Syllabus For Bachelor of Science (Biotechnology, Chemistry, Botany) Academic Year (2012)

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Department of

LIFE-SCIENCES

Syllabus for
Bachelor of Science (Biotechnology,
Chemistry, Botany)
Academic Year (2012)

1 Semester - 2012 - Batch


Hours
Paper
Paper Per Credits Marks
Code
Week
AEN131 ADDITIONAL ENGLISH 3 4 100
BOT131 MICROBIOLOGY AND PHYCOLOGY 4 3 100
BTY131 CYTOLOGY AND GENETICS 4 3 100
CHE131 CHEMISTRY - I BASIC CHEMISTRY 4 3 100
ENG131 ENGLISH 3 3 100
FRN131 FRENCH 4 3 100
TML131 TAMIL 4 3 100
2 Semester - 2012 - Batch
Hours
Paper
Paper Per Credits Marks
Code
Week
AEN231 ADDITIONAL ENGLISH 3 4 100
BOT231 MYCOLOGY, PLANT PATHOLOGY, LICHENS 4 3 100
& BRYOPHYTES
BTY231 MICROBIOLOGY AND BIOSTATISTICS 4 3 100
CHEMISTRY - II, INORGANIC &
CHE231 4 3 100
ENVIRONMENTAL CHEMISTRY
ENG231 ENGLISH 3 3 100
FRN231 FRENCH 4 3 100
TML231 TAMIL 4 3 100
3 Semester - 2011 - Batch
Hours
Paper
Paper Per Credits Marks
Code
Week
BTY331 MOLECULAR BIOLOGY 4 3 100
CHEMISTRY - III ANALYTICAL AND
CHE331 4 3 100
INDUSTRIAL CHEMISTRY
4 Semester - 2011 - Batch
Hours
Paper
Paper Per Credits Marks
Code
Week
BTY431 GENETIC ENGINEERING 4 3 100
CHEMISTRY - IV ORGANIC AND PHYSICAL
CHE431 4 3 100
CHEMISTRY
5 Semester - 2010 - Batch
Hours
Paper
Paper Per Credits Marks
Code
Week
BTY531 IMMUNOLOGY 3 2 100
ANIMAL CELL CULTURE AND PLANT
BTY532 3 2 100
TISSUE CULTURE
CHE532 CHEMISTRY - VI PHYSICAL CHEMISTRY 3 2 100
6 Semester - 2010 - Batch
Hours
Paper
Paper Per Credits Marks
Code
Week
BIOPROCESS ENGINEERING AND
BTY631 3 2 100
ENVIRONMENTAL BIOTECHNOLOGY
ANIMAL BIOTECHNOLOGY AND PLANT
BTY632 3 2 100
BIOTECHNOLOGY
BTY652 INTERNAL PROJECT 2 1 50

Assesment Pattern

Course Marks

Theory 25% +10% +10% +5% + 50%


CIA1 (MSE) +CIA2+CIA3+Attendance + ESE

20+20+10 + 50 %
Practical
MSE +Performance+Record + ESE

CIA : Continuous internal Assessment


MSE : Mid Semester Examination

ESE : End Semester Examination

Examination And Assesments

Course Marks

25% +10% +10% +5% + 50%


Theory
CIA1 (MSE) +CIA2+CIA3+Attendance + ESE

20+20+10 + 50 %
Practical
MSE +Performance+Record + ESE

CIA : Continuous internal Assessment


MSE : Mid Semester Examination

ESE : End Semester Examination


Department Overview:
The Department of Biotechnology was started in 2007 to cater to the
increasing demand of the Biotechnology industry in India. The department
has a well-equipped laboratory for the undergraduate students. The teaching
faculties are well qualified for academics and are involved in research
projects and publications. Mandatory external summer projects and end
semester internal projects help the students get a research bend of mind.
There is a well equipped tissue culture laboratory for the students to do their
projects. Periodic visits to prominent research institutes expose the students
to the latest innovations in biotechnology. Annual seminars, workshops and
guest lectures gives ample opportunities for students to interact with the
best minds in the field, which in turn help them go for higher studies in
reputed institutes in India and abroad.

Mission Statement:
The department of Biotechnology is a platform for the overall development
of the students to gain knowledge and to develop technical skills to bloom
in academics and research to serve the mankind without disturbing the
equilibrium of the environment.

Introduction to Program:
The Bachelor of Science programme with Biotechnology, Chemistry, and
Zoology/Botany as the combinations at Christ University aim at developing
young talent for the biotechnology industry and build in them other
strengths such that they are able to venture into allied fields too. The
interactive method of teaching at Christ University is to bring about
attitudinal changes to future professionals of the industry. The
infrastructural capabilities of the department can support class strength of
80 students. Equal importance is given both to practical and theoretical
methods of learning. Apart from the syllabus, the University emphasizes on
Value Addition Programs like Current Affairs, Holistic Education,
Certificate Courses, soft skill development and entrepreneurship
programmes.

Program Objective:
The aim of this programme is to provide ?a cut above the rest? man-power
to the ever growing demands of the growing Biotechnology industry in the
country and abroad. Subjects like Cytology, Microbiology and Genetic
Engineering make the students equipped to work in laboratories, hospitals
and clinical research. Plant tissue culture and Bioprocess Engineering gives
them employable skills in tissue culture units and in Biotech industries
engaged in manufacture of drugs and vaccines.

AEN131 - ADDITIONAL ENGLISH (2012 Batch)


Total Teaching Hours for Semester:45 No of Lecture Hours/Week:3
Max Marks:100 Credits:4
Course Objectives/Course Description
Course Objectives

The Course is taught for students from different streams, namely, BA,
BSc,,BCom and BBM in the first year and for BA, BSc and BCom in the
second year. The course objectives of one syllabus for all the streams are:

to introduce the students to look at SELF through Literature


to inculcate literary sensibility/taste among students across curriculum
to improve language skills – reading, writing and listening
to equip the students with tools for developing lateral thinking

Learning Outcome
The first semester syllabus deals with the self in different situations/
predicaments, leading to self-realization and self-exploration. The pieces
chosen evolve around the self in a conflict situation, the individual as an
escapist, dealing with one's own conscience, role of relationships, and also
take up themes like drug abuse, citizen roles, fighting gender and caste bias
Unit-1 Teaching Hours:10
One Act Play
Hour of Truth - Percival Wilde
Unit-2 Teaching Hours:10
Short Story
Father and Son – Thakazhi Sivasankaran Pillai
The Duel – Maupassant
Ponnuthayi – Bama

Unit-3 Teaching Hours:15


Poetry
Snake – D H Lawrence
Story of Lost Friends – Ruskin Bond
Crutches – Bertolt Brecht
The Mouse whose name is Time – Robert Francis

Unit-4 Teaching Hours:10


Essays
Of Anger – Bacon
The Extraordinary Dinner that changed my life – The Speaking
Tree, Times of India
An essay on Drug abuse along with a case study

Text Books And Reference Books:


Essential Reading / Recommended Reading
Evaluation Pattern
BOT131 - MICROBIOLOGY AND PHYCOLOGY (2012
Batch)

Total Teaching Hours for Semester:60 No of Lecture Hours/Week:4


Max Marks:100 Credits:3
Course Objectives/Course Description
1. To understand the diversity of microbes and algae

2. To identity the diseases caused by the microbes and its control methods.

3. To enable the students to identify different species among algae


Learning Outcome
Students will be able to understand and appreciate the diversity existing
among the lower life forms. They will acquire the practical knowledge of
controlling the plant disease which may be helpful in agricultrue.
Unit-1 Teaching Hours:10
Introduction to Microbiology
Introduction to microbiology-History of Microbiology- A General account of
Microbes. Microbes from soil - Bacteria and fungi.

Microbes of Air – Bacteria and fungi.

Microbes of water – Algae fungi and bacteria.

Cyanobacteria – general charecters – Structure and reproduction of Anabaena.

Structure and reproduction of scytonema.

Structure and reproduction of Spirulina.

Economic importance of Cyanobacteria.


Unit-2 Teaching Hours:10
Viruses
Viruses:

Historical account of Viruses. Discovery of viruses

General chacters of Viruses. Classification of Viruses.

Contribution of famous scientists to Virology: Adolf Mayer, Iwanosky, Beijernik,


W.M.Stanley and Twort.

Common plant diseases: Little leaf disease of tomato or vinca rosea, yellow
mosaic of beans and papaya leaf curl.

A brief account on viroids, prions and


interferons.

Mycoplasma: General charecters and classification – structure of mycoplasmas -

A brief account of sandal-spike disease

Unit-3 Teaching Hours:12


Bacteria:
Bacteria:

General charecters of Baceria.

Classification of bacteria.

Bacterial cell wall -structure and chemistry- (gram +ve and gram -ve).

Bacterial Nutrition – autotrophic, chemotrophic, parasitic and saprophytic.

Reproduction in bacteria - asexual (budding and fission) and sexual.

A brief account of plasmids, transposons and drug resistance.


Contributions of famous scientists to Bacteriology - Leeuwenhoek, Louis Pasteur,
Griffith, Lederberg and Tatum.

Ultra structure of bacterial cell.

Economic importance of Bacteria – useful and harmful aspects – bacteria in


medicine,

food, industry, Agriculture etc.

Study of common bacterial diseases: Xanthomonas citri and agrobacterium


tumifacience. (Citrus canker and crown gall)
Unit-4 Teaching Hours:4
Phycology
Phycology:

General charecters of Algae – occurance and distribution – thallus organisation.

Reproduction types – vegetative, asexual and sexual(isogamy, anisogamy and


oogamy).

Classification of algae – fritz's classification with special reference to thallus


structure and pigmentation.

Economic importance of Algae – with special reference to - algae as food,


medicine

and industry.
Unit-5 Teaching Hours:24
Type study of Algae:
Type study of Algae:

1. Chlamydomonas – occurance and distribution, thallus morphology, reproduction


-

vegetative and sexual. Lifecycle.

2. Volvox - occurance and distribution, thallus morphology, reproduction -

vegetative and sexual. Lifecycle.

3. Hydrodictyon - occurance and distribution, thallus morphology, reproduction -

vegetative and sexual. Lifecycle.

4. Chara - occurance and distribution, thallus morphology, reproduction -

vegetative and sexual. Lifecycle.

5. Ectocarpus - occurance and distribution, thallus morphology, reproduction -

vegetative and sexual. Lifecycle.


6. Polysiphonia - occurance and distribution, thallus morphology, reproduction -

vegetative and sexual. Lifecycle.


Text Books And Reference Books:

1. Chopra, G.L. A text book of Algae, Rastogi Publications. (2005)

2. Desikachar T.V.Cyanophyta, ICAR, New Delhi. (2001)

3. Singh, Pande and Jain. A text book of botany, Rastogi Publications. (1998)

4. P.D.Sharma- Microbiology and plant pathology.(1997)

5. R.S. Malhotra- Plant Pathology. Revised edition 2004.


Essential Reading / Recommended Reading

1. Basu, A.N. Essentials of plant viruses, vectors and plant diseases, New Age
International, New Delhi. (2004).

2. Fritz R.E. Structure and reproduction of Algae, Cambridge University Press.


(2005)

3. Gunashekaran P. Laboratory manual of microbiology, New Age International,


New Delhi. (1995)

4. Salle Fundamental principles of Biotechnology, Tata McGraw Hill, New Delhi.


(1992)

5. Pelczar Microbiology, Tata McGraw Hill, New Delhi. (1999)

6. Rangaswamy G. Diseases of crop plants in India, Prentice Hall of India, New


Delhi. (1988)

7. Smith G.M. Cryptogamic Botany Vol 1, Himalaya Publications, Mumbai.


(1997)
Evaluation Pattern
BTY131 - CYTOLOGY AND GENETICS (2012 Batch)
Total Teaching Hours for Semester:60 No of Lecture Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description
A thorough knowledge about the cell components, their functions and
biochemical pathways is a prerequisite to the study of molecular biology. In this
paper the students are made to learn the structures and purposes of basic
components of prokaryotic and eukaryotic cells. Students get an idea about how
these cellular components perceive the environmental signal and respond to them
in the language of signal transduction. They also learn the mechanism of mitotic
and meiotic cell division and the relevance of accurate transfer of genetic material
during cell division. The chapters on genetics make them appreciate the flow of
inherited characters from one generation to the other.
Learning Outcome

The students can apply their knowledge of cell biology and genetics to
selected examples of changes or losses in cell function as exemplified in many
diseases. This is very much applicable these days when there is a plethora of
information on cellular mechanisms and their role in causing disease conditions
like Cancer.

