Intermediate Attachments (Fig. 6)

@ 1. Using the Allen screwdriver, loosen the observation tube clamping screw
@ on the microscope frame.
2. Insert the circular dovetail mount at the bottom of the intermediate
attachments into the opening on the microscope frame and clamp
by tightening the clamping screw @.

When Using Intermediate Attachment for conoscopic and orthoscopic

observation (U-CPA)
Fig. 6 CAUTION Always make sure to mount this unit in such a manner, that it is
parallel to the arm.
Intermediate Attachment for orthoscopic observation (U-OPA)
}The position of this intermediate tube can be adjusted later. At this point,
place the tube so the nameplate is toward the rear.

7
 BX53/51/41-P

6 Mounting the Observation Tube (Fig. 7)

1. Using the Allen screwdriver, fully loosen the observation tube clamping
@ screw @ on the intermediate attachment.
2. Insert the circular dovetail mount at the bottom of the observation tube
into the opening on the intermediate attachment, placing the observation
tube to point the binocular eyepieces towards the front. Fasten the
observation tube by tightening the clamping screw @.

7 Mounting the Eyepieces

Fig. 7 Insert the eyepiece with cross lines into the right hand eyepiece sleeve.
Make sure the eyepiece is inserted so that the positioning pin on the
eyepiece is inserted into the groove at the lower end of the sleeve.

8 Mounting the Test Plate Adapter (U-TAD) (Fig. 8)

1. Loosen the revolving nosepiece clamping knob @ and remove the

² dummy slider.
@ 2. Insert the test plate adapter ² and securely tighten the clamping knob
@.

Fig. 8

8
 9 Mounting Rotatable Analyzer (U-AN360P-2) (Fig. 9)

1. Place a desired ND filter (30 mm diam.) in the empty hole @ as required.

2. Insert the rotatable analyzer (U-AN360P-2) ² as far as the click-stop
position. Then screw in the stopper knob ³. (Fig. 10)
When using the fixed analyzer U-ANT instead of the rotatable analyzer
U-AN360P-2, place the fixed analyzer in the test plate adapter U-TAD.
Position the fixed analyzer so that the positioning pin on the fixed
analyzer fits into the groove. The fixed analyzer will be held in place by
@ ² a magnet.

Fig. 9

10 Mounting Test Plate Compensator (Fig. 10)

1. Loosen the test plate adapter (U-TAD) clamping knob @.

2. Insert the sensitive tint plate (U-TP530), quarter wave plate (U-TP137),
or any of the other compensators (6 types) into the test plate adapter,
and securely tighten the clamping knob.
³

Fig. 10

9
 BX53/51/41-P
CONTROLS
The illustration shows the U-CPA
set. Other components are intro-
duced on the following pages.

Bertrand lens focusing knob

Bertrand lens knob

Slider clamping knob Analyzer

Slot for compensators

Mechanical stage

Vernier scale

Stage rotation
Aperture iris diaphragm lever clamping knob

Polarizer clamping knob

Polarizer rotation ring

10
 Intermediate Adapter for orthoscopic Rotatable Analyzer (U-AN360P-2)
observation (U-OPA)
Empty hole
(ND filter insertion possible)

Clamping knob
Analyzer slot

Stopper knob

Rotation dial

Alignment polarizer

Sensitive Tint Plate

Rotatable Stage (U-SRG2)
(U-TP530)
Test Plate Adapter
(U-TAD)
U-FMP mounting Centering knob
screw holes
Test plate clamping knob

Quarter Wave Plate Stage rotation

Hole for the (U-TP137) clamping knob
fixed analyzer
(U-ANT) Stage clips

Slot for compensator plate Stage center plate Clamping screw

11
 BX53/51/41-P
USING THE CONTROLS
4-1 Stage
1 Specimen Placement

When Using the Stage Clips (Fig. 11)

Position the specimen in the center, and clamp the specimen with the
stage clips.
When Using the Mechanical Stage (U-FMP) (Fig. 12)
Open the spring-loaded finger @ and place the specimen on the stage.
CAUTION Applicable slide glass is for inorganic substances (28 x 48
mm) and organic substances (26 x 76 mm) with cover glass
Fig. 11
thickness of 0.17 mm.

Inorganic specimen

Organic specimen

Fig. 12

12
 2 Stage Rotation (Fig. 13)
@ When the stage rotation clamping knob @ is loosened, the stage can be
rotated horizontally through 360°.

