User Manual 01/2011 Edition 1: © HACH Company, 2011. All Rights Reserved. Printed in Germany

DOC022.53.
90323
DR 3900
USER MANUAL
01/2011 Edition 1
© HACH Company, 2011. All rights reserved. Printed in Germany.

Table of Contents
Section 1 Specifications ........................................................................................................................ 9

Section 2 General Information............................................................................................................. 11
2.1 Safety information............................................................................................................................. 11
2.1.1 Precautionary labels ................................................................................................................ 11
2.1.2 RFID module (not available on all models) .............................................................................. 12
2.1.3 Chemical and Biological Safety ............................................................................................... 12
2.2 Overview of product.......................................................................................................................... 13
Section 3 Installation ............................................................................................................................ 15
3.1 Unpack the instrument...................................................................................................................... 15
3.2 Operating environment ..................................................................................................................... 15
3.3 Front and back view.......................................................................................................................... 16
3.4 Power connections ........................................................................................................................... 17
3.5 Interfaces .......................................................................................................................................... 18
3.6 Cell compartments, cell adapters and light shield ............................................................................ 18
3.6.1 Cell compartments and adapters ............................................................................................. 18
3.6.2 Installation of cell adapter ........................................................................................................ 20
3.6.3 Use of the light shield for measurements................................................................................. 21
3.7 Beam path ........................................................................................................................................ 21
Section 4 Startup .................................................................................................................................. 23
4.1 Switch on the instrument, startup process........................................................................................ 23
4.2 Language selection........................................................................................................................... 23
4.3 Self-check ......................................................................................................................................... 23
4.4 Sleep mode....................................................................................................................................... 24
4.5 Power off the instrument................................................................................................................... 24
Section 5 Standard programs.............................................................................................................. 25
5.1 Overview........................................................................................................................................... 25
5.1.1 Tips for the use of the touch screen......................................................................................... 25
5.1.2 Use of the alphanumeric keypad ............................................................................................. 25
5.1.3 Main menu ............................................................................................................................... 26
3
Table of Contents
5.2 Instrument Setup ...............................................................................................................................27

5.2.1 Operator ID...............................................................................................................................27
5.2.1.1 Operator RFID tag (not available on all models) .............................................................28
5.2.2 Sample ID.................................................................................................................................29
5.2.2.1 Sample ID with scanner method 1...................................................................................30
5.2.2.2 Sample ID with scanner method 2...................................................................................30
5.2.3 Sample ID and operator ID with the optional RFID Sample ID Kit 
(not available on all models)........................................................................................................31
5.2.4 Security settings .......................................................................................................................31
5.2.4.1 Assign operator security level..........................................................................................33
5.2.4.2 Deactivate password .......................................................................................................34
5.2.5 Date and time ...........................................................................................................................35
5.2.6 Sound settings..........................................................................................................................36
5.2.7 PC and printer ..........................................................................................................................37
5.2.7.1 Printer setup ....................................................................................................................37
5.2.7.2 Print data .........................................................................................................................38
5.2.7.3 Print data continuously ....................................................................................................39
5.2.7.4 Network setup..................................................................................................................39
5.2.8 Power Management .................................................................................................................42
5.3 Save, recall, send and delete data ....................................................................................................43
5.3.1 The data log .............................................................................................................................43
5.3.1.1 Auto/manual data storage ...............................................................................................43
5.3.1.2 Recall stored data from the data log................................................................................44
5.3.1.3 Send data from the data log ............................................................................................45
5.3.1.4 Delete stored data from the data log ...............................................................................46
5.3.2 Control charts for data from the AQA Log ................................................................................46
5.3.3 Store, recall, send and delete data from wavelength scan and time course ............................48
5.3.3.1 Data storage from wavelength scan or time course ........................................................48
5.3.3.2 Recall stored data from wavelength scan or time course................................................49
5.3.3.3 Send data from wavelength scan or time course ............................................................49
5.3.3.4 Delete stored data from wavelength scan or time course ...............................................50
5.3.4 Data analysis............................................................................................................................51
5.3.4.1 Trends .............................................................................................................................51
5.3.4.2 Ratios ..............................................................................................................................53
4
Table of Contents
5.4 Stored Programs............................................................................................................................... 56

5.4.1 Select a saved test/method; entering user-specific basic data ................................................ 56
5.4.2 Stored programs options.......................................................................................................... 56
5.4.3 Use of program timers ............................................................................................................. 58
5.4.4 Set the dilution factor ............................................................................................................... 58
5.4.5 Run a standard adjust.............................................................................................................. 59
5.4.6 Set the chemical form .............................................................................................................. 59
5.4.6.1 Change of the default setting of the chemical form......................................................... 60
5.4.7 Run a reagent blank................................................................................................................. 60
5.4.8 To carry out a multiple determination....................................................................................... 61
5.4.9 Analysis of samples ................................................................................................................. 61
5.4.10 Update/edit tests (not available on all models) ...................................................................... 62
5.4.10.1 General advices for program update and edit............................................................... 62
5.4.10.2 Manual update of test data ........................................................................................... 62
5.4.10.3 Program a new test....................................................................................................... 63
5.4.11 Add stored programs to the favorite programs list ................................................................. 63
5.5 Barcode Programs............................................................................................................................ 64
5.5.1 Complete a barcode test.......................................................................................................... 64
5.5.2 Select the chemical evaluation form ........................................................................................ 65
5.5.2.1 Change of the default setting of the chemical form......................................................... 65
5.5.3 Basic test-specific and sample-specific data settings .............................................................. 65
5.5.4 Sample blank ........................................................................................................................... 67
5.5.5 Update/edit barcode tests ........................................................................................................ 67
5.5.5.1 Manual update of a barcode test .................................................................................... 67
5.5.5.2 Manual check/revision of test data.................................................................................. 70
5.5.5.3 Update via the Internet.................................................................................................... 70
5.5.5.4 Program a new test ......................................................................................................... 71
5
Table of Contents
Section 6 Expanded programs.............................................................................................................73

6.1 User programs...................................................................................................................................73
6.1.1 Program a user method............................................................................................................74
6.1.1.1 Single wavelength settings ..............................................................................................75
6.1.1.2 Multi wavelength settings ................................................................................................76
6.1.1.3 Calibration settings for single and Multi Wavelength mode.............................................78
6.1.1.4 Store a user program.......................................................................................................81
6.1.1.5 Additional user-defined parameters and functions ..........................................................81
6.1.2 "Free programming" program type ...........................................................................................82
6.1.2.1 Measurement process .....................................................................................................84
6.1.2.2 Enter a new element of a measuring sequence ..............................................................84
6.1.2.3 Enter the calibration formula (evaluation formula)...........................................................87
6.1.2.4 Enter variables.................................................................................................................90
6.1.2.5 Save a free programming user program..........................................................................90
6.1.3 Select a user program ..............................................................................................................90
6.1.4 Add, edit and delete user programs from the favorites list .......................................................90
6.1.4.1 Add to favourites..............................................................................................................91
6.1.4.2 Edit ..................................................................................................................................91
6.1.4.3 Clear Entry.......................................................................................................................92
6.2 Favorites ...........................................................................................................................................92
6.2.1 Recall a favorite program .........................................................................................................92
6.2.2 Delete a favorite program.........................................................................................................92
6.3 Standard Addition - monitoring/checking results ...............................................................................93
6.3.1 Complete a standard addition ..................................................................................................94
6.4 Single Wavelength (absorbance, concentration and transmittance readings) ..................................97
6.4.1 Set up Single Wavelength mode ..............................................................................................97
6.4.2 Take single wavelength readings (single reading) ...................................................................99
6.5 Multi Wavelength mode – readings with more than one wavelength ................................................99
6.5.1 Set the Reading mode at different wavelengths.......................................................................99
6.5.2 Complete a reading in the Multi Wavelength mode................................................................102
6.6 Wavelength Scan mode – recording of absorbance and transmission spectrums..........................102
6.6.1 Set up the wavelength scan ...................................................................................................103
6.6.2 Wavelength scan reading.......................................................................................................105
6.6.2.1 Navigation of the wavelength scan graph or a wavelength scan analysis.....................106
6.6.3 Work with reference scans .....................................................................................................106
6.7 Time course of absorbance/transmittance ......................................................................................108
6.7.1 Time course setup parameters...............................................................................................108
6.7.2 Time course scan reading ......................................................................................................109
6.7.3 Analysis of time course data ..................................................................................................110
6.7.3.1 Navigation of a time scan or a time scan analysis.........................................................110
6
Table of Contents
6.8 System Checks............................................................................................................................... 111

6.8.1 Instrument Information ........................................................................................................... 111
6.8.2 Update the instrument software ............................................................................................. 111
6.8.3 Optical Checks....................................................................................................................... 112
6.8.3.1 Verification kit................................................................................................................ 112
6.8.3.2 Wavelength check......................................................................................................... 114
6.8.3.3 Stray light check............................................................................................................ 114
6.8.3.4 Absorbance check ........................................................................................................ 115
6.8.4 AQA - Analytical Quality Assurance....................................................................................... 116
6.8.4.1 AQA Standard Configuration ........................................................................................ 116
6.8.4.2 AQA Addition ................................................................................................................ 118
6.8.4.3 Multiple Determination .................................................................................................. 118
6.8.4.4 Dilution .......................................................................................................................... 119
6.8.4.5 Carring out AQA measures ........................................................................................... 119
6.8.4.6 Pipette test set .............................................................................................................. 120
6.8.5 Instrument backup ................................................................................................................. 120
6.8.6 Service menu ......................................................................................................................... 121
6.8.7 Service time ........................................................................................................................... 121
6.8.8 Lamps operating time ............................................................................................................ 122
6.9 Toolbar............................................................................................................................................ 122
6.9.1 Log on .................................................................................................................................... 122
6.9.2 Sample ID .............................................................................................................................. 123
6.9.3 Timer...................................................................................................................................... 123
6.9.4 AQA ....................................................................................................................................... 123
6.9.5 Trends.................................................................................................................................... 124
6.9.6 Link2SC ................................................................................................................................. 124
6.9.7 Website DR 3900................................................................................................................... 124
Section 7 Maintenance ....................................................................................................................... 125
7.1 Cleaning requirements.................................................................................................................... 125
7.1.1 Spectrophotometer ................................................................................................................ 125
7.1.2 Display ................................................................................................................................... 125
7.1.3 Cuvettes/cells......................................................................................................................... 126
7.2 Lamp replacement .......................................................................................................................... 126
7.3 Exchange of the replaceable cell compartment.............................................................................. 129
Section 8 Troubleshooting................................................................................................................. 131
Section 9 Replacement Parts............................................................................................................. 135
9.1 Accessories .................................................................................................................................... 135
9.2 Replacement Parts ......................................................................................................................... 135
Section 10 Contact ............................................................................................................................. 137
Section 11 Limited warranty .............................................................................................................. 139
7
Table of Contents
Appendix A Help Guide ......................................................................................................................141

A.1 Display of the Help Guide for stored programs..............................................................................141
A.2 Display of the Help Guide for barcode programs..........................................................................142
Index .....................................................................................................................................................145
8
Section 1 Specifications
These are subject to change without notice!
Performance specifications
Operating mode Transmittance (%), Absorbance and Concentration
Source lamp Halogen lamp
Wavelength range 320–1100 nm
Wavelength Accuracy ± 1.5 nm (wavelength range 340–900 nm)
Wavelength reproducibility ± 0.1 nm
Wavelength resolution 1 nm
Wavelength calibration Automatic
Wavelength selection Automatic, based on method selection
Scanning speed  8 nm/s (in steps of 1 nm)
Spectral bandwidth 5 nm
Photometric measuring range ± 3.0 Abs (wavelength range 340–900 nm)

5 m abs at 0.0–0.5 abs
Photometric accuracy
1% at 0.50–2.0 abs
< 0.5% to 2 Abs
Photometric linearity
1% at > 2 Abs with neutral glass at 546 nm
Stray light < 0.1% T at 340 nm with NaNO2
Data log 2000 readings (Result, Date, Time, Sample ID, User ID)
User programs 100
Physical and environmental specifications
Width 350 mm (13.78 in)
Height 151 mm (5.94 in)
Depth 255 mm (10.04 in)
Mass 4200 g (9.26 lb)

10–40 °C (50–104 °F), maximum 80% relative humidity (without condensate
Ambient operating requirements
formation)
–40–60 °C (–40–140 °F), maximum 80% relative humidity (without condensate
Ambient storage requirements
formation)
Additional technical data
Power connector via external power Input:100–240 V/50–60 Hz

supply Output: 15 V/30 VA
Use only shielded cable with maximum length of 3 m:
2x USB type A
Interfaces 1x USB type B
Use only shielded cable (e.g. STP, FTP, S/FTP) with maximum length of 20 m:
1x Ethernet
9
Specifications
Performance specifications
Enclosure rating IP40 (excluding interfaces and power supply)
Protection class Class I
This product has been tested to the requirements of CAN/CSA-C22.2

No. 61010-1, second edition, including Amendment 1, or a later version of the
same standard that incorporates the same level of testing requirements.
10
Section 2 General Information
2.1 Safety information

Please read this entire manual before unpacking, setting up, or
operating this equipment. Pay attention to all danger and caution
statements. Failure to do so could result in serious injury to the
operator or damage to the equipment.
To make sure that the protection provided by this instrument is
not impaired, do not use or install this instrument in any manner
other than that specified in these operating instructions.
DANGER
Indicates a potentially or imminently hazardous situation that, if not
avoided, results in death or serious injury.
WA R N I N G
Indicates a potentially or imminently hazardous situation that, if not
avoided, may result in death or serious injury.
CAUTION
Indicates a potentially hazardous situation that may result in minor or
moderate injury.
NOTICE
Indicates a situation that, if not avoided, could result in damage to the
instrument. Information that requires special emphasis.
Note: Information that supplements points in the main text.
2.1.1 Precautionary labels

Read all labels and tags attached to the instrument. Personal
injury or damage to the instrument could occur if this information
is not observed. A symbol, if noted on the instrument, will be
included with a danger or caution statement in the operating
instructions.
This symbol, if noted on the instrument, references the user manual for operation and/or safety information.
Electrical equipment marked with this symbol may not be disposed of in European domestic or public disposal
systems after 12 August 2005. In conformity with European local and national regulations (EU Directive
2002/96/EC), European electrical equipment users must now return old or end-of life equipment to the
manufacturer for disposal at no charge to the user.
Note: For return for recycling, please contact the equipment manufacturer or supplier for instructions on how to
return end-of-life equipment, manufacturer-supplied electrical accessories, and all auxiliary items for correct
disposal.
11
General Information
2.1.2 RFID module (not available on all models)

RFID technology is a radio application. Radio applications are
subject to national conditions of authorisation. The use of the
DR 3900 (model with RFID module) is currently permitted in the
following countries: EU, CH, NO, HR, RS, MK, TR, CY, US, CA,
AU, NZ. The manufacturer advises that the use of the DR 3900
(model with RFID module) outside of the above-mentioned
regions may contravene national laws. The manufacturer
reserves the right also to obtain authorisation in other countries.
In case of doubt, please contact the distributor.
The DR 3900 contains an RFID module to receive and transmit
information and data. The RFID module operates with a
frequency of 13.56 MHz.
WA R N I N G
The spectrophotometer may not be used in dangerous environments.
The manufacturer and its suppliers reject any express or indirect
guarantee for the use with High Risk Activities.
Follow the following safety information, in addition to any local

guidelines in force.
Safety information for the correct use of the instrument:
• Do not operate the instrument in hospitals or comparable
establishments in proximity to medical equipment, like pace
makers or hearing aids.
• Do not operate the instrument near highly flammable
substances, such as fuels, highly combustible chemicals and
explosives.
• Do not operate the instrument near combustible gases,
vapors or dust.
• Do not vibrate or jolt the instrument.
• The instrument can cause interference in immediate
proximity to televisions, radios and computers.
• Do not open the instrument.
• Improper use of the instrument voids the warrantee.
2.1.3 Chemical and Biological Safety
DANGER
Potential danger in the event of contact with chemical/biological
materials.
Handling chemical samples, standards and reagents can be
dangerous. 
Familiarise yourself with the necessary safety procedures and the
correct handling of the chemicals before the work and read and follow
all relevant safety data sheets.
Normal operation of this instrument may involve the use of

hazardous chemicals or biologically harmful samples.
12
General Information
• Observe all cautionary information printed on the original

solution containers and safety data sheet prior to their use.
• Dispose of all consumed solutions in accordance with
national regulations and laws.
• Select the type of protective equipment suitable to the
concentration and quantity of the dangerous material at the
respective work place.
2.2 Overview of product

The DR 3900 is a VIS spectrophotometer with a wavelength
range of 320 to 1100 nm. The instrument is delivered with a
complete series of application programs and supports several
languages.
The spectrophotometer DR 3900 contains the following programs
and operating types:
• Stored programs (pre-installed tests)
• Barcode Programs
• User Programs
• Favorites
• Single Wavelength
• Multi Wavelength
• Wavelength Scan
• Time course
The DR 3900 Spectrophotometer provides digital readouts in
direct concentration units, absorbance or percent transmittance.
When selecting a user-generated or programmed method, the
menus and prompts serve to direct the user through the test.
This menu system can also generate reports, statistical
evaluations of generated calibration curves and to report
instrument diagnostic checks.
13
General Information
14
Section 3 Installation
WA R N I N G
Electrical and fire hazards. 
Only use the supplied benchtop power supply LZV844.
Only qualified experts may perform the tasks described in this section
of the manual, while adhering to all locally valid safety regulations.
3.1 Unpack the instrument

The DR 3900 Spectrophotometer comes packaged with the
following items:
• DR 3900 spectrophotometer
• Dust Cover
• USB dust cover, fitted as standard
• Benchtop power supply with power cord for US, GB
• Cell adapter A
• Light shield, fitted as standard in the DR 3900
• Operator RFID tag (not available on all models)
• Basic operating instructions DR 3900, operating instructions
LINK2SC
• CD-ROM with HACH and HACH-LANGE procedural
instructions and extensive operating instructions
Note: If any of these items are missing or damaged, please contact the
manufacturer or a sales representative immediately.
3.2 Operating environment

Note the following points so that the instrument will operate
perfectly and have a long service life.
• Place the instrument firmly on an even surface. Do not push
any objects under the instrument.
• The ambient temperature must be 10–40 °C (50–104 °F).
• The relative humidity should be less than 80%; moisture
should not condense on the instrument.
• Leave at least a 15 cm clearance at the top and on all sides
for air circulation, to avoid overheating of electrical parts.
• Do not operate or store the instrument in extremely dusty,
humid or wet locations.
• Keep the surface of the instrument, the cell compartment and
all accessories clean and dry at all times. Immediately
remove splashes or spilt materials on or in the instrument
(refer to Section 7).
NOTICE
Protect the instrument from extreme temperatures from heaters,
direct sunlight and other heat sources.
15
Installation
3.3 Front and back view

Figure 1 Front view
1 USB port type A 4 On/off switch

2 Cell compartment cover 5 RFID module (not available on all models)
3 Touch screen
16
Installation
Figure 2 Back view
1 Ethernet port 3 USB port type A

2 USB port type B 4 Connection for benchtop power supply
3.4 Power connections
WA R N I N G
Electrical and fire hazards. 
Only use the supplied benchtop power supply LZV844.
1. Connect the power cable to the benchtop power supply.