UNIT 1 Teaching Hours:16


CYTOLOGY Cell and its internal Organization

Cell as a basic unit of life, Prokaryotic and eukaryotic cell, Cell theory, Cell
Organelles: Endplasmic reticulum, Golgi complex, Mitochondria,
Chloroplast, Ribosomes, Lysosomes, Peroxisomes, Nucleus (Nuclear
envelope with nuclear pore complex, nucleolus, nucleoplasm,

Chromosome - Discovery, morphology and structural Organization -


centromere, secondary construction, telomere, chromonema, euchromatin
and heterochromatin, chemical composition. Nucleosome model of
chromosome, Special types of chromosomes; Salivary gland and
Lampbrush chromosome). Cytoskeletal structures (Microtubles,
Microfilaments and Intermediate filaments) Extracellular matrix.
UNIT 2 Teaching Hours:8
Plasma membrane and its functions

Structure of Plasma membrane, Passive & Active transport, permeases,


sodium potassium pump, Ca2+ ATPase pumps, lysosomal and vacuolar
membrane ATP dependent proton pumps; uniport, symport, antiport,
modifications of membrane(Microvilli ,Gap junction, Plasmodesmata, Tight
junctions, Desmosomes) transport in prokaryotic cells; Endocytosis and
exocytosis; Receptor-mediated endocytosis; Autocrine, paracrine and
endocrine models of action.
UNIT 3 Teaching Hours:8
Ion Channels and Signal Transduction
Types of Ion-channels; Ligand-gated and Voltage-gated ion channels; Ion
channel defects; Actin, myosin, excitation - contraction coupling,
relaxation; Cytosolic, nuclear and membrane bound receptors, examples of
receptors. Second messengers - cAMP, Inositol phosphates, DAG, cGMP, G
proteins, Ca; Protein kinases, serine – threonine kinases, TNF receptor
families
UNIT 4 Teaching Hours:8
Cell Division and chromosomal variations

Types of cell division (mitosis & meiosis); details of cell cycle, cancer,
programmed cell death, Karyotype in man, A General account of structural
and numerical aberrations. Inherited disorders - allosomal (Klinefelter
syndrome and Turner’s syndrome), Autosomal (Down syndrome and Cri-
Du-Chat syndrome) Chromosomal evolution of wheat.
UNIT 5 Teaching Hours:5
Mendelism and Cytoplasmic Inheritance

Mendel’s work, laws of heredity, Test cross, Incomplete dominance and


simple problems, Plastid inheritance in Mirabilis jalapa, petite characters in
yeast and kappa particles in Paramecium.
UNIT 6 Teaching Hours:6
Interaction of Genes

Supplementary factors - Comb pattern in fowls, Complementary genes -


Flower colour in sweet peas, Multiple factors – Skin colour in human
beings, Epistasis: Plumage colour in poultry, Multiple allelism: Blood
groups in human beings.
UNIT 7 Teaching Hours:6
Sex Determination in animals, Linkage and
Crossing Over:

Concepts of allosomes and autosomes, XX-XY, XX-XO,ZW-ZZ, ZO-ZZ


types Coupling and repulsion hypothesis, Linkage in maize and Drosophila,
Mechanism of crossing over and its importance, Chromosome mapping –
Linkage map in maize.
UNIT 8 Teaching Hours:3
Mutations
De inition, causes of mutations, types of mutation: spontaneous and induced,
Mutagens: Physical and Chemical mutagens.
Text Books And Reference Books:
1. Verma P. S. and Agarwal V. K., Cell Biology, Genetics, Molecular
Biology, Evolution and Ecology: New Delhi: S. Chand and Co. Pvt.
Ltd, 2010.
2. Gardner E. J., Simmons M. J. and Snustad D. P., Principles of
Genetics: 8thEdn, USA: Wiley and sons Inc, 2006.
Essential Reading / Recommended Reading
1. Alberts Bruce, Alexander. J and Julian. L, Molecular Biology of the Cell:
5thEdn. New York: Garland Science, 2008. Print.

2. Karp Gerald, Cell and Molecular Biology: Concepts and Experiments:


6thEdn. USA: Wiley and Sons, 2009.Print.

3. Cooper G.M. and RobertH. E., The Cell: A Molecular Approach:


5thEdn. Sinauer AssociatesInc, 2009.

Evaluation Pattern
CHE131 - CHEMISTRY - I BASIC CHEMISTRY (2012
Batch)

No of Lecture
Total Teaching Hours for Semester:60
Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description

In this introductory course the students acquire knowledge of the basic


concepts of inorganic chemistry, organic chemistry, physical chemistry
and environmental chemistry. Environmental chemistry topic can
incorporate different ideas in the young minds of the students, the
importance of preserving environment in its natural form. It also
provides an insight into the harmful impacts of human intervention on
nature. Overall it emphasizes on environmental concern and a healthy
lifestyle.

Learning Outcome

Learning outcome: The fundamental concepts from all branches of


Chemistry are learnt. This learning enables the students to gain conceptual
knowledge in the relevant topics.

Unit-1 Teaching Hours:4


Periodicity of properties in elements
Definition and periodicity of the following properties - atomic radii, ionic
radii, ionisation potential, electron affinity and electronegativity
(determination not needed), lanthanide contraction, inert - pair effect with
examples.
Unit-2 Teaching Hours:4
S Block Elements

Comparative study, diagonal relationships, salient features of hydrides,


solvation and complexation tendencies including their function in
biosystems.

Unit-3 Teaching Hours:5


P Block elements

Comparative study (group-wise) of group 13 & 14 elements with respect to


periodic properties. Allotropes of carbon-Graphite and diamond. Borazines,
classification and structural principles of silicates. Structure and uses of
hydrazine, hydroxylamine, Allotropes of phosphorous.

Unit-4 Teaching Hours:4


Types of Solvents

Protic and aprotic solvents, aqueous and non aqueous solvents, liquid
ammonia as an example of non-aqueous solvent.

Unit-5 Teaching Hours:3


Classification and nomenclature of organic
compounds

Introduction, classification, IUPAC nomenclature of mono and bifunctional


organic compounds.

Unit-6 Teaching Hours:7


Structural theory in Organic Chemistry
Hybridizations, bond lengths and bond angles, bond energy; Localized and
delocalized chemical bond, polarity of bonds, resonance, hyperconjugation, inductive
and induced field effects, hydrogen bonding.Heterolytic and homolytic cleavage,
nucleophiles, electrophiles and types of organic reactions -substitution, addition and
elimination reactions; Reactive intermediates- carbocations, carbanions, free radicals
and carbenes. energy profile diagrams-transition states.
Unit-7 Teaching Hours:10
Acyclic Hydrocarbons

Alkanes

Methods of preparation with special reference to Wurtz, Kolbe and Corey


House reactions. Mechanism of free radical halogenations of alkanes.
Reactivity and selectivity of chlorination and bromination. Newman
projection and Saw horse formulae, Fischer and Flying wedge formulae.
Conformations, conformational analysis of ethane and n-butane.

Alkenes

Methods of preparation, mechanisms of dehydration of alcohols and


dehydrohalogenation of alkyl halides, regio-selectivity in alcohol
dehydration; the Saytzeff rule, Hoffmann elimination, Markownikoff’s rule,
peroxide effect, hydroboration-oxidation oxymercuration reduction,
epoxidation, ozonolysis, hydration, Birch reduction. hydroxylation and
oxidation with KMnO4, polymerization of alkenes, substitution at the allylic
and vinylic positions of alkenes; industrial applications of ethylene and
propene.

Dienes: Conjugated and isolated dienes, resonance stabilization, 1,2-versus


1,4-addition, Diels-Alder reaction.

Alkynes:Preparation, Reduction, electrophilic addition, oxidation with KMnO4,


hydroboration- oxidation and hydrogenation reactions -acidity and metal acetylides.
Unit-8 Teaching Hours:3
Alicyclic hydrocarbons (Cycloalkanes)
Nomenclature, methods of preparation and chemical reactions. Bayer’s strain theory
and its limitations. Ring strain in cyclopropane and cyclobutanes. Theory of stainless
rings systems- Sachse-Mohr theory. Conformations of cyclohexanes, axial and
equatorial bonds.
Unit-9 Teaching Hours:5
Phase rule

Statement and explanation of the terms with examples, one component


systems – water and sulphur systems. Two component systems – KI-water
and Pb-Ag systems. Eutectic and freezing mixtures and their applications.
Efflorescence and deliquescence. Phase diagram of Fe-C system.

Unit-10 Teaching Hours:5


Elementary Thermodynamics ? First law

Thermodynamic equilibrium, reversible and irreversible processes. State


and path dependent functions; exact and inexact differentials. Concept of
internal energy, heat and work. First law of thermodynamics, significance of
internal energy and enthalpy. Work done in an isothermal and adiabatic
expansion and compression of an ideal gas.

Heat capacity of a gas at constant pressure and at constant volume. relation


between the two. Kirchoff’s equation.

Unit-11 Teaching Hours:6


Colligative properties

Raoult’s law, lowering of vapour pressure. Determination of the molecular weight of


a non volatile solute using (1) Relative lowering of vapour pressure (2) Elevation in
boiling point, (3) Depression in freezing point (4) Osmotic pressure ; Osmotic
pressure of dilute solution and its measurement by Berkeley – Hartley’s method.
Osmotic laws and analogy with gas laws. Relationship between DTb, DTf and relative
lowering of vapour pressure, Abnormal molecular weight, vant Hoff factor,
evaluation of degree of dissociation and association, Rast method. Activity and
activity coefficient. Reverse osmosis and its applications.
Unit-12 Teaching Hours:4
Gases
Maxwell Boltzmann distribution of molecular velocities, mean free paths,
collision frequency, derivation of expression for most probable velocity,
definitions and expressions for rms velocity and average velocity (no
derivations), relationship between the three types of velocities.
Text Books And Reference Books:

[1]. B.R Puri., L.R Sharma and K. C Kalia. 31st edition Advanced Inorganic
Chemistry. Delhi: Shoban Lal Nagin Chand and Sons, 2011.

[2]. J. D Lee. A New Concise Inorganic Chemistry. 5th ed. London:


Chapman & Hall, 2008.

[3]. Finar I. L. A Text Book of Organic Chemistry Vol I&II. 6th ed. LBS &
Longman group Ltd. Reprinted in 1996.
Essential Reading / Recommended Reading

[1]. Bahl B. S. and Bahl A. Advanced Organic Chemistry.6th ed. S. Chand


& Co, 2006.

[2]. Daniels F and Alberty F.A. Physical Chemistry. 4th ed. Wiley, 1992.

[3]. Glasstone Samuel. Textbook of Physical Chemistry. 2nd ed. Mcmillan,


1998.

[4]. Chang Raymond .Chemistry. 6 th ed.1998.


Evaluation Pattern
ENG131 - ENGLISH (2012 Batch)
No of Lecture
Total Teaching Hours for Semester:45
Hours/Week:3
Max Marks:100 Credits:3
Course Objectives/Course Description
Description (Literature)
The pieces of prose, poetry, plays and novels chosen for the four
semesters for English have been hand picked in order to nurture and

develop fine tuned human beings for the future.

Although there is no one theme around which the texts have been

organized, it covers a wide range of ideas from history to economics to

social responsibility. Humor in some of the texts like ‘University Days’


is not simply representative of humor in the texts but also enable to

sensitize readers to what lies beneath the humor. A large

representation of Indian writers like Shashi Deshpande, R K Narayan,


Sam Pitroda, Vaikom Mohammed Basheer are included to show the very

many “Indias of the mind”. Writers from South Africa, Chile, Canada,

America, England and Sri Lanka make up the oeuvre of this selection of

texts. These have been consciously picked to help students realize the
needs that these texts talk about within our immediate context: regional,

national and global. Moreover, the textbook aims at an eclectic approach

that will lead students to appreciate, reflect, and respond to the


immediate human needs as responsible members of the younger

generation.

The selection of texts also progress in complexity with each semester,

enabling the students to gradually progress into more serious and

sustained patterns of reading and become increasingly perceptive and


conscious of their own selves and the world they see around them. In a

nutshell we aim to bring out a text that will empower the holistic

development of the generation next.

Description (Grammar and Communication Skills)

Towards the end of each selection, there will be elaborate exercises

based on the text, ranging from strengthening vocabulary to reading

comprehension to writing paragraphs and essays. Once again, with each


semester, the grammar and communication skills components will

progressively become complex and lead to more skill based, practical

exercises which are likely to help them in their careers, for example,
designing resume and writing cover letter / email correspondence.

Emphasis will be largely on reading and writing exercises. Inclusion of

the visual text and supplementary material will contribute towards


developing their listening skills. Due to lack of time and large number of

students, the aspect of speaking skills can be included by teachers

through their teaching pedagogy, for example, by making students read

aloud in the class or testing them on speaking skills through CIA’s.

This syllabus is meant to cater to all the four streams- B.A., B.Sc.,
B.Com and B.B.M., therefore the selection of texts and grammar, has

done keeping in mind the needs of all the streams. For instance students

of all the streams can relate to / understand the ideas of communal

violence and war, of development and technology. Topics of universal


concern and relevance have been included to sustain the interests of all

streams. Grammar and communication skills sections are meant

especially to help the commerce and management students, exercises on


resume making and presentation skills are likely to be of immense

relevance.

In addition to the two textbooks (Mindscapes I and II), students will be

provided with two supplementary text books for the two years. In the

first year, emphasis will be laid on developing vocabulary, grammar,


writing and communication skills. Book I will therefore include extra

exercises for the above mentioned components. In the second year,

communication skills (skill based) have been given more importance.