Fig. 13

3 Using 45° Click Stop Lever (U-SRP only) (Fig. 14)

When the 45° click stop lever @, located at the right side of the stage, is
moved toward the observer, and the stage is moved from this position to
the first click-stop position, the specimen is moved 45° to its diagonal
position. To release the 45° click stop function, push back the lever.
CAUTION When releasing the 45° click stop function by pushing back the
lever, do so at a click-stop position.

@
Fig. 14

13
 BX53/51/41-P

@ 4 Stage Height Adjustment (Figs. 15 & 16)

}By lowering the position of the substage, the microscope will accom-
modate specimens with a maximum height of 35 mm. This is useful
when observing metallurgical specimens and other thick objects.
1. Lower the stage to its lower limit, then remove the stage from the
microscope. (See page 4.)
2. Using the Allen screwdriver, loosen the substage bracket clamping
screw @ and remove the substage assembly. (Fig. 15)
3. Turn the coarse adjustment knob and raise the focusing block ³ to
where the stopper screw ² in the arm becomes visible. (Fig. 16)
Fig. 15
4. Using the Allen screwdriver, loosen and remove the upper stopper
screw ².
² ³ 5. Reattach substage assembly and stage.
}Store the remove stopper screw ² in a safe place so that it will not be
lost, if needed again.

Fig. 16

14
 4-2 Intermediate Polarizing Attachment
1 Using the Bertrand Lens (U-CPA only) (Fig. 17)

By manipulating the Bertrand lens knob @ on the front, the Bertrand

lens is inserted into or removed from the light path. At the pushed-in
({) position, the lens is engaged. At the pulled-out (¦) position, the
lens is removed from the light path.
@

Fig. 17

2 Using the Analyzer (Fig. 18)

The analyzer @ is engaged when pushed in to the second click-stop

position. To disengaged the analyzer, and to place the empty hole in
the light path, pull the analyzer out to the first click-stop.

² 3 Focusing the Conoscopic Image (U-CPA only) (Fig. 18)

@ To focus the conoscopic image, manipulate the Bertrand lens focusing

knob ². While observing the conoscopic image, move the knob to
find the position where image is sharply focused.
Fig. 18

15
 BX53/51/41-P
POLARIZED LIGHT OBSERVATION
5-1 Adjustments Before Observation
In polarized light microscopy ultimate performance is not obtainable unless optical adjustments are made correctly.
Always performed the following adjustments before observation. Remove the quarter wave plate and sensitive tint plate
from the light path.

1 Adjusting the Optical Axis

When Using the Intermediate Attachment for orthoscopic observation

³ (U-OPA) (Figs. 19 & 20)

² CAUTION Make sure the 10X objective is inserted into the primary hole on
the centerable revolving nosepiece.
@ 1. Fully insert the alignment polarizer @, provided with the U-OPA into
the test plate adapter (U-TAD). Tighten its clamping knob. (Fig. 19)
2. Remove the condenser.
Fig. 19 3. Insert the rotatable analyzer (U-AN360P-2) ² into the analyzer slot on
the intermediate attachment. Engage the analyzer, loosen the clamping
screw and set the analyzer’s rotation dial ³ to 0°. (Fig. 19)

16
 4. Slightly loosen the intermediate adapter clamping screw ƒ. (Fig. 20)
5. While observing the field of view, rotate the intermediate attachment for
| orthoscopic observation (U-OPA) | until complete extinction is obtained.
At this point, tighten the intermediate attachment clamping screw ƒ.
(Fig. 20)
6. Remove the provided alignment polarizer.
7. Attach the condenser.
8. Center the condenser. (For details, see instructions of each microscope.)
ƒ

Fig. 20

9. Centering the rotatable stage (Figs. 21 & 22)

(1) Place the specimen.
† …
(2) Focus on the specimen and look for an easily recognizable detail …
in the field. Move this detail in the center of the eyepiece cross lines.
(3) When the stage is rotated, the detail moves in circle †. Manipulate
the two stage centering knobs ‡ to coincide the imaginary center of
the circle † circumscribed by the detail with the intersection of the
eyepiece cross lines.
(4) Moving the specimen only, move a new specimen detail into the
cross line center.
}Repeat steps (3) and (4) several times until the center of the stage
Fig. 21
rotation is in the center of the cross lines, i.e., when rotating the stage,
the specimen stays in the center of the cross lines.

