HPLC Columns

analytical hplc
Analytical HPLC Column

Introduction
Column Families Suitable for Small Molecules

(<2000 Molecular Weight)
hplc columns | introduction
Grace Key Column Families

Single-Source Solution for Discovery to Recovery
Applications
HPLC is commonly used in a wide range of applications,
including drug discovery and purification for the
pharmaceutical and biotechnology industries, environmental
analysis, forensics, petrochemical analysis, food, cosmetics,
and vitamins. The combined premier product lines of Alltech,
Davisil, Flexit, Grom, Jones Chromatography,
Modcol, and Vydac create a single-source solution for
HPLC columns and accessories from discovery to recovery.
Our column families include reversed-phase, normal-phase,
HILIC, ion-exchange, ion-exclusion, size-exclusion, and
affinity stationary phases for small- and large-molecule
separations, and our column formats maximize their
performance. Our product range includes standard and
custom columns for analytical separations, preparative
phases for scale up, bulk packings that customers can
pack in their own columns, and accessories to maintain
separation ruggedness and quality.
To help select the appropriate column for your application,
we describe key column families and highlight unique phases
within these families. Whether the most important factor
is analysis speed, column bleed, pH stability, resolution,
adjustable selectivity, or analyte molecular weight, Grace
offers a column to suit your application.
VisionHT Ultra High-Pressure Columns

12,000psig pressure-rated columns, with
sub 2m media for high-efficiency, high-speed
separations
VisionHT columns are optimized for high throughput
and ultra high-pressure LC applications. Mechanically
stable 1.5m media and ultra-low volume hardware
delivers new separation benefits with excellent stability.
A variety of phases available.
Differentiated Phases: C18, C10-B, C18-P, C18-HL,
HILIC, Silica
Specifications: Spherical silica, monomerically bonded,
proprietary endcapping, 100, 120 pore size
Formats: Ultra High-Pressure
Alltech Alltima HP Columns

Premium quality, exceptionally stable
columns, without phase bleed
Alltech Alltima HP phases are made from high purity
silica to eliminate peak tailing. Proprietary bonding
eliminates the problem of column bleed for MS and
ELSD detection. Full range of phases with pH stability
from 1 to 10.
Differentiated Phases: C18 for classic reversed-phase
separations, EPS for extended polar selectivity, C18
HiLoad for extra reversed-phase retention, C18 Amide
with low bleed, and HILIC.
Specifications: High purity spherical silica, monomerically bonded, endcapped, 100, 120, 190 pore size
Formats: Microbore, Expedite, Rocket, SolventReducer, Analytical, Prep
7110
more info
For information about Grace analytical hardware formats,

see page 31.
more info
For information about prep columns, see pages 148 171.
Alltech Prevail Columns

Reversed-phase columns for use with 100%
organic to 100% aqueous mobile phases
Prevail phases are designed for wettability with
aqueous and organic mobile phases, and are especially
useful for broad gradients. Retain highly polar analytes
with aqueous mobile phases as well as hydrophobic
analytes in organic mobile phases.
Differentiated Phases: Carbohydrate ES, Organic Acid
endcapped, 110 pore size
24
www.discoverysciences.com
analytical hplc
Analytical HPLC Column Introduction

Column Families Suitable for Large Molecules
(>2000 Molecular Weight)
Alltech Platinum Columns
Vydac MS Columns
Alternate retention and selectivity for

polar and nonpolar analytes available in
sub 2m particle sizes
Next generation Vydac media from the leader

in peptide and protein separations
Alltech Platinum phases have a controlled polar silica

surface which provides enhanced polar selectivity (EPS)
in addition to reversed-phase retention. C18 and EPS
C18 columns are a complimentary duo to separate polar
and nonpolar analytes.
Vydac MS columns provide unique selectivity and

exceptional protein recovery. They require less TFA in
the mobile phase for good peak shape, which increases
microbore sensitivity of peptides and proteins by
reducing quenching. Vydac MS columns are also
applicable to hydrophobic proteins and peptide mapping.
Differentiated Phases: C18, EPS C18
Differentiated Phases: C18, C8, C4

proprietary endcapping, 100 pore size
Specifications: Spherical silica, polymerically and

monomerically bonded, endcapped, 300 pore size
Formats: Capillary, Microbore, Expedite, Rocket,

Solvent-Reducer, Analytical, Prep
GraceSmart Columns
Vydac ProZap Columns
Classic reversed-phase and premium

performance at an exceptional value
High-speed, high-efficiency columns

for fast protein analysis
GraceSmart HPLC phases use high purity silica.

Which translates into symmetrical peaks for acids and
bases, and predictable reversed-phase selectivity.
Whether routine analysis or new method development,
use individually tested GraceSmart columns to get
premium performance at exceptional value.
Short 10 and 20mm ProZap columns with 1.5m

particles provide high-speed protein and life science
separations. Sharp, efficient peaks maximize method
sensitivity.
Differentiated Phases: C18
Specifications: Sub 2m spherical silica, proprietary

bonding and endcapping, 500 pore size

Column Families Suitable for Small Molecules

(<2000 Molecular Weight) (continued)
Format: Expedite
Format: Analytical
GraceAlpha Columns
A New Silica Generation
New silica from Grace with high porosity surface
and dense core ideal for scale-up applications.
While high porosity surface increases mass transfer
results in increased column efficiencies and loading
capacity, dense core and highly spherical shape yields
mechanically robust particle.
Differentiated Phases: C18, C8, and Silica
Specifications: High purity spherical silica with high
porosity surface and dense core
Formats: Analytical, Prep
Vydac Everest Columns

For peptide separations, peptide
mapping and proteomics applications
High-capacity Everest C18 columns are recommended
as the first choice in peptide separations. Everest
offer high capacity to resolve complex samples such as
protein digests.
Formats: Capillary, Microbore, Expedite, Analytical
25
analytical hplc
HPLC Column Selection
A Comparison of Reversed-Phase Columns
26
Based on the widely accepted work and data of Drs. Lloyd Snyder and John Dolan.1,2 Grace has developed this column selection
tool for choosing reversed phase HPLC columns based on peak capacities and column selectivity of polar and nonpolar compounds.
Typically, chromatographers choose HPLC columns by comparing physical characteristics, such as surface area and carbon load.
Often, this does not provide enough information about selectivity or capacity for adequate column selection. This chart provides a
reliable means of choosing HPLC columns based on acidic, basic, and hydrophobic character.
The Snyder/Dolan column test procedure has been described in a series of publications. Based on retention data for a series of
standard mixtures and the same separation conditions (50% acetonitrile/buffer; pH 2.8 and 7.0; 35C), reversed-phase columns
are characterized by five column-selectivity parameters: hydrophobicity (H), steric interaction (S*), hydrogen-bond acidity A ,
basicity (B), and relative silanol ionization or cation-exchange capacity C . Here we have chosen to graphically highlight data
for H , A , and C , with C results at pH 7.0. Hydrophobicity (H)
is often the primary analyte interaction with reversed phase
columns and indicates overall capacity. Secondary interactions are often polar interactions with basic analytes. The degree of
unprotonated base interaction A and protonated base interaction C with the packing material is measured and represented here.
Key to Chart

Hydrophobic Indicator

A Hydrogen bonding
IndicatorpH 2.8
(Protonated under
acidic conditions)
C Cation Exchange

IndicatorpH 7.0
(Unprotonated under
neutral conditions)
Directions for Using the Column Chart

. To find similar HPLC columns to test as
The chart lists the columns in descending order of hydrophobic capacity (H)
back-up columns, follow these steps.
1) Find the column you are currently using and note neighboring columns which have similar (H) capacity factors.
2) Compare the values for interaction of polar compounds ( A and C ).
If there is more than one choice for a back-up column, then compare your actual sample to the test probes. If your
sample is nonpolar, then place more emphasis on hydrophobic values. If your sample is basic (polar), then pay special
attention to A and C and determine if your sample will be protonated ( A ) or unprotonated ( C ) and place greater
emphasis on one of these values.
Manufacturer
Column
Selectivity Parameters
ZirChrom
ZirChrom-PBD C18
YMC
JSphere H80 C18
Restek
Allure C18
Phenomenex
Ultracarb ODS (30)
YMC Pack Pro C18 RS
Thermo/Hypersil
Hypersil BetamaxNeutral C18
Agilent
Zorbax Extend C18
Agilent
Zorbax C18
Beckman
Ultrasphere ODS
Alltima HP C18 High Load
Agilent
Zorbax Eclipse XDB-C18
Supelco
Ascentis C18
Macherey Nagel
Nucleodur C18 Gravity
Grace (Grom)
Grom Sapphire 110 C18
Restek
Restek Ultra C18
Varian
OmniSpher 5 C18
Grace (Vydac)
Denali 120 C18
Waters
Symmetry C18
Akzo Nobel
Kromasil 100-5C18
Waters
Nova-Pak C18
Thermo/Hypersil
Hypersil 100 C18
MacMod/ACT
ACE 5 C18-HL
ZirChrom
ZirChrom-EZ C18
Grace (Grom)
Grom Sil 120 ODS-5 ST
Dionex
Acclaim 120 C18
Waters
Sunfire C18
Agilent
Zorbax Eclipse Plus C18
Merck
Superspher 100 RP-18e
Shiseido
CAPCELL C18 AG120
Grace (Grom)
Grom Sil 120 ODS-3 CP
Waters
Delta-Pak C18 100A
Macherey Nagel
Nucleodur Isis
Phenomenex
Prodigy ODS (3)
Phenomenex
Synergi Hydro-RP C18
Phenomenex
Luna C18
Supelco
Supelcosil LC-18
YMC
YMC Pro C18
Phenomenex
Onyx Monolithic C18
Bischoff
ProntoSIL SpheriBOND 80-5-ODS2
Grace (Jones)
Apex II C18
Shiseido
CAPCELL C18 UG120
GL Sciences
Inertsil ODS-2
1.132
Bischoff
ProntoSIL 120-5-C18 H
1.116
Shiseido
CAPCELL C18 M G
1.114
Grace (Jones)
Genesis 120 C18
1.114
Bischoff
EU Reference Column C18
1.103
Grace (Alltech)
Allsphere ODS2
1.098
Merck
Purospher STAR RP18e
1.098
Merck
Chromolith RP18e
1.089
Phenomenex
Luna C18(2)
1.085
Varian
Pursuit C18
A
C
Adsorbosphere UHS C18
Zorbax Rx-18
Selectivity Parameters
LiChrospher 100 RP-18
YMC
Grace (Alltech)
Column
Merck
1.284
Grace (Alltech)
Agilent
Manufacturer
A
A
C A
C
Zorbax StableBond 80A C18
Phenomenex
Prodigy ODS(2)
1.056
Thermo/Hypersil
Hypersil BDS C18
1.055
Grace (Alltech)
Alltima C18
1.055
Grace (Vydac)
Vydac Everest C18
1.055
Thermo/Hypersil
Hypersil Beta Basic-18
1.052
GL Sciences
Inertsil ODS-3
1.052
Grace (Alltech)
Adsorbosphere C18
1.051
Phenomenex
Synergi Max-RP C18
1.049
Shiseido
CAPCELL C18 SG120
A
A
C
A
A
C
A
C
C
A
C
Alltima HP C18
0.993
C
C
C
C
0.993
0.993
A
A
0.993
0.990
A
0.989
0.989
0.987
1.035
Waters
Xterra MS C18
Waters
Symmetry 300 C18
1.031
Supelco
Discovery C18
1.030
Supelco
Supelcosil LC-18-DB
0.985
0.985
0.985
1.032
C
A
0.984
0.984
0.984
A
C
0.979
1.029
Thermo/Hypersil
Hypersil ODS
1.028
Bischoff
ProntoSIL 120-5-C18-AQ
0.974
1.023
Grace (Jones)
Genesis 300 C18 C18
0.974
1.023
Bischoff
Prontosil 200-5-C18 AQ
1.022
Agilent
Zorbax C8
1.018
Tosoh
TSK gel ODS-80Ts
1.018
Waters
Resolve C18
1.015
Phenomenex
Gemini C18 110A
1.012
Grace (Alltech)
Econosil C18
1.010
Phenomenex
Aqua C18
1.008
YMC
YMC ODS-AQ C18
A C
A
C
A
C A
A
A
Grace (Alltech)
0.995
Spherisorb S5 ODSB
C A
1.040
0.996
C A
Hypersil Bio Basic-18
0.998
Waters
Hypersil ODS-2
1.000
Thermo/Hypersil
C
C
Apex I C18
Thermo/Hypersil
1.030
Grace (Jones)
1.030
1.048
1.045
A
C
1.002
1.001
C A
Agilent
1.003
1.003
1.077
1.004
1.077
1.004
ACE 5 C18
A
CA
TSKgel Super-ODS
1.005
1.005
MacMod/ACT
Tosoh
1.005
A
1.080
1.006
1.077
C
C
1.007
Waters
Spherisorb ODS-2
1.007
Macherey Nagel
Nucleosil 100-5-C18 HD C18
0.975
AC
C
0.974
AC
0.974
0.974
0.974
C A
0.971
C A
C
0.968
0.967
0.966
0.966
0.965
AC
0.962
0.961
analytical hplc
Grace (Jones)
Genesis 120 AQ C18
C A
0.960
Macherey Nagel
Nucleodur Sphinx RP
Macherey Nagel
Nucleodur Pyramid
0.958
Thermo/Hypersil
Aquasil C18
Thermo/Hypersil
Hypersil Elite C18
0.958
MacMod/ACT
ACE AQ EP
Acclaim 300 C18
C A
0.957
Waters
Xterra MS C8
C A
Bischoff
ProntoSIL 300-5-C18 H
0.956
Phenomenex
Luna C5
Delta-Pak C18 300A
ProntoSIL HyperSORB 120 ODS
Thermo/Hypersil
Hypersil PAH C18
Bischoff
ProntoSIL 120-5-C18 Aqplus
Phenomenex
Jupiter 300 C18
CA
Varian
Polaris C18-Ether
Waters
Atlantis T3 C18
Tosoh
TSKgel 80Ts QA
Grace (Alltech)
Alltima C18-WP
Waters
YMC Hydrosphere C18
Grace (Alltech)
Brava BDS C18
Bischoff
ProntoSIL 60-5 C8 SH
0.955
Waters
MicroBondapak C18
Agilent
CA
AC
0.949
Agilent
Zorbax Rx-C8
CA
0.947
Grace (Alltech)
Platinum C18
CA
AC
C A
CA
Varian
Polaris C18-A
C A
YMC
JSphere M80 C18
Agilent
Zorbax Eclipse XDB-C8
Waters
Atlantis dC18 b
Supelco
Ascentis Express C8
Thermo
Hypersil GOLD aQ
Phenomenex
Selectosil C18
Merck
LiChrosorb RP-18
Grace (Vydac)
Vydac 218TP C18
A
A
AC
0.941
Grace (Alltech)
Alltima C18-LL
C A
0.940
Bischoff
ProntoSIL 120-5-C18 ace-EPS
Vydac 218MS C18
0.938
Waters
Spherisorb C8
0.929
YMC
JSphere L80 C18
0.928
Bischoff
ProntoSIL 300-55-C18 ace-EPS
0.926
Dionex
Acclaim Organic Acid C18
0.919
Waters
Xterra C18 RP
0.917
Grace (Alltech)
Alltima C8
0.915
Whatman
Partisil C8
CA
0.915
C A
0.911
C A
C
0.786
VisionHT C18
Luna Phenyl-Hexyl
Acquity UPLC BEH phenyl
C A
A
C
Grace
Grace (Vydac)
Merck
LiChrospher 60 RP-Select B C18
Bischoff
ProntoSIL 120-5 C8 SH
0.909
Grace (Alltech)
Allsphere ODS1
0.909
Waters
Symmetry Shield C8
0.792
0.786
Phenomenex
Waters
0.795
CA
0.945
0.938
0.798
AC
0.943
0.937
A
A
0.803
0.800
C A
0.951
AC
0.804
0.782
0.780
0.772
AC
0.770
AC
0.764
CA
0.763
AC
0.762
CA
0.762
A C
0.761
C
A
0.757
CA
0.756
CA
0.749
CA
0.747
AC
0.739
C A
C
0.733
0.730
AC
Waters
Acquity UPLC BEH Shield RP18 EP
0.907
Thermo/Hypersil
Hypurity C4
CA
0.713
Macherey Nagel
Nucleosil C18
CA
0.906
MacMod/ACT
ACE 5 C4-300
CA
0.710
Agilent
C A
C
A
Grace (Alltech)
Prosphere C18 300
Grace (Vydac)
Vydac 201TP C18
Supelco
Ascentis C-8
Waters
Nova-Pak C8
Waters
Symmetry C8
YMC
YMC Pro C8
Agilent
Zorbax Eclipse Plus C8
Phenomenex
Luna C8(2)
Grace (Alltech)
Prevail C18
C A
0.888
Grace (Alltech)
Prosphere 100 C18
C
A
0.883
Grace (Alltech)
Alltima AQ EP
Thermo/Hypersil
Hypersil GOLD C18
Phenomenex
Synergi Fusion-RP EP
Phenomenex
Luna C8
Grace (Grom)
Grom Sil 120 Octyl-6 MB C8
Grace (Jones)
Apex I C8
Shiseido
CAPCELL C18 A Q
Macherey Nagel
Nucelosil 100-5-C8 HD
Akzo Nobel
Kromasil 100-5C8
Grace (Jones)
Genesis 120 EC C8
Grace (Alltech)
Prevail Amide EP
Macherey Nagel
Nucleosil ODS
Restek
Ultra AQ C18
Waters
Sunfire C8
Waters
Acquity UPLC BEH C8
Shiseido
CAPCELL PAK C8 UG120
Waters
Symmetry Shield C18
Grace (Alltech)
Alphabond C18
Supelco
Ascentis RP-Amide
0.905
Varian
Polaris C8-Ether
0.903
Bischoff
ProntoSIL 60-5-Phenyl
0.901
Waters
Nova-Pak Phenyl
0.899
Macherey Nagel
Nucleosil 100-5-C18 Nautilus
CA
0.899
Agilent
C A
0.893
Bischoff
ProntoSIL SpheriBOND 80-5-ODS1
0.890
Thermo/Hypersil
Fluophase RP F
AC
C
C
C
C
Phenomenex
Jupiter 300 C4
0.889
Grace (Alltech)
Prosphere 300 C4
Bischoff
Prontosil 60-5-C4
Waters
Xterra Phenyl
0.882
Waters
Spherisorb ODS-1
0.881
Thermo/Hypersil
Hypersil Prism C18 RPN
0.879
Thermo/Hypersil
Fluophase PFP F
0.875
Agilent
Zorbax XDB-Phenyl
0.872
Waters
Symmetry 300 C4
0.869
Waters
Xterra C8 RP
0.867
Grace (Alltech)
Alltima HP C18 EPS
C A
0.865
Supelco
Discovery BIO Wide pore C5
0.864
Agilent
Zorbax Bonus RP EP
0.863
Phenomenex
Synergi Polar-RP C18
A
C

0.862
MacMod/ACT
ACE Phenyl
C A
C
C
A
C
C A
C A
CA
0.860
Grace (Jones)
Genesis 120 C4 EC
0.857
Thermo/Hypersil
Hypersil Prism C18 RP
0.856
Thermo/Hypersil
BetaMax Acid EP
C A
0.855
Supelco
Discovery HS F5 F
0.854
Agilent
Zorbax SB-Phenyl
0.850
Grace (Alltech)
Platinum EPS C18
A
C
C
C
A
C
A
CA
0.705
A
0.702
A C
0.698
0.683
0.659
AC
0.657
C
0.654
0.654
0.646
AC
C
0.645
0.635
CA
A
0.841
Grace (Jones)
Genesis 300 C4 C4
AC
0.839
Grace (Jones)
Genesis Phenyl
A C
0.835
Agilent
0.834
MacMod/ACT
ACE Phenyl-300
0.834
Agilent
Zorbax SB-AQ EP
Thermo/Hypersil
Hypersil Beta Basic-8
Grace (Alltech)
Alltima HP C8
Thermo/Hypersil
Hypurity C8
C A
0.833
ZirChrom
ZirChrom-PS EP
Supelco
Discovery C8
C A
0.832
Waters
MicroBondapak Phenyl
A
CA
0.830
Grace (Alltech)
Platinum C8
0.830
Grace (Alltech)
Platinum EPS C8 300
Grace (Jones)
Genesis 120 C8
0.829
Thermo/Hypersil
BetaBasic Phenyl
Thermo
Hypersil GOLD C8
0.825
Macherey Nagel
Nucleosil C8
Phenomenex
Onyx Monolithic C8
0.824
Macherey Nagel
EC Nucleosil 100-5 Protect 1 EP
Tosoh
TSKgel Super-Octyl
C A
0.824
Bischoff
Prontosil 120-5-C8 ace-EPS
Phenomenex
Prodigy Phenyl-3
YMC
YMC Basic C18
C A
0.821
Grace (Alltech)
Alltima HP C18 Amide
Grace (Alltech)
Econosphere C18
0.818
Thermo/Hypersil
BetaMax Base EP
Tosoh
TSKgel Octyl-80Ts
Whatman
Partisil ODS(3)
A
C
AC
0.814
Grace (Alltech)
Platinum EPS C8
0.810
Thermo/Hypersil
Hypurity Advance
0.599
0.593
Inertsil C8-3 C8
Agilent
0.609
0.601
C
A
ACE 5 C8
0.821
0.615
AC
GL Sciences
0.822
0.617
AC
MacMod/ACT
AC
0.619
AC
AC
CA
0.619
AC
VisionHT C18-P
0.623
C
A
Prevail C8
0.631
0.647
AC
Grace
Prevail Select C18
0.654
A C
Grace (Alltech)
Hypersil Bio Basic-8
0.655
CA
0.843
Grace (Alltech)
0.665
AC
0.845
Thermo/Hypersil
0.675
AC
Grom Sapphire 110 C8
0.678
Grace (Grom)
C A
0.682
C A
CA
CA
0.686
C
A
CA
0.689
CA
0.698
AC
Purospher RP-18
C A
0.700
AC
Discovery BIO Wide pore C8
0.701
CA
Merck
0.704
A C
Supelco
C A
0.705
0.889
Waters
Bischoff
0.805
C A
Dionex
0.805
AC
0.589
0.585
0.584
0.584
AC
A
0.582
0.575
AC
C
0.544
0.532
A C
0.529
0.526
CA
C
A
A
C
AC
AC
0.497
0.470
0.420
0.412
References:
1. The Hydrophobic-subtraction Model of Reversed-phase Column Selectivity, L.R. Snyder, J.W. Dolan and P.W. Carr, J. Chromatogr. A, 1060 (2004) 77-116.
2. A New Look at the Selectivity of Reversed-phase HPLC Columns, L.R. Snyder, J.W. Dolan and P.W. Carr, Anal. Chem., 79 (2007) 3255-3262.
27
analytical hplc
Grace HPLC Packing Material Specifications

Columns for Small Molecules
Brand
Phase
Adsorbosil
C18
C8
C2
CN
NH2
Silica
Adsorbosphere C18
C18 HS
C18 UHS
C8
Phenyl
Cyano
Cyano-AQ
Amino (NH2)
Silica
SAX
SCX
Adsorbosphere XL C18
C18-B
C8
C1 (TMS)
Silica
SAX
SCX
Allsphere
ODS-1
ODS-2
C8
C6
C1 (TMS)
Phenyl
Cyano
Amino (NH2)
Silica
SAX
SCX
Alltima HP
C18
C18 EPS
C18 HiLoad
C18 AQ
C18 Amide
C8
Cyano
Silica
HILIC
Alltima
C18
C18 LL
C8
Phenyl
Cyano
Amino (NH2)
Silica
AlphaBond
C18
C8
Phenyl
Cyano
Amino (NH2)
Silica
Apex I
ODS
C8
C8(EC)
C1
Phe
Basic ODS
PAH
CN
Amino (NH2)
Carbohydrate
Silica
Apex II
ODS
Diol
Amino (NH2)
Othersas Apex I
Apex Prepsil
ODS
C8
C8(EC)
C2
CN
Amino (NH2)
Silica
Diol
Proprietary.
28
Base
Material
Particle
Shape
Particle Size
Pore Size
Surface Area
Carbon
Load
Phase Type
EndUSP
capped? L-code
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Irregular
Irregular
Irregular
Irregular
Irregular
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Irregular
Irregular
Irregular
Irregular
Irregular
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
5, 10m
5, 10m
5, 10m
5, 10m
5, 10m
5, 10m
3, 5, 10m
3, 5m
5, 10m
3, 5, 10m
5m
5m
5m
3, 5m
5m
5m
5m
3, 5m
5m
3, 5m
5m
5m
5, 10m
5, 10m
5m
3, 5m
3, 5m
5m
5m
5m
5m
5m
3, 5m
5m
5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
1.5, 3, 5m
3, 5, 10m
5m
3, 5, 10m
3, 5m
3, 5m
3, 5m
3, 5, 10m
5, 10m
10m
10m
10m
10m
10m
3, 5, 10m
3, 5m
3, 5m
3, 5m
3, 5m
5m
5m
3, 5m
3, 5m
5m
3, 5, 10m
3, 5m
3, 5m
3, 5m
5m
8, 15m
8, 15m
8, 15m
8m
8m
8m
8, 15m
8m
60
60
60
60
60
60
80
60
60
80
80
80
120
80
80
80
80
90
90
90
90
90
90
90
80
80
80
80
80
80
80
80
80
100
100
190
190
100
100
190
190
190
100
120
100
100
100
100
100
100
100
125
125
125
125
125
125
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
130
130
130
130
130
130
130
130
450m /g
450m2/g
450m2/g
450m2/g
450m2/g
450m2/g
200m2/g
350m2/g
500m2/g
200m2/g
200m2/g
200m2/g
170m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
220m2/g
220m2/g
220m2/g
220m2/g
220m2/g
220m2/g
220m2/g
220m2/g
220m2/g
220m2/g
220m2/g
200m2/g
200m2/g
450m2/g
450m2/g
200m2/g
200m2/g
200m2/g
450m2/g
230m2/g
340m2/g
340m2/g
340m2/g
340m2/g
340m2/g
340m2/g
340m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
200m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
15%
10%
No
12%
20%
30%
8%
5%
11%
12%
6%
7%
12%
6%
4%
3%
3%
3.5%
3%
4%
4%
12%
4%
24%
20%
12%
8%
4%
16%
9%
9%
7.5%
10%
10%
7%
7%
2.5%
3%
12%
4%
2%
10.5%
3.5%
2%
10%
7%
7%
2.8%
4%
2%
3.2%
Polymeric
Polymeric
Polymeric
Polymeric
Polymeric
Polymeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Polymeric
Polymeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Polymeric
Polymeric
Polymeric
Polymeric
Polymeric
Polymeric
Monomeric
Monomeric
Monomeric
Monomeric
Polymeric
Polymeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Polymeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Yes
Yes
No
Yes
No
No
Yes
Yes
Yes
Yes
Yes
Yes
No
No
No
No
Yes
Yes
Yes
Yes
Yes
No
Yes
Yes
Partial
Yes
Yes
Yes
No
Yes
No
No
No
No
No
Yes
No
Yes
Yes
Yes
Yes
Yes
No
No
Yes
Yes
Yes
Yes
Yes
No
No
Yes
Yes
Yes
Yes
No
No
Yes
No
Yes
Yes
No
Yes
Yes
No
No
No
Yes
No
No
No
Yes
No
Yes
No
Yes
Yes
No
No
L1
L7
L16
L10
L8
L3
L1
L1
L1
L7
L11
L10
L10
L8
L3
L1
L1
L7
L13
L3
L1
L1
L7
L15
L13
L11
L10
L8
L3
L1
L1
L1
L1
L1
L7
L10
L3
L3
L1
L1
L7
L11
L10
L8
L3
L1
L7
L11
L10
L8
L3
L1
L7
L7
L13
L11
L1
L10
L8
L3
L1
L8
L1
L7
L7
L30
L10
L8
L3
L20
analytical hplc
Columns for Small Molecules (continued)
Phase
Base
Material
Particle
Shape
Particle Size
Pore Size
Surface Area
Carbon
Load
Apollo
C18
C8
Phenyl
Silica
C18 BDS
C18 ODS
C8
C8 BDS
Phenyl
Cyano
Cyano BDS
Amino (NH2)
Silica
Amino
Cation
238DE C18
C18
C8
CN
NH2
Silica
C18
C8
Cyano
Amino (NH2)
Silica
C18
C18 AQ
C8
C8(EC)
C4
Phenyl
CN
Amino (NH2)
Carbohydrate
CN-TCA
Petro-XP
Silica
C18
C8
Silica
C18
ODS-0 AB (acid/base deactivated)
ODS-2 FE (fully endcapped)
ODS-3 CP (encapsulated)
ODS-4 HE (hydrophilic endcapping)
ODS-5 ST (standard)
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Polymer
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Irregular
Spherical
Spherical
Irregular
Irregular
Irregular
Irregular
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
5m
5m
5m
5m
3, 5m
3, 5m
3, 5m
3, 5m
5m
5m
5m
5m
5m
10m
10m
3, 5, 10, 15, 20m
3, 5, 10m
3, 5, 10m
5, 10m
5, 10m
3, 5, 10m
3, 5, 10m
3, 5, 10m
5m
5m
3, 5, 10m
3, 4, 7, 15m
4, 7m
3, 4, 7, 15m
3, 4, 7, 15m
4m
4m
3, 4m
3, 4m
4m
4m
4m
3, 4, 7, 15m
5, 10, 15, 20m
5, 10, 15, 20m
5, 10, 15, 20m
3, 5m
1.5, 3, 5, 10m
1.5, 3, 5, 10m
3, 5, 7, 10m
3, 4, 5, 7, 10m
3, 4, 5, 7, 10m
100
100
100
100
145
130
130
145
130
130
145
130
130
80
120
80
80
80
80
80
80
80
80
80
80
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
100
80, 100, 300
120, 300
120, 200
60, 120, 200, 300
ODS-6 NE (non endcapped)

ODS-7 pH (pH-stable)
Octyl-1 B (base deactivated)
Octyl-2 AB (acid/base deactivated)
Octyl-3 BA (for bases)
Octyl-4 FE (fully endcapped)
Octyl-5 CP (encapsulated)
Octyl-6 MB (monomer binding)
Hexyl-1 MB (monomeric bonding)
Phenyl-1 FE (fully endcapped)
Phenyl-2 CP (encapsulated)
Phenyl-3 PE (partially endcapped)
Butyl-1 ST (standard)
Butyl-2 FE (fully endcapped)
TMS-1 ST (standard)
TMS-2 CP (encapsulated)
Cyan-1 ST (standard)
Cyan-2 PR (cyanopropyl)
Cyan-3 CP (encapsulated)
Amino-1 PR (NH-propyl)
Amino-2 PA (cross linked Poly-NH2)
Amino-3 CP (encapsulated NH-residues)
Amino-4 PR (propylamine bonded
to silica)
Diol
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Irregular
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Irregular
Irregular
3, 5m
4m
3, 5m
3, 5m
3, 5m
3, 5, 10m
3, 5, 7, 10m
3, 5, 10m
5m
3, 5, 10m
5m
3, 5, 10m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
5m
3, 5, 10m
5m
5m
3, 7m
120
80
100
100
120
80, 100, 300
120, 300
120, 200, 300
80, 100
120, 300
120, 300
80, 100
120, 300
300
120, 300
120, 300
120, 300
80, 100
120
80, 100
120
80
300
340m2/g
340m2/g
340m2/g
340m2/g
185m2/g
195m2/g
195m2/g
185m2/g
195m2/g
195m2/g
185m2/g
195m2/g
195m2/g
550m2/g
280-340m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
325m2/g
325m2/g
325m2/g
220m2/g
200m2/g
220, 200, 100m2/g
320, 170m2/g
300, 200m2/g
580, 300, 200,
150m2/g
300m2/g
510m2/g
200m2/g
200m2/g
300m2/g
220, 200, 100m2/g
320, 170m2/g
300, 200, 150m2/g
220, 200m2/g
300, 150m2/g
320, 170m2/g
220, 200m2/g
300, 150m2/g
100m2/g
300, 150m2/g
320, 170m2/g
300, 150m2/g
220, 200m2/g
320m2/g
220, 200m2/g
300m2/g
420m2/g
100m2/g
15%
Monomeric
9%
Monomeric
8%
Monomeric
8.5%
Monomeric
8.5%
Monomeric
6%
Monomeric
5.5%
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Polymeric
20%
Monomeric
10%
Monomeric
5%
Monomeric
Monomeric
Polymeric
10%
Monomeric
5%
Monomeric
Monomeric
Polymeric
18%
Monomeric
15%
Monomeric
11%
Monomeric
11%
Monomeric
6%
Monomeric
9%
Monomeric
7%
Monomeric
3.5%
Polymeric
Monomeric
7%
Monomeric
Monomeric
15%
Monomeric
10%
Monomeric
10%
Monomeric
11%
Monomeric
12, 11, 6% Monomeric
15, 6%
Polymeric
16, 11% Monomeric
22, 17,
Monomeric
12, 7%
17%
Monomeric
22%
Polymeric
6.5%
Monomeric
5%
Monomeric
9%
Monomeric
6.6, 6, 3% Monomeric
10, 5.5% Polymeric
10, 7, 4% Monomeric
4, 4%
Monomeric
9, 5%
Monomeric
7, 4%
Polymeric
6.6, 6% Monomeric
7, 2.5% Monomeric
1, 5%
Monomeric
4%
Monomeric
3%
Polymeric
4.8%
Monomeric
3.5%
Monomeric
4%
Polymeric
2%
Monomeric
Polymeric
Monomeric
Monomeric
Silica
Spherical
5, 10m
Normal Phase-1 ST (standard silica)

Normal Phase-2 SP (spherical silica)
Silica
Silica
Spherical
Spherical
3, 5, 10m
3, 5, 10m
Normal Phase-3 PV (polyvinylalcohol)

SEC (size exclusion chromatography)
Silica
Silica
Spherical
Spherical
5m
5, 10m
Strong Anion-1
Weak Anion-2 (ion exchange)
Strong Cation-1 (ion exchange)
Silica
Silica
Silica
Spherical
Spherical
Spherical
5, 10m
7m
5, 10m
60, 120, 200, 300 580, 300, 200,

150m2/g
80, 100, 1000
220, 200m2/g
60, 120, 200,
580, 300, 200m2/g
1000
120
300m2/g
60, 120, 200, 300 580, 300, 200,
150m2/g
80, 100
220, 200m2/g
300
100m2/g
80, 100
220, 200m2/g
Brava
Carbohydrate
Denali
Econosil*
Econosphere*
Genesis 120
Grace Alpha
GraceSmart
Grom Sil
*Available only online.
Phase Type
EndUSP
capped? L-code
Yes
Yes
Yes
No
Yes
Yes
Yes
Yes
No
No
No
No
No
No
No
Yes
Yes
Yes
Yes
No
No
Yes
Yes
Yes
No
No
Yes
Yes
No
Yes
Yes
Yes
Yes
No
No
Yes
No
No
Yes
No
No
Yes
Yes
Yes
No
Yes
Yes
L1
L7
L11
L3
L1
L1
L7
L7
L11
L10
L10
L8
L3
L1
L1
L7
L10
L8
L3
L1
L7
L10
L8
L3
L1
L1
L7
L7
L26
L11
L10
L8
L3
L1
L7
L3
L1
L1
L1
L1
L1
L1
No
No
Yes
Yes
Yes
Yes
No
Yes
Yes
Yes
No
Yes
No
No
Yes
No
Yes
Yes
No
Yes
No
Yes
No
L1
L1
L7
L7
L7
L7
L7
L7
Brand
L11
L11
L11
L26
L26
L13
L13
L8
L8
L8
L8
Monomeric No
L20
No
No
L3
L3
Polymeric
No
No
L3
No
No
No
29
analytical hplc
Columns for Small Molecules (continued)
Brand
Phase
Base
Material
Particle
Shape
Particle Size
Pore Size
Surface Area
Carbon
Load
Phase Type
EndUSP
capped? L-code
Grom Sil (cont.)
Weak Cation-2 (ion exchange)