Hence, Book II will include components like presentation skills,

interviews, citing sources, etc. In addition to this, two poems and two
stories will also be included (since it was felt that the syllabus in

Mindscapes II is short, and more textual pieces can be accommodated in

the given teaching time).

These two books are meant to cater to students from all streams,

supplementing their learning from Mindscapes I and II, helping them to


develop certain essential, basic skills required in today’s times.
Learning Outcome
Overall Objectives

There are two main objectives of this paper.

1. To initiate the students into the realm of literature written in English


(prose, poetry, play and novel).

2. To enlighten / sensitize them to different issues / ideas ranging from

concern over carbon footprints to history to humor and different such


topics.

3. To accustom and familiarize the students with basic communication

abilities such as listening, reading, speaking, and writing, which will

help improve their overall standards of communication.

4. Objectives:

To introduce the students to texts with universal themes like war


and its effect and sensitivity towards the physically challenged

To increase their social, moral and spiritual awareness and

concern towards themselves and the society.

To sensitize them to the function of literature as elevating both-


the intellectual and emotional make up of an individual.
Basic Knowledge level:

Working knowledge of English Language, basic reading, writing,

speaking and listening skills.

Learning Outcome:

Increased knowledge of literature appreciation, vocabulary,

reading and writing skills.

Unit-1 Teaching Hours:12


Unit I: Poetry

Drummer Hodge-Thomas Hardy


Obituary- A. K. Ramanujan
Railway Station- Rabindranath Tagore

Unit-2 Teaching Hours:9


Unit II: Essay
University Days- James Thurber
On the Rule of the Road- A.G. Gardiner

Unit-3 Teaching Hours:9


Unit III: Short Stories
A Snake in the Grass- R.K. Narayan
The Eyes are not Here- Ruskin Bond

Unit-4 Teaching Hours:9


Unit IV: Play
Picnic on the Battlefield- Fernando Arrabel
Eyes are not Here- Ruskin Bond
Unit-5 Teaching Hours:6
Unit V: Visual Text
Mr. and Mrs. Iyer

Text Books And Reference Books:

Unit I: Poetry

Drummer Hodge-Thomas Hardy


Obituary- A. K. Ramanujan
Railway Station- Rabindranath Tagore

Unit II: Essay

University Days- James Thurber


On the Rule of the Road- A.G. Gardiner

Unit III: Short Stories

A Snake in the Grass- R.K. Narayan


The Eyes are not Here- Ruskin Bond

Unit IV: Play

Picnic on the Battlefield- Fernando Arrabel


Eyes are not Here- Ruskin Bond
Unit V: Visual Text

Mr. and Mrs. Iyer

Essential Reading / Recommended Reading


Evaluation Pattern
FRN131 - FRENCH (2012 Batch)
Total Teaching Hours for Semester:60 No of Lecture Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description
A propos A1 is meant for adolescents and adults and aims at the acquisition
of the competencies required in the learning of a foreign language (as per
the Common European Framework).
Learning Outcome
With its documents and numerous activities which focus on the skills -
reading, writing, speaking and communicating, A Propos 1 is an excellent
method for those wanting to prepare for the European DELF A1 exams.

Unit-1 Teaching Hours:15

1. You,me,we

2.In the family


Unit-2 Teaching Hours:15

1. What a day!

2. What would you like?


Unit-3 Teaching Hours:30

1. La chasse au lapin

2. L'avare

3. Les trocs de Jean


Text Books And Reference Books:

1. A propos A1 ( Dossier 1 -4) Langers, Saraswati House Pvt. Ltd, New


Delhi - 2010

2. Contes Dramatiques, Hills & Dondo


Essential Reading / Recommended Reading

French websites <google.fr>,< yahoo.fr>


Evaluation Pattern
TML131 - TAMIL (2012 Batch)
Total Teaching Hours for Semester:60 No of Lecture Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description
Forming part two of the undergraduate curriculum, this course on Tamil
language and literature intends to educate the youth on the rich litrerary and
cultural heritage of India, apart from sharpening the language skills
Learning Outcome
The selections from the ancient period dating back to the second century B.C.will
orient the students towards an understanding of human behavior in its varied forms
and under different circumstances
Unit-1 Teaching Hours:20
Poetry -Aham Poems 1-5
Man's relaitonship with nature is depicted along with human inter-
relationship through these poems. This is a testimony to the kind of balance
man has to maintain with nature to lead a peaceful life
Unit-2 Teaching Hours:5
Aham Poems 6-10
Aham poems deal with the complex relationship between man and woman
and how the girl's companion skilfully negotiates with the parents of the girl
to cosummate the love
Unit-3 Teaching Hours:20
Cultural Studies through Short Story
All the stories are pre-read and discussions take place in the class. The
social, political and cultural issues as seen through the sequences in the
stories are contextualized and interpretations are elicited
Unit-4 Teaching Hours:15
Grammar,Translation and Literary
Practices
Writing an errorless language is the main objective. Students are also
encouraged to have translation skills and to improve upon their critical and
analytical skills
Text Books And Reference Books:

Srinivasan, R et al (ed).Thamilppacharam I.Chennai: Kaavya


Publishers,2006

Ilakkiachinthanai. Anna Saalaiyil Or Indhiyan.Chennai: Vaanathi


Pathippagam, 2010
Essential Reading / Recommended Reading

Varadarajan, Mu. Thamil Illakkia Varalaru . New Delhi:Sahitya Akademi,


2008

Sivathambi, Ka.Thamil Sirukathaiyin Thorramum Valarchiyum.Coimbatore:


NCBH, 2009

Panjankam,Ka. Silappathikaram-Sila Payanangal.Chennai:Kaavya


Publishers, 2010

Perumalsamy, V Sangakala Samooha Nilamai, Chennai: Bharathi


Puthagalayam, 2012

Ramakrishnan S 100 Sirantha Sirukathaigal, Chennai: Discovery Books,


2013

Evaluation Pattern
AEN231 - ADDITIONAL ENGLISH (2012 Batch)
No of Lecture
Total Teaching Hours for Semester:45
Hours/Week:3
Max Marks:100 Credits:4
Course Objectives/Course Description
The Course is taught for students from different streams, namely, BA,
BSc,,BCom and BBM in the first year and for BA, BSc and BCom in the
second year. The course objectives of one syllabus for all the streams are:

to introduce the students to look at SELF through Literature


to inculcate literary sensibility/taste among students across curriculum
to improve language skills – reading, writing and listening
to equip the students with tools for developing lateral thinking

Learning Outcome
The second semester Additional English syllabus places the self in a
context; the context of a society or the nation ; hence the syllabus takes
into account our social, economic and emotional issues, forces us to have
a perceptive outlook on relationships, has some humorous pieces about
Indian English proficiency and also has a thought-provoking essay on
India's educational system. Some of the stories talk about the turbulent
times in India and the individual's role and responsibility during such
traumatic situations.
Unit-1 Teaching Hours:15
Novella
Train to Pakistan – Khushwant Singh
Unit-2 Teaching Hours:7
Short Story
Another Community – R K Narayan
Father: Prafulla Roy (Translation from Bengali by John W Hood)

Unit-3 Teaching Hours:11


Poetry
· Letter to a Dear Friend – L Kharma Wphlang

· The Mistress – Keki N Daruwalla

· Enterprise - Nissim Ezekiel


Unit-4 Teaching Hours:12
Essays
· Writing on the wall – Binoo John

· Social Responsibility – Subroto Bagchi

· India’s Educational system – Mata Smadarshini


Text Books And Reference Books:
Essential Reading / Recommended Reading
Evaluation Pattern
BOT231 - MYCOLOGY, PLANT PATHOLOGY,
LICHENS & BRYOPHYTES (2012 Batch)
Total Teaching Hours for Semester:60 No of Lecture Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description
1. to enable the students to understand diversity among fungi and
bryophytes.

2. to make them aware of the evolutionary link between different forms of


lives in plant kingdom.

3. To understand the common plant pathogens and its etiology and control
methods.
Learning Outcome
Students will be able to understand and appreciate the diversity exjstjng
among the plants. They will be trained to identify the different species of
Fungi and bryophytes.
Unit-1 Teaching Hours:5
Fungi
A general charecters of fungi. Classification of fungi – Alexopaulos.

Economic importance of fungi – beneficial and harmful aspects.

Role of fungi in agriculture, medicine and industry.


Unit-2 Teaching Hours:15
Type Study
Type study:

1. Albugo – occurance and distribution, structure of thallus, reproduction

and lifecycle.

2. Peziza - occurance and distribution, structure of thallus, reproduction

and lifecycle.

3. Puccinia - occurance and distribution, structure of thallus, reproduction

and lifecycle.

4. Cercospora - occurance and distribution, structure of thallus, reproduction

and lifecycle.
Unit-3 Teaching Hours:10
Plant Pathology
Plant pathology: History of plant pathology.

A general account of symptoms, etiology, epidemiology and control of:

a. Koleroga b. Blast disease of rice c. Red rot of sugar cane

d. Coffee rust e. Grains smut of sorghum.

Forecasting of plant diseases.


Unit-4 Teaching Hours:10
Lichens
Lichens: A general account and types.

Economic importance of Lichens.

Mycorrhiza: A general account.


Unit-5 Teaching Hours:20
Bryophytes
Bryophytes – A general account and classification of bryophytes.

Distribution, structure, reproduction and alternation of generations ofMarchantia,


Anthoceros and Funaria.
Text Books And Reference Books:

Text books

1. Alexopoulos (1992) an introduction to Mycology, New Age International,


New

Delhi.

2. P. D. Sharma. Fungi and allied organisms. Narosa publishing house pvt.ltd.

Jodhpur2005.

3. Pathak.V. Fundamentals of plant Pathology. Agrobios (India).2008.

4. R. N. Chopra and P.K. Kumar. Biology of Bryophyta. New age international.

New Delhi. 2005.

5. Brown. R. G.Plant diseases and their control. Sarup & Sons.NewDelhi.1999.


Essential Reading / Recommended Reading

5. Vashista. B.R. Bryophyta. S.Chand and Co, New Delhi. 2007

6. Vasishta B.R. Fungi S. Chand & Co.Ltd. New Delhi. 2005

7. Rajan. Introduction to Fungi, L.B. Publications.Newdelhi.2005

9. Basu A.N. Essentials of Plant Viruses, vectors and plant diseases, New Age

International, New Delhi. 1993

10. Pelczar .Microbiology, Tata McGraw Hill, New Delhi. 2006

11. Rangaswamy G. Diseases of crop plants in India, Prentice Hall of India,

New Delhi. 2007.

12. R.C.Dubey & Maheshwari. D. K. A text book of Microbiology. S. Chand


&

Co.Ltd. New Delhi. 2007.


Evaluation Pattern
BTY231 - MICROBIOLOGY AND BIOSTATISTICS
(2012 Batch)

Total Teaching Hours for Semester:60 No of Lecture Hours/Week:4


Max Marks:100 Credits:3
Course Objectives/Course Description
The paper surveys bacteria, viruses, fungi, and protozoa, as well as
their growth characteristics and morphology. This course guides the students
to learn appropriate microbiology laboratory techniques that will be useful
in subsequent courses. This course fulfills the basic knowledge in
microbiology for those students who wish to pursue career in allied health
fields and other technical programs. The students are made aware of GLP
(good laboratory practices) which is very important in laboratories.
Biostatistics portions will introduce the students to clinical research and
imparts knowledge on applied statistics.
Learning Outcome

Most of the techniques in biotechnology uses bacteria, viruses and


fungi. This course will make the students adept in the structure and
functions of these microbes which in turn will give them confidence to work
using these organisms. The students will become competent for jobs in
dairy, pharmaceutical, industrial and clinical research.

UNIT 1 Teaching Hours:11


Introductory Microbiology

Definition and History - Contributions of Antony van Leeuwenhoek, Louis


Pasteur, Robert Importance and scope of Microbiology as a modern Science
.Branches of microbiology. Microbial Taxonomy: Methods of Isolating pure
culture, Classification and nomenclature of Microorganisms.