17
 BX53/51/41-P

(5) Clamp the stage with the stage rotation clamping knob.

‡ 10. This concludes centering of the optical axis for the 10X objective, which
will act as the reference objective. Now, center the other objectives with
the centerable nosepiece by engaging the objectives into the light path
one after another.

Fig. 22

When Using Intermediate Attachment for conoscopic and orthoscopic

observation (U-CPA)
· Normal Observation

CAUTION Make sure the 10X objective is inserted into the primary
hole on the centerable revolving nosepiece.
@ 1. Pull out the Bertrand lens knob @ to the OUT (¦) position. (Fig. 23)
2. Center the condenser. (For details, see instructions of each microscope.)
3. Follow steps 9 and 10 in “When Using the Intermediate Attachment
for orthoscopic observation".
Fig. 23

18
 Note: During conoscopic observation, the center of the conoscopic image
@ and the intersection of the cross lines ² may not coincide.
Cross lines ²
However, employment of the universal infinity optical design ensures
that this disagreement will have no measurable effect during actual
observation.
However, if the disagreement is distracting, adjust in the same manner
as when using the U-CPA (for photomicrography), described in the
following paragraph.
Conoscopic image @

Fig. 24

When Using Intermediate Attachment for conoscopic and orthoscopic

observation (U-CPA)
· Photomicrography
1. Using the tip of a mechanical pencial, etc., push out, in the direction of
the objective hole, the black rubber plugs inserted into the centering
@ holes of the primary hole where the 20X or 10X objective is inserted.
2. Pull out the Bertrand lens knob @ to the OUT (¦) position. (Fig. 25)
3. Adjust the position of the intermediate tube (Refer to page 7.)
4. Center the condenser. (For details, see instructions of each microscope.)
5. Push in the Bertrand lens knob @ to the IN ({) position for conoscopic
Fig. 25
observation.

19
 BX53/51/41-P

6. To brighten and facilitate observation of the conoscopic image during

optical axis adjustment, rotate the analyzer slightly away from the position
of total extinction. (Fig. 26)

Conoscopic image

Fig. 26

7. Insert the two provided centering wrenches ² into the centering holes
for the primary hole with the 20X or 10X objective on the nosepiece.
8. Manipulate the centering wrenches ² to bring the bright center portion
of the conoscopic image into the center of the field of view (Fig. 27)
9. Pull out the Bertrand lens knob to the OUT (¦) position. At this state,
center the condenser again, as in step 4 above.

²
Fig. 27

20
 10. Centering the rotatable stage (Figs. 28 & 29)
(1) Place the specimen.
| ³ (2) Focus on the specimen and look for an easily recognizable detail ³
in the field. Move this detail in the center of the eyepiece cross lines.
(3) When the stage is rotated, the detail moves in circle |. Manipulate
the two stage centering knobs ƒ to coincide the imaginary center of
the circle | circumscribed by the detail with the intersection of the
eyepiece cross lines. Depending on the degree of the stage
decentration, this will move the specimen detail in the opposite direc-
tion, away from the cross line center.
(4) Moving the specimen only, move a new specimen detail into the
Fig. 28
cross line center.
}Repeat steps (3) and (4) several times until the center of the stage
rotation is in the center of the cross lines, i.e., when rotating the stage, the
ƒ specimen detail stays in the center of the cross lines.
(5) Clamp the stage with the stage rotation clamping knob.

11. This concludes centering of the optical axis for the 20X or 10X objective,
which will act as the reference objective. Now, center the other objectives
with the centerable nosepiece by engaging the objectives into the light
path one after another.

Fig. 29

21
 BX53/51/41-P

2 Adjusting for Extinction (Fig. 30)

CAUTION Remove specimen, test plate, compensator, etc. from the light
path.
1. Swing out the top lens of the condenser, and engage the 10X objective.
2. Insert the rotatable analyzer into the light path and set vibration direction
scale at the 0° position. Clamp with the clamping knob. (Fig. 30)
3. Set the polarizer scale at the 0° position.
4. Loosen the polarizer clamping knob @. Rotate the polarizer rotation
² @ dial ², until complete extinction is obtained. At this point, tighten the
Fig. 30 clamping knob @. (Fig. 30)

22
 3 Adjusting the Eyepiece Cross Lines (Fig. 31)

}To align the eyepiece cross lines and the vibration direction, the
orientation plate provided with the U-CPA or U-OPA is required.

