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    F.1 Number 1 Top of The Backgraund With Mycelium Number 2: Side of The Background With Mycelium

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    Nexi anessa
    The document describes a student's research project to develop a new method of rebuilding the auricula (external part of the ear) using the Jew's ear fungus (Auricularia auricularia). The student aims to grow the fungus, decellularize it to leave just the extracellular matrix, and fill it with silicone. This would provide a cheaper and less painful alternative to current reconstruction methods. Through experiments, the student found that bleach successfully decellularized the fungus, and silicone could be added to the extracellular matrix to mimic cartilage. The student concludes that with further refinement, this fungus-based method could provide benefits over existing techniques.

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    F.1 Number 1 Top of The Backgraund With Mycelium Number 2: Side of The Background With Mycelium

    Uploaded by

    Nexi anessa
    32 views3 pages
    The document describes a student's research project to develop a new method of rebuilding the auricula (external part of the ear) using the Jew's ear fungus (Auricularia auricularia). The student aims to grow the fungus, decellularize it to leave just the extracellular matrix, and fill it with silicone. This would provide a cheaper and less painful alternative to current reconstruction methods. Through experiments, the student found that bleach successfully decellularized the fungus, and silicone could be added to the extracellular matrix to mimic cartilage. The student concludes that with further refinement, this fungus-based method could provide benefits over existing techniques.

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    The document describes a student's research project to develop a new method of rebuilding the auricula (external part of the ear) using the Jew's ear fungus (Auricularia auricularia). The student aims to grow the fungus, decellularize it to leave just the extracellular matrix, and fill it with silicone. This would provide a cheaper and less painful alternative to current reconstruction methods. Through experiments, the student found that bleach successfully decellularized the fungus, and silicone could be added to the extracellular matrix to mimic cartilage. The student concludes that with further refinement, this fungus-based method could provide benefits over existing techniques.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    32 views3 pages

    F.1 Number 1 Top of The Backgraund With Mycelium Number 2: Side of The Background With Mycelium

    Uploaded by

    Nexi anessa
    The document describes a student's research project to develop a new method of rebuilding the auricula (external part of the ear) using the Jew's ear fungus (Auricularia auricularia). The student aims to grow the fungus, decellularize it to leave just the extracellular matrix, and fill it with silicone. This would provide a cheaper and less painful alternative to current reconstruction methods. Through experiments, the student found that bleach successfully decellularized the fungus, and silicone could be added to the extracellular matrix to mimic cartilage. The student concludes that with further refinement, this fungus-based method could provide benefits over existing techniques.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    REBUILDING THE AURICULA WITH AN AURICULA AURICULARIA (JEW’S EAR)

    Meritxell Domènech Tardà

    Escola Montagut, Vilafranca del Penedès

    ABSTRACT: My idea for this research came into my mind by looking for “vegetables
    that looked like body parts” on internet. When I found a fungus called “Auricula
    Auricularia” also known as “Jew’s ear”, I realized that I could use it as a prototype to
    rebuild the auricula which is the external part of the ear.

    Nowadays there are several diseases that can make malformations on the auricula.
    There are quite few methods that can rebuild it in plastic surgery, using material provided
    by animals or also using human ribs. I think that if we used Jew’s ear as a material to
    rebuild the ear, we could allow the patient to feel less pain, and avoid rejection. Also, it
    would be inexpensive compared to current methods and finally more ethical because the
    model would be based on a fungus.

    HYPOTHESIS: My hypothesis is that if I crated a new way to build up the auricula with
    less surgical intervention and with less budget, all the patients could afford the surgery
    and also all the process would be more ethical compared to the actual one.

    OBJECTIVES: The first step is to make grown the auricula without using any type of
    additive plants because these could damage the structure of the fungus or made DNA
    mutations. That’s why a company called “Bolet ben fet” helped me by providing the basic
    background to grow the Jew’s ear.

    The second step is, to decellularize the mushroom by pulling out the fungus
    cells and leaving the extracellular matrix. Therefore, these blank spaces will be
    fitted with stem cells of the patient, thus there will not be a reason for rejection.

    After being decellularized the Auricula Auricularia, will be refill with silicone. At
    the end of the process, the scaffold would be ready to be implanted.

    METHODOLOGY: In this research I have followed the steps to rebuild the


    auricula which is the external part of the ear. The research is divided in four
    parts, the first one is to introduce the auricula and explain the process of making
    the substrate and how to make it work. To carry out this part of the project a
    f.1 NUMBER 1 TOP OF THE
    company called “Bolet Ben Fet” has helped me by providing me a background BACKGRAUND WITH MYCELIUM
    made by cereals and different types of wood. NUMBER 2: SIDE OF THE
    BACKGROUND WITH MYCELIUM
    Secondly, there is an introduction of what decellularization is and the materials
    that you need to make it at home without using specific laboratory materials.
    The process of decellularization consists in taking away all the cells except the
    extracellular matrix. This method can be used in bioengineering to make
    artificial organs. As decellularizing agents, I first tried with ethanol and acetone.
    f.2 RESULTS OF THE PROCESS
    Then, I chose bleach which is sodium hypochlorite (NaClO), vitamin C which is OF DECELLULARIZATION WITH
    BLEACH 100%
    L-ascorbic acid (C6H8O6). Finally, I used hydrochloric acid (HCl).