Microscopy – Light, Phase contrast, Fluorescence & Electron: Stains and


staining techniques - Gram’s, acid fast, capsular, flagellar and endospore
staining. Methods of Sterilization; Physical and Chemical control of
microorganisms.
UNIT 2 Teaching Hours:15
Structure and Classification of
Microorganisms

Structureof bacteria –shape, flagella ,endospore and capsule, structure based on


staining reaction (Gram positive and Gram negative bacteria), extreme environment.
Viruses – Structure and classification, Plant Viruses – CaMV, Animal viruses –
Hepatitis B Herpes Simplex Virus, Bacteriophages.
Eukaryotic microorganisms- Salient features, classification and reproduction of fungi,
mycoplasma and algae


UNIT 3 Teaching Hours:10
Physiology and biochemistry of microbes

Nutrition (Photoautotrophs, Chemoautotrophs), Parasitism, Saprophytism,


Mutualism and Symbiosis, Commensalisms, endozoic microbes) -
Respiration: EMP, HMP and ED Pathways, Bacterial photosynthesis:
Photosynthetic apparatus in prokaryotes, Photophosphorylation. Nitrogen
metabolism (nitrogen fixation)
UNIT 4 Teaching Hours:4
Pathogenic Microorganisms

Epidemics, endemics and pandemics, Bacterial diseases of man – Tetanus,


Tuberculosis, Pneumonia and Cholera, Viral diseases: AIDS – Lifecycle of
HIV, Parasites: Lifecycles of Entamoeba and Malaria.
UNIT 5 Teaching Hours:3
BIOSTATISTICS Importance and
application
Tabulation and classi ication of data, Frequency distribution and Graphical
distribution of data
UNIT 6 Teaching Hours:8
Measures of central tendencies and
Dispersion
Mean, Median, Mode and their properties, Mean deviation, Variance, Standard
deviation and coef icient of Variation
UNIT 7 Teaching Hours:3
Hypothesis testing
Student T and Chi-square test
UNIT 8 Teaching Hours:6
Probability and Distribution

Concepts and problems on probability. Binomial, Poisson, Normal


Distribution and their applications
Text Books And Reference Books:

1. Pelczar M. J., Chan E. C. S. and Kreig N. L., Microbiology New


Delhi: Tata Mcgraw Hill Education Pvt Ltd., 2004

2. Dubey and Maheswari, Microbiology, New Delhi: S. Chand &


Company Ltd., 2010.

Essential Reading / Recommended Reading

1. Madigan M.T., Martinko J. M. and Parker J., BROCK’S BIOLOGY,


12thedn. Benjamin Cummings, 2008.

2. Tortora, Funke, Case. An Introduction to Microbiology, 10thedn., USA:


Pearson Education, 2008.

3. Prescott Lansing, Harley John and Klein Donald.Microbiology. 6thedn.,


Australia:. Mcgraw Hill 2004.
4. Daniel W. M. Biostatistics: A Foundation for Analysis in the Health
Sciences, 9th Edn, USA: Wiley and Sons, 2008.
Evaluation Pattern
CHE231 - CHEMISTRY - II, INORGANIC &
ENVIRONMENTAL CHEMISTRY (2012 Batch)
No of Lecture
Total Teaching Hours for Semester:60
Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description
In this course they acquire a deeper knowledge about inorganic chemistry and
learn the fundamentals of nuclear chemistry and solid state chemistry as this is
required for a better perspective of the subject. This course also instills values of
ethical and judicious use of nuclear energy. Environmental chemistry topic can
incorporate different ideas in the young minds of the students, the importance of preserving
environment in its natural form. It also provides an insight into the harmful impacts of human
intervention on nature. Overall it emphasizes on environmental concern and a healthy lifestyle.
Learning Outcome
Students understand and apply scientific concepts, principles, and theories pertaining
to the systematic arrangement of elements of the nature. Gain an insight into nuclear
chemistry.
Unit-1 Teaching Hours:7
Atomic Structure and Elementary Quantum
Mechanics

Blackbody radiation, Planck’s radiation law, photoelectric effect, Compton


effect, de Broglie’s hypothesis, Heisenberg’s uncertainty principle.
Postulates of quantum mechanics. Schrodinger wave equation and particle
in a box, energy levels, wave functions and probability densities.
Schrodinger wave equation for H-atom. radial and angular functions,
hydrogen like wave functions, quantum numbers and their importance.

Unit-2 Teaching Hours:15


Chemical Bonding

(i) Ionic Bond – Types of ionic solids, radius ratio effect and coordination
number, limitations of radius ratio, lattice defects, lattice energy and Born-
Haber cycle, solvation energy and solubility of ionic solids, polarizing
power and polarizability, Fajan's rules.
(ii) Covalent Bond : Valence bond theory and its limitations, directional
characteristics of covalent bond, various types of hybridization and shapes
of simple inorganic molecules and ions such as NH3, H3O+, SF4, ClF3

ICl2¯,ICl4-, and H2O by valence shell electron pair repulsion (VSEPR)


theory, linear combination of atomic orbitals (LCAO), bonding, nonbonding
and antibonding molecular orbitals. Applications of MO theory to explain
the stability of homo and hetero dinuclear diatomic molecules, multi-centre
bonding in electron-deficient molecules - diborane.

(iii) Metallic bond- Free electron and band theories.

iv) Weak Interactions-Hydrogen bonding- Inter molecular hydrogen


bonding and intra molecular hydrogen bonding, van der waal’s forces.

Unit-3 Teaching Hours:5


3. Physical properties and Molecular Structure
Polarisation and orientation of dipoles in an electric field. Dipole moment. Induced
dipole moment . Clausius-Mossotti equation (only statement). Dipole moment and
structures of molecules (planar and non-planar). Magnetic properties: paramagnetic,
diamagnetic and ferromagnetic systems.
Unit-4 Teaching Hours:10
Nuclear chemistry

N/P ratio, curves, stability belts. Nuclear binding energy. Mass defect,
simple calculations involving mass defect and B.E per nucleon, half-life,
radioactive equilibrium, radioactive series, magic numbers - liquid drop
model - shell model. Artificial radioactivity - Induced radioactivity, Nuclear
fission - nuclear energy - nuclear reactors - breeder reactor - nuclear fusion -
thermonuclear reactions - energy source of the sun and stars.

Isotopes—use of radioactive isotopes in tracer technique, agriculture,


medicine, food preservative and carbon and tritium dating. (Problems to be
worked out).

Unit-5 Teaching Hours:3


Noble Gases
Properties of noble gases, Xenon; properties, structure and bonding in Xenon
compounds.
Unit-6 Teaching Hours:20
Environmental Chemistry
Atmosphere: Composition and structure of atmosphere, particles, ions and
radicals in the atmosphere, stratospheric ozone depletion.

Air Pollution: Air Pollutants, e.g. carbon monoxide, nitrogen oxides,


hydrocarbons, oxides of sulfur, photochemical smog, acid rain and
particulates. Air quality standards and sampling; monitoring of CO, NOx,
and SO2. Automobile pollution. Effect of pollutants.

Hydrosphere : Water resources, hydrologic cycle. Complexation in natural


and waste water. Micro organisms.

Water Chemistry: Principles and applications of aqueous chemistry and


unique properties of water. Water quality parameters and standards. Acid
mine drainage, heavy metal pollution, eutrophication, soap and detergents,
pesticides, nitrates, fluorides- effect and abatement.

Lithosphere : Composition, inorganic & organic compounds in soil, Acid-


base and ion exchange reaction in soil, micro and macro nutrients, nitrogen
pathways and NPK in soil, waste classification & disposal. E-waste and its
disposal.

Radiation hazards and disposal of nuclear wastes.

Green chemistry: Importance, principles and scope of green chemistry.


Text Books And Reference Books:

[1].B.R Puri., L.R Sharma and K. C Kalia. 31st edition Advanced


Inorganic Chemistry. Delhi:Shoban Lal Nagin Chand and Sons, 2011.

[2].B.R Puri., L.R Sharma and Pathania. 46th edition Principles of Physical
Chemistry. Vishal Publishing Company, 2012.

[3]. Satyaprakash, Tuli, Basu and Madan. Advanced Inorganic Chemistry


Vol-I.1986.

[4]. Ajay Kumar Bhagi and Chatwal G.R.Environmental Chemistry.


Himalaya Publishing house, 2003.

[5]. Desai K.R. Green Chemistry. Himalaya publishing house, 2005.


[6]. Anil Kumar de and Arnab Kumar de. Inorganic Chemistry and analysis.
2 nd ed. New age international publication: 2005.

[7]. Arnikar H.J. Essentials of Nuclear Chemistry. 2nd ed.John Wiley and
Sons, 1987.
Essential Reading / Recommended Reading
Evaluation Pattern
ENG231 - ENGLISH (2012 Batch)
No of Lecture
Total Teaching Hours for Semester:45
Hours/Week:3
Max Marks:100 Credits:3
Course Objectives/Course Description
Objectives:

To increase the level of intellectual engagement in terms of complexity


of themes of the texts.
To engage the students’ attention towards different literary forms and
writing, for example, reading Hughes and Ezekiel requires a deeper
understanding of the form of prosody and poetic structures.
To also initiate the students to world literature through Neruda and
Gordimer.

Knowledge level:

Working knowledge of English Language, basic reading, writing,


speaking and listening skills

Learning Outcome
Learning outcome:

· Increased proficiency in reading, writing, speaking and listening skills.

Unit-1 Teaching Hours:15


Unit I: Poetry
Mending Wall- Robert Frost
Dream Deferred- Langston Hughes
The Patriot- Nissim Ezekiel
Tonight I can Write- Pablo Neruda

Unit-2 Teaching Hours:15


Unit II: Essay
Of Kitchens and Goddesses- Shashi Deshpande

Unit-3 Teaching Hours:15


Unit III: Short Stories
And of Clay are we Created- Isabel Allende
Once Upon a Time- Nadine Gordimer
Neipayasam- Kamala Das

Text Books And Reference Books:

Mindscapes 1
Essential Reading / Recommended Reading
Evaluation Pattern
FRN231 - FRENCH (2012 Batch)
Total Teaching Hours for Semester:60 No of Lecture Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description
A propos A1 is meant for adolescents and adults and aims at the acquisition
of the competencies required in the learning of a foreign language (as per
the Common European Framework).
Learning Outcome
With its documents and numerous activities which focus on the skills -
reading, writing, speaking and communicating, A Propos 1 is an excellent
method for those wanting to prepare for the European DELF A1 exams.

Unit-1 Teaching Hours:15

1. Here and there

2.Elsewhere
Unit-2 Teaching Hours:15

1. At work

2. Living together
Unit-3 Teaching Hours:30
One Act Plays
1. Le médecin malgré lui
2. L’étoffe merveilleuse

3. Le Picard et le Gascon
Text Books And Reference Books:

1. A propos A1 ( Dossier 5-8) Langers, Saraswati House Pvt. Ltd, New


Delhi - 2010

2. Contes Dramatiques, Hills & Dondo

Essential Reading / Recommended Reading

Related french websites


Evaluation Pattern
TML231 - TAMIL (2012 Batch)
Total Teaching Hours for Semester:60 No of Lecture Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description
This paper touches upon the other aspeects of the social life of Sangam age
covering their occupation, worship, customs, manners and faith. The flora
and fauna details are also observed. Selection of passages from classical
epics like Kambaramayanam will see the cultural variations that are
different from the Sanskrit epic, Valmiki Ramayanam
Learning Outcome
This paper puts Tamil literature in historical continuity. It also familiarizes
the students with the contemporary culture and the crucial issues concerning
modern life
Unit-1 Teaching Hours:20
Social Life of Sangam Age
Having introduced the Aham like of the Sangam people in the first semester
students are exposed to their social and political life. The bards act as the
conscience keepers for the state and their quest for peace during troubled
times speak of the noble value system followed during that era
Unit-2 Teaching Hours:15
The Classical Epic
'Kambaramayanam' has all the epic qualities and the chapter 'Kumbakarnan
Vathaippadalam' has deeper conflict of emotions. Kumbakarnan's dilemma
whether to choose 'dharma' or help his unjust, unethical elder brother is
resolved through long conversations with the main characters
Unit-3 Teaching Hours:15
Folk Religion and Visual Text
India has a tradition of following both vedic and non-vedic religious
practices. 'Thamizh Mannin Saamigal' makes a journey through the folk
gods and goddesses found in various parts of Tamilnadu and describes the
customs and practices of the folk tha worship them. This shows the variety
that characterizes the cultural fabric of our country.

The visual text, the Tamil Feature film, 'Aadugalam' is a perfect example for
the blend of technology and art. The 'open text' offers enough scope for
various socio-cultural interpretations by the students
Unit-4 Teaching Hours:10
Grammar,Translation and Literary
Practices
This unit takes care of the language skills that an undergraduate student is
expected to possess. This is done through quiz, newspaper readings, role
play and reviewing programmes from television and internet
Text Books And Reference Books:

Poetry: Sangam Literature-10 Puram Poems. Later Epics-


Kambaramayanam- Yuddhakandam, Kumbakarnan Vathaippadalam.

Prose: Thamizh Mannin Samigal by Mana

Visual Text: Film Aadukalam (2011)


Essential Reading / Recommended Reading

Va.Su. Pa Manickam, Va.Su.Pa. Kambar.Chennai: Manivasagar, 2005

Varadarajan, Mu. Thamil Illakkia Varalaru . New Delhi:Sahitya Akademi,


2008

Sivasubramanian, A. Mandiramum Sadangukalum. Nagercoil:


Kalachuvadu, 2010

Theodore, Baskaran, Thamil Cinema Or Arimugam. Chennai: Kilakku


Pathippagam, 2012

Pavendan, Dhiravida Cinema, Chennai: Kayal Kavin Books, 2013

Paramasivan, Tho. Vidupookkal, Chennai: Kayal Kavin Books, 2014


Evaluation Pattern
BTY331 - MOLECULAR BIOLOGY (2011 Batch)
No of Lecture
Total Teaching Hours for Semester:60
Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description

This paper aims to provide students with an indepth understanding of the basic
concepts of molecular biology. The structural and functional aspects of basic
biomolecules such as DNA, RNA and protein and the mechanisms of DNA
replication, transcription, translation and gene regulation will be dealt with. The
course facilitates the students to have a strong understanding of the molecular
basis of life and the underlying genetic principles.

