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    Principios Básicos Do Desenvolvimento - Nemátoda C. Elegans

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    Gabriel Nunes da Silva
    C. elegans is a nematode that is approximately 1mm in length and consists of 959 somatic cells. It is a novel model organism established by Sydney Brenner in 1974. Robert Horvitz identified genes controlling cell death in C. elegans that also exist in mammals. John Sulston mapped the cell lineage of C. elegans, showing that specific cells undergo programmed cell death during normal development. C. elegans has many advantages as a model system, including a short generation time, ease of propagation and maintenance, and its entire cell lineage being known.

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    Principios Básicos Do Desenvolvimento - Nemátoda C. Elegans

    Uploaded by

    Gabriel Nunes da Silva
    20 views15 pages
    C. elegans is a nematode that is approximately 1mm in length and consists of 959 somatic cells. It is a novel model organism established by Sydney Brenner in 1974. Robert Horvitz identified genes controlling cell death in C. elegans that also exist in mammals. John Sulston mapped the cell lineage of C. elegans, showing that specific cells undergo programmed cell death during normal development. C. elegans has many advantages as a model system, including a short generation time, ease of propagation and maintenance, and its entire cell lineage being known.

    Original Description:

    Modelo organismo

    Original Title

    Celegans Model

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    C. elegans is a nematode that is approximately 1mm in length and consists of 959 somatic cells. It is a novel model organism established by Sydney Brenner in 1974. Robert Horvitz identified genes controlling cell death in C. elegans that also exist in mammals. John Sulston mapped the cell lineage of C. elegans, showing that specific cells undergo programmed cell death during normal development. C. elegans has many advantages as a model system, including a short generation time, ease of propagation and maintenance, and its entire cell lineage being known.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    20 views15 pages

    Principios Básicos Do Desenvolvimento - Nemátoda C. Elegans

    Uploaded by

    Gabriel Nunes da Silva
    C. elegans is a nematode that is approximately 1mm in length and consists of 959 somatic cells. It is a novel model organism established by Sydney Brenner in 1974. Robert Horvitz identified genes controlling cell death in C. elegans that also exist in mammals. John Sulston mapped the cell lineage of C. elegans, showing that specific cells undergo programmed cell death during normal development. C. elegans has many advantages as a model system, including a short generation time, ease of propagation and maintenance, and its entire cell lineage being known.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 15

    Principios básicos do desenvolvimento – Nemátoda C.

    elegans
    Position of
    C. elegans
    within the
    nematodes

    Blaxter & Koutsovoulos, 2015


    The Nobel Prize in Physiology or
    Medicine 2002
    Sydney Brenner established the nematode Caenorhabditis elegans as a
    novel model organism. This transparent worm is approximately one mm
    long and consists of 959 somatic cells (1974)

    Robert Horvitz identified genes controlling cell death in C. elegans. Corresponding


    genes exist in mammals, including man (1986)

    John Sulston mapped a cell lineage in the nematode C. elegans. He showed


    that specific cells undergo programmed cell death during the normal
    differentiation process (1977)
    `
    Introducción: Larva Dauer

    The dauer is a stage juvenile arrest that occurs during the


    development of nematodes as a response to adverse environmental
    conditions
    Main features
    •  Not fed
    •  Resistant cuticle
    •  Longevity stadium 

    Caenorhabditis elegans Ogawa, A. Science, 2009


    Juveniles
    C. elegans adulto

    Video adulto
    Hermafroditas y machos
    Hermafrodita
    El macho
    Clivaje holoblástico, asimétrico y rotacional

    PAR proteins and


    the establishment of
    polarity:
    First Cell Cycle in C. elegans
    Post-fertilización Clivaje Gastrulación

    45 min 6 horas 12 horas


    Fusión Estadío 2 Estadío 24
    pronúcleos celulas células

    Fase de proliferación

    Fase de
    morfogénesis
    C. elegans as a model system
    1.  Short generation time (3d-1week)
    2.  Stocks can be frozen
    3.  Very easy to propagate
    4.  Cheap to maintain
    5.  Temperature sensitive
    6.  Present no biohazard
    7.  First genome sequenced
    8.  All cell fates known
    9.  Easy forward and reverse genetics
    10.  RNAi
    11.  Embryos and adults translucid
    12.  Hermaphroditic and self fertile
    13.  Large brood size: 300 progeny

    Downsides: cultures smell bad and often get contaminated with fungus and
    other bacteria
    Resources
    •  Wormbase
    •  Wormbook
    •  Wormatlas
    •  Caenorhabditis Genetics Center (CGC) (U.
    Minnesota)

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