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    25 Caffeine

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    Dilawar Bakht
    This procedure outlines the testing of caffeine anhydrous raw material to ensure it meets specifications. The quality control manager and assistant are responsible for implementing the procedure, which involves physical inspection, identification through infrared spectroscopy, melting point determination, and UV spectroscopy. Key tests include loss on drying (max 0.5%) and assay (98.5-101.5% purity as determined by UV spectroscopy comparison to a standard). Safety precautions like masks, gloves, and separate waste disposal are also outlined.

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    25 Caffeine

    Uploaded by

    Dilawar Bakht
    35 views2 pages
    This procedure outlines the testing of caffeine anhydrous raw material to ensure it meets specifications. The quality control manager and assistant are responsible for implementing the procedure, which involves physical inspection, identification through infrared spectroscopy, melting point determination, and UV spectroscopy. Key tests include loss on drying (max 0.5%) and assay (98.5-101.5% purity as determined by UV spectroscopy comparison to a standard). Safety precautions like masks, gloves, and separate waste disposal are also outlined.

    Original Description:

    Complete test method of Caffeine

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    This procedure outlines the testing of caffeine anhydrous raw material to ensure it meets specifications. The quality control manager and assistant are responsible for implementing the procedure, which involves physical inspection, identification through infrared spectroscopy, melting point determination, and UV spectroscopy. Key tests include loss on drying (max 0.5%) and assay (98.5-101.5% purity as determined by UV spectroscopy comparison to a standard). Safety precautions like masks, gloves, and separate waste disposal are also outlined.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    35 views2 pages

    25 Caffeine

    Uploaded by

    Dilawar Bakht
    This procedure outlines the testing of caffeine anhydrous raw material to ensure it meets specifications. The quality control manager and assistant are responsible for implementing the procedure, which involves physical inspection, identification through infrared spectroscopy, melting point determination, and UV spectroscopy. Key tests include loss on drying (max 0.5%) and assay (98.5-101.5% purity as determined by UV spectroscopy comparison to a standard). Safety precautions like masks, gloves, and separate waste disposal are also outlined.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 2

    1.

    0 Purpose:-
    To provide a system for monitoring and measurement of Raw Materials and to ensure that the Raw
    Material conforms to the requirements of the defined Raw Material Specifications.

    2.0 Scope:-
    This procedure is applicable to the testing of Caffeine anhydrous only.

    3.0 Responsibility:-
    3.1. Quality Control Manager is responsible for implementation of this procedure.
    3.2. Assistant Quality Control Manager and Quality Control Analyst are responsible for practicing
    this procedure.
    4.0 HSE Requirements :-
    4.1. Wear safety masks, goggles and rubber gloves where necessary.
    4.2. Hand over wastes separately to sanitation section for safe disposal.
    4.3. To follow the safety rules and regulation according to procedure # QCD/QSP/002
    4.4. Store and handle the hazardous material according to procedure # QCD/QSP/005
    4.5. Handle the spillage material according to procedure # QCD/QSP/003

    5.0 Procedure:-
    5.1. Instrument:
    5.1.1. UV Spectrophotometer.
    5.1.2. Analytical Weighing Balance.
    5.1.3. Melting Point Apparatus
    5.1.4. FTIR
    5.1.5. Oven

    5.2. Glassware’s / Plastic ware’s


    5.2.1. Pipettes, Beaker, Volumetric Flask, Pipette suckers, Filter papers

    5.3. Physical Inspection:


    5.3.1. Appearance: A white, crystalline powder or silky, white crystals, sublimes readily.
    5.3.2. Solubility: Sparingly soluble in water, freely soluble in boiling water, slightly soluble
    in ethanol. It dissolves in concentrated solutions of alkali benzoates or salicylates.

    C8H10N4O2  194.2
    5.4. Identification:
    Infrared Absorption: The Infrared absorption spectrum of the sample corresponds to the
    reference spectrum of working standard of Caffeine Anhydrous.
    Melting Point: 2340 C to 2390 C.
    Ultraviolet Absorption: The UV-Visible absorption spectrum (scanned between 200nm and
    320nm) of sample solution corresponds to the reference spectrum of Caffeine working
    standard as prepared for assay.

    5.5. Loss on Drying: Not more than 0.5 per cent, determined on 1.000 g by drying in an oven at
    800 C for 4 hrs.

    5.6. Assay:
    5.6.1. Sample preparation: Dissolve 50.0mg of Caffeine sample in water and dilute to
    100ml with the same solvent. Dilute 2ml of the resultant solution to 100ml with water.
    5.6.2. Standard preparation: Dissolve 50.0mg of Caffeine standard in water and dilute to
    100ml with the same solvent. Dilute 2ml of the resultant solution to 100ml with water.
    Measure the absorbance of both sample and standard solution at 273nm using water
    as a blank.

    where;
    As = Absorbance of sample solution.
    Ar = Absorbance of standard solution.
    Wr = Weight of standard in mg.
    Ws = Weight of sample in mg.
    LOD = Loss on drying (%).
    P = Percentage Purity of standard on as is basis

    5.6.3. Limit: 98.5% to 101.5%

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