Name: Virtual Microscope by BIONETWORK: Online Edition

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The passage describes how to use a virtual microscope and identifies different parts of plant and blood cells when viewed under different magnifications.

The eyepiece/ocular lens, nosepiece, objectives lenses, stage, diaphragm, coarse and fine adjustment knobs were identified along with their functions.

Plant cells are boxy with cell walls, chloroplasts, and nuclei while blood cells are round and smaller without cell walls. Plant cells are green while blood cells are violet.

Name:

Virtual Microscope by BIONETWORK ONLINE EDITION http://www.ncbionetwork.org/iet/microscope/

Instructions: You can use the “learn” tab to familiarize yourself with the virtual microscope. As you click on each of the
microscope parts, read the pop-up to answer the following.

1. Identify each of the following by letter.

K On/Off Switch C Eyepiece / Ocular Lens

J Arm F Nosepiece

G Objective Lenses D Stage

H Diaphragm E Stage Adjustment

M Base L Coarse Adjustment

A Lens Paper N Fine Adjustment

B Immersion Oil P Slide box

O Kimwipes

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2. Explain what each of the following does:

kimwipes Clean sensitive electronic components

immersion oil Concentrates path of light on lens in order to increase resolution of image

diaphragm Adjusts amount of light passing through stage opening


stage adjustment knob Moves the slide forward, back, left, and right

Go to the “EXPLORE” tab to view slides. You will need to use the coarse and fine focus and adjust the light to get clear
views of the following. The slide can also be moved within the viewing field.

Sample Slides - Letter E

3. Include a screenshot of the letter “e” at 10x. To take a screenshot, use the print screen on your keyboard or use your
phone to capture the screen. Insert the image in the space below. Your image should clearly show that you viewed the
“e” and focused it.

4. What did you need to do before viewing the slide at 100x?

Went to 40x in order to prevent damaging the microscope and ensure as accurate of focus as
possible, and then add immersion oil to the 100x lens

5. A common mistake for beginners is thinking that the “e” just disappears at the high magnification. How would you
explain to a younger student what happened to the e as you increase the magnification?

You're viewing it as if it was 10x - 100x bigger and then you can only see a small portion of what you want to see
which gives the illusion of it disappearing

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Plant Cells

Return to the slide box and choose plant slides → plant cells. Focus with 4x, 10x, and 40x. You may need to adjust the

light and center your slide.

6. Include a screenshot of the plant cells at 40x.

7. Plant cells can be identified by their boxy appearance, where lines represent cell walls. Within the cell are green
circles which are chloroplasts and a dark gray area which represents the nucleus.

At 40x, how many individual cells can be seen in the viewing field?
5

Human Blood

Return to the slide box and choose human → blood Focus with 4x, 10x, and 40x. You may need to adjust the light and

center your slide.

8. Include a screenshot of the blood cells at 40x.

9. Blood cells can be identified by their round appearance. Unlike plant cells, they do not have cell walls.
Around
At 40x, how many individual blood cells can be seen in the viewing field?
100
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10. At 100x, you will see a cell that is larger and has a dark shape in it. This cell doesn’t look like the others because it is
a white blood cell and it functions to protect your body against microbes. Red blood cells do not have a nucleus. White
blood cell count is used as a diagnostic tool to determine if someone is sick. If you were fighting an infection, would you
expect to have more or less WBC’s? Why?

You would have less because they have to go and fight the infection which will cause them to "die." Afterwards you'll
have a lower count and soon your wbc count will be back to normal

Synthesis and Discussion

11. Compare blood cells to plant cells, include details about color, general shape, and size. Write this as a complete
sentence or paragraph.

Plant cells and blood cells are very different, there are many more blood cells in a small sample than
plant and they are much smaller too, while plant cells are green, blood is an interesting violet color

12. Explore bacteria slides. You don’t need to screenshot them. Compare the bacteria slides to the blood cells and
include details about their color, general shape, and size. Write this as a complete sentence or paragraph.

The acid fast mix is an odd shade of blue, and it is very spread out and unqiquely shaped, while blood is violet and
always stays a circle and stays bunched up

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