2. Plug the benchtop power supply cable into the back of the
instrument (Figure 2, page 17).
3. Insert the power cable plug into a mains socket
(100–240 V~/50–60 Hz).
4. Turn on the power button next to the display to turn on the
power supply (Figure 1, page 16).
17
Installation
3.5 Interfaces
The DR 3900 features three USB ports and one Ethernet port as
standard, located on the front and rear sides of the instrument
(Figure 1 and Figure 2, page 17).
The USB type A ports are used for communications with a printer,
USB memory stick or keyboard. A USB memory stick can be
used to update the instrument software.
The USB type B port is used for communications with a PC. The
optional Hach Data Trans software (refer to Section 9, page 135)
must be installed on the PC for data transfer.
A USB hub may be used to connect several accessories at a
time.
Note: USB cables must not be longer than 3 m.
These USB ports enable data and graphics to be output to a

printer or PC and they enable instrument software to be upgraded
(refer to Section 6.8.2, Page 111). The Ethernet port supports
real-time data transfer in local networks, LIMS systems or SC
controllers. Only use a shielded cable (e.g. STP, FTP, S/FTP)
with a maximum length of 20 m for the Ethernet port.
3.6 Cell compartments, cell adapters and light shield

3.6.1 Cell compartments and adapters
The DR 3900 has two cell compartments (Figure 3). Only one cell
type at a time can be used for a reading.
Cell compartment (1) for:

• 13-mm and 16-mm round cells
Note: Cell compartment (1) contains barcode detection for 
cells/vials.
Cell compartment (2) for:

The following cell types can be used in cell compartment (2).
• Without cell adapter A, 50-mm cells, 1-inch rectangular cells
and 1-inch flow cells can be used directly in cell compartment
(2).
• With cell adapter A: 10 mm rectangular cells, 1-inch round
cells and AccuVac® vials.
Note: These cells must be inserted using cell adapter A.
18
Installation
Figure 3 Cell compartments
1 Cell compartment for round cells (1) 2 Cell compartment for rectangular cells (2)
Figure 4 Cell adapter
Cell adapter A: 10-mm rectangular cells/1-inch round cell
19
Installation
3.6.2 Installation of cell adapter

1. Open the cell compartment.
2. Place the cell adapter into cell compartment (2) so that the
arrow on the cell adapter points to the rear (Figure 5).
Note: The arrow on top of the cell adapter indicates the direction of
the light beam path.
Figure 5 Installation of a cell adapter
20
Installation
3.6.3 Use of the light shield for measurements

The light shield (Figure 6) prevents disruptive exposure to light
when 13-mm or 16-mm round cells are used in the cell
compartment (1).
Insert the light shield into cell compartment (2) before performing
measurements in cell compartment (1).
The DR 3900 is shipped with the light shield installed. Remove
the light shield before using cell compartment (2).
Figure 6 Light shield
Installation of the light shield

1. Open the cell compartment.
2. Place the light shield into cell compartment (2) so that the
arrow on the light shield points to the rear.
3.7 Beam path

Figure 7 shows the beam path of the DR 3900.
21
Installation
Figure 7 Beam path
1 Halogen lamp 8 Splitter mirror

2 Entrance slit 9 Reference element
3 Grating 10 Cell compartment (2) for rectangular cells
4 Grating angle indicator 11 Lens
5 Exit slit 12 Reading element
6 Lens 13 Cell compartment (1) for round cells
7 Filter wheel
22
Section 4 Startup
4.1 Switch on the instrument, startup process

1. Connect the power cable to the mains outlet.
2. Switch on the instrument by using the power button next to
the display.
3. The instrument starts automatically with a startup process
lasting approximately 45 seconds. The display shows the
logo of the manufacturer. At the end of the startup process, a
startup melody is heard.
Note: Wait approximately 20 seconds before switching on again so as
not to damage the electronics and mechanics of the instrument.
4.2 Language selection
The DR 3900 software includes several language options. The

first time the instrument is switched on, the language selection
screen will be shown automatically after the startup process.
1. Select the required language.
2. Press OK to confirm the language selection. The self-check
will then start automatically.
Change the language setting

The instrument functions in the selected language until the option
is changed.
1. Turn the instrument on.
2. During the startup process, touch any point on the screen
and maintain contact until the option for selecting a language
is shown (approximately 45 seconds).
3. Select the required language.
4. Press OK to confirm the language selection. The self-check
will then start automatically.
4.3 Self-check
Each time the instrument is powered up, a test program begins.

This procedure, which takes approximately two minutes, checks
the system, lamp, filter adjustment, wavelength calibration and
voltage. Each test that functions correctly is marked accordingly.
The Main Menu is displayed when diagnostics are completed.
Note: In the event of further error messages during the test program,
refer to Section 8, page 131.
23
Startup
4.4 Sleep mode
The instrument can be put into sleep mode.

1. Briefly press the power button next to the display.
The "Sleep mode" message is shown. The display will then
switch off automatically.
2. To switch on, press the power button next to the display.
The self-check will start automatically. 
After that, the instrument is ready to use.
4.5 Power off the instrument

1. Press the power button next to the display for approximately
5 seconds.
24
Section 5 Standard programs
5.1 Overview
5.1.1 Tips for the use of the touch screen
The whole screen responds to touch. To choose an option, tap
with a fingernail, fingertip, an eraser or a specialised stylus. Do
not press the screen with a sharp object, such as the tip of a
ballpoint pen.
• Do not place anything on top of the screen, to prevent
damage or scratches on the screen.
• Press buttons, words or icons to select them.
• Use scroll bars to move up and down long lists very quickly.
Press and hold the scroll bar, then move up or down to move
through the list.
• Highlight an item from a list by pressing it once. When the
item has been successfully selected, it will be displayed as
reversed text (light text on a dark background).
5.1.2 Use of the alphanumeric keypad
This display is used to enter letters, numbers and symbols as

needed when programming the instrument. Unavailable options
are disabled (grayed out). The icons on the right and left of the
screen are described in Table 1.
The central keypad changes to reflect the chosen entry mode.
Press a key repeatedly until the desired character appears on the
screen. A space can be entered by using the underscore on the
YZ_ key.
Press Cancel to cancel an entry, or press OK to confirm an entry.
Note: It is also possible to use a USB keyboard (with US keyboard
layout) or a hand-held USB barcode scanner (refer to Section 9,
page 135).
Table 1 Alphanumeric keypad

Icon / key Description Function
ABC/abc Alphabetic Toggles the character input mode between upper and lower case.
#% Symbols Punctuation, symbols and numerical sub- and superscripts may be entered.
123 Numeric For entering regular numbers.
CE Clear Entry Clear the entry.
Left Arrow Back Deletes the current character and goes back one position.
Right Arrow Next Navigates to the next space in an entry.
25
Standard programs
5.1.3 Main menu
A variety of modes may be selected from the Main Menu. The

following table briefly describes each menu option.
There is a toolbar on the right-hand side of the screen. Press to
activate the various functions.
Table 2 Main Menu options

Option Function
Stored programs are pre-programmed methods that make use of HACH chemicals and
Stored Programs / HACH-LANGE pipette tests.
Barcode Programs The DR 3900 Procedures Manual contains illustrated, step-by-step procedures for analyses
(HACH-LANGE programs) using HACH programs. The working procedures for HACH-LANGE tests are included in the
test packs.
User programs make "made to measure analysis" possible:
• Users can program methods they have developed themselves
User Programs
• Existing HACH and LANGE methods can be stored as user programs. The
HACH-LANGE tests can then be modified to suit the user's requirements.
Favorites List of methods/tests created by the user to suit his own requirements.
Single wavelength readings are:
Absorbance readings: The light absorbed by the sample is measured in absorbance units.
Single Wavelength Transmittance reading (%): Measures the percent of the original light that passes through the
sample and reaches the detector.
Concentration readings: A concentration factor can be entered to enable the measured
absorbance values to be converted into concentration values.
In the Multi Wavelength mode, absorbance (Abs) or percentage transmittance (%T) is
Multi Wavelength measured at up to four wavelengths and absorbance differences and absorbance relationships
are calculated. Simple conversions into concentrations can also be carried out.
A wavelength scan shows how the light from a sample is absorbed over a defined wavelength
spectrum. This function can be used to determine the wavelength at which the maximum
Wavelength Scan
absorbance value can be measured. The absorbance behavior is displayed graphically during
the scan.
The time scan records the absorbance or % transmittance at a wavelength over a defined
Time course
time.
The "System check" menu offers a number of options, including optical checks, output checks,
System checks lamp history, instrument update, service time, settings for analytical quality assurance and
instrument backup.
Recall measurement data Stored data can be called up, filtered, sent and deleted.
In this mode, user-specific or method-specific settings can be entered: operator ID, sample ID,
Instrument Setup
date & time, sound, PC & printer, password, energy-saving mode and stored data.
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Standard programs
5.2 Instrument Setup
1. Select Instrument Setup in the Main Menu.

A selection of functions appears in order to configure the
functions of the instrument.
5.2.1 Operator ID
Use this option to enter up to 30 sets of operator initials (up to ten
characters each) into the instrument. This feature helps record
which operator measured each sample.
1. Press Operator ID in "Instrument Setup".

2. Press Options>New to enter a new operator ID.
Note: If no operator ID has yet been entered, the alphanumeric
keyboard is immediately displayed.
3. Use the alphanumeric keypad to enter a new operator ID.

4. Press OK to confirm the entry.
5. Choose an icon for the operator ID using the Left Arrow and
Right Arrow.
6. Press Operator Password to protect the operator ID with a
password.
7. Use the alphanumeric keyboard to enter an operator
password.
9. Press Cancel to delete the complete entry for the operator

ID. 
Press Back to reach to the input screen for operator ID. 
Press OK to confirm the entry. The following screen is
displayed.
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Standard programs
10. Press Back to return the instrument to the "Instrument

Setup" menu.
Press Logout to log out of an active operator ID.
Press Login to activate a selected operator ID.
Press Options to enter, change or delete additional operator
IDs. The following screen is displayed.
11. Press New to enter another operator ID.

Press Delete to delete an operator ID.
Press Edit to edit an operator ID.
Press Initialize RFID Tag to write the operator ID to an
optional operator RFID tag (not available on all models).
Refer to Section 5.2.1.1 for more information.
5.2.1.1 Operator RFID tag (not available on all models)
1. Define an operator ID (refer to Section 5.2.1).

2. Press Initialize RFID Tag.
3. Follow the instructions on screen and hold the operator RFID

tag over to the front-right of the RFID module.
A sound indicates that the write process has been sucessful.
4. The operator RFID tag can be newly written at any time. To
do so, confirm the "Overwrite Operator ID" message with OK
and enter the password if the old operator ID is protected
with a password.
If the operator RFID tag is successfully overwritten, a
message confirming this will briefly be shown on the display.
The operator is logged on and the operator ID is shown in the
toolbar.
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Standard programs
5.2.2 Sample ID
Use this option to enter up to 100 Sample Identification tags (up
to 20 characters each) into the instrument. Sample IDs can be
used to specify the sample location or other sample specific
information.
1. Press Sample ID in the toolbar on the right.

2. Press New to enter a new sample ID.
Note: If no sample ID has been entered yet, the alphanumeric
keyboard is immediately displayed.
3. Use the alphanumeric keypad to enter a new Sample ID.

Note: If a USB Barcode handset scanner ( refer to Section 5.2.2.1,
page 30) is connected, Sample IDs can also be scanned.
5. Assign the current time and date, a sequential number or a

colour to the sample ID.
6. To number the sample IDs sequentially—e.g. Inflow (01)

etc.—press Add Number.
• Use the arrow keys to specify the first number of the
sequence.
• Use the key between the arrow keys to enter the first
number of the sequence using the alphanumeric keypad.
7. Press OK to return to the "Instrument Setup" menu.
The Sample ID is activated. Each Sample ID is automatically
numbered in ascending order after a reading. The number is
shown in parentheses behind the Sample ID.
8. Press Back to return the instrument to the "Instrument

Setup" menu.
Press Off to switch off the active sample ID.
Press Select to activate the selected sample ID.
Use Options to enter, change or delete additional sample
IDs. The following screen is displayed:
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Standard programs
9. Press New to enter another sample ID.

Press Delete to delete a sample ID.
Press Edit to edit a sample ID.
Press Initialize RFID Tag to write the sample ID to an
optional location RFID tag (not available on all models).
5.2.2.1 Sample ID with scanner method 1

1. Connect the scanner to the USB port.
Successful connection is indicated by a sound.
2. Press Sample ID>Options and New.
3. Read barcode with scanner.
4. The sample ID can have the current date and time, a

sequential number and a colour allocated to it.
Select the required options or a colour.
6. Press New again and repeat the process for each barcode.
5.2.2.2 Sample ID with scanner method 2

1. Read the sample ID in the measurement window with the
barcode scanner and measure the cell.
The barcode is stored along with the reading, but is not
adopted into the list of sample IDs.
Note: To delete a sample ID, select the ID and press Delete.
Note: A sample ID can be entered or changed in Reading Mode. On the

results screen, press Options>More>Instrument Setup. If a Sample ID
is already assigned, select the "Sample ID" symbol in the result display.
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Standard programs
5.2.3 Sample ID and operator ID with the optional RFID Sample ID Kit (not available on
all models)
The optional RFID Sample ID Kit consists of:
• A LOC 100 RFID Locator
• An operator RFID tag
• Five location RFID tags and
• Three sample bottle RFID labels each, in black, red, green,
blue and yellow
The operator and location RFID tags can be written to and read in
by the RFID module in the DR 3900 as often as required.
More information can be found in the user manual for the
LOC 100 RFID Locator.
5.2.4 Security settings

The "Security" menu contains a variety of security settings to
control access to various functions.
All functions can be allocated to three different security levels:
• not secured: every operator can make changes in this area.
• one key: every operator assigned this security level can
make changes to non-secured functions and functions with
one key.
• two keys: every operator assigned this security level can
make changes in all functions
1. Press Security in the "Instrument Setup" menu.

2. In order to open the Security List, assign a password as
security administrator. Press Security Password.
3. Use the alphanumeric keypad to enter a new Password (up

to 10 characters each) and press OK to confirm.
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Standard programs
4. Press Security List to use various functions with security

level 1 or 2.
5. Select the required function and press Setup.
6. Select the required security level (two keys, one keys or off)
and confirm using OK.
7. Confirm the Security List using OK to return to the
"Security" menu.
8. Press On to activate the new settings of the security list.

9. Press OK to return to the "Instrument Setup" menu.
Note: The alphanumeric keypad for entering the password appears
when a user tries to reach a locked setting.
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Standard programs
5.2.4.1 Assign operator security level

Every operator with an operator ID can be assigned a security
level. This is connected to the operator password. Setup must be
coordinated with the security administrator and the operator.
1. Press Operator ID in the "Instrument Setup".

2. Enter the security password and confirm by pressing OK.
3. Set up an operator ID (refer to Section 5.2.1).
4. Press Operator Password.

5. Enter the operator password and confirm with OK.
6. Press Security Level <Off>

7. Enter the security password and confirm with OK.
The current security level for the selected operator is shown.
8. Select the required security level for this operator ID and

confirm with OK.
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Standard programs
9. Press OK.
The operator ID is displayed with the selected security level.

10. Activate the selected operator ID by pressing Login.
11. Enter the operator password.
12. Confirm with OK and return to "Instrument Setup".
5.2.4.2 Deactivate password
1. Press Security in the "Instrument Setup" menu.

2. Enter the security password and confirm with OK.
3. Press Off to deactivate the settings of the Security List.

4. Press OK and return to the "Instrument Setup" menu.
Note: Use this function to delete the former password or to enter a new
one.
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Standard programs
5.2.5 Date and time
1. Press Date & Time in the "Instrument Setup".
2. Select a Date Format for the date.

4. Select a Time Format for the time.

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Standard programs
7. Enter the current date and time. Change the information

using the arrow keys.
The instrument will return to "Instrument Setup".
5.2.6 Sound settings
1. Press Sound Settings in "Instrument Setup".

The following options will be displayed:
• All: Activates/deactivates a sound with a variable volume for
every function, with the exception of the timer.
• Touch screen: Activates/deactivates a short sound with
variable volume every time the screen is touched.
• Reading done: Activates/deactivates a short sound with
variable volume when the reading is done.
• Timer: Sets short/long sound with variable volume when
timer has ended.
• Startup: Activates/deactivates a startup melody with variable
volume when the test program is started.
• Warning: Activates/deactivates a short sound with variable
volume if there is an error message.
• RFID done: Activates/deactivates a short sound with variable
volume when a RFID transfer is done.
• Shutdown: Activates/deactivates a short sound with variable
volume when shutting down.
2. Select the required action.

3. Press Setup and complete the required settings.
The instrument then returns to "Instrument Setup".
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Standard programs
5.2.7 PC and printer

The DR 3900 features an Ethernet port and two USB ports on the
rear of the instrument, as well as one USB port on the front (refer
to Figure 1 and Figure 2, page 17). These interfaces can be used
for exporting data and graphics to a printer, updating data and
transferring data to a PC and in a network. These interfaces can
be used for the connection of a USB stick, an external USB
keyboard or a USB Barcode handset scanner.
Note: Various accessories can also be connected simultaneously using
a USB hub.
A USB memory stick is used to update data, refer to

Section 6.8.2, page 111.
Note: A shielded USB cable must not be longer than 3 m!
Table 3 USB connector

Interfaces Description
USB (Type A) This USB port can be used to connect a printer, a USB memory stick or a keyboard.
This USB port is only intended for the connection of the DR 3900 to a PC (with installation of the
USB (Type B)
corresponding software).
The Ethernet port is intended for data transfer to a PC without installed software or in a local
Ethernet network . Only use a shielded cable (e.g. STP, FTP, S/FTP) with a maximum length of 20 m for
the Ethernet port.
1. Press PC & Printer in "Instrument Setup".

A list with information about the connections opens.
5.2.7.1 Printer setup
2. Press Printer.
3. Press Setup to display the Printer Setup screen.
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Standard programs
Printer Setup:
• Resolution: Font size
• Paper: Paper size
Note: If an optional Thermal Printer is connected, the function "Auto
Send" on/off is available.
4. Press Resolution to select the print quality.

Select between:
• 100 dpi,
• 150 dpi and
• 300 dpi.
Note: Press OK again to return to the "Instrument Setup" menu.
6. Press Paper to select the paper size.

Select between:
• Letter,
• Legal,
• Executive,
• A4.
Note: Press OK again to return to the "Instrument Setup" menu.
5.2.7.2 Print data
1. Press Recall Data from the Main Menu.

