PCR Thermal Cycler

Best solution for your DNA amplification needs

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PCR Thermal Cycler

PCR Thermal Cycler Products

Welcome to Esco 03
Esco Global Network 04
Thermal Cycler Products Overview 05

Aeris™ Thermal Cycler 06

Aeris™ Thermal Cycler General Specifications 07
Aeris™ Thermal Cycler Ordering Information 07
Aeris™ Thermal Cycler Key Features 08

Swift™ MiniPro® Thermal Cycler 12

Swift™ MiniPro® Thermal Cycler General Specifications 13
Swift™ MiniPro® Thermal Cycler Ordering Information 13

Swift™ Spectrum 48 Real-Time PCR Detection System 14

Swift™ Spectrum 48 Real-Time PCR Detection System General Specifications 15
Swift™ Spectrum 48 Real-Time PCR Detection System Ordering Information 15
Swift™ Spectrum 48 Real-Time PCR Detection System Key Features 16

Swift™ Spectrum 96 Real-Time PCR Detection System 18

Swift™ Spectrum 96 Real-Time PCR Detection System General Specifications 19
Swift™ Spectrum 96 Real-Time PCR Detection System Ordering Information 19
Swift™ Spectrum 96 Real-Time PCR Detection System Key Features 20

Provocell™ Shaking Micro Incubator 22

Provocell™ Shaking Micro Incubator General Specifications 23
Provocell™ Shaking Micro Incubator Ordering Information 23
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PCR Thermal Cycler

WELCOME TO ESCO
Esco’s vision is to provide enabling technologies for scientific discoveries to make human lives healthier and safer.
Esco represents innovation and forward-thinking designs, which are all coupled with the highest standard quality since 1978. The Esco Group of
Companies remains dedicated in delivering innovative solutions for the clinical, life sciences, research, industrial, laboratory, pharmaceutical and IVF
community. With the most extensive product line in the industry, our products have passed a number of international standards and certifications.
Esco operates under ISO 9001, ISO 14001 and ISO 13485.

Availability and Accessibility

Headquartered in Singapore, manufacturing facilities are located in Asia and Europe. R&D is conducted worldwide spanning the US, Europe
and Asia. Sales, services and marketing subsidiaries are located in 12 major markets including the US, UK, Japan China and India. Our regional
distribution centers are located in China, UK, India, Malaysia, Philippines, Singapore, South Africa, South Korea and United States of America.
Because of our worldwide presence, you can be sure that Esco is within your reach.

High Quality, Reliable and Dependable

Our customers are confident that only with the best quality, reliable and dependable products, can they be sure of the accuracy of their research
and procedures. Cross functional teams from Esco Production, R&D, Quality Assurance and Senior Management, are regularly assembled to review
and implement areas for improvement.

Esco Cares for Your Safety

Esco focus on providing safety not just for your samples but also for users.

Esco Cares for Your Comfort

Comfort of our users is ensured by building ergonomic designs and by reducing the noise levels of the units.

Esco Cares for the Environment

One in every four of Esco’s employees is involved in Research and Development and a number of these evaluate new components and/or designs
to produce energy efficient equipment. Being GREEN is more than just modifying the parts we use to produce a new energy efficient technology,
it also embodies the every aspect of our company.

Customer Service and Support

Our service does not stop once purchase has been made. Esco gives on-time customer service and offers end-user seminars, service training, and
preventive maintenance, provides educational materials and informative videos.

As Esco takes the opportunity to respond to the world’s needs, we aim not just to contribute in the advancement of scientific discoveries but also
in making the world a safer, healthier and a better place to live in.
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PCR Thermal Cycler

global network

Thermal Cycler Products Overview

Aeris™ Thermal Cyclers Swift™ Series Thermal Cyclers, MiniPro ®

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PCR Thermal Cycler

Global Offices
Distributors
Factories
R&D Centers
Regional Distribution Centers

Swift™ Series Thermal Cyclers, Provocell™ Shaking Micro Incubator

Spectrum 96 Real-Time
PCR Detection System
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Aeris™ Conventional PCR Thermal Cycler

Aeris™
Conventional PCR
Thermal Cyclers

Aeris™ Thermal Cyclers

The Aeris™ thermal cycler offers five interchangeable blocks designed to meet critical requirements for different applications.
It comes with an intuitive touch screen to deliver easy-to-use programming; AeonStar™ Peltier is qualified to deliver
outstanding and precise performance and unique IsoHeat™ temperature control technology delivering high heating and
cooling rates with excellent temperature accuracy and uniformity. SmartDrive™ automatic block recognition increases user
convenience. AerisLine™ software enables the remote control of up to 30 individual units via one PC.

