Sop Uv Vis Varian Cary50

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A.

PURPOSE
A written procedure will ensure that the analyses of samples using a
UV-VIS spectrophotometer are carried out in a consistent manner
and that consistent quality controls are also performed.

B. SCOPE
This procedure is applicable to using the Varian CARY 50 SCAN
(UV-VIS-4, UV-VIS-5) and the Varian CARY 50 BIO (UV-VIS-2, UV-VIS-
3)to measure the absorbance of Ultraviolet and Visible light in a
sample in the range of 190 – 700 nm either at single wavelengths or
as scans. This procedure only applies to the Varian CARY software
version 3.00 (182) with release date

C. TERMINOLOGY / DEFINITIONS
NOTE 1: Quartz cells (cuvettes) MUST be used in the UV range 200-400 nm because the
regular glass cells (cuvettes) absorb in this region.

NOTE 2: Use matched cells (cuvettes) for reference, samples, and standards. If matched cells
(cuvettes) are not available then use the SAME cell (cuvettes) for all measurements.

D. SAFETY

E. EQUIPMENT / MATERIALS

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