2. Select the data source, where the data to be printed are
stored.
A list is displayed. Data can be filtered. Refer to
Section 5.3.1.2, page 44 for more information.
3. Press the Printer icon to send the data (table, curve)
immediately to the printer.
4. Highlight Single point, Filtered data or All data and press
OK to confirm.
Send data... appears on the display until the data is printed.
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Standard programs
5.2.7.3 Print data continuously

If all readings are to be printed out automatically straight after
measurement, the use of a continuous form printer is
recommended, e.g. the 4" paper thermal printer. Refer to
Section 9, page 135.
1. Connect the thermal printer to a USB type A port.
2. Press PC & Printer in the "Instrument Setup" menu.
A list with information about the connections opens.
3. Highlight Printer.
4. Press Setup to display the Printer Setup screen.
5. Select Auto-Send: On to send all measured data
automatically to the Thermal printer.
5.2.7.4 Network setup

The DR 3900 supports communication in local networks. To
establish Ethernet communication in networks, special
configuration is required. The manufacturer recommends support
from a network administrator. Only use a shielded cable
(e.g. STP, FTP, S/FTP) with a maximum length of 20 m for the
Ethernet port. This section describes the setting options for the
photometer in relation to network communication.
The connection parameters must be configured for data transfer

to a PC or connection to a network.
1. Press PC & Printer in "Instrument Setup".
2. Press Network>Setup.
3. Press On.
4. Press IP address.
An IP address is used to send data from a sender to a
pre-defined recipient.
• If the IP for the DR 3900 is automatically issued in the
network, no further settings are necessary. A hostname
can optionally be assigned.
• If the instrument address is fixed, the connection must be
configured.
39
Standard programs
5. Select Fix to configure the IP address.
6. Press IP address and enter the address.
In conjunction with the IP address of an instrument, the subnet

mask establishes which IP addresses are located within the local
network.
7. Press Subnet Mask and enter the address.
A Gateway enables data communication in networks that are

based on varying protocols.
8. Press Default Gateway and enter the address.
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Standard programs
10. Press Network Server: <Off> to determine the target site for
the data transfer.
11. Select Netdrive and run Netdrive Setup.

Alternatively, select FTP and run FTP Setup.
The procedures are completely identical. As an example,
Netdrive Setup is described here.
The target site can be defined by IP address or a server name.

12. Press IP-Address and enter the IP address or select Server
Name and enter the server name.
13. Press Data Folder and enter the target folder name.
Note: A folder with the selected name must exist on the target
system. Additionally, the folder must be shared with the operator,
using the corresponding password.
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Standard programs
User and password must be identical to the settings on the

target system.
15. Press User and enter the username.
16. Press OK to confirm.
17. Press Password and enter the password.
19. Press Factory Default to restore the Network Settings to the

factory defaults.
5.2.8 Power Management
1. Press Power Management in "Instrument Setup".

2. Select Sleep Timer and press OK.
3. Select the required time span after which the instrument will
go into energy-saving sleep mode if it is not used (refer to
Section 4.4, page 24), and confirm with OK.
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Standard programs
4. Select Power Off Timer and press OK.

5. Select the required time span after which the instrument will
automatically be powered off if it is not used, and confirm
with OK.
The instrument then returns to "Instrument Setup".
5.3 Save, recall, send and delete data

5.3.1 The data log
The data log can store up to 2000 readings saved by the
following programs:
• Stored Programs,
• Barcode Programs,
• User programs,
• Favorites,
• Single Wavelength and
• Multi Wavelength.
A complete record of the analysis is stored, including the Date,
Time, Results, Sample ID and Operator ID.
5.3.1.1 Auto/manual data storage

The data storage parameter indicates whether data is to be
stored automatically or manually (in which case the user has to
decide which data to store).
1. Press Data Log Setup in the "Instrument Setup" menu.

• When the setting Auto Store: On is activated, the
instrument will store all measurement data automatically.
• When the setting Auto Store: Off is activated, the
instrument will not store any measurement data.
However, this setting can be changed on the results
screen, via Options>Auto Store: On. The reading
currently shown in the display is then stored.
2. Specify the output format of data to be exported using Send
Data Format. Select xml or csv.
The instrument will return to "Instrument Setup".
Note: When the instrument memory (data log) is full, the oldest data is
automatically deleted allowing the new data to be stored.
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Standard programs
5.3.1.2 Recall stored data from the data log
1. Press Recall Data in the Main Menu.
2. Press Data Log.

A listing of the stored data is displayed.
3. Press Filter: On/Off. 

The function Filter Settings is used to search for specific
items.
4. Activate On. The data can now be filtered using the following
selection criteria.
• Sample ID
• Operator ID
• Start Date
• Parameter
or any combination of the four.
5. Press OK to confirm the selection.

The chosen items are listed.
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Standard programs
6. Press View Details to get more information.
5.3.1.3 Send data from the data log

Data is sent from the internal data log as an XML (Extensible
Markup Language) file or as a CSV (Comma Separated Value)
file, to a directory with the name DataLog on a USB mass storage
device or a network drive. The file can then be processed using a
spreadsheet program. The file name has the format:
DLYear-Month-Day_Hour_Minute_Second.csv or
DLYear-Month-Day_Hour_Minute_Second.xml.
To send data to a printer, refer to Section 5.2.7.2, page 38.
1. Plug the USB storage device into the USB type A port on the
DR 3900, or connect the DR 3900 to a network drive (refer to
Section 5.2.7.4, page 39).
3. Select the data category to be transferred, e.g. Data Log.
A list of the selected measurement data is displayed.
4. Press Options and then the PC & Printer icon.
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Standard programs
5. Select the data to be sent.

The following options are available:
• Single point: only this selected reading will be sent
• Filtered data: only readings that correspond to the set
filters will be sent
• All data: all data in the selected data category will be
sent.
Note: The number in parentheses is the total number of data sets
assigned to this selection.
5.3.1.4 Delete stored data from the data log

2. Press Data Log>Options>Delete.
3. Highlight Single point, Filtered data or All data and press
OK to confirm.
Note: The number in parentheses is the total number of data sets
assigned to this selection.
5.3.2 Control charts for data from the AQA Log
1. Press Recall Data in the Main Menu.
2. Press AQA Log.

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Standard programs
3. Press Filter: On/Off.

4. Activate On.
5. Select a required parameter.

The chosen items are listed.
7. Press Options>Control Chart.
8. Press Standard Control Chart to choose the control chart

of the selected standard.
The measured standard concentrations are displayed

graphically with the control limits and date.
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Standard programs
9. Press Range Control Chart to select the control chart of the

range in % for multiple determinations.
The range in % is displayed graphically with the control limits

and date.
5.3.3 Store, recall, send and delete data from wavelength scan and time course
The instrument can store 20 datasets for the Wavelength Scan
and 20 datasets for the Time Course. The data must be stored
manually after it is displayed.
5.3.3.1 Data storage from wavelength scan or time course
1. Press the Folder icon in the "Options" menu after a reading

is taken.
The Store Data list will be displayed.

2. Press Store to save the current scan to the highlighted
numbered line.
Note: Scans can be overwritten.
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Standard programs
5.3.3.2 Recall stored data from wavelength scan or time course

a. Select Wavelength Scan or Time Course to recall data.
b. If a program is already running, press
Options>More>Recall Data.
2. Select the required data.
3. Press Graph to look at the details.

Note: Press View Summary to return to the "Recall Data" list.
4. Press Table to look at the details.

Note: Press View Summary to return to the "Recall Data" list.
5.3.3.3 Send data from wavelength scan or time course

There are two ways to recall sent data to a USB memory stick,
printer or PC with Hach Data Trans.
Option 1:
1. Press Recall Data in the Main Menu and then Wavelength
Scan or Time Course.
2. Press Options and then the PC & Printer icon to send the
data to a USB memory stick, to a printer or to a PC with
Hach Data Trans.
• When a printer is connected, select how to send the data
to the printer (graph, table or both graph and table).
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Standard programs
• If a USB memory stick is connected, the files will be

automatically sent to the USB memory stick as xml or csv
files in the file folder "WLData (data of a wavelength
scan) or "TCData" (data of a time course).
The file name will be formatted as: "ScanData_X.csv"
(Wavelength Scan Data) or "TCData_X.csv" (Time Course
Data).
X = number of scans (1–20).
For further processing use a spreadsheet program.
Note: The message, "Data already present. Overwrite?" is displayed if
the files are already stored. Press OK to overwrite the stored data.
Option 2:
1. Press Wavelength Scan or Time Course and then
Options>More>Send Data to send the data to a USB
memory stick or to a printer.
• When a printer is connected, select how to send the data
to the printer (graph, table or both graph and table).
• If a USB memory stick is connected, the files will be
automatically sent to the USB memory stick as xml or csv
files in the file folder "WLData (data of a wavelength
scan) or "TCData" (data of a time course).
The file name will be formatted as: "ScanData_X.csv"
(Wavelength Scan Data) or "TCData_X.csv" (Time Course
Data).
X = number of scans (1–20).
For further processing use a spreadsheet program.
5.3.3.4 Delete stored data from wavelength scan or time course
1. Press Recall Data in the Main Menu and then Wavelength

Scan or Time Course.
2. Highlight any data to delete.
3. Press Delete in the "Options" menu and press OK to
confirm.
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Standard programs
5.3.4 Data analysis

Consistent use of the sample ID function is necessary for all data
analysis functions. Only data with identical sample IDs can be
used in data analysis functions.
Use the Trends function to display a time-variation curve for the
stored readings for each parameter and location. The
concentration of the the corresponding parameter is shown
graphically over time.
Use the Ratio function to monitor the parameter relationships at a
particular location and display them in a graph.
5.3.4.1 Trends
1. Press Recall Data>Data analysis.
2. Choose Trends and press OK to confirm.

Alternatively, choose Trends from the toolbar.
3. Press Options>New.
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Standard programs
4. Select the required parameter and press Next to confirm.

5. Select the required sample ID and press Next to confirm.
6. Select the required operator ID and press Next to confirm.
7. Select the required data analysis interval or the start and end
date, and press Next to confirm.
8. Set an upper and lower control limit and press Next to

confirm.
9. All of the selected functions are dispalyed in Trend Settings.

Press OK to confirm.
10. Select the required trend with the created settings from the
list. Press Select.
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Standard programs
The trend can be displayed in several formats, as a line or a

point.
11. Select Information to see an overview of the parameter
information.
12. Select Options>Add Data to add more readings to the
trend.
13. Select Options>Ratio:Off to activate the Ratio function.
The ratio corresponds to the first row of readings selected.
5.3.4.2 Ratios
The Ratio function is based on the precise classification of
samples by their sample locations using the optional accessories
"LOC 100 RFID Locator" and "RFID Sample ID Kit". The precise
sample location, date and time are attached automatically. Ratio
analysis calculates a ratio between two or three parameters. The
last defined parameter is represented by 1. The first or second
parameters are calculated as a ratio against this, e.g. 4:2:1.
1. Select Recall Data>Data analysis.
2. Select Ratios.
If a ratio analysis has already been carried out, a list of the

defined analyses will be displayed.
3. Press Options.
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Standard programs
4. Press New, to define a new ratio analysis.
5. Select the required sample ID and press Next to confirm.
6. Select parameter I with the greatest concentration and press

Next to confirm.
7. Select parameter II with the middle concentration and press

Next to confirm.
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Standard programs
8. Select parameter III with the smallest concentration and

press Next to confirm.
9. Select the required data analysis interval.

It is possible to select a fixed interval and an specific
customised interval with start and end dates.
11. Choose an upper and lower control limit for the first and
second parameters and press Next to confirm.
12. All of the selected functions are displayed in Ratio Settings.

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Standard programs
13. Select the ratio with the defined settings from the ratio
directory. Press Select.
A table with the defined values is shown.
Values outside the defined limits are displayed in red.
5.4 Stored Programs

Over 200 pre-programmed processes can be recalled using the
Stored Programs menu. Stored Programs do not include
barcode tests.
5.4.1 Select a saved test/method; entering user-specific basic data
1. Press Stored Programs in the Main Menu to view an

alphabetical list of stored programs with program numbers.
The "Stored Programs" list will appear.
2. Highlight the required test.
Note: Select the program by name or scroll through the list using the
arrow keys. Highlight the program or press Select by No. to search
for a specific program number. Press OK to confirm.
3. Press Start to run the program. The respective

measurement window is displayed.
Note: All corresponding data (wavelength, factors and constants) is
already preset.
4. Follow the instructions for chemical procedures in the

relevant procedures manual. Procedure manual is on CD
with purchase of instrument.
Note: To display the procedural instructions in the display, press the
info icon. Not all programs have this option.
5.4.2 Stored programs options

1. Press Stored Programs in the Main Menu. Select the
required process and press Start.
2. Press Options enter specific data. The following describes
the options Table 4.
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Standard programs
Table 4 Stored programs options

Options Description
More For further Options

With the Store On setting, all measurement data is stored automatically. With the Store Off
Store Off/On
setting, no measurement data is stored.
% Trans/Conc/Abs To switch to % transmittance, concentration or absorbance readings
Send Data icon / To send data to a printer, network drive, computer or USB memory stick (USB type A or Ethernet
Send Data port)
This functions as a stopwatch. It helps to make sure that the steps of an analysis are correctly
timed (e. g. reaction times, wait times, etc., can be exactly specified). When the specified time has
Timer icon
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the reading
program.
A corrective dilution factor can be entered in order to take account of certain properties.
The number entered at the dilution factor prompt will be multiplied by the result to compensate for
Dilution Factor Off/On the adjustment. For example , if the sample has been diluted by a factor of 2, enter 2. The default
setting of the dilution factor is turned off.
Note: When a dilution is in effect, the dilution icon will appear on the display.
This option enables the accuracy of the measurements to be checked. The procedural instructions
Standard Addition
for test parameters contain a detailed explanation of how to use this function.
The procedural instructions for test parameters indicate whether a standard adjustment is
Standard Adjust
necessary and, if so, how to proceed.
Chemical Form Some of the stored tests/methods allow to select the chemical form and the measuring range.
Some of the stored tests/methods include the "Reagent Blank" function. This enables the reagent
blank value to be added to or subtracted from, the subsequent readings. The reagent blank value
Reagent Blank shifts the calibration curve along the y-axis, without changing the shape or gradient of the curve.
The effect corresponds to a y-axis intercept of the calibration straight line. This is clarified by the
following formula: concentration =[conc. factor) x Abs] - (reagent blank)
Save as User Program To store the selected parameters as a User Program, refer to Section 6.1, page 73.
Multiple determination This option allows the multiple determination of a sample.
Recall Data To recall saved measurement data or time courses, refer to Section 5.3, page 43.
Instrument Setup
Basic data for the instrument, refer to Section 5.2, page 27.
mode
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Standard programs
5.4.3 Use of program timers

Some procedures do not require the use of timers. Other
procedures require several timers. These timers are
pre-programmed into each Stored Program, along with a
description of the activity to be performed during the timed period.
1. Press the Timer icon.

2. Press OK to start the first timer.
The timer will count down on the screen.
3. To start the next timed activity for the Stored Program, press
the Timer icon and OK.
Note: Press Close to view the reading screen while the timer is
running. The time will be shown left side bottom instead of the date.
Note: Press Cancel to stop the timer midway through the

countdown.
Note: The timer will beep when the timed interval ends.
A general purpose timer is also available in many programs. Type

the timer icon and select General Timer. Enter the length of the
timed interval and press OK to start the timer. The timer will beep
when the timed interval ends.
5.4.4 Set the dilution factor

The Dilution Factor function is used to calculate the original
concentration of a sample that has been diluted by a known ratio.
1. Press Options>More…>Dilution Factor.

The number entered at the dilution factor prompt will be
multiplied by the result to compensate for the adjustment.
For example, if the sample has been diluted by a factor of 2,
enter 2. The default setting of the dilution factor is turned off.
2. Press OK to confirm. Press again OK.
58
Standard programs
Note: When a dilution is in effect, the dilution icon will appear on the
display.
Note: If using undiluted samples, set the dilution factor off again.
5.4.5 Run a standard adjust

The Standard Adjust functions allows the calibration curve for a
stored program to be adjusted based on analysis of a known
standard solution. The Accuracy Check section of written
procedures often suggests a standard solution concentration for
this purpose.
1. Follow the entire procedure, using a known standard for the

sample.
2. After reading the concentration, press
Options>More>Standard Adjust.
3. Press On.
The "Current Reading" will show the concentration. The box
on the right will show the default standard value for the test,
as mentioned in the procedure.
4. To set the standard concentration, press the button and enter
the new value. Press OK to confirm.
5. Press Adjust to enable the Standard Adjust. The Standard

Adjust icon will appear.
Note: The adjustment must be within certain limits, which vary with
each program. The allowable percentage is shown after
"Adjustment“.
5.4.6 Set the chemical form

Some Stored Programs allow a variety of chemical forms tobe
selected.
Press the unit (e. g. mg/L) or the chemical representation of the
evaluation form (e. g. Al3+). A list of available evaluation forms
appear. Select the required form by pressing the corresponding
entry in the list.
Note: To exit from the program, the evaluation form reverts to the
standard setting.
59
Standard programs
5.4.6.1 Change of the default setting of the chemical form
1. In the result display, press Options>More>Chemical Form.

2. A list of available evaluation forms appear. Select the new
default setting.
3. Press Save as Default.
The current result and all further readings will be displayed in the
new chemical form.
5.4.7 Run a reagent blank

Some of the stored tests/methods include the "Reagent Blank"
function. This enables the reagent blank value to be measured
and then taken into account in calculating the reading result.
Reading/analysis of a reagent blank:
1. Prepare the test corresponding to the
specification/procedural instructions. Use distilled water
instead of a sample to determine the reagent blank value.
2. Select the test. Insert the zero solution into the cell
compartment, if required by the procedural instructions.
Press Zero.
3. Insert the prepared sample cell into the cell compartment.
Press Read. The result will be displayed.
4. Press Options>More>Reagent Blank.

5. Press On to highlight the Reagent Blank function.
6. The concentration shown on the button is the reading of the
reagent blank. To use this value for more analyses of this
parameter, press OK.
7. To change the value displayed on the button, press the
button. Use the alphanumeric keypad to enter the new value.
8. Next press OK.
Note: The "Reagent Blank" function is deactivated when the reading
program is left. To use the same blank value later for other tests using
the same reagent lot, enter the value per step 7.
Note: The results calculated using the reagent blank value must lie
within the limits of the measuring range of the test/method.
60
Standard programs
Note: The reagent blank icon is shown in the result display (refer to
arrow) when the function is active.
5.4.8 To carry out a multiple determination

A sample can be measured several times using the multiple
determination functions.
1. Before reading the concentration, press

Options>More>Multiple Determination.
2. Press On.
The number of multiple determinations is shown under
"Measurements".
3. To change the value displayed on the button, press the
button. Use the alphanumeric keypad to enter the new value.
4. The Multiple Determination icon will be shown on the display.
5.4.9 Analysis of samples
1. Press Stored Programs and select a program.

Note: The procedural instructions are shown in the display by using
the info icon when available.
2. Insert the zero solution cell into the cell compartment.

