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    En Nws Oct 20026

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    aryan

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    En Nws Oct 20026

    Uploaded by

    aryan
    23 views1 page

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    23 views1 page

    En Nws Oct 20026

    Uploaded by

    aryan

    Copyright:

    © All Rights Reserved
    Download as TXT, PDF, TXT or read online from Scribd
    Download as txt, pdf, or txt
    of 1

    Novel approaches to assess the microbiological quality of bathing water

    21.10.2020

    In his doctoral thesis, Ananda Tiwari, M.Sc., tested the performance of the current
    bathing water monitoring approaches and utilized novel molecular methods and high-
    throughput sequencing to unveil fecal pollution sources and bacterial communities
    in surface water resources around Finland. 
    Rapid and precise monitoring methods of water quality are needed to secure the
    health of water users and for assessing waterborne human health risks. As part of
    his PhD studies, Tiwari validated the use of most-probable number method based on
    defined substrate technology (Colilert-18, currently described as international
    standard ISO 9308-2) as an alternate method for counting Escherichia coli at
    Finnish bathing sites. Further, he defined for the first time the categorical
    performance characteristics of the ISO 7899-2 standard method for enumeration of
    intestinal enterococci, the method used for water quality investigations
    worldwide. 

    The experimental part of the work highlighted that aquatic plants and superficial
    sediments may change the decay of enteric microbes in bathing sites. Further,
    differential microbial community profiles were noted when surface water samples
    collected during winter and summer were compared. Favorable conditions for microbes
    could be created with reduced solar radiation, increased nutrient concentrations
    and lower ambient temperature in bathing water. Such conditions, if present, favor
    the longer persistence of enteric microbes or even support the growth of certain
    pathogenic bacteria such as Vibrio cholerae. In this study, intestinal enterococci
    decayed faster in water than in the sediment or vegetation. 

    The current bathing water monitoring involves enumerating Escherichia coli and
    intestinal enterococci as faecal indicator bacteria. These bacteria are found in a
    wide range of hosts mostly in the feces of all warm-blooded animals and humans.
    Thus, such monitoring cannot differentiate the source of fecal contamination. In
    this study, the enumeration of the intestinal enterococci counts alone was not
    enough for predicting pathogens, such as enteric viruses and Vibrio spp. in bathing
    water. To properly address the bathing water related public health risks, there is
    a need for applying the available novel approaches in the risk assessment
    procedures. This study proposes the use of quantitative biomarker gene detection
    technologies for microbial source tracking and high-throughput 16S rRNA gene
    sequencing for microbial community profiling. 

    The doctoral dissertation of MSc Ananda Tiwari entitled Monitoring Microbial


    Quality, Contaminant Sources and Bacterial Communities of Bathing Water in Finland
    will be examined at the Faculty of Science and Forestry. The opponent in the public
    examination will be Docent Rauni Kivistö, University of Helsinki and the custos
    will be Chief Specialist, Associate Professor Tarja Pitkänen, Finnish Institute for
    Welfare and University of Helsinki. The public examination will be held in English.

    Online event

    Photo available for download

    Electronic version of the doctoral dissertation

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