Additional Features
• Multi-block capability with automatic block recognition software minimizes the need for manual settings
• Adjustable hot lid temperature and ramp rate
• Powerful software that meets a variety of experimental requirements, such as Touchdown PCR, Time Release PCR, In Situ
PCR, and others
• The Peltier module, electronics, and sensors are precision tuned and tested to ensure the longest operating lifespan possible
• Pre-programmed methods provide easy choice
• Large internal memory that can store up to 250 individual protocols and unlimited data using USB memory stick or PC
• Password protection guarantees secure system access
• AerisLine™ allows you to control up to 30 Aeris™ thermal cyclers via one PC
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Aeris™ Conventional PCR Thermal Cycler

General Specifications, Aeris™ Thermal Cycler

Model Code AERIS-BG096 AERIS-B4830 AERIS-BG384 AERIS-BD048 AERIS-B4076
Sample Capacity 96 x 0.2 ml 48 x 0.2 ml + 30 x 0.5ml 384 wells 48 x 0.2 ml + 48 x 0.2 ml 4 slides in situ

0.2 ml tubes
0.2 ml tubes
96-well microplates 0.2 ml tubes
Application Consumables 12 x 8 strips
0.5 ml tubes 384-well microplates
6 x 8 strips
4 slides in situ
4 x 12 strips
8 x 12 strips

Maximum Heating Rate 4.0ºC/sec 2.8ºC/sec 2.8ºC/sec 4.0ºC/sec 1.8ºC/sec

Maximum Cooling Rate 4.0ºC/sec 2.8ºC/sec 2.8ºC/sec 4.0ºC/sec 1.8ºC/sec

Temperature Control Mode Tube or Block

Temperature Range 4-105ºC

Over-temperature Cut-Out Yes

Number of Programs Up to 250 programs, unlimited with USB flash drive

Maximum Hold Time 59 min and 58 sec

Temperature Accuracy ≤±0.1ºC below 50ºC

Temperature Uniformity ≤±0.2ºC below 55ºC

Hot Lid Temperature Range 30-110°C (Adjustable, Default 105°C, Automatic Hot-Lid)

PCR Sample Volume 10-100 μl

Tm Calculator Auto

Extensive Experiment Option setting for time up/down is between 0-9 min 59 sec, which is suitable for Long PCR Temperature when up/down is
Application between 0.1ºC to 9.9ºC, it is suitable for Touchdown PCR

Auto Re-start on Power Failure Yes

Connection to PC Control Yes

Software AerisLineTM

Operation System Windows XP / Windows Vista / Windows 7 / Windows 8

Pre-Run Sample Cooling Yes, 4ºC

Language English, Chinese, Spanish

USB Yes

Display 6.5“ Color LCD Touch Screen

Dimensions (W x D x H) 306 x 386 x 295 mm (12.0“ x 15.2“ x 11.6“)

Power Supply, Consumption 100-240 VAC, 50/60 Hz, 600 W

Warranty 3 years for mainbody, 2 years for blocks

Net Weight 9 Kg (19.8 lbs) (without block)

Shipping Weight 10 Kg (22.0 lbs)

Shipping Dimension 420 x 540 x 370 mm

(W x D x H) (16.5’’ x 21.3’’ x 14.6’’)

*The parameters are tested under optimized lab environments.

Ordering information
Ordering Information, Aeris™ Thermal Cycler

Model Code Item Code Description

AERIS-MB 2210003 Aeris™ Thermal Cycler Main Body (100-240 VAC)

AERIS-BG096 2210004 Aeris™ Thermal Cycler Block (96 x 0.2 ml)

AERIS-B4830 2210005 Aeris™ Thermal Cycler Combined Block (48 x 0.2 ml + 30 x 0.5 ml)

AERIS-BG384 2210006 Aeris™ Thermal Cycler Block (384 wells)

AERIS-BD048 2210007 Aeris™ Thermal Cycler Dual Block (48 x 0.2 ml)

AERIS-B4076 2210008 Aeris™ Thermal Cycler (4 slides in situ)

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Aeris™ Conventional PCR Thermal Cycler

Flexible - Your Application, Your Cycler

Five Interchangeable Blocks

AERIS-BG096
G-96 WELL
Applicable consumables: 0.2 ml tube,
AERIS-BG384
96-well microplate, 12 x 8 strips, 8 x 12
G-384 WELL
strips
Applicable consumables: 384-well microplate

AERIS-B4830
48 x 0.2 ml + 30 x 0.5 ml WELL
Applicable consumables: 0.2 ml tubes,
0.5 ml tubes, 4 x 12 strips
AERIS-B4076
4 IN SITU SLIDES
For In Situ PCR

Applicable consumables: 4 slides in situ

AERIS-BD048
D-48 X 0.2 ml
Two in one! Two independent experiments may be
carried out at the same time.

Applicable consumables: 0.2 ml tubes, 6 x 8 strips

Easier Programming

Main Interface New Protocol

Shortcut Run
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Aeris™ Conventional PCR Thermal Cycler

wider application
Why Use Nested PCR?
Nested PCR is a modification of a polymerase chain reaction technique
intended to reduce PCR product contamination due to the amplification of
non-specific primer
binding sites.

Why Use Long PCR?

Long PCR, a new technique based on ordinary PCR, applied to amplify the PCR
template longer than 5 Kb.

Why Use Touchdown PCR?

