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    5the Bioreduction of The Ag

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    Afrah M
    The document describes characterization methods used to analyze silver nanoparticles (Ag-NPs) synthesized using Cinnamomum longa tuber powder. Specifically, it details: 1) UV-visible spectroscopy showed the Ag-NPs had a broad surface plasmon resonance peak at around 415nm, indicating homogeneous and fine nanoparticles. 2) X-ray diffraction and transmission electron microscopy showed the Ag-NPs had a spherical shape and small size in the range of 5-20nm. 3) The characterization methods confirmed the C. longa extract successfully reduced silver ions and stabilized the formed Ag-NPs via aldehyde groups being oxidized to carboxylic acids.

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    5the Bioreduction of The Ag

    Uploaded by

    Afrah M
    50 views2 pages
    The document describes characterization methods used to analyze silver nanoparticles (Ag-NPs) synthesized using Cinnamomum longa tuber powder. Specifically, it details: 1) UV-visible spectroscopy showed the Ag-NPs had a broad surface plasmon resonance peak at around 415nm, indicating homogeneous and fine nanoparticles. 2) X-ray diffraction and transmission electron microscopy showed the Ag-NPs had a spherical shape and small size in the range of 5-20nm. 3) The characterization methods confirmed the C. longa extract successfully reduced silver ions and stabilized the formed Ag-NPs via aldehyde groups being oxidized to carboxylic acids.

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    The document describes characterization methods used to analyze silver nanoparticles (Ag-NPs) synthesized using Cinnamomum longa tuber powder. Specifically, it details: 1) UV-visible spectroscopy showed the Ag-NPs had a broad surface plasmon resonance peak at around 415nm, indicating homogeneous and fine nanoparticles. 2) X-ray diffraction and transmission electron microscopy showed the Ag-NPs had a spherical shape and small size in the range of 5-20nm. 3) The characterization methods confirmed the C. longa extract successfully reduced silver ions and stabilized the formed Ag-NPs via aldehyde groups being oxidized to carboxylic acids.

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    © All Rights Reserved
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    Download as docx, pdf, or txt
    50 views2 pages

    5the Bioreduction of The Ag

    Uploaded by

    Afrah M
    The document describes characterization methods used to analyze silver nanoparticles (Ag-NPs) synthesized using Cinnamomum longa tuber powder. Specifically, it details: 1) UV-visible spectroscopy showed the Ag-NPs had a broad surface plasmon resonance peak at around 415nm, indicating homogeneous and fine nanoparticles. 2) X-ray diffraction and transmission electron microscopy showed the Ag-NPs had a spherical shape and small size in the range of 5-20nm. 3) The characterization methods confirmed the C. longa extract successfully reduced silver ions and stabilized the formed Ag-NPs via aldehyde groups being oxidized to carboxylic acids.

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    of 2

    Characterization methods and Ag(aq)  C. longa Stirring at room temp [Ag (C.

    longa)] (1)
    instruments
    The prepared Ag/C. longa were characterized by UV-
    visible
    Stirring for 24 h
    spectroscopy, X-ray diffraction (XRD), transmission
    [Ag (C. longa)]  R–CHOat [Ag (C.
    electron microscopy (TEM), scanning electron microscopy longa)]
       
    room tem p

    (SEM), energy dispersive X-ray fluorescence spectrometry  R–COOH


    (EDXRF) and Fourier-transform infrared (FT-IR) (2)
    spectroscopy. The
    UV-visible spectra were recorded over the 300–800 nm After dispersion of silver ions in the C. longa aqueous
    range with a UV-1650 PC UV-visible spectrophotometer solution matrix (Equation 1), the extract was reacted with the
    (Shimadzu, Osaka, Japan). The structures of the Ag-NPs Ag 
    (aq)
    to form [Ag (C. longa)] complex, which reacted with
    pro-
    duced were examined by XRD (XRD-6000; Shimadzu). aldehyde groups in the molecular structure of the
    The XRD patterns were recorded at a scan speed of methanolic extract to form [Ag (C. longa)], due to the
    4/minute. TEM observations were carried out on a H- reduction of silver ions through the oxidation of aldehyde
    7100 electron microscope (Hitachi, Tokyo, Japan), and the to carboxylic acid groups (Equation 2).
    particle-size distributions were determined using the
    UTHSCSA Image Tool version 3.00 program (freeware).
    SEM was performed using a Philips XL-30 instrument UV-visible spectroscopy analysis
    (Philips, Eindhoven, Netherlands) to study the The formation of Ag-NPs was followed by measuring the
    morphology of Ag/C. longa. The EDXRF was carried out SPR of the C. longa and Ag/C. longa emulsions over the
    on a DX-700HS spectrometer (Shimadzu). Meanwhile, wavelength range of 300–800 nm (Figure 3B). The SPR
    the FT-IR spectra were recorded over the range of 400– bands are influenced by the size, shape, morphology,
    4000 cm1 using an FT-IR Series 100, 1650 PerkinElmer composition, and dielectric environment of the prepared
    spectrophotometer (Los Angeles, CA). nanoparticles.25,26 Previous studies have shown that the
    spherical Ag-NPs con- tribute to the absorption bands at
    Results and discussion around 400–420 nm in the UV-visible spectra.26,27 These
    The reduction of Ag into Ag-NPs during exposure to absorption bands were assumed to correspond to the Ag-
    water extract of C. longa tuber powder was able to be NPs’ extra-fine nature, with relatively small size. UV-
    followed by the color change. The fresh suspension of C. visible absorption spectra (Figure 3B) showed that the
    longa was yellow. However, after the addition of AgNO3 broad SPR band contained one peak at 415 nm. This peak
    and stirring illustrates the presence of a homogeneous distribution of
    for 24 hours at room temperature, the emulsion turned hydrosol Ag-NPs after 24 hours stirring.11,23 For the
    brown. stability test of the Ag-NP emulsion, the absorption
    The color changes in aqueous solutions are due to the spectrum of the sample was measured after storage for 3
    surface-plasmon resonance (SPR) phenomenon (Figure 2A months (Figure 3C). The absorption peak of the Ag-NPs
    and B). The result obtained in this investigation is shifted slightly from 415 to 417 nm, but the spectra for
    interesting because it can serve as a foundation in terms of these two samples showed sig- nificant changes in either
    identification of potential forest plants for synthesizing peak intensity or spectral shape. Thus, with the
    27

    Ag-NPs. comparison of Figure 3B and C, it can be


    IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)

    DOI: 10.9790/3008-1303026875 www.iosrjournals.org 2 | Page

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