HIC (hydrophobic interaction chrom.)
C18
C8
C4
Silica
C18/Cation
C8/Anion
C8/Cation
C18
C18 EPS
C8
C8 EPS
Phenyl
Cyano
Amino (NH2)
Silica
SAX
C18 Select
C18
C8
Phenyl
Cyano
Amino (NH2)
Silica
Organic Acid
Carbohydrate ES
101SP Sil
201SP C18
208SP C8
C18
C-18-B
C18-P
C18-HL
HILIC, Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Polymer
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spheroidal
Spheroidal
Spheroidal
Spherical
Spherical
Spherical
Spherical
Spherical
7m
7m
3, 5, 10m
3, 5, 10m
3, 5, 10m
3, 5, 10m
5, 7m
7m
5m
1.5, 3, 5m
1.5, 3, 5m
1.5, 3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
5m
5, 10m
3, 5, 10, 15m
5, 10, 15m
1.5m
1.5m
1.5m
1.5m
1.5m
300
300
65, 110
65, 110
65, 110
65, 110
100
100
100
100
100
100
100
100
100
100
100
100
110
110
110
110
110
110
110
110
100
90
90
100
120
100
120
120
100m2/g
100m2/g
500, 270m2/g
500, 270m2/g
500, 270m2/g
500, 270m2/g
350m2/g
350m2/g
350m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
350m2/g
350m2/g
350m2/g
350m2/g
350m2/g
350m2/g
350m2/g
350m2/g
250350m2/g
250350m2/g
250350m2/g
200m2/g
220m2/g
200m2/g
220m2/g
220m2/g
23, 16%
15, 10%
10.5, 7%
6%
5%
4%
2.5%
17%
15%
8%
7%
13%
9%
6%
5.5%
5%
11%
Monomeric
Monomeric
Monomeric
Polymeric
Polymeric
Polymeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
unbonded
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Polymeric
No
No
Yes
Yes
Yes
No
No
No
No
Yes
No
Yes
No
Yes
No
No
No
No
Yes
Yes
Yes
Yes
Yes
No
No
Yes
No
No
Yes
Yes
Yes
No
No
Grom Sapphire
Mixed Mode
Platinum
Prevail
Vydac SP
VisionHT
L1
L7
L26
L3
L1
L1
L7
L7
L11
L10
L8
L3
L1
L1
L7
L11
L10
L8
L3
L3
L1
L7
L1
L1
L1
L1
L3
Columns for Large Molecules

Brand
Base
Material
Particle
Shape
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Polymer
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spheroidal
Spheroidal
Spheroidal
202TP C18
208TP C8
Silica
Silica
Spheroidal
Spheroidal
214TP C4
Silica
Spheroidal
218TP C18
Silica
Spheroidal
219TP Di-Phe
Silica
Spheroidal
238TP C18
Silica
Spheroidal
238EV C18
208MS C8
214MS C4
218MS C18
238MS C18
219MS Di-Phe
Silica
Silica
Silica
Silica
Silica
Silica
Spherical
Spheroidal
Spheroidal
Spheroidal
Spheroidal
Spheroidal
Phase
Genesis 300
C18
C4
CN
Macrosphere 300 C18
C8
C4
SAX
WAX
SCX
WCX
Macrosphere GPC GPC 60
GPC 100
GPC 150
GPC 300
ProSphere
C18
C18-AQ
C4
Size-Exclusion 125
Size-Exclusion 250
Size-Exclusion 450
P-HR (reversed phase)
ProZap
C18 ProZap
Vydac ATP
214ATP C4
Vydac TP
101TP Sil
201TP C18
Everest
Vydac MS
*Product information is available at www.discoverysciences.com. Proprietary.
30
Particle Size
Pore Size
Surface Area
Carbon
Load
Phase Type
EndUSP
capped? L-code
4, 7m
4, 7m
4m
5, 7m
5, 7m
5, 7m
7m
7m
7m
7m
7m
7m
7m
7m
3, 5, 10m
3, 5m
3, 5, 10m
4, 5m
4, 5m
8m
4m
1.5m
5, 1015m
5, 10, 1015, 1520m
5, 7, 10, 1015,
1520m
3, 5, 10m
3, 5, 7, 10, 1015,
1520m
3, 5, 7, 10, 1015,
1520m
3, 5, 7, 10, 1015,
1520m
3, 5, 7, 10, 1015,
1520m
3, 5, 7, 10, 1015,
1520m
5, 10, 1015, 1520m
5m
5m
3, 5, 10, 1015m
5m
5m
300
300
300
300
300
300
300
300
300
300
60
100
150
300
300
100
300
125
250
450
140
500
300
300
300
120m2/g
120m2/g
120m2/g
100m2/g
100m2/g
100m2/g
100m2/g
100m2/g
100m2/g
100m2/g
450m2/g
350m2/g
200m2/g
100m2/g
120m2/g
450m2/g
120m2/g
59m2/g
70110m2/g
70110m2/g
7090m2/g
10%
3%
3.3%
10%
2.2%
9%
18%
3%
3%
3%
8%
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Polymeric
Polymeric
Polymeric
Polymeric
Polymeric
Polymeric
Monomeric
Monomeric
Monomeric
Monomeric
Polymeric
unbonded
Polymeric
Yes
Yes
Yes
Yes
Yes
Yes
No
No
No
No
No
No
No
No
Yes
Yes
Yes
No
No
No
No
Yes
Yes
No
No
L1
L26
L11
L1
L7
L26
L25
L1
L1
L26
L1
L26
L3
L1
300
300
6090m2/g
60110m2/g
9%
5%
Polymeric
Polymeric
No
Yes
L1
L7
300
60110m2/g
3%
Polymeric
Yes
L26
300
60110m2/g
8%
Polymeric
Yes
L1
300
60110m /g
4%
Polymeric
Yes
300
60110m /g
4%
Monomeric Yes
L1
300
300
300
300
300
300
70110m2/g
70m2/g
70110m2/g
60110m2/g
70m2/g
70m2/g
6%
5%
3%
8%
4%
4%
Monomeric
Polymeric
Polymeric
Polymeric
Monomeric
Polymeric
L1
L7
L26
L1
L1
Yes
Yes
Yes
Yes
Yes
Yes
analytical hplc
Grace HPLC Hardware Formats

Grace Davison Discovery Sciences provides hardware formats to maximize the performance of the phases in our column families
for all applications. Choose a hardware format based on your method requirements for speed, sensitivity, and resolution.
Ultra High-Pressure Hardware
Analytical Hardware
7135
7345
Ideally suited for ultra high-pressure systems, this ultralow volume hardware is pressure rated to 18,000psig,
and packed with 1.5m media to maximize speed and
efficiency.
Lengths
Inner
Diameters
20, 30, 50, 100mm 1.0, 2.0mm
Pressure
Limit
Wetted
Surfaces
18,000psig
316 Stainless Steel
4.6mm i.d. columns for standard HPLC instrumentation,

the most commonly used. Analytical columns have
industry standard port configurations. Some column
families are also available with port configurations for
Waters endfittings.
Lengths
30, 50, 100, 150,

250, 300mm
Expedite MS Hardware
10, 20mm
Inner
Diameters
Pressure
Limit
Wetted
Surfaces
2.1, 4.6mm
10,000psig
316 Stainless Steel
Rocket Hardware
33, 53mm
Pressure
Limit
Wetted
Surfaces
7mm
10,000psig
316 Stainless Steel
Solvent-Reducer and Microbore Hardware

7345
Smaller diameter columns reduce solvent consumption

and increase sensitivity when compared to standard
4.6mm i.d. columns. Use with standard HPLC
instrumentation or with MS and ELS detectors.
Lengths
4.6mm
10,000psig
316 Stainless Steel
Metal-Free Hardware
Mechanically strong, metal-free columns offer

biocompatibility, and chemical resistance that ion
chromatography and biotechnology applications demand.
6904
Inner
Diameters
Inner
Diameters
Wetted
Surfaces
7341
High-speed, high-resolution columns for high-throughput

analysis. Large 7mm i.d. balances column volume
with system volume to deliver excellent peak shapes
on conventional HPLC instrumentation. Large i.d. also
allows fast mobile-phase flow rates which minimizes
peak broadening.
Lengths
Pressure
Limit
6113
High-speed, low-volume columns for rapid resolution and

high-throughput LC/MS applications.
Lengths
Inner
Diameters
Pressure
Limit
50, 100, 150, 150, 1.0, 2.1, 3.0mm 10,000psig

250, 300mm
Lengths
Inner
Diameters
50, 100, 150, 250, 2.1, 4.6mm

300mm
Pressure
Limit
Wetted
Surfaces
5000psig
PEEK
Capillary Guards
6931
Capillary guards offer zero dead-volume with finger-tight

connections to maintain column performance. Use them
to protective your capillary investment, as enrichment
columns, or as short analytical columns.
Lengths
5, 10, 20mm
Inner
Diameters
Pressure
Limit
Wetted
Surfaces
0.3, 0.5mm
5000psig
316 Stainless Steel

and fused silica
All-Guard Guard Cartridges
Wetted
Surfaces
316 Stainless Steel
Capillary Hardware
6617
Guard system with reusable holder and disposable guard

columns to protect analytical columns.
Lengths
7.5mm
Inner
Diameters
Pressure
Limit
Wetted
Surfaces
2.1, 3.0, 4.6mm
5000psig
316 Stainless Steel
7344
For LC/MS and other high-sensitivity and sample-limited

applications.
Lengths
Inner
Diameters
Pressure
Limit
50, 100, 150, 250, 0.075, 0.150,

5000psig
300mm
0.3, 0.5, 0.8mm
Wetted
Surfaces
more info
Looking for preparative HPLC hardware?

See page 150.
316 Stainless Steel

and fused silica
31
analytical hplc
High Throughput HPLC

Introduction
System Type 1
Ultra High-Pressure LC System
The Benefits of Grace Small Particles and

Formats
Examples: Agilent 1200, Waters Acquity, Thermo Accela,

Jasco XLc.
Todays laboratories are under greater demand to analyze

more samples in less time. To help meet this demand, major
product advances have been made to decrease analysis
time and increase HPLC throughput. HPLC system advances
have greatly reduced the influence of extra column volumes
and have extended the range of flow rates and pressure
capabilities. In response to this, Grace now offers a wide
range of column chemistries and formats appropriate for
all types of high throughput systems.
The use of smaller particles offers two main improvements to
the chromatographic separationincreased resolution and
speed. Resolution is directly proportional to the square root
of column efficiency, therefore the higher the efficiency, the
narrower the peaks and the greater the resolution between
them. Increased speed comes from higher mobile phase flow
rates that can be used without loss in efficiency, and higher
flow rates mean faster analysis times.
Understanding the type of system currently in use for
high throughput separations and then matching the right
column configuration is critical to achieving the best high
throughput separation. Here we outline the three types of
systems currently in use today for high throughput and the
recommended HPLC column format for use with that system.
(>10,000psig pressure limitation)
Speed from Ultra High-Pressure Systems:

Representing the latest in LC instrumentation technology,
ultra high-pressure systems theoretically have the potential
to deliver the fastest separations. They have minimal system
volume and offer a pressure limit upwards of 12,000psig.
This allows the use of columns with sub 2m particles and
27 times traditional flow rates. Sub 2m particles extend the
working range of acceptable mobile phase linear velocities
without sacrificing efficiency. Therefore, you can push flow
rates and still get equal or better performance.
Suggested Column Format:

VisionHT columns are designed for microbore and ultra
high-pressure LC systems that have small system volumes
to limit peak broadening from sample diffusion. VisionHT
columns are packed with highly efficient 1.5m phases
that yield taller peaks and increase sensitivity. The column
hardware incorporates a low dead volume design to minimize
sample diffusion, and maintain peak integrity and efficiency.
12,000psig high-pressure stability allows fast flow rates,
decreasing run times 10 fold.
System Type 1
Recommended Column Format:
Length:
i.d.:
VisionHT
20, 30, 50, 100mm
1.0 and 2.0mm
tech tip
Converting Methods From Traditional Column

Formats to High Throughput Columns
7135
Convert Between Standard HPLC and VisionHT Columns
VisionHT Columns for

Ultra High-Pressure LC
Flow Rate
1.0X
2.3X
When adjusting between standard LC conditions to VisionHT

columns convert flow rates accordingly and then increase flow
rate for faster analysis.
Convert Between Standard HPLC Columns and Rocket Columns
Flow Rate
Standard Analytical (4.6mm)

Rocket Column (7.0mm)
1.0X
2.3X
Use this conversion of flow rate to transfer methods between

Rocket column or VisionHT columns. Backpressure on
standard LC systems should be considered.
20
1.
2.
3.
4.
5.
6.
7.
8.
15
mAU
Standard HPLC (4.6mm)

VisionHT (2.0mm)
10
2 56
7
1 34
0
0
System
Mobile Phase:

Detector:
Temperature:
more info
10724
Sulfadiazine
Sulfathiazole
Sulfamerazine
Sulfamethazine
Sulfamethoxazole
Sulfaisoxazole
Sulfaquinoxilone
Sulfamethoxine
5 Min.
Agilent 1200
A: 0.1% Formic Acid
B: Methanol
UV at 280nm
Ambient
See pages 3437 for more information on VisionHT columns.
32
analytical hplc
System Type 2
Low Volume, High Throughput (HTP)
LC System
(<10,000psig Pressure Limitation)
Speed from Low-Volume HTP LC Systems:
(<5000psi Pressure Limitation)

Examples: Agilent 1100, Waters Alliance, Thermo Surveyor,
Dionex Ultimate, Shimadzu Prominence, Hitachi LaChrom
Speed from Traditional LC Systems:
These systems concentrate on reducing the cycle time

between injections thus allowing more injections per time
frame. Typically they employ high-speed gradient pumps,
fast autosamplers and a quick detector sampling rates. They
are not pressure rated to the extremes of ultra high-pressure
systems, but the low system volume allows for short columns
of narrow i.d.
To get speed from a traditional LC system, you need to

consider the 5000psig pressure limitation, and the typically
large 2mL system volume. High throughput columns on this
system need to deliver a highly efficient separation typically
achieved with a small particle packing (3m), but without the
high backpressures. The large system volume also needs
to be balanced with an equally large column volume or the
separation will be plagued with extra-column effects.
Expedite and VisionHT columns are designed for

microbore high throughput LC systems that have small
system volumes. These formats incorporate a low dead
volume design so that sample bands do not diffuse within the
column hardware, maintaining peak integrity and efficiency.
Expedite columns are packed with highly efficient 1.5m or
3m in very short column lengths to minimize backpressure
and reduce analysis times. Though not as fast as ultra highpressure LC systems these systems and columns balance
both speed and backpressure.
Rocket columns provide low backpressure and fast analysis

times while preserving column efficiency. They are available
with both 1.5m and 3m packing materials for use on
standard HPLC systems with backpressure limits less than
5000psig. The 7mm i.d. allows faster flow rate that sweep
the extra system volume faster and reduce peak broadening.
This larger diameter also means a larger column volume to
system volume ratio to minimize the efficiency loss from extra
system volume. This benefit is more pronounced over 2.1
and 1mm i.d. columns that have a smaller ratio than 4.6mm
i.d. columns and require much lower flow rates for acceptable
backpressures. Low flow rates allow more time for sample
diffusion within the standard HPLCs system volume to further
degrade the columns efficiency.
System Type 2
Recommended Column Formats:
Length:
i.d.:
Expedite and VisionHT

10, 20, 30, 50mm
1, 2, 4.6mm
6113
Expedite Columns for Low Volume

High Throughput LC
System Type 3
Recommended Column Format:
Length:
i.d.:
Packings:
6904
Rocket Columns for High Throughput

with Conventional LC
7
10736
Rocket
33 or 53mm
7mm
Any 1.5m or 3m media
1.
2.
3.
4.
5.
6.
7.
Ribonuclease (37ng)
Bovine Insulin (37ng)
Cytochrome C (37ng)
Lysozyme (4ng)
Bovine Serum Albumin (37ng)
Carbonic Anhydrase (37ng)
Ovalbumin (37ng)
8679
1.
2.
3.
4.
5.
2
3
0
Column:
Mobile Phase:

Flow Rate:
Detector:
Injection Vol.:
more info
Examples: Shimadzu Prominence UFLC, Hitachi Ultra,

LC Packings UltiMate, Surveyor Plus
System Type 3
Traditional LC System
2 Min.
ProZap C18, 1.5m, 2.1 x 10mm Expedite

A: 0.1% TFA in Water
B: 0.085% TFA in Acetonitrile
0.8mL/min
UV at 280nm
5L
Expedite columns are available in most popular Alltech brand

phases. See page 24 25 for overview of phases and formats
offered.
0
Column:
Mobile Phase:
Flow Rate:
Detector:
Xanthine
Theobromine
Theophylline
Caffeine
8-Chlorotheophylline
2 Min.
Platinum C18-EPS 100, 3m, 7 x 33mm Rocket

0.010M Sodium Acetate, pH 4.0:Methanol (70:30)
5.0mL/min
UV at 254nm
more info
Rocket columns are available in most popular Alltech brand

phases. See page 24 25 for overview of phases and formats
offered.
33
analytical hplc
Grace VisionHT Ultra High-Pressure Columns
hplc columns | small molecule
See Separations with Speed and Clarity

Ultra-fast separations with superior efficiency, sensitivity, and resolution
Exceptional stability for long column lifetimes
Comprehensive sub 2m stationary phase offering
12,000psig pressure rating compatible with all ultra high-pressure LC systems
Grace VisionHT columns offer a new level of performance in HPLC. The powerful combination of
high-strength 1.5m media with ultra-low volume hardware, delivers new clarity to your separations
and maintains exceptional column lifetime. Complex samples resolve 95% faster with 4x greater
sensitivity when compared to traditional 2.1 x 150mm, 5m columns. And with a wide variety of
phases available, the possibilities are endless.
Increase Speed by 95% and Gain 4x Better Sensitivity

2500 mAU
2000
2500 mAU
2000
1500
1000
2500 mAU
500
2000
1500
13
4
1000
10
12
500
Thiourea
Toluene
Propylbenzene
Butylbenzene
0
0.5
14 Min.
System:
Mobile Phase:

Detector:
Temperature:
Column: Traditional C18, 5m, 2.1 x 150mm

2 Flow Rate: 0.2mL/min
3
4
500
Impurities
not seen on
traditional
5m columns.
0
0
1500
1000
Column: VisionHT C18, 1.5m,

2.0 x 50mm
Flow Rate: 0.8mL/min
1.
2.
3.
4.
1.5
2 Min.
Agilent 1200
(45:55) 20mM Potassium P
hosphate,
pH 2.5:Acetonitrile
UV at 254nm
Ambient
0
0
10
14 Min.
12
Efficiency and Retention Times Remain Constant after Exposure to 12,000psig and 2000 Injections
1.
2.
3.
4.
5.
6.
Column Stability Comparison, 50 x 2mm

1
150
100
8 Min.
5
2+3
1
17140
16283
15426
14569
12855
13712
11141
9427
10284
7713
8570
6856
5142
6000
4285
3428
2571
1714
857
Competitor
Sub 2m Column
50
Initial
200
11998
Plates/Meter (000)
250
2+3
VisionHT Column 5
VisionHT Column 6
VisionHT Column 7
Competitor Sub 2m Column
VisionHT Column 1
VisionHT Column 2
VisionHT Column 3
VisionHT Column 4
300
Uracil
Pyridine
Phenol
Methyl Alanine
4-Butyl Benzoic Acid
Toluene
FINAL
(After 2000 injections)
Column Volumes
8 Min.
Column:
2.0 x 50mm
Mobile Phase: 50mM KH2PO4, pH 7.0:CH3CN (60:40)
Flow Rate:
1.0mL/min
High-pressure competitor sub 2m column lost efficiency at routine
12,000psig pressures. VisionHT columns remain stable.
34
Before and after chromatograms show a constant level of

performance after 2000 injections.
analytical hplc

Comprehensive High Purity 1.5m Selectivity Options
VisionHT phases have exceptionally rigid silica structure that withstands

routine use of 12,000psig pressure often required in this new technology.
Reproducible silica synthesis, bonding and column packing guarantee
low bleed and excellent column-to-column consistency.
VisionHT Phase Specifications
Packing
Base
Material
Particle
Size
C18-HL Spherical 1.5m

Silica
C18-B Spherical 1.5m
Silica
Carbon Pore
Load
Size
Surface
Area
Endcapped
pH
Range* Feature
110
10%
120 220m /g
Yes
5%
120 220m2/g
Proprietary 110
Spherical 1.5m
Silica
Spherical 1.5m
Silica
6%
100 200m2/g
Yes
110
5%
100 200m /g
No
110
HILIC
Spherical 1.5m
Silica
NA
120 220m2/g
No
28
Silica
Spherical 1.5m
Silica
NA
120 220m2/g
No
28
C18
C18-P
7443
Benefits
Fully bonded silica, ultra

high purity silica
High purity silica and
unique endcapping.
High capacity for hydrophobic compounds. Good

peak symmetry.
Improved performance of basic compounds at
neutral pH. Better sensitivity and peak shape by
mass spec for basic compounds, without the need
for acidified mobile phases.
Moderate silica exposure Classic reversed-phase selectivity. Reduced
bonding is optimized for speed and sensitivity.
High silica exposure, low Unique polar selectivity. Low carbon load gives
carbon load
fastest reversed-phase elution times while retaining
polar compounds longer.
Polar phase with shorter Peak reversal compared to reversed-phase. Ideal
equilibration times.
for very polar compounds with high organic mobile
Shipped in ACN/Water.
phases for improved sensitivity by MS.
Traditional normal phase For isomeric separation of non-aqueous compatible
for use in 100% organic compounds by absorption chromatography.
mobile phases
Sub 2m particles deliver efficiency and speed, but critical to success is

having the right stationary phase selectivity. Six VisionHT high purity phases
are available, each with unique separation benefits. C18-HL, with maximum
bonded phase coverage, is ideal for complex hydrophobic samples. Use C18-B
for basic compounds at neutral pH, often a requirement for mass spec work in
the pharmaceutical industry. Reserve the C18 and C18-P for fastest analysis
times. Both offer increased polar interactions to make neutral, non-polar
compounds elute faster and retain polar compounds longer. The HILIC and
Silica packings are normal phases that typically use near exclusive organic
mobile phases; an advantage when seeking highest mass spec sensitivity.
*Choice of buffer is critical at pH >8.
VisionHT Selectivity
VisionHT Reversed-Phase
Selectivity
10
9
8
k' Phenol
k' Methyl Alanine
k' Butyl Benzoic Acid
k' Toluene
Separate Highly Basic Components at

Neutral pH with Vision HT C18-B
3
1. Phenylephrine
2. Diphenhydramine
3. Amitriptyline
k' 5
4
3
2
1
0
C18-P
C18
C18-B
C18-HL
VisionHT Phase
0
1
2
Column:
Mobile Phase:
Column Temp:
Flow Rate:
Detector:
3
4
5 Min.
VisionHT C18-B, 1.5m, 2.0 x 50mm
50mM Ammonium Formate pH 7:Methanol (20:80)
40C
0.2mL/min
UV at 210nm
technical assistance
Contact Tech Support: Phone: 1.800.255.8324 (North America)

Email: [email protected]

Online: www.discoverysciences.com
35
analytical hplc
Unique Column Hardware Advantages
VisionHT Hardware Specifications

Dead volume:
<15nL
Wetted materials:
316 Stainless Steel, PTFE
Port Geometry:
1032, for Industry Standard &
Waters connection
Pressure rating, hardware alone:
18,000psig (1250 bar)
Pressure rating, packed column:
12,000psig (830 bar)
i.d.:
1.0, 2.0mm
Length:
20, 30, 50, 100mm
You can erode the benefits of sub 2m media if packed in

an inefficient column design. VisionHT hardware uses
thin screens, instead of traditional frits, to retain media and
minimize dead volume. A unique insert combination seals
endfittings leak-free to 18,000psig. Various dimensions offer
even more flexibility over analysis speed and resolution.
7136
The Best Value for Ultra High-Pressure Method DevelopmentUltraMD Kits!

Whether developing a new method, or improving an existing analysis, the VisionHT UltraMD kits can help optimize your
separation. Get four reversed-phase columns with varying selectivity for the price of three, and develop your fast LC method fast!
VisionHT UltraMD Kits
UltraMD Kit 1
UltraMD Kit 2
UltraMD Kit 3
Phases
Dimensions
Part No.
C18, C18-P, C18-HL, C18-B

C18, C18-P, C18-HL, C18-B
C18, C18-P, C18-HL, C18-B
2 x 100mm
2 x 50mm
1 x 50mm
5142692
5142693
5142691
7455
VisionHT Reversed-Phase Comparison

5
5
2+3
5
1.
2.
3.
4.
5.
6.
5+6 3 1
C18-HL
31
31
4
C18
2 4
C18-P
2
4
0
36
Uracil
Pyridine
Phenol
Methyl Alanine
Toluene
C18-B
2
6 Min.
Column:
2.0 x 50mm
Mobile Phase: 50mM KH2PO4, pH 7.0:CH3CN (60:40)
Flow Rate:
1.0mL/min
analytical hplc

VisionHT Ultra High-Pressure Columns
The smaller diameters and finer porosity frits required in

UHPLC creates a higher likelihood of column and system
blockage. Trace contaminates not normally problematic
for traditional LC, may now pose backpressure issues.
VisionHT guards protect your column to minimize down
time and reduce cost.
C18-HL, 1.5m Ultra High-Pressure

Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
C18-B, 1.5m Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
C18, 1.5m
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
C18-P, 1.5m Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
HILIC, 1.5m
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
SI, 1.5m
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Ultra High-Pressure
Packing
Two guard formats available: Integral and stand-alone.

Couple the zero-dead volume integral guard directly to
any VisionHT column, and realize absolutely no loss in
efficiency. Use the stand-alone version to protect other
manufacturers UHPLC columns, or as an on-line enrichment
device.
Prevent Column Blockage with Integral Guards
N=174K/m
with guard
without guard
0
0.5
1.0
1.5
2.0 Min.
No loss in efficiency.
VisionHT Integral Guard System
7456
Replace existing endfitting with the integral guard endfitting. Change

guard cartridges as necessary.
Stand-Alone Guard System
Format
i.d. x Length
Part No.
1 x 20mm
1 x 30mm
1 x 50mm
1 x 100mm
2 x 20mm
2 x 30mm
2 x 50mm
2 x 100mm
1 x 30mm
1 x 50mm
1 x 100mm
1 x 20mm
2 x 20mm
2 x 30mm
2 x 50mm
2 x 100mm
1 x 20mm
1 x 30mm
1 x 50mm
1 x 100mm
2 x 20mm
2 x 30mm
2 x 50mm
2 x 100mm
1 x 20mm
1 x 30mm
1 x 50mm
1 x 100mm
2 x 20mm
2 x 30mm
2 x 50mm
2 x 100mm
1 x 20mm
1 x 30mm
1 x 50mm
1 x 100mm
2 x 20mm
2 x 30mm
2 x 50mm
2 x 100mm
1 x 50mm
1 x 100mm
2 x 50mm
2 x 100mm
5142540
5142541
5142542
5142543
5142544
5142545
5142546
5142547
5141903
5141904
5141905
5141902
5141906
5141907
5141908
5141909
5139555
5139559
5139603
5139607
5139557
5139600
5139605
5139609
5139556
5139601
5139604
5139608
5139558
5139602
5139606
5139610
5141910
5141912
5141913
5141914
5141916
5141917
5141919
5141920
5141921
5141923
5141922
5141924
Protect Your UHPLC Column Investment
VisionHT Guard Cartridges

Packing
7457
In-line guard system expands applications beyond column protection.

Use as a sample enrichment device or to protect any uhplc
commercially available column.
Format
i.d. x Length
Ultra High-Pressure
1 x 5mm
Ultra High-Pressure
2 x 5mm
C18 B, 1.5m
Ultra High-Pressure
1 x 5mm
Ultra High-Pressure
2 x 5mm
C18, 1.5m
Ultra High-Pressure
1 x 5mm
Ultra High-Pressure
2 x 5mm
C18-P, 1.5m Ultra High-Pressure
1 x 5mm
Ultra High-Pressure
2 x 5mm
HILIC, 1.5m
Ultra High-Pressure
1 x 5mm
Ultra High-Pressure
2 x 5mm
SI, 1.5m
Ultra High-Pressure
1 x 5mm
Ultra High-Pressure
2 x 5mm
Integral Guard Column Holder For VisionHT
Stand-Alone Guard Holder
C18HL, 1.5m
Qty.
Part No.
3/pk
3/pk
3/pk
3/pk
3/pk
3/pk
3/pk
3/pk
3/pk
3/pk
3/pk
3/pk
5142549
5142548
5141953
5141952
5141950
5141594
5141951
5141595
5141955
5141954
5141957
5141956
3118351
3118350
37
analytical hplc
Alltech Alltima HP Introduction
High-Stability, High-Purity, High-Performance, Low-Bleed Columns for

Demanding Applications
Better Peak Symmetryhigh-purity silica eliminates peak tailing problems
Long Column Lifeexceptional column stability minimizes downtime and reduces cost
Ideal for Microbore or Critical Analysislow to no detectable column bleed
Variety of Phases and Formatsoptimizes retention, resolution, and analysis time
Alltima HP combines our best phase chemistries with high-purity silica. The result is
one product family with selectivity and performance needed to overcome your most challenging
separation needs.
Alltech Alltima HP Phase Specifications
Phase
Description
Base
Particle
Particle Size
Pore
Size
Surface
Area
Carbon
Load
Phase
Endcapped? USP
C18
C18 EPS
C18 HiLoad
C18 AQ
C18 Amide
For classic reversed-phase applications

Reversed-phase C18 with extended polar selectivity
Higher carbon load for stronger retention
100% water wettable
Low bleed polar-embedded phase compatible
with microbore
For reversed-phase applications where C18 is
too retentive
Stable, long-life cyano phase
For general purpose normal-phase applications
Hydrophillic Interaction Chromatography for highly
polar analytes
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
190
190
100
100
190
200 m /g
200 m2/g
450 m2/g
450 m2/g
200 m2/g
12%
4%
24%
20%
12%
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Yes
Yes
Yes
Yes
Yes
L1
L1
L1
L1
L1
Silica Spherical 3, 5m
190
200 m2/g
8%
Monomeric Yes
L7
Silica Spherical 1.5, 3, 5m
190
100
120
200 m2/g
450 m2/g
230 m2/g
4%
Monomeric Yes
No
L10
L3
L3
C8
Cyano
Silica
HILIC
Basic Compounds at Neutral pH

2
3
Peak
USP Tailing*
1. Diphenhydramine
2. Nortriptyline
3. Amitriptyline
1.29
1.41
1.27
*Tailing Factor was determined in accordance with USP method.
Column:
Mobile Phase:
Flow Rate:
Detector:
1
0
Alltima HP C18, 5m, 4.6 x 150mm

25mM Potassium Phosphate, pH 7.0:Methanol (15:85)
1.0mL/min
UV at 254nm
7 Min.
The Alltima HP phases demonstrate excellent peak symmetry of highly basic analytes at pH 7.0.
Basic Compound Stability at pH1 and pH10

2 3
2
2
31
Retention Times
1. Uracil
2. Phenol
3. N,N-Diethyl-m-Toluamide
4. Toluene
3
4
Column:
Mobile Phase:
Flow Rate:
Detector:
1
1
Final Acid
Final Base
10252
0.55
0.81
1.08
1.88
0.55
0.81
1.08
1.89
Alltima HP C18, 5m, 4.6 x 50mm

Acetonitrile:Water (58:42)
1.0mL/min
UV at 254nm
Final Base: washed with 24,000 column volumes of Acetonitrile:Ammonium

Hydroxide, pH 10 (50:50) at 20C.
3 Min.
The Alltima HP phases demonstrate exceptional stability from pH 1.0 to 10.
38
10250
0.56
0.82
1.08
1.89
Final Acid: washed with 24,000 column volumes of Acetonitrile:Dilute Sulfuric Acid,
pH 1.0 (50:50) at 60C.
Initial
0
10249
analytical hplc
Alltech Alltima HP Introduction

The Alltima HP Family Demonstrates Low Bleed
Alltima HP columns are among the lowest-bleed columns available, making them ideal for microbore applications. Our C18
Amide polar-embedded phase has significantly lower column bleed than competitive amide columns.
Column Bleed by ESI-MS Alltima HP vs. Competitive Columns

14
13.2
11.8
Relative Abundance Level (X105)
12
Alltech Alltima HP
10
Competitors
8
6
4
2
2.91
0.82
0.82
0.82
2.52
1.2
0.82
Alltima HP Method Development Kits
1
8A
mi
de
de
mi
8A
C1
C1
All
t
C1 ima
8A H
mi P
de
S2
C1
8M
S1
8M
C1
All
C1 tima
8H
iLo HP
ad
a
H
C1 P
8
All
tim
a
Sil HP
ica
tim
All
Co
lum No
n
best
value
Three Different Kits with Different Selectivities

Whether you are developing a new method, or just not
satisfied with an existing separation, the Alltima HP family
can help optimize your separation. Your best value for method
developmentpurchase a kit and receive three columns for
the price of two.
6113
6904
7345
Alltima HP Method Development Kits

Description
Kit 1 C18 Mix, 3m

Expedite MS Kit 1
Analytical Kit 1
Rocket Kit 1
Kit 2 Alternate Mix, 3m
Expedite MS Kit 2
Analytical Kit 2
Rocket Kit 2
Kit 3 Polar Mix, 3m
Expedite MS Kit 3
Analytical Kit 3
Rocket Kit 3
Includes:
i.d. x Length
Part No.
C18, C18 HiLoad, C18 Amide (87674, 87692, 87728)

C18, C18 HiLoad, C18 Amide (87668, 87686, 87722)
C18, C18 HiLoad, C18 Amide (87672, 87690, 87726)
2.1 x 20mm
4.6 x 150mm
7 x 53mm
87854
87850
87852
C18 HiLoad, C8, CN (87692, 87746, 87764)

C18 HiLoad, C8, CN (87686, 87740, 87760)
C18 HiLoad, C8, CN (87690, 87744, 87762)
2.1 x 20mm
4.6 x 150mm
7 x 53mm
87864
87860
87862
C18 EPS, C18 Amide, CN (87710, 87728, 87764)

C18 EPS, C18 Amide, CN (87704, 87722, 87758)
C18 EPS, C18 Amide, CN (87708, 87726, 87762)
2.1 x 20mm
4.6 x 150mm
7 x 53mm
87874
87870
87872
tech tip
Want high throughput on a conventional (non-ultra

high-pressure) system?
Choose the Rocket column formatno modifications
necessary to your existing HPLC system. Choose Expedite
columns for low volume and microbore systems. See page 31
for more explanation of the Rocket and Expedite formats.
39
analytical hplc
Alltech Alltima HP
Reversed-Phase Columns
Selectivity Options in One Product Family

C18:
Classic reversed-phase retention and selectivity
C18 AQ:
100% water wettable and high carbon loading for greater
mobile phase range
EPS C18:
Unique selectivity that succeeds when traditional
reversed-phase columns fail
Greater retention and enhanced peak symmetry for polar
compounds
C18 HiLoad:
Superior retention and loadability for resolution of
complex samples
C18 Amide:
The first polar-embedded packing ideal for microbore
Excellent peak shape without phase bleed
Ideal for basic compounds in neutral to alkaline pH
C8:
Less hydrophobic retention than C18
Alltima HP HPLC Columns

Packing
Format
i.d. x Length
Part No.
C18, 3m*
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent-Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
Microbore
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Expedite MS
Expedite MS
Microbore
Microbore
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
Microbore
Microbore
Analytical
Analytical
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent-Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
Microbore
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
0.150 X 50mm
0.150 X100mm
0.150 X150mm
0.300 X 50mm
0.300 X100mm
0.300 X150mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
0.150 x 50mm
0.150 x 100mm
0.150 x 150mm
0.300 x 50mm
0.300 x 100mm
0.300 x 150mm
2.1 x 10mm
2.1 x 20mm
2.1 x 100mm
2.1 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
2.1 x 150mm
2.1 x 250mm
4.6 x 150mm
4.6 x 250mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
22153
22154
22155
22156
22157
22158
87673
87674
87504
87669
87670
87601
87675
87676
87826
87667
87668
87671
87672
87681
87682
87602
87603
87679
87680
22560
22561
22562
22563
22564
22565
87813
87814
87809
87810
87815
87816
87832
87807
87808
87811
87812
87821
87822
87819
87820
87709
87710
87508
87705
87706
87604
87711
87712
87833
87703
87704
87707
87708
87717
87718
87605
87606
87715
87716
C18, 5m
Alltima HP Selectivity
14
13
12
11
10
9
8
k' 7
6
5
4
3
2
1
0
k' Toluene
C18 AQ, 3m*
k' Butyl Benzoic Acid

k' Amitriptyline
CN
C18 EPS
C8
C18
C18
Amide
Alltima HP Packing
Alltima HP Packing
C18 AQ
C18
HiLoad
C18 AQ, 5m
Acid, Base, and Neutral Test Mix

5
1.
2.
3.
4.
5.
6.
5
5
12
12
3
2
1
4
3 4
6
40
C18 HiLoad
C18
4
6
8 Min.
4.6 x 150mm
50mM KH2PO4, pH 3.0:CH3CN (40:60)
Ambient (22C)
1.0mL/min
5L
UV at 254nm
C18 EPS, 3m
EPS C18
6
0
2
Column:
Mobile Phase:
Column Temp:
Flow Rate:
Injection Vol.:
Detector:
Uracil
Pyridine
Phenol
N,N-diethyl-m-toluamide
Toluene
C18 EPS, 5m
*Other particle sizes and dimensions are available.
analytical hplc
Alltech Alltima HP Reversed-Phase Columns

Alltima HP HPLC Columns (continued)
Alltima HP-Guard Cartridges
Format
i.d. x Length
Part No.
Packing
C18 Hi-Load,
3m*
Capillary
Capillary
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent-Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
Microbore
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
Capillary
Capillary
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent-Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
Microbore
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent-Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
Microbore
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
0.150 x 50mm
0.300 x 50mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
0.150 x 50mm
0.300 x 50mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
0.150 x 50mm
0.150 x 100mm
0.150 x 150mm
0.300 x 50mm
0.300 x 100mm
0.300 x 150mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
22190
22193
87691
87692
87506
87687
87688
87610
87693
87694
87827
87685
87686
87689
87690
87699
87700
87611
87612
87697
87698
22255
22258
87727
87728
87510
87723
87724
87607
87729
87730
87829
87721
87722
87725
87726
87735
87736
87608
87609
87733
87734
22479
22480
22481
22482
22483
22484
87745
87746
87512
87741
87742
87613
87747
87748
87830
87739
87740
87743
87744
87753
87754
87614
87615
87751
87752
C18 Capillary Guard, 3m**
C18 Hi-Load,
5m
C18 Amide,
3m*
C18 Amide,
5m
C8, 3m*
C8, 5m
i.d. x Length
0.150 x 10mm
0.300 x 10mm
C18 All-Guard, 5m*
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
C18 AQ Capillary Guard, 3m** 0.150 x 10mm
0.300 x 10mm
C18 AQ All-Guard, 5m*
2.1 x 7.5mm
4.6 x 7.5mm
C18 EPS All-Guard, 5m*
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
C18 Hi-Load Capillary
0.150 x 10mm
Guard, 3m**
0.300 x 10mm
C18 Hi-Load All-Guard, 5m* 2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
C18 Amide Capillary
0.150 x 10mm
Guard, 3m**
0.300 x 10mm
C18 Amide All-Guard, 5m*
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
C8 Capillary Guard, 3m**
0.150 x 10mm
0.300 x 10mm
C8 All-Guard, 5m*
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
All-Guard Cartridge Holder
(Includes Direct-Connect Column Coupler)
Capillary Guard Cartridge Holder
Guard Holder for 0.100mm and 0.150mm Guards
Qty.
Part No.
ea
ea
3
3
3
ea
ea
3
3
3
3
3
ea
ea
3
3
3
ea
ea
3
3
3
ea
ea
3
3
3
ea
22578
22579
87683
87622
87684
22638
22639
87823
87824
87719
87623
87720
22588
22589
87701
87624
87702
22598
22599
87737
87625
87738
22648
22648
87755
87627
87756
80101
ea
ea
GR-3710E
GR-3710A
Packing
*All-Guard holder required.