Touchdown PCR is a method of polymerase chain reaction by which primers
avoid amplifying non-specific sequences. The annealing temperature during a
polymerase chain reaction determines the specificity of primer annealing. The
melting point of the primer sets the upper limit on annealing temperature. At
temperatures just below this point, only very specific base pairing between the
primer and the template occurs.

End Point Analysis Result

The best conditions are found in Well 10, where the temperature
was 63.2ºC.
Note: Experimental determination of optimal annealing
temperature. The calculated primer annealing temperature was
56.5°C. The actual annealing temperature was 63.2°C.
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Aeris™ Conventional PCR Thermal Cycler

AerisLineTM PC Software
Easy Setup
Network Enabler Administrator helps you configure the instrument
by IP address.

Simple
Once you install the software, you get easy access to set up
protocols and edit the program.

Powerful
One PC can control up to 30 Aeris™ Thermal Cyclers.

tm calculator
Tm calculator allows you to calculate the optimal PCR
annealing temperature based on the sequence of a pair
of primers. The Tm calculator by default calculates by the
simpler GC content.

Three modes of operation

Stand-Alone Unit
1 Operate directly with keypad.

2 PC Controlled
Operate cycler via PC, and save programs.

Satellite Function Via AerisLine™

3 Up to 30 Aeris cyclers can be controlled from
one PC.
30x
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Aeris™ Conventional PCR Thermal Cycler

Aeristm components
USB Port Touch Pen

Black Cable White Cable Fuse

For single unit connecting to For multiple units connecting to

AerisLineTM PC software AerisLineTM PC software

USB port
• User friendly
• Convenient and quick data transfer
• Convenient USB port and RJ45 port simplify data transfer and product updates between the Aeris™ Thermal Cycler
and USB memory stick
Storage RJ45
There are up to 250 Aeris™ Thermal Cycler and
protocols that can be saved PC / Laptop (update
in the internal memory; software via RJ45 port when
unlimited with use of USB enhancements are available)
memory stick and PC. Save
as many of your commonly
used programs as you want

AERIs™ Benefits
• Saves time when programming with the intuitive color touch screen

• Keeps the latest operation records which deliver the

proven reliability of PCR results

• Durable design to guarantee longer instrument lifespan

• Tm calculator for optimized primer annealing temperature

• Flexibility for extensive applications such as Long PCR and

Nested PCR

• Hot lid temperature adjustment to secure the temperature control

on the block and to prevent condensation and water evaporation
on the hot lid itself

• Better performance with temperature accuracy

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Swift™ MiniPro® Conventional PCR Thermal Cycler

Swift™MiniPro ®

Conventional PCR
Thermal Cyclers

Swift™ Series Thermal Cyclers, MiniPro®

The Esco Swift™ MiniPro® thermal cycler is a low cost personal thermal cycler with a compact footprint, suitable for a variety
of critical experimental applications, such as Touch Down PCR, Time Release PCR and others. The Swift™ MiniPro® thermal
cycler uses advanced peltier technology to achieve precise temperature control and fast ramp rates with minimal over- and
under-shoot for process speed and accuracy.

Superior Performance
Excellent Temperature Uniformity Convenient Setup, Fast Run
Unique IsoHeat™ temperature control technology guarantees Pre-programmed methods are available for your convenience
extremely uniform temperature between central and edge or you can enter thermal cycling values to program your own
methods.
wells. Block temperature uniformity is <0.3°C.

Friendly Interface
High Temperature Precision
Large screen shows all information in one page. Easy,
Precisely tuned and tested AeonStar™ peltier, temperature graphical programming for temperature, holding time, ramp
sensor, and proprietary control algorithms provide highest
rate, pause and other functions ensures intuitive operation.
temperature accuracy. Block temp. accuracy is <0.3°C.
Adjustable Hot Lid
Outstanding Ramp Rate Prevents reagents from evaporating. Hot lid height is
Proprietary aluminium block with superior thermal adjustable to suit all kinds of tubes.
conductivity properties delivers superb heating and cooling
performance, equal to the gold blocks of other brands. High
ramp rate of up to 5.0°C / sec. Adjustable Ramp Rate
High ramp rate of up to 5.0°C / sec. Suits all reagents. Allows
protocols to be transferred from other cyclers.

Stability
Robust Security
Automatic restart saves setpoints and guarantees successful
Protocol Setting Display PCR Running Display
PCR cycling in the event of power interruption.
Long Warranty Period, Peace of Mind
Convenience The peltier module, electronics and sensors are precision
tuned and tested to ensure the longest operating lifespan
Compact Footprint possible. Backed by an industry leading 3 year warranty for
User-friendly ergonomic design, small footprint to conserve main body, 2 year warranty for block.
available bench top space. Lightweight - only 3.5 Kg (7.7 lbs).
ISO 9001:2000 Certificate
Certified Quality CE & EMC Certificate
ISO 14001:2004 Certificate
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Swift™ Spectrum 48 Real-Time PCR Detection System

