Scribd Logo
Upload

Welcome to Scribd!

  • 21 views

    Total - Protein Meron Kit

    Uploaded by

    Ranjit Pathak
    This document provides information on a total protein reagent kit used to determine total protein levels in serum or plasma using the biuret method. The kit contains a biuret reagent and protein standard that are stable until the expiry date when stored at 2-8°C. Blood should be collected and separated within 60 minutes, with proteins stable in serum/plasma for 7 days at 2-8°C or 1 month frozen. The biuret method involves a colorimetric reaction between proteins and copper ions under alkaline conditions to form a blue complex, with intensity proportional to protein concentration. The assay is run on automated analyzers or manually, with a normal protein range of 6.3-8.4 gm/

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Total - Protein Meron Kit

    Uploaded by

    Ranjit Pathak
    21 views2 pages
    This document provides information on a total protein reagent kit used to determine total protein levels in serum or plasma using the biuret method. The kit contains a biuret reagent and protein standard that are stable until the expiry date when stored at 2-8°C. Blood should be collected and separated within 60 minutes, with proteins stable in serum/plasma for 7 days at 2-8°C or 1 month frozen. The biuret method involves a colorimetric reaction between proteins and copper ions under alkaline conditions to form a blue complex, with intensity proportional to protein concentration. The assay is run on automated analyzers or manually, with a normal protein range of 6.3-8.4 gm/

    Original Description:

    Total_Protein Meron kit

    Original Title

    Total_Protein Meron kit

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides information on a total protein reagent kit used to determine total protein levels in serum or plasma using the biuret method. The kit contains a biuret reagent and protein standard that are stable until the expiry date when stored at 2-8°C. Blood should be collected and separated within 60 minutes, with proteins stable in serum/plasma for 7 days at 2-8°C or 1 month frozen. The biuret method involves a colorimetric reaction between proteins and copper ions under alkaline conditions to form a blue complex, with intensity proportional to protein concentration. The assay is run on automated analyzers or manually, with a normal protein range of 6.3-8.4 gm/

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    21 views2 pages

    Total - Protein Meron Kit

    Uploaded by

    Ranjit Pathak
    This document provides information on a total protein reagent kit used to determine total protein levels in serum or plasma using the biuret method. The kit contains a biuret reagent and protein standard that are stable until the expiry date when stored at 2-8°C. Blood should be collected and separated within 60 minutes, with proteins stable in serum/plasma for 7 days at 2-8°C or 1 month frozen. The biuret method involves a colorimetric reaction between proteins and copper ions under alkaline conditions to form a blue complex, with intensity proportional to protein concentration. The assay is run on automated analyzers or manually, with a normal protein range of 6.3-8.4 gm/

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    TOTAL PROTEIN

    (Biuret Method)
    Intended Use
    Total protein is a reagent kit used for the determination of total protein in serum or plasma based on Biuret method. The
    reagents are for in vitro diagnostic use.
    Principle
    Proteins react with cupric ions under alkaline pH to produce a coloured complex. The coloured complex absorbs light
    at 546 nm (530 - 570 nm). The intensity of the colour is directly proportional to the protein concentration in specimen.
    Cu2+, Alkaline pH
    Proteins Blue color complex
    Reagent Provided
    1. Biuret Reagent - Ready to use.
    2. Standard - Total Protein (6 gm/dl).
    Reagent storage and stability
    Biuret reagent and standard are stable till the expiry date indicated on the bottle label when stored at 2° - 8°C.
    Specimen collection and preservation
    Blood should be collected in a clean dry container. Plastic or siliconized container should be avoided as it may prolong
    clotting time. Serum or plasma should be separated from the cells within 60 minutes. For plasma separation following
    anticoagulants may be used.
    • EDTA 2 mg/ml of blood
    • CITRATE 6 mg/ml of blood
    • HEPARIN 200 IU/ml of blood
    • OXALATE 3 mg/ml of blood
    • SODIUM FLUORIDE 10 mg/ml of blood
    Proteins are stable in the serum and plasma for 7 days when stored at 2° - 8°C and for a month when stored at -20°C
    or frozen.
    Assay guidelines for Analyzers
    Reaction type End point with standard
    Reaction slope Increasing
    Incubation time 5 minutes at 37oC
    Wavelength 546 nm (530 - 570 nm)
    Blank Reagent Blank
    Blank absorbance limit < 0.300 Abs. against distilled water blank.
    Sample volume 20 µl (0.02 ml)
    Reagent volume 1000 µl (1.0 ml)
    Standard concentration 6 gm/dl
    Factor calculation 6 gm/dl ÷ Abs. of Std.
    Low Normal 6.3 gm/dl
    High Normal 8.4 gm/dl
    Linearity Up to 18 gm/dl
    Assay guidelines for Manual procedure
    Bring the reagent and standard to room temperature before performing the assay.
    Reagents Blank Standard Sample
    Biuret Reagent 1000 µl (1.0ml) 1000 µl (1.0ml) 1000 µl (1.0ml)
    Standard - 20 µl (0.02 ml) -
    Sample - - 20 µl (0.02 ml)
    1. Mix thoroughly and incubate at 37oC for 5 minutes.
    2. Read the absorbance against reagent blank at 546 nm (530 - 570 nm).
    3. The final colour is stable for 1 hour, if not exposed to light.

    V: TPL1- IV Page 1
    Calculation
    Con. in sample (gm/dl) = Sample OD x Con. of Std.
    Std OD
    Normal Range
    Guidance value : 6.3 - 8.4 gm/dl
    Note: Expected range varies from population to population and each laboratory should establish its own normal range.
    Limitation
    • The working reagent is considered unsatisfactory and should not be used if it develops insoluble precipitate.
    • The reagent is linear up to 18 gm / dl. For higher value, dilute sample with normal saline and perform the assay.
    Multiply the final result by dilution factor to get the real value.
    • For haemolysed or icteric samples (Bilirubin > 5 mg %) a saline blank should be run along with the assay.
    Read the absorbance of saline blank against distilled water and subtract it from sample absorbance.
    Quality Control
    To ensure adequate quality control measures, it is recommended that each batch should include a normal and an
    abnormal commercial reference control serum. It should be realized that the use of quality control material checks both
    instrument and reagent functions together. Factors which might affect the performance of this test include proper
    instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
    References
    1. Stirkland R.D., et al. Anal, Chem. 33, (1961).
    2. Henry, R.J., et al, “Clinical Chemistry- principles and technics” Harper & Row, II ed. (1974).

    Marketed By:
    Meron Scientific Pvt. Ltd.
    112, 1st Floor, A Wing
    R.G.Metro Arcade
    Sector-11, Rohini
    New Delhi-110085
    Web: www.meronscientific.com
    E : [email protected]
    Tel. : +91 - 8882715885/ 011-42208042
    Toll Free : 1800121000050

    WHO GMP Compliance ISO 9001: 2015 & ISO 13485: 2016 Certified Company

    V: TPL1- IV Page 2

    You might also like