Learning Outcome

The main outcome of this paper is that the student will have the basic in depth
knowledge about the macromolecules that store, transmit and execute the
genetic information in a living system and the molecular mechanism of the
information low in the living system. This theoretical knowledge on molecular
biology will help the students to learn basics of the genetic engineering and
rDNA technology work which makes the basis of modern biotechnological
research and industry.

Unit-1 Teaching Hours:3


Introduction to Molecular biology
Molecular basis of life, Experimental Proof for DNA and RNA as genetic
material.
Unit-2 Teaching Hours:8
Nucleic Acids
Structure and functions of DNA, Watson and Crick model of DNA, forms for
DNA (A,B, C, D and Z DNA), physical, chemical and spectroscopic properties of
DNA, topoisomerases and DNA supercoiling, Structure and functions of RNA,
Types of RNA – mRNA, tRNA, rRNA, snRNA. Ribozymes – signi icance.
Unit-3 Teaching Hours:8
DNA Replication

Prokaryotic DNA replication – Semiconservative DNA replication experiments,


Structure and function of DNA Polymerases and other enzymes, initiation,
elongation and termination. Eukaryotic DNA replication – Enzymes and proteins
involved in replication, initiation, elongation and termination.Telomere
replication and its signi icance. Models of DNA replication: Theta model and
Rolling circle model. Inhibitors of DNA replication

Unit-4 Teaching Hours:4


DNA Damage and Repair
DNA damage: DNA lesions, oxidative damage, alkylation, UV damage.Repair
mechanisms - Mismatch repair, photoreactivation, excision repair, SOS repair.
Unit-5 Teaching Hours:4
Recombination in Prokaryotes

Transformation – Tatum and Lederberg’s experiment, Conjugation – F+ and F-


strains, Hfr strains. Transduction -Lytic and lysogenic life cycles of
bacteriophage, generalized and specialized transduction

Unit-6 Teaching Hours:3


Structure of prokaryotic and eukaryotic
gene
Monocistronic and polycistronic genes, C value paradox, genetic code -
properties and Wobble hypothesis
Unit-7 Teaching Hours:8
Transcription in Prokaryotes and
Eukaryotes
Mechanism - initiation, elongation and termination, promoters and RNA
polymerase, transcription factors, Post transcriptional modi ications of
eukaryotic mRNA- polyA tailing, splicing, capping
Unit-8 Teaching Hours:7
Translation
Mechanism of translation in prokaryotes and eukaryotes, post translational
modi ications of proteins- glycosylation, protein folding.
Unit-9 Teaching Hours:8
Regulation of Gene expression
Regulation of Gene expression in Prokaryotes – Operon concept (lac and trp),
Regulation of Gene expression in Eukaryotes – RNA interference, DNA and
Histone modi ication, galactose metabolism in yeast.

Unit-10 Teaching Hours:3


Extra nuclear DNA
Gene organization and expression in Mitochondria and chloroplast, functions,
signi icance, role in evolutionary studies.

Unit-11 Teaching Hours:4


Insertional elements
Transposable elements- classes, transposons in bacteria, Maize and Drosophila,
Retroposons, LINES and SINES, transposons and mutations
Text Books And Reference Books:

1. Freifelder D. and Malacinski G., Essentials of Molecular Biology, 3rd


edition, Jones & Bartlett Publishers, 1998.

2. Rastogi V. B., Fundamentals of Molecular Biology, New Delhi: Ane


Books India, 2008.

Essential Reading / Recommended Reading


1. Howe C, Gene Cloning and Manipulation, USA: Cambridge
University Press, 1995.

2. Lewin B., Genes VIII, New York: Oxford University Press, 2003.

3. Lodish H., Berk A., Matsudaira P., Kaiser C. A., Krieger M., Scott M.
P., Zipursky L., and Darnell J., Molecular Cell Biology, 6th edition,
W.H. Freeman & Company, 2007.

4. Turner P., Mclennan A., White R. H., and Bates. A. D.,Instant Notes in
Molecular Biology, 3rd edition, Taylor & Francis Books India Pvt Ltd,
2005.

5. Karp G., Cell & Molecular Biology, 3rd Edition, John Wiley & Sons
Inc, 2002

6. Gardner E. J., Simmons M. J. and Snustad D. P., Principles of


Genetics, 8th edition,Delhi: Wiley India Ltd, 2006.

Paul A. Text book of Cell and Molecular Biology, 2nd edition, Kolkata: Books and
Allied Pvt Ltd, 2
Evaluation Pattern
CHE331 - CHEMISTRY - III ANALYTICAL AND
INDUSTRIAL CHEMISTRY (2011 Batch)
No of Lecture
Total Teaching Hours for Semester:60
Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description
This course deals with the additional concepts of Inorganic and Physical
Chemistry and lays the foundation for more advanced topics in the subsequent
courses. It emphasizes on the judicious use of energy resources.
Learning Outcome
Students gain insight into the rates of reactions and factors that affect it; coordinate
bonding and powder metallurgy
Unit-1 Teaching Hours:3
Petroleum and Petrochemicals
Origin of petroleum, composition, Octane number, Cetane number, petrol, diesel,
kerosene, naphtha, lubricants, LPG (mention only), synthetic petrol, fuel additives-
petrochemicals
Unit-2 Teaching Hours:3
Soaps and Detergents
Comparison of soaps and detergents, synthesis of detergents starting from
benzene, mechanism of action of soaps and detergents. Detergents
formulations, laundry detergent formulations, all purpose cleaning products,
special purpose cleaners.
Unit-3 Teaching Hours:2
Organic Dyes
Relation between colour and structure, modern views, synthesis of
malachite green, congo red and methyl orange. Structure and synthesis of
indigo and alizarin. Chemistry of dyeing.
Unit-4 Teaching Hours:5
Polymers
Polymers

Types of polymers and polymerisation process, Addition polymers, stereo


controlled polymers, condensation polymers, radical, ionic mechanism of
polymerisation. Preparation and applications of following polymers : (i)
Natural and synthetic rubber, (ii) Synthetic fibers : Polyester, polyamides,
polyacrylates and rayons, (iii) Plastics : Polyolefines and Polyurethanes,
(iv) Foaming agents : Plasticizers (v) Biodegradable polymers and their
advantages.
Unit-5 Teaching Hours:15
Industrial materials
i) Non-ferrous alloys: production, applications (copper and Nickel alloys)

ii) Refractories :properties , classification, determination of PCE values.

iii) Abrasives: classification, applications, hardness, manufacture and


importance of carborundum , alundum , tungsten carbide.

iv) Glass: Properties, types, manufacture of soda, borosilicate and optical


glasses. Safety glass, fire and bullet proof glasses .

v) Fertilizers: Qualities of a good fertilizer; Role of N, P, K in fertilizer.


Manufacture of Calcium Cyanamide (CaCN2) and its characteristics.

vi) Cement: raw materials, grades, manufacture of Portland cement, setting


process.

vii) Paints and varnishes: constituents of oil and emulsion paints and their
role, constituents of varnishes.

viii) Pigments : manufacture and relative merits of white lead , lithopone


and titanium white.
ix) Fuels : Characteristics , calorific value and its determination using bomb
calorimeter . Coal - varieties, reserves and production in India. Production
of coke, Gaseous fuels – advantages, constituents and their significance.
Biofuels.

x) Explosives: Classification, preparation of dynamite and TNT.

xi) Propellants: characteristics, classification and their applications


Unit-6 Teaching Hours:2
Powder Metallurgy
Advantages of powder metallurgy, applications, techniques in production of
metal powders, production of tungsten powder from Wolframite.
Unit-7 Teaching Hours:3
Statistical evaluation of analytical dat
Concept, classification and minimization of errors; Average, Mean and standard
deviation Accuracy and Precision, Significant figures in computation
Unit-8 Teaching Hours:5
Principles involved in inorganic qualitative
analysis
Common ion effect, solubility product, condition for the precipitation of a
cation in solution, grouping of radicals, selective precipitation of cations in
basic radical analysis. Complex ion formation- instability constant,
detection of Cu2+ and Cd2+ from a mixture. Interfering radicals and their
elimination.
Unit-9 Teaching Hours:3
Solvent Extraction
Nernst distribution law, Applications, Batch and continuous extraction of
solids and liquids -Soxhlet extraction of phytochemicals.
Unit-10 Teaching Hours:6
Chromatography
Classification of chromatographic methods, Column chromatography-
terminology, basic principles of adsorption, partition and ion exchange
chromatography. Principles and applications of HPLC, GC, TLC.
Unit-11 Teaching Hours:7
Instrumental methods of analysis
Spectrochemical methods- Colorimetry and Spectrophotometry, Technique
and instrumentation. Beer lambert’s law and its applications, deviations,
limitations. flame photometry- determination of sodium and potassium.

Electro analytical methods- estimation of copper by electrogravimetric


method, Thermo analytical methods-TGA, DSC.
Unit-12 Teaching Hours:2
Titrimetric methods of analysis
Acid base, Redox, Precipitation and Complexometric titrations. Theory of
Indicators and their classification – acid base, mixed and flourescent
indicators.
Unit-13 Teaching Hours:4
Organic reagents in analytical chemistry-
4Hrs
Advantages of organic reagents over inorganic reagents ; use of EDTA,
Oxine, DMG and o-Phenenthroline in the estimation of Mg, Ca, Zn, Ni (by
gravimetry and volumetry) and Fe (by colorimetry).
Text Books And Reference Books:

1. Sharma B.K.,Introduction to Industrial Chemistry. Goel Publishing


House, 1998.

2. Arora M.G.and Singh M., Polymer chemistry, Anmol publications pvt


ltd., 1996.
Essential Reading / Recommended Reading

3. Willard H. H., Merritt L. L. and Dean J. A., Instrumental Methods of


Analysis, 6th Edition, CBS Publishers & Distributors, Shahdara, Delhi,
1986.

4.ChristianG. D.,Analytical Chemistry, 4th Edition, New York: John Wiley


& Sons, 1986.

5. Bassett J., G.H. Jeffery and J.Mendham, and R.C. Denny, Vogel’s text
book of qualitative chemical analysis, 5th ed., Longman Scientific and
Technical, 1999.

6. Skoog, West, Holler and Crouch, Fundamentals of analytical chemistry,


8th edition, Thomson Asia Pvt. Ltd, 2004.

Evaluation Pattern
BTY431 - GENETIC ENGINEERING (2011 Batch)
No of Lecture
Total Teaching Hours for Semester:60
Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description
The aim of this paper is to provide an introduction to recombinant DNA
technology. It helps the students to understand how the principles of
molecular biology have been used to develop techniques in recombinant
DNA technology. The objective of the course is to familiarize the student
with the basic concepts in genetic engineering - enzymes, cloning vehicles,
gene libraries, analysis and expression of the cloned gene in host cell and
understand ethical issues and biosafety regulations. It gives emphasis to
practical applications of genetic engineering tools in the field of health care.
Learning Outcome

Modern Biotechnology relies on rDNA technology. This paper will equip


the student with all the basic rDNA methods and protocols. They will be
aware of the biosafety and ethical aspects of the same. Moreover, the
students will be able to find a job in R&D laboratories/industries where
rDNA works are being done.

Unit-1 Teaching Hours:1


Introduction to Genetic Engineering

Brief history, advantages and disadvantages of Genetically Modified


Organisms.
Unit-2 Teaching Hours:7
Tools for genetic engineering
Restriction enzymes – function, classi ication, restriction modi ication system.
DNA ligases –types and function. DNA modifying enzymes- functions. Gene
cloning vectors – Plasmids, bacteriophages, cosmids, phagemids, YACs, and
BACs.
Unit-3 Teaching Hours:4
Creation of recombinant DNA
In vitro construction of recombinant DNA molecules – (pBR 332, pUC 18)
Isolation of insert and & vector DNA, creation of r-DNA.
Unit-4 Teaching Hours:3
Transformation of r-DNA

Transformation of recombinant DNA molecules into target host Organisms -


Calcium chloride mediated, Electroporation, Biolistic gun, lipofection and
microinjection.
Unit-5 Teaching Hours:2
Screening
Immunological screening and colony and plaque hybridization, dot blot
hybridization, chromosome walking
Unit-6 Teaching Hours:2
Gene Libraries

Construction of genomic and cDNA libraries, selection of vectors.