    Later, I improved the design of the experience by injecting silicone liquid inside
    the fungus. What I wanted in this experience was to add the part of the ear that
    usually is made with cartilage so that they are rigids. Finally, I presented the
    theoretical elements explaining how to implant the stem cell in the
    decellularized fungus. f.3 AURICULA AURICULARIA
    WITH SILICONE

    RESULTS:The fact of trying to home make a fungus at home was difficult. The return
    were not expected, the Auricula was growing too slowly so I made a few changes in the
    environment condition of the fungus, but there weren’t any results. Finally, I managed to
    get the Auricula dehydrated and rehydrated with water so I could carry out the following
    experiments.

    In the decellularization process the vitamin C and the hydrochloric acid didn’t
    decellularize all the fungus but the bleach did. Then I added silicone and from the results
    of decellularizing with bleach I realized that silicone should be added only in the
    extracellular matrix.

    CONCLUSTIONS: Working with Auricula Auricularia as a new prototype can make


    benefits at all sides, for example at belonging to bioethics because all the process is
    natural. Also, it can be the price, that is because nowadays the method of reconstruction
    of the auricula is expensive, difficult to carry on and see in the patients eyes a constant
    pain. So, what I want to explain with all of this is that using Auricula could be cheaper,
    it’d be only one operation instead of four, besides, it would be easier for the doctor to
    carry on and less pain for the patient and less probabilities to be rejected.

    To sum up, I think that if all the techniques and methods are applied in suitable conditions
    this model could be not only a prototype but it could be a new way to make better
    differences in the medical industry. Moreover, the fungus proved that can be adapted in
    different conditions, apart from the physical structure of it that proves that it is enough
    cartilaginous and strong in order to adapt easily.
    BIOGRAPHY
    Article title: Auricularia auricula; Author: unknown;Website title: ichn iec; URL:
    https://ichn.iec.cat/Bages/ribera/imatges%20grans/cAuricularia%20auricula.htm

    Article title: Auricularia auricula-judae; Author: (Bull.) Quél;Website title: Fungipedia;


    URL: https://www.fungipedia.org/hongos/auricularia-auricula-judae.html

    Article title: JEW´S EAR - AURICULARIA AURICULA-JUDAE - PURE CULTURE FOR


    ORGANIC MUSHROOM CULTIVATION ACCORDING TO REGULATION EC; Website
    title:Tyroler Glückspilze;URL: https://gluckspilze.com/Jews-Ear-Auricularia-auricula-
    judae-Pure-culture-for-organic-mushroom-cultivation-according-to-Regulation-EC-834-
    2007-and-889-2008-AT-BIO-701-Strain-No-115001

    Article title:Procedure for Decellularization of Porcine Heart by Retrograde Coronary


    Perfusion | Protocol (Translated to Spanish) ; Author:Nathaniel Remlinger ; Website title:
    Jove.com; URL:https://www.jove.com/video/50059/procedimiento-para-
    descelularizacin-de-corazn-porcino-por-perfusin?language=Spanish

    Article title: Descelularización y recelularización ;Author: Joan Tapia Llorens; Website


    title:Aplicaciones de les células madre; URL:
    http://www.aplicacionescelulasmadre.com/2014/12/descelularizacion-y-
    recelularizacion.html

    Article title: Descelularización y recelularización de órganos y tejidos; Author: Harald


    OttDoris Taylor; Website:Google Patents;
    URL:https://patents.google.com/patent/ES2384721T3/es

    Article title: Inyección intramuscular; Author: unknown; Website:Células Madre Terapia;


    URL: https://celulasmadreterapia.com/m%C3%A9todo-de-inyecci%C3%B3n/inyeccion-
    intramuscular/

    Article title: Vitamin C selectively kills KRAS and BRAF mutant colorectal cancer cells by
    targeting GAPDH; Author: Jihye Yun, Edouard Mullarky, Changyuan Lu and others;
    Website title: Science; URL: http://science.sciencemag.org/content/350/6266/1391

    Article title: Ácido clorhídrico; Website: ECURED;URL:


    https://www.ecured.cu/%C3%81cido_clorh%C3%ADdrico

    Article title:Bleach; Author: unknown; Website: Wikipedia; URL:


    https://en.wikipedia.org/wiki/Bleach

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