**Other particle sizes and dimensions are available.
tech tip
Which guard format should I choose?

Ideally, the guard should have the same or slightly smaller i.d. as
the HPLC column. Efficiency will suffer, if the guard i.d. is larger
than that of the column.


*Other particle sizes and dimensions are also available.
41
analytical hplc
Alltech Alltima HP HILIC Columns
Hydrophilic Interaction (HILIC) Columns for

Highly Polar Basic Analytes
Mixed Analyte HILIC
Superior retention of highly polar compounds

Increased sensitivity and lower detection limits
with microbore
Available in 1.5m particle size
Hydrophilic Interaction Chromatography is ideal for
separating and retaining very polar compounds that may
not retain on traditional reversed-phase packings. Unlike
reversed-phase columns, HILIC columns retain highly polar
compounds with only small amounts of water in the mobile
phase. These more volatile mobile phases increase sensitivity
with microbore applications.
Basic Drugs
10949
1
2
10461
Reverse-Phase
C18 Columns
1. Toluene
2. Hydantoin
3. Uracil
4. Caffeine
5. Allantoin
5
3
1
5 Min.
Column:
lltima HP HILIC, 5m, 4.6 x 150mm
A

(Part No. 86464)
Mobile Phase: Acetonitrile:0.10M Ammonium Formate,
pH 3.0 (95:5)
Flow Rate:
1.0mL/min
Detector:
UV at 205nm
Alternate selectivity compared to reversed-phase columns allows
more separation possibilities.
1. Albuterol
2. Bamethan
Alltima HP Hilic HPLC Columns

Packing
Format
i.d. x Length
Part No.
HILIC, 1.5m
Expedite MS
Expedite MS
Rocket
Rocket
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Expedite MS
Expedite MS
Microbore
Microbore
Expedite MS
Expedite MS
Analytical
Analytical
Rocket
Rocket
Microbore
Analytical
Analytical
2.1 x 20mm
4.6 x 20mm
7 x 33mm
7 x 53mm
0.150 x 50mm
0.150 x 100mm
0.150 x 150mm
0.300 x 50mm
0.300 x 100mm
0.300 x 150mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 150mm
7 x 33mm
7 x 53mm
2.1 x 150mm
4.6 x 150mm
4.6 x 250mm
86471
86472
86467
86468
22515
22516
22517
22518
22519
22520
86473
86475
86461
86463
86474
86476
86460
86462
86469
86470
86465
86464
86466
2
1
HILIC, 3m*
Alltima HP
HILIC Columns
0 1 2 3 4 5 6 7 8 9 10 Min.
Column:
revail C18 Column, 5m, 4.6 x 150mm

P
(Part No. 99208)
Alltima HP HILIC Column, 5m, 4.6 x 150mm,
(Part No. 86464)
Mobile Phase: 0.5M Ammonium Formate, pH 3.0:CH3CN (88:12)

Acetonitrile:0.5M Ammonium Formate, pH 3.0 (85:15)
Flow Rate:
1.0mL/min
Detector:
UV at 220nm
Use Alltima HP HILIC's alternate selectivity for greater separation
possibilities.
HILIC, 5m
*Other particle sizes and dimensions are available.
tech tip

columns for low volume and microbore systems. See page 31
for more explanation of the Rocket and Expedite formats.
Expedite
Columns
Rocket
Columns
6904
42
6113
Alltima HP HILIC Guard Cartridges

Packing
i.d. x Length
Qty.
Part No.
HILIC Capillary Guard,

3m**
HILIC All-Guard, 5m*
0.150 x 10mm
0.300 x 10mm
2.1 x 7.5mm
4.6 x 7.5mm
ea
ea
3
3
ea
22658
22659
86479
86480
80101
ea
ea
GR-3710E
GR-3710A

**Other particle sizes and dimensions are available.
analytical hplc
Alltech Alltima HP Normal-Phase Columns

Cyano: Superior stability

More reproducible separations than silica for
normal-phase applications

Ideal for basic drug analysis
Silica: Normal phase
Corticosteroids
Alltima HP Cyano
Much More Stable than Other Cyanos
-35
10391
pH 1.0
-30
% Changes in Retention
5. Prednisone
6. Hydrocortisone
7. Prednisolone
1. Deoxycorticosterone Acetate
2. Hydrocortisone Acetate
3. Cortisone Acetate
4. Cortisone
3 4
pH 7.5
-25
-20
-15
0
-10
9 10 11 12 13 Min.
-5
-0
a
ltim
Al
N
PC
an
Br
N
AC
an
Br
N
BC
an
Br
N
CC
dD
an
Br
CN
an
Br
N
EC
an
Br
N
FC
QC D4 Test:
7 0:30 H2O/CH3CN,1.0mL/min, UV at 254nm, 5L

D4Mix, Column 4.6 x 150mm, 22C
pH 1.0 Stability: Flushed with H2SO4 pH 1.0:Acetonitrile 50:50 at
1.0mL/min for 3 days
pH 7.5 Stability: Flushed with 20mM K2HPO4 pH 7.5:Acetonitrile
50:50 at 1.0mL/min for 3 days
Column:

Mobile Phase:
Flow Rate:
Column Temp:
Detector:
Alltima HP HPLC Columns (continued)

Packing
Format
i.d. x Length
Part No.
Silica, 5m
Microbore
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
87799
87802
87620
87621
87797
87798
Alltima HP HPLC Columns

Packing
Format
i.d. x Length
Part No.
Cyano, 3m
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent-Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
Microbore
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent-Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
87763
87764
87514
87759
87760
87616
87765
87766
87825
87757
87758
87761
87762
87781
87782
87617
87618
87769
87780
87791
87792
87516
87787
87788
87619
87793
87794
87831
87785
87786
87789
87790
Cyano, 5m
Silica, 3m
Alltima HP CN, 5m, 4.6 x 150mm

(Part No. 87769)
Hexane:Isopropanol (93.7:6.3)
2.0mL/min
35C
UV at 254nm
Alltima HP All-Guard Cartridges*

Packing
i.d. x Length
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
Silica, 5m
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
Cyano, 5m
Qty.
Part No.
3
3
3
3
3
3
ea
87783
87626
87784
87803
87628
87804
80101
*All-Guard holder required. Other particle sizes available.
more applications
To view our complete searchable

chromatogram database visit
www.discoverysciences.com/chromdb/
43
analytical hplc
Alltech Prevail Introduction
Stable from 100% Organic to 100% Aqueous

Long column life in both highly aqueous and highly organic mobile phases
Excellent retention and reproducibility of highly polar analytes in 100% aqueous
mobile phases
Strong retention of hydrophobic analytes in 100% organic mobile phases, no concerns
of solubility, better sensitivity in microbore and ELSD applications
No phase collapse
Specialty phases for specific applications
With one Prevail C18 column, retain polar compounds in aqueous mobile phases and hydrophobic compounds in organic
mobile phases.
Alltech Prevail Phase Specifications
Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
C18 Select
C18
C8
Phenyl
Cyano
Amino (NH2)
Silica
Organic Acid
Carbohydrate ES
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Polymer
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
5m
110
110
110
110
110
110
110
110
350m2/g
350m2/g
350m2/g
350m2/g
350m2/g
350m2/g
350m2/g
350m2/g
17%
15%
8%
7%
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Yes
Yes
Yes
Yes
Yes
No
Yes
L1
L1
L7
L11
L10
L8
L3
Switch Between 100% Aqueous and 100% Organic Mobile Phases on the Same Column
Water-Soluble Vitamins
(100% Aqueous)
Fat-Soluble Vitamins and Tocopherols

(100% Organic)
9465
1. Vitamin A
2. -Tocopherol
3. -Tocopherol
4. -Tocopherol
(Vitamin E)
10 12 14 Min.
Column:
revail C18, 5m,

P
4.6 x 150mm
Mobile Phase: 25mM KH2PO4
pH 3.0:CH3CN (97:3)
Flow Rate:
1.0mL/min
Detector:
UV at 212nm
Figure 1
wash with
intermediate
Column:
10
12
revail C18, 5m,

P
4.6 x 150mm
Mobile Phase: CH3CN:CH3OH (75:25)
Flow Rate:
1.5mL/min
Detector:
UV at 220nm
Figure 2
wash with
intermediate
2 3
1. Thiamine
2. Ascorbic Acid
3. Nicotinic Acid
4. Pyridoxine
5. Niacinamide
6. Pantothenic Acid
5. Vitamin D2
6. Vitamin D3
7. Vitamin K
4
2
9465
9405
1. Thiamine
2. Ascorbic Acid
3. Nicotinic Acid
4. Pyridoxine
5. Niacinamide
6. Pantothenic Acid
Water-Soluble Vitamins
(100% Aqueous)
14 Min.
5
6
10 12 14 Min.
Column:
revail C18, 5m,

P
4.6 x 150mm
Mobile Phase: 25mM KH2PO4
pH 3.0: CH3CN (97:3)
Flow Rate:
1.0mL/min
Detector:
UV at 212nm
Figure 3
Prevail Columns have highly stable bonded phases that let you use one column for multiple mobile phase conditions. Even switching between
extremes such as near 100% aqueous (Figure 1) to 100% organic conditions (Figure 2), and back to near 100% aqueous (Figure 3) on a routine
basis is possible, provided that mobile phases are miscible.
44
analytical hplc
Alltech Prevail Reversed-Phase Columns

Prevail HPLC Columns
Prevail HPLC Columns (continued)
Format
i.d. x Length
Part No.
Packing
Format
i.d. x Length
Part No.
Select C18,
3m
Microbore
Microbore
Microbore
Solvent-Reducer
Analytical
Analytical
Rocket
Rocket
Microbore
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
Microbore
Microbore
Microbore
Microbore
Microbore
Microbore
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent-Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
150m x 50mm
150m x 150mm
300m x 50mm
300m x 150mm
1.0 x 100mm
1.0 x 150mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
99309
99312
99313
99315
99302
99303
99304
99305
99310
99311
99316
99317
99300
99301
35762
35764
32831
32832
43831
43843
43861
43827
43818
43871
99200
99322
43878
43804
43829
99202
99204
99280
99279
C18, 5m
Microbore
Microbore
Microbore
Microbore
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
Analytical
Microbore
Solvent-Reducer
Analytical
Analytical
Analytical
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
Microbore
Microbore
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent-Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
150m x 50mm
150m x 150mm
300m x 50mm
300m x 150mm
2.1 x 150mm
3.0 x 150mm
3.0 x 250mm
4.6 x 50mm
4.6 x 150mm
4.6 x 250mm
2.1 x 150mm
3.0 x 150mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
2.1 x 150mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
1.0 x 100mm
1.0 x 150mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
2.1 x 150mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
35792
35794
32815
32816
99206
99320
99321
43903
99208
99210
99212
99325
43922
99214
99216
99218
99323
99324
99224
99229
43893
43859
43873
43885
43819
43872
99231
99328
43887
43815
43869
99233
99235
99282
99281
99237
99326
99327
99239
99241
Select C18,
5m
C18, 3m
tech tip
C8, 3m
C8, 5m
Phenyl, 3m

columns for low volume and microbore systems. See page 31 for
more explanation of the Rocket and Expedite formats.
9450
9451
Phenyl, 5m
Prevail Bonded Phases are Stable in 100% Aqueous Conditions

Prevail C18, 5m, 4.6 x 150mm
1
Catecholamines
1. Norepinephrine
2. Epinephrine
3. 5-Hydroxydopamine
9452
9454
9455
2
2
Mobile Phase: 25mM KH2PO4, pH 2.7

Flow Rate:
1.0mL/min
Detector:
UV at 270nm
9453
Competitor A C18, 5m, 4.6 x 150mm
1
1
Packing
3
3
After 24 hrs.
After 8 hrs.
After 20 min.
6 Mi
6 Mi
45
analytical hplc
Alltech Prevail Normal-Phase Columns
Prevail HPLC Columns (continued)
Packing
Format
i.d. x Length
Part No.
Packing
Format
i.d. x Length
Part No.
Cyano, 3m
Microbore
Solvent-Reducer
Analytical
Analytical
Analytical
Microbore
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Microbore
Microbore
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent-Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
2.1 x 150mm
3.0 x 150mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
2.1 x 150mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
1.0 x 100mm
1.0 x 150mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
99243
99338
43924
99245
99247
99249
99329
99330
99251
99253
43926
99257
99259
99263
99265
43832
43806
43841
43826
43868
43805
99267
99341
43858
43816
43842
99269
99271
99284
99283
Silica, 5m
Analytical
Solvent-Reducer
Solvent-Reducer
Analytical
Analytical
2.1 x 150mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
99273
99339
99340
99275
99277
Cyano, 5m
Amino, 3m
Amino, 5m
Silica, 3m
Prevail All-Guard Cartridges*

Packing
i.d. x Length
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
C18, 5m
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
C8, 5m
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
Phenyl, 5m
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
Cyano, 5m
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
Amino, 5m
4.6 x 7.5mm
Silica, 5m
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
Select C18, 5m
Qty.
Part No.
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
ea
96690
99119
96455
96682
99350
99286
99128
99351
99287
99130
99352
99288
99131
99353
99289
99290
99133
99354
99291
80101
Alltech Prevail Organic Acid Columns

Get Unsurpassed Resolution for Common
Organic Acids
Figure 1 - Organic Acids

1
Acid stable for long column lifetimes

Silica-based for maximum efficiency and high-resolution
Short run times and high sample throughput at ambient
temperature with Rocket format
Lower cost than polymeric organic acid columns
Prevail Organic Acid (OA) columns separate common
organic acids with an unsurpassed combination of resolution,
speed, sensitivity, and simplicity. A simple acidic phosphate
buffer and a Prevail OA column at ambient temperature will
separate 11 short-chain organic acids in less than 6 minutes.
Use pH to adjust column selectivity. Lowering the mobile
phase pH progressively suppresses the ionization of the
carboxylic acids, making them more hydrophobic. This gives
you the ability to move these peaks relative to other peaks in
the chromatogram, and it simplifies method development.
46
9384
11
2
3
56
Column:

Mobile Phase:
Flow Rate:
Detector:
10
8 Min.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
Oxalic Acid
Tartaric Acid
Formic Acid
Malic Acid
Lactic Acid
Acetic Acid
Diglycollic Acid
Maleic Acid
Citric Acid
Succinic Acid
Fumaric Acid
Prevail Organic Acid, 5m, 4.6 x 150mm

(Part No. 88640)
25mM KH2PO4, pH 2.5
1.5mL/min
UV at 210nm
Separate 11 short-chain organic acids in less than six minutes.

Packing
Format
i.d. x Length
OA, 3m
Microbore
Analytical
Analytical
Rocket
Rocket
Analytical
Analytical
2.1 x 100mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
4.6 x 150mm
4.6 x 250mm
OA, 5m
Standard
Part No.
Waters
Fittings
Part No.
88648
88650
88655
99292
50755
88640
88645
88750
88755
88740
88745
Packing
i.d. x Length
Organic Acid, 5m
4.6 x 7.5mm
Qty.
Part No.
3
ea
96429
80101
analytical hplc
Alltech Prevail Carbohydrate ES Columns

Get Better Retention Times, Longer Column Life, and Improved Baselines Compared to
Traditional Amino Carbohydrate Columns
Quiet Gradient Baselines
Better Resolution and Peak Shape
Prevail Carbohydrate ES Columns are excellent for

isocratic separations with common detectors, but the
columns real power shows with the ELSD and a solvent
gradient. Enjoy the powerful selectivity, reduced run times,
and efficient peaks from gradients while seeing the quiet,
stable baselines that maximize sensitivity.
Prevail Carbohydrate ES Columns are versatile

enough for mono- and oligosaccharides and sugar alcohols.
They produce single peaks for reducing sugars at ambient
temperatures, eliminating the need for column heating.
Enhanced Detector Sensitivity Through Flat Gradient Baselines

9354
12
9379
13
1
2
3
2 3
6
78
5 6
14
15
18 19 20 21 22 23 24 Min.
4 5
1. Dextrose
2. Maltose
3. Maltotriose
4. Maltotetraose
5. Maltopentaose
6. Maltohexaose
7. Maltoheptaose
8. Maltooctaose
9. Maltononaose
9486
11 13
10
9 12
Prevail
Carbohydrate ES Column
(Part No. 35101)
Column:
Mobile Phase:
Gradient:

Flow Rate:
Detector:
Silica Based
Amino Column
0
10
15
20
25 Min.
10. Maltodecaose
11. Maltoundecaose
12. Maltododecaose
13. Maltotridecaose
14. Maltotetradecaose
15. Maltopentadecaose
Longer column life: Hybrid phase has the ruggedness of silica with the stability of a polymer
Quieter baselines compared to traditional amino carbohydrate columns, even with gradients
Reduce run times with more mobile phase choices
Compatible with gradient ELSD applications
4.6 x 250mm
A: Acetonitrile B: Water
Time: 0 15
%B: 35 50
1.0mL/min
ELSD
Use gradients with a Prevail Carbohydate ES column and the ELSD for shorter run times, better resolution, and greater sensitivity.
By comparison, silica-based amino columns give noisy, shifting baselines.
Robitussin Flu Syrup
Pepsi One Cola

10530
1.
2.
3.
4.
5.
6.
7.
8.
4
2
10
Column:

Mobile Phase:
Gradient:

Flow Rate:
Detector:
Column Temp:
6
8
15
20
25
30
35
10557
2 4
Sucralose
Unknown 1
Fructose
Glucose
Unknown 2
Sucrose
Maltose
Maltodextrins
1.
2.
3.
4.
5.
6.
3
1
Sucralose
Fructose
Sorbitol
Glucose
Sucrose
Maltose
5 6
40 Min.
Prevail Carbohydrate ES, 5m, 4.6 x 250mm

(Part No. 35101)
Time: 0 5 10 15 25
%B: 20 20 30 30 50
1.0mL/min
ELSD
Ambient

Packing
Format
i.d. x Length
Part No.
Carbohydrate ES,
5m
Analytical
Analytical
Rocket
4.6 x 150mm
4.6 x 250mm
7 x 53mm
35102
35101
35104
10
Column:

Mobile Phase:
Gradient:

Flow Rate:
Detector:
Column Temp:
15
20
25
30
35
40 Min.
Prevail Carbohydrate ES, 5m, 4.6 x 250mm

(Part No. 35101)
Time: 0 5 10 15 25
%B: 15 15 25 25 50
1.0mL/min
ELSD
Ambient

Packing
i.d. x Length
Carbohydrate ES, 5m
4.6 x 7.5mm
Qty.
Part No.
3
ea
96435
80101
47
analytical hplc
Alltech Platinum Columns
For Challenging Separations

Unique selectivity
Better peak shapes with polar analytes
More separation choices with dual-selectivity
Excellent stability and reproducibility
1.5m high throughput media for speed and resolution, especially when combined with
Rocket and Expedite hardware
The Platinum Column Advantage

Controlled silica exposure is the difference that makes Platinum columns unique. Instead of thoroughly
covering the silica with bonded phase to hide the silica, the exposure of the silica in Platinum columns is controlled to provide
a dual mode separation with both polar and non-polar sites exposed to your samples. This extends polar selectivity well beyond
what other reversed-phase columns offer and gives separations other columns cannot.
Standard Platinum Columns vs Platinum EPS Columns

Platinum columns come in two varieties offering different levels of silica exposure. Standard Platinum has a moderate silica
exposure and is best used with neutral and moderately polar compounds. Platinum EPS (Extended Polar Selectivity) has a
high level of silica exposure and is best used with compounds containing more than two polar functional groups.
Alltech Platinum Phase Specifications
Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
C18
EPS C18
C8
EPS C8
Phenyl
Cyano
Amino (NH2)
Silica
SAX
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
1.5, 3, 5m
1.5, 3, 5m
1.5, 3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m*
100
100
100
100
100
100
100
100
100
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
6%
5%
4%
2.50%
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Yes
No
Yes
No
Yes
No
No
No
L1
L1
L7
L7
L11
L10
L8
L3
Trying to solve difficult separation problems using typical reversed-phase columns often leads to the same result. Choose Platinum columns
for completely different selectivity. See chart below.
Hydrophobic/Hydrophillic Balance at pH 3 of Various Reversed-Phase HPLC Packings

1.6
1.4
Platinum
EPS C8
Platinum EPS C18
k Amitripyline / k Toluene
1.2
N O T E
Platinum columns unique selectivity

stands out from the competition.
0.8
Standard
Platinum C8
0.6
Standard
Platinum C18
0.4
Typical RP C8
Typical RP C18
0.2
Typical Polar Embedded

0
k Toluene
Plotting k' values of different compounds (polar vs. nonpolar) demonstrate the unique selectivity of Platinum and Platinum EPS columns,
compared to conventional reversed-phase columns.
48
analytical hplc
Reverse Elution Order with Standard
Platinum and Platinum EPS Columns
Platinum EPS Columns Have

High Polar Compound Capacity
Often, its preferable when minor components elute before,

rather than after, closely retained major components.
This is important for early eluting polar compounds which are

often unresolved on conventional reversed-phase columns.
Vasodilators
1,2
Capacity Factors for Platinum EPS C18 Columns
6.00
1.
2.
3.
4.
5.
Standard Platinum C18

2
4
Column:
Mobile Phase:
Flow Rate:
Detector:
Pindolol
Pentoxifylline
Isoxsuprine
Nifedipine Degradant
Nifedipine
8388
Platinum EPS C18

0
8387
5m, 4.6 x 150mm

0.025M KH2PO4, pH 3.0:Methanol (50:50)
1.0mL/min
UV at 220nm
Format
Rocket
Rocket
C18, 3m
Solvent Reducer
Analytical
Analytical
Rocket
Rocket
C18, 5m
Solvent Reducer
Solvent Reducer
Analytical
Analytical
EPS C18, 1.5m Rocket
Rocket
EPS C18, 3m* Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Solvent Reducer
Analytical
Analytical
Rocket
Rocket
EPS C18, 5m Solvent Reducer
Solvent Reducer
Analytical
Analytical
C8, 1.5m
Rocket
C8, 3m
Rocket
C8, 5m
Analytical
Analytical
EPS C8, 3m
Analytical
Rocket
Rocket
EPS C8, 5m
Analytical
Analytical
C18, 1.5m
5.00
4.00
3.00
2.00
1.00
8 10 12 14 16 Min.
0.00
1
Platinum HPLC Columns

Packing
Capacity Factor
10
15 20
25
30 35
Consecutive Batches
40
Tol
uene
Cinnamic A
Nitrobenzene
Biphenyl
Amitriptyline
45
Platinum HPLC Columns (continued)

i.d. x Length
7.0 x 33mm
7.0 x 53mm
3.0 x 150mm
4.6 x 100mm
4.6 x 150mm
7.0 x 33mm
7.0 x 53mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
7.0 x 33mm
7.0 x 53mm
0.150x 50mm
0.150x100mm
0.150x150mm
0.300x 50mm
0.300x100mm
0.300x150mm
3.0 x 150mm
4.6 x 100mm
4.6 x 150mm
7.0 x 33mm
7.0 x 53mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
7.0 x 53mm
7.0 x 33mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
7.0 x 33mm
7.0 x 53mm
4.6 x 150mm
4.6 x 250mm
Standard Waters Fittings

Part No.
Part No.
50527
50529
32794
32007
32020
50525
50523
32793
32792
32043
32064
50577
50579
22300
22430
22431
22432
22433
22434
32799
32158
32183
50575
50573
32806
32802
32214
32246
50529
50532
32370
32375
32415
50583
50585
32420
32425
*1.5m and 5m particles and other dimensions are available.
32009
32029
32044
32068
32161
32184
32216
32247
32371
32376
32416
32421
32426
Packing
Phenyl, 3m
Phenyl, 5m
Cyano, 3m
Cyano, 5m
Amino, 3m
Amino, 5m
Silica, 3m
Silica, 5m
SAX, 3m
SAX, 5m
Format
i.d. x Length
Standard
Part No.
Waters Fittings
Part No.
Analytical
Analytical
Analytical
Rocket
Rocket
Analytical
Analytical
Analytical
Rocket
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
7.0 x 33mm
7.0 x 53mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
7.0 x 53mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
32631
32636
32641
50593
50595
32672
32681
32706
50545
32713
32722
32535
32542
32549
32952
32944
32943
32632
32637
32642
32675
32682
32707
32714
32723
32536
32543
32550
32953
32946
32945
Platinum Guard Cartridges

Packing
C18 All-Guard, 5m*

EPS C18, 3m
Capillary Guard**
EPS C18 All-Guard, 5m*
i.d. x Length
Qty.
Part No.
3.0 x 7.5mm
4.6 x 7.5mm
0.150 x 10mm
0.300 x 10mm
3.0 x 7.5mm
4.6 x 7.5mm
4.6 x 7.5mm
4.6 x 7.5mm
4.6 x 7.5mm
4.6 x 7.5mm
4.6 x 7.5mm
4.6 x 7.5mm
4.6 x 7.5mm
3
3
3
3
3
3
3
3
3
3
3
ea
99115
32606
22693
22694
99117
32607
32612
32614
32619
32620
32621
32622
32787
80101
ea
ea
GR-3710E
GR-3710A
C8 All-Guard, 5m*
EPS C8 All-Guard, 5m*
Phenyl All-Guard, 5m*
Cyano All-Guard, 5m*
Amino All-Guard, 5m*
Silica All-Guard, 5m*
SAX All-Guard, 5m*
**1.5m and 5m particles and other dimensions are available.
49
analytical hplc
best
value
GraceSmart Columns
new
High purity phases

Efficient and reproducible separations
General use selectivity
Exceptional value
GraceSmart HPLC phases use high-purity silica and are monomerically bonded with uniform
coverage. This translates into symmetrical peaks for acids/bases, and predictable reversed-phase
selectivity. Whether routine analysis or new method development, use GraceSmart columns to
get premium performance at exceptional value.
GraceSmart Phase Specifications
Phase
Encapping
Surface Area
Pore Size
Particle Size
C18
TMS, non-polar
220m2/g
120
3, 5m
Expect Competitive Performance
2,4 Dichlorophenoxy Propionic Acid
2,4 Dichlorophenoxy Butyric Acid

2,4 Dichlorophenoxy Butyric Acid
2.5
1.5
Symmetry
0
1
10
11
Batch
Toluene
N-Butylbenzoic Acid
Anisole
Acetophenone
P-Nitrophenol
Amitriptyline
Consistent batch-to-batch capacity for acidic, basic, and neutral

compounds.
GraceSmart RP18 Columns
Packing
Format
i.d. x Length
C18, 5m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 150mm
4.6 x 250mm
2.1 x 150mm
2.1 x 250mm
4.6 x 150mm
4.6 x 100mm
2.1 x 150mm
2.1 x 100mm
2.1 x 50mm
4.6 x 50mm
Part No.
5138812
5138810
5138811
5138813
5141752
5141753
5141754
5141755
5141756
5141811
12
Symmetry
0.5
C18, 3m
2,4 Dichlorophenoxy Propionic Acid
2,000
2.5
50
4,000
2,4 Dichlorophenoxy Acetic Acid
6,000
1.5
1
0.5
0
Quinine
Capacity Factor
4
3.5
8,000
Nortriptyline
4.5
Efficiency
10,000
Nortriptyline
Diphenhydramine
Plates / Columns
12,000
Diphenhydramine
Highly Reproducible
2,4 Dichlorophenoxy Acetic Acid
Six sigma techniques and lean manufacturing are key to our

cost competitive position, without compromising quality or
performance. GraceSmart columns show similiar or better
efficiency and asymmetries for challenging base and acid
components in comparison to industry leading columns.
Quinine
Our tightly controlled silica synthesis and bonding keep

capacity factor and selectivity variations to a minimum. The
advanced packing methods deliver consistently high columnto-column efficiency. Every column is individually tested and
shipped with a quality assurance chromatogram.
Nicotine
Reproducible Methods Start with

Reproducible Columns
Nicotine
High Quality HPLC Phases at Exceptional Value
Competitor 1 C18 Column


GraceSmart C18 Column
GraceSmart columns compare favorably to leading competitor

columns.
analytical hplc
GraceSmart Columns
Standards Mix
Amoxicillin (U.S.P.)
10696
3500000
3000000
2500000
2000000
1500000
1000000
1.
2.
3.
4.
5.
6.
7.
1
2
Nicotine
Quinine
Diphenhydramine
Nortriptyline
2,4, Dichlorophenoxy Acetic Acid
2,4, Dichlorophenoxy Propionic Acid
2,4, Dichlorophenoxy Butyric Acid
500000
0
-500000
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0 Min.
GraceSmart C18 Column

Luna C18 Column
Inertsil ODS-3 Column
Hypersil BDS C18 Column
Kromasil 100-5 C18 Column
Column:

Mobile Phase:

Flow Rate:
Detector:
Column Temp:
Injection Vol:
Column:
GraceSmart RP18, 5m, 4.6 x 150mm
Mobile Phase: 50% MeCN/50% 50mM, KH2PO4, pH 3 at 35C
Ibuprofen (U.S.P.)
GraceSmart Luna Inertsil

ODS-3
C18
C18
Ret. Time
N/A
(min)
Theoretical 1700
plates
Tailing
2.5
Factor
k'
1.1k'2.8
Hypersil Kromasil
BDS
100-5
C18
C18
3.800
4.050
4.988
3.333
3.768
8543
7216
6230
7049
7680
1.015
1.180
1.041
1.034
0.979
1.2
1.6
2.3
0.99
1.6
2
1. Valerophenone
2. Ibuprofen
2.5
7.5
Column:

Mobile Phase:

Flow Rate:
Detector:
Injection Vol:
10739
Column:

Mobile Phase:
Gradient:

Flow Rate:
Detector:
Injection Vol:
1.
2.
3.
4.
5.
6.
7.
Fenuron
Monuron
Fluometuron
Diuron
Methiocarb
Linuron
Neburon
10733
GraceSmart C18 Column, 5m, 4.6 x 150mm

(Part No. 5138812)
A: 0.025M KH2PO4, pH 3.2 B: Acetonitrile
Time: 0 20
%B: 30 60
1.0mL/min
UV at 240nm
10L

4
3
10 12 14 16 18 20 Min.
more applications
10 12.5 15 17.5 20 Min.