General Specifications, Swift™ MiniPro® Thermal Cycler

Model Code SWT-MIP-0.2-_ SWT-MIP-0.5-_

Sample Capacity 24 x 0.2 ml 18 X 0.5 ml

Applicable Consumables 0.2 ml tubes, 3 X 8 strips, 24-well microplates 0.5 ml tubes

Temperature Range 4-99°C

Maximum Heating Rate 5.0°C / sec 4.0°C / sec

Maximum Cooling Rate 4.0°C / sec 3.0°C / sec

Temperature Uniformity ±0.3°C

Temperature Accuracy ±0.3°C

Hot Lid Temperature Range 30-110°C (Adjustable, Default 105°C, Automatic Hot-Lid)

PCR Sample Volume 10-100 μl

Temperature Control Mode Tube or Block

Display Graphical LCD

Protocol Capacity 100 protocols

PC Interface RS232 for software updates

Dimension (W x D x H) 212 × 297 × 200 mm (8.3” × 11.7” × 7.9”)

Power supply, Consumption 100-120 VAC / 200-240 VAC, 50/60 Hz, 200 W

Warranty 3 years for main body, 2 years for blocks

Net Weight 3.5 Kg (7.7 lbs)

Shipping Weight 4.5 Kg (9.9 lbs)

Shipping Dimensions 320 x 420 x 330 mm

(W x D x H) (12.6’’ x 16.5’’ x 13.0’’)

Ordering information
Order Information, Swift™ MiniPro® Thermal Cycler

Model Code Item Code Description

SWT-MIP-0.2-1 2210009 Swift™ MiniPro® Thermal Cycler With 24 x 0.2 ml Block 110 VAC 50/60 Hz

SWT-MIP-0.2-2 2210010 Swift™ MiniPro® Thermal Cycler With 24 x 0.2 ml Block 220 VAC 50/60 Hz

SWT-MIP-0.5-1 2210011 Swift™ MiniPro® Thermal Cycler With 18 x 0.5 ml Block 110 VAC 50/60 Hz

SWT-MIP-0.5-2 2210012 Swift™ MiniPro® Thermal Cycler With 18 x 0.5 ml Block 220 VAC 50/60 Hz

SWT-MIP-BLC-1 2210013 Swift™ MiniPro® Block 1 (24 x 0.2 ml)

SWT-MIP-BLC-2 2210014 Swift™ MiniPro® Block 2 (18 x 0.5 ml)

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Swift™ MiniPro® Conventional PCR Thermal Cycler

Swift™
Spectrum 48 Real-Time
PCR Detection System

Swift SpectrumTM 48 Real Time Thermal Cycler

Esco’s Swift™ Spectrum 48 real time PCR detection system is specially designed to simplify and optimize your PCR work. The
advanced Ferrotec peltier, proprietary linear block, unique bottom detection design and coaxial fiber optic technology provide
excellent temperature performance and reliable fluorescence detection results. The Spectrum PC software offers maximum
flexibility for data processing of a variety of scientific research and clinical applications, such as gene expression analysis, SNP
genotyping, pathogen detection and others.

Additional features
• With up to 4 groups of filters, the instrument covers most wavelengths of commonly used dyes.

• Unique bottom detection design with coaxial fiber optics avoids crosstalk among wells, increases the signal-to-noise ratio
and ensures reliable results.

• Precisely tuned Ferrotec peltier module + proprietary temperature control algorithms = excellent temperature accuracy +
industry-leading reliability. Temperature accuracy: <±0.1 ºC

• The unique sandwich-design block, with peltier elements closely fit both sides of the linear sample block, delivers rapid,
controlled temperature changes and reduces vertical temperature gradients. Maximum reproducibility and highest quality
results are ensured.

• Temperature uniformity: <±0.3 ºC

• Volume sensor software automatically adjusts ramp rates to accommodate differences in sample volumes.

• An automatic hot lid with adjustable temperature effectively prevents reagent evaporation.

• Wide block temperature range from 4 ºC to 99.9 ºC, with infinity hold function that allows PCR products to be stored at
4 ºC overnight.

• Open platform chemistry and consumables ensure compatibility with commonly used protocols.

• The block can be divided into a maximum 4 segments, allowing the analysis of up to 4 different sample groups.

• The overheating protection function sounds an audible warning at excessive ambient temperature and automatically shuts
down the instrument when ambient temperature reaches 40 ºC.

• Global wide range power supply with PFC function improves thermal efficiency and reduces power consumption by 30%.
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Swift™ Spectrum 48 Real-Time PCR Detection System

General Specifications, Swift™ Spectrum 48 Real Time PCR Detection System

Model Code SWT-SPT-RT-48

Sample Capacity 48 × 0.2 ml PCR tubes ( Bottom Transparent), 6 x 8 strips,

Optical module

Excitation LEDs

Detection 1 photo-multiplier tube for 4 channels

Excitation Wavelength 450-590 nm

Emission Wavelength 500-630 nm

F1: FAM, SYBER Green I

F2: VIC, HEX
Channel And Fluorescence
F3: JOE, Cy3, TAMRA
F4: ROX, TEXAS-RED

Thermal Cycler

Maximum Block Heating Rate* 4.0°C / sec

Maximum Block Cooling Rate* 4.0°C / sec

Temperature Accuracy ±0.1°C

Temperature Uniformity ±0.3°C

Temperature Range 4-99.9°C

Hot Lid Temperature Range 80-110°C (Adjustable, Default 105°C, Automatic Hot-lid)

