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    GFP Model

    Uploaded by

    Itzel López González
    The document provides instructions for building a paper model of green fluorescent protein (GFP). GFP is a protein that exhibits fluorescence and is widely used in biotechnology. The model represents the primary and secondary structure of GFP, including 11 beta strands and 1 helical region that form a beta barrel shape. When completed, the model allows users to identify the polymer chain from amino to carboxy terminus, understand the relationship between strand labels and numbers, and recognize examples of parallel and antiparallel strands in the GFP barrel.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    GFP Model

    Uploaded by

    Itzel López González
    348 views2 pages
    The document provides instructions for building a paper model of green fluorescent protein (GFP). GFP is a protein that exhibits fluorescence and is widely used in biotechnology. The model represents the primary and secondary structure of GFP, including 11 beta strands and 1 helical region that form a beta barrel shape. When completed, the model allows users to identify the polymer chain from amino to carboxy terminus, understand the relationship between strand labels and numbers, and recognize examples of parallel and antiparallel strands in the GFP barrel.

    Original Description:

    Modelo 3D en papel de la GFP

    Original Title

    gfp-model

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    The document provides instructions for building a paper model of green fluorescent protein (GFP). GFP is a protein that exhibits fluorescence and is widely used in biotechnology. The model represents the primary and secondary structure of GFP, including 11 beta strands and 1 helical region that form a beta barrel shape. When completed, the model allows users to identify the polymer chain from amino to carboxy terminus, understand the relationship between strand labels and numbers, and recognize examples of parallel and antiparallel strands in the GFP barrel.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    348 views2 pages

    GFP Model

    Uploaded by

    Itzel López González
    The document provides instructions for building a paper model of green fluorescent protein (GFP). GFP is a protein that exhibits fluorescence and is widely used in biotechnology. The model represents the primary and secondary structure of GFP, including 11 beta strands and 1 helical region that form a beta barrel shape. When completed, the model allows users to identify the polymer chain from amino to carboxy terminus, understand the relationship between strand labels and numbers, and recognize examples of parallel and antiparallel strands in the GFP barrel.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    Build a Paper Model of Green Fluorescent Protein (GFP)

    pdb101.rcsb.org GFP is a protein that can exhibit light. It is widely used in biotechnology.

    Step1 Step2 The Primary Structure


    Cut out the 12 strips of Tape together the strips The long strip of paper from
    paper (outlined in dotted so that the lowercase letter amino (NH3+) to carboxy
    line) representing the 11 on the white background (COO-) represents the
    beta strands and 1 helical (e.g., a) is taped on top of primary structure of GFP.
    region of the GFP. the same letter on the Regions of secondary structure
    shaded background. Repeat (beta strands and alpha
    this for letters b through k helices) are marked.
    to create a long strip.

    Step 3 Step 4
    K
    alternate the arrows

    J
    Lay the strand labeled A on Next tape strand C next to B, D next to C and

    I
    *
    the table so that the arrow is so on till strand K, in each case aligning the grey
    H
    pointing upwards, place areas between the strands and making sure the
    G
    strand B to the right of A and align
    F
    arrows in these strands point up and down
    &
    E
    orient it so that the arrow alternately as shown in the diagram on the right.
    tape
    D
    points to the bottom. Align align
    At the end of this step the beta sheet should
    C
    the grey areas between the

    B
    have strands labeled A-K reading from
    strands (representing the
    A
    0.8035 in

    left to right.
    hydrogen bonds between
    them), and tape top to bottom At this point make sure the chromophore move the chromophore strip
    on the aligned grey area. strip is behind the printed side from the front (shown)
    of the beta sheet. to the back of the printed side

    The GFP model was created using


    Step 5 Exploring the Model data from the PDB archive
    Close the GFP beta barrel 1. Can you trace the polymer chain from the PDB ID: 1EMA
    by aligning the grey area amino to carboxy terminus? Hint: the order M. Ormo, A. B. Cubitt, K. Kallio, L. A. Gross, R. Y. Tsien, S. J.
    Remington (1996) Crystal structure of the Aequorea victoria
    between strands A and K of the strands from amino to carboxy green fluorescent protein. Science 273: 1392-1395

    1
    and taping them together. terminal are marked with the numbers 1-11
    The chromophore should be (in the arrowheads of each strand).
    inside the barrel. Go to pdb101.rcsb.org to:
    2. What is the relationship between the strand

    &
    labels A-K and 1-11? Comment also on the • READ the Molecule of the Month
    loops between the strands and location of on Green Fluorescent Protein

    6 3D WATCH a video demonstration of how to build it, and to


    • DOWNLOAD additional copies of this model,
    n
    the chromophore.
    t
    y

    3. Identify one example each of parallel and access the DIGITAL ACTIVITY PAGE allowing for futher
    strand number antiparallel strands in the GFP barrel. exploaration of the 3D model (Learn > Paper Models)

    PDB-101 is the educational portal of RCSB Protein Data Bank (rcsb.org)


    Build a Paper Model of Green Fluorescent Protein (GFP)
    pdb101.rcsb.org

    K
    chromophore

    pdb101.rcsb.org

    PDB-101 is the educational portal of RCSB Protein Data Bank (rcsb.org)

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