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    Chromatography Resins For Protein Purification

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    Melisa L Castrillon

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    Chromatography Resins For Protein Purification

    Uploaded by

    Melisa L Castrillon
    63 views2 pages

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    Chromatography Resins for Protein Purification

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    Copyright:

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    63 views2 pages

    Chromatography Resins For Protein Purification

    Uploaded by

    Melisa L Castrillon

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Contact Us: www.pall.

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    Chromatography Resins for Protein Purification


    Chromatography Resins for Protein Purification

    Pall Chromatography Resin Sections


    Introduction
    Ceramic HyperD® F Ion Exchange Resin
    Blue Trisacryl® M Resin
    HA Ultrogel® Resin
    MEP HyperCel Resin for Purification of Antibodies
    Heparin HyperD M Resin
    Lysine HyperD Resin
    HEA and PPA HyperCel
    HyperCel STAR AX

    Chromatography Resins Introduction

    Chromatography continues to be an essential technology for the


    purification of biomolecules. Pall offers a line of chromatography
    resins ideal for protein purification applications (see Table 4.6). This
    broad line of chromatography products exhibits superior
    performance and is useful for affinity, ion exchange, size exclusion,
    and hydrophobic interaction chromatography (HIC). Unique mixed-
    mode sorbents also exist to provide solutions to current sample
    preparation challenges.

    The resins Pall offers for small-scale applications are the same ones
    offered to our customers currently manufacturing
    biopharmaceuticals. The ability to scale up is essential for those
    working in drug discovery, development, and manufacturing. These
    resins can be used in varying size chromatography columns, as well
    as in batch mode for single or high throughput mode. This is ideal
    for quick preps or in situations where optimizing purification
    conditions is required.

    Table 4.6
    Chromatography Resins

    Chromatography Type Product Name Description


    Ion Exchange Q Ceramic HyperD ® F Strong anionic exchanger,
    binds negatively-charged target

    CM Ceramic HyperD F Weak cationic exchanger


    DEAE Ceramic HyperD F Weak anionic exchanger

    HyperCel STAR AX Weak “salt tolerant” anionic exchanger

    Affinity Blue Trisacryl® M Binds albumin


    Heparin HyperD M Direct binding to targets that
    have an affinity for heparin

    Lysine HyperD Direct binding to targets that


    have an affinity for lysine
    SDR HyperD Detergent removal

    Mixed Mode MEP HyperCel Uses several binding mechanisms


    including hydrophobic interactions

    HA Ultrogel® Hydroxyapatite
    HEA HyperCel Hydrophobic and electrostatic interactions

    PPA HyperCel Hydrophobic and electrostatic interactions


    HCIC MEP HyperCel Uses several binding mechanisms
    including hydrophobic interactions

    Table 4.7
    Available Separation Columns

    Description Column Value Available from Pall


    Glass Chromatography Column 0 to 900 mL Yes

    Disposable Chromatography Column 1 mL and 5 mL Yes


    Spin Filter <1 mL Yes

    Deep Well Multi-Well Filter Plate 96 x <1 mL Yes


    Multi-Well Filter Plate 96 x <350 µL Yes

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