Spectrum PC software

Operation System Windows XP / Windows Vista / Windows 7 / Windows 8

PC Configuration Memory: 512M, Hard Disk: 10GB, CPU: Pentium 4, Minimum Virtual Memory: 1000MB

Data Analysis Methods Absolute Quantification, Standard Curve, Relative Quantification, Melting Curve, SNP Genotyping

Complete System

PCR Sample Volume 5-100 μL (10-40 μL recommended)

Interface RS232C for PC control

Dimensions (W × D × H) 450 x 520 x 320 mm (17.7’’ x 20.5’’ x 12.6’’)

Power Supply, Consumption 100-240 VAC, 50/60 Hz, 650 W

Electrical Approvals CE

Warranty 2 years

Net Weight 25 Kg (55.1 lbs)

Shipping Weight 26 Kg (57.3 lbs)

Shipping Dimensions 590 x 680 x 470 mm

(W x D x H) (23.2’’ x 26.8’’ x 18.5’’)

Ordering information
Ordering Information, Swift™ Spectrum 48 Real Time PCR Detection System Specifications

Model Code Item Code Description

SWT-SPT-RT-48 2210031 Swift™ Spectrum RT Cycler 48 110-220 VAC

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Swift™ Spectrum 48 Real-Time PCR Detection System

Sample Tube

Sample Module Module

Filter
Heat Sink
PMT

Excitation Light Module

Coaxial Optical Fiber Hot Lid

Temp. gradient control

up to 24˚ gradient

Stepper Motor
Stepper Motor
Coaxial Optical Fiber

Outstanding Design, thus Excellent Performance

• The LED in AccuFluore™ Detection System provides a wide range of stable excitation, allowing more dye flexibility. It has a
longer lifespan in contrast to halogen lamps without the need for calibration.

• Photomultiplier tube (PMT) is used as detection sensors, covering up to 4 channels. The PMT, manufactured by the world’s
top PMT manufacturer, is almost noise free, with superior sensitivity and reproducibility. Its high signal-to-noise ratio allows
detection of even a single molecule.

• The coaxial fiber optic system makes sure the same amount of excitation light is received by each well and uniform signal
measurement is obtained from each well, so no additional signal correction and calibrations are needed. Besides, unlike
normal CCD which detects the signals from all wells at a time, the coaxial fiber optic system allows signals detection from the
bottom of the tubes one by one, avoiding crosstalk among wells. Over a linear dynamic range, the system can detect over
10 levels of magnitude.

Proprietary Sandwich Design Block

Unlike conventional 12 x 8, 96 well block designs, Spectrum 48 real-time PCR uses a unique 48 well linear block to deliver
rapid, controlled temperature changes.

The Peltier elements are manufactured by the world’s top Peltier manufacturer. The heating/cooling elements are positioned
along the two sides of the block, guaranteeing rapid, equal heating and cooling rates of 4°C / sec, as well as exceptional
temperature accuracy and uniformity. The block design eliminates temperature overshoots and undershoots, increasing the
efficiency of the reaction, resulting in high template yields and low copy number detection. Fast cycling is not dependent on
the use of specific reagents and reduces the cycle run producing a result in around 1 hour.

The block allows signal detection from the bottom of the tube, reducing signal scatter through the tube cap, or from fogging
of the cap from sample evaporation. Sensitivity is also enhanced because of the shorter light path between the reagent and
the detector. It is also possible to divide the block into 4 segments, allowing the simultaneous analysis of up to 4 different
sample groups.

Sandwich Design Linear Block Sample Grouping

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Swift™ Spectrum 48 Real-Time PCR Detection System

Powerful Software, Simple to Operate

Spectrum PC software’s simple intuitive navigation makes it easy to set up sample data, PCR protocols and get excellent real
time PCR results. Real-time amplification can be monitored and data file will be automatically saved when a run is finished.
Data files can also be exported to Microsoft Excel for further analysis. The software has built-in data analysis methods,
including Absolute Quantification, Standard Curve, Relative Quantification, Melting Curve and SNP Genotyping.

