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    Blood Glucose Test: Written By:marwa Azad Ahmed Supervised By:dr - Lubna.Sameer

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    فنر محمد
    A blood glucose test measures the levels of glucose in the blood. Glucose is the body's main source of energy and insulin helps regulate its levels. [1] The test is used to diagnose and monitor diabetes, which occurs when glucose levels are too high (hyperglycemia) or too low (hypoglycemia). [2] The procedure involves drawing blood, separating its components, mixing the serum with reagents, and measuring absorbance to calculate glucose concentration. [3] Abnormal results may indicate prediabetes or diabetes and require further testing for diagnosis.

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    Blood Glucose Test: Written By:marwa Azad Ahmed Supervised By:dr - Lubna.Sameer

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    فنر محمد
    68 views8 pages
    A blood glucose test measures the levels of glucose in the blood. Glucose is the body's main source of energy and insulin helps regulate its levels. [1] The test is used to diagnose and monitor diabetes, which occurs when glucose levels are too high (hyperglycemia) or too low (hypoglycemia). [2] The procedure involves drawing blood, separating its components, mixing the serum with reagents, and measuring absorbance to calculate glucose concentration. [3] Abnormal results may indicate prediabetes or diabetes and require further testing for diagnosis.

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    Glucose test

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    Blood-Glucose-Test

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    A blood glucose test measures the levels of glucose in the blood. Glucose is the body's main source of energy and insulin helps regulate its levels. [1] The test is used to diagnose and monitor diabetes, which occurs when glucose levels are too high (hyperglycemia) or too low (hypoglycemia). [2] The procedure involves drawing blood, separating its components, mixing the serum with reagents, and measuring absorbance to calculate glucose concentration. [3] Abnormal results may indicate prediabetes or diabetes and require further testing for diagnosis.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    68 views8 pages

    Blood Glucose Test: Written By:marwa Azad Ahmed Supervised By:dr - Lubna.Sameer

    Uploaded by

    فنر محمد
    A blood glucose test measures the levels of glucose in the blood. Glucose is the body's main source of energy and insulin helps regulate its levels. [1] The test is used to diagnose and monitor diabetes, which occurs when glucose levels are too high (hyperglycemia) or too low (hypoglycemia). [2] The procedure involves drawing blood, separating its components, mixing the serum with reagents, and measuring absorbance to calculate glucose concentration. [3] Abnormal results may indicate prediabetes or diabetes and require further testing for diagnosis.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
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    ‫قسم تقنيات التمريض‬/‫معهد تقني طبي‬/ ‫الجامعة التقنية الشمالية‬

    Blood Glucose Test


    Written by:Marwa Azad Ahmed

    Supervised by:Dr.Lubna.Sameer
    Table of Contents
    1.Introduction

    2.What is it used for


    3. Symptoms of high blood glucose 

    4. Symptoms of low blood glucose levels

    5.Materials and Methods

    6. Specimen collection
    7. Procedure
    8. Calculation
    9. Result
    10. Discussion
    11.Reference
    Introduction
    Blood Glucose Test
    A blood glucose test measures the glucose levels in your blood. Glucose is a type
    of sugar. It is your body's main source of energy. A hormone called insulin helps
    move glucose from your bloodstream into your cells. Too much or too little
    glucose in the blood can be a sign of a serious medical condition. High blood
    glucose levels (hyperglycemia) may be a sign of diabetes, a disorder that can
    cause heart disease, blindness, kidney failure and other complications. Low blood
    glucose levels (hypoglycemia) can also lead to major health problems, including
    brain damage, if not treated.

    Laboratory tests are often used to diagnose illnesses and such methods
    include

     fasting blood sugar (FBS), fasting plasma glucose (FPG): 10–16 hours after
    eating
     glucose tolerance test: continuous testing
     postprandial glucose test (PC): 2 hours after eating
     random glucose test

    What is it used for?


    A blood glucose test is used to find out if your blood sugar levels are in the
    healthy range. It is often used to help diagnose and monitor diabetes.
    Symptoms of high blood glucose levels include:

     Increased thirst
     More frequent urination
     Blurred vision
     Fatigue
     Wounds that are slow to heal

    Symptoms of low blood glucose levels include:

     Anxiety
     Sweating
     Trembling
     Hunger
     Confusion

    Will I need to do anything to prepare for the test?


    You will probably need to fast (not eat or drink) for eight hours before the test. If
    you are pregnant and are being checked for gestational diabetes:
     You will drink a sugary liquid one hour before your blood is drawn.
     You won't need to fast for this test.
     If your results show higher than normal blood glucose levels, you may need another
    test, which requires fasting.
    Materials and Methods
    1.Alcohol
    2.Cotton
    3.Tourniquet
    4.Syringe
    5.Test Tube
    6.Pipette
    7.Centrifuge
    8.Incubator
    9.Spectrophotometer

    Specimen collection:
    Serum or plasma

    Procedure
    First we draw blood and put blood in a test tube, then put the tube in the
    centrifuge for 5 minutes to separate the blood components. Then we
    prepare 3 tubes and name the three tubes. The first tube is called Blank, in
    which we put Reagent and Demineralised Water, the second tube called
    Standard, in which we put Regent and Standard. The third tube is called the
    Assay, and we put in it a reagent and withdraw the serum from the tube after
    separating it from its components.Mix.Let stand for 10 minutes at 37 or 20 minutes at
    Room temperature.Read absorbance at 500 nm (460-560) against reagent blank.

    Calculation
    Abs(Assay):100

    Abs(Standard):40

    Wave Length:500 nm

    Calculate the result as follows:

    Result=Abs(Assay)/Abs(Standard) × Standard Concentration

    =100/40 × 100
    =250 mg/dl

    Result
    results show higher than normal blood glucose levels.
    Discussion

    Abnormal results

    If you had a fasting blood glucose test, the following results are
    abnormal and indicate you may have either prediabetes or diabetes:

     A blood glucose level of 100–125 mg/dL indicates that you have


    prediabetes.

     A blood glucose level of 126 mg/dL and higher indicates that you
    have diabetes.

    If you had a random blood glucose test, the following results are
    abnormal and indicate you may have either prediabetes or diabetes:

     A blood glucose level of 140–199 mg/dL indicates that you may


    have prediabetes.

     A blood glucose level of 200 mg/dL and higher indicates that you
    likely have diabetes.

    If your random blood glucose test results are abnormal, your doctor will
    probably order a fasting blood glucose test to confirm the diagnosis or
    another test such as an Hgba1c.

    What do the results mean?


    If your results show higher than normal glucose levels, it may mean you have or
    are at risk for getting diabetes. High glucose levels may also be a sign of:

     Kidney disease
     Hyperthyroidism
     Pancreatitis
     Pancreatic cancer
    Reference
    1.
    www.medline.com
    2.  Khatib, Oussama MN (2006). Guidelines for the
    prevention, management and care of diabetes mellitus.
    World Health Organization, Regional Office for the
    Eastern Mediterranean
    3.
    www.healthline.com
    4.
    www.biolabo.fr

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