(Part No. 5138812)
H2O (adjusted with Phosphoric Acid to a pH of 2.5):
Acetonitrile (65:35)
2mL/min
UV at 214nm
5L
Water Soluble Vitamins
Carbamates
4
10702

(Part No. 5138810)
Isocratic, 0.05N Monobasic Potassium Phosphate
pH 5:Acetonitrile (96:4)
1.5mL/min
UV at 230nm
30C
10L
USP
Spec
8 Min.
mAu
10697
6000000
5500000
5000000
4500000
4000000
0
2
4
Column:

Mobile Phase:

Gradient:

Flow Rate:
Detector:
Injection Vol:
1.
2.
3.
4.
5.
6.
7.
Ascorbic Acid
Nicotinic Acid
Pyridoxamine
Niacinamide
Folic Acid
Vitamin B12
Riboflavin
6
8
10
12
14 Min.
(Part No. 5138812)
A: 0.1M NH4H2PO4 Buffer, pH 4.5
B: Acetonitrile
Time: 0 4 10 15
%B: 5 5 40 40
1.0mL/min
UV at 254nm
2L
51
analytical hplc
GraceAlpha Columns (Patent Pending)
A New Silica Generation

High-porosity surface increases mass transfer and results in increased column
efficiencies and loading capacity
Dense core and highly spherical shape yields mechanically robust particle
Proprietary bonding technology gives unique selectivity and increased resolution
Ideally suited for scale up to prep applications
new
Extreme Porosity
High-porosity exterior increases mass transfer leading to higher
efficiencies and superior loading capacity.
The high-porosity structure adds an elastic quality to the silica surface
allowing the particles to pack tighter yielding higher efficiencies. It also
stabilizes the packed bed preventing voids.
Dense Inner Core

Dense core provides exceptional strength. Typically the high-porosity
structure would result in a weak particle prone to breaking, but the
GraceAlpha particle combines the high porosity outer region with a dense
inner core that results in a particle that resists breaking and forming fines
that contribute to high backpressures.
GraceAlpha Phase Specifications
Phase
Base Material
Particle Shape
Particle Size
C18
C8
Silica
Spherical
Spherical
Spherical
5, 10, 15, 20m 120

5, 10, 15, 20m 120
5, 10, 15, 20m 120
Silica
Silica
Silica
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
325m2/g
325m2/g
325m2/g
15%
10%
Monomeric
Monomeric
Monomeric
Yes
L1
L7
L3
High Level of Resolution
GraceAlpha Columns
GraceAlpha offers resolution advantages due to its higher

mass transfer and optimized C18 and C8 bonding chemistry.
Packing
i.d. x Length
C18, 5m
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
C18, 10m
Crude 20-AA Synthetic Peptide

from Biopeptide Co., Inc.
C18, 15m
Adsorbence
C8, 5m
1.0
C8, 10m
Better resolution
of closely
eluting impurities
0.5
C8, 15m
Silica, 5m
GraceAlpha Column 5m
0
0
10
15
19.99 Min.
Silica, 10m
Adsorbence
Silica, 15m
1.0
0.5
Competitor 4m
0
0
Columns:
Mobile Phase:
Gradient:

Flow Rate:
10
15
19.99 Min.
All C18 4.6 x 250mm

A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile
Time: 0 20
%B: 20 40
1.0mL/min
The target peptide is better resolved from impurities, with 33% higher
recovery, on the GraceAlpha column.
52
more applications

Part No.
5140743
5140702
5140744
5140701
5141058
5141059
5140740
5140704
5140742
5140703
5141055
5141057
5140740
5140704
5140742
5140703
5141055
5141057
analytical hplc
Alltech Brava Columns
best
value
If You Like Hypersil BDS, Try This Lower-Cost Alternative

BDS phases for acids, bases, and neutrals
ODS phases for non-polars and lipophilics
Brava Phase Specifications

Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
C18 BDS
C18 ODS
C8
C8 BDS
Phenyl
Cyano
Cyano BDS
Amino (NH2)
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
3, 5m
3, 5m
3, 5m
3, 5m
5m
5m
5m
5m
5m
145
130
130
145
130
130
145
130
130
185m2/g
195m2/g
195m2/g
185m2/g
195m2/g
195m2/g
185m2/g
195m2/g
195m2/g
8.5%
8.5%
6%
5.5%
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Yes
Yes
Yes
Yes
No
No
No
No
No
L1
L1
L7
L7
L11
L10
L10
L8
L3
Brava HPLC Columns

Packing
i.d. x Length
Part No.
Waters
Fittings
Part No.
C18 BDS, 3m
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
50910
50912
50914
50916
50957
50961
50965
50969
50918
50920
50922
50924
50939
50945
50949
50953
50978
50981
50926
50928
50973
50976
50930
50933
50935
50937
50911
50913
50915
50917
50959
50963
50967
50971
50919
50921
50923
50925
50943
50947
50951
50955
50979
50983
50927
50929
50975
50977
50931
50934
50936
50938
Qty.
Part No.
3
3
3
3
3
3
3
3
3
ea
96472
96477
96473
96478
96480
96474
96479
96475
96476
80101
C18 BDS, 5m
C18 ODS, 3m
C18 ODS, 5m
C8 BDS, 3m
C8 BDS, 5m
C8, 3m
C8, 5m
Phenyl, 5m
Cyano BDS, 5m
Cyano, 5m
Amino, 5m
Silica, 5m
Metal Chelator
Medium-sized pores are ideal for resolution of small- to medium-sized compounds,

while its less-retentive nature means less mobile phase consumption.
10473
1. Pyridine
2. 2, 2 Dipyridyl
4 Min.
Column:
rava C18 BDS, 5m, 4.6 x 150mm

B
(Part No. 50914)
Mobile Phase: Acetonitrile:50mM KH2PO4 pH 3 (50:50)
Flow Rate:
1.0mL/min
Detector:
UV at 254nm
Brava All-Guard Cartridges*

Packing
i.d. x Length
C18 BDS, 5m
4.6 x 7.5mm
C18 ODS, 5m
4.6 x 7.5mm
C8 BDS, 5m
4.6 x 7.5mm
C8, 5m
4.6 x 7.5mm
Phenyl, 5m
4.6 x 7.5mm
Cyano BDS, 5m
4.6 x 7.5mm
Cyano, 5m
4.6 x 7.5mm
Amino, 5m
4.6 x 7.5mm
Silica, 5m
4.6 x 7.5mm
more applications

53
analytical hplc
Alltech Alltima Introduction
best
seller
High-Quality General-Purpose HPLC Columns
Extensive collection of published applications

Well suited for microbore applications, stable in popular microbore mobile phases
Acid and base deactivated symmetrical peaks
Polymerically bonded and double-endcapped for long column lifetimes
Analyze acids, bases, and neutrals in one run
Full range of column dimensions, from microbore to preparative
Alltima Phase Specifications

Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
C18
C18 LL
C8
Phenyl
Cyano
Amino (NH2)
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
3, 5, 10m
5m
3, 5, 10m
3, 5m
3, 5m
3, 5m
3, 5, 10m
100
100
100
100
100
100
100
340m2/g
340m2/g
340m2/g
340m2/g
340m2/g
340m2/g
340m2/g
16%
9%
9%
7.5%
Polymeric
Polymeric
Polymeric
Polymeric
Polymeric
Polymeric
Yes
Yes
Yes
Yes
Yes
No
L1
L1
L7
L11
L10
L8
L3
Basic Drugs
Antibacterials
8193
2
1
8764
1. Procainamide
2. Acetaminophen
3. Theophylline
4. Caffeine
1. Oxytetracycline
Dihydrate
2. Tetracycline HCL
3. Meclocycline
Sulfosalicylate Salt
3
4
0
0
10
Column:
Mobile Phase:
Flow Rate:
Detector:
15 Min.
Column:
Mobile Phase:
Gradient:

Flow Rate:
Detector:
Alltima C8, 5m, 4.6 x 150mm

Methanol:50mM KH2PO4, pH 3.0 (20:80)
1.0mL/min
UV at 254nm
Antihistamines
8887
1.
2.
3.
4.
5.
6.
Column:
Mobile Phase:
Gradient:

Flow Rate:
Detector:
54
Naringin
Neohesperidin
Myricetin
Quercetin
Naringenin
Kaempferol

A: 50mM KH2PO4, pH 2.5 B: CH3CN
Time: 0 15
%B: 20 40
1.0mL/min
UV at 280nm
1.
2.
3.
4.
5.
5 6
10
7232
10 Min.

Time: 0 10
%B: 15 40
1.0mL/min
UV at 254nm
Natural Products
15 Mi
Column:
Mobile Phase:
Gradient:

Flow Rate:
Detector:
10
15
20
25 Mi

Time: 0 5 20
%B: 25 25 70
1.3mL/min
UV at 220nm
Tripelennamine
Triprolidine
Cyclizine
Chlorcyclizine
Meclizine
analytical hplc
Alltech Alltima Reversed-Phase Columns

Taxol and Related Taxanes in Yew
(taxus brevifolia)
2
4,5 6
3
Column:
Mobile Phase:
Flow Rate:
Detector:
8 9 1011
12
13
9221
10-deacetylbaccatin III
Baccatin III
7-xylosyl-10-deacetyltaxol B
Taxinine M
7-xylosyl-10-deacetyltaxol
7-xylosyl-10-deacetyltaxol C
10-deacetyltaxol
7-xylosyltaxol
Cephalomannine (Taxol B)
7-epi-10-deacetyltaxol
Taxol
Taxol C
7-epitaxol
8 Min.
Alltima Phenyl Rocket, 3m, 7 x 53mm

0.05% TFA in H2O:MeOH:CH3CN (50:19:31)
2.5mL/min
UV at 230nm
Anticonvulsants
7233
5
1.
2.
3.
4.
5.
6.
6
123
4
Column:
Mobile Phase:
Gradient:

Flow Rate:
Detector:
12
16
Ethotoin
Phenobarbital
Methsuximide
Mephenytoin
Carbamazepine
Diazepam
20 Mi
Alltima Phenyl, 5m, 4.6 x 250mm

A: H2O B: CH3CN
Time: 0 20 25 30
%B: 15 60 60 15
1.5mL/min
UV at 254nm
Alltima All-Guard Cartridges*

Packing
i.d. x Length
Qty.
Part No.
C18, 5m
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
2.1 x 7.5mm
3.0 x 7.5mm
4.6 x 7.5mm
3
3
3
3
3
3
3
3
3
3
3
3
ea
96680
96361
96080
96432
96433
96285
96441
96362
96081
96442
96445
96082
80101
C18-LL, 5m
C8, 5m
Phenyl, 5m

Packing
Format
i.d. x Length
Microbore
Microbore
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Solvent Reducer
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Rocket
Rocket
C18, 5m
Microbore
Microbore
Microbore
Microbore
Solvent Reducer
Solvent Reducer
Analytical
Analytical
C18, 10m
Analytical
C18-LL, 5m Microbore
Microbore
Microbore
Microbore
Solvent Reducer
Solvent Reducer
Analytical
Analytical
C8, 3m
Analytical
Analytical
Analytical
Rocket
Rocket
C8, 5m
Microbore
Microbore
Microbore
Microbore
Solvent Reducer
Analytical
Analytical
C8, 10m
Analytical
Phenyl, 3m Microbore
Microbore
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Phenyl, 5m Solvent Reducer
Analytical
Analytical
C18, 3m
1.0 x 100mm
1.0 x 150mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
3.0 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
1.0 x 150mm
1.0 x 250mm
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
1.0 x 150mm
1.0 x 250mm
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
1.0 x 150mm
1.0 x 250mm
2.1 x 150mm
2.1 x 250mm
3.0 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
1.0 x 100mm
1.0 x 150mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
3.0 x 150mm
4.6 x 150mm
4.6 x 250mm
Standard
Part No.
Waters
Fittings
Part No.
43853
43864
43836
43803
43849
43821
43852
81143
43837
43851
81412
81382
81387
50603
50605
88384
88385
88370
88371
81140
81142
88052
88056
88307
88390
88391
88388
88389
81144
81145
88069
88099
81413
81392
81397
50607
50609
88394
88395
88372
88373
81146
88072
88076
88317
43892
43854
43809
43865
43835
43866
43813
43883
43822
81178
81181
81188
81147
88087
88092
81383
81388
88053
88057
88308
88082
88235
81393
81398
88073
88077
88318
88184
81191
88088
88093
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
Alltima HPLC Reversed-Phase Columns
55
analytical hplc
Alltech Alltima C18 Validation Kits
Alltima columns have the batch-to-batch and column-tocolumn reproducibility required for method development. Each
kit includes three columns made from three different batches
of Alltima C18.
Alltima is Stable and Reproducible
Initial Separation
7553
Alltima C18 Validation Kits

Description
Includes:
i.d. x Length
Part No.
C18, 3m
C18, 5m
C18, 5m
3 columns from 3 different batches

4.6 x 100mm
4.6 x 150mm
4.6 x 250mm
11350
11541
11543
2
1
45
Alltech Alltima
Normal-Phase Columns
Alltima HPLC Normal-Phase Columns
Packing
Format
i.d. x Length
Cyano, 3m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Microbore
Microbore
Expedite MS
Expedite MS
Microbore
Microbore
Microbore
Expedite MS
Expedite MS
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
1.0 x 100mm
1.0 x 150mm
2.1 x 10mm
2.1 x 20mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
4.6 x 10mm
4.6 x 20mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
Cyano, 5m
Amino, 3m
Amino, 5m
Silica, 3m
Silica, 5m
Silica, 10m
Standard
Part No.
Waters
Fittings
Part No.
81179
81182
81189
88180
88189
81180
81183
81190
88205
88217
43824
43855
43891
43801
43876
43823
43884
43889
43886
81414
81404
81409
88123
88171
88327
81186
81192
88182
88191
81187
81193
88207
88218
81405
81410
88124
88172
88328
Alltima All-Guard Cartridges*

Packing
i.d. x Length
Qty.
Part No.
Cyano, 5m
Amino, 5m
Silica, 5m
4.6 x 7.5mm
4.6 x 7.5mm
2.1 x 7.5mm
4.6 x 7.5mm
3
3
3
3
ea
96084
96085
96450
96083
80101

5 10 15 20 25 30 35 40 45 50 55 Min.
After 500 Hours of Washing at 60C

7554
2
1
45
5 10 15 20 25 30 35 40 45 50 55 Min.
1. Gly-Tyr
2. Val-Tyr-Val
3. Met-Enkephalin
4. Leu-Enkephalin
5. Angiotensin II
Column:
Mobile Phase:

Gradient:

Flow Rate:
Detector:

A: Water:Acetonitrile:TFA (95:5:0.1)
B: Water:Acetonitrile:TFA (25:75:0.1)
Time: 0 60
% B: 5 30
1mL/min
UV at 220nm
Column capacity and selectivity remained constant after washing an

Alltima C18 column for 500 hours with 0.5% TFA:Acetonitrile (50:50)
at 60C.
tech tip

columns for low volume and microbore systems. See page 31 for
more explanation of the Rocket and Expedite formats.
56
analytical hplc
Alltech Apollo Columns
best
value
Economical, High Performance Columns

Easy scale-up from analytical to prep
Extended pH stability1.5 to 10.5
Apollo Phase Specifications

Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
C18
C8
Phenyl
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
5m
5m
5m
5m
100
100
100
100
340m2/g
340m2/g
340m2/g
340m2/g
15%
9%
8%
Monomeric
Monomeric
Monomeric
Yes
Yes
Yes
L1
L7
L11
L3
Qty.
Part No.
3
3
3
3
ea
96454
96463
96430
96419
80101
Apollo HPLC Columns
Apollo All-Guard Cartridges*
Packing
Format
i.d. x Length
Part No.
Packing
C18, 5m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
36505
36511
36506
36512
36538
36544
36507
36513
C18, 5m
4.6 x 7.5mm
C8, 5m
4.6 x 7.5mm
Phenyl, 5m
4.6 x 7.5mm
Silica, 5m
4.6 x 7.5mm
C8, 5m
Phenyl, 5m
Silica, 5m
i.d. x Length
Batch-tested Apollo HPLC columns use high purity, basedeactivated silica for powerful separations at an economical
price. They are ideal for educational labs or for method
development.
Alltech Econosphere Columns

Low-cost columns for common chromatography applications
Acid-washed for low metal content
Easy scale-up from analytical to prep
Econosphere Phase Specifications
Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
C18
C8
Cyano
Amino (NH2)
Silica
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
3, 5, 10m
3, 5, 10m
5m
5m
3, 5, 10m
80
80
80
80
80
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
10%
5%
Monomeric
Monomeric
Monomeric
Polymeric
Yes
Yes
Yes
Yes
No
L1
L7
L10
L8
L3
Econosphere HPLC Columns (continued)
Econosphere HPLC Columns

Packing
Format
i.d. x Length
C18, 3m
Analytical
Analytical
Analytical
Rocket
Rocket
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
4.6 x 150mm
4.6 x 250mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
C18, 5m
C8, 3m
C8, 5m
Cyano, 5m
Standard
Part No.
Waters
Part No.
70153
70154
70155
50673
50675
70065
70066
70165
70166
70167
70085
70086
70025
70026
70156
70157
70158
70070
70071
70168
70169
70170
70090
70030
70031
Packing
Format
i.d. x Length
Amino, 5m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 150mm
4.6 x 250mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
Silica, 3m
Silica, 5m
Standard
Part No.
Waters
Part No.
70045
70046
70177
70178
70179
70005
70006
70050
70051
70180
70181
70182
70010
70011
Econosphere All-Guard Cartridges*

Packing
i.d. x Length
C18
4.6 x 7.5mm
C8
4.6 x 7.5mm
Cyano
4.6 x 7.5mm
Amino
4.6 x 7.5mm
Silica
4.6 x 7.5mm
Qty.
Part No.
3
3
3
3
3
ea
96121
96122
96123
96124
96125
80101
*Guard holder required.
57
analytical hplc
Alltech Adsorbosphere Columns

Reliable performance from rugged, reproducible phases
High temperature bonding for exceptional surface coverage, stability, and reproducibility.
Adsorbosphere columns are the workhorses of any chromatography lab. Choose from
four varieties:
Adsorbosphere has a moderate carbon load for common applications.
Adsorbosphere HS has a larger surface area and higher carbon load for stronger retention of hydrophobic compounds.
Adsorbosphere UHS has the largest surface area and highest carbon load for the highest resolution of hydrophobic
compounds.
Adsorbosphere XL is a lower-cost, larger pore alternative. C18-B separates strongly basic analytes more efficiently then
standard C18.
Adsorbosphere Phase Specifications
Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
Adsorbosphere C18
Adsorbosphere C18 HS
Adsorbosphere C18 UHS
Adsorbosphere C8
Adsorbosphere Phenyl
Adsorbosphere Cyano
Adsorbosphere Cyano-AQ
Adsorbosphere Amino (NH2)
Adsorbosphere Silica
Adsorbosphere SAX
Adsorbosphere SCX
Adsorbosphere XL C18
Adsorbosphere XL C18-B
Adsorbosphere XL C8
Adsorbosphere XL C1 (TMS)
Adsorbosphere XL Silica
Adsorbosphere XL SAX
Adsorbosphere XL SCX
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
3, 5, 10m
3, 5m
5, 10m
5m
5m
5m
5m
3, 5m
5m
5m
5m
3, 5m
5m
3, 5m
5m
5m
5, 10m
5, 10m
80
60
60
80
80
80
120
80
80
80
80
90
90
90
90
90
90
90
200m2/g
350m2/g
500m2/g
200m2/g
200m2/g
200m2/g
170m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
200m2/g
12%
20%
30%
8%
5%
11%
12%
6%
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Polymeric
Polymeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Yes
Yes
Yes
Yes
Yes
Yes
No
No
Yes
Yes
Yes
Yes
Yes
Yes
Yes
L1
L1
L1
L7
L11
L10
L10
L8
L3
L1
L1
L7
L13
L3
Adsorbosphere HPLC Columns
Vitamins D2 & D3
5401
1
2
1. Vitamin D2
2. Vitamin D3
Packing
Format
i.d. x Length
C18, 3m
Analytical
Rocket
Rocket
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 100mm
7 x 33mm
7 x 53mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
287092
50623
50625
287072
287062
287052
287122
287112
287532
287542
287202
50005
500050
287282
287242
287012
287512
287472
287093
287073
287063
287053
287123
287113
287533
287543
287203
50035
500060
287283
287243
287013
287513
287473
Standard
Part No.
Waters
Fittings
Part No.
28780
28786
28817
28821
28781
28787
28818
28822
C18, 5m
C18, 10m
C8, 5m
Phenyl, 5m
Cyano, 5m
Cyano-AQ, 5m
10
20
Column:
30 Min.
dsorbosphere UHS C18, 5m, 4.6 x 150mm

A
(Part No. 288118)
Mobile Phase: 80% Acetonitrile 20% Methanol
Flow Rate:
1.5mL/min
Detector:
UV at 254nm
Column Temp: Ambient
Amino, 3m
Amino, 5m
Silica, 5m
SAX, 5m
SCX, 5m
Adsorbosphere HS HPLC Columns

Packing
Format
i.d. x Length
C18 HS, 3m
Analytical
Analytical
Analytical
Analytical
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
C18 HS, 5m
58
Waters
Fittings
Part No.
Standard
Part No.
analytical hplc
Alltech Adsorbosphere
Columns (continued)
Alltech Allsphere Columns
Adsorbosphere UHS HPLC Columns

Format
i.d. x Length
C18 UHS, 5m
C18 UHS, 10m
Analytical
Analytical
4.6 x 150mm
4.6 x 250mm
288118
288133
Waters
Fittings
Part No.
288119
288134
Adsorbosphere XL HPLC Columns

Packing
Format
i.d. x Length
XL C18, 3m
Analytical
Analytical
Rocket
Rocket
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 100mm
4.6 x 150mm
7 x 33mm
7 x 53mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
XL C18, 5m
XL C18-B, 5m
XL C8, 3m
XL C8, 5m
XL C1, 5m
XL Silica, 5m
XL SAX, 5m
XL SAX, 10m
XL SCX, 5m
XL SCX, 10m
Standard
Part No.
Waters
Fittings
Part No.
20237
20381
50633
50635
20390
20552
20548
20247
20320
20676
20383
20386
20628
20365
20256
20538
20550
20314
20525
20545
20719
20286
20394
20462
20579
20344
20627
20203
20607
20460
20452
20636
20398
20654
20318
20561
20303
20531
20564
20684
Adsorbosphere All-Guard Cartridges*

Packing
i.d.x Length
Amino, 5m
4.6 x 7.5mm
C18, 5m
4.6 x 7.5mm
C18 HS, 5m
4.6 x 7.5mm
C18 UHS, 5m
4.6 x 7.5mm
C8, 5m
4.6 x 7.5mm
Cyano, 5m
4.6 x 7.5mm
Cyano-AQ, 5m
4.6 x 7.5mm
Phenyl, 5m
4.6 x 7.5mm
Silica, 5m
4.6 x 7.5mm
SAX, 5m
4.6 x 7.5mm
SCX, 5m
4.6 x 7.5mm
XL C18, 5m
4.6 x 7.5mm
XL C18-B, 5m
4.6 x 7.5mm
XL C8, 5m
4.6 x 7.5mm
XL C1, 5m
4.6 x 7.5mm
XL Silica, 5m
4.6 x 7.5mm
XL SAX, 5m
4.6 x 7.5mm
XL SCX, 5m
4.6 x 7.5mm
Allsphere HPLC Columns

Packing
i.d. x Length
Standard
Part No.
ODS-1, 5m
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
778441
778364
778487
778558
778545
778736
778561
778609
778571
778744
778211
778223
778689
778752
778713
778361
778739
778371
778382
778554
778389
778376
778765
778188
ODS-2, 3m
ODS-2, 5m
C8, 3m
C8, 5m
C6, 5m
Phenyl, 5m
Cyano, 5m
Amino, 5m
Silica, 3m
Silica, 5m
SAX, 5m
SCX, 5m
Waters
Fittings
Part No.
778443
778357
778489
778560
778547
778738
778563
778611
778573
778746
778217
778224
778691
778754
778715
778362
778741
778372
778383
778556
778391
778377
778767
778189
Packing
Standard
Part No.
Try Alltechs lower cost Allsphere columns as a replacement

for Spherisorb columns. If you are not satisfied with
Allsphere as a direct replacement for your Spherisorb
applications, we will refund your money.
Allsphere All-Guard Cartridges*

Qty.
Part No.
Packing
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
ea
96044
96041
96079
82181
96042
96047
96048
96045
96046
96049
96075
96088
96089
96090
96093
96097
96098
96099
80101
ODS-1, 5m
4.6 x 7.5mm
ODS-2, 5m
4.6 x 7.5mm
C8, 5m
4.6 x 7.5mm
C6, 5m
4.6 x 7.5mm
Phenyl, 5m
4.6 x 7.5mm
Cyano, 5m
4.6 x 7.5mm
Amino, 5m
4.6 x 7.5mm
Silica, 5m
4.6 x 7.5mm
SAX, 5m
4.6 x 7.5mm
SCX, 5m
4.6 x 7.5mm
i.d. x Length
Qty.
Part No.
3
3
3
3
3
3
3
3
3
3
ea
96402
96403
96404
96405
96406
96408
96409
96401
96410
96411
80101
more info
Looking for Adsorbosil columns?

Please visit www.discoverysciences.com.
59
analytical hplc
Grom Sil Columns
High-Quality, Wide Variety, HPLC Columns for All Types of Applications

Highly consistent manufacturing for excellent reproducibility
40 different phases to choose from, general purpose to specialty phases
Wide range of particle sizes from sub 2m to 10m
Available in nano to preparative column formats
High purity spherical silica with high and universal selectivity for broad range of applications. Different phases and bonding
technologies give you the power and flexibility
ST = standard bonding with endcapping high-quality universal column
NE = not endcapped high polar selectivity
HE = hydrophilic endcapping increased polar selectivity; pH -stable at high pH, high carbon load and very low silanol activity
CP = possiblility to work at high pH
FE = Fully endcapped maximum silica coverage
PA = Cross-linked polyamino better stability
pH = pH stable use mobile phases up to pH 11
Grom Sil Phase Specifications
Phase
Base
Particle
Material Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
EndUSP
capped? L-code
ODS-3 CP (encapsulated)
ODS-4 HE (hydrophilic endcapping)
ODS-5 ST (standard)
ODS-7 pH (pH-stable)
Octyl-5 CP (encapsulated)
Phenyl-1 FE (fully endcapped)
Phenyl-2 CP (encapsulated)
Cyano-3 CP (encapsulated)
Amino-2 PA (Crs linked Poly-NH)
Diol
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
3, 5, 7, 10m
3, 4, 5, 7, 10m
3, 4, 5, 7, 10m
4m
3, 5, 7, 10m
3, 5, 10m
5m
5m
5m
5, 10m
120, 300
120, 200
60, 120, 200
80
120, 300
120, 300
120, 300
120
120
60, 120, 200
320, 170m2/g
300, 200m2/g
580, 300, 200m2/g
510m2/g
320, 170m2/g
300, 150m2/g
320, 170m2/g
320m2/g
300m2/g
580, 300, 200m2/g
15, 6%
16, 11%
22, 17, 12%
22%
10, 5.5%
9, 5%
7, 4%
4%
Polymeric
Monomeric
Monomeric
Polymeric
Polymeric
Monomeric
Polymeric
Polymeric
Polymeric
Monomeric
No
Yes
Yes
No
No
Yes
No
No
No
No
Spherical
Spherical
Spherical
Irregular
Spherical
Spherical
Irregular
Spherical
Spherical
Spherical
Color AdditivesDyes
Nucleosides and Bases

1 4 5+6
23
4
10
Column:
Flow Rate:
Mobile Phase:

Gradient:
Column Temp:
Detector:
60
20
30
10
11
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
Tartrazine
Amaranth
Indigo Carmine
New Coccine
Sunset Yellow
Fast Green
Brilliant Blue
Erythosine
Acid Red
Phloxine
Ros Bengale
40 Min.
Grom Sil 120 ODS-5 ST, 5m, 4 x 150mm

0.8mL/min
A: 10mM NH4-phosphate, pH 6.0:MeOH (90:10)
B: 10mM NH4-phosphate, pH 6.0:MeOH (20:30)
0100% B (040min, linear), 100% B (4060min)
30C
UV at 254nm
3 7+8
9
2
10 1112
1314
17
15 18
16
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
Cytosine
Cytidine
Uracil
Deoxycytidine
Guanine
Adenine
Hypoxanthine
Uridine
Xanthine
Thymine
Inosine
Guanosine
Deoxyinosine
Deoxyguanosine
Xanthosine
Thymidine
Adenosine
Deoxyadenosine
0 5 10 15 20 25 30 35 Min.
Column:
Flow Rate:
Mobile Phase:

Gradient:

Detector:
Grom Sil 120 ODS-5 ST, 5m, 4 x 250mm

0.5mL/min
A: 20mM NH4-acetate, pH 3.5
B: 20mM NH4-acetate, pH 3.5:MeOH (90:10)
Time: 0 5 13 40
%B: 30 30 100 100
UV at 260nm, 0.08 AUFS
L1
L1
L1
L1
L7
L11
L11
L8
L20
analytical hplc
Grom Sil Columns

Grom Sil Columns
Water-soluble Vitamins
5
Format
ODS-3 CP, 3m
Microbore
Microbore
Microbore
Solvent Reducer
Solvent Reducer
Solvent Reducer
Analytical
Analytical
Solvent Reducer
Analytical
Analytical
Analytical
Capillary
Microbore
Microbore
Microbore
Microbore
Microbore
Analytical
Analytical
Analytical
Analytical
Capillary
Capillary
Capillary
Microbore
Microbore
Solvent Reducer
Analytical
Analytical
Analytical
Microbore
Microbore
Microbore
Analytical
Analytical
Analytical
Analytical
Capillary
7
3
8
2
4
10
15
20
1.
2.
3.
4.
5.

6.
7.

8.
L-Ascorbic Acid (Vit. C)

Nicotinic Acid
Pyridoxine HCl (Vit. B6)
Nicotinamide
Thiamine Hydrochloride
(Vit. B1)
Folic Acid
Cyanocobalamin
(Vit. B12)
Riboflavin
ODS-3 CP, 5m
ODS-4 HE, 10m

ODS-4 HE, 3m
25 Min.
Column:
rom Sil 120 ODS-5 ST, 5m, 4 x 150mm

G
(Part No. 5113642)
Flow Rate:
0.8mL/min
Mobile Phase: A: 20mM K-phosphate, pH 6.6

B: 20mM K-phosphate, pH 6.6:ACN (80:20)
Gradient:
Time: 0 5 20 25

%B: 0 0 100 100
Column Temp: 30C
Detector:
UV at 254nm
ODS-4 HE, 5m
Fat-soluble Vitamins
ODS-5 ST, 3m
ODS-5 ST, 5m
23
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
1
4
6
5
7
10
8
9
Menadione (Vit. K3)

Retinol (Vit. A)
Retinol Acetate
Menaquinone (Vit. K2)
d-Tocopherol
Ergocalciferol (Vit. D2)
Cholecalciferol (Vit. D3),
a-Tocopherol (Vit. E),
a-Tocopherol Acetate,
Phylloquinone (Vit. K1)
ODS-7 pH, 4m
Octyl-5 HE, 3m
Octyl-5 CP, 5m
Phenyl-1 FE, 5m
10
15
20
25 Min.
Column:
rom Sil 120 ODS-5 ST, 5m, 4 x 150mm

G
(Part No. 5113642)
Flow Rate:
0.8mL/min
Mobile Phase: Acetonitrile
Column Temp: 30C
Detector:
UV at 280nm
more applications

Phenyl-2 CP, 5m
Cyano-3 CP, 5m
Amino-2 PA, 5m
Diol, 5m
i.d. x Length
1 x 60mm
2 x 125mm
2 x 250mm
3 x 60mm
3 x 100mm
3 x 150mm
4 x 60mm
4.6 x 150mm
3 x 125mm
4 x 125mm
4.6 x 250mm
4.6 x 250mm
0.3 x 150mm
1 x 50mm
1 x 150mm
2 x 100mm
2 x 125mm
2 x 150mm
4 x 50mm
4 x 60mm
4 x 125mm
4.6 x 125mm
0.1 x 150mm
0.2 x 250mm
0.5 x 250mm
2 x 125mm
2 x 150mm
3 x 150mm
4 x 125mm
4 x 250mm
4.6 x 150mm
2 x 60 mm
2 x 150mm
2 x 250mm
4 x 125mm
4 x 150mm
4 x 250mm
4.6 x 250mm
0.3 x 250mm +
10mm Guard Column
Microbore
1 x 50mm
Microbore
2 x 40mm
Solvent Reducer 3 x 150mm
Analytical
4.6 x 125mm
Analytical
4.6 x 150mm
Analytical
4.6 x 125mm
Microbore
2 x 250mm
Analytical
4 x 150mm
Microbore
2 x 100mm
Analytical
4 x 250mm
Analytical
4 x 250mm
Microbore
2 x 125mm
Analytical
4.6 x 60mm
Microbore
2 x 250mm
Analytical
4 x 250mm
Analytical
4.6 x 250mm
Microbore
2 x 100mm
Part No.
5115722
5115672
5117056
5113597
5117398
5113602
5113598
5117003
5113604
5115479
5113609
5116144
5118148
5116060
5113619
5115975
5116880
5114808
5118892
5117386
5113618
5117572
5117667
5119092
5118525
5113623
5116012
5117727
5115808
5113626
5116777
5117044
5118335
5116342
5116219
5113642
5113646
5113647
5119756
Packing
5118213
5115266
5116956
5113654
5115840
5113265
5117325
5117565
5113695
5113696
5116429
5117410
5116441
5115469
5113466
5115306
5117476
more info
For large molecule columns, refer to page 84 97.
61
analytical hplc
Grom Sapphire Column
High Purity for Optimal Performance of Challenging Separations

Excellent peak shapeHighly symmetrical peaks from high purity silica base
High speed and high capacityRange of pore sizes and surface area for improved speed and resolution
Optimized for consistent flowcritical in low volume applications
Variety of column formats1.5, 3, and 5m particles
Sapphire Phase Specifications
Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
C18
C8
C4*
Silica*
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
3, 5, 10m
3, 5, 10m
3, 5, 10m
3, 5, 10m
65, 110
65, 110
65, 110
65, 110
500, 270m2/g
500, 270m2/g
500, 270m2/g
500, 270m2/g
23, 16%
15, 10%
10.5, 7%
Monomeric
Monomeric
Monomeric
Yes
Yes
Yes
No
L1
L7
L26
L3
*Columns available upon request.
Separation of Antiepileptics
1.
2.
3.
4.
5.
6.
7.
8.
1
4
2
3
6
5
Stability of Grom Sapphire Packings (Daldrup)
Ethosuximide
Primidone
Lamotrigine
Phenobarbital
Carbamazepine-10,11-epoxide
IS, Hexobarbital
Phenytoin
Carbamazepine
78
1000 mV
2
13
1.
2.
3.
4.
5.
6.
500
Uracil
4-Hydroxybenzoic Acid
Diphenylhydramine
5-(p-Methylphenyl)-5-phenylhydantoin
Diazepam
Toluene
1000 Injection
500 Injection
Column:

Flow Rate:
Mobile Phase:
Detector:
4 Min.
Grom Sapphire 110 C18, 5m, 4 x 53mm

(Part No. 5117499)
1.5mL/min
50mM Na-phosphate, pH 6.5:MeOH:ACN (62:23:15)
UV at 204nm
Column:

Flow Rate:
Mobile Phase:
Column Temp:
Detector:
1 Injection
12 16 20 24 28 32 36 Min.
Grom Sapphire 110 C18, 5m, 4 x 125mm

(Part No. 5117505)
1mL/min
50mM Na-phosphate, pH 2.3:ACN (58:42)
RT
UV at 230nm
Sapphire HPLC Columns

Packing
i.d. x Length
C18, 3m
0.030 x 150mm
0.075 x 150mm
0.100 x 100mm
4 x 53mm
4 x 125mm
2 x 250mm
C18, 5m
C8, 5m
Part No.
5119303
5133239
5135280
5117499
5117505
5118934


related products
related products
Need high-pressure
polymeric fittings?
See pages 112114
for our full selection of
high-pressure fittings.
Looking for HPLC column

prefilters? See page 111.
6673
62
analytical hplc
Grom Amino Acid Column Kits

Amino Acid Analysis by Precolumn
Derivatization Using OPA/IBLC

Derivatization With OPA/3-MPA
Amino Acid Analysis after Precolumn Derivatization

with OPA/Mercaptopropionic AcidExtended Standard
OPA/IBLC Derivatized D- and L-Amino Acids

12 34
56
29 30
22 2526
21 23 28
14
9 12 15
7
8
13
10
11
Highest sensitivity (510fMol)

Short analysis time
Fully automated derivatization
Best resolution
19
18 20 24 27
16 17
15
9
13
11
4 67
12
14
8 10
3
5
16 17
20 21 22
18
19
24
23
0
1.
2.
3.
4.
5.
6.
7.
L-Asp
D-Asp
L-Glu
D-GLu
L-Ser
D-Ser
L-Thr
10
8.
9.
10.
11.
12.
13.
14.
Column:
Flow Rate:
Mobile Phase:

Gradient:
Column Temp:
Detector:
20
Gly
D-Thr
L-His
D-His
L-Ala
L-Arg
D-Arg
30
15.
16.
17.
18.
19.
20.