3. Press Zero.
61
Standard programs
4. Remove the zero solution cell from the cell compartment.

Insert the sample cell into the cell compartment.
5. Press Read. The result will be displayed.
Note: To define a sample dilution, press the Dilution key in the
toolbar.
6. For data storage, refer to Section 5.3.1, page 43.
5.4.10 Update/edit tests (not available on all models)
5.4.10.1 General advices for program update and edit

For a manual test update and for a new programming of a test,
refer to Section 5.4.10.2, page 62 and Section 5.4.10.3, page 63.
Note: Only in very few cases does the revision of a test require the test
specifications to be updated.
To update and edit barcode tests, refer to Section 5.5.5, page 67.
5.4.10.2 Manual update of test data

When a test is selected the instrument automatically sets the
reading wavelength and factors.
Tests are continuously reviewed for simplified handling and to
make sure current legal requirements are followed. This can lead
to a change in the procedural instructions or an alteration to the
factors. If such an alteration is indicated on the test packaging,
update the instrument data according to the data supplied on the
process specification inside the test packaging.
For updating from the Internet, refer to Section 5.5.5.3, page 70.
1. Press Stored Programs in the Main Menu and select a

program.
2. Press Program Options and then Edit.
62
Standard programs
The Data list of the corresponding test will appear.

The process specification of the corresponding test contains
the new data (Wavelength, Factors, reading Ranges,
Conversion Factor for chemical Form etc.).
3. Highlight the corresponding line in the data overview and
press Edit.
4. Press OK and then Store after editing the test.
5.4.10.3 Program a new test
1. Press Stored Programs in the Main Menu.

2. Press Program Options and then New.
3. Use the alphanumeric keypad to enter the Program Number
indicated in the working procedure.
An overview of the data for the test to be programmed is
displayed.
the new data (Wavelength, Factors, reading Ranges,
Conversion Factor for chemical Form etc.).
4. Highlight the line containing the test specification that is to be
programmed, then press Edit and enter the data in the
appropriate program lines.
5. Press OK and then Store after editing the test.
5.4.11 Add stored programs to the favorite programs list

The "Favorites" menu simplifies test selection by creating a list of
the most frequently used tests from the Stored Programs
andUser Programs.
1. Press Stored Programs in the Main Menu. The "Stored

Programs" list will appear.
2. Highlight the selection by pressing it or Select by Number to
search for the program by number.
3. Press Add to Favorites and press OK to confirm.
The program can now be selected from "Favorite
Programs" menu in the Main Menu.
63
Standard programs
5.5 Barcode Programs

A special barcode reader in cell compartment (1) automatically
reads the barcode on the 13 mm cuvette/vial as the cuvette/vial
completes a single rotation. The instrument uses the barcode
identification to automatically set the correct wavelength for the
analysis and calculates the result immediately with the help of the
stored factors.
In addition, readings are recorded at 10 different positions during
a rotation. A special outlier-elimination program is run and then
the average of the measured values is calculated. Cell and round
cell faults and contaminations are detected and therefore a very
precise result is determined.
5.5.1 Complete a barcode test
1. Insert the light shield into cell compartment (2).

2. Prepare the barcode test according to the process
specification, and insert the cell in cell compartment (1).
• When a coded cell is placed in cell compartment (1)
(Figure 3, page 19), the corresponding reading program
is automatically activated in the Main Menu.
• Otherwise, press Barcode Programs in the Main Menu

and insert a zero cell (depending on process
specification) into cell compartment (1).
Note: For further information about the Help Guide ("Information" icon),
refer to Appendix A, page 141.
The reading is started automatically and the results are

displayed.
Note: To define a sample dilution, press the Dilution key in the toolbar.
To evaluate other cell tests and other parameters, insert the

prepared cell into the cell compartment and read off the result.
Note: The control bar displayed on the right of the screen shows the
relationship of the reading result to the measuring range. The black bar
shows the measured result independently of any dilution factor that was
entered.
64
Standard programs
5.5.2 Select the chemical evaluation form

The chemical form of the test result of a number of parameters
can be selected individually.
1. In the result display, press the unit (e. g. mg/L) or the
chemical representation of the evaluation form (e. g.
PO43––P).
A list of available evaluation forms appears.
2. Select the required form from the list. Press OK to confirm.
Another way of changing the standard setting is:

A list of available evaluation forms appear.
2. Select the required chemical form and press OK to confirm.
Note: The selected chemical form is displayed, but does not
become the default. To change the default, refer to Section 5.5.2.1.
5.5.2.1 Change of the default setting of the chemical form

1. Insert the zero cell or sample cell (corresponding to the
process specification) into the cell compartment.
3. A list of available evaluation forms appear. Select the new
default setting.
4. Press Save as Default.
The current result and all further readings will be displayed in the
new chemical form.
5.5.3 Basic test-specific and sample-specific data settings

Press Options to change test or sample specific settings.
65
Standard programs
Table 5 Barcode program options

Options Description

Store: On stores all measurement data automatically
Store Off/On
Store: Off does not store any measurement data.
Abs % Trans To switch to % transmittance or absorbance readings
Send Data icon /

To send data to a printer, computer or USB memory tick (USB A) or network.
Send Data
timed (e. g. reaction times, wait times, etc., can be exactly specified). When the specified time
Timer icon
has elapsed, an acoustic signal is emitted. The use of the timer has no influence on the reading
program.
A corrective dilution factor can be entered in order to take account of certain properties.
The number entered at the dilution factor prompt will be multiplied by the result to compensate
for the adjustment. For example , if the sample has been diluted by a factor of 2, enter 2. The
Dilution Factor Off/On
default setting of the dilution factor is turned off.
Note: When a dilution is in effect, the dilution icon will appear on the display.
Note: If undiluted samples are used, set the dilution factor off.
Chemical Form Some of the stored tests/methods allow to select the chemical form and the measuring range.
The function "Reagent Blank" is available for several barcode tests. This enables the reagent
blank value to be added to or subtracted from, the subsequent readings. The reagent blank
Reagent Blank value shifts the calibration curve along the y-axis, without changing the shape or gradient of the
curve. The effect corresponds to a y-axis intercept of the calibration straight line. This is clarified
by the following formula: concentration =[conc. factor) * Abs] - (reagent blank)
Edit To modify an existing program
Lot information All relevant manufacturing data is stored here.
Multiple determination This option allows the multiple determination of a sample.
Recall measurement
Recall saved measurement data, wave-length scans or time courses, see Section 5.3, page 43.
data
Instrument Setup mode For basic instrument data, refer to Section 5.2, page 27.
66
Standard programs
5.5.4 Sample blank
Turbidity and colour in the sample matrix can falsify the results of
a photometric analysis. The interference factors come from the
sample or are created by reactions with the reagents.
The influence of turbidity and/or colour can be eliminated or
reduced by taking a sample blank reading.
After the sample reading has been carried out, a special cell
(LCW919) with the sample blank is inserted in the cell
compartment. Measurement is automatic. The sample reading is
then corrected by adding or subtracting the blank value. The
result is shown in the display with the Blank Correction icon.
Some barcode tests do not require a sample blank value to be
determined, as turbidity and colour are dealt with during the test
procedure.
Note: For further information about the Help Guide ("Information" icon),
refer to Appendix A, page 141.
5.5.5 Update/edit barcode tests
5.5.5.1 Manual update of a barcode test

Note: Only in very few cases does the revision of a test require all test
specifications to be updated.
The various data types that determine a barcode test are

described in Table 6.
Table 6 Program update options

Program point Description
Name Name of the analysis parameter
Version An abbreviation assigned by the user or the version number, etc. is entered here
Exact definition of the test: the number of wavelengths at which readings are carried out, the
Measurement process number of absorbance readings needed, the keys to be used, any waiting periods between
readings, etc.
Formula Definition of the evaluation formulas with which the test result is calculated
The number of variables shown in the display depends on the definition of the measurement
Variables process and the formulas. Input of the numerical values of the wavelengths, factors, constants,
etc.
This function can be used to enter abbreviations and defined times for up to four timers.
Highlight the corresponding line and press Edit.
Timer 1, Timer 2,
The timers are activated or deactivated with the control boxes on the left of the display. In the
Timer 3, Timer 4
next column, a selection can be made from a list of names that describe the corresponding work
step. In the third column, the times for each active timer are entered.
67
Standard programs
Enter the Name

Check the process specification in advance to determine whether
this point needs to be changed.
1. Highlight the line containing Name and press Edit. Use the
alphanumeric keypad to enter the name indicated in the
process specification.
Enter the Version

1. Highlight the line containing Version and press Edit. Use the
alphanumeric keypad to enter the name indicated in the
process specification.
Enter the measurement process

For detailed information on entering the measurement process,
refer to Section 6.1.2, page 82.
1. Highlight the Measurement process line in the data
overview and press Edit.
2. Press Edit, select the sequence to be editted and press
Delete.
3. Press New and use the alphanumeric keypad to enter the
process indicated in the process specification.
Enter the Formula, Concentration Units, Designation,

Measuring Ranges
For detailed information on entering the Formula, refer to
this information needs to be changed.
1. Highlight the Formula line in the data overview and press
Edit.
2. Select the formula; press Edit, and enter the data (refer to
process specification) (for C1=, C2=, units, name,
measurement range limits, etc.). Press OK to confirm the
entry.
68
Standard programs
Enter the Variables (Factors, Wavelength and Coversion

Factors)
For detailed information on entering the Variables, refer to
1. Highlight the Variables line in the data overview and press
Edit.
2. Select the variable; press Edit, and enter the data (refer to
process specification) using the alphanumeric keypad (for
F1, F2, 11, U1 etc.). Confirm each entry with OK.
Abbreviation of Variables:
F1: Factor 1
F2: Factor 2
1: Wavelength 1
U1: Conversion factor 1 for the first chemical form
U2: Conversion factor 2 for additional chemical forms, etc.
.
Timer 1, Timer 2, Timer 3 and Timer 4:

The timers are activated or deactivated with the control boxes on
the left of the display. In the next column, a selection can be
made from a list of names that describe the corresponding work
step. In the third column, the times for each active timer are
entered.
3. When finished programming, press Store and then Cancel

to return to the "User Programs" menu.
4. Press Start to begin the test with the new data.
69
Standard programs
5.5.5.2 Manual check/revision of test data

If an incorrect entry was made during the data update and is not
recognized via the barcode of the cuvette/sample cell (e. g. an
incorrect measuring range), another option is available for
checking and if necessary revising, the test data.
1. Insert the light shield in cell compartment (2).

The instrument needs to be at the "Main Menu". Alternatively,
select 
Barcode Programs in the Main Menu.
process specification) into cell compartment (1).
3. Press Options>More>Edit.
A data overview is displayed, with the specifications of the
test that is to be revised.
4. Compare the displayed data with the data in the process
specification and edit the displayed data in accordance with
the process specification.
5.5.5.3 Update via the Internet
Update via USB memory stick

To obtain the software for the update from the Internet at
www.hach.com:
1. Go to http://www.hach.com.
2. Select download resources>software downloads.
3. Enter DR 3900 in "Search for documents".
4. Locate the appropriate download file.
5. Open the ZIP file and save the files to the USB memory stick
or PC. The files have to be unzipped prior to saving on USB
or PC.
6. Press Instrument Update in the "System Check" menu.
7. Connect the USB memory stick to the USB port (type A) on
the instrument, refer to Section 3.5, page 18.
8. Press OK. The link is established automatically and the
software is updated.
9. Press OK to return to the "System Check" menu.
Update via the web function

1. Connect the instrument to the network via an Ethernet cable.
2. Activate the Internet connection by selecting
Instrument Setup>PC & Printer (Section 5.2.7.4, page 39).
70
Standard programs
3. Press Web Function on the toolbar.

4. Press Instrument Update.
The update will start automatically.
Note: When the instrument software has been updated, a prompt to
restart the instrument is displayed.
5.5.5.4 Program a new test

1. The instrument needs to be at the "Main Menu" or press
Barcode Programs in the Main Menu.
The message "Program not available" is shown, followed by

a data overview with the specifications of the test that is to be
revised.
the new data (Wavelength, Factors, Reading Ranges,
Conversion Factor for chemical form etc.).
3. Highlight the corresponding line including the specifications
of the test that is to be programmed and press Edit.
4. To finish programming, press Store and then Cancel to
return to the Main Menu.
5. Press Start to begin the test with the new data.
71
Standard programs
72
Section 6 Expanded programs
6.1 User programs

User programs provide the opportunity to complete "made to
measure" analysis.
The "User Programs" database is empty when the instrument
leaves the factory and is used to accommodate programs created
by users for specific needs. User programs with 13 mm round
cells can only be performed with the empty cells LCW906. Here
are a few examples of entries:
• Programming of user-created procedures. 
The analysis procedure must be developed first, before it can
be programmed. The user must define or determine the
program sequences, absorbance formulas, reading
wavelengths, factors, measuring range limits, etc.
• Modified tests
• Assignment of user programs to the "Favorites" menu for
frequently used tests.
• Creation of a specific selection of methods and tests.
1. Select User Programs in the "Main Menu"
2. Select Program Options.
The Program Options menu contains several input and editing
options (Table 7):
Table 7 Program options for user programs

Options Description
Select New to program a new user program.
New Note: The first time Program Options is selected, only the New option is available. The other
options remain inactive (grey) until the first program has been created.
Add to favorites Select Add to favorites to add an existing user program to the Favorites list.
Edit Select Edit to modify an existing program

Select Delete to remove a program from the list of user programs. The program will be
Delete
simultaneously deleted from the Favorites list.
73
Expanded programs
6.1.1 Program a user method

All input steps and their significance and options are explained in
the following sections.
1. Select New in the "Program Options" menu.
Program Number:
Specific test number, with which the program can subsequently
be called up from the selection list in the User Programs menu
or the Favorites menu.
2. Use the alphanumeric keypad to enter a program number
between 9000 and 9099. The lowest available number
appears automatically.
3. Press OK.
Note: If a program number is already allocated to another user
program, a query appears asking whether the present program is to
be replaced. If OK is pressed, the existing program is overwritten.
Program name:
4. Use the alphanumeric keypad to enter a program name. The
name can be a maximum of 28 characters long.
5. Press Back to go back to the previous program point or
press Next to continue with the input of the program data.
Program type:
6. Select the required program type (Table 8) and press Next.
7. If the options Single Wavelength (Section 6.1.1.1, page 75)
or Multi-Wavelength (Section 6.1.1.2, page 76) are
selected, define the programming parameters:
• Unit
• Wavelength
• Calculation formula
• Wavelength 1x
• Concentration factor Kx
• Concentration solution
• Chemical form
• Calibration equation
For more information on free programming parameters refer to
74
Expanded programs
Table 8 Program descriptions

Program type Description
Single Wavelength Readings at a defined wavelength

In the Multi Wavelength mode, absorbance values can be measured at up to four wavelengths
Multi Wavelength
and the results can be mathematically processed to obtain sums, differences and relationships.
A high degree of flexibility results in individual options to create a user program. The free
Free programming
programming is an expanded form of test or method programming.
6.1.1.1 Single wavelength settings

The following parameters can be defined when selecting the
single wavelength mode:
Units:
1. Select the required unit from the list.
2. Press Continue.
Note: Units of measure not included in this list can be added in the edit
program under Program Options, Edit. Select Units, Edit and then
New.
Wavelength (single wavelength program type):

1. Enter measured wavelength.
The entered wavelength must be in the range from
320–1100 nm.
2. Press Continue.
Concentration resolution (number of decimal places)

1. Select the required number of decimal places to be
displayed after the decimal point from the displayed list.
2. Press Continue.
75
Expanded programs
Chemical form:
1. Enter the chemical formula used in the display to represent
the analysis parameter.
2. Press Continue.
6.1.1.2 Multi wavelength settings

When selecting the Multi-Wavelength mode, the following
parameters can be defined:
Units:
1. Select the required unit from the list.
2. Press Continue.
Note: User-specific unit not included in this list can be added in the edit
program under Program Options, Edit. Select Units, Edit and then
New.
Absorbance formula (Multi Wavelength program type):

Define the wavelengths here and the coefficients used in the
formula. The absorbance formula defines the calculation for the
multi wavelength reading.
1. Press Formula.
2. Select the formula relevant to the programming from the
displayed list.
List of available absorbance formulas

A1 is the absorbance at wavelength 1,
A2 is the absorbance at wavelength 2 and so on
K1 is the factor at wavelength 1,
K2 is the factor at wavelength 2 and so on
If a subtraction has to be performed, the factors can be entered
with a minus sign.
76
Expanded programs
Wavelength x:
1. Press the x buttons.
The number of wavelengths results from the calculation
formula.
2. Enter the required wavelengths.
3. If necessary, repeat until all values for the formula have been
entered.
The wavelengths must be in the range from 390–1100 nm.
Concentration factor Kx
The concentration factor Kx is a multiplication factor to convert
absorbance values into concentration values.
1. Press the Kx buttons.
The number of the concentration factors results from the
calculation formula.
2. Enter the required factor.
3. If necessary, repeat until all the values for the formula have
been entered.
5. Press Continue.
Note: Up to 5 digit place values can be entered. A maximum of 4
decimal places are possible.
Concentration resolution (number of decimal places)

1. Select the required number of decimal places to be
displayed after the decimal point from the list.
2. Press Continue.
Chemical form:
1. Enter the chemical formula used in the display to represent
the analysis parameter.
2. Press Continue.
77
Expanded programs
6.1.1.3 Calibration settings for single and Multi Wavelength mode

A method is calibrated by determining the absorbance values of
several standard solutions of known concentration.
Create and save a calibration curve by entering values or
measuring standards or entering formulas (Table 9).
Table 9 Calibration settings

Option Description
Create a calibration table by entering the concentration values and the corresponding
absorbance values of the standard solutions be measured. The absorbance values are applied
Enter values
in a diagram against the concentrations of the standard solutions. The calibration curve is
graphically presented (page 78).
Create a calibration table by entering the concentration values of the standard solutions to be
measured and then determining the corresponding absorbance values by measuring the
Measure standards
standard solutions. The absorbance values are applied in a diagram against the concentrations
of the standard solutions. The calibration curve is graphically presented (page 79).
If it is possible to determine the calibration curve from the mathematical relationship between
concentration and absorbance through linear regression or another curve adaptation, the
Enter formula
respective formula (linear, polynomial 2nd or 3rd order can be selected from a list and the
corresponding factors can be entered (page 80).
Calibration by "Enter values"
1. Select Enter values.

2. Press Continue.
3. Press +.
4. Enter the standard concentration.
6. Enter the corresponding absorbance value.
8. If necessary, repeat until all values have been entered.
Note: To change a value in the table, mark the corresponding row. Press
the key of the unit (e. g. mg/L) or Ext. Enter the changed value.
78
Expanded programs
9. Press Graph.
The entered data is shown as a calibration curve.
Note: The correlation coefficient (r2) is shown on the left below the
axes.
The linear equation corresponds to the standard setting.

10. Press Next graph.
The presentation changes to the polynomial curve of the
second order.
The presentation changes to the polynomial curve of the third
order.
12. Press Force 0 to change the setting from Off to On. The
curve then passes through the origin of the coordinate
system.
Note: This may have an adverse effect on the correlation coefficient
(r2).
13. Press on table to return to the values table.

14. When the table has been completed and the curve type has
been chosen, press Done when the graph is displayed or
Exit when the table is displayed. Go to Section 6.1.1.4,
page 81.
Calibration using "Measure standards"
1. Press Measure standards.

