Dissolved Oxygen: Title
Dissolved Oxygen: Title
Dissolved Oxygen: Title
TITLE
To determine the amount of Dissolved Oxygen (DO) in water.
DISSOLVED OXYGEN
The dissolved oxygen (DO) is oxygen that is dissolved in water. The oxygen dissolves by
diffusion from the surrounding air; aeration of water that has tumbled over falls and rapids;
and as a waste product of photosynthesis. A simplified formula is given below:
CO2 H 2O O2 C6H12O6
Fish and aquatic animals cannot split oxygen from water (H2O) or other oxygen-containing
compounds. Only green plants and some bacteria can do that through photosynthesis and
similar processes. Virtually all the oxygen we breathe is manufactured by green plants. A
total of three-fourths of the earth’s oxygen supply is produced by phytoplankton in the
oceans.
• Fish, invertebrates, plants, and aerobic bacteria all require oxygen for respiration.
• Much of the dissolved oxygen in water comes from the atmosphere. After dissolving at
the surface, oxygen is distributed by current and turbulence. Algae and rooted aquatic
plants also deliver oxygen to water through photosynthesis.
• The main factor contributing to changes in dissolved oxygen levels is the build-up of
organic wastes. Decay of organic wastes consumes oxygen and is often concentrated in
summer, when aquatic animals require more oxygen to support higher metabolisms.
• Depletions in dissolved oxygen can cause major shifts in the kinds of aquatic organisms
found in water bodies.
• Temperature, pressure, and salinity affect the dissolved oxygen capacity of water. The
ratio of the dissolved oxygen content (ppm) to the potential capacity (ppm) gives the
percent saturation, which is an indicator of water quality.
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METHODS OF DETERMINATION OF DO IN WATER:
(1) Winkler Method
(2) Azide modification of Winkler Method
(3) Rideal Stewart method
(4) DO-meters
Then by titration with S2O3-2, thiosulphate ion (thiosulphate ion comes from sodium
thiosulphate), iodine can be calculated.
2S2O3-1 + I2 S4O6-2 + 2I-1
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PROCEDURE
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OBSERVATIONS AND CALCULATIONS
DISSOLVED OXYGEN
Volume of
Sample Dissolved Mean Dissolved
Sample Sample Titrant
Volume Oxygen (DO) Oxygen (DO)
No. Description (Na2S2O3)used
(ml) (mg/lit) (mg/lit)
(ml)
200
1 Lab Prepared
100
200
2 Lab Prepared
100
3 Lab Prepared
Comments:
Questions:
1) What are the factors upon which solubility of oxygen depends?
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4) What type of titration is involved in above test?
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