Unit-7 Teaching Hours:3
Expression of cloned DNA
Protein production by foreign DNA in the host bacteria E. coli, cloning in
pET vectors, eukaryotic vectors- Baculovirus based vectors, mammalian
viral vectors.
Unit-8 Teaching Hours:4
Electrophoretic Techniques

Agarose gel electrophoresis, Polyacrylamide gel electrophoresis (PAGE) -


Native PAGE and SDS PAGE – gel casting and running, troubleshooting.
Unit-9 Teaching Hours:4
PCR and Site directed mutagenesis
Polymerase chain reaction (PCR) - Basic principle, methodology, types of PCR-
Inverse PCR, Hot start PCR, RT PCR, Real time PCR, RAPD, RFLP and AFLP.
Mutagenesis – principle, types, applications
Unit-10 Teaching Hours:4
Nucleic acid Sequencing
Maxam Gilbert method, Sanger’s dideoxy method, automated DNA sequencing,
applications, DNA Fingerprinting - principle, applications in forensics, paternity
testing
Unit-11 Teaching Hours:5
Blotting Techniques

Southern and Northern blot, probe preparation – radioactive and


chemiluminiscent methods, hybridization technique, labeling (Random
primer labeling, Nick Translation), washing, autoradiography. Western Blot,
hybridization, Sandwich and dot ELISA.
Unit-12 Teaching Hours:4
Application of r-DNA
Production of recombinant proteins (Insulin), recombinant vaccines (Hepatitis
B), hormones (Human growth hormone), Diagnostic kits, Gene therapy – types,
applications, ethical issues
Unit-13 Teaching Hours:2
Biosafety issues and environmental
significance

Environmental significance of genetically modified microbes, plants and


animals, release of GMOs, factors affecting biodiversity, invasiveness, and
gene flow, possible influences of transgenic crops on non-target organisms,
international treaties/agreements in biosafety, public perception and
outreach in biosafety.
Text Books And Reference Books:

1. Winnacker Ernst-Ludwig, From genes to clones: Introduction to gene


technology, Panima Publishing Corporation, 2003.

2. Paul A. Text book of Cell and Molecular Biology. 2nd edition,


Kolkata, Books and Allied Pvt Ltd, 2007.

Essential Reading / Recommended Reading


1. Glick B. R. & Pasternak J. J, Molecular Biotechnology, Principles and
Applications of Recombinant DNA, Washington D.C.: American
Society for Microbiology, 2003.

2. Brown T. A., Gene Cloning and DNA Analysis: An Introduction,


Wiley Blackwell Publishers, 2001.

3. Nicholl D. S. T., An Introduction to Genetic Engineering. Cambridge


University Press, 1994.

4. Old R. W. and Primrose, S. B. 5thEdn. Principles of Gene


manipulation: An introduction to genetic engineering, 3rd edition,
Black well Scientific Publications, 1994

5. Lewin B. Genes VIII, New York, Oxford University Press, 2003

6. Christopher H., Gene cloning and Manipulating, Cambridge


University Press, 1995.

7. Watson J. D., Baker T. A, Bell S. P., Gann A. N., Levine M and Losick
R., Molecular biology of the gene, 5th edition, Cold Spring Harbor
Laboratory Press, 2003.

8. Glick B. T and Pastermak J.J, Molecular Biotechnology: Principles


and application of recombinant DNA, Washington D.C: ASM press,
1998.

Evaluation Pattern
CHE431 - CHEMISTRY - IV ORGANIC AND
PHYSICAL CHEMISTRY (2011 Batch)
No of Lecture
Total Teaching Hours for Semester:60
Hours/Week:4
Max Marks:100 Credits:3
Course Objectives/Course Description
This course deals with the additional concepts of Organic and Physical
Chemistry and lays the foundation for more advanced topics in the subsequent
courses.
Learning Outcome
Students gain insight into the rates of reactions and factors that affect it;
Unit-1 Teaching Hours:8
Arenes and Aromaticity

Nomenclature of benzene derivatives. The aryl group- aromatic nucleus and


side chain; Aromaticity- the Huckel rule, aromatic ions. Aromatic
electrophilic substitution, general pattern of the mechanism- Mechanism of
nitration, sulphonation and Friedel-Crafts reactions; Energy profile
diagrams. Activating and deactivating substituents. Orienting and activity
influence of substituents in toluene, chlorobenzene, nitrobenzene and
phenol; hyperconjugation and resonance effects of these groups.

Naphthalene-structural elucidation; anthracene and phenanthrene-resonance


structures; Carcinogenicity.
Unit-2 Teaching Hours:3
Alkyl and Aryl Halogen Compounds

Relative reactivities of alkyl halides versus allyl, vinyl and aryl halides.
SN1 and SN2 mechanisms. Elimination reactions – E1 and E2 mechanisms,
Saytzeff elimination. Side chain chlorination of toluene. DDT and BHC.
Nucleophilic aromatic substitution in aryl halides.
Unit-3 Teaching Hours:2
Organometallic Compounds
Organo magnesium compounds: the Grignard reagents-formation, structure
and chemical reactions.

Organo lithium compounds: formation and chemical reactions.


Unit-4 Teaching Hours:5
Alcohols x
Monohydric alcohols - methods of formation - reduction of aldehydes,
ketones, carboxylic acids and esters, hydrogen bonding, acidic nature.

Dihydric alcohols - methods of formation, vicinal and geminal glycols -


pinacol-pinacolone rearrangement, oxidation of glycols by periodic acid,
lead tetra acetate.

Trihydric alcohols - methods of formation, chemical reactions of glycerol.


Unit-5 Teaching Hours:2
Phenols
General methods of preparation and reactions. Relative acidity of phenols,
alcohols and carboxylic acids. Reimer-Tiemann and Kolbe reactions.
Structure of dihydric and trihydric phenols.
Unit-6 Teaching Hours:2
Ethers and Epoxides
Methods of formation and chemical reactions of ethers, Ziesel’s method.
Synthesis of epoxides. Acid and base catalyzed ring opening of epoxides.
Unit-7 Teaching Hours:5
Aldehydes and Ketones-5 Hrs
Synthesis of aldehydes from acid chlorides, synthesis of aldehydes and ketone using
1,3-dithianes, synthesis of ketones from nitriles and from carboxylic acids.
Mechanism of nucleophilic additions to carbonyl group with particular emphasis on
benzoin, aldol, Perkin and Knoevengel condensations. Cannizzaro’s reaction.
Meerwin-Pondorof- Verly, Clemmensen, Wolff-Kishner, LiAIH4 and NaBH4
reductions
Unit-8 Teaching Hours:3
Organic Synthesis via Enolates
Acidity of α-hydrogens, synthetic applications of diethyl malonate and ethyl
acetoacetate. Synthesis of ethyl acetoacetate, Claisen condensation. Keto-
enol tautomerism in ethyl acetoacetate.
Unit-9 Teaching Hours:8
Chemical Kinetics- 8 Hrs
Chemical kinetics and its scope; rate of a reaction, factors influencing the
rate of a reaction – concentration, temperature, pressure, solvent, light,
catalyst; concentration dependence of rates, mathematical characteristics of
simple reactions – zero order, first order, second order, half-life and mean
life. Determination of the order of reaction – differential method, method of
integration, method of half life period and isolation method.

Radioactive decay as a first order phenomenon.

Theories of chemical kinetics: Effect of temperature on rate of reaction,


Arrhenius equation, concept of activation energy.

Simple collision theory based on hard sphere model, transition state theory
(equilibrium hypothesis), Expression for the rate constant based on
equilibrium constant and thermodynamic aspects. Fast reactions – Flash
photolysis. Parallel and consecutive reactions – examples. Third order
reactions, Expression for k (derivation not required).
Unit-10 Teaching Hours:8
Liquid mixtures
Completely miscible, partially miscible and immiscible pairs of liquids,
Raoult’s law, Ideal and non ideal solutions. Vapour pressure-composition
and boiling point- composition curves. Principle of fractional distillation.
Fractional distillation of completely miscible pair of mixtures, azeotropic
mixtures, Lever rule.

Study of partially miscible pair of liquids. Concept of upper and lower


critical solution temperature. Effect of addition of salt on C.S.T. of water
and phenol. Steam distillation and applications.
Distribution law- verification and application, Henry’s law of gas solubility
and its applications.
Unit-11 Teaching Hours:8
Chemical Thermodynamics-(A macro
approach) -8 Hrs

Need for the second law of thermodynamics – different ways of stating


second law. Heat engines. Carnot cycle and its efficiency, calculation of
entropy changes in different processes, free energy, work function and
chemical potential. Criteria for equilibrium and spontaneous processes.
Thermodyanmic principles in Ellingham’s diagram.

Variation of free energy with pressure and temperature. Van’t Hoff


Isotherm. Gibbs-Helmholtz equation, Van’t Hoff isochore. Clausius-
Clapeyron equation and its applications. Zeroth law of thermodynamics.

Qualitative treatment of Nernst heat theorem. Third law of thermodynamics


and concept of residual entropy.Partial molar properties – fugacity.
Unit-12 Teaching Hours:6
Crystallography
Definition of space lattice, unit cell. Laws of crystallography, Symmetry
elements in crystals, seven crystal systems, Weiss and Miller indices, X-ray
diffraction of crystals, derivation of Braggs equation, Determination of the
structure of NaCl by rotating crystal method.

Elementary discussion of the liquid crystalline state: Classification,


structure and applications.
Text Books And Reference Books:

Arun Bahl And B.S.Bahl A Text Book Of Organic Chemistry, 17th Edition, New
Delhi: S.Chand&Company, 2005

Atkins, P.W., Physical chemistry, 5th ed., Oxford University Press, 1992.
Essential Reading / Recommended Reading

1. Morrison R. T. and BoydR. N.Organic Chemistry, 6th Edition, New


Delhi: Prentice-Hall of India (P)Ltd., 1992.

2. Mukherji S. M., Singh S. P., and KapoorR. P.Organic Chemistry, 5th


Edition, New Delhi: New Age International (P) Ltd. Publishers,b1999.
3. Finar I. L.,Vol. II, Organic Chemistry, 5th Edition, Reprinted in1996,
New Delhi: ELBS and LongmanLtd., 1975.

4. SykesP.A Guide Book to Mechanism in Organic Chemistry, 6th Edition,


New Delhi: Orient Longman Ltd., 1997.

5. Seyhan N. Ege, Organic Chemistry – Structure and Reactivity, Delhi:


AITBS publishers, 1998.

6. Paula Y. Bruice, Organic Chemistry, 2nd Edition, Prentice-Hall


International Inc, New Jersey International Edition, 1998.

7. Arun Bahl And B.S.Bahl A Text Book Of Organic Chemistry, 17th


Edition, New Delhi: S.Chand&Company, 2005.

8. Atkins, P.W., Physical chemistry, 5th ed., Oxford University Press,


1992.

9. BarrowG.M., Physical chemistry, 5th ed., Mc Graw Hill,1986.

10 .Daniels F. and Alberty F.A., Physical chemistry 4th


edition,Wiley,1992.

11. Samuel Glasstone, Textbook of Physical Chemistry , 2nd edition,


Mcmillan,1998.

Evaluation Pattern
BTY531 - IMMUNOLOGY (2010 Batch)
No of Lecture
Total Teaching Hours for Semester:45
Hours/Week:3
Max Marks:100 Credits:2
Course Objectives/Course Description
This paper will examine cellular and molecular aspects of the immune system.
Topics include immunogenetics and molecular structure of immunoglobulins, T
cell & B cell development, MHC antigens, modern vaccines, functions and
dysfunctions of the components of the immune system; applications of
immunological technologies in modern scienti ic research and development.
These topics will help the students to absorb most of the fundamentals in
immunology and this can bene it in understanding the advanced topics in this
area.
Learning Outcome
Students understand the various immune cells, molecules and pathways
involved in induction and regulation of innate and adaptive response. Students
learn immune techniques involved in detection and quanti ication of antigens
and antibodies. They are equipped to integrate the information on role of
immune system in hypersensitive reactions and apply this knowledge to
develop vaccines and new therapeutics.
Unit-1 Teaching Hours:6
Immune System
History of Immunology, Innate Immune response and its role in protection,
physiological barriers, mechanical barriers, chemical barriers. Adaptive Immune
response – naturally acquired and arti icially acquired immune response,
humoral and cellular component of the Immune response
Unit-2 Teaching Hours:5
Cells and organs of immune system
Role of different blood cells in immune system, primary lymphoid organs-
thymus, nude mice, bone marrow, secondary lymphoid organs- spleen, lymph
node, MALT.
Unit-3 Teaching Hours:5
B-cell and T-cell development
Activation of B cells and T cells-maturation, proliferation and differentiation,
clonal selection, B-cell receptors, T Cell receptor, αβ T cells, γδ T cells, TCR-CD
complex, Structure of TCR and its interaction with MHC-I and MHC-II peptide
Complex - T cell selection, Autoimmunity.
Unit-4 Teaching Hours:3
Antigens
Antigenicity and Immunogenicity, epitopes of B Cells and T Cells, Haptens.
Unit-5 Teaching Hours:4
Antibodies
Elucidation of antibody structure, variable regions, constant regions, Heavy
chains light regions, classi ication of antibodies (IgA, IgG, IgM, IgDIgE).
Functions of different antibodies, generation of antibody diversity, Monoclonal
antibodies-hybridoma technology
Unit-6 Teaching Hours:6
Antigen-antibody interaction
Af inity and avidity, precipitation reactions- radial immunodiffusion, double
immunodiffusion, Agglutination- heamagglutination, agglutination inhibition,
rocket electrophoresis, radioimmunoassay, ELISA- indirect, sandwich,
competitive ELISA , immuno luoroscent techniques
Unit-7 Teaching Hours:3
Major histocompatibily complex
MHC molecules and organization of their genes, Structure and function of MHC
types. Antigen processing, role of MHC in antigen presentation
Unit-8 Teaching Hours:4
Complement system
History and De inition of complement proteins, functions of complement
system, Classical pathway, Alternate pathway, Mannan binding lectin pathway
Unit-9 Teaching Hours:4
Hypersensitivity reactions
De inition o hypersensitivity reactions,Coomb’s classi ication of HS reactions-
Immediate reactions-Type I, II, III.Delayed HS reactions-type IV.
Unit-10 Teaching Hours:5
Vaccines and Immunization:
Passive and Active immunization. Types of Vaccines-Live, attenuated vaccines
,Inactivated vaccines ,Subunit vaccines, Toxoid vaccines, Conjugate vaccinesDNA
vaccines, Recombinant vector vaccines
Text Books And Reference Books:

1. Ivan Roitt, Essential Immunology.10th Ed. USA: Blackwell Company


Ltd, 2001.
2. Khan Falim H, The elements of Immunology. 1st Edn, India: Pearson
Education, 2009.