2. Press Continue.
3. Press +.
4. Enter the standard concentration.
6. If necessary, repeat until all values have been entered. A
maximum of 24 values can be entered.
Note: To change a value in the table, mark the corresponding row.
Press the key of the unit (e. g. mg/L). Enter the changed value.
7. Insert the zero solution into the cell compartment.

8. Press Zero.
9. Mark the row with the first standard concentration and insert
the respective cell.
10. Press Read.
11. If necessary, repeat until all standard solutions are
measured.
79
Expanded programs
The entered and measured data is displayed in the table.

Note: To delete a standard concentration, highlight the appropriate line
and press the Delete icon.
The timer icon shown in the display helps to make sure, when
necessary, that the steps of an analysis are correctly timed (e. g.
reaction times, wait time, etc., can be exactly specified). When
the specified time has elapsed, an acoustic signal is emitted. The
use of the timer has no influence on the reading program.
12. Press Graph.

The entered and measured data is shown as a calibration
curve.
Note: The correlation coefficient (r2) is shown on the left below the
axes.
The linear equation corresponds to the standard setting.

The presentation changes to polynomial curve of the second
order.
The presentation changes to polynomial curve of the third
order.
15. Press Force 0 to change the setting from Off to On. The
curve then passes through the origin of the coordinate
system.
Note: This may have an adverse effect on the correlation coefficient
(r2).
16. Press Table to return to the values table.

17. When the table has been completed and the curve type has
been chosen, press Done when the graph is displayed or
Exit when the table is displayed. Go to Section 6.1.1.4,
page 81.
Calibration using "Enter formula"
1. Press Enter formula.

2. Press Continue.
80
Expanded programs
3. Press the Formula key.

A list of available formulas (linear and 2nd and 3rd order
polynomial order) is displayed. Up to 4 coefficients can be
entered, depending on the selected formula.
4. Press the required formula.
According to the selected formula, the required factors (a, b,
c...) are shown.
5. Press the factors keys and enter the corresponding values.
6. Press OK to confirm each factor entry.
Note: The coefficients can have 5 digits and can have a positive or a
negative sign.
6.1.1.4 Store a user program
The input of the basic data is complete. An overview of the

variable program data is displayed.
1. To enter more specifications or change existing ones,
highlight the appropriate line and press Edit.
2. Select Store to save the user program.
6.1.1.5 Additional user-defined parameters and functions

In addition to the previously defined basic data, additional
parameters and functions can be defined for user programs:
• measuring range upper and lower limits
• timer functions
• chemical forms
Upper and lower limits of the measuring range

It is possible to enter a maximum (upper) and minimum (lower)
reading value. An error message is displayed if a reading is
above the upper limit or below the lower limit.
1. Mark the Upper limit or Lower limit row.
2. Press Edit.
3. Select On.
4. Press 0.000 and enter the measuring range limit.
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Timer 1 / Timer 2 / Timer 3 / Timer 4:

Up to four time intervals can be defined as timers with this
function. Descriptions of the individual work steps, such as
shaking, waiting, and stirring can be allocated to the timers.
1. Mark the Timer x row.
2. Press Edit.
Activate or deactivate the timers using the check box in the
lower area of the display.
3. Activate a timer.
4. Press Timer.
5. Select the designation from the displayed list that describes
the respective process and confirm with OK.
6. Press 00:00.
7. Enter the required time interval (in mm:ss) and confirm with
OK.
Chemical form 2 / chemical form 3 / chemical form 4:

If a chemical form 1 was defined, up to three additional
alternative forms can be entered.
1. Mark the Chemical form x row.
2. Press Edit.
Activate or deactivate the chemical form using the check box
in the left area of the display.
3. Press the left key to enter an additional chemical form.
5. Press the right key to enter the conversion factor for this
additional chemical form.
7. Press Store to save the program data. Press Cancel to
return to the Main Menu.
6.1.2 "Free programming" program type

Free programming is an advanced option for entering original
user-developed methods. The data overview contains the
specifications of the programmed tests. Each entry option can be
activated by pressing the respective row and can be changed
using Edit (refer to Table 10).
1. Select New in the "Program Options" menu.
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Program Number:
Specific test number, with which the program can subsequently
be called up from the selection list in the User Programs menu
or the Favorites menu.
2. Enter a program number between 9000 and 9099. The
lowest available number appears automatically.
3. Press OK.
Note: If a program number is already allocated to another user
program, a query appears asking whether the present program is to
be replaced. If OK is pressed, the existing program is overwritten.
Program name:
4. Use the alphanumeric keypad to enter a program name. The
name can be a maximum of 28 characters long.
5. Press Back to go back to the previous program point or
press Next to continue with the input of the program data.
Program type:
6. Select Free programming and then press Continue.
7. Highlight the line containing the program point that is to be

edited or defined and press Edit.
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Table 10 Definitions of the program points

Program point Description
Name Name of the analysis parameter
Version An abbreviation or version number assigned by the user is entered here.

Exact definition of the test: the number of wavelengths at which readings are carried out, the
Measurement process number of absorbance readings needed, the keys to be used, any waiting periods between
readings, etc.
Formula Definition of the formulas with which the test result is calculated.
The number of variables shown in the display depends on the definition of the measurement
Variables process and the formulas. Input of the numerical values of the wavelengths, factors, constants,
etc.
This function can be used to enter abbreviations and defined times for up to four timers.
Highlight the corresponding line and press Edit. The timers are activated or deactivated with the
Timer 1, Timer 2,
control boxes on the left of the display. In the next column, a selection can be made from a list of
Timer 3, Timer 4
names that describe the corresponding work step. In the third column, the times for each active
timer are entered.
6.1.2.1 Measurement process

The measurement process defines the handling and the readings
of the test:
• At which and how many wavelengths should readings be
completed
• How many absorbance readings must be completed?
• When should the zero reading and the sample reading be
completed?
• Are waiting times necessary between readings?
• Should individual program sequences be repeated?
The elements of a measuring sequence, such as zero and
sample readings and the timer(s) (reaction times, waiting times,
etc.) are individually defined.
6.1.2.2 Enter a new element of a measuring sequence

Note: Each component of the measurement process must be entered in
the order in which it will be completed.
1. Mark the Measurement process row in the data overview.

2. Press Edit.
3. Press Edit.
4. Press New.
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Content and definition of the keys
[Z] key / Zeroing

1. Press [Z] to program a zero reading.
3. Press New.
4. Press Zeroing and enter the wavelength for which the zero
reading is to be performed.
5. Press OK and confirm the entry.
6. Press OK.
7. If zero readings are to be carried out at a number of
wavelengths, repeat the steps starting with 1 for each
wavelength.
Note: The entered reading sequence is displayed.
Process Timer key

1. Press New.
2. Press Process timer to enter wait, reaction or handling
times.
4. Press OK.
Note: This time is integrated into the measurement process.
[R] key / Reading...

1. Press New.
2. Press [R] to program a reading of the substance that is to
be analyzed.
4. Press New.
5. Press Read...and enter the wavelength for which the
measurement is to take place.
7. Press OK.
8. If readings are to be completed at a number of wavelengths,
repeat the steps starting with 1 for each wavelength.
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Key { }
Elements of the reading sequence that are to be repeated are
placed in brackets.
The left bracket { marks the start of the sequence to be repeated
and the right bracket } marks the end.
Note: The showing the right bracket remains inactive until a left bracket
is entered.
1. To start the repetition sequence, press New.

2. Press {.
3. Press the key that defines the sequence that is to be
repeated: [Z] or [R].
4. Press OK.
5. Press New.
6. Press Zeroing or Read..., and enter the wavelength for
which the measurement is to occur.
8. Press OK.
Define additional steps that are to be included in the
repetition sequence.
9. At the end of the repetition sequence, press New.
Define the last step that is to be included in the repetition
sequence. Do not press OK.
10. Press }.
11. Press OK.
Note: If an action such as a zero reading recurs at different stages
of a reading sequence, the series of actions is numbered
sequentially (e. g. Z1, Z2, etc.).
Delete an element of a measuring sequence

1. Mark the respective row.
2. Press Delete.
The element is deleted.
Insertion of an element of a measuring sequence

1. Mark the row below the reading sequence to be inserted.
2. Press New.
A new element can be entered at the selected position.
3. When the input is complete, press OK in the "Measurement
Process" display.
The measurement process is displayed in the overview.
4. Press OK.
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6.1.2.3 Enter the calibration formula (evaluation formula)

The calibration formula (evaluation formula) defines the
calculation and display of intermediate and final results. The
previously defined elements of the measuring sequence are the
basis for calculating the concentrations.
Enter calibration formula C1

1. Mark the Formula row in the data overview.
2. Press Edit.
3. Mark the C1 row: Off.

4. Press Edit.
5. Mark C1: Out.

6. Press Edit.
The display changes to C1: On.
7. Mark the next row C1=, to define the formula.
8. Press Edit.
Refer to Table 11 for detailed information on the Edit formula

keys.
Note: The evaluation formula is built up successively in the display in
accordance with the input.
Note: The arrow key deletes the most recently entered element of the
formula.
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Table 11 Edit formula key descriptions

Screen Key Description
Press the Abs/Variables key to select, from the

displayed list, the required element of the defined
Abs/Variables measuring sequence and therefore the corresponding
reading wavelength, so that this can be taken into
account in the formula.
New Number Press New Number to enter a new factor or constant.
Press +–x to enter a mathematical operation.

Select the operation and press OK to confirm.
The available choice of mathematical operations
depends on the defined formula.
This means that functions such as "( )" or "ln"/"log" etc.
are only active if a term in parentheses or the calculation
of a logarithm is mathematically permissible in the
defined formula (this also applies to the basic
mathematical operations).
The following mathematical operations are available:
+–×
• + (Addition)
• - (Subtraction)
•  (Division)
• x (Multiplication)
• ^ (Exponent)
• Ln (Natural logarithm)
• Log (Common logarithm)
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Table 11 Edit formula key descriptions(Continued)

Screen Key Description
Press >=< to include logic statements/links/conditions in
the formula. The following functions are available:
= (Equal to), < (Less than), > (Greater than), <= (Less
than or equal to), >= (Greater than or equal to), IF,
THEN, ELSE
>=< When the evaluation formula C1 has been entered
completely, press OK to confirm. Press OK again to
return to the Formula display.
When the formula C1 has been entered and confirmed,
the parameter name, upper and lower limits of the
measuring range and display result (yes, no) can be
entered.
Eentering the next calibration formula (C2 or Cn)

1. Mark C2: Out.
2. Press Edit.
3. Mark C2: Out.
4. Press Edit.
The display switches to C2: On.
5. Mark the next row C2= to define the formula.
6. Press Edit.
In addition to the keys described in Table 11, only one other

function is available:
Select Conc. key

1. If an already defined formula, in this case C1, is to be taken
into account in the formula for C2, press Select conc.
2. Enter the numbering of the equation (e. g. 1 for C1).
Cn can now be linked with a mathematical operation.
Note: The Cn concentrations that are to be calculated are numbered in
sequence: C1, C2, C3, etc.
Note: When the first Cn formula has been defined, the Formula list is
automatically extended by Cn+1.
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6.1.2.4 Enter variables
1. Mark the Variables row in the data overview.

2. Press Edit.
3. Select the variable to be edited.
4. Press Edit. and enter the data (refer to process
specification) (for F1, F2, 1, U1 etc.)
5. Press OK to confirm each entry.
Abbreviation of Variables:
F1: Factor 1
F2: Factor 2
 1: Wavelength 1
U1: Conversion Factor 1 for the first chemical form
U2: Conversion Factor 2 for an additional chemical form etc.
6.1.2.5 Save a free programming user program

1. Press Store to save the entered data.
The data can be stored under any data point (measurement
sequence, formula, timer, etc.).
6.1.3 Select a user program
1. Press User Programs in the Main Menu.

An alphabetical list of user programs with program numbers
is displayed.
Note: Use the scroll bar to scroll through the list quickly.
Note: Highlight the selection by pressing it or Select by Number to

2. Press Start to run the program.
6.1.4 Add, edit and delete user programs from the favorites list
The most frequently used tests/methods in the "User Programs"
menu can also be added to the list of favorites to simplify their
selection.
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1. Press User Programs in the Main Menu.

The User Programs list will appear.
2. Highlight the selection by pressing it or Select by Number to
3. Press Program Options.

Using Add to favorites, add the selected program to the
favorite list.
Use Edit to edit the selected program.
Use Delete to delete the selected program.
Note: If the stored program is deleted in User Programs, it will also
be deleted in Favorites Programs.
6.1.4.1 Add to favourites
1. Press Add to favourites.

The program is added to the Favorites.
6.1.4.2 Edit
1. Press Edit.
An overview of the specifications of the programmed test is
displayed. More information about the input options is
provided in the Section 6.1.2, page 82.
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6.1.4.3 Clear Entry
1. Press Delete.
The program will be deleted from the list of user programs.
6.2 Favorites
The most frequently used tests/methods in the Stored Programs
menu and the User Programs menu can also be added to the list
of favorites to simplify their selection.
To add Stored Programs and/or User Programs to the favorites
list or the favorite programs, refer to Section 6.1.4, page 90.
6.2.1 Recall a favorite program
1. Press Favorite Programs in the Main Menu.

The "Favorite Programs" list will appear.
2. Highlight the selection by pressing it.
Note: Press Select by Number to search for the program by

number. Use the alphanumeric keypad to enter the test number
(program number) and confirm the input by pressing OK.
3. Press Start.
6.2.2 Delete a favorite program
1. Press Favorite Programs in the Main Menu.

The "Favorite Programs" list will appear.
2. Highlight the selection by pressing it.
Note: Press Select by Number to search for the program by

number. Use the alphanumeric keypad to enter the test number
(program number) and confirm the input by pressing OK.
3. Press Remove program.
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Note: If a Favorite Programs is deleted, it will stay in the User
Programs or Stored Programs.

6.3 Standard Addition - monitoring/checking results

The accuracy of readings (their correspondence with the actual
concentration of the analyte in the sample) and their precision
(correspondence of the reading results obtained from several
samples containing the same concentration of the test analyte)
can be determined or improved using the standard addition
method
This method (also known as standard-addition or spiking) serves
to detect sample-specific faults:
• The analysis was inaccurate by substances in the sample
(sample matrix effect),
• defective measuring device,
• impure reagents.
Method:
Add a defined quantity (concentration) of the substance to be
tested to the sample as a standard solution. The detection rate
(concentration of the added standard solution) should be close to
100%.
Reading of successful standard addition

Detection rate = Expanded value after successful standard
addition
Detection rate Conclusion

Probability that the reading results are correct
100%
is high.
Assumption: The analysis was inaccurate by
substances in the sample (sample matrix
effect)
Test to determine whether a sample matrix
 100%
effect is present:
Use distilled water instead of the sample. Add
standard solution as described in the
procedure.
Ions in the sample are
interfering with the
100%
analysis, causing false
results to be obtained.
 100% No interference ions.
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Measures to identify other interference factors:
Checklist:
1. Check if the procedure is completed correctly:
a. Are the reagents added in the correct order?
b. Is enough time allowed for colour development?
c. Is the correct glassware in use?
d. Is the glassware clean?
e. Does the test require the sample to be at a certain
temperature?
f. Was the pH of the sample in the correct range?
g. Is the pipette volume correct?
2. Check the reagents. Repeat the standard additions using
fresh reagents.

The originally used reagents were defective.
Check the standard solution:
100% Repeat the standard addition procedure with
a freshly prepared standard solution and
distilled water.
The originally used
100 % standard solution
was deficient.
If none of these measures resolves the problem, please contact

the manufacturer.
6.3.1 Complete a standard addition

Carry out the standard addition in accordance with the
corresponding procedure.
There are two different methods:
Maximum Volume (standard addition)

A defined volume of a standard solution of known concentration
is incrementally added to a previously tested sample and the
sample is newly measured after every standard addition.
Sample volume:
A defined volume of a standard solution of known concentration
is added to the volume of sample specified in the procedure and
the sample is measured after each addition. In most cases three
different standard solutions are prepared and the procedure is
repeated for each of them.
Note: The units and chemical forms used for the sample are used for the
standard solutions. Be careful to make sure that to use the correct units
for subsequent entries.
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Maximum Method Volume/Sample volume

1. Press Stored Programs in the Main Menu.
2. Select the required program.
3. Press Start.
4. Analyze a sample without added standard solution in
accordance with the instructions in the Procedures Manual.
When the reading is complete, leave the sample cuvette/cell
in the cuvette/sample cell compartment.
5. Press Options>More>Standard Addition.
An overview of the data of the standard addition procedure is

displayed.
6. Press OK to accept the standard values for standard
concentration, sample volume (total) and standard addition
volume.
7. Press Edit, to change these values.
8. Press the key of the value to change it. Use the

alphanumeric keypad to change the value.
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10. Press the keys to change the standard addition volumes.

Use the alphanumeric keypad to enter the new data.
Description of the table of readings

• The first column shows the standard addition volume. 0 mL
stands for a sample containing no added standard.
• The second column shows the reading of the samples with
and without added standard.
• The third column shows the detection rate of the added
standard.
Note: The black highlighted line is active.
The reading of the sample in the instrument, without added

standard, is automatically shown under 0 ml.
12. Prepare the standard addition solution as described in the
procedure.
13. Press the standard addition volume in the table.
14. Insert the cell with the respective addition volume into the
cell compartment.
15. Press Read.
Repeat the procedure with all the other standard addition
solutions.
16. After all the standard addition solutions have been

measured, press Graph.
The regression line through the standard addition data points
is displayed.
The correlation coefficient r2 indicates how close the data
points are to the line.
If the correlation coefficient = 1, the curve is linear.
The concentration shown above the curve is the estimated
concentration of the sample without the added standard.
Note: In the Graph menu, the name on the Graph key switches to
Table. Press Table to display all the data in the table again.
17. Press Ideal line to display the relationship between the

added standard solutions and the ideal line (detection rate
100%).
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6.4 Single Wavelength (absorbance, concentration and transmittance

readings)
The Single Wavelength mode can be used in three ways. For
sample readings at a single wavelength, the instrument can be
programmed to measure the absorbance, % transmittance or
concentration of the analyte.
• Absorbance readings: The light absorbed by the sample is
measured in absorbance units.
• % Transmittance measures the percent of the original light
that passes through the sample and reaches the detector.
• Turning on the concentration factor allows selection of a
specific multiplier for converting absorbance readings to
concentration. In a graph of concentration versus the
absorbance, the concentration factor is the slope of the line.
6.4.1 Set up Single Wavelength mode

1. Press Single Wavelength in the Main Menu.
2. Press Options for Parameter Setup.
Table 12 Options for Single Wavelength

Options Description
Recall measurement Recall saved measurement data, wavelength scans or time courses, refer to Section 5.3,
data symbol page 43.
% Trans/Abs To switch to % transmittance, concentration or absorbance readings

To input the wavelength setting. Use the alphanumeric keypad to enter the reading wavelengths.
Wavelength
The entered wavelength must be in the range from 320-110 nm.
Timer icon
program.
Concentration Factor Multiplication factor for converting absorbance values into concentration values.
Concentration
To select the number of decimal places.
Resolution
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Table 12 Options for Single Wavelength(Continued)

Options Description
data page 43.
Instrument Setup mode Basic data of the instrument, refer to Section 5.2, page 27.
Concentration Factor:
1. Press Concentration Factor: Off in the "Options" menu.
2. Press On to highlight this feature.
3. Press Factor and enter the factor.
4. Press Unit and select the corresponding units for
concentration from the displayed list.
Concentration Resolution:
1. Press Concentration Resolution in the "Options" menu.
2. Select the resolution.
4. Next press Return to return to the results screen.
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6.4.2 Take single wavelength readings (single reading)

2. Press Zero.
Note: The Read key is only active after the zero reading has been
completed.
3. Insert the analysis cell into the cell compartment.