Protocol Setup Quantification and Standard Curve

Melting Curve Analysis Multiplex Analysis

SNP Genotyping Analysis Relative Quantification

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Swift™ Spectrum 96 Real-Time PCR Detection System

Swift™
Spectrum 96 Real-Time
PCR Detection System

Swift™ Series Thermal Cyclers,

Spectrum 96 Real-Time PCR Detection System

Esco introduces the new Swift™ Spectrum 96 real time PCR detection system with up to 8 channels to meet all your PCR
needs. The advanced top quality peltier, proprietary block dissipation technology, unique bottom detection design and coaxial
fiber optic technology provide excellent temperature performance and reliable fluorescence detection results. The Spectrum
PC software offers maximum flexibility for data processing of a variety of scientific research and clinical applications, such as
gene expression analysis, SNP genotyping, pathogen detection and others.

additional Features
• With up to 4/8 groups of filters, the instrument covers all wavelengths of commonly used dyes.
• Unique bottom detection design with coaxial fiber optics avoids crosstalk among wells, increases the signal-to-noise ratio
and ensures reliable results.
• Precisely tuned peltier module + proprietary temperature control algorithms = excellent temperature accuracy + industry-
leading reliability. Temperature accuracy: <±0.1 ºC
• The unique TAS temperature control technology avoids the edge effect of block heat conduction and therefore guarantees
extremely uniform temperature between central and edge wells. Super reproducibility and highest quality results are
ensured. Temperature uniformity: <±0.3°C
• Proprietary block heat dissipation technology brings on high heating and cooling rate of up to 4.0°C / sec, allowing
significantly shorter cycle times.
• Automatic temperature control mode (Tube/ Block) switches based on sample volume.
• An automatic hot lid with adjustable temperature effectively prevents reagent evaporation.
• Optimal results obtained with sample volumes as low as 5 μL, minimizes the use of sample and reagents and saves cost for
your laboratory.
• Wide block temperature range from 4 ºC to 105 ºC, with infinity hold function allowing PCR products to be stored at 4 ºC
overnight.
• Open platform chemistry and consumables ensure compatibility with commonly used protocols.
• Entire micro-plate scan and designated line scan are available for choice. A 96-wells dual-channel scan only takes 5.5s.
• RS232 C, USB or bluetooth provide configuration flexibility and enable PC free operation.
• Global wide range power supply with PFC function.
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Swift™ Spectrum 96 Real-Time PCR Detection System

General Specifications, Swift™ Spectrum 96 Real Time PCR Detection System

Model SWT-SPT-RT-96-4 SWT-SPT-RT-96-8

Sample Capacity 96 × 0.2 ml PCR tubes (Bottom Transparent), 12 x 8 strips, 96-Well PCR plate (full-skirted)

Optical module

Excitation LEDs

Detection 1 photo-multiplier tube for 4 channels 2 photo-multiplier tubes for up to 8 channels

Excitation Wavelength 300-800 nm

Emission Wavelength 500-800 nm

F1: FAM, SYBER Green I; F2: VIC, HEX, TET, JOE

F1: FAM, SYBER Green I; F2: VIC, HEX, TET, JOE; F3: CY3, NED,TAMRA; F4: ROX TEXAS-RED
Channel And Fluorescence F3: CY3, NED,TAMRA; F4: ROX TEXAS-RED F5: CY5; F6: LightCycler Red
F7 and F8 for customized purpose

Thermal Cycler

Maximum Block Heating/

4.0°C /sec
Cooling Rate*
Temperature Accuracy ±0.1°C

Temperature Uniformity ±0.3°C

Temperature Range 4-105°C

Hot Lid Temperature Range 30-110°C (Adjustable, Default 105°C, Automatic Hot-Lid)

Temperature Control Mode Tube or Block

Spectrum PC software

Operation System Windows 2000 / Windows XP / Windows Vista / Windows 7 / Windows 8

PC Configuration Memory: 512M, Hard Disk: 10GB, CPU: Pentium 4, Minimum Virtual Memory: 1000MB

Multiplex Analysis Up to 4 targets per well Up to 8 targets per well

Scan Mode Entire plate or designated line

Scan Time 5.5 s ( F1/F2 full 96-well plate scan)

Data Analysis Methods Absolute Quantification, Standard Curve, Relative Quantification, Melting Curve, SNP Genotyping

Complete System

PCR Sample Volume 5-100 μL

Interface 1 x RS232C, 1 x USB, 1 x Bluetooth for PC control

Dimensions (W × D × H) 395 x 430 x 352 mm (15.5’’ x 16.9’’ x 13.9’’)

Power Supply, Consumption 100-240 VAC, 50/60 Hz, 600 W

Electrical Approvals CE

Warranty 2 years

Net Weight 28 Kg (61.7 lbs)

Shipping Weight 29 Kg (63.9 lbs)

Shipping Dimensions 590 x 680 x 470 mm

(W x D x H) (23.2’’ x 26.8’’ x 18.5’’)

*Measurements on the block.

Ordering information
Ordering Information, Swift™ Spectrum 96 Real Time PCR Detection System

Model Code Item Code Description

SWT-SPT-RT-96-8 2210001 Swift™ Spectrum RT Cycler 96 110-220 VAC 8 channels

SWT-SPT-RT-96-4 2210002 Swift™ Spectrum RT Cycler 96 110-220 VAC 4 channels

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Swift™ Spectrum 96 Real-Time PCR Detection System

High Precision Wide Detection Range

SNPs Detection Analysis of SNPs

Outstanding Design, thus Excellent Performance

Specially Designed Peltier Elements for Bottom Detection System

Spectrum is the only real-time PCR that use both Peltier technology and bottom detection
at the same time. The 96-well block use the unique, proprietary multi-holes peltier module,
designed to deliver rapid, controlled temperature changes and allow signal detection from
the bottom of the tube, reducing signal scatter through the tube cap, or from fogging of
the cap from sample evaporation. Sensitivity is also enhanced because of the shorter
Reliable Performance Peltier light path between the reagent and the detector. It is also possible to divide the block
into 4 segments, allowing the simultaneous analysis of up to 4 different sample groups.