Orientation plate (U-PJ: Single module name)

Fig. 31 CAUTION Remove the test plate adapter and the compensator from the
light path.
1. Swing out the top lens of the condenser.
2. Place the orientation plate on the stage. Remove the analyzer from the
light path, and use the 4X objective to focus.

23
 BX53/51/41-P

3. Coincide the orientation plate’s center portion with the intersection of the eyepiece cross lines. Engage the analyzer into
the light path (in case of U-AN360P-2, set the analyzer to position 0°) to obtain crossed filters position (extinction).
4. While observing, rotate the stage to locate the position where the orientation plate is darkest. At this point, clamp the
stage.
5. Remove the analyzer from the light path. Set to brightfield, and slightly loosen the observation tube clamping screw.
6. Rotate the observation tube to position the eyepiece cross line parallel to the fiducial line of the orientation plate. Tighten
the observation tube clamping screw.

Orientation Eyepiece
plate cross lines

Parallel
position

Fiducial line

24
 5-2 Orthoscopic Observation
}In principle, polarized light enters the light path, parallel to the optical axis, to enable observation of the optical
characteristics of the specimen. Therefore, swing out the top lens of the condenser. Use 4X to 100X objectives.
1. When using the intermediate attachment conoscopic and orthoscopic observation U-CPA, pull out the Bertrand
lens knob to remove the Bertrand lens from the light path.
2. Insert the analyzer and perform observation.
CAUTION · When the top lens is swung out, the aperture iris diaphragm and field iris diaphragm will not function nor-
mally.
· If the aperture iris diaphragm is stopped down, the field of view may be restricted.
3. Insert a test plate (sensitive tint plate (U-TP530), quarter wave plate (U-TP137)) into the test plate slot. When fully
inserted, the plate is engaged. To remove the test plate from the light path, pull out to first click-stop position.
For details on other compensators, see the separate instruction manuals.

25
 BX53/51/41-P

5-3 Conoscopic Observation

}Use 20X to 100X objectives.
1. Engage the analyzer and adjust for extinction position.
2. Swing the condenser top lens into the light path.
3. When using the intermediate attachment conoscopic and orthoscopic
observation U-CPA, push in the Bertrand lens knob to engage the Bertrand
lens into the light path.
4. Engage an objective of your choice (20X to 100X).
5. Open the aperture iris diaphragm.
6. Slide the focusing knob on the U-CPA to focus on the conoscopic image.
}When the intermediate attachment conoscopic and orthoscopic obser-
vation U-CPA is not used, conoscopic observation is possible by remov-
Fig. 32
ing an eyepiece from the observation tube and looking at the rear focal
plane of the objective directly.
}To obtain good image contrast, place an interference filter (45IF546) in
the filter holder on the light exit of the microscope.
}If the periphery of the conoscopic image is dark, move the condenser
vertically to find the position where the periphery is brightest.

26
 SPECIFICATIONS
Specifications
Item Intermediate attachment conoscopic and Intermediate attachment for
orthoscopic observation (U-CPA) orthoscopic observation (U-OPA)
1. Polarizing intermediate Field No. 22
attachment
(U-CPA and U-OPA) Bertrand lens Focusable
Bertrand aperture stop Fixed diaphragm
Bertrand lens knob Pushed in
position for switch position: { IN
between orthoscopic Pulled out
and conoscopic position: ¦ OUT
observation
Analyzer slot Slot for rotatable analyzer (U-AN360P-2)
2. Analyzer 360° dial rotation
(U-AN360P-2) Minimum scale reading: 0.1° (Vernier scale)
3. Revolving nosepiece Type: Centerable quadruple
(U-P4RE) Attachable compensators: Quarter wave plate (U-TP137), sensitive tint plate (U-TP530)
and all kinds of compensators by means of test plate
adapter (U-TAD).
4. Stage (U-SRP) Type: Polarizing rotatable stage with 3-point centering mechanism 360° horizontal
rotation, clamps at any desired position
360° scale (minimum division:
1°; minimum reading 6' by means of vernier scale)
45° click stop knob
· Stage clips (U-SCB2) attachable for holding specimen in place
· Attachable mechanical stage (U-FMP)
· Universal stage (ZEISS Co. manufacture) attachable