40
50
D-Ala
L-Tyr
D-Tyr
L-Val
L-Met
L-Nor-Val
(Int. Standard)
60
21.
22.
23.
24.
25.
26.
70 Min.
D-Met
D-Val
L-Phe
L-Ile
D-Phe
L-Leu
27.
28.
29.
30.
D-Ile
D-Leu
L-Lys
D-Lys
OPA/IBLC Kit
Complete kit includes 4.0mm i.d. x 250mmlength column,
OPA/IBLC derivitization reagents, and detailed working
instructions.
987.0000
Gin
Gly
Thr
His
20
9.
10.
11.
12.
Cit
m-His
Ala
Tau
30
13.
14.
15.
16.
Arg
AAB
Tyr
Val
40 Min.
17.
18.
19.
20.
Met
Nor-Val
Trp
Ile
21.
22.
23.
24.
Phe
Leu
Orn
Lys
Grom Sil OPA-1, 4 x 250mm

A: 25mM Na-phosphate, pH 7.2:THF (995:5)
B: 25mM Na-phosphate, pH 7.2:MeOH/ACN (50:35:15)
Time: 0 3 20 25 36 40 43
%B: 0 0 35 50 60 70 100
0.8mL/min
25C
Fluorescence 330nm(exc.), 450nm(em.)
Description
Part No.
Complete kit includes 4.6mm i.d. x 150mm length column,

OPA/3-MPA derivitization reagents, and detailed working
instructions.
980.0000
Separation of a D-, L-Amino Acid Standard by HPLC

Employing the FLEC-ADAM Approach
High resolution and high sensitivity

Rapid, automated or manual derivatization
Primary and secondary amines
Stable derivatives
FLEC/ADAM Kit
Complete kit includes 4.0mm i.d. x 250mm length column,
FLEC/ADAM derivitization reagents, and detailed working
instructions.
10
5.
6.
7.
8.
OPA/3-MPA Kit
Part No.

Derivatization Using FLEC/ADAM
Description
Asp
Glu
Asn
Ser
Column:
Mobile Phase:

Gradient:

Flow Rate:
Column Temp:
Detector:
Grom Sil OPA-2, 4 x 250mm

0.8mL/min
A: 23mM Na-acetate, pH 6.0
B: 600mL MeOH + 50mL ACN
053% B (075min)
24C
Description
0
1.
2.
3.
4.
High resolution (both optical antipodes of all protein

amino acids in a single chromatogram)
Outstandingly high sensitivity (fMol)
Extraordinarily long lifetimes of columns
Part No.
984.0000
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
L-Arg
D-Arg
L-Ser
D-Ser
D-Asp
L-Asp
D-Thr
L-Thr
D-Glu
L-Glu
D,L-Pro
D-Ala
L-Ala
FLEC-OH
D-Tyr
D-Met
11
20
17.
18.
19.
20.
L-Tyr
L-Met
D-Val
D-Phe
Column:
Flow Rate:
Mobile Phase:
Gradient:

Column Temp:
Detector:
2 3 4 78 9
10
56
30
21.
22.
23.
24.
12
13
40
14
26
29
20 22
18 21 25 27 31
32
16 19
28
24
33
23
30
17
15
50
60
70 Min.
L-Val
25. D-Leu
28. D-His 32. L-Om
L-Phe
26. L-allo-lie; 29. D-Om 33. L-Lys
D-allo-lie
L-lie
30. L-His
D-lle
27. L-Leu
31. D-Lys
Grom Sil FLEC-1, 4 x 250mm

0.75mL/min
A: 50mM Na-acetate, pH 4.0 B: ACN C: THF
Time: 0 8 22 70
%B: 17 17 30 50
%C: 8 8 0 0
40C
63
analytical hplc
Jones Genesis Columns
Genesis columns use a new generation adsorbent based

on high-purity metal-free 120 spherical silica. Particle size
offering includes 3, 4, 7, and 15m. Genesis columns exhibit
excellent peak symmetry and exceptional pH stability from
1 to 10.
Genesis Phase Specifications
Phase
Base Material
Particle Shape Particle Size
Pore Size
Surface Area
Carbon Load Phase Type
Endcapped? USP L-code
C18
AQ
C8
C8e/c
C4
Phe
CN
Amino (NH2)
Carbohydrate
CN-TCA
Petro-XP
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
120
120
120
120
120
120
120
120
120
120
120
120
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
300m2/g
18%
15%
11%
11%
6.3%
9.4%
7%
3.5%
7%
n/a
Yes
Yes
No
Yes
Yes
Yes
Yes
No
Yes
n/a
3, 4, 7, 15m
4, 7m
3, 4, 7, 15m
3, 4, 7, 15m
4m
4m
3, 4m
3, 4m
4m
4m
4m
3, 4, 7, 15m
Genesis C18 Reversed Phase
Genesis C18 bonding is monomeric. A unique proprietary

end-capping reagent, which is less prone to acid hydrolysis
than trimethylsilane, provides freedom from residual silanols
and enhanced stability under low-pH operating conditions.
Genesis C18 columns also exhibit superior stability at
alkaline pH. The permissible operating range is pH 110.
Inertness Test
1.
2.
3.
4.
Uracil
Pyridine
2,3-Dimethylphenol
Methylbenzoate
Designed for separating hydrophilic and polar

compounds
Stable retention times in 100% aqueous mobile phases
Rapid equilibration
Unique reversed-phase selectivity
The Genesis AQ adsorbent employs an optimum ratio of
C18, short (non-TMS) chains, and polar surface groups
bonded to high-purity 120 silica to allow rapid equilibration
and provide consistent, reproducible chromatography with
stable retention times in 100% aqueous eluents.
Retention on Genesis AQ is greater for polar analytes but
lower for non-polar compounds compared to Genesis C18.
Uracil, which is typically an unretained peak on C18 columns,
is well retained on the Genesis AQ adsorbent. Acids and
bases exhibit good peak shapes. Although Genesis AQ
excels with water-rich mobile phases, it can also be used in
gradient and isocratic modes with a full spectrum of mobile
phases.
Nucleotide 5'-Monophosphates
2
5
3
Column:
Flow Rate:
Mobile Phase:
Detector:
5 Mi
enesis FM15960E C18, 4m, 4.6 x 150mm

G
0.8mL/min
Isocratic, 50% ACN in water
UV at 254nm
Column:
Flow Rate:
Mobile Phase:
Detector:
Column Temp:
1.
2.
3.
4.
5.
KNO3
CMP
UMP
GMP
AMP
1
0
64
L1
L1
L7
L7
L26
L11
L10
L8
L3
Genesis AQ Reversed Phase
Excellent peak symmetry

Exceptional stability from pH 1 to 10
Reduced need for mobile-phase modifiers
Long column life
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Polymeric
Monomeric
Monomeric
Monomeric
n/a
10
12
14 Mi
enesis FM15951E AQ, 4m, 4.6 x 150mm

G
1.0mL/min
Isocratic, 50mM NH4OAc, pH 5.5
UV at 254nm
30C
analytical hplc
Jones Genesis Columns

Genesis C8(EC) Reversed Phase
Genesis C8 Reversed Phase

Non-endcapped
Suitable for lower pH separations
Genesis C8 bonding is monomeric. They are suitable
for separations under lower pH conditions and may offer
selectivity advantages for some samples.
C8(EC) is double-bonded before fully endcapped using a

unique proprietary end-capping reagent. It is less prone to
acid hydrolysis than trimethylsilane, which provides freedom
from residual silanols and enhanced stability under low-pH
operating conditions. The bonding is monomeric. Genesis
C8(EC) columns exhibit superior stability at alkaline pH.
Genesis Phenyl
Unique reversed-phase chemistry
Improve the chromatography of polar aromatic, fatty
acids, and basic pharmaceuticals
Genesis Phenyl columns provide impressive peak symmetry
for both acidic and basic compounds.
Genesis Columns
Length:
Genesis AQ
4m
1.0mm i.d.
2.1mm i.d.
3.0mm i.d.
4.0mm i.d.
4.6mm i.d.
Genesis C18
3m
1.0mm i.d.
2.1mm i.d.
3.0mm i.d.
4.0mm i.d.
4.6mm i.d.
4m
1.0mm i.d.
2.1mm i.d.
3.0mm i.d.
4.0mm i.d.
4.6mm i.d.
Genesis C8
3m
1.0mm i.d.
2.1mm i.d.
3.0mm i.d.
4.0mm i.d.
4m
1.0mm i.d.
2.1mm i.d.
3.0mm i.d.
4.0mm i.d.
4.6mm i.d.
Genesis C8(EC)
3m
1.0mm i.d.
2.1mm i.d.
3.0mm i.d.
4.0mm i.d.
4.6mm i.d.
4m
1.0mm i.d.
2.1mm i.d.
3.0mm i.d.
4.0mm i.d.
4.6mm i.d.
Genesis Phenyl
4m
1.0mm i.d.
2.1mm i.d.
4.0mm i.d.
4.6mm i.d.
Genesis Guards1
30mm
50mm
100mm
150mm
200mm
250mm
10mm
20mm
FK3951E
FL3951E
FH3951E
FM3951E
FJ5951E
FK5951E
FL5951E
FH5951E
FM5951E
FJ10951E
FK10951E
FL10951E
FH10951E
FM10951E
FJ15951E
FK15951E
FL15951E
FH15951E
FM15951E
FK20951E
FM20951E
FJ25951E
FK25951E
FL25951E
FH25951E
FM25951E
FJ1951-2
FK1951-2
FL1951-2
FH1951-2
FK2951-2
FL2951-2
FH2951-2
FK3963E
FL3963E
FH3963E
FM3963E
FK3960E
FL3960E
FH3960E
FM3960E
FJ5963E
FK5963E
FL5963E
FH5963E
FM5963E
FJ5960E
FK5960E
FL5960E
FH5960E
FM5960E
FJ10963E
FK10963E
FL10963E
FH10963E
FM10963E
FJ10960E
FK10960E
FL10960E
FH10960E
FM10960E
FJ15963E
FK15963E
FL15963E
FH15963E
FM15963E
FJ15960E
FK15960E
FL15960E
FH15960E
FM15960E
FK20963E
FM20963E
FK20960E
FM20960E
FJ25963E
FK25963E
FL25963E
FH25963E
FM25963E
FJ25960E
FK25960E
FL25960E
FH25960E
FM25960E
FJ1963-2
FK1963-2
FH1963-2
FJ1960-2
FK1960-2
FL1960-2
FH1960-2
FK2963-2
FH2963-2
FK2960-2
FL2960-2
FH2960-2
FK3968E
FL3968E
FH3968E
FK3962E
FL3962E
FH3962E
FM3962E
FJ5968E
FK5968E
FL5968E
FH5968E
FJ5962E
FK5962E
FL5962E
FH5962E
FM5962E
FJ10968E
FK10968E
FL10968E
FH10968E
FJ10962E
FK10962E
FL10962E
FH10962E
FM10962E
FJ15968E
FK15968E
FL15968E
FH15968E
FJ15962E
FK15962E
FL15962E
FH15962E
FM15962E
FL20968E
FK20962E
FM20962E
FJ25968E
FK25968E
FL25968E
FH25968E
FJ25962E
FK25962E
FL25962E
FH25962E
FM25962E
FJ1968-2
FK1968-2
FL1968-2
FH1968-2
FJ1962-2
FK1962-2
FL1962-2
FH1962-2
FK2968-2
FL2968-2
FH2968-2
FK2962-2
FL2962-2
FH2962-2
FK3969E
FL3969E
FH3969E
FM3969E
FK3964E
FL3964E
FH3964E
FM3964E
FJ5969E
FK5969E
FL5969E
FH5969E
FM5969E
FJ5964E
FK5964E
FL5964E
FH5964E
FM5964E
FJ10969E
FK10969E
FL10969E
FH10969E
FM10969E
FJ10964E
FK10964E
FL10964E
FH10964E
FM10964E
FJ15969E
FK15969E
FL15969E
FH15969E
FM15969E
FJ15964E
FK15964E
FL15964E
FH15964E
FM15964E
FK20969E
FM20969E
FK20964E
FM20964E
FJ25969E
FK25969E
FL25969E
FH25969E
FM25969E
FJ25964E
FK25964E
FL25964E
FH25964E
FM25964E
FJ1969-2
FK1969-2
FL1969-2
FH1969-2
FJ1964-2
FK1964-2
FL1964-2
FH1964-2
FK2969-2
FL2969-2
FH2969-2
FK2964-2
FL2964-2
FH2964-2
FK3980E
FH3980E
FM3980E
FJ5980E
FK5980E
FH5980E
FM5980E
FJ10980E
FK10980E
FH10980E
FM10980E
FJ15980E
FK15980E
FH15980E
FM15980E
FK20980E
FM20980E
FJ25980E
FK25980E
FH25980E
FM25980E
FJ1980-2
FK1980-2
FH1980-2
FH1980-2
FK2980-2
FH2980-2
FH2980-2
Excellent peak symmetry

Exceptional stability from pH 1 to 10
Reduced need for mobile-phase modifiers
Long column life
NOTE: Genesis line is completed by additional phases such as Silica, Phenyl, Cyano, and Amino, for details please check online at www.discoverysciences.com.
1
All Genesis guards listed are cartridges and require either a stand-alone holder or direct-connect holder for use. Guard cartridges are 2/pk.
Guard Cartridge Holders for Genesis and Apex Guards

1.0 mm i.d.
2.1 mm i.d.
3.0, 4.0, 4.6 mm i.d.
10 mm Stand-Alone
20 mm Stand-Alone
10 mm Direct-Connect
20 mm Direct-Connect
F91GPH
F9111P
F9111P
F9112P
F9112P
F9141P
F9151P
F9152P
65
analytical hplc
Jones Apex Reversed-Phase Columns
Economical Column Line with Maximum Reproducibility and Efficiency

Conventional 100 pore size spherical silica
Uniform-sized particle
Narrow particle distribution
Controlled surface area
Apex Phase Specifications
Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
ODS
ODS II
C8
C8(EC)
C1
Diol
Phe
CN
Amino (NH2)
Amino II (NH2)
Carbohydrate
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
3, 5, 10m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
3, 5m
5m
3, 5, 10m
100
100
100
100
100
100
100
100
100
100
100
100
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
170m2/g
10%
10.5%
7%
7%
2.5%
3.5%
3%
4%
2%
2%
n/a%
Polymeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
n/a
Yes
Yes
No
Yes
Yes
No
Yes
No
No
No
Proprietary
No
L1
L1
L7
L7
L13
Apex HPLC Columns

Packing
Format
ODS, 3m
ODS, 5m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
ODS II, 3m
ODS II, 5m
C8, 5m
C8(EC), 5m
Diol, 5m
Phenyl, 5m
Cyano, 5m
Cyano II, 5m
Amino, 5m
Amino II, 3m
Silica, 3m
Silica, 5m
i.d. x Length
Part No.
4.6 x 50mm
4.6 x 30mm
4.6 x 50mm
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 250mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 250mm
4.6 x 30mm
4.6 x 150mm
4.6 x 250mm
4.6 x 30mm
4.6 x 250mm
4.6 x 150mm
4.6 x 100mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
5110394
5109740
5140902
5140900
5140901
5110994
5111025
5109944
5112992
5109730
5109733
5112351
5109921
5109734
5112354
5109735
5120721
5110997
5110650
5109934
5109737
5109933
5112313
5111029
5111004
5109698
5110044
5111024
Apex Guard Columns

Packings
i.d. x Length
Part No.
ODS, 5m
ODS II, 5m
C8, 5m
C8-EC, 5m
Cyano, 5m
Amino, 5m
4 x 10mm
4 x 20mm
4 x 20mm
4 x 10mm
4 x 10mm
4 x 10mm
4 x 20mm
10mm
20mm
5110703
5110705
5112454
5112425
5111079
5111078
5112455
F9111P
F9112P
Guard Cartridge Holder
66
L11
L10
L8
L8
L3
Xanthines in Plasma
1. 7-Methylxanthine
2. 3-Methylxanthine
3. 1-Methylxanthine
4. 1,3-Methyluric Acid
5. Theobromine
6. Ethylxanthine
7. Methyluric Acid
8. Paraxanthine
9. Theophylline
10. 1,3,7-Methyluric Acid
11. Ethyltheophylline
12. Caffeine
11
12
5
2 4
13
0
Column:
Flow Rate:
Mobile Phase:

Gradient:

Column Temp:
Detector:
8
7 10
12
16
20
24
28 Mi
Apex ODS II, C18, 5m, 4.6 x 250mm

0.8mL/min
A: 10 mM NaOAc, 0.02% THF
B: 10 mM NaOAc, 2.7% THF, 25% ACN
Time: 0 35
%B: 0 70
50C
UV at 273nm
more applications

analytical hplc
Alltech OPA Amino Acid

Columns
OPA-HS for high-speed with protein hydrolysates
OPA-HR for high-resolution with physiological samples
OPA-HR (high-resolution) columns are ideal for applications

that require more resolution than simple hydrolysates.
OPA-HR gives baseline separation of most amino acids
found in physiological fluids.
OPA-Derivatized Amino Acids

9234
13
12
Column:
Mobile Phase:

Gradient:

Flow Rate:
Detector:
15
7
4
14
10
56
11
10
15
20
25
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
Aspartic Acid
Glutamic Acid
Serine
Histidine
Glycine
Threonine
Arginine
Alanine
Tyrosine
Methionine
Valine
Phenylalanine
Isoleucine
Leucine
Lysine
30 Min.
OPA-HR, 5m, 4.6 x 150mm (Part No. 28072)

A: Methanol B: Tetrahydrofuran
C: 20mM Sodium Acetate buffer, pH 5.5
Time: 0 20
%A: 5 65
%B: 5 2
%C: 90 23
1.5mL/min
Fluorescence
OPA-HS, 5m
OPA-HR, 5m
Alltech Catecholamine
Columns
For routine analysis of indoles and catecholamines
Reliable and inexpensive
With this column, easily resolve catecholamines under isocratic
conditions at room temperature. A simple monochloroacetic
acid mobile phase with varying amounts of sodium octyl
sulfate and disodium EDTA controls the elution profile.
Catecholamine HPLC Columns
Packing
Catecholamine, 3m
Format
i.d. x Length
Part No.
Analytical
Analytical
4.6 x 100mm
4.6 x 150mm
28064
28072
Format
i.d. x Length
Part No.
Analytical
4.6 x 100mm
28916
All-Guard Cartridges*
Packing
i.d. x Length
Catecholamine, 3m
4.6 x 7.5mm
Qty.
Part No.
3
ea
96076
80101
Alltech NucleotideNucleoside Columns

Determine both nucleotides and nucleosides with a
single column
With this column, separate seven common nucleosides or
twelve common nucleotides using a buffer/methanol/tetrabutyl
ammonium phosphate gradient.
Nucleotide-Nucleoside HPLC Columns
Packing
OPA Amino Acid HPLC Columns

Packing
7110
OPA-HS (high-speed) columns are ideal for the study of

protein acid hydrolysates with very simple mobile-phase
systems.
Nucleotide-Nucleoside, 7m
Format
i.d. x Length
Part No.
Analytical
4.6 x 250mm
35623
All-Guard Cartridges*
Packing
Derivatization Reagents
Description
Qty.
Part No.
OPA Crystals
5g
35606
i.d. x Length
Nucleotide-Nucleoside, 7m
4.6 x 7.5mm
Qty.
Part No.
3
ea
96077
80101
more info
For related products, see our Vydac line of Life Science columns
on pages 85 95.
67
analytical hplc
Alltech Mixed-Mode
Columns
Alltech Carbohydrate
Cation Columns
Separate hydrophobic and ionic species on a

single column
Control ionization with buffer adjustments instead of
ion-pair reagents
High efficiency separations

using only water as the
mobile phase
Sulfonated polystyrene
resin in the calcium form
Column heating required
7110
Anesthetics and Neutral

8780
100% Pure Red Delicious Apple Juice

(a 1:250 Dilution)
8781
2
2
1
1
1. Biphenyl
2. Lidocaine
3. Procaine
8965
1.
2.
3.
4.
5.
Unknown
Sucrose
Glucose
Fructose
Sorbitol
3
2
1
0
Column:
Mobile Phase:

Flow Rate:
Column Temp:
Detector:
10
15
20 Min.
Mixed-Mode C8/Cation, 5m, 4.6 x 150mm

8780: 55mM K2HPO4, pH6.5: Acetonitrile (50:50)
8781: 45mM K2HPO4, pH6.5: Acetonitrile (50:50)
1.0mL/min
30C
UV at 220nm
Changing the buffer concentration affects the capacity of the cationic

components lidocaine and procaine, but does not affect the capacity
of biphenyl, a neutral component.
5
5
10
Column:
lltech Carbohydrate Cation, 10m, 6.5 x 300mm

A
(Part No. 70057)
Mobile Phase: Water
Flow Rate:
0.5mL/min
Column Temp: 90C
Detector:
Alltech ELSD
Carbohydrate Cation HPLC Columns

Packing
Format
Carbohydrate Cation, 10m Analytical

Mixed-Mode HPLC Columns
Packing
Format
Analytical
Analytical
C8/Cation, 5m Analytical
Analytical
C18/Cation, 5m Analytical
Analytical
C8/Anion, 7m
i.d. x Length
Part No.
Waters
Fittings
Part No.
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
71354
12369
72568
71364
72574
71369
71355
12370
72569
71365
72575
71370
Mixed-Mode All-Guard Cartridges*

Packing, 5m
i.d. x Length
C8/Anion
4.6 x 7.5mm
C8/Cation
4.6 x 7.5mm
C18/Cation
4.6 x 7.5mm
Qty.
Part No.
3
3
3
ea
96147
71379
71380
80101
15 Min.
i.d. x Length
Part No.
6.5 x 300mm
70057
Carbohydrate Cation All-Guard Cartridges

Packing
i.d. x Length
Carbohydrate Cation Guard Cartridges

4.6 x 7.5mm
Qty.
Part No.
96109
80101
more info
For Alltech Prevail Carbohydrate ES columns, see page 47.
related product
Need a column heater?

See pages 16 and 17.
related product
6219
Doing carbohydrate analysis?

Alltech 3300 ELSD is more
sensitive than RI. See pages 5 10.
6947B
68
analytical hplc
Vydac Venture Immunoaffinity Columns

Repetitive-Use, Silica-Based Immunoaffinity Columns
6908
Venture immunoaffinity HPLC columns provide an easy and quick way to purify and concentrate analytes from complex food,
environmental, and biological matrices for fast, accurate measurement.
Benefits
Rapid Analysis
Features
No laborious sample preparation
procedures
Fast binding kinetics
Excellent Accuracy and

Precision
Wide-pore silica support allows online coupling with HPLC for real time
direct analysis
Quantitative recovery of analyte from
IAC column
High sensitivity due to high volume
injection
Precision comparable with
conventional HPLC methods
Specially treated wide-pore silica
eliminates non-specific interactions
Highly Selective
Secondary separation reduces errors

due to cross reactivity
Long Column Lifetime
Analysis of Difficult Matrices
Significant Cost Reductions
Highly selective antibodies toward

target analyte
Analyze over 200 samples on a single
column without column deterioration
due to the high ligand stability of the
Venture IAC columns
Venture IAC columns isolate,
concentrate and purify targeted
sample components from dilute and
complex matrices in one step
Costs reduced by as much as 90%
Long column lifetime decreases
material costs to less than $5.00
per analysis
Venture Immunoaffinity Columns

Product Name
Target Analyte
Mycotoxins Analysis
Venture AF
Aflatoxin
Venture OT
Ochratoxin A
Food Nutrient Analysis
Venture FA
Folic Acid
Venture B12 Vitamin B12
Venture BF
Folic Acid and Vitamin B12
Acrylamide Analysis
Venture AC
Acrylamide
Lactoferrin Analysis
Venture LTF Lactoferrin
Endocrine Disruptors Analysis
Venture EE2 17-Ethynyl Estradiol
Venture E2
17-Estradiol
Venture E1
Estrone
Venture BPA Bisphenol A
Pollutants Analysis
Venture CPA Chlorophenoxy Acetic Acid
Herbicides
Venture PVH Phenylurea Herbicides
Venture OPP Organophosphorous
Pesticides
Venture VCZ Vinclozolin Fungicide
Steroid Hormones Analysis
Venture TT
Testosterone
Venture NT
Nortestosterone
i.d. and length
Part No.
2.1 x 50mm
2.1 x 50mm
5120313
5135954
2.1 x 50mm
2.1 x 50mm
2.1 x 50mm
5135952
5120201
5135951
2.1 x 50mm
5135953
2.1 x 50mm
5120400
2.1 x 50mm
2.1 x 50mm
2.1 x 50mm
2.1 x 50mm
5120404
5120403
5120405
5120406
2.1 x 50mm
5120407
2.1 x 50mm
2.1 x 50mm
5120408
5120410
2.1 x 50mm
5120411
2.1 x 50mm
2.1 x 50mm
5120401
5120402
Vydac Venture immunoaffinity columns use ICEtech (Inert Coating Enhancement Technology, U.S. Patent No. 6,802,966)
silica processing to eliminate non-specific binding on the silica surface. This enables Venture columns to be the first affinity
matrix to take advantage of silicas rigid porous structure, making direct coupling of the columns with other modes
of chromatography, such as reversed-phase HPLC feasible.
Rapid analysis and automation

reduces technician time
Significant reduction in solvent use
Automated Analysis
Less solvent waste disposal costs

HPLC format allows automation for
high sample throughput
Vydac Denali C18 Reversed Phase

Denali columns contain a new-generation silica-based 120 monomeric C18 adsorbent
based on the same bonding technology employed in Vydac Everest 300 columns. This new
technology improves C18 surface coverage and deactivates residual silanols. Previously, the
best monomeric C18 chemistries had carbon coverage in the 2.8 to 3.6moL/m2 range. Denali
C18 has coverage in excess of 4moL/m2 and approximates the theoretical limit based on
surface area.
7110
Vydac Denali C18 Reversed-Phase 238DE C18

Particle Size
3m
5m
i.d.
50mm
2.1mm3
4.6mm3
1.0mm3
2.1mm3
4.6mm3
238DE3205
238DE3405
238DE5105
238DE5205
238DE5405
Columns
100mm
238DE3210
238DE3410
238DE5110
238DE5210
238DE5410
150mm
250mm
238DE5115
238DE5215
238DE5415
238DE51
238DE52
238DE54
Recommended Guards
Guard Kit1
Guard Cartridge2
238GK34DE
238GK51DE
238GK52DE
238GK54DE
238GD34DE
238GD51DE
238GD52DE
238GD54DE
NOTE: Additional column diameters and lengths are available on request. Please contact Grace Davison Discovery Sciences to discuss your requirements.
1
A guard kit includes a holder and one guard cartridge. 2Guard cartirdge units include two guard cartridges. 3Titanium frits are standard in column diameters 4.6mm and smaller.
69
analytical hplc
Vydac 201TP and 202TP C18
Specialty Reversed Phases for PAH and Vitamins

201TP: The standard for PAH analysis in all types of environmental samples. Vydac 201TP
columns separate the EPA 16 priority pollutants in less than 20 minutes.
202TP3405: Rapid analysis to separate the 16 priority pollutant PAHs in under 10 minutes
202TP54 and 202TP5415: For the analysis of derivatized PAHs
Vydac 201TP and 202TP C18 Specifications

Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
201TP C18
202TP C18
Silica
Silica
Spheroidal
Spheroidal
5, 7, 10, 1015, 1520m

3, 5, 10m
300
300
7090m2/g
6090m2/g
8%
9%
Polymeric
Polymeric
No
No
L1
L1
Vydac 201TP
Vydac 201TP HPLC columns have long been the standard for the analysis of PAHs in water, air, soil, automotive exhaust,
and food. They were used to establish standard reference materials, measure air quality, measure PAHs in sediments,
quantify PAHs in food, and study high molecular weight PAHs. They have also been used in the study of shape selectivity
of reversed-phase materials.
Priority Pollutants PAHs

In Accordance with
EPA Methods 505, 550.1, 610, and 8310
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
Naphthalene
Acenaphthylene
Acenaphthene
Fluorene
Phenanthrene
Anthracene
Fluoranthene
Pyrene
PAHs Beyond the EPA Priority Pollutants

1. Naphthalene
2. Acenaphthylene
3. 1-Methylnaphthalene
4. 2-Methylnaphthalene
5. Acenaphthene
6. Fluorene
7. Phenanthrene
8. Anthracene
9. Fluoranthene
10. Pyrene
11. Benzo[c]phenanthrene
12. Cyclopenta[cd]pyrene
Benz[a]anthracene
Chrysene
Benzo[b]fluoranthene
Benzo[k]fluoranthene
Benzo[a]pyrene
Dibenz[ah]anthracene
Benzo[ghi]perylene
Indeno[1,2,3-cd]pyrene
5 6
11
13 16
15
14
11
13
Column:
15 Mi
ydac C18, 5m, 4.6 x 150mm

V
(Part No. 201TP5415)
Flow Rate:
1.5mL/min
Mobile Phase: A: Water B: ACN
Gradient:
Hold 50% B for 3min, then 50 to 100% B in 7min
Detector:
UV at 254nm
more applications

Column:
Benz[a]anthracene
Chrysene
Benzo[b]naptho[2,1-d]thiopen
7,12-Dimethylbenz[a]anthracene
Benzo[e]pyrene
Benzo[a]pyrene
Benzo[ghi]perylene
18
1
12
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
10
70
7110
Vydac 201TP and 202TP columns were developed specifically for the separation and quantification of PAHs required by
environmental regulations, current and future. Beyond the 16 EPA priority pollutant PAHs, Vydac PAH columns are used to
separate many other PAHs, such as methylated naphthalenes.
17
14
16 19
23
13
20
1112 15
22
10
21
5
4
3
12
16
20
24
28
32 Mi
ydac C18, 5m, 4.6 x 150mm

V
Flow Rate:
1.0mL/min
Gradient:
50 to 100% B over 30min
Column Temp: 30C
Detector:
UV at 254nm
analytical hplc
Vydac 201TP (continued)
Vydac 202TP
Vitamins
Built on top of the success of 201TP, 202TP columns are

ideal for derivatized PAH samples or high throughput PAH
analyses.
Vydac 201TP columns also have application in the analysis

of carotenoids, retinoids, and vitamins.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
All-trans-retinol (vitamin A)
Tocol
g-Tocopherol
a-Tocopherol (vitamin E)
Lutein
Zeaxanthin
Cryptoxanthin
a-Carotene
All-trans-b-carotene
Cis-b-carotene
325 nm
12
16
20
9
8 10
24
28
Benz[a]anthracene
Chrysene
Benzo[a]pyrene
Benzo[ghi]perylene
11
9.
10.
11.
12.
13.
14.
15.
16.
Naphthalene
Acenaphthylene
Acenaphthene
Fluorene
Phenanthrene
Anthracene
Fluoranthene
Pyrene
450 nm
7
4
1.
2.
3.
4.
5.
6.
7.
8.
295 nm
Rapid Analysis of Polyaromatic Hydrocarbons
10
9
12
13 14 16
15
32 Mi
Column:
ydac 201TP C18, 5m, 4.6 x 250mm

V
(Part No. 201TP54)
Flow Rate:
1.5mL/min
Mobile Phase: A: 15:75:10 Buffer/Methanol/n-Butanol
B: 2:88:10 Buffer/Methanol/n-Butanol
Buffer = Aqueous 0.02 M NH4OAc, pH 3.5
Gradient:
100% A for 3min, then 0 to 100% B over 15min,
then hold 100% B
Detector:
UV, programmed wavelengths
Retinol, a-Tocopherol, and b-Carotene in Serum
8 Mi
Column:
ydac 202TP C18, 5m, 4.6 x 50mm

V
Flow Rate:
3.0mL/min
Gradient:
40 to 95% B in 8min
Column Temp: 30C
Detector:
UV at 254nm
Chromatogram reproduced with authors permission. From W.A. MacCrehan and

E. Schonberger, Clin. Chem., 33(9), 1585-1592 (1987).
201TP C18 Analytical Columns

Particle Size
5m
10m
1
i.d.
50mm
1.0mm
2.1mm
3.2mm
4.6mm
4.6mm
201TP5105
201TP5205
201TP5405
Columns
100mm
201TP5210
201TP5410
150mm
250mm
201TP5115
201TP5215
201TP5315
201TP5415
201TP10415
201TP51
201TP52
201TP53
201TP54
201TP104
Recommended Guards
Guard Kit1
Guard Cartridge2
201GK51T
201GK52T
201GK54T
201GK54T
201GK104T
201GD51T
201GD52T
201GD54T
201GD54T
201GD104T
A guard kit includes a holder and one guard cartridge. 2Guard cartridge units include two guard cartridges.
202TP High-Carbon-Load C18 Analytical Columns

Particle Size
3m
5m
10m
1
i.d.
50mm
3.2mm
4.6mm
4.6mm
4.6mm
202TP3405
202TP10405
Columns
100mm
202TP3410
150mm
250mm
202TP3315
202TP5415
202TP54
Recommended Guards
Guard Kit1
Guard Cartridge2
202GK54T
202GK104T
202GD54T
202GD104T
A guard kit includes a holder and one guard cartridge. 2Guard cartridge units include two guard cartridges.
71
analytical hplc
Astec Amino and

Reversed-Phase Columns
Durable vinyl alcohol copolymer
Stable from pH 213
Stable and reproducible performance
Choose Astec reversed-phase columns for amines and high

recoveries of proteins and peptides. Because there are no
silanols, elution order is always based on hydrophobicity
of the analytes rather than polar interactions with the base
material. High recovery of proteins and peptides is typical
even at low sample loads.
Mono-, Di-, and Trisaccharides

9103
1. Rhamnose
2. Fructose
3. Glucose
4. Sucrose
5. Maltose
6. Raffinose
3 4
5
10
Column:
Mobile Phase:
Flow Rate:
Detector:
Astec Amino, 5m, 4.6 x 250mm (Part No. 16012)

Acetonitrile:Water (75:25)
1.0mL/min
RI
Cyclobond I 2000 has the broadest applicability, and

is ideal for small analytes in pharmaceutical, chemical,
and environmental applications.
Cyclobond 1 2000 Ac is ideal for aromatic alcohols
or amines that are chiral on the alpha or beta carbon.
Cyclobond I 2000 RSP is a general-purpose chiral
stationary phase. It can separate non-aromatic structures
such as t-boc amino acids.
Cyclobond DMP shows good selectivity when the
chiral centers are part of a ring structure or on the alpha
carbon. This phase is ideal for derivatized amines such
as amphetamine ACQ.
Format
Analytical
Analytical
I 2000 Ac, 5m
Analytical
I 2000 RSP, 5m Analytical
I 2000 DMP, 5m Analytical
II
Analytical
Analytical
i.d. x Length
Part No.
C18, 5m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
16004
16005
16006
16007
16008
16009
16012
Packing
i.d. x Length
Qty.
Part No.
C18, 5m
C8, 5m
C4, 5m
Amino, 5m
4.6 x 10mm
4.6 x 10mm
4.6 x 10mm
4.6 x 10mm
ea
ea
ea
ea
28180
28181
28182
28187
i.d. x Length
Part No.
4.6 x 100mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 100mm
4.6 x 250mm
400101
410101
410121
411121
412111
400201
410201
Astec Cyclobond Guard Cartridges*
Astec Amino and Reversed-Phase Guard Columns
72
-Cyclodextrin
-Cyclodextrin Acetylate
-Cyclodextrin R,S-Hydroxypropyl Ether
-Cyclodextrin 3,5-Dimethylphenyl Carbamate
-Cyclodextrin
I 2000, 5m
Format
Amino, 5m
Substituent
I 2000
I 2000 Ac
I 2000 RSP
I 2000 DMP
II
Packing
Packing
C4, 5m
Designation
Astec Cyclobond Columns
30 Min.
Astec Amino and Reversed-Phase 300 Columns
C8, 5m
Versatile chiral selectors

High-purity 5m spherical silica
Cyclobond II is useful for isomeric compounds based

on anthracene, chrysene, and pyrene ring structures.
Some applications include steroids, porphyrins, and FMOC
amino acids.
20
For Chiral Separations
Cyclodextrin Phases
Choose Astec amino columns for separating mono- and

oligosaccharides. Astecs polymer base makes these columns
much more stable than silica-based amino columns.
Astec Cyclobond Columns
Packing,
i.d. x Length
I 2000, 5m
4.0 x 20mm
I 2000 Ac, 5m
4.0 x 20mm
I 2000 RSP, 5m
4.0 x 20mm
I 2000 DMP, 5m
4.0 x 20mm
II
4.0 x 20mm
Astec Guard Cartridge Holder
Qty.
Part No.
ea
ea
ea
ea
ea
ea
430102
430103
430105
430108
430109
11014
analytical hplc
Astec Chirobiotic
HPLC Columns
Naproxen
8769
For Multi-Mode Chiral Separations
CH3
Chirobiotic columns demonstrate a broad selectivity in

reversed phase, normal phase, and polar organic modes.
This gives Chirobiotic columns the ability to separate a
greater variety of chiral analytes than columns that can only
operate in one mode.
Chirobiotic V
CHCOH
O
6
10
Column:
Mobile Phase:
Flow Rate:
Detector:
12 Min.
H3CO
Chirobiotic V, 4.6 x 250mm (Part No. 11024)