Proprietary FastCoolTM Block Heat Dissipation Technology

Unlike conventional peltier based cyclers which use heat sinks to remove the heat, Spectrum
real-time PCR uses heat pipes in addition to active fan-based heat sinks to provide fast, even
heat dissipation and minimize the footprint of the cycler to save limited bench top space in
the lab. Heat pipes have a much higher effective thermal conductivity than solid materials,
4 Segments Heating
Cooling Block thus can quickly transfer heat from block to heat sink and dissipate the heat, providing high
block heating and cooling rates of 4°C/sec. Fast cycling is not dependent on the use of specific
reagents and reduces the cycle run producing a result in around 1 hr.

AccuFluoreTM Detection System

• The LED in AccuFluore™ Detection System provides

a wide range of stable excitation, allowing more
dye flexibility. It has a longer lifespan in contrast to FastCool™ Block Heat Dissipation Technology
halogen lamps and no calibration is required.
• One/Two Photomultiplier tubes (PMT) are used Sample Tube
as detection sensors, covering up to 4/8 channels.
Peltier
The PMT, manufactured by the world’s top PMT Coaxial Optical Fiber
manufacturer, is almost noise free, with superior
sensitivity and reproducibility. Its high signal to noise
ratio allows even single molecule detection. Over
a linear dynamic range the system detects over 10 Stepper Motor (X)
levels of magnitude.
Filter
• The coaxial fiber optic system makes sure the same
amount of excitation light is received by each well
and uniform signal measurement is obtained from Stepper Motor (Y)

each well, so no additional signal correction and

calibrations are needed. Besides, unlike normal CCD, • With the AccuFluoreTM detection system, the Spectrum 96 is a multi-
which detects the signals from all wells at a time, channel instrument with up to 4/8 usable channels. The excitation
the coaxial fiber optic system allows signals detection wavelength range is from 300 nm to 800 nm and the emission wavelengths
from the bottom of the tubes one by one, avoiding are between 500 nm and 800 nm. Up to 6 channels are fixed for the most
crosstalk among wells. current commercially available dyes, and optional 7th and 8th channels are
available if required.
 21
Swift™ Spectrum 96 Real-Time PCR Detection System

Powerful Software, Simple to Operate

Spectrum PC software’s simple intuitive navigation makes it easy to set up sample data, PCR protocols and get excellent real time PCR
results. Real-time amplification can be monitored and data file will be automatically saved when a run is finished. Data files can also be
exported to Excel for further analysis. The software has built-in data analysis methods, including Absolute Quantification, Standard Curve,
Relative Quantification, Melting Curve and SNP Genotyping.

Well and Dye Setting Quantitative Analysis

Standard Curve Melting Curve

Relative Quantification Analysis SNP Interface

 22
Provocell™ Shaking Micro Incubator

Provocell™
Shaking Micro Incubator

Provocell™ Shaking Micro Incubator

The Esco Provocell™ Shaking Micro Incubator is designed for a wide variety of mixing applications for accurate incubation of
reactions and denaturation of nucleic acids and proteins. Provocell™ combines an advanced microprocessor-based controller
with Peltier heating and cooling to deliver outstanding reliability, safety and overall performance.
• Provocell™ can be used on the benchtop or in a biological safety or laminar flow cabinet without the contamination risk
associated with conventional water- or liquid-cooling baths.
• Peltier technology permits rapid switching between heating and cooling with accurate temperature control and block uniformity.
• Special, stress-release ceramics prevent block damage resulting from rapid temperature changes, prolonging block lifespan.
• The Provocell™ system is environmentally friendly, maintenance free and uses no refrigerants or coolants.

General Features User Friendly Operation

• Smooth orbital rotation The large Vacuum Fluorescent Display (VFD) gives the user
a clear view of the current temperature, speed and time.
• Digitally controlled Peltier heating and cooling
• Operational parameters are color coded for easy visual
• Fully programmable with speed setting up to 1500 rpm
differentiation of the parameters.
• User-friendly interface
• A state-of-the-art microprocessor with pre-programmed
• Large, easy to read display interface is easy to use and allows the operator to modify
• Easy to clean interchangeable metal blocks temperature, time and speed during operation.
• Small footprint
• Compatible for use inside biological safety cabinet High Performance Peltier Modules
• Manufactured with top quality, laboratory-grade Peltier modules are designed for rapid temperature heating
components or cooling response and overall temperature accuracy.
• Excellent temperature uniformity.
Uniform Mixing • Temperature control accuracy, ΔT is less than 0.1°C.
The Provocell™ Shaking Micro Incubator delivers stable • Ceramic semiconductors eliminate moving parts and
orbital rotation creating a steady vortexing that is required noise, reducing vibration, minimizing maintenance.
to ensure even and accurate mixing conditions. • Lightweight modules occupy a small footprint.
• Rotation speed can be adjusted up to 1500 rpm within a • An aluminum covering and powerful fan dissipates heat
3 mm diameter (0.11”) rotation axis. efficiently and quickly.
• The sample block is mounted to the main body using 4 • The unique module design reduces heat loss.
bolts to enhance stability.
• The ergonomic design is easy to use.
• The long motor life minimizes maintenance costs.
 23
Provocell™ Shaking Micro Incubator

