O LEVEL BIOLOGY OCT/NOV 2020 PAPER 32 (5090 - w20 - QP - 32)

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* 9 9 2 1 2 9 0 1 8 1 *
BIOLOGY 5090/32
Paper 3 Practical Test October/November 2020
1 hour 15 minutes
You must answer on the question paper.
You will need: The materials and apparatus listed in the confidential instructions
INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, centre number and candidate number in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
● You should show all your working and use appropriate units.
INFORMATION
● The total mark for this paper is 40.
● The number of marks for each question or part question is shown in brackets [ ].
For Examiner’s Use
Total
This document has 8 pages. Blank pages are indicated.
DC (CE/SG) 180193/3
© UCLES 2020 [Turn over
2
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In order to plan the best use of your time, read through all the questions on this paper
carefully before starting work. rebrand.ly/COALIresources
1 When a seed germinates, the stem of the seedling grows upwards.
t.me/COALItelegram
You are going to investigate differences between seedlings grown in the light and seedlings grown
in the dark.
You are provided with approximately 15 seedlings grown in the light and 15 grown in the dark.
(a) (i) Examine the seedlings that have been provided. Complete the table to compare the leaf
colour, leaf size and stem length of seedlings grown in the light and in the dark.
feature seedlings grown in light seedlings grown in dark
leaf colour
leaf size
stem length
[3]
(ii) Select five seedlings grown in the light and five grown in the dark.
Measure the length of each stem to the height of the lowest leaf.
Record your measurements in the table below and calculate the mean lengths.
stem length in stem length in

seedling
light / mm dark / mm
1
2
3
4
5
mean length / mm
[4]
(b) Suggest why the growth observed in the dark might be an advantage to a plant in a shady,
forest environment.
...................................................................................................................................................
............................................................................................................................................. [1]
© UCLES 2020 5090/32/O/N/20

3
(c) Some scientists wanted to investigate the effect of fertiliser on the growth of wheat. Wheat
seeds were planted in soil to which nitrogen fertiliser had been added. rebrand.ly/COALIresources
After the seedlings had
grown to a height of 8 cm, they were measured at regular intervals.
The mean heights of the plants are shown in the table. t.me/COALItelegram
mean height of
time / days
plants / cm
0 8
10 10
30 29
60 58
90 73
110 73
(i) Construct a line graph of the data on the grid below. Join your points with ruled, straight
lines.
[4]
(ii) Use your graph to find the mean height of the plants at 50 days. Show your working on
the graph.
mean height ................................. [2]
© UCLES 2020 5090/32/O/N/20 [Turn over

4
(iii) Describe how the rate of growth of these plants changed between days 0 and 110.
...........................................................................................................................................
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...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [3]
(iv) Describe how to complete this investigation to find whether fertiliser does have an effect
on the rate of growth of wheat.
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [2]
(v) Increase in height was used as a measure of growth. Suggest two other plant features
that could have been used to measure growth.
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [2]
[Total: 21]
© UCLES 2020 5090/32/O/N/20

5
2 Milk contains casein, a protein that gives it a white colour. You are provided with a solution of
powdered milk.
• Mark a cross (X) on the outside of the base of a small, empty beaker. rebrand.ly/COALIdiscord
• t.me/COALItelegram
Place this beaker on the white tile so that you can see the X clearly when you look into the
beaker. rebrand.ly/COALIshitposting
You are going to add milk to the beaker as you look down on the X until the X is no longer visible.
You will then record the volume of milk you have added.
You will need to work out how you will measure this volume before you do this.
• Add milk slowly to the beaker as you look down on the X until the X is no longer visible.
(a) (i) Measure and record the volume of milk you added to the beaker.
........................ cm3 [1]
(ii) Describe how you measured this volume.
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [2]
• Use the dropper provided to add dilute hydrochloric acid (HCl ) to the milk in the beaker drop
by drop until the X becomes visible. Take care when handling hydrochloric acid.
(iii) Describe what happens to the milk as the X becomes visible.
...........................................................................................................................................
..................................................................................................................................... [1]
(b) Some protease enzymes can digest casein in milk and the milk becomes colourless. Not all
protease enzymes can digest casein.
A fruit contains a protease enzyme. Using a method similar to the one above, design an
investigation to find out whether this fruit protease will digest casein.
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
............................................................................................................................................. [4]
[Total: 8]
© UCLES 2020 5090/32/O/N/20 [Turn over
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3 The photograph shows the bone from the upper leg (between the hip and rebrand.ly/COALIbackup
knee) of two different
adult humans. The bones are labelled C and D. rebrand.ly/COALIresources
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C D magnification ×0.17
(a) Use the table to describe two differences between bones C and D as they appear in the
photograph.
bone C bone D
[2]
© UCLES 2020 5090/32/O/N/20

7
(b) Make a large drawing of bone C in the space below. rebrand.ly/COALIbackup
t.me/COALItelegram
[5]
(c) (i) On the photograph measure the length of bone D.
........................ mm [1]
(ii) Use your measurement to calculate the actual length of bone D.
Show your working.
........................................................... [3]
[Total: 11]
© UCLES 2020 5090/32/O/N/20
8
BLANK PAGE rebrand.ly/COALIbackup
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Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every
reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.
To avoid the issue of disclosure of answer-related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge
Assessment International Education Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download
at www.cambridgeinternational.org after the live examination series.
Cambridge Assessment International Education is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of the University of
Cambridge Local Examinations Syndicate (UCLES), which itself is a department of the University of Cambridge.
© UCLES 2020 5090/32/O/N/20

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rebrand.

You must answer on the question paper.

For Examiner’s Use

This document has 8 pages. Blank pages are indicated.

feature seedlings grown in light seedlings grown in dark

stem length in stem length in

© UCLES 2020 5090/32/O/N/20

mean height ................................. [2]

© UCLES 2020 5090/32/O/N/20 [Turn over

© UCLES 2020 5090/32/O/N/20

........................ cm3 [1]

(ii) Describe how you measured this volume.

(iii) Describe what happens to the milk as the X becomes visible.

© UCLES 2020 5090/32/O/N/20

(c) (i) On the photograph measure the length of bone D.

(ii) Use your measurement to calculate the actual length of bone D.

Show your working.

© UCLES 2020 5090/32/O/N/20

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