THF:0.1% Triethylammonium Acetate, pH 7 (10:90)
1.0mL/min
UV at 254nm
Chirobiotic V Specifications
Ligand:
Vancomycin
Ideal For:
Neutral molecules, amides, acids, esters, cyclic amines
Phenylalanine
8770
Chirobiotic V has a selectivity similar to glycoprotein phases

while also being stable from 0 100% organic modifiers. New
Chirobiotic V2 has enhanced selectivity and capacity in the
polar organic mode, and increased capacity.
Chirobiotic T
Chirobiotic T Specifications
Ligand:
Teicoplanin
Ideal For:
Underivatized amino acids, n-derivatized amino acids,
carboxylic acids, phenols, neutral aromatics, cyclic aromatics
with aliphatic amines
Chirobiotic T is an excellent alternative to crown ether and

ligand exchange for amino acids and hydroxy acids. New
Chirobiotic T2 has enhanced selectivity and capacity in the
polar organic mode.
OH
NH2
4 6 8 10 12 Min.
Column:
Mobile Phase:
Flow Rate:
Detector:
Chirobiotic T, 4.6 x 250mm (Part No. 12053)

Ethanol:Water (80:20)
1.0mL/min
UV at 210nm
Astec Chirobiotic Columns

Packing
Format
i.d. x Length
Part No.
Chirobiotic V
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Prep
Prep
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Prep
Prep
Analytical
Analytical
Analytical
4.6 x 50mm
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 250mm
10 x 250mm
21.2 x 250mm
4.6 x 50mm
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 250mm
10 x 250mm
21.2 x 250mm
4.6 x 50mm
4.6 x 150mm
4.6 x 250mm
11346
11023
11024
A15022
A15023
A15024
A15034
A15044
11349
12051
12053
A16022
A16023
A16024
A16034
A16044
12514
12516
12518
Chirobiotic R
Chirobiotic R Specifications
Ligand:
Ristocetin A
Ideal For:
Anionic molecules, di- and tri-peptides, -hydroxy acids,
substituted aliphatic acids, aromatic esters, chiral alcohols,
secondary and tertiary amines
Chirobiotic R is the largest and most complex of the

Chirobiotic ligands. Sugar moieties, a peptide chain, and
additional ionizable groups give this structure the complexity
and diversity to separate a wide variety of analytes.
Bonded macrocyclic glycopeptide phases

Three complimentary selectivities
High-purity 5m spherical silica
Chirobiotic V2
Chirobiotic T
Chirobiotic T2
Chirobiotic R
Astec Chirobiotic Guard Cartridges*

Packing
i.d. x Length
Chirobiotic V, 5m
4.0 x 20mm
Chirobiotic T, 5m
4.0 x 20mm
Chirobiotic R, 5m
4.0 x 20mm
Astec Guard Cartridge Holder
Qty.
Part No.
ea
ea
ea
ea
11019
12445
12522
11014
73
analytical hplc
Brownlee Columns
hplc columns | small and large molecule
Economical MPLC Cartridge System with

Reusable Hardware
Finger-tight modular design
Reusable end assemblies with interchangeable holders
Guards fit conventional columns or MPLC hardware
The Brownlee MPLC Cartridge system is convenient
and easy to use. Decide which column length you prefer,
and purchase the hardware and the disposable packed
cartridges separately.
Cartridge Hardware
Rugged and reproducible

Use with MPLC cartridge hardware
80, spherical silica
RP-18 and RP-8 phases are monomeric for optimum mass
transfer kinetics and high efficiency. The ODS phase is
polymeric and more suitable for acidic mobile phases.
Brownlee Spheri-5 Cartridges*
Packing
i.d. x Length
Qty.
Part No.
ODS (C18), 5m
2.1 x 30mm
2.1 x 100mm
2.1 x 220mm
4.6 x 30mm
4.6 x 100mm
4.6 x 220mm
2.1 x 30mm
2.1 x 100mm
2.1 x 220mm
4.6 x 100mm
4.6 x 220mm
2.1 x 30mm
2.1 x 100mm
2.1 x 220mm
4.6 x 30mm
4.6 x 100mm
4.6 x 220mm
4.6 x 30mm
4.6 x 100mm
4.6 x 220mm
4.6 x 30mm
4.6 x 100mm
4.6 x 220mm
2.1 x 30mm
4.6 x 30mm
4.6 x 100mm
4.6 x 220mm
2
ea
ea
2
ea
ea
2
ea
ea
ea
ea
2
ea
ea
2
ea
ea
2
ea
ea
2
ea
ea
2
2
ea
ea
141061
141063
141065
141060
141062
141064
141052
141054
141056
141053
1410551
141028
141030
141032
141027
141029
141031
141083
141085
141087
141177
141179
141181
141194
141193
141195
141197
Brownlee MPLC Cartridge Hardware

Photo
Description
Part No.
30mm
140200
100mm
140230
220mm
140400
MPLC Cartridge Union
140300
MPLC Cartridge End

Assembly
1402501
30mm
140203
100mm
140003
220mm
140403
MPLC Cartridge Holders

5424
0647A
MPLC Cartridge Accessories
RP-18, 5m
RP-8, 5m
0647B
0647C
MPLC Cartridge Holder Bodies
0647D
Universal MPLC Cartridge Holder Kit

Includes: 30-, 100-, and 220mm MPLC Cartridge Holder
Bodies (E), 1 MPLC Cartridge Union (C), 2 End Assemblies
(D), and 1 NewGuard End Assembly (H)
140530
Brownlee MPLC Cartridge Hardware Seals

Part No.
Analytical Holder Seal Replacement Kit

Includes Tool and 2 Seals
Analytical Seals, 2/pk
Seal Replacement Tool Required
140260
140216
0647E
0647F
Amino, 5m
Silica, 5m
Brownlee Spheri-10 Cartridges*

Packing
i.d. x Length
Qty.
Part No.
RP-18, 10m
4.6 x 30mm
4.6 x 100mm
4.6 x 30mm
4.6 x 100mm
4.6 x 30mm
2
ea
2
ea
2
141043
1410451
141020
141022
141186
RP-8, 10m
Brownlee MPLC NewGuard Hardware

Photo
Cyano, 5m
*Brownlee MPLC cartridge holder required.
Description
NewGuard System
Description
Part No.
NewGuard Cartridge
Holders NewGuard Holder
for use with Conventional
Columns
220mm NewGuard
System Holder for Direct
Coupling of 220mm
Analytical Cartridge with a
NewGuard Cartridge
NewGuard End Assembly
140601
RP-18
RP-8
Cyano
Amino
Silica
Anion (AX-300)
*NewGuard cartridge holder required (Part No. 140601).
Large pore for macromolecules

Choose AX-300 with its DEAE functional group for weak anion
exchange applications with proteins and peptides.
140600
Brownlee NewGuard Cartridges*

Description
Silica, 10m
Aquapore DEAE WAX Cartridges

140410
0647G
74
Spheri-5 and Spheri-10 Cartridges
Qty.
Part No.
3
3
3
3
3
3
141004
141003
141008
141007
141012
141009
Brownlee Aquapore Cartridges*

Packing
i.d. x Length
Qty.
Part No.
AX-300, 7m
4.6 x 30mm
4.6 x 100mm
2
ea
141212
141214
analytical hplc
Jordi Columns
GPC Columns
Normal- and Reversed-Phase
Jordi normal-phase and reversed-phase columns are

made from highly pure 5m DVB, for extremely durable
and efficient phases.
Jordi Specifications
Phase
Particle Size
Pore Size
Description
RP-DVB
5m
500, 1000
C18-DVB
5m
500
Polyamine-DVB
5m
500
Reversed-phase,
not bonded
Reversed-phase,
C18 bonded
Normal-phase
RP-DVB
RP-DVB is a reversed-phase non-polar packing with a high
degree of aromatic character. SM-500, with a 500 pore, is
recommended for most applications. LM-1000, with a 1000
pore, is ideal for molecules 100,000 Daltons or larger.
Organic GPC
Rugged highly crosslinked DVB for broad temperature
stability and solvent compatibility
Powerful high pore volume for greater resolution from
a single column
Jordi Organic and Aqueous GPC Specifications
Pore Size
Pressure Limit
MW Range (Daltons)
100
500
103
104
105
Mixed Bed
8000psig
8000psig
8000psig
2000psig
2000psig
2000psig
1005000
10010,000
10025,000
1002,000,000
10,00010,000,000
100>10,000,000
Jordi HPLC columns, offer unparalleled resistance to

shrinking and swelling, as well as better temperature and
solvent compatibility than traditional ps-DVB phases.
The high pore volume speeds up your analyses. A single
10 x 500mm Jordi column replaces three 7.8 x 300mm ps-DVB
columns and separates the same sample in 40% less time.
C18-DVB
Aqueous GPC
C18-DVB has C18 chains bonded to the DVB base. These

columns are more hydrophobic than traditional C18 columns
and have different selectivities due to the absence of silanols.
Glucose-DVB packings are rugged, hydrophilic packings for

separating polar compounds. Glucose units are bonded to
the DVB base to yield a hydrophilic surface. Glucose-DVB
columns equilibrate faster than silica-based columns.
Polyamine-DVB
Polyamine-DVB is a normal-phase column for simple sugar
and polysaccharide applications. The DVB-based packing
provides a non-reactive amine surface that lasts longer and
equilibrates faster than traditional silica-based amine columns.
Polyamine-DVB columns can be used with aqueous mobile
phases, even 1M NaOH. Ideal for carbohydrate analyses
requiring sharp peaks and short run times.
Jordi HPLC Columns
Packing
Format
Analytical
Analytical
RP-DVB (LM-1000), 5m
Analytical
Analytical
C18-DVB (500), 5m
Analytical
Analytical
Polyamine-DVB (500), 5m Analytical
RP-DVB (SM-500), 5m
i.d. x Length
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
Part No.
1005500
1005520
1005580
1005585
18500
18501
1005900
Jordi All-Guard Cartridges*

Packing
i.d. x Length
RP-DVB (SM-500), 5m
4.6 x 7.5mm
RP-DVB (LM-1000), 5m
4.6 x 7.5mm
C18-DVB (500), 5m
4.6 x 7.5mm
Polyamine-DVB (500), 5m 4.6 x 7.5mm
Qty.
Part No.
3
3
3
3
ea
1005595
1005596
1005601
1005615
80101
related product
Looking for column heaters?

Ruggeduse at high temperatures, from pH 014,

with any solvent
Silanol Freeanalyze polar compounds without
silanol effects
Jordi Organic GPC Columns*

Pore Size
Format
Semi Prep
Prep
500, 5m
Semi Prep
Prep
Prep
103, 5m
Semi Prep
Prep
104, 5m
Semi Prep
Prep
105, 5m
Semi Prep
Prep
Mixed-Bed Linear, 5m Semi Prep
Prep
Prep
100, 5m
i.d. x Length
Part. No.
7.8 x 300mm
10 x 250mm
7.8 x 300mm
10 x 250mm
10 x 500mm
7.8 x 300mm
10 x 250mm
7.8 x 300mm
10 x 250mm
7.8 x 300mm
10 x 250mm
7.8 x 300mm
10 x 250mm
10 x 500mm
10510
100561
10511
100565
100567
10512
100569
10513
100573
10514
100577
10515
100581
100583
*Jordi GPC columns are constructed from Type 316 stainless steel. All tubing
connections are 1/16" female. Columns are shipped in Chloroform unless otherwise
requested when placing order.
Jordi Aqueous GPC Columns

Pore Size
Format
Semi Prep
Prep
500, 5m
Semi Prep
Prep
103, 5m
Semi Prep
Prep
104, 5m
Semi Prep
Prep
105, 5m
Semi Prep
Prep
Mixed-Bed Linear, 5m Semi Prep
Prep
Prep
100, 5m
i.d. x Length
Part No.
7.8 x 300mm
10 x 250mm
7.8 x 300mm
10 x 250mm
7.8 x 300mm
10 x 250mm
7.8 x 300mm
10 x 250mm
7.8 x 300mm
10 x 250mm
7.8 x 300mm
10 x 250mm
10 x 500mm
10517
100761
10518
100765
10519
100769
10520
100773
10521
100777
10522
100781
100783
6219
75
analytical hplc
Merck LiChrosorb and LiChrospher Columns
LiChrosorbirregular silica for larger surface areas

LiChrospherspherical silica for higher stability
and reproducibility
Melatonin
9055
All Merck columns are packed by Grace to our highest

QC standards. Custom columns are available.
1. Melatonin
LiChrosorb HPLC Columns

Packing
Format
RP-18, 5m
Solvent Reducer
Solvent Reducer
Analytical
Analytical
Analytical
Solvent Reducer
Solvent Reducer
Analytical
Analytical
Analytical
Analytical
RP Select B, 5m
RP-8, 5m
Amino, 5m
Si-60, 10m
Si-100, 10m
i.d. x Length
Part No.
3.0 x 150mm
3.0 x 250mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
3.0 x 150mm
3.0 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
38513
38514
8684
228700
228685
38515
38516
8692
228872
8369
8720
Column:

Mobile Phase:
Flow Rate:
Detector:
8 Min.
LiChrospher RP-18, 5m, 4.6 x 150mm

(Part No. 228695)
25mM KH2PO4, pH2.75:MeOH (60:40)
1.0mL/min
UV at 254nm
LiChrospher HPLC Columns

Packing
Format
RP-18, 3m
RP-18, 5m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
RP-18 EC, 3m
RP-18 EC, 5m
RP-8, 5m
RP-8 EC , 5m
RP-Select B, 5m
Alltech All-Guard
System change cartridges
in seconds without tools.
Amino, 5m
Diol-100, 5m
Si-60, 5m
i.d. x Length
Part No.
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
228688
228695
228673
228686
228694
228672
228696
228675
228697
228678
228611
228613
228693
228670
228691
228666
228699
228683
1656
LiChrospher All-Guard Cartridges*
LiChrosorb All-Guard Cartridges*

Packing
i.d. x Length
3.0 x 7.5mm
4.6 x 7.5mm
RP-Select B, 5m
4.6 x 7.5mm
RP-8, 5m
3.0 x 7.5mm
4.6 x 7.5mm
Amino, 5m
4.6 x 7.5mm
Silica, 5m
4.6 x 7.5mm
RP-18, 5m
76
Packing
Qty.
Part No.
3
3
3
3
3
3
3
ea
96374
96171
96181
96373
96173
96175
96201
80101
i.d. x Length
RP-18, 5m
4.6 x 7.5mm
RP-18 Endcapped, 5m
4.6 x 7.5mm
RP-8, 5m
4.6 x 7.5mm
RP-8 Endcapped, 5m
4.6 x 7.5mm
RP Select B, 5m
4.6 x 7.5mm
Amino, 5m
4.6 x 7.5mm
Diol, 5m
4.6 x 7.5mm
Silica, 5m
4.6 x 7.5mm
Qty.
Part No.
3
3
3
3
3
3
3
3
ea
96185
96183
96189
96187
96425
96191
96195
96201
80101
analytical hplc
Macherey-Nagel Nucleosil Columns

100, high-purity silica
Narrow particle size distribution
A choice of phases for any application
Nucleosil HPLC Columns
C18 AB is polymerically bonded specifically for reversedphase chromatography of acidic and basic compounds.
It has a 25% carbon load for strong retention.
Format
i.d. x Length
Part No.
C18, 3m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
89531
89511
89161
89141
89121
11652
11657
89221
89201
89181
89261
89241
89321
89301
89281
89381
89361
89341
89441
89421
89401
228498
228497
228496
228501
228500
228499
89532
89512
89162
89142
89122
89222
89202
89182
89262
89242
89322
89302
89282
89382
89362
89342
89442
89422
89402
228504
228503
228502
228507
228506
228505
C18, 5m
C18, 10m
C18 AB, 5m
C8, 5m
SRM 869
9320
C8, 10m
Phenyl, 7m
Cyano, 5m
Column:

Mobile Phase:
Flow Rate:
Detector:
Column Temp:
1. Acetone
2. Phenanthroanthrene
3. 1,2:3,4:5,6:7,
8-Tetrabenzonaphthalene
4. Benzo[a]pyrene
4
5
Cyano, 10m
Amino, 5m
Amino, 10m
Silica, 5m
Silica, 10m
SA, 5m
9 Min.
SA, 10m
SB, 5m
Nucleosil C18 AB, 5m, 4.6 x 150mm

(Part No. 11652)
(85:15) Acetonitrile:Water
2.0mL/min
UV at 254nm
25C
SB, 10m
All Nucleosil columns are packed by Grace to our highest

QC standards.
Packing
Waters
Fittings
Part No.
Alltech All-Guard
System change cartridges
in seconds without tools.
Opium Alkaloids
5243
2
1
5
3
4
1656
1.
2.
3.
4.
5.
6.
12 16 Min.
Column:
Nucleosil CN, 10m, 4.6 x 250mm

(Part No. 89281)
Mobile Phase: 80%15mM Ammonium Acetate pH 5.8
10% Acetonitrile 10% Dioxane
Flow Rate:
1.5mL/min
Detector:
UV at 210nm
Column Temp: Ambient
Morphine
Codeine
Cryptopine
Thebaine
Narcotine
Papaverine
Nucleosil All-Guard Cartridges*

Packing
i.d. x Length
C18, 5m
4.6 x 7.5mm
C8, 5m
4.6 x 7.5mm
Phenyl, 5m
4.6 x 7.5mm
Cyano, 5m
4.6 x 7.5mm
Amino, 5m
4.6 x 7.5mm
Silica, 5m
4.6 x 7.5mm
SA, 5m
4.6 x 7.5mm
SB, 5m
4.6 x 7.5mm
Qty.
Part No.
3
3
3
3
3
3
3
3
ea
96210
96211
96214
96213
96212
96215
89340
89345
80101
more info
For more HPLC Applications, see pages 395 453.
more applications

77
analytical hplc
Whatman Partisil Columns
All Whatman Partisil columns are packed by Grace to our

highest QC standards. Partisil columns use 85, irregular
silica with a surface area of 350m2/g.
Partisil PAC is a polar amino cyano phase with secondary
amino groups for thermal and chemical stability. It separates
primarily by reversed-phase or weak anion exchange.
Whatman Partisphere
WVS Columns
Whatmans Partisphere WVS columns have built-in void
compensators that help you extend the column lifetime.
Use the piston-like plungers at each end of the column to
compress the bed and seal any voids that develop over time.
Choose your column from the table below, and purchase
reusable WVS endfittings separately.
Partisil HPLC Columns

Packing
Format
i.d. x Length
Part No.
Waters
Fittings
Part No.
ODS, 10m
ODS-2, 5m
ODS-2, 10m
ODS-3, 5m
ODS-3, 10m
C8, 5m
C8, 10m
PAC, 5m
PAC, 10m
Silica, 5m
Silica, 10m
SAX, 5m
SAX, 10m
SCX, 10m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
8246
12571
8251
8660
8256
8668
8380
8676
8261
8266
8271
12576
8165
8173
8247
12572
8252
8662
8257
8670
8381
8678
8262
8267
8272
12577
8167
8175
Qty.
Part No.
3
3
3
3
3
3
3
3
ea
96252
96253
96254
96255
96256
96257
96259
96258
80101
Partisil All-Guard Cartridges*

Packing
i.d. x Length
ODS, 5m
4.6 x 7.5mm
ODS-2, 5m
4.6 x 7.5mm
ODS-3, 5m
4.6 x 7.5mm
C8, 5m
4.6 x 7.5mm
PAC, 5m
4.6 x 7.5mm
Silica, 5m
4.6 x 7.5mm
SAX, 5m
4.6 x 7.5mm
SCX, 5m
4.6 x 7.5mm
Partisphere WVS HPLC Cartridges*

Packing
Format
i.d. x Length
C18, 5m
Analytical
C8, 5m
Analytical
PAC, 5m
Analytical
Silica, 5m
Analytical
SAX, 5m
Analytical
SCX, 5m
Analytical
WVS Reusable Endfittings, 2/pk
4.6 x 125mm
4.6 x 125mm
4.6 x 125mm
4.6 x 125mm
4.6 x 125mm
4.6 x 125mm
Part No.
46210502
46210503
46210508
46210501
46210505
46210507
46310001
*Reusable endfittings required.
Partisphere WVS Guard Cartridges*

Packing
i.d. x Length
Qty.
Part No.
C18/C8, 5m
PAC, 5m
Silica, 5m
SAX, 5m
SCX, 5m
Guard Cartridge Holder
5 x 17mm
5 x 17mm
5 x 17mm
5 x 17mm
5 x 17mm
19 x 40mm
5
5
5
5
5
ea
46410002
46410008
46410001
46410005
46410007
46311004
more info
For HPLC Applications, see pages 395 453.
related products
Need HPLC tubing?
See pages 384 391.
5535


related products
Looking for high-pressure fittings?

See pages 112 118.
6673
78
analytical hplc
Polymer Labs Columns

Outstanding Durability and Stability
PLRP-S HPLC Columns
Ideal for elevated temperature and pressure applications

Stable from pH 113
Format
i.d. x Length
100, 3m
Microbore
Microbore
Analytical
Analytical
Microbore
Microbore
Microbore
Analytical
Analytical
Analytical
Analytical
Analytical
Microbore
Microbore
Analytical
Analytical
Microbore
Microbore
Microbore
Analytical
Analytical
Analytical
Analytical
Analytical
Microbore
Analytical
Microbore
Microbore
Analytical
Analytical
Analytical
Microbore
Analytical
Microbore
Microbore
Analytical
Analytical
2.1 x 50mm
2.1 x 150mm
4.6 x 50mm
4.6 x 150mm
2.1 x 50mm
2.1 x 150mm
2.1 x 250mm
4.6 x 50mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
2.1 x 50mm
2.1 x 150mm
4.6 x 50mm
4.6 x 150mm
2.1 x 50mm
2.1 x 150mm
2.1 x 250mm
4.6 x 50mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
2.1 x 50mm
4.6 x 50mm
2.1 x 50mm
2.1 x 150mm
4.6 x 50mm
4.6 x 150mm
4.6 x 250mm
2.1 x 50mm
4.6 x 50mm
2.1 x 50mm
2.1 x 150mm
4.6 x 50mm
4.6 x 150mm
100, 5m
PLRP-S Choice of Pore Sizes:

100 for small molecules, peptides, and nucleotides
300 for polypeptides and globular proteins
1000 for fibrous proteins
4000 for the analysis of very large biomolecules or
high-speed/high-resolution separations
100, 8m
300, 3m
300, 5m
PLRP-S HPLC Specifications
Base Material
Polystyrene Divinylbenzene
Organic Modifier
Particle Size
Pore Size
pH Range
Max. Temp.
Max. Pressure
Max. Flow Rate
0 to 100%
3, 5, 8, 10m
100, 300, 1000, 4000
113
80C
3000psig
4mL/min
300, 8m
1000, 5m
1000, 8m
Enkephalins
7253
4000, 5m
4000, 8m
4
3
1.
2.
3.
4.
5.
Methionine Enkephalin Sulphoxide

Methionine Enkephalin Lysine
Methionine Enkephalin
D-ALA2 Methionine Enkephalin
D-TRP Methionine Enkephalin
Part No.
1912-1300
1912-3300
1512-1300
1512-3300
1912-1500
1912-3500
1912-5500
1512-1500
1512-3500
1512-5500
1512-3800
1512-5800
1912-1301
1912-3301
1512-1301
1512-3301
1912-1501
1912-3501
1912-5501
1512-1501
1512-3501
1512-5501
1512-3801
1512-5801
1912-1502
1512-1502
1912-1802
1912-3802
1512-1802
1512-3802
1512-5802
1912-1503
1512-1503
1912-1803
1912-3803
1512-1803
1512-3803
PLRP-S is a polystyrene divinylbenzene copolymer designed

specifically for reversed-phase HPLC. PLRP-S columns are
extremely stable, performing over a wide pH range and at
elevated temperatures and pressures.
Packing
PLRP-S Guard Columns
Column:

Mobile Phase:

Gradient:
Flow Rate:
Detector:
10
Packing
i.d. x Length
Qty.
Part No.
PLRP-S, 5m
Guard Column Holder
3 x 5mm
2
ea
1512-1503
1310-0016
12 Min.
PLRP-S 100, 5m, 4.6 x 250mm

(Part No. 1512-5500)
A: 0.1% TFA, 1% IPA in Water
B: 0.1% TFA, 1% IPA in ACN
Linear 15 85% B in 15min
1.0mL/min
UV at 220nm
more info
For more large molecule columns, see pages 84 97.
related product

See page 1617.
6219
79
analytical hplc
Shodex Columns
Aqueous Size Exclusion
Aqueous Size Exclusion Columns
High efficiency and high mass recovery

Separate biopolymers by their effective size in solution
Stable sugar GFC columns
Shodex Ohpak and Sugar columns are designed for highresolution separations of water-soluble compounds such as
organics, inorganics, oligomers, and polymers. Three different
gel filtration size exclusion materials are available.
Packing Series
Description
Applications
OHpak SB-800
Polyhydroxymethacrylate
Protein KW-800
Porous Silica Gel
Sugar KS-800
Sulfonated PS Gel
(Na+ counter ion)
General purpose GFC of

water-soluble polymers,
proteins, and enzymes
GFC of proteins,
glycoproteins, and peptides
Separation of mono, di,
oligo, and polysaccharides,
starches, and celluloses
Shodex Sugar columns use a sulfonated gel with a

sodium counter ion for separation of mono, di, oligo, and
polysaccharides, starches, and celluloses.
Proteins by GFC
Pullulan, Saccharides, and Ethanol

9162
23 4
9163
1. Glutamate Dehydrogenase (290k)

2. Lactate Dehydrogenase (142k)
3. Enolase (67k)
4. Adenylate Kinase (32k)
5. Cytochrome (12.4k)
1.
2.
3.
4.
5.
6.
4
5
Column:

Mobile Phase:
Flow Rate:
Detector:
Column Temp:
10
15 Min.
Column:

Mobile Phase:
Flow Rate:
Detector:
Column Temp:
Shodex Protein KW-803, 8.0 x 300mm

(Part No. 5749)
50mM Phosphate buffer + 0.3M NaCl, pH 7.0
1.0mL/min
ELSD
28C
Pullulan (46.7k)
Pullulan (5.3k)
Raffinose
Sucrose
Fructose
Ethanol
10
12 Min.
Shodex Sugar KS-802, 8.0 x 300mm

(Part No. 5743)
Water
1.0mL/min
RI
80C
Shodex Size Exclusion HPLC Columns

OHpak
Protein
Sugar
Particle Size
Format
Packing
MW Range
8m
8m
6m
6m
10m
13m
13m
5m
5m
7m
5m
5m
5m
7m
17m
17m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Q-801
SB-802 HQ
SB-802.5 HQ
SB-803 HQ
SB-804 HQ
SB-805 HQ
SB-806M HQ
KW-802.5
KW-803
KW-804
KS-801
KS-802
KS-803
KS-804
KS-805
KS-806
501800
504000
5010,000
50100,000
1001,000,000
5004,000,000
10020,000,000
5050,000
50150,000
100600,000
501000
5010,000
5050,000
100400,000
1005,000,000
50050,000,000
related product
Part No.
5737
5730
5731
5732
5733
5734
5736
5748
5749
5750
5742
5743
5744
5745
5746
5747
related product
Doing carbohydrate analysis?

ELSD offers greater sensitivity than RI. See pages 5 11.
80
i.d. x Length
8.0 x 500mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm
8.0 x 300mm

6219
analytical hplc
Shodex ODP2 HP Columns

High-Efficiency Polymer-Based
Reversed-Phase Column
ODP2 HP Columns Specifications
hplc columns | large molecule
Column efficiency is comparable with that of silica-based

ODS columns
Better retention of highly polar substances comparing to
ODS columns
Long column life even with high protein content samples:
ODP2 HP prevent protein adsorption that causes ODS
column degradation
Excellent peak shape using low salt mobile phase, ideal
for microbore
High pH stability
ODP2 HP series is a polymer-based

[poly(hydroxymethacrylate)] column for reversed-phase
chromatography. The efficiency of ODP2 HP is improved
over most resin-based columns, with typical theoretical plate
number >65,000 per meter.
ODP2 HP Columns
Packing
Format
i.d. x Length
ODP2 HP-4B, 5m
ODP2 HP-4D, 5m
ODP2 HP-4E, 5m
ODP2 HPG-4A, 5m
ODP2 HP-2B, 5m
ODP2 HP-2D, 5m
ODP2 HPG-2A, 5m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 50mm
4.6 x 150mm
4.6 x 250mm
4.6 x 10mm
4.6 x 50mm
4.6 x 150mm
4.6 x 10mm
Packing
Theoretical Plate Number

(per column)
Theoretical Plate Number

(per meter)
Particle Size
pH Range
i.d. x Length
ODP2 HP-4B
ODP2 HP-4D
ODP2 HP-4E
ODP2 HPG-4A
ODP2 HP-2B
ODP2 HP-2D
ODP2 HPG-2A
3500
13,000
17,000
guard column
3000
7000
guard column
70,000
86,000
68,000
guard column
60,000
46,600
guard column
5m
5m
5m
5m
5m
5m
5m
312
312
312
312
312
312
312
4.6 x 50mm
4.6 x 150mm
4.6 x 250mm
4.6 x 10mm
2.0 x 50mm
2.0 x 150mm
2.0 x 10mm
Part No.
F7622001
F7622002
F7622003
F6714010
F7622004
F7622005
F6714011
Drug in Biological Fluid

Microbore is effective for the high sensitivity analysis of drugs; however, when protein is present and enters the MS (Mass
detector), it contaminates the MS or suppresses ionization of the sample. Often pretreatment does not remove protein
thoroughly. Drugs in biological fluid are hard to analyze because protein co-elutes with the component of interest. The target
drug receives ion suppression from the protein and appears as a small peak.
ODP2 HP can separate the target drug from protein by eluting protein early. The result of barbital analysis with BSA using
microbore is shown as below. Barbital was introduced into the MS by a switching valve after BSA was eluted, and barbital
was detected without any influence of ion suppression.
Barbital
1
UV
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min.
wi
tch
MS
eS
eS
wi
tch
MS
1,800K
1,600K
1,400K
1,200K
1,000K
800K
600K
400K
200K
0
130K
120K
110K
100K
90K
80K
70K
60K
BSA 50K
40K
30K
20K
10K
0
Va
lv
130K
120K
110K
100K
90K
80K
70K
60K
50K
40K
30K
20K
10K
0
Va
lv
1,800K
1,600K
1,400K
1,200K
1,000K
800K
600K
400K
200K
0
Barbital
1
UV
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min.
more info
For other Shodex columns, including KW400, KF-400, SB-800,

KF-400, KF-600, KF-800, NH2P, Ion Exchange, affinity, and sugar
columns, visit www.discoverysciences.com.
81
analytical hplc
Waters Spherisorb Columns
All Waters columns are packed by Grace to our highest QC

standards. Spherisorb columns have 80 pores and 220m2/g
surface area. ODS-1 is unendcapped with a 6% carbon load,
and ODS-2 is endcapped with a 12% carbon load.
Spherisorb HPLC Columns

Packing
Format
i.d. x Length
ODS-1, 5m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 100mm
4.6 x 150mm
4.6 x 150mm
4.6 x 250mm
4.6 x 250mm
4.6 x 250mm
ODS-2, 3m
ODS-2, 5m
C8, 3m
C8, 5m
C6, 5m
Phenyl, 5m
Cyano, 5m
Amino, 5m
Silica, 3m
Silica, 5m
SAX, 5m
SCX, 5m
Part No.
Waters Fittings
Part No.
8441
8364
8487
8558
8545
8736
8561
8609
8571
8744
8211
8223
8689
8752
8713
8361
8739
8371
8382
8554
8389
8376
8765
8888
8443
8357
8489
8560
8547
8738
8563
8611
8573
8746
8217
8224
8691
8754
8715
8362
8741
8372
8383
8556
8391
8377
8767
8889
Beckman Ultrasphere
Columns
Low metal content for symmetrical peaks
Narrow particle size range for high efficiency
Maximum surface coverage for long column lifetimes
All Ultrasphere columns are packed by Grace to our highest
QC standards. Ultrasphere columns are highly endcapped
to reduce silanol interactions and have a narrow particle size
range for excellent resolving power.
Tricyclic Antidepressants
7739
1.
2.
3.
4.
5.
6.
7.
1
7
2 34 5
0
Column:

Mobile Phase:
Flow Rate:
Detector:
Doxepin
Desipramine
Nortriptyline
Imipramine
Amitriptyline
Trimipramine
Clomipramine
10
15 Min.
Ultrasphere C18, 5m, 4.6 x 150mm

(Part No. 235330)
10mM Triethylamine, pH2.5:Acetonitrile (71:29)
2.0mL/min
UV at 216nm
Spherisorb All-Guard Cartridges*

Packing
i.d. x Length
ODS-1, 5m
4.6 x 7.5mm
ODS-2, 5m
4.6 x 7.5mm
C8, 5m
4.6 x 7.5mm
C6, 5m
4.6 x 7.5mm
Phenyl, 5m
4.6 x 7.5mm
Cyano, 5m
4.6 x 7.5mm
Amino, 5m
4.6 x 7.5mm
Silica, 5m
4.6 x 7.5mm
SAX, 5m
4.6 x 7.5mm
SCX, 5m
4.6 x 7.5mm
Qty.
Part No.
3
3
3
3
3
3
3
3
3
3
ea
96218
96219
96220
96221
96226
96225
96224
96227
96228
96217
80101
Ultrasphere HPLC Columns

Packing
Format
C18, 3m
C18, 5m
Analytical
Microbore
Microbore
Analytical
Analytical
Prep
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
C18-IP, 5m
C8, 5m
Cyano, 5m
more info
Silica, 5m
If you like Spherisorb, try Graces lower cost Allsphere on

page 59.
i.d. x Length
Part No.
4.6 x 75mm
2.0 x 150mm
2.0 x 250mm
4.6 x 150mm
4.6 x 250mm
10 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
244254
237390
244434
235330
235329
235328
235334
235335
235333
235332
244070
244071
235342
235341
Ultrasphere Guard Columns

Description
i.d. x Length
Qty.
Part No.
C18, 5m
C18-IP, 5m
C8, 5m
Cyano, 5m
Silica, 5m
4.6 x 45mm
4.6 x 45mm
4.6 x 45mm
4.6 x 45mm
4.6 x 45mm
ea
ea
ea
ea
ea
243536
243534
243532
243538
243530
Ultrasphere guard columns connect directly to the analytical column with a

zero dead volume connector, or a small piece of tubing with finger tight fittings.
No holder required.
82
analytical hplc
ZirChrom Columns
Better pH and thermal stability than silica-based columns
More efficient than polymer-based columns
Widest range of reversed-phase selectivities
ZirChrom Packings
Packing
Functional Group
Mode
DiamondBond-C18
PBD
Octadecylsilane
Polybutadiene
PS
Polystyrene
CARB
Graphitic Carbon
PHASE
EZ
MS
Zirconia
Reversed-Phase
Reversed-Phase &
Secondary Ionic
Reversed-Phase
(Low Retention)
Reversed-Phase
(High Retention)
Normal-Phase
Reversed-Phase
Reversed-Phase
DiamondBond-C18 ideal for microbore applications

and acidic compounds. pH range 114
ZirChrom-PBD for general purpose applications and
basic compounds; similar to ODS for non-electrolytes.
pH range 114
ZirChrom-PS ideal for highly aqueous mobile phases;
an alternative to ODS selectivity. pH range 114
ZirChrom-CARB for diastereomers/geometric
isomers; greatest difference in selectivity compared
to ODS. pH range 114
ZirChrom-Phase the bare zirconia particle. The
columns are used as a normal-phase alternative to silica.
pH range 114
ZirChrom-EZ unique selectivity with mobile phase
selectivity. pH range 110
ZirChrom-MS low column bleed makes it ideal for
microbore applications. pH range 110
ZirChrom Specifications
Pore Size
Particle Size
Surface Area
pH Range
Max. Temperature
300
3m, 5m
30m2/g
114 (EZ and MS 110)
200C
Unique selectivity on a zirconia platform