General Specifications, ProvocellTM Shaking Micro Incubator

Order Number Capacity/Size

BLC-1 1.5 ml x 40 (Standard)

BLC-2 0.2 ml x 96

BLC-3 0.5 ml x 54
Available Blocks*
BLC-4 Ø15 mm x 24

BLC-5 96 wells ELISA board

BLC-6 0.5 ml x 26 + 1.5 ml x 24

BLC-7 2 ml x 40

Temperature Setting Range 0-105°C

Temperature Control Range Ambient -14°C to 100°C

Block Temperature Uniformiity ±0.5°C

Temperature Accuracy ±0.5°C

Temperature Uniformity ±0.5°C

Heating Rate 6°C / min (from 20°C to 100°C)

Heating Time, Nominal ≤12 mins from 20°C to 100°C

Cooling Time, Nominal ≤8 mins for 10°C decrease from ambient temperature ≤15 mins from 100°C to 10°C above ambient temperature

Timer Range 1 min to 99 h 59 min

Speed 300-1500 rpm

Amplitude 3 mm (0.11")

Model Voltage

Power supply, Consumption ESC-PVC-2 110 VAC±11V, 50/60 Hz, 150 W

ESC-PVC-1 220 VAC±22V, 50/60 Hz, 150 W

295 x 265 x 170 mm

Dimension (W x D x H)
(11.6" x 10.4" x 6.7")

Warranty 3 years for mainbody, 2 years for blocks

Net Weight 9.5 Kg (20.9 lbs)

Shipping Weight 10.5 Kg (23.1 lbs)

Shipping Dimensions 320 x 420 x 330 mm

(W x D x H) (12.6’’ x 16.5’’ x 13.0’’)

*Note to customer: Specify block when ordering.

Ordering information
Order Information, ProvocellTM Shaking Micro Incubator

Model Code Item Code Description

ESC/PV-PVC-2 2210029 Provocell™ Micro Incubator 110 VAC

ESC/PV-PVC-1 2210030 Provocell™ Micro Incubator 220 VAC

ESC/PV-BLC-1 2210022 Provocell™ Incubator Block 1 (1.5 ml x 40)

ESC/PV-BLC-2 2210023 Provocell™ Incubator Block 2 (0.2 ml x 96)

ESC/PV-BLC-3 2210024 Provocell™ Incubator Block 3 (0.5 ml x 54)

ESC/PV-BLC-4 2210025 Provocell™ Incubator Block 4 (15 mm x 24)

ESC/PV-BLC-5 2210027 Provocell™ Micro Incubator Block 5 96 Well ELISA Board

ESC/PV-BLC-6 2210028 Provocell™ Micro Incubator Block 6 26 x 0.5 ml + 24 x 1.5 ml

ESC/PV-BLC-7 2210026 Provocell™ Incubator Block 7 2 ml x 40

 ESCO Global Network

ART Equipment
Biological Safety Cabinets
CO2 Incubators
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Laboratory Ovens and Incubators
Laminar Flow Clean Benches
PCR Cabinets
PCR Thermal Cyclers
Powder Weighing Balance Enclosures
Ultra-low Freezers

The Esco Group of Companies is a global life sciences tools provider with sales in over 100 countries. The group is active in
lab equipment, pharma equipment and medical devices. Manufacturing facilities are located in Asia and Europe. R&D is
conducted worldwide spanning the US, Europe and Asia. Sales, service and marketing subsidiaries are located in 12 major
markets including the US, UK, Singapore, Japan, China and India. Regional distribution centers are located in the US, UK,
and Singapore.

Life Science • Chemical Research • Assisted Reproductive

Technology (ART) • Pharmaceutical Equipment • General Equipment
products and specifications without notice. All trademarks and logotypes in this material are the property of Esco and the respective companies.
Esco can accept no responsibility for possible errors in catalogues, brochures and other printed materials. Esco reserves the right to alter its
9010294_PCR_Thermal Cyclers_combined_Catalog_vC_051916

Esco Micro Pte. Ltd. • 21 Changi South Street 1 • Singapore 486 777
Tel +65 6542 0833 • Fax +65 6542 6920 • [email protected]
www.escoglobal.com

Esco Technologies, Inc. • 903 Sheehy Drive, Suite F, Horsham, PA 19044, USA
Toll-Free USA and Canada 1-877-479-3726 • Tel 215-441-9661 • Fax 484-698-7757
[email protected] • www.escolifesciences.us
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