27
 BX53/51/41-P

Specifications
Item Intermediate attachment conoscopic and Intermediate attachment for
orthoscopic observation (U-CPA) orthoscopic observation (U-OPA)
5. Stage (U-SRG2) Type: Rotatable stage with 3-point centering mechanism 360° horizontal rotation,
clamps at any desired position
360° scale (minimum division: 1° reading)
· Stage clips (U-SCB2) attachable for holding specimen in place
· Attachable mechanical stage (U-FMP)
6. Condenser (U-POC-2) Aplanatic/achromatic condenser, top lens swing-out type
Polarizer 360° rotatable
0° position adjustable
N.A . 0.9 (top lens swung in)
Applicable objectives: 2X to 100X (2X to 4X objectives are used with the top lens
swung out)

Operating environment · Indoor use

· Altitude up to 2000 m
· Temperature: 5° to 40°C (41° to 104°F)
· Maximum relative humidity 80% for temperatures up to 31°C (88°F)
decreasing linearly to 50% relative humidity at 40°C (104°F)
· Main supply voltage fluctuations not to exceed ±10% of the nominal voltage
· Installation/Overvoltage Category II (In accordance with IEC60664)
· Pollution Degree 2 (In accordance with lEC60664)

28
 OPTICAL CHARACTERISTICS
– UIS series objectives not listed here can also be combined with this microscope. –

The table below shows the optical characteristics of different Designation

eyepiece and objective combinations. Objective specifications
Magnification
are marked on the objective (as shown in the diagram on the Numerical aperture (N.A.)
right).
Mechanical
tube length Field number (FN)
NOTE
Refer to the latest catalogue or consult your local Olympus Cover glass thickness
Color band
representative for the updated information on the eyepieces
and objectives that can be combined with this microscope.

Optical Eyepiece WHN10X (FN22)

character Cover glass Resolution
Magnifi- W.D.
cation N. A. (mm) thickness (μm) Depth Remark
(mm) Field
UIS2 Total mag. of focus
of view
objective (μm)
PLN-P
Plan Achromat
for polarized light 104X 0.10 18.5 – 3.36 0140X 180.00 5.50
(FN22)
ACHN-P 010X 0.25 06.0 – 1.34 0100X 28.00 2.20
Achromat 120X 0.40 03.0 0.17 0.84 1200X 6.09 1.10
for polarized light 140X 0.65 00.45 0.17 0.52 1400X 3.04 0.55
(FN22) 100XO 1.25 00.13 0.17 0.27 1000X 0.69 0.22 Oil immersion
UPLFLN-P 104X 0.13 017.0 – 2.58 0140X 83.60 5.50
Plan Semi 010X 0.3 010.0 – 1.12 0100X 14.70 2.20
Apochromat for 120X 0.5 02.1 0.17 0.67 1200X 4.60 1.10
polarized light 140X 0.75 00.51 0.17 0.45 1400X 1.66 0.55
(FN26.5) 100X 1.3 00.20 0.17 0.26 1000X 0.43 0.22 Oil immersion

29
 BX53/51/41-P
TROUBLESHOOTING GUIDE
Under certain conditions, performance of this unit may be adversely affected by factors other than defects. If problems
occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking
the entire list, please contact your local Olympus representative for assistance.

Trouble Cause Remedy Page

1. Optical System
a. Bulb is lit, but field of view remains Bertrand lens is engaged. Remove the Bertrand lens from the
15
dark. light path.
In the extinction condition. Remove the analyzer from the light
15
path.
b. Vignetting or uneven field of view. Test plate is stopped at an intermediate Set at click-stop position.
9
position.
During orthoscopic observation, the con- Remove top lens completely from the
denser top lens is placed in the light path light path. –
or stopped at intermediate position.
c. The conoscopic image is not visible. Condenser top lens is not placed in Swing in the top lens. –
the light path.
Bertrand lens is not placed in the light path. Place Bertrand lens in the light path. 15
Intermediate attachment for ortho- Replace it with the U-CPA if you have
scopic observation (U-OPA) is attached. one. If you don’t, look into the eyepiece
7,26
sleeve to observe the conoscopic im-
age.
d. Extinction is not obtainable. The analyzer is not in the light path. Engage the analyzer. 9

30
MEMO
Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan

Wendenstrasse 14-18, 20097 Hamburg, Germany

3500 Corporate Parkway, Center Valley, Pennsylvania 18034-0610, U.S.A.

491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373

31 Gilby Road, Mount Waverley, VIC., 3149, Melbourne, Australia

5301 Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.

Printed in Japan on October 05, 2010 M 005–01