–CC– derived from heterocyclic compounds, eg, alkaloid 6. Zhang Y, Chen F, Zhuang J, et al.

Synthesis of silver nanoparticles

or flavones, and the amide (I) bond derived from the via electrochemical reduction on compact zeolite film modified
electrodes. Chem Commun (Camb). 2002:2814–2815.
proteins that are present in the tuber-powder extract are the 7. Szczepanowicz K, Stefan´ska J, Socha RP. Preparation of silver
capping ligands of the nanoparticles. 31 The broad and nanoparticles via chemical reduction and their antimicrobial activity.
Physicochem Probl Miner Process. 2010;45:85–98.
strong bands at 3329–2920 cm1 were due to bonded
8. Shameli K, Ahmad MB, Zargar M, Wan Yunus WMZ,
hydroxyl (–OH) or amine groups (–NH) and aliphatic C–H Rustaiyan A, Ibrahim NA. Synthesis of silver nanoparticles in mont-
of the C. longa tuber-powder extract, respectively. The morillonite and their antibacterial behavior. Int J Nanomedicine.
2010;6: 581–590.
peak at 1643 cm1 is attributed to the carboxyl group (– 9. Shameli K, Ahmad MB, Wan Yunus WMZ, Ibrahim NA. Synthesis
CO) stretching vibration. The adsorption at around 1375– and characterization of silver/talc nanocomposites using the wet
chemical reduction method. Int J Nanomedicine. 2010;5:743–751.
1420 cm1 notably showed that –NO324existed in residual
10. Shameli K, Ahmad MB, Zargar M, Wan Yunus WMZ,
amounts. The broad peaks at 510 and 299 cm1 and also the Ibrahim NA. Fabrication of silver nanoparticles doped in the zeolite
peak in 1952 cm1 are related to Ag-NP banding with framework and antibacterial activity. Int J Nanomedicine. 2011;6:
331–341.
oxygen from hydroxyl groups of 11. Shameli K, Ahmad MB, Jazayeri SD, et al. Synthesis and
C. longa compounds (Figure 7B).27 characteriza- tion of polyethylene glycol mediated silver
nanoparticles by the green method. Int J Mol Sci. 2012;13:6639–
6650.
Conclusion 12. Pastoriza-Santos I, Liz-Marzán LM. Formation and stabilization of
Ag-NPs with an average size of 6.30  2.64 nm and silver nanoparticles through reduction by N,N-dimethylformamide.
Langmuir. 1999;15:948–951.
spherical shapes were synthesized using aqueous tuber-
13. Ahmad MB, Shameli K, Wan Yunus WMZ, Ibrahim NA. Synthesis
powder extract of C. longa. The Ag-NPs were and characterization of silver/clay/starch bionanocomposites by
characterized by UV-visible, XRD, TEM, SEM, EDXRF, green method. Aust J Basic Appl Sci. 2010;4:2158–2165.
14. Setua P, Pramanik R, Sarkar S. Synthesis of silver nanoparticle inside
and FT-IR spectra. Biosynthesis of Ag-NPs using green the nonaqueous ethylene glycol reverse micelle and a comparative
resources like C. longa is a better alternative to chemical study to show the effect of the nanoparticle on the reverse micellar
aggregates through solvation dynamics and rotational relaxation
synthesis, since this green synthesis is pollutant-free and measurements. J Phys Chem B. 2010;114:7557–7564.
eco-friendly. From the results obtained in this research, 15. Praus P, Turicová M, Klementová M. Preparation of silver-
one can affirm that C. longa tuber powder can play an montmorillonite nanocomposites by reduction with formaldehyde
and borohydride. J Braz Chem Soc. 2009;20:1351–1357.
important role in the bioreduction and stabiliza- tion of 16. Sun L, Zhang Z, Dang H. A novel method for preparation of silver
silver ions to Ag-NPs. nanoparticles. Mater Lett. 2003;57:3874–3879.
17. Raveendran P, Fu J, Wallen SL. Completely “green” synthesis and
stabilization of metal nanoparticles. J Am Chem Soc. 2003;125:
Acknowledgments 13940–13941.
The authors thank the University Putra Malaysia (UPM) 18. Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS.
Cinnamon zeylanicum bark extract and powder mediated green
for its financial support (RUGS, Project no 9199840). The
synthesis of nano-crystalline silver particles and its bactericidal
authors are also grateful to the staff of the Department of activity. Colloid Surface B. 2009;73:332–338.
Chemistry, UPM, for their help in this research, and the 19. Rai A, Singh A, Ahmad A, Sastry M. Role of halide ions and
tempera- ture on the morphology of biologically synthesized gold
Institute of Bioscience (IBS/UPM) for technical nanotriangles. Langmuir. 2006;22:736–741.
assistance. 20. Gardea-Torresdey JL, Parsons JG, Dokken K, et al. Formation and
growth of Au nanoparticles inside live alfalfa plants. Nano Lett.
2002;2:397–401.
Disclosure 21. Gardea-Torresdey JL, Gomez E, Peralta-Videa JR, Parsons JG,
The authors report no conflicts of interest in this work. Troiani H, Jose-Yacaman M. Alfalfa sprouts: a natural source for the
synthesis of silver nanoparticles. Langmuir. 2003;19:1357–1361.
22. Shankar SS, Rai A, Ankamwar B, Singh A, Ahmad A, Sastry M.
References Biological synthesis of triangular gold nanoprisms. Nat Mater.
1. Mohanpuria P, Rana NK, Yadav SK. Biosynthesis of nanoparticles: 2004;3: 482–488.
technological concepts and future applications. J Nanopart Res. 2008;10: 23. Zargar M, Hamid AA, Bakar FA, et al. Green synthesis and antibacte-
507–517. rial effect of silver nanoparticles using Vitex negundo L. Molecules.
2. Mohamed MB, Volkov V, Link S, Sayed MAE. The ‘lightning’ gold 2011;16:6667–6676.
nanorods: fluorescence enhancement of over a million compared to the 24. Shameli K, Ahmad MB, Al-Mulla EAJ, et al. Green biosynthesis of
gold metal. Chem Phys Lett. 2000;317:517–523. silver nanoparticles using Callicarpa maingayi stem bark extraction.
3. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of Molecules. 2012;17:8506–8517.
antimicrobials. Biotechnol Adv. 2009;27:76–83. 25. Kelly KL, Coronado E, Zhao LL, Schatz GC. The optical properties
4. Shameli K, Ahmad MB, Wan Yunus WMZ, Ibrahim NA, Gharayebi of metal nanoparticles: the influence of size, shape and dielectric
Y, Sedaghat S. Synthesis of silver/montmorillonite nanocomposites environment. J Phys Chem B. 2003;107:668–677.
using 26. Stepanov AL. Optical properties of metal nanoparticles synthesized
-irradiation. Int J Nanomedicine. 2010;5:1067–1077. in a polymer by ion implantation: a review. Tech Phys. 1997;49:
5. Shameli K, Ahmad MB, Wan Yunus WMZ, et al. Green synthesis 143–153.
of silver/montmorillonite/chitosan bionanocomposites using the
UV-irradiation method and evaluation of antibacterial activity. Int J
Nanomedicine. 2010;5:875–887.
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