Essential Reading / Recommended Reading

1. Thomas J. Kindt, Barbara A. Osborne, Richard A. Goldsby,Kuby,


Immunlogy.6th Edn, USA: W.H. Freeman & Company, 2007.
2. Werner Luttman, Immunology. 2nd Edn, USA: Academic press, 2006.
3. David Male, Immunology. 7th Edn, USA: Mosby Elsevier, 2006.

Evaluation Pattern
BTY532 - ANIMAL CELL CULTURE AND PLANT
TISSUE CULTURE (2010 Batch)
Total Teaching Hours for Semester:45 No of Lecture Hours/Week:3
Max Marks:100 Credits:2
Course Objectives/Course Description
After an exposure to the basics of cell and molecular biology and the
techniques of gene manipulation, the students should be exposed to methods
for isolating/culturing the animal and plant tissue/cells. This paper emphasizes
on the basic tissue culture techniques and deals with all the basic methods and
variants of both plant animal tissue/cell cultures. A few of the advanced topics
like stem cell technology has been included which describes types of stem cells,
applications and ethics which makes the students have a feel of what’s
happening in this ield, currently.
Learning Outcome
Students learn the importance of animal and plant cell culture for research and
Biotechnology industry. Students connect the use of mammalian cells and plant
cells for industrial production of pharmaceutical and industrially important
products. They also learn the importance of sterilization, media nutrition and
parameters like pH, temperature on the growth of cells. Students learn different
techniques of scaling up the cultures for large scale production of products.
Unit-1 Teaching Hours:1
Introduction
History and scope of animal cell and tissue culture, advantages and
disadvantages of tissue culture
Unit-2 Teaching Hours:3
Stimulating the Natural Conditions
Medium (De ined and unde ined), Growth factors promoting proliferation of
animal cell culture- EGF,FGF,PDGF,TGF,IL, IGF. Substrate, Gas phase
Unit-3 Teaching Hours:3
Establishment of cell line
Primary culture, Disaggregation of tissue (physical, chemical methods),
secondary cell lines, continuous cell lines, characteristics of cell lines,
Maintenance of cell lines, Measurement of cell viability - Evan’s blue method,
Trypan blue method.
Unit-4 Teaching Hours:3
Scaling Up
Anchorage dependent cell lines- Roux bottle, Roller bottle, Multiple array,
anchorage independent cell lines- Stirred bioreactor, airlift bioreactor,
immobilized bioreactor.
Unit-5 Teaching Hours:3
Organ Culture
Plasma Clot, Raft methods, Agargel, Grid method. Tissue engineering:-arti icial
skin, arti icial cartilage
Unit-6 Teaching Hours:3
In vitro fertilization
Introduction in vitro fertilization of farm animals, induction of superovulation,
preparation and collection of oocytes, preparation of spermatozoa, in vitro
fertilization and development, embryo transfer & its advantages.
Unit-7 Teaching Hours:4
Embryonic stem cells
De inition and importance, Properties of stem cell, Embryonic stem cell culture,
stimulation of embryonic stem cells to differentiate, therapeutic adult stem cells
- source, differentiation, similarities between adult and embryonic stem cells,
applications, concept of cord blood banking, Bioethics.
Unit-8 Teaching Hours:1
Introduction
Totipotency of Plant cells, history of plant tissue culture
Unit-9 Teaching Hours:3
In vitro culture conditions
Nutrient media- major and minor nutrients, their role, Growth regulators
(Auxin, cytokinins,and gibberellins), aseptic techniques- sterilization &
disinfection, Sterilization of media, Air sterilization, Explant preparation.
Unit-10 Teaching Hours:4
Suspension culture
Isolation of single cell from orrgans (mechanical method, enzymatic method),
subculturing of suspension cultures, types of suspension culture, culture
medium for cell suspensions, synchronisation of Suspension Cultures, viability
of cultured cells (reduction of Tetrazolium salts, FluoresenceDiacetate method,
Evan’s Blue Method).
Unit-11 Teaching Hours:1
Single Cell Cultures
Techniques; Filter paper raft nurse technique, Bergmann technique,
Microchamber technique, applications.
Unit-12 Teaching Hours:2
In vitro fertilization
Type of Explant, Culture of Ovary and ovule, Factors affecting in vitro
pollination, applications of in vitro fertilization.
Unit-13 Teaching Hours:4
Organ culture
Anther culture- technique of androgenesis, factors in luencing anther culture,
haploids from isolated microspore, application of haploids in plant breeding.
Embryo Culture- types, nutritional requirements, applications. Endosperm
culture -triploid production- source material, nutrient media, Organogenesis,
applications in plant improvement.
Unit-14 Teaching Hours:2
Clonal Propagation of elite species
Micro propagation, explants for microprpagation, advantages of
micropropagation, stages, and limitations.
Unit-15 Teaching Hours:2
Somaclonal variation
Source material, culture conditions, molecular basis, isolation of variants,
application in plant breeding.
Unit-16 Teaching Hours:2
Disease Free Plants
Methods of virus elimination; Meristem tip culture, Thermotherapy, cryotherapy
and chemotherapy and meristem tip thermotherapy combo method, Otherin
vitro methods. Virus indexing- Sap Transmission Test, Serology
Unit-17 Teaching Hours:2
Somatic Embryogenesis
Somatic embryogenesis -selection of explants, embryo maturation and plantlet
development, practical applications, Arti icial seeds- preparation, applications
and arti icial seeds.
Unit-18 Teaching Hours:2
Protoplast culture
Isolation of protoplast- mechanical and enzymatic methods, viability of
protoplast, culture of protoplasts, regeneration, applications.
Text Books And Reference Books:

1. Razdan. .M. K..Introduction to Plant Tissue Culture, 2ndedn., New


Delhi: Oxford & IBH Publishing Co. Pvt Ltd, 2010.

2. Sathyanarayana .U. Biotechnology, Kolkota: Books and Allied (P) Ltd.,


2005.

3. Singh. B. D. Plant Biotechnology and Industrial Biotechnology, New


Delhi: Kalyani Publishers, 2005.

Purohit.S.S. Plant Tissue Culture. Jodhpur: Agrobios, 2008


Essential Reading / Recommended Reading

1. Butler. M. Animal Cell Culture And Technology, 2ndedn,, UK: Taylor &
Francis , 2004.
2. Freshney. I, .Culture Of Animal Cells. New York .John Wiley and Sons.
2006.

3. John R.W.Masters. Animal Cell Culture: A Practical Approach. USA:


Oxford University Press, 2000.
Evaluation Pattern
CHE532 - CHEMISTRY - VI PHYSICAL CHEMISTRY
(2010 Batch)

Total Teaching Hours for Semester:45 No of Lecture Hours/Week:3


Max Marks:100 Credits:2
Course Objectives/Course Description
The student learns the importance of physical chemistry in the present
world and gets encouraged to take up higher studies and a career in the
subject. Importance of conservation of energy and the use of
environmentally friendly energy sources are emphasized.

Learning Outcome
Study of topics like electrochemistry, spectroscopy etc. give a chance to have an analytical thinking and
an urge to develop more environmental friendly energy cells like fuel cells solar cells etc. it provides a
chance to think of improving the efficiency of these kind of cells and thus shows an environmental
concern towards the planet earth which we have to substance for posterity.

Unit-1 Teaching Hours:5


Chemical and Ionic Equilibria

Equilibrium constant and free energy. Ionic equilibria, common ion effect,
hydrolysis of salts. Relationship between Kh, Kw , Ka and Kb . Effect of
temperature and concentration on degree of hydrolysis. Evaluation of pH of
the solutions. Solubility product and its applications. Buffer solution and
mechanism of buffer action.
Unit-2 Teaching Hours:2
Adsorption

Freundlich’s adsorption and Langmuir’s adsorption isotherms, BET


equation (derivation not required) and its applications.
Unit-3 Teaching Hours:15
Electrochemistry
Definitions of specific, equivalent and molar conductances (k, l and m).Cell
constant. Methods of determination of specific conductance, evaluation of
equivalent and molar conductance-problems to be set only in SI units.
Variation of specific and equivalent/molar conductance with dilution.
Temperature dependence of ionic conductance. Strong and weak
electrolytes. Conductometric titrations (only acid-base type).

Transport number: Definition; Determination by moving boundary method.


Causes of abnormal transport numbers observed in certain systems.
Problems based on transport number.

Kohlrausch’s law and its applications: (1) Evaluation of L¥ from l+ and


l- (2) Evaluation of degree of dissociation of a weak electrolyte (3)
Evaluation of L¥ of a weak electrolyte (4) determination of solubility from
conductance of saturated solutions of sparingly soluble salts (AgCl, BaSO4).
Problems based on these.

Limitations of Arrhenius theory: Qualitative account of Debye-Huckel


theory, Debye-Huckel-Onsagar equation for aqueous solutions of 1:1
electrolytes.

Galvanic cells: Conventions of representing galvanic cells - reversible and


irreversible cells, derivation of Nernst equation for single electrode potential
(free energy concept). Weston-cadmium cell.Determination of emf of a cell.
Determination of E° of Zn/Zn2+ and Cu/Cu2+ electrodes. Liquid junction
potentials-elimination of liquid junction potential.

Types of electrodes: Metal and gas electrodes (chlorine), redox electrodes.


Reference electrodes: standard hydrogen electrode, calomel electrode.
Quinhydrone electrode, glass electrode, Determination of pH using calomel,
quin-hydrone and glass electrodes. Problems.

Concentration cells: (1) emf of concentration cells. (2) Determination of


solubility of sparingly soluble salts, problems. Redox electrodes: emf of
redox electrodes. Potentiometric titrationinvolving only redox systems.

Fuel cells, solar cells and solar energy panels.


Unit-4 Teaching Hours:15
Chemical Spectroscopy

Electromagnetic spectrum and the interaction of radiation with matter.


Regions of spectrum. Born-Oppenheimer approximation. Degrees of
freedom.

Origin of molecular spectra: Study of rotation, vibration spectra of


diatomic molecules. Expression for rotational energy. Evaluation of
internuclear distance from moment of inertia- problems. Criterion for
absorption of radiation - selection rule. Expression for potential energy of
simple harmonic oscillator–Hooke’s law. Expression for vibrational energy.
Zero point energy. Concept of force constant-its evaluation-problems.
Degrees of freedom-modes of vibration for CO2 and H2O molecules.
Vibration - rotation spectra PQR bands. Harmonic bands.

Concept of Polarisability. Raman spectra-qualitative study. Stokes and anti-


Stokes lines-selection rules. Advantages of Raman spectroscopy over IR
spectroscopy.

Electronic spectra: Potential energy curves for bonding and antibonding


orbitals. Electronic transitions, qualitative description of s, p and non-
bonding orbitals and transitions between them. Selection rules and Franck-
Condon principle.

Magnetic resonance spectroscopy

NMR spectroscopy, ESR spectroscopy and their applications. NQR


spectroscopy and Mossbaur spectroscopy. (Only principles to be
discussed).
Unit-5 Teaching Hours:8
Photochemistry
Laws of photochemistry: Grotthuss-Draper law, Stark-Einstein law,
Differences between photophysical and photochemical processes with
examples. Comparison of photochemical and thermal reactions.

Quantum yield of photochemical combination of (1) H2 and Cl2 (2) H2 and


Br2 (3) dissociation of HI (4) dimerisation of anthracene.
Photosensitization, photostationary equilibrium. Singlet and triplet states-
Fluorescence, Phosphorescence, Luminescence, Bioluminescence, chemical
sensors.

Beer-Lambert’s law: Applications. Problems on absorption coefficient and


molar extinction coefficient. Laser, classification and uses.
Text Books And Reference Books:

1.P.WAtkins., Physical chemistry by 5th ed., Oxford University Press,


1992.

2. G.M Barrow, Physical chemistry By., 5th ed.Mc Graw Hill,1986.

Essential Reading / Recommended Reading

3.F.Daniels, and F.A Alberty, Physical chemistry ,4th


edition,Wiley,1992.