4. Press Read.
6.5 Multi Wavelength mode – readings with more than one wavelength
In the Multi Wavelength mode, absorbance values can be
measured at up to four wavelengths and the results can be
mathematically processed to obtain sums, differences and
relationships.
• Absorbance readings: The light absorbed by the sample is
measured in absorbance units.
• % Transmittance measures the percent of the original light
that passes through the sample and reaches the detector.
• Turning on the concentration factor allows selection of a
specific multiplier for converting absorbance readings to
concentration. In a graph of concentration versus the
absorbance, the concentration factor is the slope of the line.
Concentration is calculated using a single factor for each
wavelength, which is input by the user.
6.5.1 Set the Reading mode at different wavelengths

Press Multi Wavelength in the Main Menu. Press Options for
Parameter Setup.
Table 13 Multi wavelength setup options

Options Description
data symbol page 43.
% Trans/Abs To switch to % transmittance, concentration or absorbance readings
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Table 13 Multi wavelength setup options(Continued)

Options Description
To input the wavelength setting. Use the alphanumeric keypad to enter the reading wavelengths.
Wavelength
The entered wavelength must be in the range from 320–1100 nm.
Timer icon
program.
Concentration Factor Multiplication factor for converting absorbance values into concentration values.
Concentration
To select the number of decimal places.
Resolution
Absorbance Formula Calculation basis for evaluating samples
Recall measurement
data
Absorbance Formula:
1. Press Absorbance Formula.
2. The formula selected in the top key determines the number
of wavelength and coefficient keys that will appear below.
3. Press the key to change the calculation formula.
4. Select a formula from the displayed list.
5. Press OK.
When a new formula is selected, the number of variables
below changes to match.
The following formulas are available:

K 1 A 1 + K 2 A 2
K 1 A 1 + K 2 A 2 + K 3 A 3
K 1 A 1 + K 2 A 2 + K 3 A 3 + K 4 A 4
K 1 A 1 / K 2 A 2
(K 1 A 1 + K 2 A 2 ) / K 3 A 3
(K 1 A 1 + K 2 A 2 ) / (K 3 A 3 + K 4 A 4 )
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A 1 refers to the absorbance at wavelength 1

A 2 refers to the absorbance at wavelength 2, etc.
K 1 refers to the coefficient at wavelength 1
K 2 refers to the coefficient at wavelength 2, etc.
If a subtraction has to be completed, the factors can be entered
with a minus sign.
6. Press the "x" keys to change the wavelengths.

7. Enter the required wavelength factor using the alphanumeric
keypad.
9. To change a coefficient, press one of the "KX" keys.

10. Enter the required coefficient using the alphanumeric
keypad.
Note: Up to 5 digit number values can be entered! A maximum of
four decimals places are possible.
Concentration Factor:
1. Press Concentration Factor: Off in the "Options" menu.
2. Press On to highlight this feature.
3. Press Factor.
4. Enter the factor with which the absorbance measurement is
to be multiplied.
5. Press Unit.
6. Select the respective concentration unit from the displayed
list.
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Concentration Resolution:
1. Press Concentration Resolution in the "Options" menu.
2. Select the resolution.
4. Press Return to return to the results screen.
6.5.2 Complete a reading in the Multi Wavelength mode

2. Press Zero.
Note: The Read key does not become active until the zero reading
has been completed.
3. Insert the analysis cell into the cell compartment.

4. Press Read.
6.6 Wavelength Scan mode – recording of absorbance and transmission

spectrums
In the Wavelength Scan mode, the absorbance of the light in a
solution over a defined wavelength spectrum is measured. While
the scan is taking place, the cell compartment cover must be
closed. Therefore, a scan of the round cell compartment is not
possible.
The reading results can be displayed as a curve, as percentage
transmittance (%T) or as Absorbance (Abs). The collected data
can be printed as a table or a curve.
The data is available for formatting changes. These include
automatic scaling and zoom functions. Maximum and minimum
values are determined and shown as a table.
The cursor can be moved to any point on the curve for the
purpose of reading off the absorbance or transmittance value and
the wavelength. The data associated with each data point can
also be shown as a table.
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6.6.1 Set up the wavelength scan

Press Wavelength Scan in the Main Menu. Press Options for
Parameter Setup.
Table 14 Options during wavelength scan

Option Description
More Further options are displayed
Folder icon To store the scan data

From the displayed list of stored scans, a record is selected for use as a reference
scan/superimposed scan. This can be highlighted or shown in the background in comparison
Reference Off/On with the actual measured scan.
Note: This option is only available when there are stored scans with the same wavelength range
and step.
 To enter the wavelength spectrum and the scan interval
Enables the user to switch the display back and forth between the scan data tables
Select View (wavelength/absorbance) and the graph of the curve.
Note: "Select View" will be activated after the first reading.
To select Track or Peak/Valley. The selection for this menu item determines to which points on
Cursor Mode
the graph the cursor moves.
Send Data To send data to a printer, computer or USB memory stick (Type A)
Integral: On/Off The integral gives the area and the derivative of the integral gives the original function
Scale: In the automatic Scaling mode, the y-axis is automatically adjusted so that the total scan
is displayed. 
Scale & Units
The manual Scaling mode allows sections of the scan to be displayed. 
Units: Choice of absorbance or transmittance.
Recall measurement
data
Setting of the wavelength

1. Press the key in the "Options" menu to select the
wavelength range and the wavelength step.
2. Press the upper left key to select the lower wavelength.
3. Enter the lower wavelength.
5. Press the upper right key to select the upper wavelength.
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6. Enter the upper wavelength.

Note: Do not select the same wavelength for minimum and
maximum.
8. Highlight the required wavelength step.

Note: The selection of the interval influences the duration and
resolution of the Wavelength Scan. Scans with a high resolution
take longer than scans with a low resolution. The larger the
increments of the intervals selected, the faster the scan runs. In
contrast, the resolution of the determined data decreases. 
In summery maximally 780 measuring steps can be accomplished
during a Scan.
Note: The maximum wavelength adjust automatically if the

difference between the maximum and minimum wavelength is not a
multiple of the interval.
9. Press OK to return to the Scan mode.

Selected parameters are displayed along the graph‘s x-axis.
Select view (display table)

1. Perform a Wavelength Scan (Section 6.6.2, page 105)
2. Press Display table in the "Options" menu.
3. A table with the results is displayed.
4. To return to the graph press Options and then View Graph.
Cursor Mode
1. Press Cursor mode: tracking in the "Options" menu.
The selection for this menu item determines what data is
displayed in the table.
2. Highlight Track or Peak/Valley.
4. Press Return to return to the Scan mode.
Integral
The Integral applies to the whole wavelength range of the scan.
1. Press Integral: Off in the "Options" menu.
2. Highlight On to show the Integral.
3. To find the integral of other wavelength ranges, change the
wavelength range and scan again.
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Note: The Integral is shown instead of the date on the display.
Note: For the next scan reading the setting for the Integral will be On.
Scale & Units

1. Press Options>More..>Scale & Units.
2. Highlight the required units (Abs or %T).
3. Highlight Auto or Manual scaling on the graph's y-axis.
Note: When selecting the manual scaling, the limit values y min and y max
can be entered using the alphanumeric keypad. The graph is adjusted to
display only the values in the selected range. If automatic scaling is
selected, the instrument sets the limits automatically so that the total
range can be displayed.
4. Press OK.
6.6.2 Wavelength scan reading

After selecting all scan parameters, record a base line (initial zero
reading). If a scan parameter is changed, a new base line must
be recorded. When the baseline has been scanned, the
instrument is ready to scan one or more samples.
1. Press Wavelength Scan in the Main Menu.

2. Insert the zero solution cell into the cell compartment and
close the cell compartment cover.
Note: The cell compartment must be closed via the cell
compartment cover during a scan. Therefore, no scans can be
performed with 13-mm round cells.
3. Press Zero.
"Zeroing" is shown below the graph as the baseline scan
begins.
4. Insert the prepared analysis cell into the cell compartment
and close the cell compartment cover.
5. Press Read.
Under the graph, the display "Reading..." appears, and a
graph of the absorbance or transmission values for the
scanned wavelengths is continuously displayed.
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The Wavelength Scan is complete, if

• the graph is shown full-size,
• the scaling of the x-axis occurs automatically,
• the Cursor functions in the vertical navigation bar are
highlighted.
• a signal sounds
6.6.2.1 Navigation of the wavelength scan graph or a wavelength scan analysis
Table 15 Navigating the wavelength scan

Cursor function/
Description
Zoom function
Curve Icon Choice of Cursor mode Peak/Valley (cursor moves between minimum/maximum absorbance
(Choice of Cursor mode) values) or Cursor mode Tracking (cursor moves over each data point of the scan).
The arrow keys (right/left) are used to move the cursor (depending on the selected mode) to the
next data point. The data of the data point (wavelength/absorbance or transmittance value) are
Arrow keys highlighted on the x and y axes.
Note: Press any point on the curve to display the associated data.
This function is used to magnify the section of the curve in the vicinity of the cursor. The original
Zoom Icon
curve size can be restored by pressing the zoom icon again.
6.6.3 Work with reference scans

There are two options to work with Reference Scan:
First option:
1. Press Reference: Off in the "Options" menu to select
another scan to display on the same screen with the current
scan. Highlight the required scan number and press
Highlight reference.
Note: After selecting a reference scan the Reference: Off key in the
"Options" menu turns into Reference: On.
Note: Only scans that have the same wavelength range and step
can be displayed using the overlay option. This process can be
repeated until all matching scans are displayed.
2. The reference curve is shown in orange. The absorbance or

transmittance value and the associated wavelength are
highlighted in grey.
Note: A black and an orange box are shown in the top left corner of
the display. The orange box relates to the reference scan and the
black one relates to the current wavelength scan.
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3. To complete the wavelength scan reading, refer to

• The newly plotted wavelength scan curves are shown in
black.
• The absorbance or transmittance value and the
associated wavelength are highlighted in black.
• In addition, the display shows the difference between the
wavelength scan curve and the reference curve against
the wavelength.
4. Press the black or orange small box in the left upper corner
on the screen to switch between the actual wavelength scan
and reference scan.
Second option:
1. Insert the zero solution cell into the cell compartment. Press
Zero.
2. Insert the analysis cell into the cell compartment. Press
Read.
• The newly plotted wavelength scan curves are shown in
black.
• The absorbance or transmittance value and the
associated wavelength are highlighted in black.
3. Press Options and then Reference: Off in the
"Options" menu to select another scan to display on the
same screen with the current scan. Highlight the required
scan number and press Highlight Reference.
Note: After selecting a reference scan the Reference: Off key in the
"Options" menu turns into Reference: On.
Note: Only scans that have the same wavelength range and step
can be displayed using the overlay option. This process can be
repeated until all matching scans are displayed.
4. The reference curve is shown in orange. The absorbance or

transmittance value and the associated wavelength are
highlighted in orange.
• In addition, the difference of the absorbance and/or
transmission value between the two scans (measured
scan and reference scan) is indicated/highlighted at each
position of the cursor.
Note: A black and an orange box are shown in the left corner of the
display. The orange box relates to the reference scan and the black
one relates to the current wavelength scan.
5. Press the black or orange small box in the left upper corner
on the screen to switch between the actual wavelength scan
and reference scan.
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6.7 Time course of absorbance/transmittance

The Time Course mode is used to collect data in either
absorbance or transmittance for a user-specified length of time.
This data can be displayed in either graphic or tabular format.
While the time scan is taking place, the cell compartment cover
must be closed. Therefore, a scan of the round cell compartment
is not possible.
6.7.1 Time course setup parameters

1. Press Time Course mode in the Main Menu.
2. Press Options to configure parameters.
Table 16 Time course options

Options Description
More For further options
Folder icon To store the scan data

To input the total time for data collection and the time interval between the collection of data
Time & Interval
points
 To input the wavelength setting
To display readings in absorbance, transmittance or concentration. This can be changed after
View Table
sample data is collected
Scale: In the automatic Scaling mode, the y-axis is automatically adjusted so that the total scan
is displayed. 
Scale & Units
The manual Scaling mode allows sections of the scan to be displayed. 
Units: Choice of absorbance or transmittance.
Send Data To send Data to a printer, computer or USB memory stick (Type A)
Recall measurement
data
Instrument Setup Basic data of the instrument, refer to Section 5.2, page 27.
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Time & Interval:

1. Press Time & Interval in the "Options" menu.
2. Enter the total time and measurement interval.
Note: In total, 500 measuring steps can be accomplished during a scan.
To select a total time and a time interval that would cause this number of
readings to be exceeded, the time interval is defined automatically and
the OK key is inactivated.
Scale & Units:

1. Press Scale & Units in the "Options" menu.
2. Highlight Abs or %T as the required units.
3. Highlight Auto or Manual scaling on the graph's y-axis.
Note: When selecting the manual scaling, the limit values y min and y max
can be entered using the alphanumeric keypad. The graph is adjusted to
display only the values in the selected range. If automatic scaling is
selected, the instrument sets the limits automatically so that the total
range can be displayed.
6.7.2 Time course scan reading
After all scan parameters have been selected, the instrument

must then be zeroed. Afterward the sample can be analyzed.
1. Insert the zero solution cell in the cell compartment and
close the cell compartment cover.
2. Press Zero.
The blank reading is shown on the display.
3. Insert the analysis cell in the cell compartment and close the
cell compartment cover.
4. Press Read.
The instrument will begin collecting Time Course data (kinetic
data).
Note: During the reading the Zero and Read keys change to Mark
and Stop.
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Expanded programs
• Select Mark to mark the next data point collected. This mark
is not used by the instrument, but is available for the user and
may indicate a significant event, such as the addition of a
sample or other reagent. The mark is also shown in the table.
• Select Stop to stop taking sample readings.
6.7.3 Analysis of time course data

After the Time Course data has been collected, the following
manipulations can be done on the graphic data:
The Time Course Program is complete, if

• the instruments beeps after completion of the overall time
• the graph is shown full-size,
• the scaling of the x-axis occurs automatically,
• the Cursor functions in the vertical navigation bar are
highlighted.
6.7.3.1 Navigation of a time scan or a time scan analysis

After a time scan has been completed, the time and the
absorbance/transmittance data is displayed as a curve.
Where the cursor is positioned on the curve, the elapsed time up
to this point and the corresponding absorbance are highlighted.
Table 17 Navigating the time scan

Cursor function/
Description
Zoom function
Delta mode: A second cursor is highlighted. The position of the fixed cursor was previously
defined in Cursor mode Single. Use the active cursor to select any point on the reading curve.
The difference to the fixed cursor is shown on the curve. The delta values are correspondingly
Curve Icon highlighted and displayed on the x and y axes.
(Choice of Cursor Mode) The gradient of the curve and the correlation coefficient (r2) between the cursor points in the
Delta mode are shown under the curve.
Cursor mode, single: The cursor moves to each selected measuring point of the scan.
The arrow keys (right/left) are used to move the cursor (depending on the selected mode) to the
next data point. The data of the data point (wavelength, absorbance or transmittance value) are
Arrow keys highlighted on the x and y axes.
Note: Press any point on the curve to display the associated data.
This function is used to magnify the section of the curve in the vicinity of the cursor. The original
Zoom Icon
curve size can be restored by pressing the zoom icon again.
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6.8 System Checks
1. Press System Checks in the Main Menu.
The "System Check" menu contains instrument information

and various performance tests.
6.8.1 Instrument Information
1. Press Instrument Information in the "System Check" menu.

2. The model, serial number and software version are
displayed.
6.8.2 Update the instrument software

To obtain the software for the update from the Internet at
www.hach.com.
1. Go to http://www.hach.com.
2. Select download resources>software downloads.
3. Enter DR 3900 in "Search for documents".
4. Locate the appropriate download file.
5. Open the ZIP file and save the files to the USB memory stick
or PC. The files have to be unzipped prior to saving on USB
or PC.
6. Press Instrument Update in the "System Check" menu.
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7. Connect the USB memory stick to the USB port (type A) on

the instrument, refer to Section 3.5, page 18.
The link is established automatically and the software is
updated.
Note: After the instrument update, a prompt to restart the instrument will
be displayed.
6.8.3 Optical Checks
1. Press Optical Checks in the "System Check" menu.

The "Optical Checks" menu contains programs for checking the
wavelength accuracy, stray light and photometric accuracy.
An optional test filter set (Verification Kit) Section 9, page 135()
containing six precision glass filters, target values and
instructions is available as an aid for carrying out comprehensive
in-house instrument checks.
6.8.3.1 Verification kit

The Verification Kit (refer to Section 9, page 135) is designed for
periodic monitoring of scattered light, photometric accuracy and
the wavelength accuracy of the spectrophotometers.
When results exceed allowable tolerances (given in the quality
control certificate to the test record), contact the manufacturer.
1. Press Verification Kit in the "Optical Checks" menu.

2. Press Nominal Values.
3. Press Edit.
An automatic menu guidance queries values (filters,
wavelength, nominal values and tolerances) given in the
quality control certificate, to the following specifications:
• Stray light
• Photometrical accuracy
• Wavelength accuracy
4. Press OK when all values are entered and the overview is
displayed.
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5. Press Verification.
6. Insert cell adapter A (Figure 3, page 19) in cell compartment
(2).
7. Remove any remaining cells from the cell compartment.

8. Close the cell compartment cover.
9. Press Start.
10. Follow the directions on the display and set various filters
one after another in the order displayed.
11. Press Next after inserting a filter.
After the last reading the results are displayed.

12. Press PC & Printer icon to send the data to a USB memory
stick, PC or to a printer.
The files will be stored automatically as CSV files (Comma
Separated Value). The file name will be formatted as
"Verification.csv".
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6.8.3.2 Wavelength check

The Wavelength Check test is used to check wavelength
accuracy at 807 nm.
1. Press Wavelength Check in the "Optical Checks" menu.

2. Insert the cell adapter (A) for 10-mm rectangular cells in the
cell compartment (2).
3. Place the analysis cell (Neodym or BG20/2) in the cell
adapter.
5. Press Start.
The result will be displayed.

6. Compare the result with the analysis cell required values.
7. Press Cancel to return to Optical Checks.
6.8.3.3 Stray light check

The Stray Light test is used to measure the stray light in the
instrument at 340 nm.
1. Press Stray Light Check in the "Optical Checks" menu.

2. Remove any cuvettes/analysis cuvettes from the cell
compartment.
3. Place the cell adapter (A) for 10-mm rectangular cells in the
cell compartment (2).
5. Press Zero.
6. Insert the analysis cell into the cell compartment (2).

