Determination of The Bismuth Content of Glycobiarsol 1

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TITLE
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ABSTRACT
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INTRO
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5. METHOD
The Determination of Bismuth Content of Glycobiarsol is under complexation method.
According to Kiruthiga, complexometric titration is a form of volumetric analysis in which the
formation of a colored complex is used to indicate the end point of a titration. Complexometric
titrations are particularly useful for the determination of a mixture of different metal ions in
solution using an indicator which capable of producing an unambiguous color change to detect
the end-point of the titration.
The experiment is under Residual Complexometric Titration because it has one (1) titrant
which is the Zinc Sulfate, one (1) analyte which is the Glycobiarsol and an excess of DiNaEDTA
and also the content that must be determined is Bismuth. Residual Titration is applied to the
analysis of Aluminum and Bismuth compounds, since conditions for direct titration may
introduce errors (Jenkins 1977).
Determination of Bismuth Content of Glycobiarsol
1. Instruments used
 Conical Flask – used as a container or hold the analyte

 Bunsen Burner – used to warm the analyte through waterbath.
 Beaker – used to hold the waterbath that was used to warm the flask that contain the
analyte
 Buret – used to deliver known volumes of reactant until the precise end point of the
reaction is reached.
2. Chemicals used
 Glycobiarsol – a drug that found to be very effective in intestinal infections. It is an

antiprotozoal agent that has been used in humans as well as in dogs that treats intestinal
amebiasis.
 0.05M Disodium ethylenediaminetetraacetate – a chelating agent. A chelating agent is
capable of removing a heavy metal, such as lead or mercury, from the blood. Bismuth
reacts quantitatively with EDTA, forming a stable complex.
 Diphenylthiocarbazone (Dithizone) – it is an indicator that has transition state of color at
pH 4.6. it was used to have an accurate result with a clear end point
 Alcohol ¬– it was added to ensure the solubility of the color complex because the metal
complexes of the dithizone are insoluble in water.
 0.025M zinc sulfate
 Acetic acid-ammonium acetate buffer
 Preparation – Dissolve 77.1g of Ammonium acetate in water, add 57 mL of
glacial acetic acid and dilute with water to 1000ml.
3. Procedure
 Accurately weigh 200mg of Glycobiarsol then transfer to a conical flask

 Add 10mL of 0.05M disodium ethylenediaminetetraacetate
 Warm the flask until the sample is completely dissolved and cool
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 Add 10 mL of acetic acid – ammonium acetate buffer TS

 Add 25 mL of alcohols
 Add 2 ml of dithizone TS
 Titrate the excess DiNaEDTA with 0.023M zinc sulfate until the solution is
clear rose pink in color
4. Expected Result
As the excess disodium ethylenediaminetetraacetate titrated with 0.025M of Zinc Sulfate,
the expected end point is when the solution became clear rose pink color.
5. Formula for computation
Since the principle is residual compleximetry, the formula used was:
(N x ml) − (N x ml) x meq x 100
%=
wt of the sample
The group includes the preparation and standardization of 0.05M DiNaEDTA since the group
used it as an excess:
1. Instruments used
 Buret- is used to add titrant to the titrated solution and it is designed to measure volume
of the delivered liquid.
 Beaker: used as a container and for mixing liquids.
 Conical flask- used to hold the analyte
2. Chemicals used:
 Calcium Carbonate: is used as an antacid to relieve heartburn, acid indigestion, and

upset stomach
 Disodium EDTA- is a chelating agent. Used to treat metal toxicity.
 Diluted Hydrochloric acid- solubilizes the calcium carbonate.
 Sodium hydroxide TS- a strong base, alkalinizes the solution to a pH of about 13.
 Hydroxynaphtol blue – is an azo dye. Serves as an indicator
3. Procedure:
Preparation: Add 18.6 g of Disodium Ethylenediaminetetraacetate in 1000 mL water.

Standardization
1. Weigh 200 mg of chelometric standard Calcium carbonate
2. Dissolve in 10 ml of water
3. Swirl until it forms a slurry
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4. Cover with watch glass

5. Introduce 2 mL of diluted Hydrochloric acid
6. Swirl the contents of the beaker to dissolve the Calcium carbonate
7. Dilute with 100 mL of water
8. While stirring the solution, add 30 mL of Disodium EDTA solution from 50 ml burret.
9. Add 15 mL of sodium hydroxide TS
10. Add 300 mg Hydroxynaphthol blue indicator
11. Divide the solution into 2 flask and label
12. Titrate with Disodium EDTA solution with blue end point
13. Compute for molarity.
Expected Result: the expected result for the standartization of Disodium EDTA should have the
molarity of 0.05M and the expected end point is when the analyte turns to blue color.
𝑚𝑜𝑙𝑒𝑠 𝑜𝑓 𝑠𝑜𝑙𝑢𝑡𝑒 𝑤𝑡
Formula: 𝑀 = 𝐿𝑖𝑡𝑒𝑟𝑠 𝑜𝑓 𝑠𝑜𝑙𝑢𝑡𝑖𝑜𝑛 𝑛𝑠𝑜𝑙𝑢𝑡𝑒 = 𝑚𝑤
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1

 Conical Flask – used as a container or hold the analyte

 Glycobiarsol – a drug that found to be very effective in intestinal infections. It is an

 Accurately weigh 200mg of Glycobiarsol then transfer to a conical flask

 Add 10 mL of acetic acid – ammonium acetate buffer TS

 Calcium Carbonate: is used as an antacid to relieve heartburn, acid indigestion, and

Preparation: Add 18.6 g of Disodium Ethylenediaminetetraacetate in 1000 mL water.

4. Cover with watch glass

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