4. GlasstoneSamuel, Textbook of Physical Chemistry ,2nd


edition,Mcmillan,1998.

5. GlasstoneSamuel, Electrochemistry for chemists, 2nd edition, East-


West.

Evaluation Pattern
BTY631 - BIOPROCESS ENGINEERING AND
ENVIRONMENTAL BIOTECHNOLOGY (2010 Batch)
Total Teaching Hours for Semester:45 No of Lecture Hours/Week:3
Max Marks:100 Credits:2
Course Objectives/Course Description
This paper covers important topics in the development, production, recovery,
and analysis of products produced by biotechnology. The course traces the path
of a biological product from the cell through the production facility, the inal
processing, and formulation. It discusses the growth characteristics of the
organisms used to produce biological compounds, the techniques used in
product recovery and puri ication analysis. The course emphasizes the use of
Good Laboratory Practices (GLP) in these analyses. The environmental
biotechnology portions includes the serious environmental issues of the society
and discusses the solutions to these problems using biotechnology
Learning Outcome
Students learn and understand cell growth, goals of fermentation and relate it
to a real-life example. Different types of bioreactor, auxiliary equipment and
applications are understood. Students understand how parameters such as pH,
temperature, aeration, and agitation that affects the fermentation. Students
learn the industrial production of certain metabolites and their recovery.
Students learn and assess biological methods for pollution control, energy and
resource recovery from waste, bioremediation and how they can contribute to
clean technology, Biological processes to remove or recover inorganic and
organic pollutants from liquid and gaseous ef luents, and from solid waste,
Biological processes for energy and biomass production from waste.
Unit-1 Teaching Hours:1
Introduction to Concepts of Bio process Engineering
De inition of a Bioprocess. An over view of bioprocesses with their various
components. Bioprocess operation & their global impact
Unit-2 Teaching Hours:3
Bioreactors: Construction, Design & Operation
Components of the fermenters & their speci ications, types of fermentors-
conventional stirred tank reactors, types of impellers, aeration, temperature
regulation, pH monitoring, antifoaming agents, airlift fermentors, bubble up
fermentors, luidized bed fermentors, packed bed fermentors, tower reactors,
drum reactors, photobioreactors
Unit-3 Teaching Hours:3
Strain improvement and Media
Need for strain development, Isolation of microbes (isolation from unusual
habitats, mutant selection (antibiotic resistance, anti-metabolite selection, and
auxotrophic selection), Genetic recombination methods in strain improvement.
Media for large-scale processes & their optimization: types of media, synthetic
and crude substrates, Constituents of media, Design of media, costing of media.
Unit-4 Teaching Hours:3
Air & Media sterilization
Air Sterilization priciples, Mechanisms of capture of particles in Air, Depth
& Screen Filters,Sizing, Testing & Vallidation of Filters for Air
Sterilization. Principle of Media Steriliztion, Batch & Continuous
sterilization techniques.
Unit-5 Teaching Hours:3
Types of fermentations and the process of
fermentation
Solid substrate fermentation, submerged fermentation- batch fermentation, fed-
batch fermentation, semi-continuous fermentation, continuous fermentation,
microbial growth in relation to substrate in all the above types. Fermentation
based on type of product formation- type I, II, III. Fermentation process-
inoculum preservation, inoculum build up, pre-fermentation, product
fermentation, recovery.
Unit-6 Teaching Hours:4
Product Recovery & Down Stream Processing in
Fermentation & Bioprocess Technology
Solid-liquid separation (Flocculation, Filtration, Centrifugation), Cell disruption
(Physcal, chemical and enzymatic), Extraction, Precipitation, Distillation,
Evaporation, Chromatographic separation, Adsorption, Concentration,
formulation- Lyophilization, spray drying
Unit-7 Teaching Hours:3
Enzyme Technology
Types of enzyme immobilization- Adsorption, Covalent binding, Entrapment,
encapsulation. Properties of immobilized enzymes ( Km, Ks, cycle time half life),
protein engineering, techniques of protein engineering, improvement in
catalytic ef iciency, applications of protein engineering, industrial applications
of enzymes in leather, textile, baking, detergent industries.
Unit-8 Teaching Hours:5
Microbial production of products and quality analysis
Classi ication of metabolic products- Primary, secondary and bioconversion
products. Production of alcoholic beverage (Beer), amino acid (glutamic acid),
antibiotic (penicillin) single cell protein, single cell oil. Quality Control, Quality
assurance, Standard Operating Procedures (SOP) & Good Manufacturing
Practices (GMP)
Unit-9 Teaching Hours:5
Alternate sources of fuels
Conventional fuels and their environmental impact, Modern fuels– Advantages
over conventional fuels. Methanogenic bacteria, Biogas, Microbial hydrogen
Production, gasohol, biodiesel
Unit-10 Teaching Hours:5
Bioremediation
Bioremediation of soil & water contaminated with oil spills, heavy metals and
detergents. Degradation of lignin and cellulose using
microbes.Phytoremediation.degradation of pesticides and other toxic chemicals
by micro-organisms, degradation of aromatic, chlorinated hydrocarbons and
petroleum products.
Unit-11 Teaching Hours:2
Waste Water Treatment
Treatment of municipal waste and Industrial ef luents using microbes.
Unit-12 Teaching Hours:5
Integration of genetic engineering & applied
microbiologyin Agriculture
Uses of genetically engineered microbes in Agriculture- biofertilizers
(rhizobium, mycorrhiza), nif gene cloning, biopesticides BT gene (cry gene)
technology
Unit-13 Teaching Hours:3
Bioleaching
Enrichment of ores by microorganisms (Gold, copper, and Uranium)
Text Books And Reference Books:
1. Sathyanarayana. U, Biotechnology. Books and Allied (P) Ltd,
Kolkota: 2008.

2. Jogdand S.N, Environmental Biotechnology. 3rd Edn, India: Himalaya


Publication House, 2001.

3. Singh B.D, Biotechnology. 2nd EdnNew Delhi: Kalyani Publishers,


2007.

Essential Reading / Recommended Reading

1. Whittaker &Stanbury, Principles of Fermentation Technology. 2nd


Edn, Edinburgh: Butterworth Heinemann Press,2003.

2. Pauline.M. Doran, Bioprocess Engineering Principles.


2ndEdn,London: Academic Press, 2000.

3. Zhong, Jian-Jiang, Biomanufacturing. New York: Springer-Verlag


Heidelberg, 2004.

4. Hui. Y.H, Lisabeth M. Goddik, AaseSolvejg Hansen, JytteJosephsen, Wai-Kit


Nip, Handbook of Food and Beverage Fermentation Technology. USA:
Marcel Dekker Inc, 2004.

5. David A. Mitchell, Nadia Krieger,Solid-state fermentation bioreactors:


Fundamentals of design and operation . Germany: Springer, 2006.
Evaluation Pattern
BTY632 - ANIMAL BIOTECHNOLOGY AND PLANT
BIOTECHNOLOGY (2010 Batch)
No of Lecture
Total Teaching Hours for Semester:45
Hours/Week:3
Max Marks:100 Credits:2
Course Objectives/Course Description
The paper describes the concepts of animal and plant biotechnology. The plant
biotechnology chapters will examine the scienti ic and technical advances which
underlie the production of genetically modi ied crops. Topics include: plant
genome organization and gene expression, genetic manipulation to confer
resistance to herbicides, pests and disease and strategies for engineering stress
tolerance and the improvement of crop yield and quality. Animal biotechnology
focuses on use of culturing animal cells for the production of sustainable
industrial products and inexpensive drugs for medical and veterinary use. It
also provides a forum for presenting and discussing current events in the ield
of biotechnology research.
Learning Outcome
Students learn the applications of plant genetic engineering and discuss ethical
and social issues regarding genetically-modi ied crops with respect to crop
improvement e.g. BT gene enhanced nutritional quality and molecular
pharming. Methods of plant transformation using Agrobacterium with
transferred DNA (T-DNA) from the tumour inducing (Ti) plasmid are
understood. Students learn biotechnological applications with their ethical,
social and environmental implications
Unit-1 Teaching Hours:5
Production of secondary metabolites
Expression of Cloned proteins in animal cell, High level production of transgene
products in animal cells, Human growth hormone, Human insulin, Factor viii,
Interferon, Tissue Plasminogen activator.
Unit-2 Teaching Hours:5
Vaccines and Therapeutic Agents
Recombinant vaccines, Sub unit vaccines – Hepatitis B, Foot and mouth disease,
Herpes simplex virus vaccines their production, DNA vaccines, Advantages and
disadvantages RNA vaccine, Production of viral vaccine, Recombinant vaccine
against vaccinia virus
Unit-3 Teaching Hours:6
Transgenic Animals
Objective of Gene Transfer, Gene construction, methods- Retroviral Vector
method , DNA microinjection method, Engineered embryonic stem cell method,
Detection of transgenes, Transgenic mice and their applications, Human mice,
Alzheimer’s mouse, Onco mouse, Knock out mouse, SCID mouse, Dolly-
Transgenic sheep, Animal bioreactors and their Importance.
Unit-4 Teaching Hours:5
Gene therapy
Approaches for gene therapy, ex vivo gene therapy, In vivo gene therapy,
Antisense Therapy, gene therapy for Adenosine deaminase, Hemophilia, Familial
hyper Cholesterolemia.
Unit-5 Teaching Hours:4
Monoclonal antibodies
Creation of hybridoma cells, Production of Monoclonal Antibodies, Large scale
production, Human monoclonal antibodies, Genetic engineering strategies for
monoclonal antibodies, Human-mouse antibodies, Advantages and limitations of
monoclonal antibodies
Unit-6 Teaching Hours:5
Genetic engineering in plants
Gene construction, vectors for transgenic plants-Plasmid vectors, Plant virus
vectors, Transformation Techniques-Agrobacterium mediated gene transfer,
Direct gene method), Integration of transgene, Selection Markers\Reporter
genes, Hybrid & Cybrids - Protoplast isolation, Protoplast fusio, Selection of
Hybrids & Cybrids, Applications
Unit-7 Teaching Hours:4
Suppression of endogenous genes:
Antisense gene approach, Co-suppression of genes, Terminator gene technology
Unit-8 Teaching Hours:4
Transgenic crops with new traits
Herbicide tolerance, Insect and Disease resistance, Nutrient quality (golden
rice), Post harvest quality traits.
Unit-9 Teaching Hours:2
Plant derived Vaccines
Edible vaccines, Recombinant and subunit vaccines, Status of Plant derived
Vaccines.
Unit-10 Teaching Hours:5
Secondary metabolites
Introduction, mass production of plant secondary metabolites using Suspension
culture,&Immobilised Plant cells, strategies to improve the production of
secondary metabolites, production of Carotenoids, Capsaicin, Anthocyanin.
Text Books And Reference Books:

1. Mahesh. S, Plant Molecular Biotechnology. New Delhi: New Age


International Publishers, 2008.
2. Sathyanarayana .U. Biotechnology. Kolkota: Books and Allied (P)
Ltd., 2005.
3. Keshavachandran. R and Peter. K.V. Plant Biotechnology: Methods in
Tissue Culture and Gene Transfer. Hyderabad, Orient Blackswan.
2008
4. Singh. B. D. Plant Biotechnology and Industrial Biotechnology, New
Delhi: Kalyani Publishers, 2005

Essential Reading / Recommended Reading

1. Slater A. Plant Biotechnology. Oxford University Press. 2007


2. Butler M. Animal Cell Culture and Technology. England: Bios
Scientific Publishers. 2004.
3. Jenkins N. Animal Cell Biotechnology, New York, Humana Press.
2007.
4. Ralf P. Animal Cell Biotechnology: Methods and Protocols. New York:
Humana Press. 2007.

Evaluation Pattern
BTY652 - INTERNAL PROJECT (2010 Batch)
Total Teaching Hours for Semester:30 No of Lecture Hours/Week:2
Max Marks:50 Credits:1
Course Objectives/Course Description
With the guidance from the faculties, the students are given freedom to design
and perform a project work of their choice pertaining to the different ields in
Biotechnology. The main intention of this project is to develop research attitude
and aptitude in students so that they can analyze their own interests in different
ields.

Learning Outcome
Project work helps them to make use of different techniques that they have
covered in the previous semesters. This may even help the students to choose
their future courses for their higher studies, based on their interests. It also
helps the students to plan up and execute the research work their own.
Students will get exposure to the process of reviewing the literature and also
the scienti ic writing.
Unit-1 Teaching Hours:30
The assessment of the project work will be done in different levels.

1. Internal presentation by the students in the department and


project work done on weekly basis
2. Project result and report

3. Final presentation by the student in front of external examiners.

Text Books And Reference Books:

Green M. R., and Sambrook R., Molecular Cloning: A Laboratory


Manual. 3rd edition, New York, USA: Cold spring Harbor Laboratory
Press, 2002.

Essential Reading / Recommended Reading


Naik G. R., Introduction to Basic Molecular Biology Techniques, Himalaya
Publishing House, Mumbai. 2004
Evaluation Pattern

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