8. Press Start.
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Expanded programs
The mean value is calculated from three consecutive

absorbance measurements. 
9. Compare the result with the required value from the analysis
cell.
6.8.3.4 Absorbance check
The Absorbance Check test is used to test the photometric

accuracy and repeatability of the instrument.
1. Press Absorbance Check in the "Optical Checks" menu.
2. Press  to input the wavelength.
4. Remove any cells from the cell compartment.
6. Press Zero.
7. Place the analysis cell in the cell compartment.

9. Press Read.
10. Remove the analysis cell.
12. Press Zero.
13. Place the analysis cell in the cell compartment.
15. Press Read.
16. Repeat this procedure until 5 comparison measurements
have been obtained.

17. Compare the result with the required value from the analysis
cell.
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6.8.4 AQA - Analytical Quality Assurance
1. Press AQA in the System Checks menu to configure AQA

measures or AQA in the toolbar to the right of the display, to
carry out AQA measures.
The AQA menu in System Checks contains the program for
checking the analytical quality. Configuration is done here,
for example the individual working method being controlled by
standard solutions or the cross-sensitivities of the analyses
identified by standard addition solutions, multiple determinations
and dilutions.
As optional accessories for full individual control, standard and
top-up solutions are available (Section 9, page 135).
6.8.4.1 AQA Standard Configuration
The working method, photometers, and accessories can be

checked by the analysis of a standard solution.
1. Press AQA Standard
A bar with standard sets opens (not available on all models),
alternatively an individual standard can be defined.
2. Select Options>New to define a new standard.
Note: If a standard has not yet been defined, in some models the
alphanumeric keypad is displayed directly.
3. Enter the name of the standard solution and confirm with

OK.
4. Enter the concentration unit and confidence interval by

touching the corresponding fields.
6. All the standard solutions entered are shown in the bar.

7. Select Options>Edit to alter the settings.
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8. Select Options>Delete to delete the standard. 

Select Options>New to define a new standard.
9. Select Options>Reminder to enter an automatic interval

when a standard analysis is required to be carried out.
10. Press On.
11. Select the required interval. Press OK to confirm the entry.
12. Select a unit for the interval. Press OK to confirm the entry.
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6.8.4.2 AQA Addition
By the analysis of a addition solution it is possible to check for

faults and interferences in the sample
1. Press AQA Addition.
2. Press On.
3. Enter the required interval, and the system will automatically

issue a prompt to add an analysis. Press OK to confirm the
entry.
4. Define the required unit for the interval. Press OK to confirm

the entry.
6.8.4.3 Multiple Determination
1. Press Multiple Determination.

2. Press Reminder <Off>.
3. Press On.
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6. Enter the number of measurements for multiple definitions.

7. Enter the tolerance as a percentage. Press OK to confirm.
8. Press OK to confirm all the entries.
6.8.4.4 Dilution
1. Press Dilution.
2. Press On.
5. Enter the dilution factor. Press OK to confirm.
6. Enter the tolerance as a percentage. Press OK to confirm.
7. Press OK to confirm all the entries.
6.8.4.5 Carring out AQA measures

If a reminder function has been set, once the set interval has
elapsed the requirement to carry out the AQA measure is issued.
1. Select the required AQA measure – for example a standard

measurement here.
2. Carry out the standard measurement via stored program or
barcode program.
3. An AQA symbol is displayed in the measurement window,
the AQA key in the toolbar is displayed as yellow.
4. The AQA measure is shown in a log as Passed or Failed.
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Expanded programs
6.8.4.6 Pipette test set

This function and the optional accessories tests the accuracy of
the pipette test set. To carry it out follow the instructions in the
pipette test set.
6.8.5 Instrument backup

Prior to the next service date, the "Instrument Backup" offers
the opportunity to store all programs, measurement data, user
IDs, passwords, as well as all adjustable data on a USB stick.
1. Press Instrument Backup in the "System Check" menu.

2. Insert the USB stick (refer to Section 3.5, page 18).
3. Press Store to start a Backup.
If the file was stored, the message "Instrument Backup is stored"

will be displayed.
Restore backup data:

Note: All current data will be overwritten when restoring the Backup file!
1. Press Instrument Backup in the "System Check" menu.

2. Insert the USB stick with the backup (refer to Section 3.5,
page 18).
3. Press Restore to pass back the data.
4. The report "Restore instrument backup from S/N XXXXXXX.

?" displays the version of the backup. Press OK to confirm.
5. After the backup start the instrument again.
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6.8.6 Service menu
Use of the service menu requires a service code. This menu is

reserved exclusively for servicing tasks.
6.8.7 Service time

In order to make sure a regular inspection is performed, an
automatic memory reference for the service times can be
entered. After switching the instrument on, this memory reference
will be activated and indicated at the appropriate time.
1. Press Service Time in the "System Check" menu.

2. Activate the On checkbox.
3. Press Last service.
4. Enter the date of the last service.
6. Activate the options field under Next service.

7. Select a time interval for the next service.
When the next service is due the report "Next service due!" is
displayed when the instrument is switched on.
9. Press OK to return to the Main Menu.
Contact the manufacturer to agree a date for the next service.
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Expanded programs
6.8.8 Lamps operating time

The "Lamps operating time" provides information on the
switch-on cycles of the lamps.
After a lamp is replaced, the display of the switch-on cycles is

reset to 0.
1. Press Lamp History in the "System Check" menu.
2. Press Reset and the lamp data are reset.
6.9 Toolbar
On the right of the display there is a toolbar with various

programs. Many of these programs can also be called up via a
menu but the operation is the same.
6.9.1 Log on
Use this function to enter up to 30 different operator initials (each

with a maximum of 10 characters) into the instrument. This
feature helps record which operator measured each sample. For
a detailed description, refer to Section 5.2, page 27.
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Expanded programs
6.9.2 Sample ID
Use this option to enter up to 100 different Sample Identification

tags (with up to 20 characters each) into the instrument. Sample
IDs can be used to specify the sample location or other sample
specific information, for example. For a detailed description, refer
to Section 5.2.2, page 29.
6.9.3 Timer
Use this function to activate a timer at any time.

1. In the toolbar, press Timer.
2. Enter the required time and confirm with OK.
The count-down starts automatically.

3. Press Close to let the timer run in the background.
Press Cancel to stop the timer.
4. When the timer has finished there is an acoustic signal.
6.9.4 AQA
The "AQA" menu contains the programs for testing the analytical
quality. For example, this controls the individual working method
with standard solutions or the cross-sensitivities of the analyses
identified by addition solutions, multiple determinations and
dilutions. For a detailed description, refer to Section 6.8.4,
page 116.
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Expanded programs
6.9.5 Trends
Use this function to call up a load curve of the stored
measurement values for each parameter or sample location. The
concentration of the corresponding parameter is shown
graphically over time. For a detailed description, refer to
Section 5.3.4.1, page 51.
6.9.6 Link2SC
Link2SC enables measurement values to be exchanged between
on-line process probes and the DR 3900. In this way on-line data
can be verified and process probes can be corrected.
The on-line data are sent to the DR 3900 in a "job file". After the
laboratory measurement values have been produced, the job file
is sent to the controller again for the corresponding probe to be
corrected.
Data transfer is either via memory card or network (LAN). For a
detailed description, refer to the Link2SC operating instructions.
6.9.7 Website DR 3900

Use this function to call up an Internet website on an existing
network connection. Current software updates, as well as
documentation and information on DR 3900 can be found here.
The labelling of the key varies from model to model and may vary
from the illustration in these instructions.
124
Section 7 Maintenance
CAUTION
Potential Chemical, Biological Eye and Skin Hazards.
Only qualified personnel should conduct the tasks described in this
section of the manual.
NOTICE
Remove all cells remaining in the instrument. Dispose of the cells or
their contents in an authorised manner.
7.1 Cleaning requirements
CAUTION
Potential Pinch, Eye, Burn and Chemical Hazards.
Before cleaning, always disconnect the instrument from the power
supply.
NOTICE
Never use solvents like turpentine, acetone or similar to clean the
instrument, including the display and accessories.
7.1.1 Spectrophotometer
• Only clean the housing, the cell compartments and all
accessories with a soft, damp cloth. A mild soap solution can
also be used.
• Do not get excess water in the cell compartments.
• Do not insert a brush or sharp object into cell compartment
#1 to avoid damaging the mechanical components.
• Dry the cleaned parts carefully with a soft cotton cloth.
7.1.2 Display
• Do not scratch the display. Do not touch the screen with ball
pens, pencils or similar pointed objects.
• Clean the display with a soft, lint-free and oil-free cotton cloth.
Diluted window cleaner liquid can also be used.
125
Maintenance
7.1.3 Cuvettes/cells
CAUTION
Potential Chemical/ Biological Exposure Hazards.
User correct laboratory practices if a risk of chemical exposure exists.
1. After use, clean the glass cells with cleaning agents.

2. Afterwards, rinse the cuvettes/sample cells several times
with tap water and then thoroughly with deionized water.
Note: Glass cuvettes/sample cells that have been used for organic
solvents (such as chloroform, benzene, toluene, etc.) must be rinsed
with acetone before being treated with cleaning agents. In addition,
another rinse with acetone is necessary as a final treatment step before
the cuvettes/sample cells are dried.
7.2 Lamp replacement
CAUTION
To avoid a possible electric shock, disconnect the instrument from the
power source before servicing the lamp.
1. Switch off instrument.

2. Remove the power cable from the instrument.
3. Turn the instrument to allow work access to the underside.
4. Using a slot-head screwdriver, undo the lamp cover screws.
5. Remove the cover.
NOTICE
Burn Hazard!
Wait until the lamp cools down. Contact with the hot lamp can cause
burns.
126
Maintenance
6. Using a cross-head screwdriver, remove the two screws for

the lamp holder.
7. Lift out the lamp holder upwards.

8. Turn the lamp holder to that the connector with the cable
guide points forwards.
9. Push the clamp slide as far as possible to the right (Step 1).
10. Grasp the connector and along with the lamp, pull it out of
the lamp holder (Step 2).
11. Release the connector from the lamp (Step 3).
127
Maintenance
Fig. 8 Lamp replacement
1 Lamp holder 3 Lamp

2 Clamp slide 4 Connector
To fit a new lamp carry out the procedure in reverse.
NOTICE
Only hold lamp at lamp holder. Avoid touching the glass, as
substances on the skin can bake onto the lamp bulb and thus
accelerate the ageing process of the lamp.
1. Connect a new lamp with the connector.

2. Replace the lamp along with the connector into the lamp
holder.
3. Secure the lamp with the clamp slide. To do this, push the
connector downwards and push the clamp slide as far as
possible to the left.
4. Replace the lamp holder into the instrument.
Note: Take care that the lamp cable is not pinched by the cable
guide.
5. Using a cross-head screwdriver, tighten the two lamp holder

screws.
6. Replace the lamp cover.
7. Using a slot-head screwdriver tighten the cover screws.
8. Connect the power cable to the instrument.
9. Reset the lamp operating time Section 6.8.8, page 122.
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Maintenance
7.3 Exchange of the replaceable cell compartment

If there is severe soiling, the complete cell compartment (2) can
be replaced.
1. Switch off instrument.

2. Remove the power cable from the instrument.
3. Using a cross-head screwdriver, undo the two screws on the
floor of the cell compartment.
4. Lift the cell receptacle upwards and out.

5. Emplace the new cell receptacle so that the pressure rollers
guide slot is to the left.
6. Using a cross-head screwdriver, tighten the two screws on
the floor of the cell compartment.
7. Connect the power cable to the instrument.
129
Maintenance
130
Section 8 Troubleshooting
Error displayed Definition Resolution
Attention! Readings with barcode cuvettes Insert the light shield.

Please insert the light shield. generally require the light shield. Press OK.
Reinsert the cell.
Barcode label not read Barcode faulty If the barcode is not recognised, contact
the manufacturer or his representative.
An error occurred when uploading Start the procedure again or contact the
the instrument data. manufacturer or his representative.
An error occurred when reading from Start the procedure again or contact the
the USB memory stick. manufacturer or his representative.
An error occurred when writing to the Start the procedure again or contact the
USB memory stick. manufacturer or his representative.
Please check on the current update

Error during update. Check the USB memory stick.
file.
Please contact Customer Services. Error during update.
Please check network configuration.
Please check the connection.
Please close the cover. Close the cover.

Insert a USB memory stick into a USB A
Please insert the USB memory stick.
port on the instrument.
Please check the connection and
Network setup or FTP error
contact the administrator.
Blank value correction not possible with
Blank value correction not possible!
LCW919.
File for instrument update missing. Error during update. Check the USB memory stick.
Save the update file again and repeat
File for instrument update is faulty. Error during update.
the procedure.
Switch the instrument of and then back
It’s recommended to execute a on again. If the system check is not
Check of the air values failed
Full System Check successful, contact the manufacturer or
his representative.
Forgotten your password?
Entry invalid! Password incorrect Contact the manufacturer or his
representative.
The measured absorbance exceeds
Absorbance > 3.5! Dilute sample and measure again
3.5
Fault
Barcode check number? Deviation to the stored data Data updating
Please update program data!
Error when calling up the local IP Network setup: DHCP client has no
Enter the IP address again.
address. connection to the DHCP server
Network setup: default gateway cannot
Error during default gateway setup. Try to create the connection again.
be set for fixed IP address
Error during network drive setup! Error during network setup Check the settings.
Network setup: Subnet mask cannot be
Error during subnet mask setup. Enter the subnet mask again.
set for fixed IP address
131
Troubleshooting
Error copying from USB memory Start the procedure again or contact the
Error during update
stick. manufacturer or his representative.
Make sure that the instrument is
Error in FTP connection. FTP error
connected to the network.
Fault
Program not accessible. Barcode test not present Data updating
Please update program data!
Fault The cuvette is soiled or there are Clean the cuvette; allow the particles to
Clean cuvette! undissolved particles in the cuvette settle
Fault Check the lamp and replace it if

Test program stopped! necessary.
Test program stops when the instrument
Please check lamp
is started Close lid.
Close the lid.
Error [xx] Press Start Again.
Fault Remove the cuvette/sample cell from

Test program stopped! Test program stops when the instrument the cell compartment.
Please remove the cuvette is started
Press OK.
Close the lid.
Error
Contact the manufacturer or a sales
Selfcheck stopped.
Electronic defect representative and indicate the error
Hardware error.
number
Error [x]
Error Decrease ambient light. (Avoid direct

Too much ambient light! The instrument sensors detects too sunlight.)
Move instrument into shade much ambient light.
Close lid.
or close the lid
No help function is available for this

program.
Shelf life exceeded!  The analysis is possibly erroneous. Use

Use chemicals? new chemicals
Check programming
Error in the test database / user
No evaluation! Contact the manufacturer or a sales
database
representative
Reinsert the cell.
No barcode! No barcode found If the barcode is not recognised, contact
the manufacturer or his representative.
No instrument backup present! Check the USB memory stick.
Data analysis not possible, no
No valid data for these parameters! Change the selection.
measurement data
No valid data found! View Data not possible in data log Change the selection.
No help function present.

Data analysis settings cannot be
No measurement data present! Change the selection.
configured without measurement data.
This is a warning notice. The control
Control range not reached! Data analysis limits not reached
limit set was not reached.
This is a warning notice. The control
Control range exceeded! Data analysis limits exceeded.
limit was exceeded.
Calculated concentration is higher than
Concentration too high! Dilute sample and measure again
999999
132
Troubleshooting

The measured absorbance is above the
Over measuring range Dilute sample and measure again
calibration range of the test
If possible, select a test with a lower
The measured absorbance is below the
Under measuring range reading range or use a cuvette with a
calibration range of the test
longer path length
The analysis is possibly erroneous due
Possible interference by: Interference Check
to interferences.
The analysis is possibly erroneous due
Possible interference from: Interference Check
to interferences.
Contact the manufacturer or his
representative for an inspection of the
Next service is due! instrument. For setting the service
reminder, refer to Section 6.8.7,
page 121
Negative result! The calculated result is negative Check concentration of sample
Network setup off, when access to Viper
Network switched off. Activate the online connection.
homepage via sidebar
Make sure that the instrument is
Remote server cannot be reached. Error during network setup
connected to the network.
Avoid direct sunlight at the measuring
Unstable lighting conditions!
location.
Insufficient memory for update . Error during update. Select a memory with more space.
Switch the instrument of and then back
on again. If the system check is not
System check incorrect! Measurement of air values failed
successful, contact the manufacturer or
his representative.
Switch of the instrument and allow it to
Temperature too high.
cool for a few minutes. If necessary,
Measurement not possible!
move it to a cooler place.
Save the update file again and repeat
Update file is faulty. Error during update.
the procedure.
USB memory stick is not connected. Update not possible. Check the USB memory stick.
Web server cannot be reached. Viper homepage cannot be reached. Try the connection again later.
133
Troubleshooting
134
Section 9 Replacement Parts
9.1 Accessories
Description Cat. No.