Mobile phase flexibility, including microbore
compatible buffers
Stable from pH 110
The deactivation of Lewis acid sites on the zirconia surface
allows for the chromatography of Lewis base analytes using
volatile mobile phase additives, including conventional
microbore compatible buffers such as acetate and formate.
ZirChrom HPLC Columns
Packing
Format
DiamondBond, 3m
Microbore
Microbore
Analytical
Analytical
Analytical
Microbore
Microbore
Analytical
Analytical
Analytical
Microbore
Microbore
Analytical
Analytical
Analytical
Microbore
Microbore
Analytical
Analytical
Microbore
Microbore
Analytical
Analytical
Microbore
Microbore
Microbore
Microbore
Analytical
Analytical
Analytical
Analytical
Microbore
Microbore
Microbore
Microbore
Analytical
Analytical
Analytical
Analytical
Microbore
Microbore
Microbore
Microbore
Analytical
Analytical
Analytical
Analytical
Microbore
Microbore
Microbore
Microbore
Analytical
Analytical
Analytical
Analytical
PBD, 3m
PS, 3m
CARB, 3m
PHASE, 3m
EZ, 3m
EZ, 5m
MS, 3m
MS, 5m
i.d. x Length
Part No.
2.1 x 100mm
2.1 x 150mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
2.1 x 100mm
2.1 x 150mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
2.1 x 100mm
2.1 x 150mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
2.1 x 100mm
2.1 x 150mm
4.6 x 100mm
4.6 x 150mm
2.1 x 100mm
2.1 x 150mm
4.6 x 100mm
4.6 x 150mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
2.1 x 250mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 250mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
2.1 x 250mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 250mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
2.1 x 250mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 250mm
2.1 x 50mm
2.1 x 100mm
2.1 x 150mm
2.1 x 250mm
4.6 x 50mm
4.6 x 100mm
4.6 x 150mm
4.6 x 250mm
DB01-1021
DB01-1521
DB01-0546
DB01-1046
DB01-1546
ZR03-1021
ZR03-1521
ZR03-0546
ZR03-1046
ZR03-1546
ZR09-1021
ZR09-1521
ZR09-0546
ZR09-1046
ZR09-1546
ZR01-1021
ZR01-1521
ZR01-1046
ZR01-1546
ZR02-1021
ZR02-1521
ZR02-1046
ZR02-1546
EZ01-0521
EZ01-1021
EZ01-1521
EZ01-2521
EZ01-0546
EZ01-1046
EZ01-1546
EZ01-2546
EZ01-05215
EZ01-10215
EZ01-15215
EZ01-25215
EZ01-05465
EZ01-10465
EZ01-15465
EZ01-25465
MS01-0521
MS01-1021
MS01-1521
MS01-2521
MS01-0546
MS01-1046
MS01-1546
MS01-2546
MS01-05215
MS01-10215
MS01-15215
MS01-25215
MS01-05465
MS01-10465
MS01-15465
MS01-25465
ZirChrom Separations, Inc. produces HPLC columns based

on 3m zirconia (zirconium oxide) particles. These columns
are more rugged and durable than silica-based columns
and more efficient than polymer-based columns. ZirChrom
normal-phase and reversed-phase columns can be used
across the entire pH range (114) and are thermally stable to
200C. Additionally, zirconias silanol-free surface produces
sharp, symmetrical peaks for amines without mobile phase
modifiers.
EZ and MS HPLC Columns
83
analytical hplc
Vydac Large Molecule Columns

Separate biomolecules from small peptides to large intact proteins with the Vydac family of products which includes reversedphase, normal-phase, ion-exchange, and affinity phases. Our extensive applications library offers solid method development
guidance, and our technical experts provide insight to even the most unusual separation challenges. Whether your primary
analysis consideration is speed, MS compatibility, resolution, or recovery, we have a solution.
Column Selection for Polypeptides

The reversed-phase column for a polypeptide separation should be selected based on the hydrophobicity of the polypeptide
being chromatographed and molecular weight as a secondary consideration.
Increasing hydrophilicity
1000
Increasing hydrophobicity
C18 =
GraceAlpha
C18 = 218TP, 238TP, 218MS
Molecular Weight
Vydac has always been a trusted name in bioseparations, now, with technology acquired by Grace over the past few years,
we have expanded this expertise further. New Grace large molecule columns range from nano, capillary to micro, analytical
to preparative columns.
10,000
C8 = 208TP, 208MS
C4 = 214TP, 214MS
Diphenyl = 219TP
Ol
igo
nu
cle
oti
de
s
Ol
igo
sa
cc
ha
rid
es
An
tib
od
ies
Un
Int -den
ac
t P ature
rot d,
ein
s
Bio
>1 mol
0K ec
MW ule
s
Comments
218MS (C18)
See page 8587
Polymeric bonding highest hydrophic

interaction and unique geometric selectivity
238MS (C18)
See page 8587
Monomeric bonding offers increased peptide

interaction and generally yields higher peak
counts. Different selectivity compared to
218MS.
208MS (C8
See page 8587
Lower hydrophobicity is better for larger

molecules
214MS (C4)
See page 8587
Ideal for hydrophobic proteins, or when

minimal organic is desired
219MS (Diphenyl)
See page 8587
Lowest capacity, highly selective for proteins

with aromatic sidechains
Everest C18
See page 8889
Maximum surface coverage for highest

resolution of complex samples
ProZap
See page 9394
Sub 2m, in Expedite format optimized for

fast analysis
218TP (C18)
See page 9092
First generation media with extensive

applications library
214TP (C4)
See page 9092
First generation media with extensive

applications library
related products
Looking for large molecule prep columns?

See pages 156 161.
84
Bio
5-1 mol
0K ecu
MW les
Phase
Sim
Dig ple
(<1 ests Enz
ym
2p
ati
rot
c
ein
s)
Co
Dig mple
(>1 ests x En
2p
zy
ma
rot
tic
ein
s)
Bio
0-5 mol
K M ecu
W les
100,000
analytical hplc
Vydac MS Introduction
New Generation Columns with Unsurpassed Resolution, Sensitivity,
and Recovery
Vydac MS columns are the latest development in the ongoing effort to provide the best reversedphase HPLC columns for biomolecule. A surface treatment and propriatary bonding process gives
Vydac MS columns a unique selective not found anywhere else. A variety of reversed phases makes
this product line suitable for small peptides to large intact, undenatured proteins.
MS Specifications
7300
Phase
Base Material
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
208MS C8
214MS C4
218MS C18
238MS C18
219MS Di-Phe
Silica
Silica
Silica
Silica
Silica
Spheroidal
Spheroidal
Spheroidal
Spheroidal
Spheroidal
5m
5m
3, 5, 10, 1015m
5m
5m
300
300
300
300
300
70m2/g
70110m2/g
60110m2/g
70m2/g
70m2/g
5%
3%
8%
4%
4%
Polymeric
Polymeric
Polymeric
Monomeric
Polymeric
Yes
Yes
Yes
Yes
Yes
L7
L26
L1
L1
Unique Selectivity
Unsurpassed Resolution and Peak Symmetry
A sample of bovine fetuin, a 36kD glycoprotein, was digested

with trypsin. Some of the sample components interfere with
the peptide separation on the Competitor 1 and Competitor 2
columns and appear as a chromatographic hump with peaks
riding on top. The unique selectivity of Vydac MS columns
solves these separation problems
Vydac MS C4 columns provide the overall best performance

for hGH and desamido hGH analysis. Competitive columns
show significant, undesirable interaction of hGH with the
stationary phase.
Human Growth Hormone (hGH) Assay Columns
Transmembrane Protein p14 Columns

2
1.
2.
3.
4.
4
1 3
1. Desamido HGH
2. HGH
18.8 kD Protein
Non-myristoylated p14
Myristoylated p14
Triton X (Surfactant)
2
2
Vydac MS C4 Column
10
20
30
1
1
40
2
2
50
60 65 Min.
Columns:
ydac MS C4 214MS54, Competitor 1 C4,

V
Competitor 2 C3, Competitor 3 C4, and
Competitor 4 C4 (all 300, 5m, except 4m for
Competitor 1, 4.6 x 250mm)
Flow Rate:
1.0mL/min
Mobile Phase: A: 0.1% v/v TFA in H2O

B: 0.085% v/v TFA in Acetonitrile
Gradient Vydac, Comp. 1, and Comp. 2:

Time: 0 20 25 45

%B: 20 60 80 80
Gradient Comp. 3 and Comp. 4:

Time: 0 20 25 45 50 80

%B: 20 60 80 80 90 90
Detector:
UV at 215nm
Column Temp: Ambient, 25C
Injection Vol: 50L
2
3
2
1
2 4
41
Unique selectivity reveals peaks otherwise masked by other C18 columns

Excellent peak shape with little or no TFA
High protein recoveries make scale-up easy
20
Columns:
Mobile Phase:
Flow Rate:
Detector:
Column Temp:
Injection Vol:
40
Vydac MS C4 Column
60 Min.
All 300, 5m, 4.6 x 250mm

Isocratic, 71% 50mM Tris, pH 7.5, 29% n-propanol
0.5mL/min
UV at 220nm
45C
20L of a 1mg/mL preparation
more info
For additional protein and peptide applications, see application

section pages 429 441.
more applications

Reptilian reovirus RRV p14 sample courtesy of Drs. Roberto J. de Antueno and Roy
Duncan, Dalhousie University, Halifax, Nova Scotia.
85
analytical hplc
Vydac MS Columns
Excellent Peak Shape with Little or No TFA
Hydrophobic Proteins
It is common practice for protein and peptide separations to

include an acidic modifier such as TFA in the mobile phase.
TFA masks basic entities, reducing mixed-mode retention,
and improving peak symmetry. TFA also changes retention
and selectivity for different analytes, and its concentration
can be adjusted to optimize a separation. Unfortunately
TFA is UV absorbent and contributes background at low
UV wavelengths. Also It is especially problematic with
electrospray MS where it interferes with ion generation,
called quenching, and reduces MS sensitivity.
Transmembrane proteins are hydrophobic proteins

which bind to cell membranes and are particularly difficult to
separate. Vydac MS columns provide excellent selectivity
and peak shape for these molecules. In this case, a
hydrophobic transmembrane protein was separated from
a synthetic myristoylated derivative and other cellular
components.
Tryptic Digest of Fetuin on

300 C18 Columns
Peptides on 218MS C18 Reversed-Phase

1.
2.
3.
4.
0.6 UA
Vydac MS C18
Column
Neurotensin 1-8 (fragment)

Oxytocin
Angiotensin II
Neurotensin
Competitor 1 C18
Column
Competitor 2 C18
Column
80 Min.
0.1% TFA
2
1
0.4
3 4
0.0
5% TFA
2
2% TFA
0.2 0.0
1
0.0
1% TFA
0
Columns:

Flow Rate:
Mobile Phase:

Gradient:
Detector:
40
60
Columns:
1
20
10
20
30 Mi
Vydac C18, 5m, 4.6 x 250mm

(Part No. 218MS54)
1.5mL/min
A: 5% Acetonitrile in Water with TFA as indicated (v/v)
B: 95% Acetonitrile in Water with TFA as in A
Linear from 0 to 20% B over 20min, then to
100% B in 5min
UV at 220nm
more applications

ydac MS C18 238MS5415, Competitor 1 C18, and

V
Competitor 2 C18 (all 300, 5m, 4.6 x 150mm)
Flow Rate:
1.0mL/min
Mobile Phase: A: 0.1% v/v TFA in H2O

B: 0.085% v/v TFA acid in Acetonitrile
Gradient:
Time: 0 5 80 90 100

%B: 4 4 40 90 90
Detector:
UV at 215nm
Column Temp: Ambient, 22C
Injection Vol: 3L of a 6 g/L digest (180 g total peptide load)
The Vydac column provides better separation and recovery for a

highly hydrophobic membrane protein (RRV p14) and its fatty acid
modified (myristolyated) form, a component of a potentially new
vaccine delivery system.
High Protein Recovery for Higher Sensitive,

Ideal for Preparative Chromatography
Surface chemistry reduces adsorption of proteins for higher
recoveries and also increases mass loading.
% Recovery at Low Protein Load: Vydac MS C4 vs.

2 Competitors
Loading Amount:
1g of Cytochrome C
100
20
Competitor 2
40
Competitor 1
60
Vydac MS
% Recovery
80
Vydac MS C4 column provides more than 20% more loading of

Cytochrome C.
86
analytical hplc
Vydac MS Columns
Vydac MS Analytical Columns
Particle Size
214 MS C4
5m
50mm
1.0mm
2.1mm
3.2mm
4.6mm
214MS5105
214MS5205
214MS5405
208 MS C8
5m
1.0mm
2.1mm
3.2mm
4.6 mm
218 MS Polymeric C18
5m
1.0mm
2.1mm
3.2mm
4.6mm
238 MS Monomeric C18
5m
1.0mm
2.1mm
3.2mm
4.6mm
219 MS Diphenyl
5m
1.0mm
2.1mm
3.2mm
4.6mm
Columns
100mm
Recommended Guards
Guard Kit1
Guard Cartridge2
150mm
250mm
214MS5110
214MS5210
214MS5410
214MS5115
214MS5215
214MS5415
214MS51
214MS52
214MS53
214MS54
214GK51MS
214GK52MS
214GK54MS
214GK54MS
214GD51MS
214GD52MS
214GD54MS
214GD54MS
208MS5105
208MS5205
208MS5405
208MS5110
208MS5210
208MS5410
208MS5115
208MS5215
208MS5415
208MS51
208MS52
208MS53
208MS54
208GK51MS
208GK52MS
208GK54MS
208GK54MS
208GD51MS
208GD52MS
208GD54MS
208GD54MS
218MS5105
218MS5205
218MS5405
218MS5110
218MS5210
218MS5410
218MS5115
218MS5215
218MS5415
218MS51
218MS52
218MS53
218MS54
218GK51MS
218GK52MS
218GK54MS
218GK54MS
218GD51MS
218GD52MS
218GD54MS
218GD54MS
238MS5105
238MS5205
238MS5405
238MS5110
238MS5210
238MS5410
238MS5115
238MS5215
238MS5415
238MS51
238MS52
238MS53
238MS54
238GK51MS
238GK52MS
238GK54MS
238GK54MS
238GD51MS
238GD52MS
238GD54MS
238GD54MS
219MS5105
219MS5205
219MS5405
219MS5110
219MS5210
219MS5410
219MS5115
219MS5215
219MS5415
219MS51
219MS52
219MS53
219MS54
219GK51MS
219GK52MS
219GK54MS
219GK54MS
219GD51MS
219GD52MS
219GD54MS
219GD54MS
i.d.
A guard kit includes a holder and one guard cartridge; 2Guard cartirdge units include two guard cartridges.
Vydac MS Nano and Capillary Columns

214 MS C4
5m
208 MS C8
5m
i.d.
50mm
100mm
150mm
250mm
75m
150m
300m
500m
214MS5.07505
214MS5.1505
214MS5.305
214MS5.505
214MS5.07510
214MS5.1510
214MS5.310
214MS5.510
214MS5.07515
214MS5.1515
214MS5.315
214MS5.515
214MS5.07525
214MS5.1525
214MS5.325
214MS5.525
75m
150m
300m
500m
208MS5.07505
208MS5.1505
208MS5.305
208MS5.505
208MS5.07510
208MS5.1510
208MS5.310
208MS5.510
208MS5.07515
208MS5.1515
208MS5.315
208MS5.515
208MS5.07525
208MS5.1525
208MS5.325
208MS5.525
218MS3.07505
218MS3.1505
218MS3.305
218MS3.505
218MS5.07505
218MS5.1505
218MS5.305
218MS5.505
218MS3.07510
218MS3.1510
218MS3.310
218MS3.510
218MS5.07510
218MS5.1510
218MS5.310
218MS5.510
218MS3.07515
218MS3.1515
218MS3.315
218MS3.515
218MS5.07515
218MS5.1515
218MS5.315
218MS5.515
218MS3.07525
218MS3.1525
218MS3.325
218MS3.525
218MS5.07525
218MS5.1525
218MS5.325
218MS5.525
238MS5.07505
238MS5.1505
238MS5.305
238MS5.505
238MS5.07510
238MS5.1510
238MS5.310
238MS5.510
238MS5.07515
238MS5.1515
238MS5.315
238MS5.515
238MS5.07525
238MS5.1525
238MS5.325
238MS5.525
219MS5.07505
219MS5.1505
219MS5.305
219MS5.505
219MS5.07510
219MS5.1510
219MS5.310
219MS5.510
219MS5.07515
219MS5.1515
219MS5.315
219MS5.515
219MS5.07525
219MS5.1525
219MS5.325
219MS5.525
218 MS Polymeric C18

3m
75m
150m
300m
500m
5m
75m
150m
300m
500m
238 MS Monomeric C18
5m
75m
150m
300m
500m
219 MS Diphenyl
5m
75m
150m
300m
500m
Vydac MS Guard Cartridges
Packing
C18 Polymeric, 5m*

Capillary Guard**
C4, 5m* Capillary Guard**
Vydac MS Guard Cartridges (continued)

i.d. x Length
Qty.
Part No.
0.150 x 10mm
0.300 x 10mm
1.0 x 10mm
0.150 x 10mm
0.300 x 10mm
1.0 x 10mm
ea
ea
2
ea
ea
2
218MS5C0115
218MS5C0130
218GD51MS
214MS5C0115
214MS5C0130
214GD51MS

Packing
Qty.
Part No.

Guard Holder for 1mm Guards
ea
ea
ea
GR-3710E
GR-3710A
GCH1
87
analytical hplc
Vydac Everest Columns Introduction

High Resolution for Complex Peptide Samples
Everest columns provide exceptionally high resolution for complex peptide digest separations.
Unique selectivity and sensitivity are the result of new bonding technology that improves C18
surface coverage and deactivates residual silanols. Previously, the best 300 C18 chemistries have
had carbon coverage in the 2.8 to 3.6mol/m2 range. Everest C18 coverage is in excess of 4mol/m2
and approximates the theoretical limit based on surface area. This increased shielding of the base silica
increases column life and reduces the amount of TFA required to shield the silica.
Everest Specifications
Phase
Base Material
238EV C18 Silica
7189
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
Spheroidal
5, 10, 1015, 1520m
300
70110m2/g
6%
Monomeric
Yes
L1
Tryptic Digest of Bovine Serum Albumin
Base Peak Chromatogram of Tryptic Digest of BSA

7
Everest Column
110 peaks
Commercial Column C
94 peaks
Commercial Column D
96 peaks
Intensity (x 106 cps)
High resolution of complex peptide digests

Unique selectivity for both hydrophilic and hydrophobic peptides
Exceptional microbore sensitivity with little or no TFA in the mobile phase
5
4
3
2
1
0
10
15
20
25
30
35
40
45
50
55
60
65
70 Min.
Column: Vydac Everest C18, 300, 5m, 4.6 x 150mm

Commercial 300 c18 columns 4.6 x 150mm

(Part No. 238EV5415)
Mobile Phase: A: 0.1% TFA in Water

Gradient:
Time: 0 5 80 90 100

%B: 4 4 40 90 90
Flow Rate:
1.0mL/min
Detector:
UV at 215nm
Everest columns outperform competitor columns at higher peptide
loads, by providing higher-resolution separations (average of 17%
higher peak counts than competitor columns tested).
15
Column:

Mobile phase:

Gradient:

Flow Rate:
Column Temp:
Detector:
25
35
45
55
65
75
85
95 Min.
Vydac Everest C18, 300, 5m, 300m x 150mm;

(Part No. 238EV5.315)
A: 0.1% v/v Formic Acid in Water
B: 0.1% v/v Formic Acid in Acetonitrile
Time: 0 5 75 85 95
%B: 4 4 40 90 90
5L/min
22C
MS + ESI
The Everest column performs exceptionally well with no TFA in the

mobile phase, ensuring excellent microbore sensitivity.
Everest 300 C18

Particle Size
i.d.
Columns
100mm
50mm
Everest Analytical Columns 238EV C18

5m
1.0mm3
238EV5105
2.1mm3
238EV5205
4.6mm3
238EV5405
Everest Capillary Columns 238ZU C18
5m
75m
238EV5.07505
150m
238EV5.1505
300m
238EV5.305
500m
238EV5.505
Recommended Guards
Guard Kit1
Guard Cartridge2
150mm
250mm
238EV5110
238EV5210
238EV5410
238EV5115
238EV5215
238EV5415
238EV51
238EV52
238EV54
238GK51EV
238GK52EV
238GK54EV
238GD51EV
238GD52EV
238GD54EV
238EV5.07510
238EV5.1510
238EV5.310
238EV5.510
238EV5.07515
238EV5.1515
238EV5.315
238EV5.515
238EV5.07525
238EV5.1525
238EV5.325
238EV5.525
A guard kit includes a holder and one guard cartridge. 2Guard cartirdge units include two guard cartridges. 3Titamium frits are standard in column diameters 4.6mm and smaller.
Everest Guard Cartridges

Packing
C18, 5m* Capillary Guard**
Everest Guard Cartridges (continued)

i.d. x Length
Qty.
Part No.
0.150 x 10mm
0.300 x 10mm
1.0 x 10mm
ea
ea
2
238EV5C0115
238EV5C0130
238GD51EV

88
Packing
Qty.
Part No.

Guard Holder for 1mm Guards
ea
ea
ea
GR-3710E
GR-3710A
GCH1
analytical hplc
Vydac Everest Columns

Tryptic Digest of Cytochrome C
Competit
or Column B
3
Columns:

Flow Rate:
Mobile Phase:

Gradient:

Detector:
Everest 238EV5415 and 4 competitor

300 reversed-phase columns, all C18,
5m, 4.6 x 150mm
1.0 mL/min
A: 0.1% v/v TFA in water
B: 0.085% v/v TFA in ACN
Time: 0 75 85 95
%B: 4 40 90 90
hold 90% B for 10 min
UV at 215nm
Competit
or Column D
Competit
or Column E
3
Competit
or Column F
3
Number ofWell-R
esolved
Peptid
e in Highlight
ed Are
as
7
Ever
est
Column
10 15 20 25 30 35 40 45 50 55 60 65 Mi
For a tryptic digest of Cytochrome C, an Everest column offers unique selectivity which allows the best separation of a group of hydrophilic
and hydrophobic peptides. To assess resolution, chromatograms of tryptic digests of several proteins on an Everest column and several other
commercial 300 C18 reversed-phase columns were compared. Peak counts were based on detection by automated chromatography software
with parameters set identically for all columns. In the analyses of a tryptic digest of cytochrome c, specific regions of the chromatogram were
examined for the number of peaks resolved. The Everest column demonstrated unique selectivity for both hydrophilic and hydrophobic peptides.
Tryptic Digest of Fetuin (a Glycoprotein)
Ever
est
Column
98 pe
aks
Competit
or Column A
97 pe
aks
Columns:

Flow Rate:
Mobile Phase:

Gradient:

Detector:
Everest 238EV5415 and 4 competitor

300 reversed-phase columns, all C18,
5m, 4.6 x 150mm
1.0 mL/min
A: 0.1% v/v TFA in water
B: 0.085% v/v TFA in ACN
Time: 0 5 80 90 100
%B: 4 4 40 90 90
UV at 215nm
Competit
or Column B
90 pe
aks
Competit
or Column C
95 pe
aks
Competit
or Column D
89 pe
aks
0
10
20
30
40
50
60
70
80 Mi
A tryptic digest of fetuin injected at high load on an Everest column provided higher peak counts compared to four commercial columns. Peak
numbers shown are the average of three separations on each column.
more applications

89
analytical hplc
Vydac TP Columns
Industry Standard for Polypeptide Separations

Referred in over 9000 patents, Vydac 300 TP is the industry-standard, for peptide,
protein, and large molecule separations
Polymeric bonded phases have exceptionally long column lifetime and negligible
phase leaching
Extensive applications library based on over two decades experience
7110
Vydac TP reversed-phase material consists of aliphatic groups bonded to the surface of 300 pore diameter silica. The large
pores of the 300 TP silica give polypeptide molecules complete access to the interior of the silica pores. The unique process by
which we manufacture Vydac TP silica results in high-purity, synthetic silica with carefully controlled characteristics. Vydac TP
silica is the standard that has defined large pore HPLC silica for polypeptide separations for nearly two decades.
Vydac TP Columns
Phase
Base Material Particle Shape
Particle Size
Pore Size Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
101TP Sil
201TP C18
202TP C18
208TP C8
214TP C4
218TP C18
219TP Di-Phe
238TP C18
Silica
Silica
Silica
Silica
Silica
Silica
Silica
Silica
5, 10, 1015, 1520m

5, 7, 10, 1015, 1520m
3, 5, 10m
3, 5, 7, 10, 1015, 1520m
3, 5, 7, 10, 1015, 1520m
3, 5, 7, 10, 1015, 1520m
3, 5, 7, 10, 1015, 1520m
3, 5, 7, 10, 1015, 1520m
300
300
300
300
300
300
300
300
8%
9%
5%
3%
8%
4%
4%
unbonded
Polymeric
Polymeric
Polymeric
Polymeric
Polymeric
Polymeric
Monomeric
No
No
Yes
Yes
Yes
Yes
Yes
L3
L1
L1
L7
L26
L1
L1
Spheroidal
Spheroidal
Spheroidal
Spheroidal
Spheroidal
Spheroidal
Spheroidal
Spheroidal
70110m2/g
7090m2/g
6090m2/g
60110m2/g
60110m2/g
60110m2/g
60110m2/g
60110m2/g
Vydac 218TP C18 Columns
Human Apolipoproteins
Vydac 218TP is a polymerically bonded endcapped

n-octadecyl reversed-phase based on 300 TP silica.
Applications
Stan
dard
Vydac 218TP reversed-phase columns are recommended for

the separation of:
Small polypeptides less than 40005000 MW
Enzymatic digest fragments
Natural and synthetic peptides
Multi-ring compounds
Specific examples include:
Tryptic digests
S. aureus V8 digests
Synthetic peptides
Natural peptides
Peptide studies
7
6
45
1 23
10
15
11
10
9
20
25
30
1. apoC-IIIa
2. apoC-IIIb
3. apoC-IIa
4. apoC-IIIc
5. apoC-I
6. apoC-IIb
7. apoA-Ia
8. apoA-Ib
9. apoA-IIc
10. apoA-IIa
11. apoA-IIb
The standard was
human insulin
35 Mi
Column:
Vydac 218TP54, C18, 5m, 4.6 x 250mm
Flow Rate:
1.2mL/min
Column Temp: 50C
Mobile Phase: A: 25% ACN/0.1% TFA in Water
B: 58% ACN/0.1% TFA in Water
Gradient:
0100% B in 33min
Vydac 208TP C8 Reversed-Phase
Separation of a Tryptic Digest on 208TP54

Reversed-Phase Column
Vydac 208TP is a polymerically bonded endcapped n-octyl

reversed-phase based on 300 TP silica.
Applications
Vydac 208TP reversed-phase columns are recommended for
the separation of:
Polypeptides up to 10,00020,000 MW
Enzymatic digest fragments
Natural and synthetic peptides
0
10
Column:
Flow Rate:
Gradient:
90
20
30
40
50
60 Mi
Vydac 208TP54, C8, 5m, 4.6 x 250mm

1.0mL/min
030% ACN with 0.1% aqueous TFA over 60min
analytical hplc
Vydac 214TP C4
Insulin Variants
Vydac 214TP is a polymerically bonded endcapped

n-butyl reversed-phase based on 300 TP silica. 214ATP is
a less extensively endcapped C4 that has been found more
suitable for resolution of degradation products in analysis of
biosynthetic human growth hormone.
2
1
5
4
3
Vydac 219TP is a polymerically bonded endcapped diphenyl

reversed-phase based on 300 TP silica. It combines
moderate retentivity with unique selectivity.
Applications
Polypeptides with aromatic side chains
Large, hydrophobic proteins
Membrane-spanning peptides
Lipid peptides
Fusion proteins from inclusion bodies
10
Column:
Gradient:
more info
For more protein and peptide applications, see the application

section pages 429 441.
1. H
2. TH6
1
218TP
(C18)
2
2
214TP
(C4)
219TP
(diphenyl)
10

15
20
25
30 Min
Vydac 218TP5415 (C18), 214TP5415 (C4) and

219TP5415 (diphenyl), all 5m, 4.6 x 150mm
1090% ACN with 0.1% TFA over 30min
18-residue helical peptide (1) and six-helix template
assembled synthetic protein (2)
Synthetic Peptides
13,14
218TP54
11
,12
polym
eric C18 89
10 1516
24
7
1
356
238TP54
14
monomeric C18 8
911 1516
13
4,5
12
10
2
1
6 7
3
0
more applications
25 Mi
Comparison of Polypeptide Separations on C18, C4,

and Diphenyl Reversed-Phase Columns
Vydac 238TP C18 Reversed-Phase
Monomerically bonded C18 provides an alternative to

218TP polymeric C18 with subtle differences in selectivity.
The combination of these adsorbents can reveal analytes that
may be hidden on a single C18 column.
20
Vydac C4, 5m, 4.6 x 250mm (Part No. 214TP54)

2730% ACN with 0.1% TFA over 25min
Columns:

Gradient:
Sample:

Applications
15
From J. Rivier and R. McClintock, J. Chrom. 268, 112-119 (1983).
Vydac 238TP is a monomerically bonded endcapped

n-octadecyl reversed-phase based on 300 TP silica.
Chicken
Bovine
Ovine
Rabbit
Human
Porcine
Rat I
Rat II
Vydac 219TP Diphenyl Reversed-Phase
1.
2.
3.
4.
5.
6.
7.
8.
Applications
Glycoproteins
Hemoglobin variants
Histones
Human growth hormone
Insulin variants
Membrane proteins
10
Columns:

Flow Rate:
Gradient:
Detector:
15
20
25
1. GY
2. VYV
3. Neurotensin fragment
1-8 (pELYENKPR)
4. Ac-RGGGGLGLGK-NH2
5. RGAGGLGLGK-NH2
6. Ac-RGAGGLGLGK-NH2
7. Ac-RGVGGLGLGK-NH2
8. Oxytocin (CYIQNCPLG-NH2)
9. Met Enkephalin (YGGFM)
10. Bradykinin (RPPGFSPFR)
11. Ac-RGVVGLGLGK-NH2
12. Neurotensin Fragment
8-13 (RRPYIL)
13. Angiotensin II (DRVYIHPF)
14. Leu Enkephalin (YGGFL)
15. Neurotensin
(pELYENKPRRPYIL)
16. Angiotensin I
30 Mi (DRVYIHPFHL)
Vydac 218TP54 and 238TP54, both C18, 5m,

4.6 x 250mm
1.0mL/min
1040% ACN with 0.1% TFA (w/v) over 30min
UV at 220nm
91
analytical hplc
Vydac TP Columns
Vydac TP Analytical Columns
Particle Size
i.d.
50mm
4.6mm
1.0mm
2.1mm
3.2mm
4.6mm
218TP3405
218TP5105
218TP5205
218TP5305
218TP5405
4.6mm
1.0mm
2.1mm
3.2mm
4.6mm
4.6mm
1.0mm
2.1mm
3.2mm
4.6mm
214ATP C4 Columns
5m
2.1mm
4.6mm
219TP Diphenyl
3m
4.6mm
5m
1.0mm
2.1mm
3.2mm
4.6mm
238TP C18
3m
4.6mm
5m
1.0mm
2.1mm
3.2mm
4.6mm
218TP C18
3m
5m
208TP C8
3m
5m
214TP C4
3m
5m
Columns
100mm
Recommended Guards
Guard Kit1
Guard Cartridge2
150mm
250mm
218TP3410
218TP5110
218TP5210
218TP5310
218TP5410
218TP5115
218TP5215
218TP5315
218TP5415
218TP51
218TP52
218TP53
218TP54
218GK34
218GK51
218GK52
218GK54
218GK54
218GD34
218GD51
218GD52
218GD54
218GD54
208TP3405
208TP5105
208TP5205
208TP5305
208TP5405
208TP3410
208TP5110
208TP5210
208TP5310
208TP5410
208TP5115
208TP5215
208TP5315
208TP5415
208TP51
208TP52
208TP53
208TP54
208GK34
208GK51
208GK52
208GK54
208GK54
208GD34
208GD51
208GD52
208GD54
208GD54
214TP3405
214TP5105
214TP5205
214TP5305
214TP5405
214TP3410
214TP5110
214TP5210
214TP5310
214TP5410
214TP5115
214TP5215
214TP5315
214TP5415
214TP51
214TP52
214TP53
214TP54
214GK34
214GK51
214GK52
214GK54
214GK54
214GD34
214GD51
214GD52
214GD54
214GD54
214ATP52
214ATP54
219TP3405
219TP5105
219TP5205
219TP5305
219TP5405
219TP3410
219TP5110
219TP5210
219TP5310
219TP5410
219TP5115
219TP5215
219TP5315
219TP5415
219TP51
219TP52
219TP53
219TP54
219GK34
219GK51
219GK52
219GK54
219GK54
219GD34
219GD51
219GD52
219GD54
219GD54
238TP3405
238TP5105
238TP5205
238TP5305
238TP5405
238TP3410
238TP5110
238TP5210
238TP5310
238TP5410
238TP5115
238TP5215
238TP5315
238TP5415
238TP51
238TP52
238TP53
238TP54
238GK34
238GK51
238GK52
238GK54
238GK54
238GD34
238GD51
238GD52
238GD54
238GD54
NOTE: Additional column diameters and lengths are available on request. Please contact Grace Davison Discovery Sciences to discuss your requirements.
1
A guard kit includes a holder and one guard cartridge; 2Guard cartirdge units include two guard cartridges.
related products
For prep Vydac TP Columns, see our prep section

pages 158 160.
related products
related products
Need high-pressure
polymeric fittings?
See pages 112114
for our full selection of
high-pressure fittings.
Looking for HPLC column

prefilters? See page 111.
6673


related products
Need HPLC tubing?
See pages 384 391.
5535
92
analytical hplc
Vydac ProZap C18
46
Ultra Fast Protein and Peptide Separations

10 times faster bioseparations than traditional column formats
12 5
Ultra-high efficiency 1.5m packing
Fast protein and peptide separations with conventional HPLC systems
7279
Optimization of Gradient Parameters for Fast Protein Analysis
Short ProZap columns are the perfect tool for fast reversed-phase protein separations. Under gradient conditions, longer
6
3
columns only increase run time and do not increase resolution to improve the separation.
Proteins adsorb at the head of the
7
column and then desorb once the critical mobile phase concentration is reached. Since the proteins
do not interact with the full
2 proteins of broad molecular weight ranges
length of the packed bed, short columns are sufficient for full resolution. Therefore,
1
5
can be separated in less than one minute by combining short columns, higher flow rates, and fast, modified gradients. For best
results high-pressure mixing should be used with fast gradients.
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min.
ProZap C18 Specifications

Phase
Base Material
C18 ProZap Silica
Particle Shape
Particle Size
Pore Size
Surface Area
Carbon Load
Phase Type
Endcapped?
USP L-code
Spherical
1.5m
500
59m2/g
3%
Monomeric
Yes
L1
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min.
ProZap 1.5m, 500 packings are ideal for fast bioseparations in life science applications. The
combination of ProZap packings and short, 10mm Expedite column hardware delivers not only
faster sample throughput, but also low back pressures suitable for conventional LC systems.
Proteins
33
6
44 6
1.
2.
3.
4.
5.
6.
7.
77
11 22 55
Ribonuclease (37ng)
Bovine Insulin (37ng)
Cytochrome C (37ng)
Lysozyme (4ng)
Bovine Serum Albumin (37ng)
Carbonic Anhydrase (37ng)
Ovalbumin (37ng)
4.5 Min.
Min.
0.0
0.0 0.5
0.5 1.0
1.0 1.5
1.5 2.0
2.0 2.5
2.5 3.0
3.0 3.5
3.5 4.0
4.0 4.5
Flow Rate:
0.8mL/min
Backpressure: 185bar = 2680psig
Gradient:
Time: 0 1.0 1.1 1.2

%B: 23 75 75 23
2
11 2
3
2
1
7
5
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min.
44
6
6
33
7
7
5
5
0.0
0.5 1.0
1.0 1.5
1.5 2.0
2.0 2.5
2.5 3.0
3.0 3.5
3.5 4.0
4.0 4.5
4.5 Min.
Min.
0.0 0.5
Flow Rate:
0.4mL/min
Backpressure: 95bar = 1380psig
Gradient:
Time: 0 4.0 4.5 4.7

%B: 23 75 75 23
Flow Rate:
Backpressure:
Gradient:

0.2mL/min
45bar = 650psig
Time: 0 4.0 4.5 4.7
%B: 23 75 75 23
Column:
HPLC System:
Mobile Phase:

Detector:
Injection Vol:
Injection Amt:
Column Temp:
ProZap C18, 2.1 x 10mm, C18, 1.5m, 500

High-pressure gradient, binary pump
UV at 280nm
5L
37ng each protein, except 4ng for lysozyme
25C
Seven proteins were tested on a 10mm ProZap column. By increasing flow rate and reducing gradient time, total run time is reduced from
4.5 minutes to 1.2 minutes.
44
33
66
77
2
For more information2 about 55the Expedite hardware format,
1
see page 31. 1
more info
more applications

0.0
0.0 0.5
0.5 1.0
1.0 1.5
1.5 2.0
2.0 2.5
2.5 3.0
3.0 3.5
3.5 4.0
4.0 4.5
4.5 Min.
Min.
6113
93
analytical hplc
Vydac ProZap Columns

Antibodies in Sheep Serum
ProZap vs. Ion Exchange
Separation of Antibodies in Sheep Serum
1. IgG
2. Albumin
1. Albumin
2. IgG
1
0
Column:
HPLC System:
Mobile Phase:

Gradient:

Flow Rate:
Detector:
Injection Vol:
Column Temp:
Backpressure:
6 Min.
ProZap C18, 2.1 x 10mm, C18, 1.5m, 500

B: 0.085% TFA in 90:10 n-propanol:water
Time: 0.0 6.0 6.5 7.0
%B: 5 75 75 5
0.5mL/min
UV at 280nm
5L
75C
170bar = 2465psig
11
Vydac ProZAP columns separate intact IgG antibodies (150 kDa)

rapidly. Other traditional HPLC methods, such as ion exchange take
longer and typically involve the use of non-volatile buffers.
Synthetic Peptides10 Injections
1.
2.
3.
4.
5.
15 Mi
Column:
DEAE-type anion exchange, 7.5 x 50mm)
Mobile Phase: 10mM HEPES/TEA, pH 8.0
Gradient:
0 to 0.5 M NaCl in 20min
Peptides
13
GY (238 Da)
VYV (379 Da)
Met Enkephalin (YGGFM, 573 Da)
Leu Enkephalin (YGGFL, 555 Da)
Angiotensin II (DRVYIHPF, 1045 Da)
23
0.6
0.8
1.
2.
3.
4.
5.
Contaminant peptides/impurities
RGAGGLGLGK-amide (883 Da)
Ac-RGAGGLGLGK-amide (926 Da)
Ac-RGVGGLGLGK-amide (954 Da)
Ac-RGVVGLGLGK-amide (996 Da)
0.2 0.4 0.6 0.8 1.0 1.2 Min.
Column:
HPLC System:
Mobile Phase:

Gradient:

Flow Rate:
Detector:
Injection Vol:
Injection Amt:
Column Temp:
Backpressure:
ProZap C18, 2.1 x 10mm, C18, 1.5m, 500

B: 0.085% TFA in 80:20 Acetonitrile:Water
Time: 0.0 0.1 0.7 1.1 1.2
%B: 4 15 20 50 4
0.8mL/min
UV at 215nm
5L
600ng each, except 200ng for GY
25C
180bar = 2610psig
Peptides under one minute.
0.2
0.4
Column:
HPLC System:
Mobile Phase:

Gradient:

Flow Rate:
Detector:
Injection Vol:
Injection Amt:
Column Temp:
Backpressure:
1.0 Min.
ProZap C18, 2.1 x 10mm, C18, 1.5m, 500

B: 0.085% TFA in 80:20 Acetonitrile:Water
Time: 0.0 0.1 0.7 1.1 1.2
%B: 4 15 20 50 4
0.8mL/min
UV at 215nm
5L
500ng each
25C
180bar = 2610psig
Reproducibly separate synthetic peptides can be in one minute.
ProZap (1.5m)
94
Dimension
2.1 x 10mm
2.1 x 20mm
4.6 x 10mm
4.6 x 20mm
7 x 33mm
Part No.
35585
35587
35586
35588
35589
analytical hplc
Protein A Affinity Columns for Antibody

Purification
The Venture A column is the first affinity column to take

advantage of silicas rigid porous structure, providing
increased productivity over other supports. Graces
patented technology, ICEtech (inert coating enhancement
technology), eliminates non-specific binding on the silica
surface enabling the breakthrough use of silica for affinity
separations.
20mM Phosphate and 0.15M NaCl Buffer pH 7.4 was loaded

on each column up to 90% of the total capacity. After loading
the columns were washed with phosphate buffer and the IgG
eluted with citrate buffer pH 3.2.
90
88
86
84
82
80
78
76
Venture A
Venture A columns use a recombinant protein A ligand for

binding antibodies. They are designed to perform both as an
analytical tool for fast, accurate measurement of antibody
titers and as a quick and easy way to purify antibodies from
cell culture supernate, serum, or other feedstocks.
Venture A Characteristics
Composition:
Wide-porous spherical silica treated with
ICEtech surface passivation technology
Particle Size:
1520m
Ligand:
Recombinant protein A
pH Range:
pH 1 to 8 (long term), pH 1 to 9 (short term)
Pressure:
Maximum operating pressure =
3000psig (200bar)
Recommended
500cm/h
Mobile-Phase Velocity:
Mobile-Phase
1505000cm/h
Velocity Range:
Delivery Conditions:
20% ethanol
Storage:
At +4 to +8C in 20% ethanol
Chemical Stability:
Stable in all aqueous buffers with pH 1 to 8
commonly used in protein A chromatography
Static Capacity of Venture A Affinity Columns

Polyclonal
Species
Capacity (mg/mL)
human
bovine
mouse
rabbit
sheep
Monoclonal
40
23
38
37
13
Subclass
Capacity (mg/mL)
IgG1 Kappa
IgG2a
IgG2b
15
29
23
Column A
Column B
Column C
IgG Purification from Bovine Serum
High binding capacity for polyclonal and monoclonal

antibodies
No non-specific binding patented ICEtech (Inert
Coating Enhancement Technology, U.S. Patent No.
6,802,966) completely passivates the silica surface to
ensure highly purified antibodies
Long lifetimeproven over 300 runs
Standard HPLC column format uses biocompatible PEEK
hardware for use with HPLC or FPLC instruments
High Recovery: Experience Up to a 10%

Improvement in Recovery Compared to Other
Commercially Available Columns, Increasing Your
Yield and Measurement Accuracy
% Recovery
Vydac Venture A
2
1. Serum Load Passthrough
2. IgG
15
25
35
45 Mi
Column:
enture A RAVE1520405 (Recombinant

V
Protein A, 15 20m, 4.6 x 50mm)
Flow Rate:
1.38mL/min (linear velocity = 500cm/hr)
Mobile Phase: A: 20mM phosphate, pH 7.4, 150mM NaCl

B: 0.1M sodium citrate, pH 3.2
Gradient:
Equilibrate with A for 3min, load sample for 9min,
wash with A for 12min, elute with B for 3min,
re-equilibrate with A for 6min
Detector:
UV at 280nm
Venture A Affinity Columns

Particle Size
i.d.
50mm
100mm
15 20m
2mm
4.6mm
7.5mm
RAVE1520205
RAVE1520405
RAVE1520410
RAVE1520710
NOTE: Additional column diameters and lengths are available on request. Please
contact Grace Davison Discovery Sciences to discuss your requirements.
related products
Venture Immunoaffinity columns are also developed for small molecules, which have been fully validated. Applications areas include:
Food (Aflatoxin, Lactoferrin, Vitamin B12, Testosterone, Nortestosterone), Endocrine Disruptors (17-Ethynyl Estradiol, 17-Estradiol,
Estrone, Bisphenol A) and Pollutants (Chlorophenoxy Acetic Acid Herbicides, Phenylurea Herbicides, Organophosphorus Pesticides,
Vinclozolin Fungicide). For details, visit www.discoverysciences.com.
95
analytical hplc
Alltech ProSphere HP 300 Columns
High recoveries at low sample concentrations and

low TFA concentrations
Use for protein, peptides, sequencing, nucleic acid
fragments
pH range 27.5
ProSphere HP 300 HPLC Columns

Packing
Format
i.d. x Length
Part No.
C18, 3m*
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Solvent Reducer
Solvent Reducer
Analytical
Analytical
Solvent Reducer
Solvent Reducer
Analytical
Analytical
Capillary
Capillary
Capillary
Capillary
Capillary
Capillary
Solvent Reducer
Solvent Reducer
Analytical
Analytical
Solvent Reducer
Solvent Reducer
Analytical
Analytical
0.150 x 50mm
0.150 x 150mm
0.150 x 150mm
0.300 x 50mm
0.300 x 100mm
0.300 x 150mm
3.0 x 50mm
3.0 x 150mm
4.6 x 50mm
4.6 x 150mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
0.150 x 50mm
0.150 x 150mm
0.150 x 150mm
0.300 x 50mm
0.300 x 100mm
0.300 x 150mm
3.0 x 50mm
3.0 x 150mm
4.6 x 50mm
4.6 x 150mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
35510
35423
35516
35511
35424
35517
35512
35518
35513
35520
35519
35522
35521
35523
35533
35464
35539
35534
35465
35545
35535
35547
35536
35549
35548
35561
35560
35562
These columns produce sharp symmetrical peaks even

for basic biomolecules. The 300 pores allow access of
large proteins while still maintaining high resolution of
small peptides.
C18, 5m
A C4 Competitive Comparison
10466
C4, 3m*
10467
1.
2.
3.
4.
5.
5
3
Ribonuclease A (0.2mg/mL)
Insulin (0.067mg/mL)
Lysozyme (0.2mg/mL)
Myoglubin (0.2mg/mL)
Ovalbumin (0.4mg/mL)
C4, 5m
A. ProSphere HP C4
B. Leading Competitive C4
0
10
15
20
25
*5m, 10m particles and other dimensions are also available.
30 Min.
ProSphere HP 300 All-Guard Cartridges*
Column:
C4, 5m, 300, 4.6 x 150mm (Part No. 35560)
Mobile Phase: A: 0.01%TFA in Water
B: 0.01%TFA in Acetonitrile
Gradient:
Time: 0 40

%B: 20 100
Flow Rate:
1.0mL/min
Detector:
UV at 220nm
Injection Vol.: 10L
Packing
i.d. x Length
Qty.
Part No.
300 C18, 5m
3.0 x 7.5mm
4.6 x 7.5mm
0.150 x 10mm
0.300 x 10mm
3.0 x 7.5mm
4.6 x 7.5mm
0.150 x 10mm
0.300 x 10mm
3
3
3
3
ea
35526
35527
22668
22669
35565
35566
22678
22679
80101
ea
ea
GR-3710E
GR-3710A
HP C18, 3m
Capillary Guard**
300 C4, 5m*
HP C4 Capillary Guard**
Even at very low concentrations of TFA, ProSphere HP C4 exhibits

excellent recoveries; significantly better than a leading competitive
C4 column.

**5m, 10m particles and other dimensions are also available.
Table 1Percent Protein Recovery at Low Sample Concentration

Phase
ProSphere HP C18
A Leading Competitive C18
ProSphere HP C4
A Leading Competitive C4
Insulin
0.02mg/mL
0.4mg/mL
68
13
79
31
94
90
95
93
Ribonuclease A
0.02mg/mL
0.4mg/mL
74
0
79
53
94
84
94
91
Lysozyme
0.02mg/mL
0.4mg/mL
45
0
64
20
87
74
92
75
Bovine Serum Albumin

0.02mg/mL
0.4mg/mL
65
0
74
0
90
63
93
53
Human Serum Albumin

0.02mg/mL
0.4mg/mL
66
0
72
0
Every column has good recoveries with high sample concentrations. How does your column compare at low sample concentrations?
ProSphere HP 300 phases outperform a leading competitor at 0.02mg/mL.
related products
Looking for ProSphere prep columns?

See page 161.
96
87
63
89
68
analytical hplc
Alltech ProSphere
HP C18-AQ Columns
Alltech Macrosphere
GPC Columns
Ideal for Peptide Mapping
ProSphere HP C18-AQ Specifications

Base Material
Silica
Particle Size
3m, 5m
Pore Size
100
pH Range
28
Macrosphere GPC Exclusion Limits in Daltons

Pore Size
Format
i.d. x Length
GPC 60
Analytical
Semi-Prep
Analytical
Semi-Prep
Analytical
Semi-Prep
Analytical
Semi-Prep
4.6 x 250mm
7.5 x 300mm
4.6 x 250mm
7.5 x 300mm
4.6 x 250mm
7.5 x 300mm
4.6 x 250mm
7.5 x 300mm
10492
GPC 150
10493
GPC 300
A. C18-AQ
B. Competitive C18
Column:
Mobile Phase:

Gradient:

Flow Rate:
Detector:
Column Temp:
20
30
40
50 Min.
Format
C18-AQ, 5m
i.d. x Length
Part No.
150m x 50mm
150m x 150mm
300m x 50mm
300m x 150mm
3.0 x 50mm
3.0 x 150mm
4.6 x 50mm
4.6 x 150mm
3.0 x 150mm
3.0 x 250mm
4.6 x 150mm
4.6 x 250mm
35568
35574
35569
35575
35570
35576
35571
35578
35577
35580
35579
35581
ProSphere HP C18-AQ All-Guard Cartridges*

Packing
i.d. x Length
3.0 x 7.5mm
4.6 x 7.5mm
C18-AQ, 5m
88129
88175
88133
88177
88137
88179
88141
88181
88130
88134
88138
88142
Macrosphere GPC All-Guard Cartridges*

Packing, 5m
i.d. x Length
GPC
4.6 x 7.5mm
Qty.
Part No.
3
ea
96133
80101
300 weak and strong ion-exchange phases separate

proteins, enzymes, and other biologicals
ProSphere HP C18-AQ HPLC Columns

Capillary
Capillary
Capillary
Capillary
Solvent Reducer
Solvent Reducer
Analytical
Analytical
Solvent Reducer
Solvent Reducer
Analytical
Analytical
Waters Fittings
Part No.
Alltech Macrosphere
Ion-Exchange Columns
This water-wettable phase is ideally suited for LC/MS and delivers

more peaks than traditional C18 phases.
C18-AQ, 3m
Standard
Part No.
5m, 4.6 x 150mm (Part No. 35579)

A: 5mM Acetic Acid in Water
B: 5mM Acetic Acid in Acetonitrile
Time: 0 50
%B: 0 40
0.8mL/min
UV at 220nm
35C
Packing
28,000
350,000
500,000
1,200,000
Packing
GPC 100
10
Excluded
250
2500
4000
7500
Macrosphere GPC Columns
Tryptic Digest of Cytochrome C
Included
60
100
150
300
Stable from 100% aqueous to 100% organic

mobile phases
Compatible with a broad range of LC/MS compatible
mobile phases
7m GPC phases separate proteins, peptides, and polar

synthetic compounds by size-exclusion
Qty.
Part No.
3
3
ea
35582
35583
80101
Macrosphere Ion-Exchange Specifications

WAX
WCX
SAX
SCX
Exchange Group
Exchange Capacity
Diethylaminoethane
Carboxylic Acid
Quaternary Ammonium
Sulfonic Acid
0.13 meq/gram
0.49 meq/gram
0.14 meq/gram
0.50 meq/gram
Macrosphere Ion-Exchange Columns

Packing
Format
i.d. x Length
Part No.
WAX, 7m
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
4.6 x 150mm
4.6 x 250mm
71151
71154
71157
71160
72303
72603
72308
72608
WCX, 7m
SAX, 7m
SCX, 7m
Macrosphere Ion-Exchange All-Guard Cartridges*

Packing
i.d. x Length
WAX, 5m
4.6 x 7.5mm
WCX, 5m
4.6 x 7.5mm
SAX, 5m
4.6 x 7.5mm
SCX, 5m
4.6 x 7.5mm
Qty.
Part No.
3
3
3
3
ea
96135
96137
96139
96141
80101
97
analytical hplc
Custom HPLC Columns

Ordering Instructions
hplc columns | custom
Alltech offers a large selection of packing materials and formats to create

your own custom HPLC column.
Step 1: Select your packing material and particle size from Table 1.
Step 2: Select your column format from Table 2.
Step 3: Call your local Customer Service office for a quote. Indicate phase,
particle size, format, and dimensions.
Table 1HPLC Packing Materials
Family
Phase
Particle Sizes
Adsorbosphere
C18, C8, CN, NH2, Silica, HS C18, HS Silica, UHS C18, Phenyl, CN-AQ, SAX, SCX, OPA HR, OPA
HS, CN-AQ, SAX, SCX, OPA HR, OPA HS
C18 300, C8 300, C18, C8, Silica, SAX, SCX, TMS
C8, CN, NH2, ODS-2, Silica, C1, ODS-1, Phenyl, C6, SAX, SCX
C18, C8, Silica, CN, NH2, Phenyl, C18-LL, C18-WP, C4-WP
C18, C18 Amide, C18 EPS, C18 HiLoad, C8, CN, Silica
C18, C8, Phenyl, Silica
C18 BDS, C18 ODS, C8, Phenyl, Cyano BDS, Cyano, Amino, Silica
Anomer, NH2
C18, C8, Silica, CN, NH2
C18
C18, C8, Silica
ODS-0, ODS-1, ODS-3, ODS-4, ODS-5, ODS-6, ODS-7, C8, C6, C4, C1, CN, NH2, DIOL, IEX, Silica
C18, C8, C4, Silica
Transition Metal, Universal Cation, Surfactant C8, Surfactant C18, Surfactant/R, Allsep, Allsep
A-2, Novosep A-1
C18, C4, C8, NH2, Silica
NH2, RP-18, RP-8, RP-Select B, Silica-60, Silica-100
RP-18, RP-18 Endcapped, RP-8, RP-8 Endcapped, Silica-60, CN, Diol, NH2, Silica-100, RP-Select B
GPC 60, 100, 150, 300, SAX 300, SCX 300, WAX 300, WCX 300
C18/Cation, C8/Anion, C8/Cation
C18, C8, CN, NH2, SA, SB, Silica, Phenyl
C8, ODS-2, ODS-3, PAC, SAX, Silica, ODS, SCX
C18-300, EPS C18-300, Silica-300, C18, EPS C18, C8, C18, EPS C18, C8, EPS C8, CN, NH2,
Phenyl, Silica, SAX
Amide, Amino, C18, C18 Select, C8, CN, Phenyl, Silica
C18 300, C18 100, C4 300
C8, CN, NH2, ODS-2, Silica, ODS-1, Phenyl, C6, SAX, SCX
C18, C8, C4, Diphenyl
C18, C8, C4, Diphenyl
3, 5, 10m
Adsorbosphere XL
Allsphere
Alltima
Alltima HP
Apollo
Brava
Carbohydrate
Econosphere
Everest
GraceAlpha
Grom-Sil
Grom-Sapphire
Ion Chromatography
Kromasil
LiChrosorb
LiChrospher
Macrosphere
Mixed-Mode
Nucleosil
Partisil
Platinum
Prevail
ProSphere HP
Spherisorb
Vydac MS
Vydac TP
Table 2Custom Part Numbers for HPLC Column Formats

Format
Capillary
Custom Part No. Description
Call
Analytical, SolventReducer, and LC/MS

Analytical, Waters
Style Endfittings
Metal-Free*
C-6000M
Rocket
C-6000R
All-Guard Cartridges
Stainless Steel
All-Guard Cartridges
Metal-Free*
Preparative**
Capillary Guards
C-6000B
C-6000C
C-9800
C-9400
C-6000F
Call
Quartz glass Grace capillary columns connect directly to

microinjector and via fused silical capillary detectors
Stainless steel analytical column with industry standard
female fittings.
Stainless steel analytical column with Waters-style
endfittings.
Metal-Free columns with industry standard female
fittings.*
Fast-analysis columns with Rocket endfittings;
requires 3m or smaller particle size.
4/pk All-Guard Cartridges, Stainless Steel. Purchase
holder 80101 separately.
4/pk All-Guard Cartridges, PEEK. Purchase holder
80101 separately.
Stainless steel preparative column with threaded
endfittings.
Create a zero dead volume finger-tight connection to
capillary columns.
*Metal-Free columns are available with ProSphere and Ion Chromatography packings only.
**Additional custom prep columns available, see pages 162170.
more info
For more information on our column formats, see page 31.
106
7102a
3, 5, 10m
3, 5m
3, 5, 10m
1.5, 3, 5m
5m
3, 5m
10m
3, 5, 10m
5, 10, 1520m
5, 10, 15, 20m
1.5, 3, 5, 7, 10m
3, 5, 10m
5, 7, 10m
5, 10m
5, 10m
3, 5m
5, 7m
5, 7m
3, 5, 10m
5, 10m
1.5, 3, 5m
3, 5m
1.5, 3, 5, 10m
3, 5m
3, 5, 1015m
3, 5, 1015m
Available Lengths
Available i.d.s
5, 10, 20, 50, 75, 100,

150, 250mm
30, 50, 100, 150, 250,
300mm
30, 50, 100, 150, 250,
300mm
30, 50, 100, 150, 250,
300mm
33, 53mm
0.075, 0.1, 0.2, 0.3, 0.5, 0.8mm
2.1 (30 or 100mm lengths only),

4.6, 7.5mm
7mm
7.5mm
2.1, 3.0, 4.6mm
7.5mm
4.6mm
2.1, 3.0, 3.9, 4.1, 4.6mm

2.1, 3.0, 3.9, 4.1, 4.6mm
70, 100, 250, 500mm 7, 10, 22mm

5, 10, 20mm
0.1, 0.15, 0.3, 0.5mm
analytical hplc
Empty HPLC Hardware

Stainless Steel Analytical Column Hardware
Endfitting
Threaded tube for easy assembly

Type 316 stainless steel construction
Column i.d. of 2.1, 3.2, 3.9, 4.1, and 4.6mm
Ferrule
Compression
Screw
Frit/Insert
0765
Threaded Column Tube
Figure 1Stainless steel analytical columns have ports with industry

standard stop depth.
Endfitting
5319
Stainless steel analytical columns come complete with the

threaded tube, two 2m industry standard frit/inserts, and two
stainless steel endfitting nuts. Removable color-coded bands
available separately, (Part No. 93237). Industry standard
frit/inserts contain a stop depth equal to 0.100" (see Figure 1).
Optional 0.5m porosity frit/inserts are also available.
Adapters and sealing rings are available for slurry packing
your own columns (see Figure 2). The packing adapter
comes with a 1/4" precolumn tube to connect to 1/4" slurry
reservoirs.
Stainless Steel
Analytical Column
Packing Adapter
hplc columns | hardware
Stop Depth
(0.100")
Frit/Insert
Sealing Ring
2730
Figure 2Packing adapter threads directly onto stainless steel

analytical column tube.
Stainless Steel Analytical Column Hardware Specifications

Material:
316 Stainless Steel
Max. Temperature:
100C
Max. Pressure
10,000psig
Thread Type:
10-32 UNF (CPI Standard Ports)
Typical Use:
Analytical HPLC Columns
Stainless Steel Analytical Hardware and Accessories

Description
Complete Columns
50mm
100mm
150mm
250mm
300mm
Replacement Parts
Endfitting (ea)
Frit/Insert, 2m (5/pk)
Frit/Insert, 0.5m (5/pk)
Packing Accessories
Packing Adapter (ea)
Sealing Rings (10/pk)
2.1mm i.d.
Part No.
3.2mm i.d.
Part No.
3.9mm i.d.
Part No.
4.1mm i.d.
Part No.
4.6mm i.d.
Part No.
65175
65176
65177
65178
65179
65170
65171
65172
65173
65174
65165
65166
65167
65168
65169
65160
65161
65162
65163
65164
65150
65152
65154
65156
65158
75001
75028
75029
75001
75026
75027
75001
75024
75025
75001
75022
75023
75001
75020
75021
65189
65199
65188
65198
65187
65197
65186
65196
65185
65195
107
analytical hplc
Empty HPLC Hardware
Stainless Steel Preparative Column Hardware

Threaded format for easy assembly
Extended column life with the patented continuously
adjustable piston (CAP)
Column i.d. of 7, 10, and 22mm
Passivated stainless steel for a more inert surface

Uses precision bore tubing and OptiFlow endfittings
Column i.d. of 2.1, 3.2, 4.6, 10, and 22.5mm
The Alltech easy-to-use preparative format has threaded

endfittings and a continuously adjustable piston/frit (CAP).
The piston/frit extends column life by compressing the packed
bed to remove voids.
Column Tube
Continuously
Adjustable
Piston/Frit
Stainless Steel Swaged Column Hardware
Stainless steel swaged hardware uses precision bore

seamless tubing and OptiFlow endfittings. It is the ideal
choice when standard column lengths are not desirable
just cut to your specification. OptiFlow endfittings have a
flow distributor which spreads the sample and eluant across
the entire frit surface to improve peak symmetry and prevent
frit blockage.
Endfitting
2m
SS Frit
Distributor
Disk
Endfitting
Tubing
Nut and
Ferrule
5568
5544
Stainless Steel Preparative Column Hardware Specifications

Material:
316 Stainless Steel, PEEK
Max. Temperature:
100C
Max. Pressure
8000psig
Thread Type:
10-32 UNF (CPI Standard Ports)
Typical Use:
Prep HPLC Columns
Precision Bore HPLC Column Tubing Specifications

Material:
316 Stainless Steel
Max. Temperature:
500C
Max. Pressure
8000psig
Typical Use:
HPLC Column Assembly w/Swageable
Endfittings
Stainless Steel Preparative Hardware and Accessories

i.d.
Length
Complete Columns
7mm
100mm
7mm
250mm
7mm
500mm
10mm
100mm
10mm
250mm
10mm
500mm
22mm
70mm
22mm
100mm
22mm
250mm
22mm
500mm
Replacement Endfittings
7mm
10mm
22mm
Replacement Piston/Frit, 2m
7mm
10mm
22mm
Packing Adapters*
7mm
10mm
Packing Seals*
7mm
10mm
*22mm packing adapters and seals are not available.
108
Precision Bore Tubing
Qty.
Part No.
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
96500
96501
96502
96510
96511
96512
96524
96520
96521
96522
ea
ea
ea
96505
96515
96525
ea
ea
ea
96507
96517
96527
ea
ea
96508
96518
10
10
96509
96519
o.d. x i.d.
Precision Bore Tubing

1/8" x 2.1mm
1/4" x 2.1mm
1/4" x 3.2mm
1/4" x 4.6mm
Per Foot
Part No.
Per Meter
Part No.
3012
3013
3011
3015
30120
30130
30110
30150
OptiFlow Endfittings*
Description
OptiFlow Endfittings
1/4"
3/8"
1/2"
1"
For use with Tubing i.d.
Qty.
Part No.
2.1, 3.2, 4.6mm

7.0mm
10.0mm
2.25mm
ea
ea
ea
ea
400612
10123
9205
9204
*Each fitting is supplied with a nut and ferrule for 1/16" tubing, a 2-micron stainless steel
frit and distributor disk.
related products
Additional prep hardware also available. See page 150.
analytical hplc
Stainless Steel Frits

Type 316 stainless steel
Heat-passivated for a more inert surface
Stainless Steel Frit Specifications

Material:
316 Stainless Steel
Max. Temperature:
500C
Max. Pressure:
10,000psig
Typical Use:
Column Inlet/Outlet
Frit Diameter
Thickness
Porosity
5/pk
Part No.
1/4" (6.350mm)
0.031" (0.79mm)
0.031" (0.79mm)
0.063" (1.59mm)
0.031" (0.79mm)
0.031" (0.79mm)
0.063" (1.59mm)
0.063" (1.59mm)
0.031" (0.79mm)
0.031" (0.79mm)
0.031" (0.79mm)
0.063" (1.59mm)
0.5m
2.0m
2.0m
0.5m
2.0m
0.5m
2.0m
0.5m
2.0m
2.0m
2.0m
720805
721005
716505
721205
721405
718005
718205
721605
721805
722205
718605
3/8" (9.525mm)
1/2" (12.7mm)
1" (25.4mm)
25/pk
Part No.
720825
721025
716525
721225
721425
718025
718225
721625
721825
722225
718625
best
value
Stainless Steel Frits
Encased Stainless Steel Frits

Improved sealing over conventional frits
Better mechanical stability
Encased Stainless Steel Frit Specifications

Material:
316 Stainless Steel, PEEK, or Graph-Tite
Max. Temperature:
100C
Max. Pressure:
10,000psig
Typical Use:
Column Inlet/Outlet
Encased Stainless Steel Frits

Frit Diameter
Ring Diameter
Stainless Steel Frits with PEEK Ring

0.083" (2.1mm)
0.250" (6.4mm)
0.125" (3.2mm)
0.250" (6.4mm)
0.156" (4.0mm)
0.250" (6.4mm)
0.273" (7.0mm)
0.375" (9.5mm)
0.352" (10mm)
0.500" (12.7mm)
Stainless Steel Frits with Carbon-Reinforced PEEK Ring
0.083" (2.1mm)
0.250" (6.4mm)
0.125" (3.2mm)
0.250" (6.4mm)
0.181" (4.6mm)
0.250" (6.4mm)
Thickness
0.5 Micron
(5/pk)
Part No.
2 Micron
(5/pk)
Part No.
2 Micron
(10/pk)
Part No.
2 Micron
(20/pk)
Part No.
0.063" (1.59mm)
0.063" (1.59mm)
0.063" (1.59mm)
0.063" (1.59mm)
0.063" (1.59mm)
9900
9902
9906
9910
9912
99001
99021
99061
99101
99121
0.031" (0.79mm)
0.031" (0.79mm)
0.031" (0.79mm)
75015
75013
75006
75014
75012
75005
Encased Metal-Free PEEK Frits

Metal-free inert construction
Ideal for chromatography biocompatibility applications
Encased Metal-Free PEEK Frit Specifications

Material:
PEEK
Max. Temperature:
100C
Max. Pressure:
10,000psig
Typical Use:
Column Inlet/Outlet
Encased Metal-Free PEEK Frits

Frit Diameter
PEEK Frits with PEEK Ring

0.125" (3.2mm)
0.181" (4.6mm)
0.312" (7.9mm)
Ring Diameter
Thickness
5 Micron
(5/pk)
Part No.
10 Micron
(5/pk)
Part No.
0.250" (6.4mm)
0.250" (6.4mm)
0.375" (9.5mm)
0.063" (1.59mm)
0.063" (1.59mm)
0.063" (1.59mm)
69025
68152
67152
69026
69027
69028
109
analytical hplc
Empty Low-Pressure Hardware
Omnifit Low-Pressure Column Hardware
Polypropylene
Outer Cap
Borosilicate heavy wall glass with precision threaded

ends and patented strain free endfittings
Bore diameter constant to 0.1mm
Higher pressure limits than other low-pressure column
hardware
Each Omnifit glass column includes two PTFE end pieces
(one fixed, one adjustable), two 1/4-28 polypropylene outer
caps, one frit kit (two each: 10m polyethylene, 10m PTFE
and 25m PTFE), two flangeless tube endings, two gripper
fittings, and two meters of 1/16" PTFE tubing.
Adjustable
End Piece
O-ring Glass Column
Frit
Polypropylene
O-ring Outer Cap
Frit
Fixed End
Piece
2719
Omnifit Column Hardware Specifications

Material:
Borosilicate Glass, PTFE, Fluoroelastomer
Polymer, Polypropylene, and Polyethylene
Max. Temperature:
100C
Max. Pressure:
Varies, See Price Block
Thread Type:
1/4-28 UNF
Typical Use:
Low-pressure, Metal-free Columns
5551
Omnifit Glass Columns

Length
6.6mm i.d. Column, rated to 60Atm (900psig)

100mm
150mm
250mm
400mm
10mm i.d. Column, rated to 40Atm (600psig)
100mm
150mm
250mm
500mm
1000mm
100mm
150mm
250mm
500mm
1000mm
150mm
250mm
500mm
1000mm
related products
Looking for low-pressure accessories?

Low-pressure fittings, pages 117120;
Low-pressure tubing, page 387.
110
Omnifit Glass Column Accessories

Part No.
62271
62272
62273
62276
61110
61120
61140
61160
61180
64110
64120
64140
64160
64180
62121
62141
62161
62181
Description
6.6mm i.d. Column Accessories

Adjustable Plunger
25m Polyethylene Frits
25m PTFE Frits
10m PTFE Frits
5m PTFE Frits
Fluoroelastomer Polymer O-rings
Silicone O-rings
10mm i.d. Column Accessories
Adjustable Plunger
25m PTFE Frits
10m PTFE Frits
5m PTFE Frits
Silicone O-rings
Adjustable Plunger
25m PTFE Frits
10m PTFE Frits
5m PTFE Frits
Silicone O-rings
Adjustable Plunger
25m PTFE Frits
10m PTFE Frits
5m PTFE Frits
Silicone O-rings
Qty.
Part No.
ea
20
20
20
20
10
10
62287
62281
62282
62283
62284
62285
62286
ea
20
20
20
20
10
10
61651
61411
61421
61461
61471
61661
61671
ea
20
20
20
20
10
10
64651
64411
64421
64461
64471
64661
64671
ea
20
20
20
20
10
10
62651
62411
62421
62461
62471
62661
62671

HPLC Columns

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HPLC Columns

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analytical hplc

Analytical HPLC Column

Column Families Suitable for Small Molecules

hplc columns | introduction

Grace Key Column Families

VisionHT Ultra High-Pressure Columns

Alltech Alltima HP Columns

For information about Grace analytical hardware formats,

For information about prep columns, see pages 148 171.

Alltech Prevail Columns

Analytical HPLC Column Introduction

Alltech Platinum Columns

Alternate retention and selectivity for

Next generation Vydac media from the leader

Alltech Platinum phases have a controlled polar silica

Vydac MS columns provide unique selectivity and

Differentiated Phases: C18, EPS C18

Differentiated Phases: C18, C8, C4

Specifications: Spherical silica, monomerically bonded,

Specifications: Spherical silica, polymerically and

Formats: Microbore, Expedite, Rocket, SolventReducer, Analytical, Prep

Formats: Capillary, Microbore, Expedite, Rocket,

Vydac ProZap Columns

Classic reversed-phase and premium

High-speed, high-efficiency columns

GraceSmart HPLC phases use high purity silica.

Short 10 and 20mm ProZap columns with 1.5m

Differentiated Phases: C18

Specifications: Sub 2m spherical silica, proprietary

Specifications: Spherical silica, monomerically bonded,

hplc columns | introduction

Column Families Suitable for Small Molecules

Differentiated Phases: C18

Vydac Everest Columns

HPLC Column Selection

hplc columns | introduction

A Comparison of Reversed-Phase Columns

Directions for Using the Column Chart

JSphere H80 C18

Ultracarb ODS (30)

YMC Pack Pro C18 RS

Hypersil BetamaxNeutral C18

Zorbax Extend C18

Alltima HP C18 High Load

Zorbax Eclipse XDB-C18

Nucleodur C18 Gravity

Grom Sapphire 110 C18

Restek Ultra C18

Denali 120 C18

Hypersil 100 C18

Grom Sil 120 ODS-5 ST

Acclaim 120 C18

Zorbax Eclipse Plus C18

Superspher 100 RP-18e

CAPCELL C18 AG120

Grom Sil 120 ODS-3 CP

Delta-Pak C18 100A

Prodigy ODS (3)

Synergi Hydro-RP C18

YMC Pro C18