SIP 10 sipper set for DR 3900 with 1 inch round cell, EU LQV157.99.10001
RFID read/write device LOC 100: Transponder for location RFID, EU LQV156.99.10001
DR/check gel test standard 2763900
Verification kit LZV537
Thermal printer - Citizen PD 24, EU power cable 5835900.00
Thermal printer - Citizen 24, UK power cable 5835900.82
Thermal printer - Citizen 24, US power cable 296010
Thermal printer, Citizen PD 24, CH power cable 5835900.00
Paper for Citizen PD 24 LZM078
Paper for Citizen PD 24, US 5836000
Hand barcode scanner LZV566
USB-Memory Stick LZV568
Protective cap for USB port LZV881
USB extension cord LZV567
SD memory card LZV791
Reader for SD/MMC memory card, USB connection LZY522
Ethernet cable, shielded, 20 m in length. LZV873
Interface cable USB - computer XLH926
Cross-over cable LZX998
1 inch rectangular cells, matched pair, glass (10 ml) 2495402
1 cm rectangular cells, matched pair, glass (3.5 ml) 2095100
1 cm rectangular cell, quartz 2624410
1 inch round cell, glass, with cap (10 ml) 2122800
1 cm single use rectangular micro-cell (1.5 ml) 2629500
1 cm rectangular plastic cells 2743400
5 cm rectangular cell, glass (17.5 ml) 2629250
5 cm rectangular cell, quartz (17.5 ml) 2624450
1 cm rectangular flow cell, quartz, (450 µl) LZV510
5 cm rectangular flow cell, quartz (370 µl) LZV649
Stoppers, neoprene for 1 inch rectangular cells 1480801
9.2 Replacement Parts

Halogen lamp LZV565
Cell adapter A for 1-cm rectangular and 1-inch round cells LZV846
Cell adapter B for 3-cm rectangular cells, China only LZV847
Light shield LZV849
Bench top power supply LZV844
Power cord EU YAA080
Power cord CH XLH051
Power cord UK XLH057
135
Replacement Parts

Power cord US XLH055
Power cable China/Australia XLH069
Dust Cover LZV845
Cell compartment LZV848
136
Section 10 Contact
HACH Company Repair Service in the Repair Service in Canada: Repair Service in
World Headquarters United States: Hach Sales & Service Latin America, the
P.O. Box 389 HACH Company Canada Ltd. Caribbean, the Far East,
Loveland, Colorado Ames Service 1313 Border Street, Unit 34 Indian Subcontinent, Africa,
80539-0389 U.S.A. 100 Dayton Avenue Winnipeg, Manitoba Europe, or the Middle East:
Tel (800) 227-HACH Ames, Iowa 50010 R3H 0X4 Hach Company World
(800) -227-4224 Tel (800) 227-4224 Tel (800) 665-7635 Headquarters,
(U.S.A. only) (U.S.A. only) (Canada only) P.O. Box 389
Fax (970) 669-2932 Fax (515) 232-3835 Tel (204) 632-5598 Loveland, Colorado,
[email protected] Fax (204) 694-5134 80539-0389 U.S.A.
www.hach.com [email protected] Tel +001 (970) 669-3050
Fax +001 (970) 669-2932
[email protected]
HACH LANGE GMBH HACH LANGE LTD HACH LANGE LTD HACH LANGE GMBH
Willstätterstraße 11 Pacific Way Unit 1, Chestnut Road Hütteldorfer Str. 299/Top 6
D-40549 Düsseldorf Salford Western Industrial Estate A-1140 Wien
Tel. +49 (0)2 11 52 88-320 GB-Manchester, M50 1DL IRL-Dublin 12 Tel. +43 (0)1 912 16 92
Fax +49 (0)2 11 52 88-210 Tel. +44 (0)161 872 14 87 Tel. +353(0)1 460 2522 Fax +43 (0)1 912 16 92-99
[email protected] Fax +44 (0)161 848 73 24 Fax +353(0)1 450 9337 [email protected]
www.hach-lange.de [email protected] [email protected] www.hach-lange.at
www.hach-lange.co.uk www.hach-lange.ie
HACH LANGE GMBH HACH LANGE FRANCE HACH LANGE NV/SA DR. LANGE NEDERLAND
Rorschacherstrasse 30a S.A.S. Motstraat 54 B.V.
CH-9424 Rheineck 8, mail Barthélémy Thimonnier B-2800 Mechelen Laan van Westroijen 2a
Tel. +41 (0)848 55 66 99 Lognes Tel. +32 (0)15 42 35 00 NL-4003 AZ Tiel
Fax +41 (0)71 886 91 66 F-77437 Marne-La-Vallée Fax +32 (0)15 41 61 20 Tel. +31(0)344 63 11 30
[email protected] cedex 2 [email protected] Fax +31(0)344 63 11 50
www.hach-lange.ch Tél. +33 (0) 820 20 14 14 www.hach-lange.be [email protected]
Fax +33 (0)1 69 67 34 99 www.hach-lange.nl
[email protected]
www.hach-lange.fr
HACH LANGE APS HACH LANGE AB HACH LANGE S.R.L. HACH LANGE S.L.U.
Åkandevej 21 Vinthundsvägen 159A Via Riccione, 14 Edif. Arteaga Centrum
DK-2700 Brønshøj SE-128 62 Sköndal I-20156 Milano C/Larrauri, 1C- 2ª Pl.
Tel. +45 36 77 29 11 Tel. +46 (0)8 7 98 05 00 Tel. +39 02 39 23 14-1 E-48160 Derio/Vizcaya
Fax +45 36 77 49 11 Fax +46 (0)8 7 98 05 30 Fax +39 02 39 23 14-39 Tel. +34 94 657 33 88
[email protected] [email protected] [email protected] Fax +34 94 657 33 97
www.hach-lange.dk www.hach-lange.se www.hach-lange.it [email protected]
www.hach-lange.es
HACH LANGE LDA HACH LANGE SP. ZO.O. HACH LANGE S.R.O. HACH LANGE S.R.O.
Av. do Forte nº8 ul. Krakowska 119 Zastrčená 1278/8 Roľnícka 21
Fracção M PL-50-428 Wrocław CZ-141 00 Praha 4 - Chodov SK-831 07 Bratislava –
P-2790-072 Carnaxide Tel. +48 801 022 442 Tel. +420 272 12 45 45 Vajnory
Tel. +351 214 253 420 Fax +48 717 174 088 Fax +420 272 12 45 46 Tel. +421 (0)2 4820 9091
Fax +351 214 253 429 [email protected] [email protected] Fax +421 (0)2 4820 9093
[email protected] www.hach-lange.pl www.hach-lange.cz [email protected]
www.hach-lange.pt www.hach-lange.sk
HACH LANGE KFT. HACH LANGE S.R.L. HACH LANGE HACH LANGE SU
Vöröskereszt utca. 8-10. Str. Căminului nr. 3, 8, Kr. Sarafov str. ANALİZ SİSTEMLERİ
H-1222 Budapest XXII. ker. et. 1, ap. 1, Sector 2 BG-1164 Sofia LTD.ŞTİ.
Tel. +36 1 225 7783 RO-021741 Bucureşti Tel. +359 (0)2 963 44 54 Ilkbahar mah. Galip Erdem
Fax +36 1 225 7784 Tel. +40 (0) 21 205 30 03 Fax +359 (0)2 866 15 26 Cad. 616 Sok. No:9
[email protected] Fax +40 (0) 21 205 30 17 [email protected] TR-Oran-Çankaya/ANKARA
www.hach-lange.hu [email protected] www.hach-lange.bg Tel. +90312 4908300 Ext. 140
www.hach-lange.ro Fax +90312 4919903
www.hach-lange.com.tr
137
Contact
HACH LANGE D.O.O. ΗΑCH LANGE E.Π.Ε. HACH LANGE D.O.O. HACH LANGE MAROC
Fajfarjeva 15 Αυλίδος 27 Ivana Severa bb SARLAU
SI-1230 Domžale GR-115 27 Αθήνα HR-42 000 Varaždin Villa 14 – Rue 2 Casa
Tel. +386 (0)59 051 000 Τηλ. +30 210 7777038 Tel. +385 (0) 42 305 086 Plaisance
Fax +386 (0)59 051 010 Fax +30 210 7777976 Fax +385 (0) 42 305 087 Quartier Racine Extension
[email protected] [email protected] [email protected] MA-Casablanca 20000
www.hach-lange.si www.hach-lange.gr www.hach-lange.hr Tél. +212 (0)522 97 95 75
Fax +212 (0)522 36 89 34
www.hach-lange.ma
138
Section 11 Limited warranty
Hach Company warrants its products to the original purchaser
against any defects that are due to faulty material or
workmanship for a period of one year from date of shipment
unless otherwise noted in the product manual.
In the event that a defect is discovered during the warranty
period, Hach Company agrees that, at its option, it will repair or
replace the defective product or refund the purchase price
excluding original shipping and handling charges. Any product
repaired or replaced under this warranty will be warranted only for
the remainder of the original product warranty period.
This warranty does not apply to consumable products such as
chemical reagents; or consumable components of a product,
such as, but not limited to, lamps and tubing.
Contact Hach Company or your distributor to initiate warranty
support. Products may not be returned without authorization from
Hach Company.
Limitations
This warranty does not cover:
Damage caused by acts of God, natural disaster, labor unrest,
acts of war (declared or undeclared), terrorism, civil strife or acts
of any governmental jurisdiction
Damage caused by misuse, neglect, accident or improper
application or installation
• Damage caused by any repair or attempted repair not
authorized by Hach Company
• Any product not used in accordance with the instructions
furnished by Hach Company
• Freight charges to return merchandise to Hach Company
• Freight charges on expedited or express shipment of
warranted parts or product
• Travel fees associated with on-site warranty repair
This warranty contains the sole express warranty made by Hach
Company in connection with its products. All implied warranties,
including without limitation, the warranties of merchantability and
fitness for a particular purpose, are expressly disclaimed.
Some states within the United States do not allow the disclaimer
of implied warranties and if this is true in your state the above
limitation may not apply to you. This warranty gives you specific
rights, and you may also have other rights that vary from state to
state.
This warranty constitutes the final, complete, and exclusive
statement of warranty terms and no person is authorized to make
any other warranties or representations on behalf of Hach
Company.
139
Limited warranty
Limitation of Remedies
The remedies of repair, replacement or refund of purchase price
as stated above are the exclusive remedies for the breach of this
warranty. On the basis of strict liability or under any other legal
theory, in no event shall Hach Company be liable for any
incidental or consequential damages of any kind for breach of
warranty or negligence.
140
Appendix A Help Guide
The Help Guide is a step by step set of instructions for the
DR 3900, for the execution of a stored program or procedure in
accordance with process specifications. The Help Guide is
available for the most used HACH LANGE tests and the Hach
powder pillow reagent tests.
A.1 Display of the Help Guide for stored programs
1. Press Stored Programs in the Main Menu to view an

alphabetical list of stored programs with program numbers.
Highlight the required test and press Start.
2. Press the information icon.
3. The first procedure step according to the selected

test/method will be displayed.
Note: Use the arrow keys to navigate through the next or previous
pages or to the first or last page.
4. Follow the step-by-step descriptions on the screen.
5. If the method requires a timer, press Cancel and then press

the timer icon.
141
Help Guide
A list of preset times will be displayed.

6. Select the necessary time.
7. After the timer has finished, return to the Help Guide by
pressing the information icon.
8. The next help screen will be activated
9. Press Cancel to return to the reading screen of the
test/method.
10. Press the information icon to return to the last current page
of the Help Guide and proceed with the test.
A.2 Display of the Help Guide for barcode programs

1. Insert the light shield in cell compartment (2).
2. Press Barcode Programs in the Main Menu. Refer to
Section 5.5, page 64 for more information.
3. Press the information icon.
4. The list for barcodes will appear in increasing numerical

order.
5. Select the required test.
6. Prepare the barcode test in accordance with current process

specifications and follow the step by step instructions for the
procedure.
Note: Make sure that the values of the current process specification
are used.
Note: Use the arrow keys to navigate through the next or previous
pages or to the first or last page.
142
Help Guide
7. Press Cancel to return to the measurement screen for the

test.
8. Press the information icon to return to the last current page
of the Help Guide and complete the test.
The reading is started automatically and the results are

displayed.
143
Help Guide
144
Index
A E
Absorbance Check ................................................. 115 Editing Tests ............................................................. 62
Absorbance Formula .............................................. 100 Ethernet communication .......................................... 39
Alphanumeric keypad ............................................... 25 Ethernet port ............................................................ 18
AQA ....................................................................... 116
Audio signals ............................................................ 36 F
Factory Service ...................................................... 121
B Favorites ...................................................... 26, 63, 92
Barcode Programs ........................... 26, 64, 65, 66, 67 Favourites ................................................................ 91
editing ................................................................... 66 deleting ................................................................. 92
Basic data .......................................................... 56, 65 editing ................................................................... 91
Benchtop power supply ............................................ 15 recalling ................................................................ 92
Filter Settings ........................................................... 44
C Formula .............................................................. 67, 68
Calibration Settings .................................................. 78 Free programming .................................. 84, 90, 91, 92
Cell adapter .............................................................. 19
Cell compartment (1) ................................................ 18 H
Cell compartment (2) ................................................ 18 Help Guide ............................................................. 141
Cell compartment cover ........................................... 16
Chemical Form ................................................... 57, 59 I
Chemical form .................................................... 65, 66 Instrument Backup ................................................. 120
Cleaning Instrument Information ........................................... 111
Display ................................................................ 125 Instrument Setup ...... 26, 27, 57, 66, 98, 100, 103, 108
Spectrophotometer ............................................. 125 Integral ........................................................... 103, 104
Colour selection ....................................................... 43 Interfaces ........................................................... 18, 37
Concentration Factor ......................... 97, 98, 100, 101 Internet ............................................................. 71, 111
Concentration Resolution ................... 97, 98, 100, 102 IP address ................................................................ 39
Control bar ............................................................... 64
Cursor Mode .................................................. 103, 104 L
Cuvettes/cells ......................................................... 126 Lamp, refer to VIS lamp
Language ................................................................. 23
D Light shield ............................................................... 21
Data .......................................................................... 43 Link2SC .................................................................. 124
deleting ..................................................... 43, 46, 48 local networks .......................................................... 39
recalling .......................................................... 43, 48
sending ........................................................... 43, 48 M
storing ............................................................. 43, 48 Main menu ............................................................... 26
Data Folder .............................................................. 41 Maintenance ........................................................... 125
Data Log ................................................................... 43 Manual Update ......................................................... 62
Data log ...................................................................... 9 Marking .................................................................. 110
Data Storage ............................................................ 48 Measurement process .................................. 67, 68, 84
Date and time ........................................................... 35 Measuring sequence ................................................ 84
Default Gateway ...................................................... 40 Multi Wavelength ............ 26, 76, 78, 99, 100, 101, 102
Dilution Factor .............................................. 57, 58, 66
Display ....................................................... 25, 43, 125 N
Display and Sound ................................................... 36 Name ........................................................................ 67
Disposal ................................................................... 11 Network setup .......................................................... 39
Documentation overview ........................................ 124 Noise Check ........................................................... 114
Drift Check ............................................................. 122
145
Index
O S
Operating mode ......................................................... 9 Safety information .................................................... 11
Operator ID .............................................................. 27 Sample blank ........................................................... 67
creating ................................................................. 27 Sample ID ................................................................ 29
Optical Checks ............................... 112, 114, 115, 122 creating ................................................................. 29
Output Checks ....................................................... 122 deleting ................................................................. 30
Overview of Product/Function .................................. 13 Save as User Program ......................... 57, 66, 98, 100
sc controller ............................................................ 124
P Scale & Units .................................. 103, 105, 108, 109
Password ........................................................... 31, 43 Scanning speed ......................................................... 9
deactivate ............................................................. 34 Security List .............................................................. 31
PC and Printer ................................................... 37, 38 Select View ............................................................ 104
PC and printer .......................................................... 37 Self-Check ................................................................ 23
Photometric accuracy ................................................ 9 Send Data .................................... 45, 57, 66, 103, 108
Photometric linearity .................................................. 9 Server Name ............................................................ 41
Photometric measuring range .................................... 9 Service Time .......................................................... 121
Printer Setup ............................................................ 37 Single Wavelength ........................... 26, 75, 78, 97, 98
Printing data ............................................................. 38 Software ........................................................... 71, 111
Program overview .................................................... 26 Source lamp ............................................................... 9
Programming ..................................................... 71, 74 Specifications ............................................................. 9
Spectral bandwidth ..................................................... 9
R Standard Addition ............................................... 57, 93
Ratio ......................................................................... 53 Standard Adjust .................................................. 57, 59
Reagent Blank ............................................. 57, 60, 66 Startup melody ......................................................... 23
Recall Data ............... 49, 57, 66, 98, 99, 100, 103, 108 Startup process ........................................................ 23
Recall measurement data ........................................ 26 Stored Data .............................................................. 44
Reference Scan ............................................. 103, 106 Stored Programs ...... 26, 56, 57, 58, 59, 60, 62, 63, 66
Replaceable cell compartment ............................... 129 sending Data ......................................................... 57
RFID module ...................................................... 12, 16 Stray light ................................................................... 9
Stray Light Check ................................................... 114
Subnet Mask ............................................................ 40
System Check .................................................. 23, 121
System Checks ........ 26, 111, 114, 115, 120, 121, 122
T
Time & Interval ............................................... 108, 109
Time Course ......................................... 48, 49, 50, 108
Time course ............................................. 26, 108, 110
Time course scan reading ...................................... 109
Timer ............................ 36, 57, 58, 66, 67, 69, 97, 100
Toolbar ................................................................... 122
Touch screen ............................................................ 25
Troubleshooting ..................................................... 131
146
Index
U W
Unpack the instrument ............................................. 15 Wavelength ...................................................... 97, 100
Update ............................................................. 67, 111 Wavelength Accuracy ................................................ 9
Updating Tests ......................................................... 62 Wavelength calibration ............................................... 9
USB port type A ....................................................... 16 Wavelength Check ................................................. 114
USB port type B ....................................................... 17 Wavelength Range ................................................. 103
User Programs ......................................................... 74 Wavelength range ...................................................... 9
User programs ............................................... 9, 26, 73 Wavelength reproducibility ......................................... 9
Wavelength resolution ................................................ 9
V Wavelength Scan ........... 26, 48, 49, 50, 102, 103, 106
Variables ...................................................... 67, 69, 90 Wavelength selection ................................................. 9
Verification Kit ........................................................ 122 Wavelength setting ................................................. 108
Version ............................................................... 67, 68 Wavelength spectrum ............................................ 103
View Table .............................................................. 108
Viper-Web .............................................................. 124
VIS lamp
lamp control
VIS-Lamp
Lamp History ....................................................... 122
147
Index
148

User Manual 01/2011 Edition 1: © HACH Company, 2011. All Rights Reserved. Printed in Germany

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© HACH Company, 2011. All rights reserved. Printed in Germany.

Section 1 Specifications ........................................................................................................................ 9

5.2 Instrument Setup ...............................................................................................................................27

5.4 Stored Programs............................................................................................................................... 56

Section 6 Expanded programs.............................................................................................................73

6.8 System Checks............................................................................................................................... 111

Appendix A Help Guide ......................................................................................................................141

Operating mode Transmittance (%), Absorbance and Concentration

Source lamp Halogen lamp

Wavelength range 320–1100 nm

Wavelength Accuracy ± 1.5 nm (wavelength range 340–900 nm)

Wavelength reproducibility ± 0.1 nm

Wavelength calibration Automatic

Wavelength selection Automatic, based on method selection

Scanning speed  8 nm/s (in steps of 1 nm)

Photometric measuring range ± 3.0 Abs (wavelength range 340–900 nm)

User programs 100

Physical and environmental specifications

Width 350 mm (13.78 in)

Height 151 mm (5.94 in)

Depth 255 mm (10.04 in)

Mass 4200 g (9.26 lb)

Power connector via external power Input:100–240 V/50–60 Hz

Enclosure rating IP40 (excluding interfaces and power supply)

Protection class Class I

This product has been tested to the requirements of CAN/CSA-C22.2

2.1 Safety information

Note: Information that supplements points in the main text.

2.1.1 Precautionary labels

2.1.2 RFID module (not available on all models)

Follow the following safety information, in addition to any local

2.1.3 Chemical and Biological Safety

Normal operation of this instrument may involve the use of

• Observe all cautionary information printed on the original

2.2 Overview of product

3.1 Unpack the instrument

3.2 Operating environment

3.3 Front and back view

1 USB port type A 4 On/off switch

Figure 2 Back view

1 Ethernet port 3 USB port type A

3.4 Power connections

1. Connect the power cable to the benchtop power supply.

These USB ports enable data and graphics to be output to a

3.6 Cell compartments, cell adapters and light shield

Cell compartment (1) for:

Cell compartment (2) for:

Figure 3 Cell compartments

Figure 4 Cell adapter

Cell adapter A: 10-mm rectangular cells/1-inch round cell

3.6.2 Installation of cell adapter

Figure 5 Installation of a cell adapter

3.6.3 Use of the light shield for measurements

Figure 6 Light shield

Installation of the light shield

3.7 Beam path

Figure 7 Beam path

1 Halogen lamp 8 Splitter mirror